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CN101975769A - Human tissue autofluorescence detection system based on excitation of light sources with different wavelength - Google Patents

Human tissue autofluorescence detection system based on excitation of light sources with different wavelength Download PDF

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CN101975769A
CN101975769A CN 201010284908 CN201010284908A CN101975769A CN 101975769 A CN101975769 A CN 101975769A CN 201010284908 CN201010284908 CN 201010284908 CN 201010284908 A CN201010284908 A CN 201010284908A CN 101975769 A CN101975769 A CN 101975769A
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detection
tissue
human
part
system
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CN 201010284908
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Chinese (zh)
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刘丽娜
李伟华
李步洪
聂英斌
谢树森
陈德福
黄志勇
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福建师范大学
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Abstract

The invention relates to an autofluorescence detection system for exciting specific endogenous fluorescent substances in a human tissue by light sources with different wavelengths and detecting information of fluorescence with specific wavelength corresponding to the fluorescent substances in endoscopy for the human early tumor tissue. The autofluorescence detection system mainly consists of an excitation light source part, an excitation-detection-acquisition part, an optical collection part, a signal preprocessing part and a computer part. The autofluorescence detection system is characterized in that a tunable laser (1) can adjust the needed pulse laser wavelengths according to the specific endogenous fluorescent substances in the human tissue, and a filter wheel (10) is equipped with narrow band pass filters with different center wavelengths. The system can excite the specific endogenous fluorescent substances in the human tissue by a plurality of optimal excitation wavelengths sequentially, thus avoiding the problem of no benefit for analysis owing to insufficient fluorescence information or information overlap and the like caused by excitation of single wavelength or broad spectrum light sources, and improving sensitivity and specificity of early tumor detection.

Description

一种基于不同波长光源激发的人体组织自体荧光检测系统 Based on human tissue excitation light sources of different wavelengths of autofluorescence detection system

技术领域 FIELD

[0001] 本发明属生物医学类仪器,涉及一种人体组织自体荧光检测系统,尤其是一种可用于人体早期肿瘤组织的内窥镜检查,利用不同波长光源激发人体组织中特定的内源性荧光物质,并检测与这些荧光物质相对应的特定波长荧光信息的自体荧光检测系统。 [0001] The present invention belongs to biological medical instrument, to a body tissue autofluorescence detection system, in particular for an early stage tumor endoscopic body, excitation light sources with different wavelengths human tissue specific endogenous a fluorescent substance, the fluorescent substance and detecting autofluorescence corresponding to a specific wavelength of the fluorescence detecting system information.

