CN102150617A - Automatic plant tissue culturing machine - Google Patents
Automatic plant tissue culturing machine Download PDFInfo
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- CN102150617A CN102150617A CN 201110001336 CN201110001336A CN102150617A CN 102150617 A CN102150617 A CN 102150617A CN 201110001336 CN201110001336 CN 201110001336 CN 201110001336 A CN201110001336 A CN 201110001336A CN 102150617 A CN102150617 A CN 102150617A
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- microprocessor
- light source
- storage tank
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses an automatic plant tissue culturing machine, relating to a plant tissue culturing device. The device comprises a motor remote control circuit, a motor, a light source, a water storage tank, a microprocessor, a display circuit, a residual liquid groove, a carbon dioxide storage tank, a residual liquid collection mechanism, a temperature-humidity regulator, sensors, a fan, a liquid spraying turntable, a filter screen and a spongy layer; wherein the motor remote control circuit, the motor, the light source and the water storage tank are arranged in a housing; the light source and the water storage tank are arranged below the motor; the liquid spraying turntable is arranged below the light source and the water storage tank and fixedly connected to a shaft of the motor; the three sensors are arranged at the upper part in the housing and connected with the microprocessor; the motor remote control circuit is electrically connected with the microprocessor; the microprocessor, the display circuit and the fan are arranged on the side face of the housing, the display circuit is connected with the microprocessor; the residual liquid groove is arranged at the bottom of the housing; the residual liquid collection mechanism is arranged above the residual liquid groove; the filter screen is arranged above the residual liquid collection mechanism; the spongy layer is arranged on the filter screen; and the carbon dioxide storage tank and the temperature-humidity regulator are arranged on the outer wall of the housing.
Description
Technical field
The present invention relates to a kind of plant culture equipment, especially relate to a kind of automation Plant Tissue Breeding machine.
Background technology
Tissue culture is to use the sterile working method to cultivate biological isolated organ, tissue or cell, makes the technology of its g and D under artificial condition, also is the technology of cultivating under external artificial condition from the isolated tissue of body or cell.
Plant Tissue Breeding refers to isolate tissue, organ or cell, the protoplast etc. that suit the requirements from plant corpus, by sterile working, under the manual control condition, cultivate with whole plant that obtains regeneration or the technology of producing other products of economically valuable.That is to say, the tissue culture technique of plant has developed 171 years, though the principle of Plant Tissue Breeding is comparatively ripe, but the tissue culture plant is still adopted Artificial Control condition of culture, culture environment, also be in a job that consumes a large amount of man power and materials, relevant device relatively lags behind, and also rarely has report.
Chinese patent CN2399926 discloses a kind of nothing soil cultivating machine that is exclusively used in the assembled boats and ships of boats and ships with nutritive vegetable, be made up of air conditioner, computer, culturing room etc., adopt the housing assembled, the door of raising chamber is a glass door, be convenient to observation, light harvesting, mechanical, electrical, air-conditioning is an one.
Chinese patent CN101715722A discloses a kind of full-automatic no-soil plant culture apparatus, comprises base and multiple culture dish that nutrient solution deposit bucket and its underpart are provided with, also comprises at least one condensed water maker.Described condensed water maker is made up of the coolant pump on metal heat-conducting silk, organic heat conducting film, temperature control water level switch, semiconductor refrigerating heap and semiconductor refrigerating heap top.This is invented independent culture dish and can peg graft, and flexibility is bigger, forms a plurality of culture dishes, cultivates a plurality of plants simultaneously, and according to the difference of plant water requirement, controls the water level of every plant targetedly.
Summary of the invention
The object of the present invention is to provide a kind of automation Plant Tissue Breeding machine.
The present invention is provided with casing, hood, motor remote control circuit, motor, light source, aqua storage tank, microprocessor, humidity sensor, display circuit, drain tank, carbon dioxide storage jar, surplus liquid collecting mechanism, optical sensor, temperature humidity adjuster, temperature sensor, blower fan, spray liquid rotating disk, filter screen and spongy layer;
Hood is located at the casing top, motor remote control circuit, motor, light source and aqua storage tank are located in the hood, motor is electrically connected with motor remote control circuit, and light source and aqua storage tank are located at the motor below, and the spray liquid rotating disk is located at light source and aqua storage tank below and is fixed on the arbor of motor; Humidity sensor, optical sensor and temperature sensor are located at the casing internal upper part, and the output signal end of the output signal end of humidity sensor, the output signal end of optical sensor and temperature sensor all is connected with the input of microprocessor; Motor remote control circuit is electrically connected with the output of microprocessor, and microprocessor, display circuit and blower fan are located on the casing side, the output of the input termination microprocessor of display circuit; Drain tank is located at bottom of shell, and surplus liquid collecting mechanism is used as a servant in the drain tank top, and filter screen is located at surplus liquid collecting mechanism top, and spongy layer is located on the filter screen; Carbon dioxide storage jar and temperature humidity adjuster are located on the casing outer wall.
