CN102099691A - Method of using a flow cell apparatus for visualizing additive deposition on a substrate - Google Patents
Method of using a flow cell apparatus for visualizing additive deposition on a substrate Download PDFInfo
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- CN102099691A CN102099691A CN2009801277875A CN200980127787A CN102099691A CN 102099691 A CN102099691 A CN 102099691A CN 2009801277875 A CN2009801277875 A CN 2009801277875A CN 200980127787 A CN200980127787 A CN 200980127787A CN 102099691 A CN102099691 A CN 102099691A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N2021/0346—Capillary cells; Microcells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/05—Flow-through cuvettes
Abstract
Disclosed is a method of visualizing the deposition of an additive from a personal care composition onto substrates such as hair, skin mimic and fabric through the use of a flow cell device.
Description
Invention field
The adjuvant deposition visualization method that the present invention relates to utilize flow cell equipment to make and deposit to the adjuvant on the stromal surface, described matrix is for example hair, bionics skin or fabric.
Background of invention
With regard to most industry, the particle deposition on measurement and the visual matrix is extremely important.Medical skill has developed into foreign particle deposition of estimating on the lung tissue and the danger of estimating blood vessel blockage.For measurement of electrostatic attracts, developed the method for the rate of sedimentation that is used to measure the electrically charged particle on the matrix.Utilized computer simulation in addition so that observe particle deposition as the means of measuring air filter efficiency.These technology generally provide a description its result's quantitative data.Explain that these data need the professional training of association area or certain auxiliary process with reception and registration trend and effect usually.Therefore, these technology can not be provided for passing on based on the beneficial effect of the particle deposition on the matrix or the effective means of infringement.In addition, there is not one the environment that wherein be can be observed particle deposition time in the multiple additives importing system is provided in these technology.
The flow cell unit is people known (referring to US 4,974,952) and various medicines and adjuvant is imported in the fluid environment to analyze its effect through being usually used in.In general, the flow cell unit application is in large number of biological research is used.Flow cell is generally used in the flow cytometer structure.This type of device can be used for analyzing p.s. thousands of particles, and can initiatively separate and isolate the particle with specified performance.Flow cytometry is associated with using such as the cell biological of fluorescence-activated cell sorting the most commonly.Yet this technology also has been widely used in the experiment of medical treatment and bioengineering.
Attempted utilizing flow cell to make the particle deposition on the matrix visual.This technology is applied to the visual previous trial of granulate-matrix, and to have utilized the flow cell differential interference phase-contrast microscope to make with the interactional coacervate of hair fiber visual.J.Cosmet.Sci.，58，637-650(11/12，2007)。Coacervate importantly helps to nurse one's health particle deposition to hair fiber mutually, and the flow cell differential interference phase-contrast microscope is used to make the deposition efficiency of the multiple personal care composition on the hair matrix visual.
Yet previous flow cell is specified the position of the hair fiber between two slide glasses, makes liquid solution pass through it and flows.This configuration causes inefficient system, spreads all over whole hair fibers because liquid solution can not flow, and this is because between two flow cell slide glasses that fiber " is clipped in " and both all come in contact.Therefore, liquid solution can only interact with the substrate edge that does not contact slide glass.Therefore, during analyzing, only the edge of visible matrix is exposed to liquid solution and visual variation.In addition, " sandwich " often causes adjuvant to deposit on the surface of slide glass, and this is because there are not enough spaces to make some particles by this system.Therefore, the deposition on the matrix is difficult to disturb adjuvant to distinguish with other.
Described based on preamble, need be provided for the flow cell equipment of the device of the interactional visual analysis of granulate-matrix, wherein expose bigger substrate surface area and be used for analyzing.
Summary of the invention
The present invention relates to be used to make adjuvant deposition visualization method, said method comprising the steps of: (a) preparation comprises the thinning agent of personal care composition and water, and described personal care composition comprises adjuvant; (b) matrix is placed the flow cell that comprises flow cell compartment and flow channel, described flow cell compartment comprises fluid volume capacity and four or more a plurality of flow cell locular wall; Described matrix is suspended in the flow cell compartment, makes matrix not contact plural flow cell locular wall and matrix is positioned at flow channel; (c) thinning agent is expelled in the flow cell; (d) visual signal of record flow cell compartment.