技术背景 technical background

[0002] 现有的人体组织自体荧光检测系统是通过利用普通内窥镜,并采用单一波长或宽光谱的光源激发人体组织的内源性荧光物质,利用正常和肿瘤组织的自体荧光光谱差异对人体组织进行荧光检测的系统。 [0002] Existing human tissue autofluorescence detection system through the use of an ordinary endoscope, and a single wavelength excitation source or a broad spectrum fluorescent substance endogenous human tissues, normal and tumor tissue using autofluorescence spectra differences human tissue fluorescence detection system. 人体组织的自体荧光主要来源于氨基酸、结构蛋白、 酶、辅酶、脂肪、维生素和卟啉等物质,它们各自具有不同的最佳荧光激发波长和与之对应的荧光发射波长,其中常见的内源性荧光物质如色氨酸、胶原蛋白、还原型烟酰胺腺嘌呤二核苷酸、吓啉和黄素腺嘌呤二核苷酸等所对应的最佳激发波长分别为280、325、340、400 和450nm,相应的最佳荧光发射波长分别为335、390、460、635和535nm。 Human tissue autofluorescence mainly from amino acids, structural proteins, enzymes, coenzymes, fat, vitamins and other porphyrin substances, each having different optimal fluorescence excitation wavelength and fluorescence emission wavelength corresponding thereto, wherein the common source fluorescent substance such as tryptophan, collagen, reduced nicotinamide adenine dinucleotide, morpholine and the optimal excitation wavelength scare flavin adenine dinucleotide, etc. corresponding respectively 280,325,340,400 and 450nm, corresponding optimal fluorescence emission wavelength, respectively 335,390,460,635 and 535nm. 在某一特定波长激发光源的激发下,人体组织自体荧光光谱是多种内源性荧光物质光谱的叠加。 At an excitation wavelength of the excitation light source of a specific human tissue autofluorescence spectra of the various substances is superimposed on the intrinsic fluorescence spectrum. 截至目前,已获得临床初步应用的自体荧光检测系统有(I)Xillix-LIFE lung/GI/ENT(Xillix Technologies Corp, Richmond, BC, Canada) ( WWik^ 442nm) ; (2) D-Iight System (Karl Storz, Tuttlingen, Germany) ( MR 'iA 442nm) ; (3)WavSTAT, Optical Biopsy System(SpectraScience, San Diego, USA)( # ^ ^ ^ 410nm) ; (4)SAFE-3000(Pentax, Tokyo, Japan) ( ■发M 长408nm) ; (5) Evis LuceraSpectrum(Olympus, Japan) Up to now, has been auto-fluorescence detection system preliminary clinical application are (I) Xillix-LIFE lung / GI / ENT (Xillix Technologies Corp, Richmond, BC, Canada) (WWik ^ 442nm); (2) D-Iight System ( Karl Storz, Tuttlingen, Germany) (MR 'iA 442nm); (3) WavSTAT, Optical Biopsy System (SpectraScience, San Diego, USA) (# ^ ^ ^ 410nm); (4) SAFE-3000 (Pentax, Tokyo, Japan ) (M ■ hair length 408nm); (5) Evis LuceraSpectrum (Olympus, Japan)

长395-475nm)。 Long 395-475nm). 这些自体荧光内窥镜检测系统采用可见光波段的单一波长(408、410和442nm)或宽光谱激发光源(395-475nm),这些激发波长不仅不能激发色氨酸、胶原蛋白和还原型烟酰胺腺嘌呤二核苷酸的荧光,而且不能有效地激发卟啉的荧光。 The autofluorescence endoscopy system using a single wavelength band of visible light (408, 410 and 442 nm) or a broad spectrum excitation source (395-475nm), excitation wavelength which can excite only tryptophan, collagen and glandular reduced nicotinamide purine dinucleotide fluorescence, and can not efficiently excite the fluorescent porphyrin. 因此,系统检测到的荧光信号主要来源于部分卟啉和黄素腺嘌呤二核苷酸。 Thus, the system detects the fluorescent signal from the major part of the porphyrin and flavin adenine dinucleotide. 激发波长过于单一或者过于宽广均未能有效地激发具有诊断意义的荧光信息,在临床应用中尚无法实现人体早期肿瘤组织的高灵敏度和特异性检测。 Excitation wavelength is too simple or too broad failed to effectively excite the fluorescence of information with diagnostic significance in clinical applications still can not achieve high sensitivity and specificity to detect early tumor tissue of the human body.

发明内容 SUMMARY

[0003] 针对现有的人体组织自体荧光检测系统采用单一波长或者宽光谱光源作为激发光源,未能有效地激发具有诊断意义的内源性荧光物质的荧光信息,在临床应用中存在灵敏度和特异性不高等不足,本发明提供一种基于多波长光源激发的人体组织自体荧光检测系统,该系统可采用多个最佳激发波长激发人体组织中特定的内源性荧光物质,检测相应的荧光信息,避免采用单一波长或宽光谱光源激发导致荧光信息不够丰富或信息重叠不利于分析等问题,从而提高人体早期肿瘤组织的检测灵敏度和特异性。 [0003] The single wavelength or a broad spectrum light source for existing human tissue autofluorescence detection system as the excitation source, not effectively excite the fluorescent substance information intrinsic fluorescence of diagnostic interest, the presence of sensitivity and specificity in clinical applications of not higher insufficient, the present invention provides a multi-wavelength light source based on human tissue of excitation autologous fluorescence detection system, the system may employ a plurality of optimal excitation wavelength excitation human tissue specific endogenous fluorescent substance, detection information corresponding fluorescence , to avoid a single wavelength or a broad spectrum light source cause fluorescence excitation information superimposing information is not rich or detrimental problem analysis, thereby improving the detection sensitivity and specificity of early human tumor tissue.

