CN102204536A - Antibacterial composition containing picoxystrobin and antibiotic compound - Google Patents

Antibacterial composition containing picoxystrobin and antibiotic compound Download PDF

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Publication number
CN102204536A
CN102204536A CN2011100955964A CN201110095596A CN102204536A CN 102204536 A CN102204536 A CN 102204536A CN 2011100955964 A CN2011100955964 A CN 2011100955964A CN 201110095596 A CN201110095596 A CN 201110095596A CN 102204536 A CN102204536 A CN 102204536A
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zen
active component
bactericidal composition
polyoxin
percentage
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CN2011100955964A
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Chinese (zh)
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张伟
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陕西汤普森生物科技有限公司
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Publication of CN102204536A publication Critical patent/CN102204536A/en

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Abstract

The invention discloses an antibacterial composition containing picoxystrobin and an antibiotic compound, which comprises an active component A and an active component B, wherein the active component A may be picoxystrobin; the active component B may be any of the following antibacterial agent: validamycin, Zhongsheng mycin, kasugamycin and polyoxin; and the weight percentage ratio of the active component A to the active component B is (1-60 percent):(1-40 percent). The composition can prevent and control various diseases in crops, has an obvious synergetic effect, expands an antibacterial spectrum and has high activities for epidemic disease, downy mildew, banded sclerotial blight, rice blast, ring rot, powdery mildew, gray mold, leaf mold, blight and alternaria leaf spot; and the dose of pesticide is reduced, the residual pesticide on crops is reduced, and environment pollution is relieved.

Description

A kind of bactericidal composition that contains ZEN 90160 and antibiotics compound

Technical field

The invention belongs to technical field of pesticide, relate to a kind of bactericidal composition that contains ZEN 90160 and antibiotics compound.

Background technology

ZEN 90160, English name: fenbuconazole, molecular formula: C 19H 17ClN 4, chemical name: 4-(4-chlorphenyl)-2-phenyl-2 (1H-1,2,4-triazol-1-yl methyl) butyronitrile, structural formula:

ZEN 90160 sterol demethylation inhibitor, the inner sucting conduction type bactericide can suppress the elongation of pathogen mycelia, stops the germ spore that has germinateed to be invaded and makes fabric texture.Use latent period germ, can stop the growth of germ, use after morbidity, can make spore distortion of future generation, lose and continue the infection ability, existing thick anti-effect has therapeutic action again to disease.

The bioactivator that the secondary metabolite that antibiotics bactericide origin takes place in microorganism reaches to take place is a model, carries out synthetic or structure and refreshes, and becomes artificial semisynthetic product.Inhale in this class antibiotic has function, efficient, selectivity is strong, have that treatment and care are contaminated, biological degradation is fast, nuisanceless, to interests such as people and animals' safeties.

Yet in the real process of agricultural production, preventing and treating the problem that disease is easy to generate most is the drug-fast generation of germ.The different cultivars composition carries out composite, is to prevent and treat the very common method of resistance germ.Heterogeneity is carried out composite, according to practical application effect, judge certain composite be synergy, add and or antagonism.In most cases, the composite effect of agricultural chemicals all is an additive effect, and the seldom composite of synergistic effect really arranged, especially synergistic effect very obviously, synergy ratio very high composite just still less.Through inventor's research, discovery can produce good synergistic effect with ZEN 90160 and jinggangmeisu, middle living rhzomorph, kasugarnycin, polyoxin after composite, and still unexposed about the composite relevant report of ZEN 90160 and jinggangmeisu, middle living rhzomorph, kasugarnycin, polyoxin.

Summary of the invention

A kind of bactericidal composition that contains ZEN 90160 and antibiotics compound, comprise effective active component, auxiliary agent and filler, it is characterized in that: active component A, B percentage by weight are 1~60%: 1~40%, described active component A is selected from ZEN 90160, and active component B is selected from a kind of in jinggangmeisu, middle living rhzomorph, kasugarnycin, the polyoxin.

Described a kind of bactericidal composition that contains ZEN 90160 and antibiotics compound is characterized in that: the percentage by weight of A, two kinds of active components of B is 1~50%: 1~30%.

The described bactericidal composition that contains ZEN 90160 is characterized in that: the percentage by weight of ZEN 90160 and jinggangmeisu is 5~40%: 5~25%.