The accompanying drawing summary
Accompanying drawing shows embodiment of the present invention, and with general remark of the present invention that above provides and the detailed Description Of The Invention that hereinafter provides, is used for explaining the present invention.
Fig. 1 is the exploded view of flow cell equipment;
Fig. 2 is the sectional view according to little ditch slotted vane of the arrow 2-2 of Fig. 1;
Fig. 3 A is at the image of taking during the flow cell unit operations of this paper in the presence of the shampoo Compositions;
The image of Fig. 3 B for during flow cell unit operations after the shampoo Compositions of flow cell unit upper punch map brushing 3A, taking at this paper;
The image of Fig. 4 A for during the flow cell unit operations at this paper in the presence of the shampoo Compositions that comprises particle and deposition aid, taking;
The image of Fig. 4 B for during flow cell unit operations after the shampoo Compositions that comprises particle and deposition aid of flow cell unit upper punch map brushing 4A, taking at this paper.
Detailed Description Of The Invention
The present invention relates to be used to make the particle deposition visualization method, said method comprising the steps of: preparation comprises the thinning agent of adjuvant, matrix is placed the flow cell that comprises flow cell compartment and flow channel, be expelled to thinning agent in the flow cell and write down the visual pattern of flow cell compartment.
The careful fluid volume capacity of controlling the also careful selection flow cell compartment of diluent mass flow ratio that flow in the flow cell compartment has produced the environment of the adjuvant visual pattern that deposits on the matrix.
Thinning agent
Personal care composition often is sold to the consumer with the form of further being diluted by the consumer during using.For example, shampoo and conditioner composition are often assigned to being administered on the hair on hand and subsequently of user.Therefore between the operating period, often water is added on the hair, mix with shampoo and conditioner composition and dilute, this often is called " in the use " situation.Therefore, the actual customer situation in the time of should selecting dilution ratio to utilize personal care composition to be formed for thinning agent in this paper method with reflection.
The dilution ratio of personal care composition will depend on the viscosity and the rheology of the personal care composition that relates to, and for example can not stop thinning agent to pass through the transmission of flow cell passage but should fully dilute.
In addition, must select dilution ratio to make thinning agent " as seen " (hereinafter discuss) in flow cell compartment." as seen " example is found among Fig. 4 A." as seen " used herein be meant adjuvant in thinning agent and the thinning agent with multiplying arrangement as seen and not necessarily by naked eyes.
The dilution ratio of fluid composition and water should be about 1: 1 to about 1: 150, and more preferably 1: 2 to about 1: 50, and most preferably from about 1: 2 to about 1: 9.In one embodiment, with the ratio mixing shampoo Compositions of water described composition is formed thinning agent by composition with about 1: 2 to about 1: 10 (for example, 1: 9).In one embodiment, with the ratio mixing conditioner composition of water described composition is formed thinning agent by composition with about 1: 70 to about 1: 150 (for example, 1: 100).In one embodiment, with the ratio mixing skin care compositions (for example bath agent) of water described composition is formed thinning agent by composition with about 1: 1 to about 1: 10 (for example, 1: 2 or 1: 9).In one embodiment, by with about 1: 1 to the ratio mixed goods care composition (for example fabric softener) of about 1: 100 composition and water and described composition is formed thinning agent.
Adjuvant
As used herein, term " adjuvant " is meant and is present in any composition that makes in its fluid that flows through the flow cell passage and visually be different from surrounding fluid when observing by microscope or other multiplying arrangements.These adjuvants are characterized by the material that utilizes personal care composition to obtain specific beneficial effect (for example, conditioning property, flexibility, moisture retention, plentiful property) best.
For example, in such as the hair care composition of shampoo Compositions, such as the skin care compositions of bath agent composition and in the Fabrid care composition, often utilize coacervate to be provided for the carrier of active substance, described active substance provides conditioning property, moisture retention or flexibility beneficial effect.Coacervate in the shampoo Compositions formed in when dilution, and the association of generation is separated when interacting with the critical association constant for anionic surfactant and cationic polymer.Other information is referring to Polymer-SurfactantInteraction, and Jan C.T.Kwak edits, surfactant science series the 77th volume, Marcel Dekker, Inc.New York (1998).The coacervate that contains such as the adjuvant of siloxane deposits on the hair surface subsequently.Equally, in one embodiment, adjuvant can be counted as containing or not containing the coacervate of silicone compositions.
