CN102008890B - Biological control method for biomass excessive accumulation in waste gas biofilter - Google Patents

Biological control method for biomass excessive accumulation in waste gas biofilter Download PDF

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CN102008890B
CN102008890B CN2010105233438A CN201010523343A CN102008890B CN 102008890 B CN102008890 B CN 102008890B CN 2010105233438 A CN2010105233438 A CN 2010105233438A CN 201010523343 A CN201010523343 A CN 201010523343A CN 102008890 B CN102008890 B CN 102008890B
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toluene
exhaust gas
ciliates
nematode
biomass
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CN102008890A (en
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杨春平
金聪颖
杨海宁
罗胜联
何慧军
张晓明
刘勇刚
刘莉华
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南昌航空大学
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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Abstract

一种废气生物过滤器中生物量过度蓄积的生物控制方法,方法步骤为:选定生长正常的线虫和纤毛虫,通过不断地向无机盐液体培养基中通入目标废气,驯化耐受目标废气强捕食生物量大的线虫和纤毛虫;选取甲苯作为目标废气,在甲苯浓度在80~150mg/m3范围内,甲苯对纤毛虫与线虫的影响并不明显,甲苯降解菌的去除率达99-100%;当甲苯浓度升高到600mg/m3后,线虫的生长受到了抑制。 Biological control method of an excessive accumulation of biomass biofilter exhaust gas, the method steps: the selected normal growth and ciliates nematodes, by constantly target exhaust gas into the liquid inorganic medium, the target exhaust resistant domesticated strong high biomass of predator nematodes and ciliate; toluene as the removal rate of selected target exhaust gas, the concentration of toluene in the 80 ~ 150mg / m3 range, ciliates Effect of toluene on nematode is not obvious, toluene degrading bacteria 99- 100%; increased when the concentration of toluene to 600mg / m3, nematode growth is inhibited. 本发明的优点是:本发明可以有效地控制废气生物过滤器中生物量过度蓄积,基本实现生物膜零增长,维持生物过滤器长期稳定运行。 Advantage of the present invention are: the present invention can effectively control exhaust biofilter excessive accumulation of biomass, to achieve substantially zero biofilm growth, to maintain long-term stable operation of the biofilter.

Description

一种废气生物过滤器中生物量过度蓄积的生物控制方法 An exhaust gas biofilter biological control methods excessive accumulation of biomass

技术领域 FIELD

[0001] 本发明涉及一种生物控制方法,尤其涉及一种废气生物过滤器中生物量过度蓄积的生物控制方法。 [0001] The present invention relates to a method for biological control, in particular, it relates to a method for biological control in an exhaust gas biofilters excessive accumulation of biomass.

背景技术 Background technique

[0002] 利用生物方法控制微生物蓄积速率是依据生态学原理,利用微型动物的吞食作用减少气相生物膜的蓄积,即食物链越长,能量损失越大,则产生的生物量也越低。 [0002] The use of biological control of microbial accumulation rate method is based on the ecological principle, by using the swallowing action of animalcules to reduce the accumulation of biofilm gas phase, i.e., the longer the food chain, the greater the energy loss, the biomass produced is also lower. 与研究过的物理控制方法和化学控制方法相比,利用生物方法控制生物膜的过量蓄积具有成本低和操作简单等优点。 Compared to a physical control method and a control method studied chemical, biological method of controlling the use of excess biofilm accumulation of low cost and easy operation. 但是,研究仅仅减缓细菌的蓄积速度,而不能实现细菌的零增长,即不能实现生物过滤反应器的长期稳定运行。 However, the study only slow the rate of accumulation of bacteria, the bacteria can not be achieved zero growth, that can not achieve long-term stable operation of the biofilter reactor. 原生动物作为一类单细胞动物,一般仅能吞食比自身小的微生物(如游离的细菌)或有机颗粒,而气相生物反应器中的细菌和微生物大多以生物膜或絮状污泥等形式存在,这些群聚的微生物特别是生物膜中的微生物可以抵制原生动物的吞食。 As a class of protozoa unicellular animal, generally smaller than themselves only swallowed microorganisms (e.g., bacteria free) or organic particles, and gas phase bioreactor most bacteria and microorganisms in the form of a biofilm or flocculent sludge these microorganisms particular cluster may resist microbial biofilm swallowed protozoa. 同时,还存在原生动物从生物过滤反应器中快速消失的现象,说明通常气相生物过滤反应器的操作条件可能不是这些原生动物的最佳生存条件。 Meanwhile, there is also the filter reactor protozoa rapidly disappearing from a biological phenomenon, the operation of a gas phase conditions typically biofilter reactor may not be optimal living conditions of these protozoa. 利用后生动物(或者后生动物和原生动物混合种群)吞食生物膜来降低生物膜的过度蓄积,就可望解决克服采用采用原生动物时的不足之处。 The use of metazoan (or metazoan and protozoan mixed population) swallowed biofilm to reduce the excessive accumulation of biofilm, it is expected to solve the shortage overcome adopted when using protozoan place. 在对生物过滤器中后生动物的适宜生长条件以及后生动物吞食生物膜的影响机制进行深入研究的基础上,可望实现生物膜的零增长和生物过滤器的长期稳定运行。 Based on the mechanism of biological effects of filters suitable growth conditions metazoan and metazoan swallowed biofilm-depth study on the long-term stable operation is expected to achieve zero growth and biofilm biological filter.