The described bactericidal composition that contains ZEN 90160 is characterized in that: the percentage by weight of ZEN 90160 and middle living rhzomorph is 5~40%: 1~20%.

The described bactericidal composition that contains ZEN 90160 is characterized in that: the percentage by weight of ZEN 90160 and kasugarnycin is 5~40%: 1~20%.

The described bactericidal composition that contains ZEN 90160 is characterized in that: the percentage by weight of ZEN 90160 and polyoxin is 5~40%: 5~25%.

The percentage by weight of A, two kinds of active components of B is 1~60%: 1~40%.Usually active weight percentage is 0.5~90% of a gross weight in the composition, and preferable is 5%~85%.According to different preparation types, the active component content scope is different.Usually, liquid preparation contains 1~70% active substance by weight, preferably is 5~50%; Solid pharmaceutical preparation contains 5~85% active substance by weight, preferably is 10~80%.

At least contain a kind of surfactant in the bactericidal composition of the present invention, the dispersion of active component in water when being beneficial to use.Surface-active contents is 5~30% of a total formulation weight amount, and surplus is solid or liquid diluent.

The selected surfactant of bactericidal composition of the present invention is known in those skilled in the art: can be selected from dispersant, wetting agent, binding agent or the defoamer one or more.According to different dosage form, can also contain disintegrant known in those skilled in the art, antifreeze etc. in the preparation.

Described dispersant is selected from alkylnaphthalene sulfonate, two (alkyl) naphthalenesulfonate formaldehyde condensation compound, naphthalene sulfonic acid-formaldehyde condensation product, aryl phenol polyoxyethylene succinate sulfonate, octylphenol polyethylene oxyethylene group ether sulfate, polycarboxylate, lignosulfonates, the alkylaryl APEO, aliphatic alcohol polyethenoxy base ether, the phonetic formaldehyde condensation products sulphate of alkylphenol-polyethenoxy, alkyl benzene sulfonate calcium salt, the naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkylphenol-polyethenoxy is phonetic, aliphatic amine polyoxyethylene is phonetic, polyoxyethylene carboxylate, during the ester polyoxyethylene is phonetic one or more.

Described wetting agent is selected from: one or more in lauryl sodium sulfate, neopelex, Nekal BX, wetting and penetrating agent F, spaonin powder, silkworm excrement, the soapberry powder.

Described disintegrant is selected from: one or more in bentonite, urea, ammonium sulfate, the aluminium chloride.

Described binding agent is selected from: one or more in gum Arabic, xanthans, sodium phosphate trimer, phenolic resins, sodium alginate, white dextrin, methylcellulose, PAA, polyvinylpyrrolidone, crospolyvinylpyrrolidone, polyethylene glycol, the polyvinyl alcohol.

Described antifreeze is selected from: one or more in ethylene glycol, propane diols, glycerine, the polyethylene glycol.

Described defoamer is selected from: silicone, C 8~10Aliphatic alcohols, C 10~20In saturated fat acids (as capric acid) and acid amides, silicone oil, the silicone compound one or more.

Described emulsifier is selected from: agricultural newborn 500# (alkyl benzene calcium sulfonate), OP series phosphate (polyoxyethylene nonylphenol ether phosphate), 600# phosphate (phenyl phenol polyoxy ethyl ether phosphate), styrene polyoxyethylene ether ammonium sulfate salt, alkyl biphenyl ether disulfonic acid magnesium salts, triethanolamine salt, farming newborn 400# (benzyl dimethyl phenol polyoxy ethyl ether), farming newborn 600# (phenyl phenol polyoxy ethyl ether), Nongru-700 # (alkylphenol-formaldehyde resin polyoxy ethyl ether), farming newborn 36# (phenethyl fluosite polyoxy ethyl ether), farming newborn 1600# (phenethyl phenol polyoxy ethyl polypropylene-base ether), ethylene oxide-propylene oxide block copolymer, OP series (polyoxyethylene nonylphenol ether), By series (castor oil polyoxyethylene ether), farming newborn 33# (alkylaryl polyoxyethylene polyoxypropylene ether), farming newborn 34# (alkylaryl polyoxyethylene poly-oxygen propylene aether), sapn series (sorbitan monostearate), tween series (sorbitan fatty acid ester APEO), in the AEO series (the pure APEO of fat) one or more.

