CN102153667A - Method for purifying green tea polysaccharides - Google Patents
Method for purifying green tea polysaccharides Download PDFInfo
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- CN102153667A CN102153667A CN 201110045110 CN201110045110A CN102153667A CN 102153667 A CN102153667 A CN 102153667A CN 201110045110 CN201110045110 CN 201110045110 CN 201110045110 A CN201110045110 A CN 201110045110A CN 102153667 A CN102153667 A CN 102153667A
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- green tea
- tea polysaccharide
- tea polysaccharides
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Abstract
The invention provides a method for purifying green tea polysaccharides, comprising the following steps: carrying out chromatography on the crude extract of the green tea polysaccharides by DEAE C-52 cellulose columns, carrying out gradient elution on the crude extract of the green tea polysaccharides by 0.1-0.5mol/L sodium chloride solution, tracking and detecting the green tea polysaccharides, collecting and merging all peak tubes, carrying out the chromatography on green tea polysaccharides by Sephadex G-100 gel columns, further purifying the green tea polysaccharides, eluting the green tea polysaccharides by 0.1mol/L sodium chloride solution, collecting and merging all peak tubes, dialyzing the green tea polysaccharides by using deionized water at low temperature for 48 hours, freezing and drying the green tea polysaccharides, and carrying out UV (ultraviolet) full-waveband scanning and infrared spectroscopy analysis on the purity and the nature of the green tea polysaccharides.
Description
Technical field: the purification process that the invention provides a kind of green tea polysaccharide.
Background technology: green tea and extract thereof have eliminates free radical, multiple biological activity such as anti-oxidant.Tea polysaccharide be from tealeaves, extract with protein bound acidic polysaccharose or acid glycoprotein together, be one of important physiologically active ingredient in the tealeaves.Chinese scholars has been carried out big quantity research to the contained polyose mixture of tealeaves, finds that tea polysaccharide has effects such as the body immunity of increasing, hypoglycemic, reducing blood-fat, minimizing atherosclerosis, anticoagulation, anti-oxidant, radioprotective.
The invention provides the method for a kind of tea polysaccharide crude extract, and its purity and character are carried out scanning of ultraviolet all wave band and Infrared spectroscopy through anionite DEAE C-52 cellulose chromatography, Sephadex G-100 gel filtration chromatography purifying tea polysaccharide.
Summary of the invention: the purification process that the objective of the invention is to provide a kind of green tea polysaccharide.
The present invention is achieved in that the tea polysaccharide crude extract through DEAE C-52 cellulose chromatography, and 0.1~0.5mol/L sodium chloride solution carries out gradient elution, and fraction collection is followed the tracks of and detected polysaccharide content, and record data are also drawn elution curve.Collect each peak pipe of merging, the long-pending dehydrated alcohol precipitation of triploid is spent the night, and centrifugal, the dissolving back is the SephadexG-100 gel filtration chromatography upward, 0.1mol/L sodium chloride solution wash-out, and fraction collection is followed the tracks of and is detected polysaccharide content, and record data are also drawn elution curve.Collect each peak pipe of merging, low temperature deionized water dialysis 48 hours, UV spectrum and Infrared spectroscopy are carried out in freeze-drying.
Description of drawings: Fig. 1 tea polysaccharide is through the elution curve of DEAE C-52 cellulose column
The Sephadex G-100 gel filtration chromatography elution curve of Fig. 2 tea polysaccharide
The ultraviolet all wave band scintigram of Fig. 3 tea polysaccharide
The infrared spectrogram of Fig. 4 tea polysaccharide
Embodiment: get 100mg tea polysaccharide crude extract, be dissolved in the 10mL 0.1mol/L sodium chloride solution, and ultrasonic 25min (35 ℃, 80W), 4 ℃ of frozen centrifugation 20min of 10000rpm, DEAE C-52 cellulose column carries out gradient elution with 0.1~0.5mol/L sodium chloride solution on the settled solution that obtains, and eluent flow rate is 60mL/h, fraction collection, every pipe 6mL, sulfuric acid-phynol method follow the tracks of and detect (490nm), and record data are also drawn elution curve.Collect and merge each peak pipe, the long-pending dehydrated alcohol precipitation of triploid is spent the night, centrifugal, after dissolving fully with 15mL 0.1mol/L sodium chloride solution, get supernatant liquor 5mL and go up the SephadexG-100 gel column, 0.1mol/L sodium chloride solution wash-out, eluent flow rate is 30mL/h, fraction collection, every pipe 3mL, sulfuric acid-phynol method is followed the tracks of and is detected (490nm), and record data are also drawn elution curve.Collect and merge each peak pipe, low temperature deionized water dialysis 48 hours, freeze-drying.Carry out the scanning of ultraviolet all wave band (190-400nm) and KBr compressing tablet infrared scan (500-4000cm
-1).
