CN102058830B

CN102058830B - Medicinal composition preparation and preparation method thereof

Info

Publication number: CN102058830B
Application number: CN2009102375650A
Authority: CN
Inventors: 刘丽颖; 郑松涛
Original assignee: Harbin Pugongying Pharmaceutical Co Ltd
Current assignee: Harbin Pugongying Pharmaceutical Co., Ltd.
Priority date: 2009-11-12
Filing date: 2009-11-12
Publication date: 2013-06-12
Anticipated expiration: 2029-11-12
Also published as: CN102058830A

Abstract

The invention discloses a medicinal composition preparation and a preparation method thereof. The medicinal composition comprises the following raw material medicaments: artificial calculus bovis, pulvis fellis suis, baical skullcap root, tuber of aromatic turmeric, gardenia, borneol, golden thread, pearl, hematite, realgar, cinnabar, menthol crystal, gypsum, cornus bubali concentrated powder and nacre. A preparation process of the medicinal composition comprises the following steps of: grouping the raw material medicaments; preparing intermediate and medicinal powder respectively; mixing; and adding conventional auxiliary materials to prepare a pharmaceutically acceptable formulation by a conventional process. Pharmacodynamic experiments show that the medicinal composition prepared by the method has antipyretic effect, anti-inflammatory effect, antithrombotic effect and pressure reducing effect.

Description

A kind of drug combination preparation and preparation method thereof

Technical field

The present invention relates to a kind of drug combination preparation and preparation method thereof.

Background technology

Annaowan bolus is the prescriptions of Chinese medicine that is recorded in the 13 77 pages of the Sanitation Ministry medicine standard Traditional Chinese medicine historical preparations, standard number is WS3-B-2517-97, and the prescription of having put down in writing Annaowan bolus in standard comprises: artificial Calculus Bovis, Pulvis Fellis Suis, Cinnabaris, Borneolum Syntheticum, Pulvis Cornus Bubali Concentratus, Margarita, Radix Scutellariae, Rhizoma Coptidis, Fructus Gardeniae, Realgar, Radix Curcumae, Gypsum Fibrosum, Haematitum, Concha Margaritifera and Mentholum; The prescription consumption proportion relation of Annaowan bolus that the patent No. has been 93100258.3 Patent Application Publications, and conventional preparation technology; The open Annaowan bolus of available data has heat-clearing and toxic substances removing, refreshment and tranquilization, eliminates phlegm for resuscitation, the relieving convulsion effect that relieves dizziness, high fever, infantile convulsions, epilepsy, etc., and is nowadays hyperpyrexia, the delirious clinical application that a kind for the treatment of acute inflammation commonly used causes.

Yet, Annaowan bolus has existed for many years as the honeyed pill dosage form, by the simple and regular preparation technology that pulverizing, water fly, the step such as refined honey forms can't solve all the time this prescription products and have that potential toxic and side effects, wastage of material are more, dosage form is original causes fully being absorbed by human body, takes the problems such as inconvenient, the problems referred to above exert an influence to the market prospect of this prescription products in the environment that nowadays tradition is write out a prescription and high-tech is combined closely, and simplifying prescription, improveing dosage form, improve preparation technology is all problem demanding prompt solutions that Annaowan bolus faces.

Summary of the invention

The object of the invention is to disclose a kind of drug combination preparation, the present invention also aims to the preparation method of open said preparation.

The present invention seeks to be achieved by the following scheme.

The crude drug of drug combination preparation of the present invention consists of:

Artificial Calculus Bovis 0.5-2 weight portion Pulvis Fellis Suis 7-20 weight portion Rhizoma Coptidis 5-15 weight portion

Radix Scutellariae 5-15 weight portion Margarita 1-5 weight portion Fructus Gardeniae 5-15 weight portion

Radix Curcumae 5-15 weight portion Haematitum 2-6 weight portion Realgar 3-9 weight portion

Cinnabaris 2-6 weight portion Mentholum 0.5-2 weight portion Gypsum Fibrosum 5-12 weight portion

Borneolum Syntheticum 0.5-4 weight portion Pulvis Cornus Bubali Concentratus 7-20 weight portion

Concha Margaritifera 2-8 weight portion.

The crude drug composition of drug combination preparation of the present invention is preferably:

Artificial Calculus Bovis's 1 weight portion Pulvis Fellis Suis 13 weight portion Rhizoma Coptidis 10 weight portions

Radix Scutellariae 10 weight portion Margarita 3 weight portion Fructus Gardeniae 10 weight portions

Radix Curcumae 10 weight portion Haematitum 4 weight portion Realgar 6 weight portions

Cinnabaris 4 weight portion Mentholum 1 weight portion Gypsum Fibrosum 8 weight portions

Borneolum Syntheticum 2 weight portion Pulvis Cornus Bubali Concentratus 13 weight portion Concha Margaritifera 5 weight portions.

Artificial Calculus Bovis's 0.7 weight portion Pulvis Fellis Suis 18 weight portion Rhizoma Coptidis 6 weight portions

Radix Scutellariae 14 weight portion Margarita 2 weight portion Fructus Gardeniae 14 weight portions

Radix Curcumae 6 weight portion Haematitum 5 weight portion Realgar 4 weight portions

Cinnabaris 5 weight portion Mentholum 0.7 weight portion Gypsum Fibrosum 11 weight portions

Borneolum Syntheticum 1 weight portion Pulvis Cornus Bubali Concentratus 18 weight portion Concha Margaritifera 3 weight portions.

Artificial Calculus Bovis's 1.8 weight portion Pulvis Fellis Suis 9 weight portion Rhizoma Coptidis 14 weight portions

Radix Scutellariae 6 weight portion Margarita 4 weight portion Fructus Gardeniae 6 weight portions

Radix Curcumae 14 weight portion Haematitum 3 weight portion Realgar 8 weight portions

Cinnabaris 3 weight portion Mentholum 1.8 weight portion Gypsum Fibrosum 6 weight portions

Borneolum Syntheticum 3.5 weight portion Pulvis Cornus Bubali Concentratus 9 weight portion Concha Margaritifera 7 weight portions.

