CN102247137B - Microelectrode array-based multichannel neural information detection system - Google Patents

Microelectrode array-based multichannel neural information detection system Download PDF

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CN102247137B
CN102247137B CN 201010183442 CN201010183442A CN102247137B CN 102247137 B CN102247137 B CN 102247137B CN 201010183442 CN201010183442 CN 201010183442 CN 201010183442 A CN201010183442 A CN 201010183442A CN 102247137 B CN102247137 B CN 102247137B
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CN102247137A (en
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林楠森
蔡新霞
宋轶琳
刘春秀
罗金平
刘军涛
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中国科学院电子学研究所
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Abstract

本发明公开了一种基于微电极阵列的多通道神经信息检测系统,涉及神经生物学检测技术,包括模拟开关阵列,电生理信号检测电路,电化学信号检测电路,电刺激信号发生电路,多路AD转换电路,温度检测电路,高速USB传输电路,无线蓝牙通讯模块和计算机上运行的数据处理软件等。 The present invention discloses a method based on multi-channel information detection nervous system microelectrode array, related to neuro biology techniques, including analog switch array, electrophysiological signal detection circuit, the electrochemical signal detection circuit, electrical stimulation signal generating circuit, the multiplexer AD conversion data processing software running on a circuit, a temperature detection circuit, high-speed USB transfer circuit, and a computer wireless Bluetooth communication module. 本发明多通道神经信息检测系统可用于在体动物或离体组织的电生理和电化学神经信息获取,其结合不同种类的微电极阵列可在二维或三维尺度上对多个神经元的电生理信号和电化学信号进行检测,获得更为全面和准确的实验数据,实现神经元双向双模的应用实施。 Multi-channel information detection nervous system of the present invention may be used to obtain or animal body from electrochemical and electrophysiological nerve tissue in the body of information, which binds to different types of micro-electrode array may be electrically plurality of neurons in two or three dimensions physiological and electrochemical signals to detect signals, to obtain a more comprehensive and accurate test data to achieve two-way neurons dual-mode application implementation.

Description

基于微电极阵列的多通道神经信息检测系统 Multi-channel information detection nervous system microelectrode array

技术领域 FIELD

[0001] 本发明涉及神经生物学检测技术领域,是一种用以同时检测多个神经元电生理活动以及神经递质变化的检测系统。 [0001] The present invention relates to the field of neurobiology detection, for simultaneously detecting a plurality of electrical activity of neurons and neurotransmitters in the detection system.

背景技术 Background technique

[0002] 神经细胞的信息传递由脉冲放电和神经递质(化学物质)共同完成,同时检测电生理信号和神经递质含量变化,籍以研究某些疾病的发病机理、神经信号传递、药物反应等具有重要科学意义和临床价值。 Information transfer [0002] nerve cell by a pulse discharge and the completion of the neurotransmitter (chemical substances) together, simultaneously and the detection signal changes in neurotransmitter content electrophysiology, membership in order to study the pathogenesis of certain diseases, nerve signal transduction, drug reactions It has important scientific significance and clinical value. 同时生物体对于外界事件的响应以及信息处理的每一个过程,都需要涉及许多神经细胞的共同作用,要揭示这其中的复杂机制,必须获得足够数目的神经细胞的电活动信息。 Meanwhile organisms respond to external events and every process of information processing, we need joint action involving many nerve cells, to reveal the complex mechanisms of which must be obtained information sufficient number of electrical activity of nerve cells. 传统的电极或单个微电极技术已不能满足临床和科研检测需求。 Conventional electrode or microelectrode single technology can not meet the needs of clinical and scientific testing. 因此对多个脑神经元的电活动和神经递质信息进行同步记录可以为神经科学研究提供一种便利、可靠的信息获取工具,对于神经科学基础研究、神经疾病治疗(如癫痫,帕金森氏症)、脑机接口、高通量药物筛选和安全药理学等领域具有非常重要的意义。 Therefore, a plurality of electrical activity of neurons and neurotransmitters information to synchronize records can provide a convenient, reliable information for neuroscience research to obtain tools for basic research in neuroscience, neurological treatment of diseases (such as epilepsy, Parkinson's disease), brain-computer interface, high-throughput drug screening and safety pharmacology and other fields has a very important significance.