发明内容 SUMMARY

[0003] 本发明的目的在于提供一种废气生物过滤器中生物量过度蓄积的生物控制方法, [0003] The object of the present invention to provide a method of biological control in an exhaust gas biofilters excessive accumulation of biomass,

[0004] 本发明是这样来实现的,其特征是方法步骤为:步骤1.从活性污泥中选定生长正常的线虫和纤毛虫作为高营养级微型动物;步骤2.从生物过滤器中筛选甲苯降解菌;步骤3.通过不断地向加有无机盐液体培养基的锥形瓶中通入目标废气,驯化耐受目标废气强捕食生物量大的线虫和纤毛虫:选取甲苯作为目标废气,在甲苯浓度在8(T150 mg/m3范围内,甲苯对纤毛虫与线虫的影响并不明显,甲苯降解菌的去除率达99-100%;当甲苯浓度升高到600 mg/m3后,线虫的生长受到了抑制,纤毛虫的生长正常,甲苯降解菌的比生长速率下降明显;当甲苯浓度升高至900 mg/m3时,纤毛虫的生长也受到了抑制;步骤4.经过驯化的线虫和纤毛虫扩大培养,将目标废气降解菌接入液体的牛肉膏蛋白胨培养基上,在28°C下180次/分钟摇床振荡培养48小时得到细菌液;然后将细菌液加入到线虫纤 [0004] The present invention is achieved, characterized in that the method steps: Step 1. Select the normal growth as nematodes and ciliates from higher trophic levels Microfauna activated sludge; Step 2. From the biofilter screening toluene degrading bacteria; step 3. by constantly added to the liquid inorganic medium Erlenmeyer flask into target exhaust gas, the exhaust gas domesticated withstand strong prey organisms large target nematode and ciliates: selecting a target exhaust gas toluene in toluene in a concentration of 8 (T150 mg / m3 range, ciliates Effect of toluene on nematode is not obvious, toluene degrading bacteria removal rate is 99-100%; when the concentration of toluene was raised to 600 mg / m3, the nematode growth is inhibited, growth of normal ciliates, toluene degrading bacteria specific growth rate decreased significantly; when the concentration of toluene increased to 900 mg / m3, ciliates growth is also suppressed; step 4. domesticated nematodes and ciliates expansion for the degradation of the target exhaust gas beef extract peptone medium access bacterial liquid cultured at 28 ° C 180 times / min to obtain a bacterial shaker was shaken 48 hours; then, the bacteria were added to the fiber nematode 虫共同培养基,再加入经过驯化消过毒的线虫和纤毛虫,置于恒温恒湿培养箱,在湿度50-60%、温度25°C条件下培养7天,即可得到所需的接种线虫和纤毛虫;步骤5.将培养好的线虫和纤毛虫接种于生物过滤器中,发挥其捕食作用。 Insects common medium, then add sterile domesticated nematodes and ciliates, placed in constant temperature and humidity incubator, and cultured at 50-60% humidity, at a temperature of 25 ° C, 7 days, to give the desired inoculum nematodes and ciliates; step 5. culturing ciliates and good nematode inoculated in a biological filter, which play a role in prey.