The preparation method of drug combination preparation of the present invention is following method:

Technique 1: formed by following steps A, B, C, D and E

Steps A: preparation intermediate compound I

A1: with the common heating water reflux, extract, of crude drug Radix Scutellariae, Rhizoma Coptidis, Fructus Gardeniae, Radix Curcumae and Gypsum Fibrosum 2-4 time, each 0.5-2.5 hour, merge decocting liquid, be concentrated into thick paste; The 30-80% ethanol that adds the 4-10 times of weight in thick paste, standing 12-48 hour, get supernatant and reclaim ethanol, concentrated, namely get intermediate compound I; Or after drying the powder of intermediate compound I;

Or A2: with the common heating water reflux, extract, of crude drug Radix Scutellariae, Rhizoma Coptidis, Fructus Gardeniae, Radix Curcumae and Gypsum Fibrosum 2-4 time, each 0.5-2.5 hour, merge decocting liquid, be concentrated into relative density 1-1.0～1.4, centrifugal, supernatant is crossed macroporous resin column, the 30-80% ethanol elution, reclaim ethanol, concentrated, namely get intermediate compound I; Or after drying the powder of intermediate compound I;

Or A3: with the first heating water reflux, extract, of Radix Curcumae 2-4 time, 0.5-2.5 hour at every turn, volatile oil was standby; Radix Curcumae medicinal residues and the common decocting of crude drug Radix Scutellariae, Rhizoma Coptidis, Gypsum Fibrosum and Fructus Gardeniae boil 2-4 time, and each 0.5-2.5 hour, merge decocting liquid, be concentrated into thick paste; The 30%-80% ethanol that adds the 4-10 times of weight in thick paste, standing 12-48 hour, get supernatant and reclaim ethanol, concentrated, spray into volatile oil, namely get intermediate compound I; Or after drying the powder of intermediate compound I;

Or A4: with the first heating water reflux, extract, of Radix Curcumae 2-4 time, 0.5-2.5 hour at every turn, volatile oil was standby; Radix Curcumae medicinal residues and the common decocting of crude drug Radix Scutellariae, Rhizoma Coptidis, Gypsum Fibrosum and Fructus Gardeniae boil 2-4 time, each 0.5-2.5 hour, merge decocting liquid, be concentrated into relative density 1-1.0～1.4, centrifugal, supernatant is crossed macroporous resin column, the 30-80% ethanol elution reclaims ethanol, and is concentrated, spray into volatile oil, namely get intermediate compound I; Or after drying the powder of intermediate compound I;

Step B: preparation intermediate II

B1: respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, mix; Add the 3-10% dilute hydrochloric acid, consumption is the 5-15 times of weight, makes into suspension, standing 12-48 hour, to filter, and filtrate transfers pH value to 3-5 with 10-20% sodium hydroxide (or other alkali liquor); Filter, filtrate is concentrated into thick paste, adds the ethanol of 8-10 times of weight in concentrated solution, and is standing, gets supernatant, reclaims ethanol, concentrated, namely gets intermediate II; Or after drying the powder of intermediate II;

Or B2: respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, mix; Add the 3-10% dilute hydrochloric acid, consumption is the 5-15 times of weight, makes into suspension, standing 12-48 hour, to filter, and filtrate transfers pH value to 3-5 with 10-20% sodium hydroxide (or other alkali liquor); Filter, filtrate is concentrated into thick paste, adds the ethanol of 8-10 times of weight in concentrated solution, and is standing, gets supernatant, reclaim ethanol, be concentrated into relative density 1-1.0～1.4, centrifugal, supernatant is crossed macroporous resin column, the 30-80% ethanol elution reclaims ethanol, and is concentrated, namely gets intermediate II; Or after drying the powder of intermediate II;

Or B3: respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, add respectively the 3-10% dilute hydrochloric acid, consumption is the 5-15 times of weight, make into suspension, standing 12-48 hour, to filter, filtrate transfers pH value to 3-5 with 10-20% sodium hydroxide (or other alkali liquor); Filter, filtrate is concentrated into thick paste, adds the ethanol of 8-10 times of weight in concentrated solution, and is standing, gets supernatant, reclaims ethanol, concentrated, gets respectively the extract of Pulvis Cornus Bubali Concentratus powder, artificial Calculus Bovis, Margarita and Concha Margaritifera, namely gets intermediate II after mixing; Or after combination drying the powder of intermediate II;

Or B4: respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder,, add respectively the 3-10% dilute hydrochloric acid, consumption is the 5-15 times of weight, makes into suspension, standing 12-48 hour, filter, filtrate transfers pH value to 3-5 with 10-20% sodium hydroxide (or other alkali liquor); Filter, filtrate is concentrated into thick paste, adds the ethanol of 8-10 times of weight in concentrated solution, standing, get supernatant, reclaim ethanol, be concentrated into relative density 1-1.0～1.4, centrifugal, supernatant is crossed macroporous resin column, the 30-80% ethanol elution, reclaim ethanol, concentrate, get respectively the extract of Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera, namely get intermediate II after mixing; Or after combination drying the powder of intermediate II;

Step C: preparation intermediate III

C1: get Pulvis Fellis Suis and Realgar is broken into fine powder, mix, add 3-5% sodium hydroxide 4-8 times of weight, standing 12-48 hour, to filter, filtrate transfers pH value to 3-5, and is concentrated, adds 30-80% ethanol 8-15 times of weight; Get supernatant, reclaim ethanol, concentrated, namely get intermediate III; Or after drying the powder of intermediate III;

Or C2: get Pulvis Fellis Suis and be broken into fine powder, add 3-5% sodium hydroxide 4-8 times of weight, standing 12-48 hour, to filter, filtrate transfers pH value to 3-5, and is concentrated, adds 30-80% ethanol 8-15 times of weight; Get supernatant, reclaim ethanol, concentrated, namely get ketone ibuprofen extract, or get the ketone ibuprofen extract powder after drying; Get Realgar powder and be broken into fine powder, add 4-8 times of weight 5-10% hydrochloric acid, standing 8-24 hour, filter, filtrate transfers pH value to 3-5, and is concentrated, adds to be washed to colourlessly, namely gets Realgar extract, or after drying the Realgar extract powder; Ketone ibuprofen extract is mixed with Realgar extract, get intermediate III or the powder of intermediate III;

Step D: preparation medicated powder

D1: respectively Borneolum Syntheticum, Mentholum, Cinnabaris and Haematitum are prepared impalpable powder, be mixed to get medicated powder;

Or D2: Borneolum Syntheticum, Mentholum and Haematitum are prepared impalpable powder, Cinnabaris is added 5-8 times of weight water grind to pasty state, then by 1: 60--70 adds entry and stirs, and standing, inclining suspension, and sediment grinds again; As above method is 3-6 time repeatedly, merges each time suspension, and is standing, gets to be deposited in 25-45 ℃ of airing, and levigation namely gets the Cinnabaris impalpable powder; The impalpable powder of above-mentioned four kinds of crude drug is mixed to get medicated powder;

Step e: preparation

The powder of intermediate compound I, II, III or intermediate compound I, II, III is mixed with medicated powder, technique routinely, add conventional adjuvant to make pharmaceutically acceptable dosage form, as powder, granule, tablet, capsule, dispersible tablet, drop pill, watered pill, honey pill agent, pellet, concentrated pill, soft capsule, slow releasing agent, oral liquid or injection.