Described stabilizing agent is selected from one or more in sodium sorbate, butyl glycidyl ether, phenyl glycidyl ether, cresyl glycidyl ether, the polyethylene glycol diglycidyl ether;

Described filler is selected from: one or more in kaolin, diatomite, bentonite, attapulgite, white carbon, starch, the precipitated calcium carbonate.

Composition of the present invention can be by the user before use through dilution or directly use.Its preparation can be by the preparation of common processing method, is about to active substance and after liquid flux or solid carrier mix, and adds in surfactant such as dispersant, stabilizing agent, wetting agent, adhesive, the defoamer etc. one or more again.

Bactericidal composition of the present invention can be processed into acceptable forms on any agricultural chemicals on demand.Wherein preferred dosage form is wetting powder, water dispersible granules, suspending agent, suspension emulsion.

When making wetting powder, composition comprises following constituent content: ZEN 90160 1~60%, active component B 1~40%, dispersant 5~10%, wetting agent 2~10%, filler 8~91%.

Active component ZEN 90160, active component B, dispersant, wetting agent, filler are mixed, in mixed cylinder, mix, after airslide disintegrating mill is pulverized, mix again, promptly can be made into the wetting powder product of the present composition.

When making water dispersible granules, composition comprises following constituent content: active component ZEN 90160 1~60%, active component B 1~40%, dispersant 3~12%, wetting agent 1~8%, disintegrant 1~10%, binding agent 1~8%, filler 10~92%.

Active component ZEN 90160, active component B, dispersant, wetting agent, disintegrant, filler etc. are obtained the particle diameter that needs through comminution by gas stream together, add other auxiliary agents such as binding agent again, obtain the materials of granulating.It is interior through after granulation and the drying to expect that product are quantitatively sent the fluidized bed granulation dryer to, makes the water dispersible granules product of the present composition.

Comprise following constituent content when composition is made suspending agent: active component ZEN 90160 1~40%, active component B 1~20%, dispersant 2~10%, wetting agent 2~10%, defoamer 0.1~1%, binding agent 0.1~2%, antifreeze 0.1~8%, deionized water add to 100%.

Dispersant, wetting agent, defoamer, thickener, antifreeze in the above-mentioned formulation material are mixed through high speed shear, add active component ZEN 90160, active component B, ball milling is 2~3 hours in ball mill, make diameter of particle all below 5 μ m, make the suspending agent product of the present composition.

Comprise following constituent content when composition is made suspension emulsion: ZEN 90160 1~40%, active component B 1~20%, emulsifier 2~12%, dispersant 2~10%, defoamer 0.1~2%, thickener 0.1~2%, antifreeze 0.1~8%, stabilizing agent 0.05~3%, water add to 100%.

Dispersant, defoamer, thickener, antifreeze, stabilizing agent in the above-mentioned formulation material are mixed through high speed shear, add the former medicine of effective active composition B, ball milling is 2~3 hours in ball mill, make diameter of particle all below 5 μ m, make effective active composition B suspending agent, then with the former medicine of ZEN 90160, emulsifier and various auxiliary agent with the direct emulsification of high speed agitator in suspending agent, make the suspension emulsion product of the present composition.

The invention has the advantages that: after (1) ZEN 90160 and active component B are composite, have obviously lasting synergistic effect; (2) enlarged fungicidal spectrum, eqpidemic disease, downy mildew, banded sclerotial blight, rice blast, ring spot, powdery mildew, gray mold, leaf mold, fusarium wilt, spot defoliation disease have all been had greater activity; (3) the present invention not with an organic solvent is not difficult for producing poisoning, is convenient to transportation and storage; (4) reduce the dosage of agricultural chemicals, reduced the residual quantity of agricultural chemicals on crop, alleviated environmental pollution.

Embodiment

To further instruction of the present invention, the percentage among the embodiment all is weight percentage below in conjunction with embodiment, but the present invention is not limited thereto.

The embodiment of the invention is the method that adopts Toxicity Determination and field trial to combine.

Application Example one

Example 1 50% ZEN 90160 jinggangmeisu wetting powder

ZEN 90160 40%, jinggangmeisu 10%, polyoxyethylene carboxylate 8%, spaonin powder 6%, diatomite add to 100%, and mixture carries out comminution by gas stream, make 50% ZEN 90160 jinggangmeisu wetting powder.