[0004] 为实现本发明的目的采用的技术方案是:自体荧光检测系统由激发光源部分、激发-检测-采集部分、光学收集部分、信号预处理部分和计算机部分构成,其中信号预处理部分与计算机部分通过信号线进行连接,激发光源部分通过激发光与激发-检测-采集部分中的激发光输入端的光纤相连,激发-检测-采集部分中的荧光输出端的光纤通过荧光信号通路与光学收集部分相连,荧光信号通路经过光学收集部分传输后进入信号预处理部分,信号预处理部分中光电倍增管光电转化后变为电信号,进入计算机部分。 [0004] To achieve the object of the technical solution employed in the present invention are: fluorescent detection system by the excitation light source part, self excitation - detection - acquisition part, an optical collection portion, and a computer signal preprocessing section parts, wherein the signal preprocessing portion computer portion are connected by a signal line, the excitation light source section through the excitation light and excitation - detection - excitation light input acquisition part of the optical fiber is connected, excitation - detection - fluorescence output terminal collection portion of the optical fiber by the fluorescent signal path and an optical collection portion connected to a fluorescence signal after passage into the collecting portion of the optical transmission signal preprocessing, signal preprocessing section photomultiplier tube after the photoelectric conversion into electrical signals, into the computer portion.

[0005] 所述的激发光源部分由波长可调谐激光器、光衰减器和耦合透镜组成,构成激发光通路。 Said excitation light source part [0005] of the wavelength tunable laser, optical attenuator and the coupling lenses, constituting the excitation light path. 所述的可调谐激光器可以根据人体组织中特定的内源性荧光物质调节所需的脉冲激光波长,可依次调节的波长范围从210nm到2200nm,克服了现有的人体组织自体荧光检测系统采用单一波长或者宽光谱光源作为激发光源,未能有效地激发具有诊断意义的内源性荧光物质的荧光信息,在临床应用中存在灵敏度和特异性不高等不足;可调谐激光器输出的脉冲激光功率可以通过光衰减器进行调节,调节后的激发光经过耦合透镜耦合进入Y 型光纤的激发光输入端的光纤内。 The tunable laser may be adjusted according to the desired pulse laser wavelength human tissue specific endogenous fluorescent substance, can be successively adjusted wavelength range from 210nm to 2200nm, to overcome the existing human tissue autofluorescence detection system uses a single wavelength or a broad spectrum light source as the excitation source, not effectively excite the fluorescent substance information intrinsic fluorescence of a diagnosis, there is insufficient sensitivity and specificity in clinical applications of higher; tunable laser output from the pulse laser power by optical attenuator is adjusted, the adjusted excitation light is coupled into the coupling lens after the excitation optical input Y-type optical fiber.