Wherein steps A is preferably as follows method:

A1: with the common heating water reflux, extract, of crude drug Radix Scutellariae, Rhizoma Coptidis, Fructus Gardeniae, Radix Curcumae and Gypsum Fibrosum 3 times, each 1.5 hours, merge decocting liquid, be concentrated into thick paste; 50% ethanol that adds 7 times of weight in thick paste, standing 24 hours, get supernatant and reclaim ethanol, concentrated, namely get intermediate compound I; Or after drying the powder of intermediate compound I;

Or A2: with the common heating water reflux, extract, of crude drug Radix Scutellariae, Rhizoma Coptidis, Fructus Gardeniae, Radix Curcumae and Gypsum Fibrosum 3 times, each 1.5 hours, merge decocting liquid, be concentrated into relative density 1-1.2, centrifugal, supernatant is crossed macroporous resin column, 50% ethanol elution, reclaim ethanol, concentrated, namely get intermediate compound I; Or after drying the powder of intermediate compound I;

Or A3: with the first heating water reflux, extract, of Radix Curcumae 3 times, each 1.5 hours, volatile oil was standby; Radix Curcumae medicinal residues and the common decocting of crude drug Radix Scutellariae, Rhizoma Coptidis, Gypsum Fibrosum and Fructus Gardeniae boil 3 times, and each 1.5 hours, merge decocting liquid, be concentrated into thick paste; 50% ethanol that adds 7 times of weight in thick paste, standing 24 hours, get supernatant and reclaim ethanol, concentrated, spray into volatile oil, namely get intermediate compound I; Or after drying the powder of intermediate compound I;

Or A4: with the first heating water reflux, extract, of Radix Curcumae 3 times, each 1.5 hours, volatile oil was standby; Radix Curcumae medicinal residues and the common decocting of crude drug Radix Scutellariae, Rhizoma Coptidis, Gypsum Fibrosum and Fructus Gardeniae boil 3 times, and each 1.5 hours, merge decocting liquid, be concentrated into relative density 1-1.2, centrifugal, supernatant is crossed macroporous resin column, and 50% ethanol elution reclaims ethanol, concentrated, spray into volatile oil, namely get intermediate compound I; Or after drying the powder of intermediate compound I.

Wherein step B is preferably as follows method:

B1: respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, mix; Add 6% dilute hydrochloric acid, consumption is 10 times of weight, makes into suspension, standing 24 hours, to filter, and filtrate is transferred pH value to 4 with 15% sodium hydroxide (or other alkali liquor); Filter, filtrate is concentrated into thick paste, adds the ethanol of 9 times of weight in concentrated solution, and is standing, gets supernatant, reclaims ethanol, concentrated, namely gets intermediate II; Or after drying the powder of intermediate II;

Or B2: respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, mix; Add 6% dilute hydrochloric acid, consumption is 10 times of weight, makes into suspension, standing 24 hours, to filter, and filtrate is transferred pH value to 4 with 15% sodium hydroxide (or other alkali liquor); Filter, filtrate is concentrated into thick paste, adds the ethanol of 9 times of weight in concentrated solution, and is standing, gets supernatant, reclaims ethanol, is concentrated into relative density 1-1.2, and centrifugal, supernatant is crossed macroporous resin column, and 50% ethanol elution reclaims ethanol, and is concentrated, namely gets intermediate II; Or after drying the powder of intermediate II;

Or B3: respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, add respectively 6% dilute hydrochloric acid, consumption is 10 times of weight, make into suspension, standing 24 hours, to filter, filtrate is transferred pH value to 4 with 15% sodium hydroxide (or other alkali liquor); Filter, filtrate is concentrated into thick paste, adds the ethanol of 9 times of weight in concentrated solution, and is standing, gets supernatant, reclaims ethanol, concentrated, gets respectively the extract of Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera, namely gets intermediate II after mixing; Or after combination drying the powder of intermediate II;

Or B4: respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, add respectively 6% dilute hydrochloric acid, consumption is 10 times of weight, make into suspension, standing 24 hours, to filter, filtrate is transferred pH value to 4 with 15% sodium hydroxide (or other alkali liquor); Filter, filtrate is concentrated into thick paste, adds the ethanol of 9 times of weight in concentrated solution, standing, get supernatant, reclaim ethanol, be concentrated into relative density 1-1.2, centrifugal, supernatant is crossed macroporous resin column, 50% ethanol elution, reclaim ethanol, concentrate, get respectively the extract of Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera, namely get intermediate II after mixing; Or after combination drying the powder of intermediate II.

Wherein step C is preferably as follows method:

C1: get Pulvis Fellis Suis and Realgar is broken into fine powder, mix, add 4% sodium hydroxide 6 times of weight, standing 24 hours, to filter, filtrate is transferred pH value to 4, and is concentrated, adds 50% ethanol 12 times of weight; Get supernatant, reclaim ethanol, concentrated, namely get intermediate III; Or after drying the powder of intermediate III;

Or C2: get Pulvis Fellis Suis and be broken into fine powder, add 4% sodium hydroxide 6 times of weight, standing 24 hours, to filter, filtrate is transferred pH value to 4, and is concentrated, adds 50% ethanol 12 times of weight; Get supernatant, reclaim ethanol, concentrated, namely get ketone ibuprofen extract, or get the ketone ibuprofen extract powder after drying; Get Realgar powder and be broken into fine powder, add 6 times of weight 8% hydrochloric acid, standing 24 hours, filter, filtrate is transferred pH value to 4, and is concentrated, adds to be washed to colourlessly, namely gets Realgar extract, or after drying the Realgar extract powder; Ketone ibuprofen extract is mixed with Realgar extract, get intermediate III or the powder of intermediate III.

Wherein step D is preferably as follows method:

Or D2: Borneolum Syntheticum, Mentholum and Haematitum are prepared impalpable powder, Cinnabaris is added 6 times of weight water grind to pasty state, then added the entry stirring by 1: 65, standing, inclining suspension, and sediment grinds again; As above method is 4 times repeatedly, merges each time suspension, and is standing, gets and is deposited in 35 ℃ of airings, and levigation namely gets the Cinnabaris impalpable powder; The impalpable powder of above-mentioned four kinds of crude drug is mixed to get medicated powder.

Wherein, above-mentioned any step all can be substituted by conventional method.

Wherein, above-mentioned macroporous resin column model is: YPR-II, X5, AB-8, HPD-100, DX-5, D101, DA201, DM130, WLD-3 or NKA-9.

The preparation method of drug combination preparation of the present invention can also be following method:

Technique 2: formed by following steps A, B, C and D

Steps A: preparation intermediate compound I

Step B: preparation intermediate II

B1: crude drug Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita, Concha Margaritifera, Pulvis Fellis Suis and Realgar powder are broken into fine powder, mix, add the 30-80% ethanol of 4-10 times of weight, 40-60 ℃ warm macerating 12-48 hour, filter, get filtrate, reclaim ethanol, concentrated, namely get intermediate II; Or after drying the powder of intermediate II;

Step C: preparation medicated powder

C1: respectively Borneolum Syntheticum, Mentholum, Cinnabaris and Haematitum are prepared impalpable powder, be mixed to get medicated powder;

Or C2: Borneolum Syntheticum, Mentholum and Haematitum are prepared impalpable powder, Cinnabaris is added 5-8 times of weight water grind to pasty state, then by 1: 60--70 adds entry and stirs, and standing, inclining suspension, and sediment grinds again; As above method is 3-6 time repeatedly, merges each time suspension, and is standing, gets to be deposited in 25-45 ℃ of airing, and levigation namely gets the Cinnabaris impalpable powder; The impalpable powder of above-mentioned four kinds of crude drug is mixed to get medicated powder;

Step D: preparation

The powder of intermediate compound I, II or intermediate compound I, II is mixed with medicated powder, technique routinely, add conventional adjuvant to make pharmaceutically acceptable dosage form, as powder, granule, tablet, capsule, dispersible tablet, drop pill, watered pill, honey pill agent, pellet, concentrated pill, soft capsule, slow releasing agent, oral liquid or injection.