Example 2 75% ZEN 90160 jinggangmeisu wetting powders

ZEN 90160 50%, jinggangmeisu 25%, aryl phenol polyoxyethylene succinate sulfonate 6%, lauryl sodium sulfate 4%, attapulgite add to 100%, and mixture carries out comminution by gas stream, make 75% ZEN 90160 jinggangmeisu wetting powder.

Example 3 45% ZEN 90160 jinggangmeisu water dispersible granules

ZEN 90160 40%, jinggangmeisu 5%, alkylphenol-polyethenoxy are phonetic 7%, wetting and penetrating agent F 5%, urea 1.9%, bentonite add to 100%, mixed 45% ZEN 90160 jinggangmeisu water dispersible granules.

Example 4 65% ZEN 90160 jinggangmeisu water dispersible granules

ZEN 90160 50%, jinggangmeisu 15%, alkylaryl APEO 5%, silkworm excrement 5%, ammonium sulfate 2%, white carbon add to 100%, the mixed 65% ZEN 90160 jinggangmeisu water dispersible granules that gets.

Example 5 15% ZEN 90160 jinggangmeisu suspending agents

ZEN 90160 10%, jinggangmeisu 5%, naphthalene sulfonic acid-formaldehyde condensation product 8%, lauryl sodium sulfate 2%, C 8~10Aliphatic alcohols 0.4%, PAA 1.7%, glycerine 1.1%, deionized water add to 100%, the mixed 15% ZEN 90160 jinggangmeisu suspending agent that gets.

Example 6 30% ZEN 90160 jinggangmeisu suspending agents

ZEN 90160 20%, jinggangmeisu 10%, polycarboxylate 5%, soapberry powder 3%, C 10~20Saturated fat acids 0.1%, white dextrin 1.8%, ethylene glycol 1.9%, deionized water add to 100%, the mixed 30% ZEN 90160 jinggangmeisu suspending agent that gets.

Example 7 10% ZEN 90160 jinggangmeisu suspension emulsions

ZEN 90160 5%, jinggangmeisu 5%, lignosulfonates 4%, spaonin powder 3%, silicone oil 0.3%, sodium alginate 1.2%, agricultural newborn 500#8%, diethylene glycol (DEG) 1.6%, water add to 100%, the aforementioned formula material is mixed, promptly make 10% ZEN 90160 jinggangmeisu suspension emulsion.

Example 8 36% ZEN 90160 jinggangmeisu suspension emulsions

ZEN 90160 30%, jinggangmeisu 6%, alkyl benzene sulfonate calcium salt 7%, wetting and penetrating agent F 2%, agricultural newborn 400#6%, xanthans 1.3%, propane diols 1.7%, silicone compound 0.2%, water add to 100%, through the mixed 36% ZEN 90160 jinggangmeisu suspension emulsion that gets.

Give birth to the rhzomorph wetting powder in example 9 44% ZEN 90160

ZEN 90160 40%, middle living rhzomorph 4%, ester polyoxyethylene are phonetic 7%, neopelex 6%, bentonite add to 100%, and mixture carries out comminution by gas stream, makes and gives birth to the rhzomorph wetting powder in 44% ZEN 90160.

Give birth to the rhzomorph wetting powder in example 10 60% ZEN 90160

ZEN 90160 50%, middle living rhzomorph 10%, two (alkyl) naphthalenesulfonate formaldehyde condensation compound 5%, tea are withered 3%, white carbon adds to 100%, and mixture carries out comminution by gas stream, makes and gives birth to the rhzomorph wetting powder in 60% ZEN 90160.

Give birth to the rhzomorph water dispersible granules in example 11 48% ZEN 90160

ZEN 90160 45%, middle living rhzomorph 3%, aliphatic amine polyoxyethylene are phonetic 8%, Nekal BX 6%, aluminium chloride 1.8%, attapulgite add to 100%, give birth to the rhzomorph water dispersible granules in mixed 48% ZEN 90160.

Give birth to the rhzomorph water dispersible granules in example 12 50% ZEN 90160

ZEN 90160 45%, middle living rhzomorph 5%, octylphenol polyethylene oxyethylene group ether sulfate 6%, silkworm excrement 5%, bentonite add to 100%, and mixed getting given birth to the rhzomorph water dispersible granules in 50% ZEN 90160.