The skin care compositions utilization contains the coacervate of vaseline material.Fabrid care composition also utilizes silicone compositions.
In conditioner, the fatty alcohol and the surfactant gel network of composition utilization and silicone compositions combination.Referring to US 2006/0078528A1.
The suitable material that is used for adjuvant comprises those that following patent is discussed: US2007/0207109A1, US 2005/01582661A1, US 2006/0024381A1, US2007/0010408A1, US7528099B2.
Yet adjuvant also can be by depositing on the surface such as the mechanism of filtering, and wherein thinning agent is in the mechanical motion on the stromal surface or flow and make it contact and remain on the stromal surface with stromal surface.
Matrix
The suitable matrix that is used for this paper method comprises sends out tuft, bionics skin and fabric sample.Matrix is positioned in the flow cell compartment to contact with flow channel.Depend on matrix, the orientation (hereinafter discuss) of matrix in flow cell compartment will influence adjuvant and how to deposit on the stromal surface.In one embodiment, single hair or many hairs are as matrix.Described single or many hairs extend to the other end by an end of flow cell compartment at axial orientation on the flow channel direction at thinning agent, thereby see the deposition effect of thinning agent on hair surface.In another embodiment, many hairs are axially perpendicular to the flow channel of thinning agent to see the filter effect of thinning agent on hair surface.
Send out tuft or a plurality of tufts and can be used as matrix.Depend on desired visual type, can utilize virgin hair sample (hair of Gai Bianing non-chemically) and damaged hair sample (that is, adjuvant deposit to original and/or damaged hair on).The commercially available acquisition of wig for example derives from Hugo Royer Intemational Limited (10 Lakeside Business Park, Sandhurst, Berkshire, GU47 9DN, England) those.
Bionics skin can be used as matrix, especially when the skin care compositions that utilizes such as bath agent.Other information about bionics skin can be present among the WO08/084442A1.
Fabric: can utilize the natural/synthon of natural fiber, synthon or mixing, for example silk, wool, cotton, rayon, nylon, polyester, lycra and spandex.
Flow cell
Flow cell is known as device and the commercially available acquisition of describing among the US 4,974,952 for example, for example derives from the Focht flow cell of Bioptechs.Suitable flow cell (10) is shown as exploded view in Fig. 1.Flow cell (10) comprises chamber frame (12), upper gasket (14), little ditch slotted vane (16), first pad (18), matrix (20), second pad (22), microslide (24), lower gasket (26) and back up pad (28).Must select flow cell (10) to make it matrix can be remained in the flow cell compartment (32), flow channel (30) is provided, and flow cell arrangements must be become make light can pass through flow cell (10) and flow cell compartment (32).Equally, select chamber frame (12), upper gasket (14), little ditch slotted vane (16), first pad (18), second pad (22), microslide (24), lower gasket (26) and back up pad (28), make at least a portion of these assemblies comprise center pit or comprise the clear area.In addition, the center pit of these assemblies or clear area make light can pass through flow cell (10) along the similar Z-axis alignment by flow cell (10).In one embodiment, first packing ring (18) and second packing ring (22) comprise center pit, make packing ring of winning (18) and second packing ring (22) have internal edge and external margin, and internal edge are concentric with external margin.
When assembling, flow cell (10) demonstrates flow channel (30) to be used for that thinning agent is expelled to flow cell compartment (32), passes flow cell compartment (32) and leaves flow cell compartment (32) subsequently.Flow channel (30) and flow cell compartment (32) are found among Fig. 2.Flow channel comprises by chamber frame (12) to small part and extends and be at least partially contained within inflow pipe (34) in the chamber frame (12), and inflow pipe (34) is connected on the vertical inflow pipe (36) that is at least partially contained within the chamber frame (12) and is extended by chamber frame (12) to small part.Vertical inflow pipe (36) is communicated with flow cell compartment (32) fluid; Flow cell compartment (32) be at least partially contained within the chamber frame (12) and vertical effuser (38) fluid that is extended to small part by chamber frame (12) is communicated with; Vertical effuser (38) is connected to by chamber frame (12) to small part and extends and be at least partially contained within on the effuser (40) in the chamber frame (12).