Wherein steps A is preferably as follows method:

Wherein step B is preferably as follows method:

B1: crude drug Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita, Concha Margaritifera, Pulvis Fellis Suis and Realgar powder are broken into fine powder, mix, add 50% ethanol of 7 times of weight, 50 ℃ of warm macerating 24 hours filter, and get filtrate, reclaim ethanol, and are concentrated, namely get intermediate II; Or after drying the powder of intermediate II.

Wherein step C is preferably as follows method:

C1: respectively Borneolum Syntheticum, Mentholum, Cinnabaris and Haematitum are prepared impalpable powder, respectively or be mixed to get medicated powder;

Or C2: Borneolum Syntheticum, Mentholum and Haematitum are prepared impalpable powder, Cinnabaris is added 6 times of weight water grind to pasty state, then added the entry stirring by 1: 65, standing, inclining suspension, and sediment grinds again; As above method is 4 times repeatedly, merges each time suspension, and is standing, gets and is deposited in 35 ℃ of airings, and levigation namely gets the Cinnabaris impalpable powder; The impalpable powder of above-mentioned four kinds of crude drug is mixed to get medicated powder.

By pharmacodynamic experiment, that the pharmaceutical composition that shows the method for the invention preparation has is analgesic, antiinflammatory, antithrombotic forms and the effect of blood pressure lowering.

Following experimental example and embodiment are used for further illustrating but being not limited to the present invention.

The medicinal composition powders of the present invention that the experimental example 1 described extracting method of embodiment of the present invention 1-48 is made is on the hypertensive impact of rat experiment

Laboratory animal: healthy male Waster rat, body weight 190g～210g;

Experiment equipment: RBP-1 type rat blood pressure meter;

Experimental drug and reagent: modeling agent: androlin; Get the medicinal composition powders of the present invention that the described extracting method of embodiment of the present invention 1-48 is made, be mixed with 100mg/ml with normal saline, 4-5 ℃ of preservation.

Experimental technique: get healthy rat, be divided at random 28 groups, 8 every group, survey normotensive value and continuous measurement 3 days.After METHOD FOR CONTINUOUS DETERMINATION 3 days, subcutaneous injection androlin 25mg/kg/ day, each group gavages respectively the powder that the different preparation methoies of equivalent (1g/kg) the present invention are made, continuous 10 days.Measure animal blood pressure on time, observe its blood pressure, the results are shown in Table 1.

The pharmaceutical composition of table 1 the inventive method preparation is on the hypertensive impact of rat test (n=8, mmHg, X ± S)

Table 1 shows that in embodiment 1-48, optimum extracting method is embodiment 23,24,35,36, the described scheme of 41-48.

Analgesic and the antiinflammatory action of the medicinal composition powders of the present invention that the experimental example 2 described extracting method of embodiment of the present invention 1-48 are made to the inflammatory rat

Laboratory animal: healthy male Waster rat, body weight 190g～210g;

Experiment equipment: pointer electron temperature indicator.

Pyrogen: 1% carrageenin.

Experimental technique: take carrageenin 100mg, add sterile saline 10ml, mixing, in 4 ℃ of refrigerators, take out morning next day, is inverted to make suspension even for several times, standby; Get the medicinal composition powders of the present invention that the described extracting method of embodiment of the present invention 1-48 is made, be mixed with 100mg/ml with normal saline respectively, 4 ℃ save backup; Healthy rat every day is surveyed axil temperature 2 times (upper and lower noon each 1 time) with the electronics temperature indicator, continuous 3 days, chooses the body temperature rat that is no more than 0.3 ℃ of fluctuating and is divided at random 28 groups, 10 every group.Every group gavages the powder 1g/kg that the different preparation methoies of the present invention are made, and after this administration measures its axil temperature simultaneously in rat rear foot ripple plantar subcutaneous injection 1% carrageenin suspension 0.1ml/ pawl on time, the results are shown in Table 2.

The impact of rat fever due to the pharmaceutical composition on Carrageenan of table 2 distinct methods preparation of the present invention (℃, X ± S)

Table 2 shows that in embodiment 1-48, the best approach is embodiment 23,24,35,36, the described scheme of 41-48.

Following embodiment all can realize the described effect of above-mentioned experimental example.

The specific embodiment

Crude drug described in following embodiment forms 1:

Artificial Calculus Bovis 1kg Pulvis Fellis Suis 13kg Rhizoma Coptidis 10kg

Radix Scutellariae 10kg Margarita 3kg Fructus Gardeniae 10kg

Radix Curcumae 10kg Haematitum 4kg Realgar 6kg

Cinnabaris 4kg Mentholum 1kg Gypsum Fibrosum 8kg

Borneolum Syntheticum 2kg Pulvis Cornus Bubali Concentratus 13kg Concha Margaritifera 5kg.

Described crude drug forms 2:

Artificial Calculus Bovis 0.7kg Pulvis Fellis Suis 18kg Rhizoma Coptidis 6kg

Radix Scutellariae 14kg Margarita 2kg Fructus Gardeniae 14kg

Radix Curcumae 6kg Haematitum 5kg Realgar 4kg

Cinnabaris 5kg Mentholum 0.7kg Gypsum Fibrosum 11kg

Borneolum Syntheticum 1kg Pulvis Cornus Bubali Concentratus 18kg Concha Margaritifera 3kg.

Described crude drug forms 3:

Artificial Calculus Bovis 1.8kg Pulvis Fellis Suis 9kg Rhizoma Coptidis 14kg

Radix Scutellariae 6kg Margarita 4kg Fructus Gardeniae 6kg

Radix Curcumae 14kg Haematitum 3kg Realgar 8kg

Cinnabaris 3kg Mentholum 1.8kg Gypsum Fibrosum 6kg

Borneolum Syntheticum 3.5kg Pulvis Cornus Bubali Concentratus 9kg Concha Margaritifera 7kg.

Described technique 1 steps A 1 is:

A1: with the common heating water reflux, extract, of crude drug Radix Scutellariae, Rhizoma Coptidis, Fructus Gardeniae, Radix Curcumae and Gypsum Fibrosum 3 times, each 1.5 hours, merge decocting liquid, be concentrated into thick paste; 50% ethanol that adds 7 times of weight in thick paste, standing 24 hours, get supernatant and reclaim ethanol, concentrate, get the powder of intermediate compound I after drying.