[0005] 所述的目标废气降解菌为从废气生物过滤器中筛选所得,用无机盐培养基浸泡废气生物过滤反应器内填料,5 min后接取微生物悬浮液I 1,取100 ml悬浮液到250 ml锥形瓶中,加入目标废气甲苯驯化,取样稀释10_4,10_5,10_6,在常用牛肉膏蛋白胨培养基上涂平板,28°C培养2 d,所得菌落形态为光滑平坦,规则圆形,半透明,直径4 _左右,革兰式阳性菌,短杆状;甲苯净化情况:在甲苯气相浓度为3213 mg/m3的条件下摇床培养18、42 h去除效率分别为89.7,99.3%。 [0005] The goal of the exhaust gas from the exhaust gas to screen degrading bacteria biofilter resulting immersed biofilter reactor exhaust gas with inorganic filler medium, 5 min after the microbial suspension acess I 1, taking 100 ml suspension to 250 ml Erlenmeyer flask was added toluene domesticated target exhaust gas, the sample was diluted 10_4,10_5,10_6, were plated on conventional beef extract peptone medium, 28 ° C culture 2 d, the resulting colony morphology is smooth and flat, circular rules , translucent, _ a diameter of about 4, formula Gram-positive bacteria, short rod; toluene purification: the concentrations of toluene vapor removal efficiency shaker 18,42 h under 3213 mg / m3, respectively of 89.7,99.3% .

[0006] 所述的无机盐培养基配方为氯化铵1.0 g,磷酸二氢钾2.0 g,磷酸氢二钾3.0 g,硫酸镁0.7 g,氯化钙0.02 mg,硫酸亚铁0.5 mg, I ml的微量金属痕量溶液,微量金属痕量溶液由硫酸铜5.0 mg,氯化猛5.0 mg和硫酸锌10.0 mg组成,加水定容至11,高压灭菌。 [0006] The inorganic salt medium were ammonium chloride 1.0 g, potassium dihydrogen phosphate 2.0 g, dipotassium phosphate 3.0 g, magnesium sulfate 0.7 g, calcium chloride 0.02 mg, ferrous sulfate 0.5 mg, I trace ml of trace metals solution, trace metals solution was composed trace sulfate 5.0 mg, 5.0 mg and Meng chloride 10.0 mg consisting of zinc sulfate, 11 to add water, autoclaving.

[0007] 本发明的优点是:本发明可以有效地控制废气生物过滤器中生物量过度蓄积,基本实现生物膜零增长,维持生物过滤器长期稳定运行。 Advantages [0007] of the present invention are: the present invention can effectively control exhaust biofilter excessive accumulation of biomass, to achieve substantially zero biofilm growth, to maintain long-term stable operation of the biofilter.

附图说明 BRIEF DESCRIPTION

[0008] 图1是甲苯浓度为150 mg toluene m_3,细菌、纤毛虫和线虫的数量图; [0008] FIG. 1 is a toluene concentration of 150 mg toluene m_3, bacteria, and nematodes Number FIG ciliates;

[0009] 图2是甲苯浓度为600 mg toluene m_3,细菌、纤毛虫和线虫的数量图; [0009] FIG. 2 is a toluene concentration of 600 mg toluene m_3, bacteria, and nematodes Number FIG ciliates;

[0010] 图3是甲苯浓度为900 mg toluene m_3,细菌、纤毛虫和线虫的数量图。 [0010] FIG. 3 is a toluene concentration of 900 mg toluene m_3, bacteria, ciliates, and the number of nematodes in FIG.

[0011] 图例说明图1、2和3中(.)为对照组细菌,(■)为实验组细菌,(▲)为纤毛虫,(X)为线虫。 [0011] Legend 1, 2 and 3 (.) Is the group of bacteria, (■) for the experimental group of bacteria, (▲) as ciliates, (X) is a nematode.