Importantly realize the laminar flow of thinning agent in flow cell compartment (32).In one embodiment, this can realize that described little ditch slotted vane (16) comprises a pair of relative shallow fluid groove that forms by methods such as etchings on the surface of little ditch slotted vane (16) by utilizing little ditch slotted vane (16).In one embodiment, shallow fluid groove is the T type, and they are spaced apart from each other and are configured such that the bottom of the shallow fluid groove of T type axially aligns each other, and the top of the shallow fluid groove of T type is with the setting that concerns parallel to each other.
The shallow fluid groove of little ditch slotted vane (16) also provides structure member for the flow channel (30) that is used for thinning agent.By thinning agent being expelled to an end of inflow pipe (34) such as the injection device of syringe pump, pass inflow pipe (34) and reach vertical inflow pipe (36), pass vertical inflow pipe (36) subsequently.Vertical inflow pipe (36) is extended by the bottom side of chamber frame (12), and extends through fluid bore in the upper gasket (14) and the fluid bore in little ditch slotted vane (16).Fluid bore in little ditch slotted vane (16) is positioned at the end of the shallow fluid groove of T type bottom.
Flow cell compartment (32) is the space that is produced by little ditch slotted vane (16), first pad (18), second pad (22) and microslide (24).Flow cell compartment (32) has four or more a plurality of wall.In one embodiment, the bottom side of little ditch slotted vane (16), first pad (18) are formed four walls with the hole internal edge of second pad (22) and the top side of microslide (24).
Matrix (20) is intended to be positioned at flow cell compartment (32), but suspended, and makes not all walls of chamber, contact flow pond (32) of matrix (20).In one embodiment, matrix remains between first pad (18) and second pad (22) by two diverse location places on first pad (18) and second pad (22) internal edge and suspends, thereby pass the hole, but do not touch the bottom of little ditch slotted vane (16) or the top of microslide (24).
Thinning agent is upward extended in the flow cell compartment (32) by vertical inflow pipe (36) at flow channel (30) by the shallow fluid groove of little ditch slotted vane (16).Thinning agent flows through matrix (20) subsequently and leaves flow cell compartment (32) by the shallow fluid groove of the relative positioning of little ditch slotted vane (16), comprises fluid bore in little ditch slotted vane (16), and it is positioned at the end of the shallow fluid groove of the T type bottom of relative positioning.Flow channel (30) is extended to the end of vertical effuser (38) by the fluid bore in little ditch slotted vane (16), described vertical effuser (38) is extended by the bottom side of chamber frame (12), and extends through fluid bore in little ditch slotted vane (16) and the fluid bore in the upper gasket (14).Thinning agent passes vertical effuser (38) to effuser (40), passes effuser (40) subsequently then to leave flow cell (10).
Flow cell compartment (32) should have about 0.05mL to about 100mL, and preferably about 0.1mL is about 50mL extremely, and the 0.1mL fluid volume capacity of about 10mL extremely most preferably from about.Find that these scopes promote laminar flow and are provided for observing the stable environment (laminar flow) of particle deposition.The volume of flow cell compartment (32) will be subjected to the influence of the central hole size of the thickness of first pad (18) and second pad (22) and pad (18,22).
Washer/spacer thickness
The fluid volume capacity of flow cell compartment (32) is adjustable and selected, makes that the space between matrix (20) and little ditch slotted vane (16) and microslide (24) surface can not make matrix (20) touch the plural wall of flow cell compartment (32).First pad (18) and second pad (22) should have separately at least about 0.1mm, more preferably at least about 0.25mm, and most preferably at least about the thickness of 0.50mm.In addition, first pad (18) and second pad (22) should have separately less than about 1.5mm, preferably less than about 1.0mm, and most preferably less than the thickness of about 0.50mm.Flow cell compartment (32) also should have generally approaching or equal to enter the fluid volume capacity of the diluent volume in the flow cell compartment (32) to promote laminar flow as mentioned below.
Flow
Flow in the flow cell (10), the diluent mass flow in the chamber, preferred flow pond (32) should remain about 0.1mL/min to about 0.5mL/min, 0.2mL/min about 0.4mL/min extremely more preferably from about, and 0.2mL/min about 0.3mL/min extremely most preferably from about.
Flow is subjected to inflow pipe (34), vertical inflow pipe (36), vertical effuser (38) and the internal diameter of effuser (40) and the influence of length.Any dead volume (for example those that are produced by the one-tenth angular direction in the aforementioned pipeline) also should be considered into.