发明内容 SUMMARY

[0003] 本发明的目的是公开一种基于微电极阵列的多通道神经信息检测系统,通过计算机的软件控制微电极阵列的功能排布,以满足不同实验的要求,并同时检测神经电生理和电化学信号,以解决神经细胞的连续、多通道检测和微弱电生理和电化学信号的检测问题。 [0003] The object of the present invention is to disclose a multi-channel nerve-based information detecting system in microelectrode array, controlled by computer software microelectrode array features arranged to meet the requirements of different experiments, and simultaneous detection and neurophysiological electrochemical signals, to resolve a continuous, multi-channel detection problem detection and weak electrochemical signal and electrophysiological nerve cells.

[0004] 为达到上述目的,本发明的技术解决方案是: [0004] To achieve the above object, the technical solution of the invention is:

[0005] 一种基于微电极阵列的多通道神经信息检测系统,其结合模拟开关阵列实现电极的双向功能切换,并通过电生理信号检测模块和电化学信号检测模块实现神经元的双模多通道同步检测; [0005] Based on the multi-channel information detection nervous system microelectrode array, which binds analog switch array electrode to achieve bidirectional switching function, and to realize neuron electrophysiological signal detection module and the detection module of the dual mode electrochemical signals Multichannel synchronization detection;

[0006] 多通道神经信息检测系统,包括:中央处理器单元,协处理器单元,模拟开关阵列,温度监测电路,刺激信号产生模块,电生理信号检测模块,电化学信号检测模块,多通道并行采集模块,高速缓存电路,高速USB通信接口,无线蓝牙通讯接口和计算机软件;其中,中央处理器单元连接多通道并行采集模块、高速缓存电路、协处理器单元,并通过高速USB芯片与计算机进行通信;协处理器单元连接无线蓝牙通讯接口、电生理信号检测模块、模拟开关阵列、刺激信号产生模块、电化学检测模块、温度监测电路;模拟开关阵列连接刺激信号产生模块、电化学检测模块、微电极阵列、并通过电生理信号检测模块与并行采集模块电连接; [0006] Multi-channel information detection nervous system, comprising: a central processor unit, a coprocessor unit, the analog switch array, a temperature monitoring circuit, the stimulation signal generating module, electrophysiological signal detection module, the electrochemical signal detection module, the multi-channel parallel acquisition module, cache circuit, a high-speed USB communication interface, a Bluetooth wireless communication interface and computer software; wherein a central processor unit coupled to a multichannel parallel acquisition module, cache circuit, a coprocessor unit, and high-speed USB chip computer communication; coprocessor unit connected to a Bluetooth wireless communication interface, electrophysiological signal detection module, the analog switch array, the stimulation signal generation module, electrochemical detection module, the temperature monitoring circuit; an analog switch array connected to a stimulation signal generating module, electrochemical detection module, microelectrode array, and electrically connected by an electrical physiological signal detection module and the parallel acquisition module;

[0007] 微电极阵列与温度监测电路电连接。 [0007] The electrode array is electrically connected to the temperature monitoring circuit.

[0008] 所述的多通道神经信息检测系统,其所述电极的双向功能,为检测与刺激功能;神经元的双模检测,为电生理和电化学信息检测;电生理信号检测模块包括伪迹消除电路和前置放大器电路,协处理器单元和模拟开关阵列分别与伪迹消除电路连接,伪迹消除电路经前置放大器电路与中央处理器单元连接,通过控制模拟开关阵列的导通状态,以对每个电极的使用功能进行选择。 [0008] The multi-channel information detection nervous system function of said bidirectional electrode, to detect a stimulus function; dual-mode detection neurons for electrophysiological and electrochemical detection information; electrophysiological signal detection module includes a pseudo- trace preamplifier circuit and the cancellation circuit, the coprocessor unit and an analog switch array respectively elimination circuit connected to the artifact, artifact removal circuit connected via a preamplifier circuit and a central processing unit, by controlling the conductive state of the analog switch array to be selected using the function of each electrode. [0009] 所述的多通道神经信息检测系统,其所述模拟开关阵列,由计算机软件控制,对微电极阵列所连接的模拟开关阵列进行设置,从而使每个电极选择不同的用途,分别连接到电生理信号检测模块、电化学信号检测模块、刺激信号产生模块,参考或地,由此根据具体的实验要求,方便的更改微电极阵列的功能排布。 [0009] The multi-channel information detection nervous system, the analog switch array which is controlled by computer software, the analog switch array is connected to the microelectrode array is arranged such that each electrode selected different purposes, are connected the electrophysiological signal detection module, the electrochemical signal detection module, the stimulation signal generation module, or to a reference, whereby according to the specific experimental requirements, easy to change the function microelectrode array arrangement.