Give birth to the rhzomorph suspending agent in example 13 22% ZEN 90160

ZEN 90160 20%, middle living rhzomorph 2%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 6%, wetting and penetrating agent F 3%, silicone compound 0.2%, polyvinylpyrrolidone 1.7%, triethylene glycol 1.4%, deionized water add to 100%, and mixed getting given birth to the rhzomorph suspending agent in 22% ZEN 90160.

Give birth to the rhzomorph suspending agent in example 14 36% ZEN 90160

ZEN 90160 30%, middle living rhzomorph 6%, aliphatic alcohol polyethenoxy base ether 7%, soapberry powder 1%, C 8~10Aliphatic alcohols 0.5%, phenolic resins 1.1%, polyethylene glycol 1.9%, deionized water add to 100%, and mixed getting given birth to the rhzomorph suspending agent in 36% ZEN 90160.

Give birth to the rhzomorph suspension emulsion in example 15 10% ZEN 90160

ZEN 90160 5%, middle living rhzomorph 5%, polyoxyethylene carboxylate 5%, lauryl sodium sulfate 3%, silicone oil 0.2%, sodium phosphate trimer 1.3%, diethylene glycol (DEG) 1.8%, 600# phosphate 6%, water add to 100%, the aforementioned formula material is mixed, promptly make and give birth to the rhzomorph suspension emulsion in 10% ZEN 90160.

Give birth to the rhzomorph suspension emulsion in example 16 24% ZEN 90160

ZEN 90160 20%, middle living rhzomorph 4%, naphthalene sulfonic acid-formaldehyde condensation product 8%, soapberry powder 2%, methylcellulose 1.8%, glycerine 1.5%, C 10~20Saturated fat acids 0.1%, agricultural newborn 1600#7%, water add to 100%, give birth to the rhzomorph suspension emulsion in 24% ZEN 90160 through mixed getting.

Example 17 33% ZEN 90160 kasugarnycin wetting powders

ZEN 90160 30%, kasugarnycin 3%, alkylphenol-polyethenoxy are phonetic 6%, Nekal BX 6%, bentonite add to 100%, and mixture carries out comminution by gas stream, makes 33% ZEN 90160 kasugarnycin wetting powder.

Example 18 48% ZEN 90160 kasugarnycin wetting powders

ZEN 90160 40%, kasugarnycin 8%, alkylnaphthalene sulfonate 7%, spaonin powder 5%, diatomite add to 100%, and mixture carries out comminution by gas stream, make 48% ZEN 90160 kasugarnycin wetting powder.

Example 19 32% ZEN 90160 kasugamycin water-dispersion granules

ZEN 90160 30%, kasugarnycin 2%, alkylaryl APEO 6%, wetting and penetrating agent F 4%, sodium carbonate 2%, kaolin add to 100%, the mixed 32% ZEN 90160 kasugamycin water-dispersion granule that gets.

Example 20 55% ZEN 90160 kasugamycin water-dispersion granules

ZEN 90160 50%, kasugarnycin 5%, alkyl benzene sulfonate calcium salt 7%, lauryl sodium sulfate 6%, ammonium sulfate 2.5%, bentonite add to 100%, the mixed 55% ZEN 90160 kasugamycin water-dispersion granule that gets.

Example 21 30% ZEN 90160 kasugarnycin suspending agents

ZEN 90160 25%, kasugarnycin 5%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 6%, spaonin powder 2%, silicone compound 0.1%, methylcellulose 1.4%, triethylene glycol 1.9%, deionized water add to 100%, the mixed 30% ZEN 90160 kasugarnycin suspending agent that gets.

Example 22 32% ZEN 90160 kasugarnycin suspending agents

ZEN 90160 30%, kasugarnycin 2%, polyoxyethylene carboxylate 5%, neopelex 2%, silicone oil 0.2%, gum Arabic 1.1%, ethylene glycol 1.7%, deionized water add to 100%, the mixed 32% ZEN 90160 kasugarnycin suspending agent that gets.

Example 23 6% ZEN 90160 kasugarnycin suspension emulsions

ZEN 90160 3%, kasugarnycin 3%, lignosulfonates 8%, lauryl sodium sulfate 3%, silicone oil 0.6%, propane diols 1.3%, white dextrin 1.6%, agricultural newborn 600#8%, water adds to 100%, the mixed 6% ZEN 90160 kasugarnycin suspension emulsion that gets.