[0006] 所述的激发_检测_采集部分由两路呈Y型的光纤和内窥镜组成,其中Y型光纤的一路由六根传输激发光的光纤,另一路为单根传输荧光光纤的荧光输出端。 Detecting excitation _ _ collecting section [0006] to form the Y-shaped fiber and a two-way endoscope, where one Y-type optical fiber is excited by the six transmitting light, the other transmission path is a single fluorescent fiber fluorescence an output terminal. 传输激发光的光纤一端为激发光输入端,另一端为接入端,通过内窥镜的活检通道接入内窥镜内,激发光源部分发出的激发光通过该光纤照射待测组织;传输荧光的光纤一端为荧光输出端,另一端亦为接入端,通过内窥镜的活检通道接入内窥镜内。 A transmission excitation light excitation light input end of the fiber end and the other end of the access terminal, the access through the biopsy channel of an endoscope of the endoscope, the excitation light emitted from excitation light source portion of the optical fiber by the illuminated tissue under test; transmission fluorescence one end of the fluorescent optical fiber output end and the other end is also access, through the biopsy channel of an endoscope access endoscope. 传输激发光的光纤接入端和传输荧光光纤的接入端合并构成Y字型的尾部,传输激发光的光纤输入端和传输荧光光纤的输出端分开构成Y字型的两个头部。 Fiber access terminal and the access terminal transmitting a transmission optical fluorescence excitation light combined tail Y-shaped configuration, the excitation light transmitting optical fiber input end and output end of the optical fiber transmission fluorescence separating two Y-shaped head configuration. 待测组织发出的自体荧光通过该光纤的荧光输出端向光学收集部分传送荧光。 Measured tissue autofluorescence emitted fluorescence transmitted through the fluorescence to the optical output terminal collection portion of the optical fiber. 这样构成“激发光-光纤-内窥镜-照射-激发荧光”和“收集荧光-光纤-传出荧光”光通路。 Such configuration "excitation light - optical fiber - the endoscope - irradiated - excite the fluorescent" and "fluorescence is collected - fiber - Outgoing fluorescence" light path.

[0007] 所述的光学收集部分由滤光轮和两个耦合透镜组成,传输荧光的光纤荧光输出端输出的荧光,通过前耦合透镜耦合后经过滤光轮中与激发光波长相对应的一个滤光片,再由后耦合透镜耦合输入信号预处理部分。 [0007] The optical collection portion of the filter wheel and two coupling lenses, optical fiber output end of the transmission fluorescence fluorescence output, coupled through the coupling lens through the filter wheel in front of a filter corresponding to the excitation light looks rays, and then coupled by the coupling lens after the input signal preprocessing part. 滤光轮采用市场上可购买的通用型多孔滤光轮, 孔中根据对应的波长安装有不同中心波长的窄带滤光片。 Using a common type of porous filter wheel of the filter wheel can be purchased on the market, is mounted in the hole narrowband filter in accordance with different center wavelengths corresponding to the wavelength. 当滤光轮上安装6个窄带滤光片时,中心波长可分别选择335nm,390nm,460nm,520nm,535nm,和635nm,带宽均为20nm,用于分别提取280nm激发光激发产生335nm的荧光信号,340nm激发光激发产生390和460nm的荧光信号,460nm激发光激发产生520nm的荧光信号,450nm激发光激发产生535nm的荧光信号和400nm激发光激发产生635nm的荧光信号。 When the narrowband filter installation 6 on the filter wheel, can be selected for the center wavelength of 335nm, 390nm, 460nm, 520nm, 535nm, and 635nm, bandwidth of 20 nm are, for extracting 335nm 280nm fluorescence excitation light signals respectively generated , 340nm excitation light 390 and the fluorescent signal of 460nm, 460nm excitation light of the fluorescent signal 520nm, 450nm excitation light and the fluorescent signal of 535nm 400nm excitation light of 635nm fluorescent signal. 其中,波长可调谐激光器和滤光轮由计算机部分同步控制,实现激发光波长与滤光轮上的滤光片的一一对应。 Wherein the wavelength tunable laser and computer controlled filter wheel synchronization section, realized on the filter correspond excitation light wavelength and the filter wheel.

[0008] 所述的信号预处理部分由光电倍增管、前置放大器、光子计数器和计数板卡组成, 进入光电倍增管的光信号经过光电转化后变为电信号,依次经过前置放大器、光子计数器和计数板卡,进入计算机部分进行数据采集和处理,构成信号预处理部分。 [0008] The signal preprocessing section photomultiplier tube by a photo, a preamplifier, a photon counter counts and composition board, the optical signal enters a photomultiplier tube after the photoelectric conversion into electrical signals, sequentially passes through a preamplifier, the photon counter and counts the board into the computer data acquisition and processing part, constitute a signal preprocessing part.