具体实施方式 Detailed ways

[0012] 以甲苯为目标废气实验,步骤如下: [0012] In toluene as a target exhaust gas test, the following steps:

[0013] I从废气生物过滤器内筛选降解甲苯的菌株 [0013] I Toluene Degradation screening strain from the exhaust gas biofilter

[0014] 用无机盐培养基浸泡生物过滤反应器内填料,5 min后接取微生物悬浮液11。 [0014] immersed biofilter reactor with inorganic filler medium, then 5 min after taking 11 microbial suspension. 取100 ml悬浮液到250 ml锥形瓶,放在摇床上振荡30 min,使菌体、芽孢或孢子均勻分散,制成10_2稀释度的稀释液。 100 ml suspension was taken into 250 ml Erlenmeyer flask, shaken in a shaker for 30 min, so that cells, spores or spores uniformly dispersed to prepare a diluted solution 10_2 dilution. 然后按10倍稀释法进行稀释分离,制成10_3、10_4、10_5、10_6的稀释液。 Then press diluted 10-fold dilution was separated, made of 10_3,10_4,10_5,10_6 dilution. 采用梯度平板法进行分离:在灭菌培养皿中,先倾倒10 ml牛肉膏蛋白胨固体培养基,将培养皿一侧放置木条上,使皿中的培养基倾斜成斜面,而且刚好完全盖住培养皿底部,待培养基凝固后,将培养皿放平,再倾倒10 ml已融化的含30 ??1甲苯的牛肉膏蛋白胨固体培养基,刚好完全盖住下层斜面,由于甲苯的扩散作用,上层培养基薄的部分甲苯浓度大大降低,造成上层培养基由厚到薄甲苯浓度递减的梯度。 Separated by gradient plate method: In sterilized Petri dish, was poured to 10 ml of beef extract peptone solid medium, placed on the wood side dish, culture medium in the dish inclined ramp, and just completely cover the bottom of the dish until the medium solidified, the plates were flat, then was poured 10 ml of beef extract peptone solid medium containing 30 ?? 1 toluene melted, just completely cover the lower inclined surface, since the diffusion of toluene, concentration of toluene supernatant medium thin portion is greatly reduced, resulting in supernatant medium from thick to thin down the concentration gradient of the toluene. 梯度平板冷凝后,用无菌移液管分另帳取10_6、10_5、10_4三个稀释度的菌悬液0.1 ml,依次滴加于相应编号已制备好的梯度平板上,用无菌玻璃凃棒将菌液自平板中央向四周涂布扩散,28 °C培养2 d,将在甲苯高浓度区出现的菌落依次在含甲苯浓度递增的牛肉膏蛋白胨固体培养基平板上连续划线分离,最后挑取单菌落接种到含甲苯的牛肉膏蛋白胨固体斜面培养基上,28 °C培养2 d。 After condensation gradient plate, using a sterile pipette to take account of another sub-three bacterial suspension and dilutions 10_6,10_5,10_4 0.1 ml, were added dropwise over the corresponding number of the prepared gradient plates in a good coated sterile glass the bacteria from the central flat bar coating to spread around, 28 ° C culture 2 d, colonies appeared in the high concentration region of toluene with continuous streaked sequentially increasing concentrations of a toluene peptone beef extract solid media plates, and finally Individual colonies were picked beef extract was inoculated into a slant medium containing peptone solid toluene, 28 ° C culture 2 d. 所得菌落形态为光滑平坦,规则圆形,半透明,直径4 _左右,革兰式阳性菌,短杆状。 The resultant colony morphology is smooth and flat, round about 4 _ rules, translucent, diameter, type of Gram-positive bacteria, short rod. 甲苯净化情况:在甲苯气相浓度为3213 mg m_3的条件下摇床培养18、42 h去除效率分别为89.7,99.3%。 Toluene Purification: The concentrations of toluene vapor under conditions of 3213 mg m_3 shaker 18,42 h 89.7,99.3 percent removal efficiency.