Example 24 30% ZEN 90160 kasugarnycin suspension emulsions

ZEN 90160 28%, kasugarnycin 2%, the phonetic formaldehyde condensation products sulphate 8% of alkylphenol-polyethenoxy, spaonin powder 2%, PAA 1.3%, tween series 6%, C 10~20Saturated fat acids 0.4%, polyethylene glycol 1.5%, water add to 100%, the mixed 30% ZEN 90160 kasugarnycin suspension emulsion that gets.

Example 25 45% ZEN 90160 polyoxin wetting powders

ZEN 90160 40%, polyoxin 5%, lignosulfonates 8%, neopelex 7%, kaolin add to 100%, and mixture carries out comminution by gas stream, make 45% ZEN 90160 polyoxin wetting powder.

Example 26 60% ZEN 90160 polyoxin wetting powders

ZEN 90160 50%, polyoxin 10%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 6%, Nekal BX 4%, attapulgite add to 100%, and mixture carries out comminution by gas stream, make 60% ZEN 90160 polyoxin wetting powder.

Example 27 50% ZEN 90160 polyoxin water dispersible granules

ZEN 90160 40%, polyoxin 10%, alkylphenol-polyethenoxy are phonetic 7%, wetting and penetrating agent F 6%, sodium bicarbonate 1.6%, bentonite add to 100%, mixed 50% ZEN 90160 polyoxin water dispersible granules.

Example 28 60% ZEN 90160 polyoxin water dispersible granules

ZEN 90160 40%, polyoxin 20%, two (alkyl) naphthalenesulfonate formaldehyde condensation compound 5%, spaonin powder 5%, urea 2.3%, diatomite add to 100%, the mixed 60% ZEN 90160 polyoxin water dispersible granules that gets.

Example 29 20% ZEN 90160 polyoxin suspending agents

ZEN 90160 10%, polyoxin 10%, ester polyoxyethylene are phonetic 8%, lauryl sodium sulfate 1%, C 8~10Aliphatic alcohols 0.2%, xanthans 1.7%, glycerine 2%, deionized water add to 100%, the mixed 20% ZEN 90160 polyoxin suspending agent that gets.

Example 30 45% ZEN 90160 polyoxin suspending agents

ZEN 90160 30%, polyoxin 15%, poly-hydroxy acid salt 6%, neopelex 2%, silicone compound 0.3%, polyvinylpyrrolidone 1.6%, diethylene glycol (DEG) 1.8%, deionized water add to 100%, the mixed 45% ZEN 90160 polyoxin suspending agent that gets.

Example 31 15% ZEN 90160 polyoxin suspension emulsions

ZEN 90160 5%, polyoxin 10%, alkyl benzene sulfonate calcium salt 6%, wetting and penetrating agent F 4%, silicone compound 0.5%, ethylene glycol 1.2%, polyvinylpyrrolidone 1.7%, OP series phosphate 5%, water adds to 100%, the mixed 15% ZEN 90160 polyoxin suspension emulsion that gets.

Example 32 36% ZEN 90160 polyoxin suspension emulsions

ZEN 90160 24%, polyoxin 12%, lignosulfonates 5%, tea are withered 3%, gum Arabic 1.1%, agricultural newborn 33#7%, C 10~20Saturated fat acids 0.3%, propane diols 1.8%, water add to 100%, the mixed 36% ZEN 90160 polyoxin suspension emulsion that gets.

Wetting powder the key technical indexes of the present invention:

Table 1

Water dispersible granules the key technical indexes of the present invention:

Table 2

Suspending agent the key technical indexes of the present invention:

Table 3

Suspension emulsion the key technical indexes of the present invention:

Table 4

The embodiment of the invention is the method that adopts Toxicity Determination and field trial to combine.The embodiment of the invention is the method that adopts Toxicity Determination and field trial to combine.Pass through Toxicity Determination earlier, the synergy ratio (SR) after clear and definite two kinds of medicaments are composite by a certain percentage, SR<0.5 is an antagonism, and 0.5≤SR≤1.5 are summation action, and SR>1.5 are synergistic effect, on this basis, carry out field trial again.

ZEN 90160 and active component B (B is selected from a kind of of jinggangmeisu, middle living rhzomorph, kasugarnycin, polyoxin) are composite to the crop pest Toxicity Determination.