Claims (4)
1. the purification process of a green tea polysaccharide, it is characterized in that: the tea polysaccharide crude extract is through DEAE C-52 cellulose column and Sephadex G-100 gel filtration chromatography, obtain the tea polysaccharide behind the purifying, the scanning of ultraviolet all wave band does not contain other components such as nucleic acid or protein, infared spectrum shows, at 500-4000cm
-1The district has the general feature absorption peak of polysaccharose substance.
2. the purification process of a kind of green tea polysaccharide according to claim 1, it is characterized in that: DEAE C-52 cellulose column on the thick tea polysaccharide, behind the sodium-chlor gradient elution with 0.1-0.5mol/L, the 10th pipe goes out the peak, the elutriant that collection obtains, last Sephadex G-100 gel column, the 28th pipe goes out the peak, and peak shape is single symmetry.
3. the purification process of a kind of green tea polysaccharide according to claim 2, the tea polysaccharide ultraviolet all wave band that is obtained scanning shows: a tangible absorption peak is arranged at the 200nm place, and at 260nm, 280nm etc. locate all not have absorption.
4. the purification process of a kind of green tea polysaccharide according to claim 2, the tea polysaccharide infrared spectrogram that is obtained shows: at 500-4000cm
-1The district all has the general feature absorption peak of polysaccharose substance, as 1724cm
-1The methoxy ester group at place or O-ethanoyl-stretching vibration of C=O, 1144-1020cm
-1Between ehter bond (C-O-C) stretching vibration of pyranose ring and the absorption peak of hydroxyl, 954cm
-1The roll vibration of the methyne of the desoxy sugar at place, 893cm
-1The C-H angle vibration of β type anomerism, 856cm
-1The characteristic absorbance of the furanose at place, 835cm
-1The C-H angle vibration of α type anomerism.
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CN 201110045110 CN102153667A (en) | 2011-02-25 | 2011-02-25 | Method for purifying green tea polysaccharides |
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CN 201110045110 CN102153667A (en) | 2011-02-25 | 2011-02-25 | Method for purifying green tea polysaccharides |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109081879A (en) * | 2018-08-09 | 2018-12-25 | 铜仁学院 | A kind of technique for extracting Mount Fanjing black tea polysaccharide |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1958617A (en) * | 2006-11-28 | 2007-05-09 | 湖北丽益医药科技有限公司 | Tea polysaccharide, preparation method, and usage |
CN101497906A (en) * | 2008-07-01 | 2009-08-05 | 南昌大学 | Separation and purification preparation as well as hypoglycemic activity of tea polysaccharide |
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2011
- 2011-02-25 CN CN 201110045110 patent/CN102153667A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1958617A (en) * | 2006-11-28 | 2007-05-09 | 湖北丽益医药科技有限公司 | Tea polysaccharide, preparation method, and usage |
CN101497906A (en) * | 2008-07-01 | 2009-08-05 | 南昌大学 | Separation and purification preparation as well as hypoglycemic activity of tea polysaccharide |
Non-Patent Citations (2)
Title |
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《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 20090315 吴晓鹏 苦丁茶(Ilex kudingcha C.J.Tseng)多糖提取、分离、纯化及部分生物活性研究 第18-24页 1-4 , * |
《茶叶科学》 20081231 寇小红等 膜过滤绿茶多糖的系统分级纯化及免疫活性分析 第172-180页 1-4 第28卷, 第3期 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109081879A (en) * | 2018-08-09 | 2018-12-25 | 铜仁学院 | A kind of technique for extracting Mount Fanjing black tea polysaccharide |
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Application publication date: 20110817 |