CN102212472A - Automatic rotary microbial spore incubator - Google Patents
Automatic rotary microbial spore incubator Download PDFInfo
- Publication number
- CN102212472A CN102212472A CN2011100679288A CN201110067928A CN102212472A CN 102212472 A CN102212472 A CN 102212472A CN 2011100679288 A CN2011100679288 A CN 2011100679288A CN 201110067928 A CN201110067928 A CN 201110067928A CN 102212472 A CN102212472 A CN 102212472A
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- incubator
- outer case
- automatic rotary
- box body
- temperature
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000003860 storage Methods 0.000 claims abstract description 13
- 238000010438 heat treatment Methods 0.000 claims abstract description 11
- 239000000523 sample Substances 0.000 claims abstract description 10
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 abstract description 6
- 230000004151 fermentation Effects 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 238000011534 incubation Methods 0.000 abstract 4
- 235000015099 wheat brans Nutrition 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 238000011169 microbiological contamination Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses an automatic rotary microbial spore incubator. The incubator contains a rack, a sterile air adding tube, a steam adding tube, a vent pipe, an incubation box, an electric motor controller, an electric motor, a reduction gearbox and a temperature controller. The automatic rotary microbial spore incubator is characterized in that the incubation box is composed of an outer box body and an inner box body; a water storage cavity is between the outer box body and the inner box body; a heating rod is fixed in the water storage cavity; the inner wall of the incubation cavity is provided with a temperature-sensing probe; the heating rod and the temperature-sensing probe are connected with the temperature controller through wires; rotation shafts on the outer walls of both sides of the outer box body are fixed in two positioning bases of the rack through bearings respectively; the driving shaft of the reduction gearbox is fixedly connected with one rotation shaft on the outer box body; and the end part of the vent pipe passes through the other rotation shaft of the outer box body to enter the incubation cavity. By adopting the automatic rotary microbial spore incubator, spores can be produced automatically; and the produced spores have uniform and stable quality and can be widely used in microbial fermentation production enterprises.
Description
Technical field
The present invention relates to the spore culture apparatus in a kind of microbial fermentation production process, specifically a kind of automatic rotary microbial spore incubator is applicable to the enlarged culturing of bacterial classification in the microbial fermentation.
Background technology
In microbial fermentation, the enlarged culturing mode of bacterial classification generally adopts the mode of slant culture → triangular flask wheat bran cultivation → seed tank culture → ferment tank, the weak point of this method is: the incompatibility large-scale industrial production, use a large amount of triangular flasks, triangular flask is easily broken, and loss is big, simultaneously because wheat bran spore demand is big, manually turn over bottle, need manpower many, labour intensity is big, and all swivel links are many, increased the microbiological contamination chance, every batch of spore quality can not keep stable, also uneven with a collection of miospore quality, when carrying out the shaking table detection, representativeness is also bad, and therefore this training method is seriously restricting the development of microbial fermentation industry.
Summary of the invention
The object of the present invention is to provide a kind of simple in structurely, easy to operate, full-automatic operation can reduce the microbiological contamination chance, and is time saving and energy saving, can reduce labour intensity, guarantees the automatic rotary microbial spore incubator of spore quality stable homogeneous.
In order to reach above purpose, the technical solution adopted in the present invention is: this automatic rotary microbial spore incubator, comprise frame, sterile air adds pipe, steam adds pipe, ventpipe, incubator, electric machine controller, motor, reduction box and temperature regulator, the top of incubator is provided with charging opening, the bottom of incubator is provided with drain hole, sterile air adds pipe and steam adding pipe is connected with ventpipe respectively, motor and reduction box are fixed on the frame, motor is connected with reduction box, motor is connected with electric machine controller by lead, it is characterized in that: described incubator is made of outer case and internal box, outer case is sheathed and be fixed on the outside of internal box, it between outer case and the internal box water storage cavity, fill water in the water storage cavity, be provided with heating rod at the water storage cavity internal fixing, the inner chamber of internal box is a culture chamber, charging opening and drain hole are connected with culture chamber respectively, on the inwall of culture chamber, be provided with temperature-sensing probe, heating rod is connected with temperature regulator by lead respectively with temperature-sensing probe, on frame, be arranged with two positioning seats, the rotating shaft of the both sides outer wall of outer case respectively by bearing fixing in two positioning seats, the drive shaft of reduction box is fixedlyed connected with a rotating shaft on the outer case, and the end of ventpipe enters in the culture chamber after passing another rotating shaft of outer case.
The present invention also implements by following measure: described outer case and internal box are the circular spheroid of stainless steel.
All be provided with cock on described charging opening and the drain hole.