After the effective inhibition concentration scope of each medicament was determined in prerun, each medicament was established 5 dosage respectively by active constituent content and is handled, and established the clear water contrast.Carry out with reference to " agricultural chemicals indoor bioassay test rule bactericide ", adopt the mycelial growth rate method to measure the virulence of medicament the crop germ.Measure colony diameter with the right-angled intersection method behind the 72h, calculate and respectively handle clean amount of growth, mycelial growth inhibition rate.

Clean amount of growth (mm)=measurement colony diameter-5

Mycelial growth inhibition rate is converted into probit value (y), and liquor strength (μ g/mL) converts logarithm value (x) to, tries to achieve virulence regression equation (y=a+bx) with method of least squares, and calculates the EC of every kind of medicament thus 50Value.Calculate the different proportioning synergy ratios (SR) of two medicaments according to the Wadley method simultaneously, SR<0.5 is an antagonism, and 0.5≤SR≤1.5 are summation action, and SR>1.5 are synergistic effect.Computing formula is as follows:

Wherein: a, b are respectively active component ZEN 90160 and active component B (jinggangmeisu, middle living rhzomorph, kasugarnycin, polyoxin) shared ratio in combination;

A is a ZEN 90160;

B is a kind of in jinggangmeisu, middle living rhzomorph, kasugarnycin, the polyoxin.

Implementation of an application example two ZEN 90160 and composite jinggangmycin are to the rice sheath blight disease Toxicity Determination.

Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..

Experimental scheme: effective inhibition concentration scope of determining ZEN 90160, the former medicine of jinggangmeisu and the two different proportioning mixture through preliminary experiment.

Toxicity test result

Table 5 ZEN 90160, jinggangmeisu and composite toxicity test analysis of results table thereof to rice sheath blight disease

As shown in Table 5, ZEN 90160, jinggangmeisu are to the EC of rice sheath blight disease 50Be respectively 5.46mg/L and 2.24mg/L.ZEN 90160 and jinggangmeisu proportioning were at 60: 1 to 1: 40 o'clock, synergy ratio SR is all greater than 1.5, illustrating that ZEN 90160 and jinggangmeisu were mixed in the scope at 60: 1 to 1: 40 all shows synergistic effect, especially when the two proportioning during at 8: 1 to 1: 2, synergistic effect is more obviously outstanding, synergy ratio SR maximum when ZEN 90160 and jinggangmeisu weight ratio are 2: 1 wherein, synergistic effect is the most obvious.

Implementation of an application example three ZEN 90160 and middle living rhzomorph are composite to the alternaria leaf spot of apple Toxicity Determination.

Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..

Experimental scheme: effective inhibition concentration scope of determining ZEN 90160, the former medicine of middle living rhzomorph and the two different proportioning mixture through preliminary experiment.

Toxicity test result

Table 6 ZEN 90160, middle living rhzomorph and composite toxicity test analysis of results table thereof to alternaria leaf spot of apple

As shown in Table 6, ZEN 90160, middle living rhzomorph are to the EC of alternaria leaf spot of apple 50Be respectively 5.02mg/L and 0.83mg/L.ZEN 90160 and middle living rhzomorph proportioning were at 60: 1 to 1: 40 o'clock, synergy ratio SR is all greater than 1.5, illustrating that ZEN 90160 and middle living rhzomorph were mixed in the scope at 60: 1 to 1: 40 all shows synergistic effect, especially when the two proportioning during at 15: 1 to 1: 5, synergistic effect is more obviously outstanding, synergy ratio SR maximum when ZEN 90160 and middle living rhzomorph weight ratio are 5: 1 wherein, synergistic effect is the most obvious.

Implementation of an application example four: ZEN 90160 and kasugarnycin are composite to the rice blast Toxicity Determination.

Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..

Experimental scheme: effective inhibition concentration scope of determining ZEN 90160, the former medicine of kasugarnycin and the two different proportioning mixture through preliminary experiment.