In one embodiment, inflow pipe (34) has identical length and internal diameter with effuser (40).The pipe range of inflow pipe (34) and effuser (40) can be about 20mm to about 40mm, and more preferably from about 25mm is to about 35mm, most preferably from about 29mm.The internal diameter of inflow pipe (34) and effuser (40) can be 0.5mm to about 2mm, and more preferably from about 1mm is to about 1.6mm, most preferably from about 1.57mm.
In one embodiment, vertical inflow pipe (36) has identical length and internal diameter with vertical effuser (38).The pipe range of vertical inflow pipe (36) and vertical effuser (38) can be about 5mm to about 20mm, and preferably about 7mm is to about 15mm, preferably about 10mm.The internal diameter of vertical inflow pipe (36) and vertical effuser (38) can be 0.5mm to about 1mm, and preferably about 0.6mm is to about 0.9mm, preferably about 0.88mm.
Optional step
This paper method also can comprise the step of water injection in the flow cell (10), and described injection system is identical with mode in thinning agent being expelled to flow cell (10).The step of injection water also repeats the consumer's " in the use " situation, and can be equal to personal care composition is rinsed out from matrix.The discharge that flows in the flow cell (10) should remain about 0.1mL/min to about 0.5mL/min, 0.2mL/min about 0.4mL/min extremely more preferably from about, and 0.2mL/min about 0.3mL/min extremely most preferably from about.
Recording step
The record of the visual pattern of flow cell compartment should comprise the visual pattern of at least a portion of matrix.Record can be realized described device such as digital camera or video camera by any device that is used for static state or dynamic vision record.In order to help the capture of visual pattern, should utilize light source to make light pass through flow cell (10), preferably to aim at by flow cell (10) from the back up pad (28) of flow cell (10) by light source, pen recorder is positioned on the chamber frame (12) of flow cell (10).Therefore, described method also comprises the step that is illuminated described flow cell by light source, and to pen recorder, described pen recorder is positioned at the opposite side of the described light source of distance of described flow cell to described light source by described flow cell.Capture is used for the example of the visual pattern of described method can be referring to Fig. 3 A-4B.
The image of Fig. 3 A for during said method, taking, wherein the personal care composition such as shampoo Compositions is shown in the following table 1, and described composition is with 1: 9 the shampoo Compositions and the ratio dilute with water of water.Thinning agent is with the flow injection of 0.2mL/min.
The image of Fig. 3 B for being used in description taking during the method for Fig. 3 A, wherein said method comprises the optional step of injecting deionized water, wherein said water is with the flow injection of 0.3mL/min.
The image of Fig. 4 A for taking during the flow cell unit operations of this paper in the presence of the shampoo Compositions that comprises particle and deposition aid shown in the following table 2, described composition is with 1: 9 the shampoo Compositions and the ratio dilute with water of water.Thinning agent is with the flow injection of 0.2mL/min.
Fig. 4 B is at the image of taking during the flow cell unit operations of this paper after the shampoo Compositions that comprises particle and deposition aid of flow cell unit upper punch map brushing 4A with deionized water, and wherein said water is with the flow injection of 0.3mL/min.
Along with thinning agent being imported in the flow cell compartment (32), can in Fig. 4 A, see around and deposit to adjuvant on the single hair fibre substrate (20), and in Fig. 3 A, see seldom or can't see the coacervate particle as the coacervate particle aggregation.Method as herein described provides the clear vision indication of the particle deposition efficient of personal care composition.
In addition, in " in the use " flushing situation when repeating deionized water through flow cell compartment (32), the particle that Fig. 4 B shows the shampoo thinning agent keeps depositing on the hair fiber matrix.Therefore, method as herein described is used to observe and analyze the particle deposition efficient that deposits on the matrix.Flow cell compartment (32) volume that is important to note that this paper in addition selects to provide the clear view of particle deposition, and described deposition is not only along the edge of matrix, but also makes that the particle deposition on whole visible surfaces of hair fiber is visual.
Make it possible to increase visual sedimentary province by utilizing first pad (18) and second pad (22) to increase flow cell compartment (32) volume, the described volume increase matrix (20) that suspends effectively avoids contacting little ditch slotted vane (16) and microslide (24).After the analysis in flow cell (10), matrix (20) can be taken out any required subsequent analysis that is used for such as electron microscope method from flow cell (10), deposit with the adjuvant on the quantitative confirmation matrix (20).
Table 1
1.Poly?LR-30M?ex?Americol
Table 2
1.