Described temperature regulator fixedly is located on the outer wall of outer case; Described electric machine controller fixedly is located on the frame.
Beneficial effect of the present invention is: compare with present triangular flask wheat bran spore training method, realized the automated production spore, alleviated labour intensity, reduced manpower, reduced the microbiological contamination chance, the spore quality stable homogeneous of making adapts to large-scale industrial production, can be widely used in microbial fermentation manufacturing enterprise.
Description of drawings
Fig. 1 is a structure partial cross-sectional schematic of the present invention.
Among the figure: 1, frame; 2, sterile air adds pipe; 3, steam adds pipe; 4, ventpipe; 5, electric machine controller; 6, motor; 7, reduction box; 8, drive shaft; 9, positioning seat; 10, outer case; 11, internal box; 12, charging opening; 13, drain hole; 14, heating rod; 15, water storage cavity; 16, temperature-sensing probe; 17, culture chamber; 18, temperature regulator.
Embodiment
Make the present invention with reference to accompanying drawing 1.This automatic rotary microbial spore incubator, comprise frame 1, sterile air adds pipe 2, steam adds pipe 3, ventpipe 4, incubator, electric machine controller 5, motor 6, reduction box 7 and temperature regulator 18, the top of incubator is provided with charging opening 12, the bottom of incubator is provided with drain hole 13, with convenient reinforced and blowing, sterile air adds pipe 2 and steam adding pipe 3 is connected with ventpipe 4 respectively, motor 6 and reduction box 7 are fixed on the frame 1, motor 6 is connected with reduction box 7, motor 6 is connected with electric machine controller 5 by lead, so that rotate and reverse by electric machine controller 5 control motors 6, it is characterized in that: described incubator is made of outer case 10 and internal box 11, outer case 10 is sheathed and be fixed on the outside of internal box 11, be water storage cavity 15 between outer case 10 and the internal box 11, fill water in the water storage cavity 15, be provided with heating rod 14 at water storage cavity 15 internal fixing, the inner chamber of internal box 11 is a culture chamber 17, charging opening 12 and drain hole 13 are connected with culture chamber 17 respectively, on the inwall of culture chamber 17, be provided with temperature-sensing probe 16, heating rod 14 is connected with temperature regulator 18 by lead respectively with temperature-sensing probe 16, like this when the temperature in the culture chamber 17 that temperature-sensing probe 16 detects is not suitable for culture temperature, temperature regulator 18 can be controlled heating rod 14 work, water in the water storage cavity 15 is heated, by water temperature culture chamber 17 is heated, when the temperature in the culture chamber 17 reaches requirement, temperature regulator 18 stops heating rod 14 work, so circulation, guarantee that the temperature in the culture chamber 17 reaches constant, on frame 1, be arranged with two positioning seats 9, the rotating shaft of the both sides outer wall of outer case 10 respectively by bearing fixing in two positioning seats 9, whole like this incubator can rotate on positioning seat 9, so that the material in the culture chamber 17 is stirred, the drive shaft 8 of reduction box 7 is fixedlyed connected with a rotating shaft on the outer case 10, thereby drive outer case 10 by drive shaft 8 and do the forward or reverse motion, the end of ventpipe 4 enters in the culture chamber 17 after passing another rotating shaft of outer case 10, thereby steam and sterile air are transported in the culture chamber 17, during use, incubator turns around earlier clockwise, turn around counterclockwise again, thereby make culture chamber 17 interior spores reach the purpose of loose wheat bran.
Described outer case 10 and internal box 11 are the circular spheroid of stainless steel.
All be provided with cock on described charging opening 12 and the drain hole 13, to guarantee after feeding in raw material, material being cultivated at culture chamber 17 inner sealings.
Described temperature regulator 18 is a prior art, fixedly is located on the outer wall of outer case 10; Described electric machine controller 5 also is a prior art, fixedly is located on the frame 1, as long as the rotating speed of control incubator is 0-5RPM, and adjustable getting final product, turn to turning around clockwise and turn around counterclockwise again, so seldom state.
Before the use, add 3 pairs of culture chambers 17 of pipe by steam earlier and carry out steam sterilizing.