[0015] 2从活性污泥中选定微型动物 [0015] 2 is selected from activated sludge Microfauna

[0016] 从长沙市第二污水处理厂取活性污泥,接种10%至250 ml锥形瓶中,其中有100ml无机盐培养基,分阶段滴加2,4,6,8 μ I甲苯,在显微镜下观察其中微生物的组成,发现线虫和纤毛虫可以正常生长,从而选定这两种微型生物作为引入种群。 [0016] Changsha taken from the second activated sludge sewage treatment plant, 10% of inoculated to 250 ml Erlenmeyer flask, 100ml of which inorganic salt medium, a phased 2,4,6,8 μ I of toluene was added dropwise, observed under a microscope in which the composition of microorganisms, nematodes and found ciliates can grow normally, thereby selecting the two populations is introduced as micro-organisms. 无机盐培养基组成每升包括:氯化铵1.0 g,磷酸二氢钾2.0 g,磷酸氢二钾3.0 g,硫酸镁0.7 g,氯化钙0.02mg,硫酸亚铁0.5 mg, I ml的微量金属痕量溶液(硫酸铜5.0 mg,氯化猛5.0 mg,硫酸锌10.0mg)。 Per liter of inorganic salt medium composition comprising: chloride 1.0 g, potassium dihydrogen phosphate 2.0 g, dipotassium phosphate 3.0 g, magnesium sulfate 0.7 g, calcium chloride 0.02mg, ferrous sulfate 0.5 mg, I ml of trace trace metal solution (copper sulfate 5.0 mg, Meng chloride 5.0 mg, zinc sulfate 10.0mg).

[0017] 3动态驯化选定线虫和纤毛虫 [0017] 3 and nematodes ciliates selected dynamic acclimation

[0018] 采用5个不同甲苯气相浓度,80,150,300,600,900 mg πΓ3。 [0018] The five different concentrations of toluene vapor, 80,150,300,600,900 mg πΓ3. II锥形瓶中加入500 ml培养基,接种IO7 πιΓ1甲苯降解菌和I ml Γ1线虫和纤毛虫混合液,运行200 h。 II Erlenmeyer flask was added 500 ml medium was inoculated IO7 πιΓ1 toluene degrading bacteria and nematodes, and I ml Γ1 mixture ciliates, operation 200 h. 实验所用锥形瓶用橡胶瓶塞封紧,含甲苯的空气是通过转子流量计控制平均流量为120 I通过管子延伸到瓶的底部。 Experiments tamping with a rubber stopper conical flask, air containing toluene was controlled by a rotameter average flow of 120 I at the bottom by a tube extending to the bottle. 空气通过空气压缩机,被划分成两个支流。 Through the air compressor is divided into two substreams. 一支是通过加湿瓶,而另一支通过液体甲苯试剂瓶(98.5%,分析试剂级,祥和化工厂,湖南,中国)。 Through a humidifier bottle, and the other branch of toluene by a liquid reagent bottle (98.5% analytical reagent grade, chemical peaceful, Hunan, China). 这两支气流分别由转子流量计(LZB - 10,余姚市银环流量计有限公司,江苏省,中国)控制进入混合气瓶混合,取得所需甲苯气相浓度。 Respectively, by these two gas flow rotameter (LZB - 10, Yuyao Yinhuan meter Co., Jiangsu Province, China) control proceeds mixing mixing cylinder, to obtain the desired concentrations of toluene vapor. 结果甲苯浓度范围在80-150 mg toluene m_3,细菌,线虫和纤毛虫生长均不受影响,细菌去除率接近100% (如图1所示);甲苯浓度范围在150-600mg toluene m_3,细菌和纤毛虫的生长没有受到影响,但是抑制了线虫的生长(如图2所示);甲苯浓度范围在600-900 mg toluene πΓ3,纤毛虫的生长慢慢受到抑制,在900 mg toluenem_3,纤毛虫生长受到抑制(如图3所示)。 Results toluene in a concentration range of 80-150 mg toluene m_3, bacteria, nematodes, and growth will not be affected ciliates, bacteria removal efficiency close to 100% (Figure 1); toluene concentration range 150-600mg toluene m_3, bacteria and ciliates growth was not affected, but inhibit the growth of the nematode (FIG. 2); toluene concentration in the range 600-900 mg toluene πΓ3, inhibited the growth of ciliates slowly, in 900 mg toluenem_3, ciliates growth suppressed (Figure 3).