[0009] 本发明专利的有益效果是,采用波长可调谐的激光器作为激发光源,获取针对特定内源性荧光物质的最佳激发波长,同时检测相对应的发射荧光,实现了荧光信息的最佳激发和检测;同时,采用滤光片从连续荧光光谱中分解获取分立的荧光波长,这在硬件上实现从连续光谱分解出能够明确表征组织荧光特性的荧光信号,简化数据处理的过程,实现人体组织自体荧光的高灵敏度和特异性检测。 [0009] Advantageous effects of the present invention patent, a wavelength tunable laser as excitation light source, obtain optimal excitation wavelength for a specific endogenous fluorescent substance, while fluorescent emission is detected corresponding to achieve the best fluorescent information excitation and detection; simultaneously using the decomposition filter process from the continuous discrete fluorescence spectra acquired fluorescence wavelength, it is possible to achieve the decomposition of fluorescence signal clearly characterizing the tissue from the fluorescent properties of the continuous spectrum in the hardware, to simplify the data processing, realize the human body tissue specificity and high sensitivity detection of autofluorescence.

附图说明 BRIEF DESCRIPTION

[0010] 图1是本发明系统结构示意图。 [0010] FIG. 1 is a schematic system configuration of the present invention. [0011] 图1中,1为可调谐激光器,2为光衰减器,3为耦合透镜,4为传输激发光的光纤一端的激发光输入端,5为传输激发光光纤接入内窥镜的另一端,6为内窥镜,7为待测组织,8 为传输荧光的荧光输出端,9为前耦合透镜,10为滤光轮,11为后耦合透镜,12为冷却电压控制器,13为冷却器,14为光电倍增管,15为前置放大器,16为单光子计数器,17为计数板卡,18为计算机。 In [0011] FIG. 1, 1 is a tunable laser, an optical attenuator 2, 3 of the coupling lens, 4 the excitation end of the fiber transmission input excitation light, the excitation light optical fiber 5 to transmit access endoscope the other end of the endoscope 6, 7 for the tissue tested, fluorescence output terminal 8 is transmitted fluorescence, 9 is a front coupling lens, filter wheel 10, the coupling lens 11, the voltage controller 12 is cooled, 13 chiller, a photomultiplier tube 14, preamplifier 15, 16 is a single-photon counter, counting board 17, the computer 18.

具体实施方式 detailed description

[0012] 以下结合附图对发明作进一步的说明。 [0012] conjunction with the drawings of the invention will be further described.