Claims (4)
1. automatic rotary microbial spore incubator, comprise frame (1), sterile air adds pipe (2), steam adds pipe (3), ventpipe (4), incubator, electric machine controller (5), motor (6), reduction box (7) and temperature regulator (18), the top of incubator is provided with charging opening (12), the bottom of incubator is provided with drain hole (13), sterile air adds pipe (2) and steam adding pipe (3) is connected with ventpipe (4) respectively, motor (6) and reduction box (7) are fixed on the frame (1), motor (6) is connected with reduction box (7), motor (6) is connected with electric machine controller (5) by lead, it is characterized in that: described incubator is made of outer case (10) and internal box (11), outer case (10) is sheathed and be fixed on the outside of internal box (11), be water storage cavity (15) between outer case (10) and the internal box (11), water storage cavity fills water in (15), be provided with heating rod (14) at water storage cavity (15) internal fixing, the inner chamber of internal box (11) is culture chamber (17), charging opening (12) and drain hole (13) are connected with culture chamber (17) respectively, on the inwall of culture chamber (17), be provided with temperature-sensing probe (16), heating rod (14) is connected with temperature regulator (18) by lead respectively with temperature-sensing probe (16), on frame (1), be arranged with two positioning seats (9), the rotating shaft of the both sides outer wall of outer case (10) respectively by bearing fixing in two positioning seats (9), the drive shaft (8) of reduction box (7) is fixedlyed connected with a rotating shaft on the outer case (10), and the end of ventpipe (4) enters in the culture chamber (17) after passing another rotating shaft of outer case (10).
2. automatic rotary microbial spore incubator according to claim 1 is characterized in that: described outer case (10) and internal box (11) are the circular spheroid of stainless steel.
3. automatic rotary microbial spore incubator according to claim 1 is characterized in that: all be provided with cock on described charging opening (12) and the drain hole (13).
4. automatic rotary microbial spore incubator according to claim 1 is characterized in that: described temperature regulator (18) fixedly is located on the outer wall of outer case (10); Described electric machine controller (5) fixedly is located on the frame (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100679288A CN102212472A (en) | 2011-03-22 | 2011-03-22 | Automatic rotary microbial spore incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100679288A CN102212472A (en) | 2011-03-22 | 2011-03-22 | Automatic rotary microbial spore incubator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102212472A true CN102212472A (en) | 2011-10-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100679288A Pending CN102212472A (en) | 2011-03-22 | 2011-03-22 | Automatic rotary microbial spore incubator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102212472A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015066954A1 (en) * | 2013-11-07 | 2015-05-14 | 中国科学院天津工业生物技术研究所 | Solid-state biological reaction device and method for preparing filamentous fungus spores by using same |
| CN108865872A (en) * | 2018-09-30 | 2018-11-23 | 安徽固德生物工程有限公司 | Wheat bran bottle |
| CN110257246A (en) * | 2019-07-19 | 2019-09-20 | 深圳赛动生物自动化有限公司 | A kind of smooth shell type carbon dioxide incubator and its working method |
| CN111575145A (en) * | 2020-04-15 | 2020-08-25 | 亿利资源集团有限公司 | Fodder microbial fermentation device |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2082269U (en) * | 1991-03-13 | 1991-08-07 | 门田生 | Multi-function microbe solid fermentation machine drived by steam or electricity |
| CN2880836Y (en) * | 2005-10-31 | 2007-03-21 | 陕西科技大学 | A kind of cellulite bacteria reactor |
| CN201280554Y (en) * | 2008-10-28 | 2009-07-29 | 苏延友 | Miniature multi-use fungus liquid fermentation tank |
| CN201999940U (en) * | 2011-03-22 | 2011-10-05 | 莱芜泰禾生化有限公司 | Automatic rotary microorganism spore culture device |
-
2011
- 2011-03-22 CN CN2011100679288A patent/CN102212472A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2082269U (en) * | 1991-03-13 | 1991-08-07 | 门田生 | Multi-function microbe solid fermentation machine drived by steam or electricity |
| CN2880836Y (en) * | 2005-10-31 | 2007-03-21 | 陕西科技大学 | A kind of cellulite bacteria reactor |
| CN201280554Y (en) * | 2008-10-28 | 2009-07-29 | 苏延友 | Miniature multi-use fungus liquid fermentation tank |
| CN201999940U (en) * | 2011-03-22 | 2011-10-05 | 莱芜泰禾生化有限公司 | Automatic rotary microorganism spore culture device |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015066954A1 (en) * | 2013-11-07 | 2015-05-14 | 中国科学院天津工业生物技术研究所 | Solid-state biological reaction device and method for preparing filamentous fungus spores by using same |
| CN108865872A (en) * | 2018-09-30 | 2018-11-23 | 安徽固德生物工程有限公司 | Wheat bran bottle |
| CN110257246A (en) * | 2019-07-19 | 2019-09-20 | 深圳赛动生物自动化有限公司 | A kind of smooth shell type carbon dioxide incubator and its working method |
| CN111575145A (en) * | 2020-04-15 | 2020-08-25 | 亿利资源集团有限公司 | Fodder microbial fermentation device |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20111012 |