Described technique 1 steps A 2 is:

With the common heating water reflux, extract, of crude drug Radix Scutellariae, Rhizoma Coptidis, Fructus Gardeniae, Radix Curcumae and Gypsum Fibrosum 3 times, each 1.5 hours, merge decocting liquid, be concentrated into relative density 1-1.2, centrifugal, supernatant is crossed macroporous resin column, 50% ethanol elution, reclaim ethanol, concentrated, namely get intermediate compound I; Or after drying the powder of intermediate compound I.

Described technique 1 steps A 3 is:

With the first heating water reflux, extract, of Radix Curcumae 3 times, each 1.5 hours, volatile oil was standby; Radix Curcumae medicinal residues and the common decocting of crude drug Radix Scutellariae, Rhizoma Coptidis, Gypsum Fibrosum and Fructus Gardeniae boil 3 times, and each 1.5 hours, merge decocting liquid, be concentrated into thick paste; 50% ethanol that adds 7 times of weight in thick paste, standing 24 hours, get supernatant and reclaim ethanol, concentrated, spray into volatile oil, namely get intermediate compound I; Or after drying the powder of intermediate compound I.

Described technique 1 steps A 4 is:

With the first heating water reflux, extract, of Radix Curcumae 3 times, each 1.5 hours, volatile oil was standby; Radix Curcumae medicinal residues and the common decocting of crude drug Radix Scutellariae, Rhizoma Coptidis, Gypsum Fibrosum and Fructus Gardeniae boil 3 times, and each 1.5 hours, merge decocting liquid, be concentrated into relative density 1-1.2, centrifugal, supernatant is crossed macroporous resin column, and 50% ethanol elution reclaims ethanol, concentrated, spray into volatile oil, namely get intermediate compound I; Or after drying the powder of intermediate compound I.

Described technique 1 step B1 is:

Respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, mix; Add 6% dilute hydrochloric acid, consumption is 10 times of weight, makes into suspension, standing 24 hours, to filter, and filtrate is transferred pH value to 4 with 15% sodium hydroxide (or other alkali liquor); Filter, filtrate is concentrated into thick paste, adds the ethanol of 9 times of weight in concentrated solution, and is standing, gets supernatant, reclaims ethanol, concentrated, namely gets intermediate II; Or after drying the powder of intermediate II.

Described technique 1 step B2 is:

Respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, mix; Add 6% dilute hydrochloric acid, consumption is 10 times of weight, makes into suspension, standing 24 hours, to filter, and filtrate is transferred pH value to 4 with 15% sodium hydroxide (or other alkali liquor); Filter, filtrate is concentrated into thick paste, adds the ethanol of 9 times of weight in concentrated solution, and is standing, gets supernatant, reclaims ethanol, is concentrated into relative density 1-1.2, and centrifugal, supernatant is crossed macroporous resin column, and 50% ethanol elution reclaims ethanol, and is concentrated, namely gets intermediate II; Or after drying the powder of intermediate II.

Described technique 1 step B3 is:

Respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, add respectively 6% dilute hydrochloric acid, consumption is 10 times of weight, makes into suspension, standing 24 hours, to filter, and filtrate is transferred pH value to 4 with 15% sodium hydroxide (or other alkali liquor); Filter, filtrate is concentrated into thick paste, adds the ethanol of 9 times of weight in concentrated solution, and is standing, gets supernatant, reclaims ethanol, concentrated, gets respectively the extract of Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera, namely gets intermediate II after mixing; Or after combination drying the powder of intermediate II.

Described technique 1 step B4 is:

Respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, add respectively 6% dilute hydrochloric acid, consumption is 10 times of weight, makes into suspension, standing 24 hours, to filter, and filtrate is transferred pH value to 4 with 15% sodium hydroxide (or other alkali liquor); Filter, filtrate is concentrated into thick paste, adds the ethanol of 9 times of weight in concentrated solution, standing, get supernatant, reclaim ethanol, be concentrated into relative density 1-1.2, centrifugal, supernatant is crossed macroporous resin column, 50% ethanol elution, reclaim ethanol, concentrate, get respectively the extract of Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera, namely get intermediate II after mixing; Or after combination drying the powder of intermediate II.

Described technique 1 step C1 is:

Get Pulvis Fellis Suis and Realgar is broken into fine powder, mix, add 4% sodium hydroxide 6 times of weight, standing 24 hours, to filter, filtrate is transferred pH value to 4, and is concentrated, adds 50% ethanol 12 times of weight; Get supernatant, reclaim ethanol, concentrated, namely get intermediate III; Or after drying the powder of intermediate III.

Described technique 1 step C2 is:

Get Pulvis Fellis Suis and be broken into fine powder, add 4% sodium hydroxide 6 times of weight, standing 24 hours, to filter, filtrate is transferred pH value to 4, and is concentrated, adds 50% ethanol 12 times of weight; Get supernatant, reclaim ethanol, concentrated, namely get ketone ibuprofen extract, or get the ketone ibuprofen extract powder after drying; Get Realgar powder and be broken into fine powder, add 6 times of weight 8% hydrochloric acid, standing 24 hours, filter, filtrate is transferred pH value to 4, and is concentrated, adds to be washed to colourlessly, namely gets Realgar extract, or after drying the Realgar extract powder; Ketone ibuprofen extract is mixed with Realgar extract, get intermediate III or the powder of intermediate III.

Described technique 1 step D1 is:

Respectively Borneolum Syntheticum, Mentholum, Cinnabaris and Haematitum are prepared impalpable powder, be mixed to get medicated powder.

Described technique 1 step D2 is:

Borneolum Syntheticum, Mentholum and Haematitum are prepared impalpable powder, Cinnabaris is added 6 times of weight water grind to pasty state, then added the entry stirring by 1: 65, standing, inclining suspension, and sediment grinds again; As above method is 4 times repeatedly, merges each time suspension, and is standing, gets and is deposited in 35 ℃ of airings, and levigation namely gets the Cinnabaris impalpable powder; The impalpable powder of above-mentioned four kinds of crude drug is mixed to get medicated powder.

Described technique 2 steps A 1 are:

With the common heating water reflux, extract, of crude drug Radix Scutellariae, Rhizoma Coptidis, Fructus Gardeniae, Radix Curcumae and Gypsum Fibrosum 3 times, each 1.5 hours, merge decocting liquid, be concentrated into thick paste; 50% ethanol that adds 7 times of weight in thick paste, standing 24 hours, get supernatant and reclaim ethanol, concentrated, namely get intermediate compound I; Or after drying the powder of intermediate compound I.

Described technique 2 steps A 2 are:

Described technique 2 steps A 3 are:

Described technique 2 steps A 4 are:

Described technique 2 step B1 are:

Crude drug Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita, Concha Margaritifera, Pulvis Fellis Suis and Realgar powder are broken into fine powder, mix, add 50% ethanol of 7 times of weight, 50 ℃ of warm macerating 24 hours filter, and get filtrate, reclaim ethanol, and are concentrated, namely get intermediate II; Or after drying the powder of intermediate II.

