CN102115714A - Method for performing open cultivation of diatom by adding silicon - Google Patents
Method for performing open cultivation of diatom by adding silicon Download PDFInfo
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- CN102115714A CN102115714A CN 201010587734 CN201010587734A CN102115714A CN 102115714 A CN102115714 A CN 102115714A CN 201010587734 CN201010587734 CN 201010587734 CN 201010587734 A CN201010587734 A CN 201010587734A CN 102115714 A CN102115714 A CN 102115714A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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Abstract
The invention provides a method for performing open cultivation of diatom by adding silicon. The method comprises the following steps of: inoculating diatom-containing diatom seed liquid into an open microalgae culture apparatus; and adding carbon dioxide and a nutritive salt to form culture fluid for performing diatom cultivation. The method is characterized in that: sufficient silicon elements are added under control during adding of the nutritive salt, and the silicon elements are added in an amount that the silicon elements reach saturation solubility in the culture fluid. The method has the advantages of realization of industrial large-scale open cultivation of diatom, simple process condition, easiness in controlling and low cultivation cost.
Description
Technical field
The present invention relates to the both culturing microalgae technology, relate to the cultural technique of diatom particularly.
Background technology
The training method of existing little algae has closed and open two kinds.Open being meant adopted open pond culture apparatus, as runway pond, circular shallow pond, it possesses skills simply, invests characteristics such as cheap, exists various little algae algae kinds owing to cultivate in the water body in open little algae, and the growth of vying each other of various little algae algae kind, the influence that ingested by assorted algae pollution and zooplankton, the target algae can not account for advantage, in breeding environment a little less than the competitive power, causes in the aquaculture water target algae cell density low, kind is changeable, can't form suitability for industrialized production.Be subjected to the restriction of above-mentioned technological deficiency, in the at present open both culturing microalgae technology, have only spirulina, chlorella and salt algae etc. a few has obtained application in suitability for industrialized production, and is difficult to turn out single diatom.
Existing diatom is cultured and can adopt the closed culture apparatus to culture, closed is meant the bioreactor of the closed that adopts different structure, as air lift type, stirring-type, tubular type etc., carry out the diatom breed by the nutrient solution that in bioreactor, adds the suitable growth of diatom algae for preparing in advance.Closed is cultivated and is required envrionment conditions stable, and therefore difficult in maintenance, cost is too high during large scale culturing.
The a large amount of C of the growth needs of diatom, N, P and Si element, the suitable concn of each nutritive salt can promote the high-density growth of diatom in the assurance nutrient solution, as patent CN200310120738.3 disclosed " cultural method of diatomaceous liquid, diatom and diatom " is exactly in the closed photo sundries, carries out the diatom breed by making the nutrient concentration proportioning that is fit to growth of diatom algae.The seeding tank that closed both culturing microalgae is suitable for production high value products (as medicine or healthcare products) or cultivates as open pond, its technology controlling and process complexity, need constantly the various nutrient composition contents in the nutrient solution to be measured, and then replenish various nutritive ingredients quantitatively to keep predetermined cultivation concentration, for diatom, the shortcoming of closed culture is that to cultivate cost too high and can't industrialization.
Summary of the invention
The present invention provides a kind of the utilization to add the method that silicon carries out the open breed of diatom for solving the open culture technique difficult problem of diatom.
The present invention realizes that the technical scheme that goal of the invention adopts is, a kind of utilization added the method that silicon carries out the open breed of diatom, adopt open micro algae culturing device, the algae seedling solution that at first will contain diatom inserts described open micro algae culturing device, add carbonic acid gas and nutritive salt then and carry out the diatom breed to form nutrient solution, it is characterized in that: control adds the element silicon of capacity when adding nutritive salt, and add-on is to reach the saturation solubility of element silicon in nutrient solution.
Better, after adding the element silicon and the solubleness that reaches capacity, continue to keep the saturation solubility of element silicon in nutrient solution.
Since diatom can be constantly in process of growth the consume silicon element, better, adopt a small amount of method repeatedly to add element silicon and continue to keep the saturation solubility of element silicon in nutrient solution.
Better, as a kind of method simply and easily, standard in nutrient solution, occurs being precipitated as with element silicon and judge the reach capacity solubleness of element silicon in nutrient solution.
The invention has the beneficial effects as follows:
When 1, adding element silicon and other nutritive salt at the cultivation initial stage, capacity is added in utilization in nutrient solution element silicon makes the element silicon solubleness that reaches capacity, because growth of diatom algae speed is fast in this nutrient solution environment, has tangible competitive edge, can in competition, win the growth of other algae kinds outside the target of breeding in a large number, and then the inhibition in a short time algae diatom, highdensity diatom nutrient solution is stablized in formation, thereby the large-scale industrialization that has realized the open breed of diatom is cultivated;
2, another unusual effect of the present invention is, processing condition are simple, is easy to control, and aquaculture cost is low.
Embodiment
A kind of utilization added the method that silicon carries out the open breed of diatom, adopts open micro algae culturing device, all can as runway pond, circular shallow pond etc.The algae seedling solution that at first will contain diatom inserts in the reactive agent of open micro algae culturing device, algae seedling solution can be selected fresh seawater or natural fresh water, fresh seawater or natural fresh water all contain diatom and other various little algae algae kinds, add carbonic acid gas as carbon source, add nitrogen (N), phosphorus (P) and silicon (Si) element carry out the diatom breed as nutritive salt to form nutrient solution, and control adds the element silicon of capacity, add-on is to reach the saturation solubility of element silicon in nutrient solution, growth along with diatom, element silicon is constantly consumed, therefore can adopt a small amount of method repeatedly to continue to add element silicon, to keep the saturation solubility of element silicon in nutrient solution, avoid disposable excessive adding element silicon to produce waste simultaneously, during concrete enforcement, the judging criterion of small amount of precipitate as the solubleness that whether reaches capacity arranged in nutrient solution to guarantee element silicon.