[0010] 所述的多通道神经信息检测系统,其所述电化学检测模块与电生理检测模块配合,通过模拟开关阵列的控制,以选择微电极阵列上的每个电极做电化学检测或做电生理检测,从而实现检测位点设置的电化学信号和电生理信号多通道同步检测。 [0010] The multi-channel information detection nervous system and the electrochemical detection module with electrophysiological detection module, by controlling the analog switch array to select each electrode in the microelectrode array do or do electrochemical detection electrophysiological testing, in order to achieve the electrochemical signal and the multichannel detector electrophysiological signal synchronization detection site is provided.

[0011] 所述的多通道神经信息检测系统,其所述无线蓝牙通讯接口和协处理器单元相连,计算机直接通过协处理器单元来设置各模块的参数,避免占用USB的带宽,从而增强了系统的响应速度,使得系统在处理庞大数据量的同时,及时响应计算机的指令。 [0011] The multi-channel detection system of nervous information that the Bluetooth wireless communication interface and a coprocessor unit is connected directly to the computer to set the parameters of each module via coprocessor unit, avoid occupying USB bandwidth, thereby enhancing the response speed, so that the system while processing large amounts of data in a timely manner in response to commands from the computer.

[0012] 所述的多通道神经信息检测系统,其所述协处理器单元,采用高速32位ARM芯片,通过电化学模块检测神经递质的含量,并协调系统内各模块之间的工作,减轻中央处理器的工作量,提高系统的实时响应速度。 [0012] The multi-channel information detection nervous system of the coprocessor unit, a high-speed 32-bit ARM chip, neurotransmitter content by electrochemical detection module, and coordination between the various modules in the system, reduce the workload of the central processor, to improve the real-time response speed of the system.

[0013] 所述的多通道神经信息检测系统,其所述刺激信号产生模块,采用双电源供电的高精度数模转换芯片,实现频率、幅度和波形自定义的双极性电压或电流波形,使刺激后电极上的净电荷为零,降低对神经细胞的损伤,同时避免剩余电荷对电极的电解,延长电极的使用寿命。 [0013] The multi-channel detection system of nervous information that said stimulation signal generating module, a dual power supply with high precision digital-analog converter chip, the frequency, amplitude and waveform from the bipolar voltage or current waveforms defined, after allowing the stimulation zero net charge on the electrode, to reduce damage to nerve cells, while avoiding residual charges electrolysis electrodes, extend the life of the electrode.

[0014] 所述的多通道神经信息检测系统,其所述双极性电压或电流波形,是采用二选一的多路开关来实现,多路开关由计算机软件进行控制。 [0014] The multi-channel information detection nervous system, which the bipolar voltage or current waveform, is the use of two selected from a multiplexer implemented, the multiplexer is controlled by a computer software.

[0015] 所述的多通道神经信息检测系统,其所述温度检测电路,采用灵敏度为0.1°C的微型温度传感器,以实现对离体组织培养环境的准确实时监测,避免由于温度过低或过高带来的组织活性降低。 [0015] The multi-channel information detection nervous system which the temperature detecting circuit using a sensitivity of 0.1 ° C the micro temperature sensor, in order to achieve Tissue culture accurate real-time monitoring environmental temperature is too low or avoid organization of too much activity.

[0016] 本发明的神经信息检测系统,可用于在体动物或离体组织的电生理和电化学神经信息获取。 [0016] The present invention nervous information detecting system can be used to obtain an animal or in vivo electrochemical and electrophysiological information vitro neural tissue. 其结合不同种类的微电极阵列,如适于在体神经信息检测的Utah电极、Michigan电极和适于离体神经信息检测的平面MEA电极,可在二维或三维尺度上对多个神经元的电生理信号和电化学信号进行检测,获得更为全面和准确的实验数据,实现神经元双向(检测与施加电刺激)双模(神经元电生理信号与电化学信号)的应用实施。 Which binds to different types of microelectrode array, as adapted to the detected information Utah nerve electrode, Michigan planar electrode and MEA electrode adapted neural information from the sample, a plurality of neurons may be in two or three dimensions on the scale of electrophysiological signals and electrochemical signals are detected, to obtain a more comprehensive and accurate experimental data to achieve dual-mode bidirectional neuronal (neuron electrophysiological signals electrochemical signal) applied embodiment (detection of an electrical stimulus).