[0013] 实施例1 [0013] Example 1

[0014] 如图1所示,基于不同波长激发的自体荧光检测系统图。 [0014] As shown in FIG. 1, FIG autologous fluorescence detection system based on different excitation wavelengths. 选取一例人体结肠组织进行自体荧光光谱特性研究。 Select one case human colon tissue autologous fluorescence spectral characteristics. 在进行实验之前,通过冷却电压控制器(12)将冷却器(13) (C9144, Hamamatsu Corp.,Japan)开启并冷却30 分钟,使光电倍增管(14) (R928, Hamamatsu Corp.,Hamamatsu, Japan)达到稳定工作状态。 Before the experiment, (12) a cooler (13) (C9144, Hamamatsu Corp., Japan) was cooled opened by a voltage controller and cooled for 30 minutes to a photomultiplier tube (14) (R928, Hamamatsu Corp., Hamamatsu, Japan) reach steady state operation. 根据实验要求,打开计算机(18),并预先存储了与结肠组织相对应的三个阈值In和IT2,其数值分别为30000和3,打开可调谐激光器(1) (PIV OPO 355,0Ρ0ΤΕΚ, USA),通过计算机(18)上的控制软件调节可调谐激光器(1)的输出波长为400nm,同时控制选择滤光轮(10) (FW102B, Thorlabs Inc., USA)上中心波长为635nm的滤光片。 According to the experimental requirements, turn on the computer (18), and colon tissue with previously stored corresponding to the three threshold values ​​In and IT2, which values ​​are 30,000 and 3, opening tunable laser (1) (PIV OPO 355,0Ρ0ΤΕΚ, USA ), by controlling software on a computer (18) adjusting the tunable laser (1) an output wavelength of 400 nm, while controlling the selection filter wheel (10) (FW102B, center) Thorlabs Inc., USA 635nm wavelength filter sheet. 可调谐激光器(1)发出的激发光经过光衰减器(2) 进行功率调节,之后经过耦合透镜(3)耦合并进入传输激发光光纤的激发光输入端(4)上的六根传输激发光的光纤。 Excitation tunable laser (1) emits light through the optical attenuator (2) for power regulation, after the coupling lens (3) is coupled and enters the excitation transfer excitation light fiber light input six transmission on the end (4) of the excitation light optical fiber. 传输激发光的光纤接入端(5)通过内窥镜(6) (CF-1T140I/L, Olympus Corp.,Japan)将激发光通过活检通道均勻照射在待测组织(7)上,待测组织(7) 被激发后发出的荧光再由Y型光纤中的传输荧光的光纤接入端收集,最后从Y型光纤的荧光输出端(8)输出。 Fiber access terminal (5) of the excitation light transmitted by the endoscope (6) (CF-1T140I / L, Olympus Corp., Japan) uniformly irradiated by the excitation light in a tissue biopsy channel (7) to be tested, the test tissue (7) fluorescence emitted by the excited fluorescence re Y-fiber transmission fiber access terminal collection, the final output from the output terminal Y type fluorescent optical fiber (8). 输出的荧光由前耦合透镜(9)耦合后经过滤光轮(10)上中心波长为635nm的滤光片,进行窄带滤波后,再由后耦合透镜(11)耦合到光电倍增管(14),通过光电倍增管(14)光电转化后转换为电信号,经过前置放大器(15) (C6438,Hamamatsu Corp., Hamamatsu, Japan)进行电信号放大,放大后的电信号再由单光子计数器(16) (C9744, Hamamatsu Corp. , Hamamatsu, Japan)鉴另U后通过计数板卡(17) (M8784, Hamamatsu Corp., Hamamatsu, Japan)进行计数并由计算机(18)记录635nm的荧光信号I635,其数值为10000, 小于阈值IT1,未能判定组织类型,须进行进一步检测。 Fluorescence output by the front coupling lens (9), after coupling the filter wheel (10) of the filter center wavelength of 635nm, after narrowband filtering, then after coupling the coupling lens (11) to a photomultiplier tube (14) , converted by the photomultiplier tube (14) the photoelectric conversion electrical signals, via a preamplifier (15) (C6438, Hamamatsu Corp., Hamamatsu, Japan) amplified electrical signal, and then the amplified electric signal by a single photon counter ( 16) after (C9744, Hamamatsu Corp., Hamamatsu, Japan) Kam another U counted by a computer (18) recording the fluorescence of 635nm signal I635 by counting the board (17) (M8784, Hamamatsu Corp., Hamamatsu, Japan), a value of 10 000, is less than the threshold value IT1, failure determining tissue type shall further testing. 再通过计算机(18)上的控制软件调节可调谐激光器(1)的输出波长为280nm,同时控制选择中心波长为335nm的滤光片,由计数板卡(17)进行计数并由计算机(18)记录335nm的荧光信号I335,其数值为96000 ;再通过计算机(18)上的控制软件调节可调谐激光器(1)的输出波长为340nm,同时控制选择中心波长为390nm的滤光片,由计数板卡(17)进行计数并由计算机(18)记录390nm的荧光信号I39。 Reconditioning tunable laser by controlling software on a computer (18) (1) an output wavelength of 280 nm, while controlling the center wavelength of 335nm selected filter, counted by the board (17) counted by the computer (18) i335 335nm recorded fluorescence signal, a value of 96,000; reconditioning tunable laser (1) an output wavelength of 340nm by controlling software on a computer (18), while controlling the center wavelength of 390nm selected filter by plate count card (17) can be counted by a computer (18) records the fluorescent signal I39 390nm. ,其数值为50000 ;再选择中心波长为460nm的滤光片,记录460nm的荧光信号146(1, 其数值为82000。再选择可调谐激光器(1)输出波长为450nm,同时选择中心波长为535nm 的滤光片检测535nm的荧光信号I535,其数值为15000。最后,再选择可调谐激光器(1)输出波长为460nm,同时选择中心波长为520nm的滤光片检测520nm的荧光信号152(1,其数值为20000。在计算机(18)上进行数据处理,计算Ir = (I46O+I52O+I535)XI335Z(I39O)2的结果为4. 49,大于阈值It2,表明还原型烟酰胺腺嘌呤二核苷酸,黄素腺嘌呤二核苷酸和色氨酸相对于胶原蛋白的相对含量较高,判定待测组织为结肠肿瘤组织。[0015] 实施例2 , A value of 50000; then select a filter center wavelength 460nm, 460nm fluorescence signal record 146 (1, then its value is 82000. selection tunable laser (1) an output wavelength of 450 nm, a center wavelength of 535nm are simultaneously selected detection 535nm filter I535 fluorescent signal, a value of 15000. Finally, select tunable laser (1) an output wavelength of 460 nm, a center wavelength of the filter while selecting the 520nm 520nm fluorescence detection signal 152 (1, has a value of 20000. performed on the computer (18) data processing, computing Ir = (I46O + I52O + I535) XI335Z (I39O) results for 4.49, It2 is greater than a threshold, indicates that reduced nicotinamide adenine dinucleotide nucleotide, flavin adenine dinucleotide, and tryptophan with respect to the relatively high content of collagen tissue to be tested is determined colon tumor tissue. [0015] Example 2

