External prepn. of ox gallstone by artificial method
The present invention relates to the production method of medical medical material.
Calculus Bovis pharmaceutically is being called cattle sulphur, is the certain rare Chinese medicine of a kind of curative effect, because the source of natural Calculus Bovis is limited, so preparing Calculus Bovis with manual method is the long-term problems of exploring of people.Employed natural cattle sulphur succedaneum is artificial cattle sulphur powder at present; it is with reference to several main chemical composition preparation of natural cattle sulphur; lack some the known or unknown multiple microcomponents in the natural Calculus Bovis; the architectural feature that does not also possess natural cattle sulphur is so medical effect is not as good as natural cattle sulphur.For increasing the source of Calculus Bovis, the someone has carried out cattle body gallbladder interplantation nuclear and has cultivated the experiment of Calculus Bovis, and achieves success, but this plants the nuclear technology complexity, becomes the stone cycle long, needs could form calculus after 2 to 3 years, and need to kill cattle and get stone, therefore can not satisfy actual needs.
The objective of the invention is to explore a kind of artificial method of using, be fit to the method for the similar Calculus Bovis of medical, structure and composition and natural cattle sulphur in the external preparation of cattle, to satisfy the demand of pharmaceuticals industry to Calculus Bovis.
Realize that concrete scheme of the present invention is: in natural Fel Bovis seu Bubali, add cholic acid, deoxycholic acid, taurine, cholesterol, calcium bilirubinate, ferrous sulfate, magnesium sulfate.These proportionate relationships that add the various materials in the natural Fel Bovis seu Bubali following (weight %): cholic acid 5～12%.
Taurine 2%
Calcium bilirubinate 60～72%
Ferrous sulfate 0.5%
In natural Fel Bovis seu Bubali, add an amount of water again behind the adding above-mentioned substance and stir into suspension.(ratio of the total amount of the various materials in the natural Fel Bovis seu Bubali of above-mentioned adding and used natural Fel Bovis seu Bubali and water consumption is: every 100g adds material with 20 to 60ml natural Fel Bovis seu Bubali and 50 to 200ml water.) regulate the pH value of suspension again with hydrochloric acid, make its pH value below 6.8, rotate or rock the container of splendid attire suspension then, make the suspension in it in container, make unidirectional rotary motion, rotate and calculus can occur after 15 to 20 minutes.Standby after the taking-up calculus drying.
Fig. 1 is the flow chart of this inventive method.
Embodiments of the invention: get natural Fel Bovis seu Bubali 60ml; add cholic acid 12g, deoxycholic acid 10g, taurine 2g, cholesterol 2g, calcium bilirubinate 68g, ferrous sulfate 0.5g, magnesium sulfate 1g, add water 100ml stirring becoming suspension; be adjusted to below 6.8 with the pH value of hydrochloric acid suspension; the container of splendid attire suspension is rotated on the plane then; make the suspension in it make unidirectional rotary motion in container, rotating promptly has calculus to form after 15 to 20 minutes.Calculus is taken out, and vacuum drying is standby.
In this way the Calculus Bovis of Zhi Zuoing is not only identical with natural cattle sulphur in appearance, and through electron microscopic observation, its microstructure is also basic identical with natural Calculus Bovis.Through the medicine inspection analysis; infrared spectrum and atomic absorption spectroscopy; its Main Ingredients and Appearance and content and natural cattle sulfo group are originally identical; and contain 18 kinds of trace element such as Ca, Mg, Fe, Na, P, Cu, Zn and the contained trace element of natural cattle sulphur basic identical; this shows in the process of in this way producing calculus; some microcomponents in the natural Fel Bovis seu Bubali have also participated in the formation of calculus, have been brought in the calculus, and this artificial just cattle sulphur powder is lacked.Aspect curative effect, prove that through zoopery the antiinflammatory of this artificial Calculus Bovis, convulsion and antipyretic effect are similar to natural cattle sulphur, stronger than artificial cattle sulphur powder.