Described technique 2 step C1 are:

Respectively Borneolum Syntheticum, Mentholum, Cinnabaris and Haematitum are prepared impalpable powder, respectively or be mixed to get medicated powder.

Described technique 2 step C2 are:

Embodiment 1: powder

Crude drug forms 1

Processing step is steps A 1, B1, C1, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 2: powder

Crude drug forms 2

Processing step is steps A 2, B1, C1, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 3: powder

Crude drug forms 3

Processing step is steps A 3, B1, C1, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 4: granule

Crude drug forms 1

Processing step is steps A 4, B1, C1, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add the agent of conventional adjuvant granulation routinely.

Embodiment 5: granule

Crude drug forms 2

Processing step is steps A 1, B2, C2, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add the agent of conventional adjuvant granulation routinely.

Embodiment 6: granule

Crude drug forms 3

Processing step is steps A 2, B2, C2, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add the agent of conventional adjuvant granulation routinely.

Embodiment 7: tablet

Crude drug forms 1

Processing step is steps A 3, B2, C2, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make tablet routinely.

Embodiment 8: tablet

Crude drug forms 2

Processing step is steps A 4, B2, C2, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make tablet routinely.

Embodiment 9: tablet

Crude drug forms 3

Processing step is steps A 1, B3, C1, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make tablet routinely.

Embodiment 10: capsule

Crude drug forms 1

Processing step is steps A 2, B3, C1, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make capsule routinely.

Embodiment 11: capsule

Crude drug forms 2

Processing step is steps A 3, B3, C1, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make capsule routinely.

Embodiment 12: capsule

Crude drug forms 3

Processing step is steps A 4, B3, C1, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make capsule routinely.

Embodiment 13: dispersible tablet

Crude drug forms 1

Processing step is steps A 1, B4, C2, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make dispersible tablet routinely.

Embodiment 14: dispersible tablet

Crude drug forms 2

Processing step is steps A 2, B4, C2, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make dispersible tablet routinely.

Embodiment 15: dispersible tablet

Crude drug forms 3

Processing step is steps A 3, B4, C2, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make dispersible tablet routinely.

Embodiment 16: drop pill

Crude drug forms 1

Processing step is steps A 4, B4, C2, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make drop pill routinely.

Embodiment 17: drop pill

Crude drug forms 2

Processing step is steps A 1, B1, C2, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make drop pill routinely.

Embodiment 18: drop pill

Crude drug forms 3

Processing step is steps A 2, B1, C2, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make drop pill routinely.

Embodiment 19: the watered pill

Crude drug forms 1

Processing step is steps A 3, B1, C2, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make the watered pill routinely.

Embodiment 20: the watered pill

Crude drug forms 2

Processing step is steps A 4, B1, C2, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make the watered pill routinely.

Embodiment 21: the watered pill

Crude drug forms 3

Processing step is steps A 1, B2, C1, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make the watered pill routinely.

Embodiment 22: honeyed pill

Crude drug forms 1

Processing step is steps A 2, B2, C1, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make honeyed pill routinely.

Embodiment 23: powder

Crude drug forms 1

Processing step is steps A 3, B2, C1, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 24: powder

Crude drug forms 1

Processing step is steps A 4, B2, C1, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 25: honeyed pill

Crude drug forms 1

Processing step is steps A 1, B3, C2, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make honeyed pill routinely.

Embodiment 26: honeyed pill

Crude drug forms 2

Processing step is steps A 2, B3, C2, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make honeyed pill routinely.

Embodiment 27: micropill

Crude drug forms 3

Processing step is steps A 3, B3, C2, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make micropill routinely.

Embodiment 28: micropill

Crude drug forms 1

Processing step is steps A 4, B3, C2, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make micropill routinely.

Embodiment 29: concentrated pill

Crude drug forms 2

Processing step is steps A 1, B4, C1, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make concentrated pill routinely.

Embodiment 30: concentrated pill

Crude drug forms 3

Processing step is steps A 2, B4, C1, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make concentrated pill routinely.

Embodiment 31: soft capsule

Crude drug forms 1

Processing step is steps A 3, B4, C1, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make soft capsule routinely.

Embodiment 32: soft capsule

Crude drug forms 2

Processing step is steps A 4, B4, C1, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make soft capsule routinely.

Embodiment 33: slow releasing agent

Crude drug forms 3

Processing step is steps A 1, B3, C1, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make slow releasing agent routinely.

Embodiment 34: slow releasing agent

Crude drug forms 1

Processing step is steps A 2, B3, C1, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make slow releasing agent routinely.

Embodiment 35: powder

Crude drug forms 1

Processing step is steps A 3, B3, C1, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 36: powder

Crude drug forms 1

Processing step is steps A 4, B3, C1, the D2 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 37: oral liquid

Crude drug forms 1

Processing step is steps A 1, B4, C2, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make oral liquid routinely.

Embodiment 38: oral liquid

Crude drug forms 2

Processing step is steps A 2, B4, C2, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make oral liquid routinely.

Embodiment 39: powder

Crude drug forms 3

Processing step is steps A 3, B4, C2, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 40: powder

Crude drug forms 1

Processing step is steps A 4, B4, C2, the D1 in technique 1, and the powder of intermediate compound I, II, III is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 41: powder

Crude drug forms 1

Processing step is steps A 1, B1, the C1 in technique 2, and the powder of intermediate compound I, II is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 42: powder

Crude drug forms 1

Processing step is steps A 2, B1, the C1 in technique 2, and the powder of intermediate compound I, II is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 43: powder

Crude drug forms 1

Processing step is steps A 3, B1, the C1 in technique 2, and the powder of intermediate compound I, II is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 44: powder

Crude drug forms 1

Processing step is steps A 4, B1, the C1 in technique 2, and the powder of intermediate compound I, II is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 45: powder

Crude drug forms 1

Processing step is steps A 1, B1, the C2 in technique 2, and the powder of intermediate compound I, II is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 46: powder

Crude drug forms 1

Processing step is steps A 2, B1, the C2 in technique 2, and the powder of intermediate compound I, II is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 47: powder

Crude drug forms 1

Processing step is steps A 3, B1, the C2 in technique 2, and the powder of intermediate compound I, II is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Embodiment 48: powder

Crude drug forms 1

Processing step is steps A 4, B1, the C2 in technique 2, and the powder of intermediate compound I, II is mixed with medicated powder, and technique, add conventional adjuvant to make powder routinely.