Can be provided with acid-base neutralization powder box, water injection hole and weeper on the described hood.
Described light source can adopt LED light lamp.
The present invention adopts the means of automation, and tissue culture is carried out at the stripped plant of plant, body or other position.People only need pass through computer, the every demand when just can controlling plant cultivating.
Compare with existing plant culture equipment, the present invention has following outstanding advantage:
1) owing to is provided with system and the 3 kinds of sensors such as temperature, humidity and illumination that are used for moisture, carbon dioxide, radiation adjusting, therefore can utilize the most basic three elements in the plant cultivation, implement the real-time numerical information of the plant of monitoring feedback automatically culture environment, can spray, discharge the culture fluid of needs and carbon dioxide etc. in real time according to the technical parameter of setting, the confession plant growing is required.
2) in general, the cultivation of plant need be made a medium or desinfection chamber in advance by the researcher, and the present invention's employing is that the spongy layer retaining is cultivated, at cultivation region spongy layer is set, and store the situations such as content of culture fluid by the sensor senses spongy layer, to plant drip irrigation and spray irrigation culture fluid, guarantee the growth demand of plant by system-computed and the aqua storage tank that is arranged on the hood top in good time, also can carry out by remote control.
3) light source of the present invention can adopt LED (light emitting diode) lamp, by setting the formation of lamp, by optical sensor perception intensity of illumination, and controls intensity of illumination automatically by computer.
4) owing to be provided with motor remote control circuit and microprocessor etc., therefore can control, simulate the various places real time environment as required and cultivate, adopt automation to cultivate means, the Plant Tissue Breeding flow process that control is complicated by computer programming.Simultaneously the plant cultivation region can be in time changed as required,, multiple plant type plant growing can be fit to adapt to the cultivation of medium and small plant and stripped callus.
5), therefore be convenient to shift carry, so that be adapted to various needs because volume of the present invention is less.
6) the present invention is particularly useful for student, scientific and technological fan, plant and cultivates the fan and carry out activities such as Research Learning, scientific research, make colonies such as student convenient, carry out Plant Tissue Breeding and scientific research observation activity efficiently, cultivate student's quality of science, mobilizing students scientific research enthusiasm.
Description of drawings
Fig. 1 is that the structure of the embodiment of the invention is formed schematic diagram.
Fig. 2 is that the left TV structure of Fig. 1 is formed schematic diagram.
Fig. 3 is that the plan structure of Fig. 1 is formed schematic diagram.
Embodiment
Following examples will the present invention is further illustrated in conjunction with the accompanying drawings.
Referring to Fig. 1～3, the embodiment of the invention is provided with casing A, hood B, motor remote control circuit 1, motor 2, light source 3, aqua storage tank 4, microprocessor 5, humidity sensor 6, display circuit 7, drain tank 8, carbon dioxide storage jar 9, surplus liquid collecting mechanism 10, optical sensor 11, temperature humidity adjuster 12, temperature sensor 13, blower fan 14, spray liquid rotating disk 15, acid-base neutralization powder box 16, filter screen 17 and spongy layer 18.
Hood B is located at casing A top, motor remote control circuit 1, motor 2, light source 3 and aqua storage tank 4 are located in the hood B, motor 2 is electrically connected with motor remote control circuit 1, light source 3 and aqua storage tank 4 are located at motor 2 belows, and spray liquid rotating disk 15 is located at light source 3 and aqua storage tank 4 belows and is fixed on the arbor of motor 2; Humidity sensor 6, optical sensor 11 and temperature sensor 13 are located at casing A internal upper part, and the output signal end of the output signal end of humidity sensor 6, optical sensor 11 all is connected with the input of microprocessor 5 with the output signal end of temperature sensor 13; Motor remote control circuit 1 is electrically connected with the output of microprocessor 5, and microprocessor 5, display circuit 7 and blower fan 14 are located on the casing A side, the output of the input termination microprocessor 5 of display circuit 7; Drain tank 8 is located at casing A inner bottom part, and surplus liquid collecting mechanism 10 is used as a servant in drain tank 8 tops, and filter screen 17 is located at surplus liquid collecting mechanism 10 tops, and spongy layer 18 is located on the filter screen 17; Carbon dioxide storage jar 9 and temperature humidity adjuster 12 are located on the casing A outer wall.