AT-1?ex?Rhodia
Dimension disclosed herein and value should be interpreted as that the strictness to quoting exact value limits.On the contrary, except as otherwise noted, each such dimension is intended to represent the value of being quoted and centers on the scope that is equal on this value function.For example, disclosed dimension " 40mm " is intended to expression " about 40mm ".
Unless point out to be not included in interior clearly or in other words restriction, every piece of document that this paper quoted comprises any cross reference or relevant patent or patented claim, all incorporates this paper in full into way of reference hereby.The quoting of any document is not it as disclosed herein or be subjected to the approval of the prior art of the claimed any invention of right; perhaps to himself; perhaps with any other any combination of list of references, perhaps with reference to, propose, suggestion or disclose the approval of any this type of invention.In addition, when any implication of same term in any implication of term in the presents or definition and the file of incorporating into way of reference or when defining contradiction, should obey the implication or the definition of giving described term in the present invention.
Although illustrated and described the present invention with specific embodiments, those be it will be apparent to those skilled in the art that under the situation that does not deviate from the spirit and scope of the invention, can make many other change and modification.Therefore, this means and in claims, comprised all such changes and modifications that belong in the scope of the invention.
Claims (10)
1. one kind is used to make adjuvant deposition visualization method, said method comprising the steps of:
(a) preparation comprises the thinning agent of personal care composition and water, and described personal care composition comprises adjuvant;
(b) matrix is placed flow cell, described flow cell comprises flow cell compartment and flow channel, and described flow cell compartment comprises fluid volume capacity and four or more a plurality of flow cell locular wall, preferably four flow cell locular walls; Described matrix is suspended in the described flow cell compartment, makes described matrix not contact plural described flow cell locular wall and described matrix is positioned at described flow channel;
(c) described thinning agent is expelled in the described flow cell;
(d) visual pattern of the described flow cell compartment of record;
(e) randomly, with water injection in described flow cell.
2. the method for claim 1, the personal care composition that wherein said thinning agent comprises and the dilution ratio of water are 1: 1 to 1: 150, more preferably 1: 2 to 1: 50, and most preferably 1: 2 to 1: 9.
3. the method for claim 1, wherein when described flow channel was positioned at described flow cell compartment, described flow channel caused laminar flow.
4. the method for claim 1, the injection of wherein said thinning agent, the preferred injection that keeps described thinning agent, make that the diluent mass flow that flows in the described flow cell compartment is 0.1mL/min to 0.5mL/min, more preferably 0.2mL/min to 0.4mL/min, and 0.2mL/min to 0.3mL/min most preferably.
5. the method for claim 1, wherein said fluid volume capacity is 0.05mL to 100mL, preferred 0.1mL to 50mL, and 0.1mL to 10mL most preferably.
6. the method for claim 1, the injection that wherein keeps described water, the diluent mass flow that make to flow in the described flow cell compartment is 0.1mL/min to 0.5mL/min, more preferably 0.2mL/min to 0.4mL/min, and 0.2mL/min to 0.3mL/min most preferably.
7. the method for claim 1, wherein the fluid volume capacity is adjustable and selected, make the distance between described matrix and the flow cell locular wall that does not contact described matrix be 0.1mm at least, more preferably 0.25mm at least, and 0.50mm at least most preferably, and less than 1.5mm, preferably less than 1.0mm, and most preferably less than 0.50mm.
8. method as claimed in claim 2, wherein said matrix are hair, and described personal care composition is selected from:
(a) shampoo Compositions, and described dilution ratio is 1: 2 to 1: 10, for example 1: 9, and flow is 0.1 to 0.5mL/min; Or
(b) conditioner composition, and described dilution ratio is 1: 70 to 1: 150, and flow is 0.1 to 0.5mL/min.
9. method as claimed in claim 2, wherein said matrix are bionics skin, and described personal care composition is skin care compositions, and described dilution ratio is 1: 1 to 1: 10, for example 1: 2 or 1: 9, and flow is 0.1 to 0.5mL/min.