[0019] 4线虫和纤毛虫的扩大培养 [0019] 4 expanded culture of nematodes and ciliates

[0020] 将甲苯降解菌接入液体的牛肉膏蛋白胨培养基上,在28°C下180次/min摇床振荡培养48 h得到细菌液;然后将细菌液加入到微型动物培养基,再加入经过驯化消过毒的线虫和纤毛虫,置于恒温恒湿培养箱,在湿度50-60%、温度25%条件下培养7 d,即可得到所需的接种线虫和纤毛虫。 [0020] The upper beef extract peptone medium access toluene degrading bacteria liquid at 28 ° C 180 times / min to obtain a shaker bacterial culture was 48 h; then was added to the bacterial culture medium animalcules, then add domesticated sterilized nematodes and ciliates, placed in constant temperature and humidity incubator, culture 7 d humidity of 50-60%, at a temperature condition of 25%, to obtain the desired inoculum nematodes and ciliates. 线虫纤毛虫共同培养基配方为用975 ml蒸馏水溶解3 g氯化钠,8g琼脂粉,2.5 g蛋白胨,高压蒸汽灭菌。 Nematodes ciliates together with 975 ml medium were dissolved in distilled water 3 g NaCl, 8g agar powder, 2.5 g peptone, autoclaving. 待冷却至55°C左右后,加入I ml I mol/1 CaCl2, After cooling to about 55 ° C, was added I ml I mol / 1 CaCl2,

I ml I mol/1 MgSO4, I ml 5 mg/1 胆固醇,25 ml 憐酸钾缓冲液。 I ml I mol / 1 MgSO4, I ml 5 mg / 1 cholesterol, 25 ml of potassium pity buffer. I mol/1 CaCl2:110.99g CaCl2,加水定容至II,高压灭菌。 I mol / 1 CaCl2: 110.99g CaCl2, add water to II, autoclaving. I mol/1 MgSO4:246.47 g MgSO4.7H20,加水定容至I1,高压灭菌;I mol/1磷酸钾缓冲液:108.3 g KH2PO4,35.6 g K2HPO4,加水定容至II ;5 mg/ml胆固醇:5 g胆固醇,加无水乙醇定容至II。 I mol / 1 MgSO4: 246.47 g MgSO4.7H20, add water to I1, autoclaved; I mol / 1 potassium phosphate buffer: 108.3 g KH2PO4,35.6 g K2HPO4, add water to II; 5 mg / ml cholesterol : 5 g cholesterol, and absolute ethanol to II.

Claims (3)