Toxicity test result

Table 7 ZEN 90160, kasugarnycin and composite toxicity test analysis of results table thereof to rice blast

As shown in Table 7, ZEN 90160, kasugarnycin are to the EC of rice blast 50Be respectively 4.98mg/L and 0.76mg/L.ZEN 90160 compares at 60: 1 to 1: 40 o'clock with kasugarnycin, synergy ratio SR is all greater than 1.5, illustrating that ZEN 90160 and kasugarnycin were mixed in the scope at 60: 1 to 1: 40 all shows synergistic effect, especially when the two proportioning during at 15: 1 to 1: 5, synergistic effect is more obviously outstanding, synergy ratio SR maximum when ZEN 90160 and kasugarnycin weight ratio are 5: 1 wherein, synergistic effect is the most obvious.

Implementation of an application example five: ZEN 90160 and polyoxin are composite to the wheat powdery mildew Toxicity Determination.

Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..

Experimental scheme: effective inhibition concentration scope of determining ZEN 90160, the former medicine of polyoxin and the two different proportioning mixture through preliminary experiment.

Toxicity test result

Table 8 ZEN 90160, polyoxin and composite toxicity test analysis of results table thereof to wheat powdery mildew

As shown in Table 8, ZEN 90160, polyoxin are to the EC of wheat powdery mildew 50Be respectively 5.31mg/L and 2.43mg/L.ZEN 90160 compares at 60: 1 to 1: 40 o'clock with polyoxin, synergy ratio SR is all greater than 1.5, illustrating that ZEN 90160 and polyoxin were mixed in the scope at 60: 1 to 1: 40 all shows synergistic effect, especially when the two proportioning during at 8: 1 to 1: 2, synergistic effect is more obviously outstanding, synergy ratio SR maximum when ZEN 90160 and polyoxin weight ratio are 2: 1 wherein, synergistic effect is the most obvious.

Application Example six ZEN 90160 and jinggangmeisu and the composite rice sheath blight disease test of pesticide effectiveness of preventing and treating thereof

This experimental establishment is in the Guangxi province Nanning suburban district, and test medicine is researched and developed, provided by Shanxi Thompson Biological Technology Co., Ltd., contrast medicament 25% ZEN 90160 suspending agent (commercial), 3% jinggangmeisu aqua (commercial).

The preceding investigation of medicine rice sheath blight disease disease index, in the their early stage dispenser, dispenser for the second time after 10 days, dispenser is 2 times altogether, investigates disease indexs in 7 days, 14 days, 30 days after the dispenser for the second time and calculates preventive effect.Experimental result is as follows:

Table 9 ZEN 90160, jinggangmeisu and the composite rice sheath blight disease test of pesticide effectiveness of preventing and treating thereof

As can be seen from Table 9, can effectively prevent and treat rice sheath blight disease behind ZEN 90160 and the composite jinggangmycin, control efficiency all is better than the preventive effect of single agent.In the test scope of medication, the target crop is had no adverse effects.

Application Example seven ZEN 90160 and middle living rhzomorph and the composite control alternaria leaf spot of apple test of pesticide effectiveness thereof

This experimental establishment is at Liquan County, Xianyang, Shanxi province city footpath between fields Dong Zhen, and test medicine is researched and developed, provided by Shanxi Thompson Biological Technology Co., Ltd., gives birth to rhzomorph wetting powder (commercial) in the contrast medicament 25% ZEN 90160 suspending agent (commercial), 3%.

The investigation alternaria leaf spot of apple state of an illness before the medicine, in the dispenser for the first time of state of an illness initial stage, dispenser in per 7 days once is total to dispenser 2 times.Investigated disease index respectively and calculated preventive effect in 7 days, 14 days, 30 days after the dispenser for the second time.Experimental result is as follows:

Table 10 ZEN 90160, middle living rhzomorph and the composite control alternaria leaf spot of apple test of pesticide effectiveness thereof

As can be seen from Table 10, can effectively prevent and treat alternaria leaf spot of apple after ZEN 90160 and middle living rhzomorph are composite, control efficiency all is better than the preventive effect of single agent.In the test scope of medication, the target crop is had no adverse effects.

Application Example eight ZEN 90160 and kasugarnycin and the composite control rice blast test of pesticide effectiveness thereof

This experimental establishment is in Tuanfeng County, Huanggang City, Hubei Province, and test medicine is researched and developed, provided by Shanxi Thompson Biological Technology Co., Ltd., contrast medicament 25% ZEN 90160 suspending agent (commercial), 2% kasugarnycin aqua (commercial).

The preceding investigation of medicine rice blast disease index, in the their early stage dispenser, dispens