It should be noted that at last: above embodiment only in order to the explanation the present invention and and unrestricted technical scheme described in the invention; Therefore, it will be understood by those of skill in the art that still and can make amendment or be equal to replacement the present invention although this specification sheets has been described in detail the present invention with reference to each above-mentioned embodiment; And all do not break away from the technical scheme and the improvement thereof of the spirit and scope of the present invention, and it all should be encompassed in the claim scope of the present invention.
Claims (4)
1. one kind is utilized and adds the method that silicon carries out the open breed of diatom, adopt open micro algae culturing device, the algae seedling solution that at first will contain diatom inserts described open micro algae culturing device, add carbonic acid gas and nutritive salt then and carry out the diatom breed to form nutrient solution, it is characterized in that: control adds the element silicon of capacity when adding nutritive salt, and add-on is to reach the saturation solubility of element silicon in nutrient solution.
2. a kind of utilization the according to claim 1 added the method that silicon carries out the open breed of diatom, it is characterized in that: continue to keep the saturation solubility of element silicon in nutrient solution after adding the element silicon and the solubleness that reaches capacity.
3. a kind of utilization the according to claim 1 and 2 added the method that silicon carries out the open breed of diatom, it is characterized in that: adopt a small amount of method repeatedly to add element silicon and continue to keep the saturation solubility of element silicon in nutrient solution.
4. a kind of utilization the according to claim 3 added the method that silicon carries out the open breed of diatom, it is characterized in that: standard occurs being precipitated as with element silicon in nutrient solution and judge the reach capacity solubleness of element silicon in nutrient solution.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010587734 CN102115714A (en) | 2010-12-07 | 2010-12-07 | Method for performing open cultivation of diatom by adding silicon |
| PCT/CN2011/077118 WO2012075812A1 (en) | 2010-12-07 | 2011-07-13 | Method for openly cultivating diatom by adding silicon |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010587734 CN102115714A (en) | 2010-12-07 | 2010-12-07 | Method for performing open cultivation of diatom by adding silicon |
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| Publication Number | Publication Date |
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| CN102115714A true CN102115714A (en) | 2011-07-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201010587734 Pending CN102115714A (en) | 2010-12-07 | 2010-12-07 | Method for performing open cultivation of diatom by adding silicon |
Country Status (2)
| Country | Link |
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| CN (1) | CN102115714A (en) |
| WO (1) | WO2012075812A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012075812A1 (en) * | 2010-12-07 | 2012-06-14 | 深圳市兆凯生物工程研发中心有限公司 | Method for openly cultivating diatom by adding silicon |
| CN104030451A (en) * | 2014-06-19 | 2014-09-10 | 张家港保税区青山绿水生物科技有限公司 | Method for restoring water body by directionally breeding aboriginal diatom |
| CN115926991A (en) * | 2022-12-02 | 2023-04-07 | 中国科学院广州地球化学研究所 | A method for reducing the solubility of diatom shells and low-solubility diatoms and applications |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4333263A (en) * | 1980-10-07 | 1982-06-08 | The Smithsonian Institution | Algal turf scrubber |
| CN1504109A (en) * | 2002-11-28 | 2004-06-16 | 雅马哈发动机株式会社 | Liquid containing diatom, diatom and method for culturing diatom |
| CN101519634A (en) * | 2009-03-27 | 2009-09-02 | 兆凯生物工程研发中心（深圳）有限公司 | Open type biological reactor for microalgae cultivation and microalgae cultivation method |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100400642C (en) * | 2004-06-18 | 2008-07-09 | 徐州师范大学 | An open culture method of Phaeodactylum tricornutum and its special medium |
| CN102115714A (en) * | 2010-12-07 | 2011-07-06 | 王兆凯 | Method for performing open cultivation of diatom by adding silicon |
-
2010
- 2010-12-07 CN CN 201010587734 patent/CN102115714A/en active Pending
-
2011
- 2011-07-13 WO PCT/CN2011/077118 patent/WO2012075812A1/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4333263A (en) * | 1980-10-07 | 1982-06-08 | The Smithsonian Institution | Algal turf scrubber |
| CN1504109A (en) * | 2002-11-28 | 2004-06-16 | 雅马哈发动机株式会社 | Liquid containing diatom, diatom and method for culturing diatom |
| CN101519634A (en) * | 2009-03-27 | 2009-09-02 | 兆凯生物工程研发中心（深圳）有限公司 | Open type biological reactor for microalgae cultivation and microalgae cultivation method |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012075812A1 (en) * | 2010-12-07 | 2012-06-14 | 深圳市兆凯生物工程研发中心有限公司 | Method for openly cultivating diatom by adding silicon |
| CN104030451A (en) * | 2014-06-19 | 2014-09-10 | 张家港保税区青山绿水生物科技有限公司 | Method for restoring water body by directionally breeding aboriginal diatom |
| CN104030451B (en) * | 2014-06-19 | 2016-05-11 | 张家港保税区青山绿水生物科技有限公司 | A kind of method that directive breeding original inhabitants diatom is repaired water body |
| CN115926991A (en) * | 2022-12-02 | 2023-04-07 | 中国科学院广州地球化学研究所 | A method for reducing the solubility of diatom shells and low-solubility diatoms and applications |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012075812A1 (en) | 2012-06-14 |
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Application publication date: 20110706 |