附图说明[0017] 图[0018] 图[0019] 图[0020] 图阅图; 图[0021] [0022] 图[0023] 图[0024] 图具体实施方式 BRIEF DESCRIPTION [0017] FIG [0018] FIG [0019] FIG [0020] FIG. See Figure; Fig [0021] [0022] FIG [0023] FIG. [0024] DETAILED DESCRIPTION FIG.

[0025] 本发明公开一种基于微电极阵列(如图4)的多通道神经信息检测系统,其结合模拟开关阵列可以实现电极的双向(检测与刺激)功能切换,并通过电生理信号检测模块和电化学信号检测模块实现神经元的双模(电生理和电化学信息)多通道同步检测。 [0025] The present invention discloses a multi-channel information detection system based on neural microelectrode array (FIG. 4), the analog switch array may be implemented in conjunction with bi-directional electrode (detection and stimulation) switching function, electrophysiological and signal detection module the electrochemical signal detection module and achieve bimodal neurons (electrochemical and electrophysiological information) multi-channel synchronous detection. 如图5所示,系统包括中央处理单元,协处理器单元,模拟开关阵列,温度监测电路,刺激信号产生电路,电生理检测模块,电化学检测模块,多通道并行采集模块,高速缓存电路,高速USB通信接口,无线蓝牙通讯接口和计算机软件。 As shown, the system includes a central processing unit 5, a coprocessor unit, the analog switch array, a temperature monitoring circuit, a stimulation signal generating circuit, electrophysiological testing module, electrochemical detection module, the multi-channel parallel acquisition module, the cache circuit, High-speed USB communication interface, wireless Bluetooth communication interface and computer software. 其中,中央处理单元连接多通道采集模块,高速缓存电路,并通过高速USB芯片与计算机进行通信;协处理器单元连接电化学检测模块,温度监测电路,刺激信号产生电路,模拟开关阵列,伪迹消除电路,无线蓝牙通讯接口;电生理信号检测模块由伪迹消除电路和前置放大器电路组成。 Wherein the central processing unit is connected to the multi-channel acquisition module, the cache circuit, and communicates with the computer via a high speed USB chip; coprocessor module unit is connected electrochemical detection, temperature monitoring circuit, a stimulation signal generating circuit, an analog switch array, artefacts elimination circuit, a Bluetooth wireless communication interface; electrophysiological signal detection circuit and a preamplifier circuit module by the artifact composition.

[0026] 本发明的模拟开关阵列是通过如图2所示的原理框图实现的,微电极阵列的引线连接到模拟开关阵列,通过计算机软件可以控制各开关的状态,从而控制各个电极的功能模式,这样同一个电极分别可以复用为电生理信号检测电极、电化学信号检测电极、电刺激电极、参考电极、地电极。 [0026] The analog switch array according to the present invention is achieved by the principle shown in block diagram in FIG. 2, the lead microelectrode array connected to the analog switch array, each of the switching may be controlled by the state of the computer software to control the functional mode of each electrode , so that the same may be multiplexed to each electrode electrophysiological signal detection electrode, the electrochemical signal detection electrode, the electrical stimulation electrodes, a reference electrode, a ground electrode. 其中软件采用Visual C++6.0和Keil C编写,控制界面及流程如图7、8所示。 Wherein the software using Visual C ++ 6.0 Keil C and prepared, and process control interface 7 and 8 as shown in FIG. 实验时可根据具体的要求来灵活的调整微电极阵列的排布,可选择任意一个电极对感兴趣的组织区域进行检测或刺激,从而极大的提高了检测的空间分辨率,可以获取更加有价值的信号。 Experiment according to the specific requirements flexibly adjust the arrangement of the microelectrode array, select any one electrode of the tissue region of interest is detected or stimulation, which greatly improves the spatial resolution of detection, can be acquired more signal value.