1.一种废气生物过滤器中生物量过度蓄积的生物控制方法,其特征是方法步骤为:步骤1.从活性污泥中选定生长正常的线虫和纤毛虫作为高营养级微型动物;步骤2.从生物过滤器中筛选甲苯降解菌;步骤3.通过不断地向加有无机盐液体培养基的锥形瓶中通入目标废气,驯化耐受目标废气强捕食生物量大的线虫和纤毛虫:选取甲苯作为目标废气,在甲苯浓度在8(Tl50mg/m3范围内,甲苯对纤毛虫与线虫的影响并不明显,甲苯降解菌的去除率达99-100% ;当甲苯浓度升高到600 mg/m3后,线虫的生长受到了抑制,纤毛虫的生长正常,甲苯降解菌的比生长速率下降明显;当甲苯浓度升高至900 mg/m3时,纤毛虫的生长也受到了抑制;步骤4.经过驯化的线虫和纤毛虫扩大培养,将目标废气降解菌接入到液体的牛肉膏蛋白胨培养基上,在28°C下180次/分钟摇床振荡培养48小时得到细菌液 CLAIMS 1. A method for biological control in an exhaust gas biofilters excessive accumulation of biomass, which method is characterized in the following steps: Step 1. Select the normal growth as nematodes and ciliates higher trophic levels miniature animals from activated sludge; Step 2. screening from toluene degrading bacteria biofilter; step 3. by constantly added to the liquid inorganic medium Erlenmeyer flask into target exhaust gas, the exhaust gas domesticated withstand strong prey organisms large target nematode and fiber caterpillars: select toluene as target exhaust gas, the concentration of toluene in the 8 (Tl50mg / m3 range, ciliates Effect of toluene on nematode is not obvious, toluene degrading bacteria removal rate is 99-100%; when the concentration of toluene was raised to after the 600 mg / m3, nematode growth is inhibited, growth of normal ciliates, toluene degrading bacteria specific growth rate decreased significantly; when the concentration of toluene increased to 900 mg / m3, ciliates growth is also suppressed; step 4. expansion for domesticated ciliates and nematodes, the target exhaust gas access to the degrading bacterium peptone beef extract liquid at 28 ° C 180 times / minute for 48 hours to give a shaker bacteria solution ;然后将细菌液加入到线虫纤毛虫共同培养基,再加入经过驯化消过毒的线虫和纤毛虫,置于恒温湿培养箱,在湿度50-60%、温度25°C条件下培养7天,即可得到所需的接种线虫和纤毛虫;步骤5.将培养好的线虫和纤毛虫接种于生物过滤器中,发挥其捕食作用。 ; The bacteria were then added to the liquid medium nematode common ciliate, domesticated added nematodes and sterile ciliates, placed in a thermostatic moist incubator, and cultured at 50-60% humidity, at a temperature of 25 ° C, 7 days , to give the desired inoculum and nematode ciliate; step 5. culturing ciliates and good nematode inoculated in a biological filter, which play a role in prey.
2.根据权利要求1所述的一种废气生物过滤器中生物量过度蓄积的生物控制方法,其特征在于:所述的目标废气降解菌为从废气生物过滤器中筛选所得,用无机盐培养基浸泡废气生物过滤反应器内填料,5min后接取微生物悬浮液1L,取IOOmL悬浮液到250mL锥形瓶中,加入目标废气甲苯驯化,取样稀释10_4、10_5、10_6,在常用牛肉膏蛋白胨培养基上涂平板,28°C培养2d,所得菌落形态为光滑平坦,规则圆形,半透明,直径4mm左右,革兰氏阳性菌,短杆状;甲苯净化情况:在甲苯气相浓度为3213 mg/m3的条件下摇床培养18、42h去除效率分别为89.7,99.3%ο The method of biological control organisms An exhaust gas filter of claim 1 in excessive accumulation of biomass as claimed in claim wherein: said target exhaust gas resulting from degrading bacteria to screen a biological filter an exhaust gas, with an inorganic salt culture yl soaking exhaust biofilter reactor packing, acess microbial suspension 1L after 5min, the suspension was taken IOOmL 250mL conical flask, toluene domesticated target exhaust gas, the sample was diluted 10_4,10_5,10_6, in a conventional beef extract peptone coated plates, 28 ° C on a culture substrate 2d, the resulting colony morphology is smooth and flat, approximately circular rule, translucent, diameter 4mm, gram-positive bacteria, short rod; toluene purification: the toluene vapor concentration 3213 mg shaking culture under the conditions of removal efficiency 18,42h / m3 respectively for 89.7,99.3% ο
3.根据权利要求2所述的一种废气生物过滤器中生物量过度蓄积的生物控制方法,其特征在于:所述的无机盐培养基配方为1.0g氯化铵、2.0g磷酸二氢钾、3.0g磷酸氢二钾、`0.7g硫酸镁、0.02mg氯化韩、0.5mg硫酸亚铁、ImL微量金属痕量溶液,该配方加水溶解并定容至1L,高压灭菌,其中,微量金属痕量溶液配制如下:称取硫酸铜0.5g、氯化锰0.5g、硫酸锌1.0g,加水溶解并定容至100mL。 The method of biological control organisms An exhaust gas filter of claim 2 in excessive accumulation of biomass as claimed in claim wherein: the inorganic salt medium were 1.0g of ammonium chloride, 2.0 g of potassium dihydrogen phosphate , 3.0 g of dipotassium hydrogen phosphate, `0.7g of magnesium sulfate, chloride Han 0.02mg, 0.5mg ferrous sulfate, ImL trace trace metals solution, the formulation dissolved in water and dilute to 1L, autoclaving, wherein trace trace metals solution was prepared as follows: Weigh sulfate 0.5g, manganese chloride 0.5g, zinc sulfate 1.0g, dissolved in water and dilute to 100mL.
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