Claims

1. drug combination preparation, the crude drug of said preparation consists of:

Concha Margaritifera 2-8 weight portion;

It is characterized in that said preparation prepares by the following method:

The preparation intermediate compound I:

With the common heating water reflux, extract, of crude drug Radix Scutellariae, Rhizoma Coptidis, Fructus Gardeniae, Radix Curcumae and Gypsum Fibrosum 2-4 time, each 0.5-2.5 hour, merge decocting liquid, be concentrated into relative density 1-1.2, centrifugal, supernatant is crossed macroporous resin column, the 30-80% ethanol elution, reclaim ethanol, concentrated, namely get intermediate compound I; Or after drying the powder of intermediate compound I;

Or: with the first heating water reflux, extract, of Radix Curcumae 2-4 time, 0.5-2.5 hour at every turn, volatile oil was standby; Radix Curcumae medicinal residues and the common decocting of crude drug Radix Scutellariae, Rhizoma Coptidis, Gypsum Fibrosum and Fructus Gardeniae boil 2-4 time, and each 0.5-2.5 hour, merge decocting liquid, be concentrated into thick paste; The 30%-80% ethanol that adds the 4-10 times of weight in thick paste, standing 12-48 hour, get supernatant and reclaim ethanol, concentrated, spray into volatile oil, namely get intermediate compound I; Or after drying the powder of intermediate compound I;

Or: with the first heating water reflux, extract, of Radix Curcumae 2-4 time, 0.5-2.5 hour at every turn, volatile oil was standby; Radix Curcumae medicinal residues and the common decocting of crude drug Radix Scutellariae, Rhizoma Coptidis, Gypsum Fibrosum and Fructus Gardeniae boil 2-4 time, and each 0.5-2.5 hour, merge decocting liquid, be concentrated into relative density 1-1.2, centrifugal, supernatant is crossed macroporous resin column, and the 30-80% ethanol elution reclaims ethanol, concentrated, spray into volatile oil, namely get intermediate compound I; Or after drying the powder of intermediate compound I;

The preparation intermediate II:

Respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, mix; Add the 3-10% dilute hydrochloric acid, consumption is the 5-15 times of weight, makes into suspension, standing 12-48 hour, to filter, and filtrate uses 10-20% sodium hydroxide or other lye pH adjustment values to 3-5; Filter, filtrate is concentrated into thick paste, adds the ethanol of 8-10 times of weight in concentrated solution, and is standing, gets supernatant, reclaims ethanol, concentrated, namely gets intermediate II; Or after drying the powder of intermediate II;

Or: respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, mix; Add the 3-10% dilute hydrochloric acid, consumption is the 5-15 times of weight, makes into suspension, standing 12-48 hour, to filter, and filtrate uses 10-20% sodium hydroxide or other lye pH adjustment values to 3-5; Filter, filtrate is concentrated into thick paste, adds the ethanol of 8-10 times of weight in concentrated solution, and is standing, gets supernatant, reclaim ethanol, be concentrated into relative density 1-1.2, centrifugal, supernatant is crossed macroporous resin column, the 30-80% ethanol elution reclaims ethanol, and is concentrated, namely gets intermediate II; Or after drying the powder of intermediate II;

Or: respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, add respectively the 3-10% dilute hydrochloric acid, consumption is the 5-15 times of weight, make into suspension, standing 12-48 hour, to filter, filtrate uses 10-20% sodium hydroxide or other lye pH adjustment values to 3-5; Filter, filtrate is concentrated into thick paste, adds the ethanol of 8-10 times of weight in concentrated solution, and is standing, gets supernatant, reclaims ethanol, concentrated, gets respectively the extract of Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera, namely gets intermediate II after mixing; Or after combination drying the powder of intermediate II;

Or: respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, add respectively the 3-10% dilute hydrochloric acid, consumption is the 5-15 times of weight, make into suspension, standing 12-48 hour, to filter, filtrate uses 10-20% sodium hydroxide or other lye pH adjustment values to 3-5; Filter, filtrate is concentrated into thick paste, adds the ethanol of 8-10 times of weight in concentrated solution, standing, get supernatant, reclaim ethanol, be concentrated into relative density 1-1.2, centrifugal, supernatant is crossed macroporous resin column, the 30-80% ethanol elution, reclaim ethanol, concentrate, get respectively the extract of Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera, namely get intermediate II after mixing; Or after combination drying the powder of intermediate II;

The preparation intermediate III:

Get Pulvis Fellis Suis and Realgar is broken into fine powder, mix, add 3-5% sodium hydroxide 4-8 times of weight, standing 12-48 hour, filter, the filtrate adjust pH is to 3-5, and is concentrated, adds 30-80% ethanol 8-15 times of weight; Get supernatant, reclaim ethanol, concentrated, namely get intermediate III; Or after drying the powder of intermediate III;

Or: get Pulvis Fellis Suis and be broken into fine powder, add 3-5% sodium hydroxide 4-8 times of weight, standing 12-48 hour, filter, the filtrate adjust pH is to 3-5, and is concentrated, adds 30-80% ethanol 8-15 times of weight; Get supernatant, reclaim ethanol, concentrated, namely get ketone ibuprofen extract, or get the ketone ibuprofen extract powder after drying; Get Realgar powder and be broken into fine powder, add 4-8 times of weight 5-10% hydrochloric acid, standing 8-24 hour, filter, the filtrate adjust pH is to 3-5, and is concentrated, adds to be washed to colourlessly, namely gets Realgar extract, or after drying the Realgar extract powder; Ketone ibuprofen extract is mixed with Realgar extract, get intermediate III or the powder of intermediate III;

Preparation medicated powder:

Respectively Borneolum Syntheticum, Mentholum, Cinnabaris and Haematitum are prepared impalpable powder, be mixed to get medicated powder;

Or: Borneolum Syntheticum, Mentholum and Haematitum are prepared impalpable powder, Cinnabaris is added 5-8 times of weight water grind to pasty state, then by 1: 60--70 adds entry and stirs, and standing, inclining suspension, and sediment grinds again; As above method is 3-6 time repeatedly, merges each time suspension, and is standing, gets to be deposited in 25-45 ℃ of airing, and levigation namely gets the Cinnabaris impalpable powder; The impalpable powder of above-mentioned four kinds of crude drug is mixed to get medicated powder;

The powder of intermediate compound I, II, III or intermediate compound I, II, III is mixed with medicated powder, technique, add conventional adjuvant to make pharmaceutically acceptable powder, granule, tablet, capsule, drop pill, watered pill, honey pill agent, pellet, concentrated pill, slow releasing agent, oral liquid or injection routinely.