[0027] 本发明的前置放大电路是通过图3所示电路实现的,该电路采用差分输入形式,选择具有高共模抑制比的仪表放大器AD620作为第一级放大,为了避免电容的不匹配带来的共模抑制比下降,采用的是直流耦合方式来放大微电极信号,但由于在体或离体的实验中都会有几十到几百毫伏的直流极化电压存在,为避免放大器饱和,放大倍数设置为10倍左右,在仪表放大器的后面连接高通滤波器以滤除直流信号,可根据具体要求设置截止频率的大小,一般设为0.1〜IOHz,高通滤波器后面接截止频率为3KHz左右的低通滤波器,以滤除高频噪声,保证记录到神经信号具有高信噪比,如图6所示。 [0027] The pre-amplifier circuit of the present invention is realized by a circuit shown in FIG. 3, the form of the differential input circuit, that has a high common-mode rejection ratio AD620 instrumentation amplifier as a first amplifying stage, in order to avoid capacitance mismatch CMRR brought down DC coupling is used to amplify the signal microelectrodes, but because of the polarization voltage or a DC test from the body will have tens to hundreds of millivolts, to avoid the amplifier saturated, the magnification is set to about 10 times, after the high-pass filter connected to the instrumentation amplifier to filter the DC signal, according to the specific requirements of the cutoff frequency of the size, generally set 0.1~IOHz, behind the high-pass filter cut-off frequency of contact about 3KHz low-pass filter to filter out high frequency noise, to ensure that the recording signal has a high signal to noise ratio nerve, as shown in FIG. 根据不同类型的神经细胞放电大小不同,还可以在后面接多级放大以调整总的放大倍数,一般为1000〜5000倍。 Depending on the size of the different types of neural discharge cells, may also be connected to the rear multi-stage amplifier to adjust the overall magnification, is generally 1000~5000 times.

[0028] 对于单极性的刺激脉冲,会对电极进行充电,需要一段时间的放电,由此会在记录时加入放电伪迹,并且也加速了电极在组织液或培养液中的电解,缩短电极的使用寿命。 [0028] For unipolar stimulation pulses, electrodes for charging will take some time to discharge, whereby the discharge artifacts will be added during the recording, and also accelerated fluid or tissue culture electrode in an electrolytic solution, an electrode shortening life. 针对不同的实验对象和不同类型的电极,刺激信号的大小和种类也不同,本发明设计了一种能够产生双极性刺激信号的电路,所产生的刺激信号脉冲包括正脉冲和负脉冲,因而可以保证施加在电极上的净电荷为0,能够避免由传统刺激信号所带来的诸多不良影响,并且所产生的刺激信号是可由用户自定义参数的。 For different subjects and different types of electrode stimulation signals are different size and type, the present invention designs a circuit bipolar stimulation signal can be generated, the generated stimulation signal comprises positive pulses and negative pulses, thus It ensures that the net charge applied to the electrodes is 0, can avoid the many adverse effects caused by the conventional signal stimulus, and the resulting signal is a user definable parameter. 如图1所示,信号波形由工作于+/-5V的10位DAC芯片MAX504产生,应而可以产生双极性(包括正脉冲和负脉冲)的波形,刺激信号的参数:脉冲宽度、脉冲大小、脉冲形状、脉冲个数、间隔时间等,可通过芯片的2号管脚DIN进行设置。 1, the signal waveforms in the 10-bit DAC chip MAX504 +/- 5V is generated by the working, but may be a bipolar waveform generated (including positive and negative pulse), the stimulation signal parameters: pulse width, pulse size, pulse shape, pulse number, interval, etc., can be provided through a No. 2 pin DIN chip. MAX504的输出信号经过运算放大器AD8674跟随电压后分出两路,其中一路接V/I转换电路,由R1-R5和两个运算放大器组成,其中输出电流由电阻R4决定;另一路接电压放大电路,由R6、R7和一个运算放大器组成,以调整输出电压刺激的量程(大小由R6、R7的比值确定),这两路信号可接到一个2选I的开关,最终决定输出是电压刺激信号还是电流刺激信号。 MAX504 output signal of the operational amplifier through a voltage follower AD8674 separation after two, then the way in which the V / I converting circuit, and two of R1-R5 operational amplifier, wherein the output current is determined by the resistor R4; another way then the voltage amplifier circuit by R6, R7 and an operational amplifier to adjust the output voltage range of stimulation (the size determined by the ratio of R6 R7), two signals may be selected to the switch I is a 2, the final decision is the output voltage of the stimulus signal or the current stimulation signal.