Also be provided with water injection hole 19 and weeper 20 on the described hood B.Described light source adopts the LED light lamp array.
Below provide and adopt the present invention to cultivate the example of plant callus callus of induce tissue culture, observed data is as shown in table 1.
Table 1
| The 1st week | The 2nd week | The 3rd week | The 4th week | |
| Callus cell group's diameter | 2.44mm | 9.07mm | 12.11mm | 12.77mm |
| Whether the bud clump appears | Not | Not | Not | Chlorenchyma occurs |
| Whether disease sign/character variation appears | Do not have | Do not have | Do not have | Do not have |
Sponge cultivation region culture fluid is MS+ sucrose 3%+ agar 0.7%+6-BA2.0mg/L+NAA2.0mg/L
The observed data of being cultivated plant callus callus of induce tissue culture by the normal tissue cultural method is as shown in table 2.
Table 2
| The 1st week | The 2nd week | The 3rd week | The 4th week | |
| Callus cell group's diameter | 2.44mm | 5.12mm | 9.71mm | 11.68mm |
| Whether the bud clump appears | Not | Not | Not | Chlorenchyma occurs |
| Whether disease sign/character variation appears | Do not have | Do not have | Do not have | Do not have |
Sponge cultivation region culture fluid is MS+ sucrose 3%+ agar 0.7%+6-BA2.0mg/L+NAA2.0mg/L.
Claims (3)
1. automation Plant Tissue Breeding machine is characterized in that being provided with casing, hood, motor remote control circuit, motor, light source, aqua storage tank, microprocessor, humidity sensor, display circuit, drain tank, carbon dioxide storage jar, surplus liquid collecting mechanism, optical sensor, temperature humidity adjuster, temperature sensor, blower fan, spray liquid rotating disk, filter screen and spongy layer;
Hood is located at the casing top, motor remote control circuit, motor, light source and aqua storage tank are located in the hood, motor is electrically connected with motor remote control circuit, and light source and aqua storage tank are located at the motor below, and the spray liquid rotating disk is located at light source and aqua storage tank below and is fixed on the arbor of motor; Humidity sensor, optical sensor and temperature sensor are located at the casing internal upper part, and the output signal end of the output signal end of humidity sensor, the output signal end of optical sensor and temperature sensor all is connected with the input of microprocessor; Motor remote control circuit is electrically connected with the output of microprocessor, and microprocessor, display circuit and blower fan are located on the casing side, the output of the input termination microprocessor of display circuit; Drain tank is located at bottom of shell, and surplus liquid collecting mechanism is used as a servant in the drain tank top, and filter screen is located at surplus liquid collecting mechanism top, and spongy layer is located on the filter screen; Carbon dioxide storage jar and temperature humidity adjuster are located on the casing outer wall.
2. automation Plant Tissue Breeding machine as claimed in claim 1 is characterized in that described hood is provided with acid-base neutralization powder box, water injection hole and weeper.
3. automation Plant Tissue Breeding machine as claimed in claim 1 is characterized in that described light source is a LED light lamp.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110001336A CN102150617B (en) | 2011-01-05 | 2011-01-05 | Automatic plant tissue culturing machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110001336A CN102150617B (en) | 2011-01-05 | 2011-01-05 | Automatic plant tissue culturing machine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102150617A true CN102150617A (en) | 2011-08-17 |
| CN102150617B CN102150617B (en) | 2012-09-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110001336A Expired - Fee Related CN102150617B (en) | 2011-01-05 | 2011-01-05 | Automatic plant tissue culturing machine |
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| Country | Link |
|---|---|
| CN (1) | CN102150617B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1142534A (en) * | 1996-05-15 | 1997-02-12 | 中国科学院化工冶金研究所 | Reactor for nutrient liquid atomizing culture of plant |
| CN201919455U (en) * | 2011-01-05 | 2011-08-10 | 苏晖阳 | Plant tissue culture device |
-
2011
- 2011-01-05 CN CN201110001336A patent/CN102150617B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1142534A (en) * | 1996-05-15 | 1997-02-12 | 中国科学院化工冶金研究所 | Reactor for nutrient liquid atomizing culture of plant |
| CN201919455U (en) * | 2011-01-05 | 2011-08-10 | 苏晖阳 | Plant tissue culture device |
Non-Patent Citations (1)
| Title |
|---|
| 《中国农业科学》 19871231 姜长阳等 植物组织培养中一种新的支持物 96 1-3 第20卷, 第1期 * |
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| Publication number | Publication date |
|---|---|
| CN102150617B (en) | 2012-09-05 |
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