[0016] 选取另一例人体结肠组织进行自体荧光光谱特性研究。 [0016] Select another embodiment human colon tissue autologous fluorescence spectral characteristics. 按照实施例1的过程,首先选择400nm激发光,检测635nm的荧光信号I635,其记录的数值为9000,小于阈值IT1,未能判定组织类型,须进行进一步检测。 According to the procedure of Example 1, first select the 400nm excitation light, the fluorescent signal is detected I635 635nm, values ​​which record is 9000, is less than the threshold value IT1, failure determining tissue type shall further testing. 接着,选择280nm激发光激发,检测335nm的荧光信号I335,其数值为75000。 Next, select the 280nm excitation light, the fluorescent signal is detected I335 335nm, a value of 75,000. 然后选择340nm激发光,检测390nm和460nm的荧光信号I39tl和146(1, 其数值分别为70000和81000。再选择450nm激发光激发,检测535nm的荧光信号I535,其数值为10000。最后,选择460nm激发光,检测520的荧光信号I52tl,其数值为18000。计算Ir = (I46O+I52O+I535)XI335Z(I39O)2的结果为I- 67,小于阈值IT2,表明表明还原型烟酰胺腺嘌呤二核苷酸,黄素腺嘌呤二核苷酸和色氨酸相对于胶原蛋白的相对含量较低,判定待测组织为结肠正常组织。 Then select the 340nm excitation light, and detecting the fluorescence signals I39tl 460nm 390nm and 146 (1, its value respectively 70,000 and 81000. reselection 450nm excitation light, the fluorescent signal is detected I535 535nm, which is a value of 10000. Finally, select 460nm excitation light, the fluorescent signal is detected I52tl 520, calculates a value of 18000. Ir = (I46O + I52O + I535) XI335Z (I39O) results of I- 67, IT2 less than a threshold, indicates that show reduced nicotinamide adenine dinucleotide , flavin adenine dinucleotide, and tryptophan with respect to relatively low collagen content, determined as normal colon tissue tested tissue.