[0029] 以上所述,仅为本发明的优选实施方式。 [0029] The above are only preferred embodiments of the present invention. 应当指出,对于本领域的技术人员依据本发明的创造原理,可以做出许多变形和改进,但这些均落入本发明权利要求的保护范围。 It should be noted that those skilled in the art in accordance with principles of the present invention to create, many variations and modifications may be made, but these fall within the scope of the claims of the invention.

Claims (6)

1.一种基于微电极阵列的多通道神经信息检测系统,其特征在于,包括:中央处理器单元,协处理器单元,模拟开关阵列,温度监测电路,刺激信号产生模块,电生理信号检测模块,电化学信号检测模块,多通道并行采集模块,高速缓存电路,高速USB通信接口,无线蓝牙通讯接口和计算机软件; 所述中央处理器单元连接所述多通道并行采集模块、所述高速缓存电路和所述协处理器单元,并通过所述高速USB通信接口与计算机进行通信;所述协处理器单元连接所述无线蓝牙通讯接口、所述电生理信号检测模块、所述模拟开关阵列、所述刺激信号产生模块、所述电化学信号检测模块和所述温度监测电路;所述模拟开关阵列连接所述刺激信号产生模块、所述电化学信号检测模块和所述微电极阵列,并通过所述电生理信号检测模块与所述多通道并行采集模块电 1. Based on the multi-channel information detection nervous system microelectrode array, characterized by comprising: a central processor unit, a coprocessor unit, the analog switch array, a temperature monitoring circuit, the stimulation signal generating module, electrophysiological signal detection module electrochemical signal detection module, the multi-channel parallel acquisition module, cache circuit, a high-speed USB communication interface, a Bluetooth wireless communication interface and computer software; the central processor unit connected to the multichannel parallel acquisition module, the cache circuit unit and said coprocessor, and said high-speed USB communication interface through communication with the computer; the coprocessor unit is connected to the Bluetooth wireless communication interface, the electrophysiological signal detection module, the analog switch array, the said stimulation signal generating module, the electrochemical signal detection module and the temperature monitoring circuit; said analog switch array connected to the stimulus signal generating module, the electrochemical signal detection module and the microelectrode array, and by the said electrophysiological signal detection module and the multi-channel parallel electrical collection module 连接;所述微电极阵列与所述温度监测电路电连接所述多通道神经信息检测系统结合所述模拟开关阵列实现所述微电极阵列中每个电极的双向功能切换,即通过计算机软件控制所述模拟开关阵列的开关状态,使得所述微电极阵列中的每个电极连接到所述电生理信号检测模块、所述电化学信号检测模块以及所述刺激信号产生模块,并通过所述电生理信号检测模块和所述电化学信号检测模块实现神经元的双模多通道同步检测,并通过所述刺激信号产生模块实现对神经元的电学激励; 其中,所述电极的双向功能,为检测与刺激功能;所述神经元的双模多通道同步检测,为电生理和电化学信息检测;所述电生理信号检测模块包括伪迹消除电路和前置放大器电路,所述协处理器单元和所述模拟开关阵列分别与所述伪迹消除电路连接,所述伪迹消除电路经前置 ; Said microelectrode array and the temperature-monitoring circuit electrically connected to the multi-channel information detection nervous system combines the analog switch array implementing the microelectrode array of bidirectional capability of each electrode switching, i.e. by software controlling the computer said switching state of the analog switch array, such that each electrode of the micro-electrode array connected to the electrical physiological signal detection module, the electrochemical signal detection module and said stimulation signal generating means, and through said electrophysiological signal detection module and a module for the electrochemical signal detection neuron dual mode multi-channel synchronous detection, and by the stimulation signal generation module implemented on the electrical excitation of neurons; wherein said bi-functional electrode, to detect a stimulation function; the neuron dual mode multi-channel synchronous detection, electrochemical and electrophysiological information is detected; electrophysiological signal detection module comprises artifact circuit and preamplifier circuit, and the unit of the coprocessor said analog switch array respectively cancellation circuit connected to the artifact, artifact from the pre-circuit is 放大器电路与中央处理器单元连接,通过控制模拟开关阵列的导通状态,以对每个电极的使用功能进行选择; 所述模拟开关阵列,由计算机软件控制,使得所述微电极阵列中的每个电极在所述模拟开关阵列的导通或切断下连接到以下三个所述模块:电生理信号检测模块、电化学信号检测模块、刺激信号产生模块,由此根据具体的实验要求,方便的更改微电极阵列的功能排布; 所述电化学信号检测模块与所述电生理信号检测模块配合,通过模拟开关阵列的控制,以选择微电极阵列上的每个电极做电化学检测或做电生理检测,从而实现检测位点设置的电化学信号和电生理信号多通道同步检测。 