10. method as claimed in claim 2, wherein said matrix are fabric, and described personal care composition is Fabrid care composition, and described dilution ratio is 1: 1 to 1: 100, and flow is 0.1 to 0.5mL/min.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8143908P | 2008-07-17 | 2008-07-17 | |
| US61/081,439 | 2008-07-17 | ||
| PCT/US2009/050823 WO2010009301A1 (en) | 2008-07-17 | 2009-07-16 | Method of using a flow cell apparatus for visualizing additive deposition on a substrate |
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| CN102099691A true CN102099691A (en) | 2011-06-15 |
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| EP (1) | EP2300833A1 (en) |
| JP (1) | JP5175395B2 (en) |
| CN (1) | CN102099691A (en) |
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| CN109791797A (en) * | 2016-12-05 | 2019-05-21 | 智慧芽信息科技(苏州)有限公司 | According to the systems, devices and methods of chemical structure similarity searching and display available information in large database concept |
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| JP2018200458A (en) * | 2017-05-29 | 2018-12-20 | 学校法人早稲田大学 | Container for microscopic observation |
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| US20060044554A1 (en) * | 2004-08-26 | 2006-03-02 | Mertz Edward L | Flow-through, thermal-expansion-compensated cell for light spectroscopy |
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| JP2526038B2 (en) * | 1986-06-17 | 1996-08-21 | 株式会社資生堂 | Method and apparatus for measuring hair flexibility |
| US4974952A (en) * | 1988-03-31 | 1990-12-04 | Focht Daniel C | Live cell chamber for microscopes |
| US5414556A (en) * | 1993-03-29 | 1995-05-09 | Focht; Daniel C. | Securing and locking assembly for live cell chambers |
| US20040004717A1 (en) * | 1996-11-13 | 2004-01-08 | Reed Wayne F. | Automatic mixing and dilution methods and apparatus for online characterization of equilibrium and non-equilibrium properties of solutions containing polymers and/or colloids |
| JP3738274B2 (en) * | 1997-06-20 | 2006-01-25 | 株式会社コーセー | Artificial skin model and manufacturing method thereof |
| EP0908174A2 (en) * | 1997-09-18 | 1999-04-14 | INTERNATIONAL FLAVORS & FRAGRANCES INC. | Compositions for fragrancing at least one perfumable material and fragrance diffusion evaluation apparatus |
| DE19835055A1 (en) * | 1998-08-04 | 2000-02-24 | Wella Ag | Method for determining the condition of hair and devices and means suitable therefor |
| DE10056389A1 (en) * | 2000-11-14 | 2002-05-29 | Basf Ag | Automatically producing and characterizing multi-component liquid system comprises automatically producing mixture by combining components in vessel, automatically homogenizing mixture, and automatically evaluating |
| DE10034890A1 (en) * | 2000-07-18 | 2002-01-31 | Basf Ag | Automatically producing and characterizing multi-component liquid system comprises automatically producing mixture by combining components in vessel, automatically homogenizing mixture, and automatically evaluating |
| JP2003004628A (en) * | 2001-06-18 | 2003-01-08 | Shiseido Co Ltd | Cell and method for spectroscopic analysis |
| US6769292B2 (en) * | 2001-08-24 | 2004-08-03 | Symyx Technologies, Inc | High throughput rheological testing of materials |
| JP2004305352A (en) * | 2003-04-04 | 2004-11-04 | Ohm Denki Kk | Hair measuring instrument |
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- 2009-07-16 CN CN2009801277875A patent/CN102099691A/en active Pending
- 2009-07-16 JP JP2011517677A patent/JP5175395B2/en not_active Expired - Fee Related
- 2009-07-16 EP EP09790527A patent/EP2300833A1/en not_active Withdrawn
- 2009-07-16 WO PCT/US2009/050823 patent/WO2010009301A1/en active Application Filing
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| CN1633597A (en) * | 2002-01-09 | 2005-06-29 | 卡钳生命科学股份有限公司 | Slide cassette for fluidic injection |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109791797A (en) * | 2016-12-05 | 2019-05-21 | 智慧芽信息科技(苏州)有限公司 | According to the systems, devices and methods of chemical structure similarity searching and display available information in large database concept |
| CN109791797B (en) * | 2016-12-05 | 2023-05-02 | 智慧芽信息科技(苏州)有限公司 | System, apparatus and method for searching and displaying available information based on chemical structure similarity in large database |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2010009301A1 (en) | 2010-01-21 |
| JP5175395B2 (en) | 2013-04-03 |
| MX2011000580A (en) | 2011-03-02 |
| JP2011527019A (en) | 2011-10-20 |
| EP2300833A1 (en) | 2011-03-30 |
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