[0017] 实施例3 [0017] Example 3

[0018] 选取另一例人体结肠组织进行自体荧光光谱特性研究。 [0018] Select another embodiment human colon tissue autologous fluorescence spectral characteristics. 按照实施例1的过程,首先选择400nm激发光,检测635nm的荧光信号I635,其记录的数值为60000,高于阈值In,表明卟啉含量较高,判定待测组织为结肠肿瘤组织。 Following the procedure of Example 1, first select 400nm excitation light, the fluorescent signal is detected I635 635nm, values ​​which recorded 60,000, higher than the threshold value In, indicating a higher content of a porphyrin, as determined tissue colon tumor tissues tested.

Claims (4)

  1. 一种基于不同波长光源激发的人体组织自体荧光检测系统,主要由激发光源部分、激发‑检测‑采集部分、光学收集部分、信号预处理部分和计算机部分构成,其中信号预处理部分与计算机部分通过信号线进行连接,激发光源部分通过激发光与激发‑检测‑采集部分中的激发光输入端的光纤相连,激发‑检测‑采集部分中的荧光输出端的光纤通过荧光信号通路与光学收集部分相连,荧光信号通路经过光学收集部分传输后进入信号预处理部分,信号预处理部分中光电倍增管光电转化后变为电信号,进入计算机部分,其特征在于可调谐激光器(1)可以根据人体组织中特定的内源性荧光物质调节所需的脉冲激光波长,滤光轮(10)上安装有不同中心波长的窄带滤光片。 Based on human tissue autofluorescence detection systems excitation light sources of different wavelengths, mainly by the excitation light source section, excitation - detection - acquisition part, an optical collection portion, and a computer signal preprocessing section parts, wherein the signal pre-processing section by the computer portion a signal line connected to the excitation light source section through the excitation light and excitation - detection - excitation light input acquisition part of the optical fiber is connected, excitation - detection - fluorescence output terminal collection portion of the optical fiber by the fluorescent signal paths connected to the optical collecting portion, fluorescence after the optical signal path into the collection part of the transmission signal preprocessing, signal preprocessing section photomultiplier tube after the photoelectric conversion into electrical signals, into the computer portion, characterized in that the tunable laser (1) according to a specific human tissue endogenous fluorescent substance adjust the desired laser wavelength pulse, narrowband filter is mounted on a different center wavelength of the filter wheel (10).
  2. 2.根据权利要求1所述的一种基于不同波长光源激发的人体组织自体荧光检测系统, 其特征在于所述的可调谐激光器可依次调节的波长范围从210nm到2200nm。 According to one of the claims 1 to body tissue autofluorescence-based detection systems of different wavelengths of excitation light, wherein the wavelength range of the tunable laser may be sequentially adjusted from 210nm to 2200nm.
  3. 3.根据权利要求1所述的一种基于不同波长光源激发的人体组织自体荧光检测系统, 其特征在于所述的滤光轮上安装有不同中心波长的窄带滤光片。 According to one of the claims 1 to body tissue autofluorescence-based detection system of excitation light sources of different wavelengths, characterized in that the filter is mounted on the wheel of a different narrowband filter center wavelength.
  4. 4.根据权利要求1所述的一种基于不同波长光源激发的人体组织自体荧光检测系统, 其特征在于可以通过计算机上的同步控制软件,实现可调谐激光器输出的激发光波长与滤光轮上相对应的窄带滤光片的同步转换。 According to one of the claims 1 fluorescence detection system based on a different human tissues autologous excitation wavelength of the source, characterized by the synchronization control software on a computer, implement the tunable laser output wavelength of the excitation light and the filter wheel synchronous conversion corresponding to the narrow band filter.
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