The amplifier circuit is connected to the central processor unit, to be selected by using the function of each electrode to control the conductive state of the analog switch array; the analog switch array, controlled by computer software, the micro-electrode array such that each of electrodes connected in said analog switch is turned on or off of the array to the three of the following modules: an electrophysiological signal detection module, the electrochemical signal detection module, the stimulation signal generation module, thus according to the specific experimental requirements, easy change microelectrode array features arranged; the electrochemical signal detection module and the detection module with the electrical physiological signal, by controlling the analog switch array to select each electrode in the microelectrode array do or do electrical electrochemical detection physiological detection, thereby realizing an electrochemical signal and the multichannel detector electrophysiological signal synchronization detection site is provided.
2.如权利要求1所述的多通道神经信息检测系统,其特征在于,所述无线蓝牙通讯接口和所述协处理器单元相连,计算机直接通过所述协处理器单元来设置所述刺激信号产生模块、电化学信号检测模块和温度监测电路的工作状态,避免占用USB的带宽,从而增强了系统的响应速度,使得系统在处理庞大数据量的同时,及时响应计算机的指令。 2. The multi-channel information detecting system according nervous claimed in claim 1, wherein the Bluetooth wireless communication interface coupled to the coprocessor and the unit computers of the stimulation signal is provided directly by means of the coprocessor generating module, the operating state electrochemical signal detection module and temperature monitoring circuits, to avoid the USB bandwidth occupied, thereby enhancing the response speed of the system, so that the system while processing a large amount of data, in response to commands from the computer in time.
3.如权利要求1或2所述的多通道神经信息检测系统,其特征在于,所述协处理器单元,采用高速32位ARM芯片,通过所述电化学信号检测模块检测神经递质的含量,并协调系统内各模块之间的工作,减轻所述中央处理器单元的工作量,提高系统的实时响应速度。 3. The multi-channel information detection nervous system of claim 1 or claim 2, wherein said coprocessor unit, a high-speed 32-bit ARM chip, neurotransmitter content detected by the electrochemical signal detection module and coordination between the various modules in the system, reducing the workload of the central processing unit, to improve the response speed of the system in real time.
4.如权利要求1所述的多通道神经信息检测系统,其特征在于,所述刺激信号产生模块,采用双电源供电的高精度数模转换芯片,实现频率、幅度和波形自定义的双极性电压或电流波形,使刺激后所述电极上的净电荷为零,降低对神经细胞的损伤,同时避免剩余电荷对所述电极的电解,延长所述电极的使用寿命。 4. The multi-channel information detecting system according nervous claimed in claim 1, characterized in that said stimulation signal generating module, a dual power supply with high precision digital-analog converter chip, the frequency, amplitude and waveform custom bipolar of the voltage or current waveform, so that the net charge on the stimulating electrode is zero, reducing the damage to nerve cells, while avoiding the residual charge of the electrolysis electrodes, extend the life of the electrode.
5.如权利要求4所述的多通道神经信息检测系统,其特征在于,所述双极性电压或电流波形,是采用二选一的多路开关来实现,所述多路开关由计算机软件进行控制。 5. The multi-channel information detection nervous system of claim 4, wherein said bipolar voltage or current waveform, is the use of two selected from a multiplexer to implement the multi-way switch by a computer software control.
6.如权利要求1所述的多通道神经信息检测系统,其特征在于,所述温度监测电路,采用灵敏度为0.1°C的微型温度传感器,以实现对离体组织培养环境的准确实时监测,避免由于温度过低或过高带来的所述组织活性降低。 The multi-channel information detecting system according nervous claimed in claim 1, wherein said temperature monitoring circuit, sensitivity 0.1 ° C using a miniature temperature sensor for accurate real-time monitoring of the ex vivo tissue culture environment, the temperature is too low to avoid or reduce the activity of too much tissue.
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