2. drug combination preparation as claimed in claim 1 is characterized in that the method for preparing intermediate II in the preparation method of said preparation is:

Respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, mix; Add 6% dilute hydrochloric acid, consumption is 10 times of weight, makes into suspension, standing 24 hours, to filter, and filtrate is with 15% sodium hydroxide or other lye pH adjustment values to 4; Filter, filtrate is concentrated into thick paste, adds the ethanol of 9 times of weight in concentrated solution, and is standing, gets supernatant, reclaims ethanol, concentrated, namely gets intermediate II; Or after drying the powder of intermediate II;

Or: respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, mix; Add 6% dilute hydrochloric acid, consumption is 10 times of weight, makes into suspension, standing 24 hours, to filter, and filtrate is with 15% sodium hydroxide or other lye pH adjustment values to 4; Filter, filtrate is concentrated into thick paste, adds the ethanol of 9 times of weight in concentrated solution, and is standing, gets supernatant, reclaims ethanol, is concentrated into relative density 1-1.2, and centrifugal, supernatant is crossed macroporous resin column, and 50% ethanol elution reclaims ethanol, and is concentrated, namely gets intermediate II; Or after drying the powder of intermediate II;

Or: respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, add respectively 6% dilute hydrochloric acid, consumption is 10 times of weight, makes into suspension, standing 24 hours, to filter, filtrate is with 15% sodium hydroxide or other lye pH adjustment values to 4; Filter, filtrate is concentrated into thick paste, adds the ethanol of 9 times of weight in concentrated solution, and is standing, gets supernatant, reclaims ethanol, concentrated, gets respectively the extract of Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera, namely gets intermediate II after mixing; Or after combination drying the powder of intermediate II;

Or: respectively Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera are ground into fine powder, add respectively 6% dilute hydrochloric acid, consumption is 10 times of weight, makes into suspension, standing 24 hours, to filter, filtrate is with 15% sodium hydroxide or other lye pH adjustment values to 4; Filter, filtrate is concentrated into thick paste, adds the ethanol of 9 times of weight in concentrated solution, standing, get supernatant, reclaim ethanol, be concentrated into relative density 1-1.2, centrifugal, supernatant is crossed macroporous resin column, 50% ethanol elution, reclaim ethanol, concentrate, get respectively the extract of Pulvis Cornus Bubali Concentratus, artificial Calculus Bovis, Margarita and Concha Margaritifera, namely get intermediate II after mixing; Or after combination drying the powder of intermediate II.

3. drug combination preparation as claimed in claim 1 or 2 is characterized in that the method for preparing intermediate III in the preparation method of said preparation is:

Get Pulvis Fellis Suis and Realgar is broken into fine powder, mix, add 4% sodium hydroxide 6 times of weight, standing 24 hours, filter, filtrate adjust pH to 4, concentrated, add 50% ethanol 12 times of weight; Get supernatant, reclaim ethanol, concentrated, namely get intermediate III; Or after drying the powder of intermediate III;

Or: get Pulvis Fellis Suis and be broken into fine powder, add 4% sodium hydroxide 6 times of weight, standing 24 hours, filter, filtrate adjust pH to 4, concentrated, add 50% ethanol 12 times of weight; Get supernatant, reclaim ethanol, concentrated, namely get ketone ibuprofen extract, or get the ketone ibuprofen extract powder after drying; Get Realgar powder and be broken into fine powder, add 6 times of weight 8% hydrochloric acid, standing 24 hours, filter, filtrate adjust pH to 4, concentrated, add to be washed to colourlessly, namely get Realgar extract, or after drying the Realgar extract powder; Ketone ibuprofen extract is mixed with Realgar extract, get intermediate III or the powder of intermediate III.

4. drug combination preparation as claimed in claim 1 or 2 is characterized in that the preparation method of the preparation method Chinese medicine powder of said preparation is:

Or: Borneolum Syntheticum, Mentholum and Haematitum are prepared impalpable powder, Cinnabaris is added 6 times of weight water grind to pasty state, then added the entry stirring by 1: 65, standing, inclining suspension, and sediment grinds again; As above method is 4 times repeatedly, merges each time suspension, and is standing, gets and is deposited in 35 ℃ of airings, and levigation namely gets the Cinnabaris impalpable powder; The impalpable powder of above-mentioned four kinds of crude drug is mixed to get medicated powder.

5. drug combination preparation as claimed in claim 3 is characterized in that the preparation method of the preparation method Chinese medicine powder of said preparation is:

6. as claim 1,2,5 described drug combination preparations, it is characterized in that the preparation method of intermediate compound I in the preparation method of said preparation comprises the steps:

With the common heating water reflux, extract, of crude drug Radix Scutellariae, Rhizoma Coptidis, Fructus Gardeniae, Radix Curcumae and Gypsum Fibrosum 3 times, each 1.5 hours, merge decocting liquid, be concentrated into relative density 1-1.2, centrifugal, supernatant is crossed macroporous resin column, 50% ethanol elution, reclaim ethanol, concentrated, namely get intermediate compound I; Or after drying the powder of intermediate compound I;

Or: with the first heating water reflux, extract, of Radix Curcumae 3 times, each 1.5 hours, volatile oil was standby; Radix Curcumae medicinal residues and the common decocting of crude drug Radix Scutellariae, Rhizoma Coptidis, Gypsum Fibrosum and Fructus Gardeniae boil 3 times, and each 1.5 hours, merge decocting liquid, be concentrated into thick paste; 50% ethanol that adds 7 times of weight in thick paste, standing 24 hours, get supernatant and reclaim ethanol, concentrated, spray into volatile oil, namely get intermediate compound I; Or after drying the powder of intermediate compound I;

Or: with the first heating water reflux, extract, of Radix Curcumae 3 times, each 1.5 hours, volatile oil was standby; Radix Curcumae medicinal residues and the common decocting of crude drug Radix Scutellariae, Rhizoma Coptidis, Gypsum Fibrosum and Fructus Gardeniae boil 3 times, and each 1.5 hours, merge decocting liquid, be concentrated into relative density 1-1.2, centrifugal, supernatant is crossed macroporous resin column, and 50% ethanol elution reclaims ethanol, concentrated, spray into volatile oil, namely get intermediate compound I; Or after drying the powder of intermediate compound I.

7. drug combination preparation as claimed in claim 3 is characterized in that the preparation method of intermediate compound I in the preparation method of said preparation comprises the steps:

8. drug combination preparation as claimed in claim 4 is characterized in that the preparation method of intermediate compound I in the preparation method of said preparation comprises the steps:

9. as claim 1,2,5,7 or 8 described drug combination preparations, it is characterized in that the crude drug of said preparation consists of:

Borneolum Syntheticum 2 weight portion Pulvis Cornus Bubali Concentratus 13 weight portion Concha Margaritifera 5 weight portions;

Or

Borneolum Syntheticum 1 weight portion Pulvis Cornus Bubali Concentratus 18 weight portion Concha Margaritifera 3 weight portions;

Or

10. drug combination preparation as claimed in claim 3 is characterized in that the crude drug of said preparation consists of:

Or

11. drug combination preparation as claimed in claim 4 is characterized in that the crude drug of said preparation consists of:

Or

12. drug combination preparation as claimed in claim 6 is characterized in that the crude drug of said preparation consists of:

Or

13. drug combination preparation as claimed in claim 1 is characterized in that described capsule is soft capsule, described tablet is dispersible tablet.

14. have application in the medicine of analgesic or hypotensive effect as claim 1,2,5, the arbitrary described drug combination preparation of 7-8,10-12 in preparation.

15. drug combination preparation as claimed in claim 3 has application in the medicine of analgesic or hypotensive effect in preparation.

16. drug combination preparation as claimed in claim 4 has application in the medicine of analgesic or hypotensive effect in preparation.

17. drug combination preparation as claimed in claim 6 has application in the medicine of analgesic or hypotensive effect in preparation.

18. drug combination preparation as claimed in claim 9 has application in the medicine of analgesic or hypotensive effect in preparation.