CN101948164A - Biological membrane quick-constructing method used in seawater factory breeding cycle water purification system - Google Patents
Biological membrane quick-constructing method used in seawater factory breeding cycle water purification system Download PDFInfo
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- CN101948164A CN101948164A CN2010102789362A CN201010278936A CN101948164A CN 101948164 A CN101948164 A CN 101948164A CN 2010102789362 A CN2010102789362 A CN 2010102789362A CN 201010278936 A CN201010278936 A CN 201010278936A CN 101948164 A CN101948164 A CN 101948164A
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Abstract
The invention relates to a biological membrane quick-constructing method used in a seawater factory breeding cycle water purification system. The biological membrane is composed of SZ colloidal composites and combination purification bacteria, wherein the SZ colloidal composites are prepared by mixing yellow clay with zeolite powder; the combination purification bacteria is composed of four strains of low temperature resistant water purification bacteria, including bacillus subtilis, bacillus amyloliquefaciens and the like; and the biological membrane with a stable structure can be constructed by splashing the SZ colloidal composites and the combination purification bacteria together into a biological tank containing biological carriers and then ventilating for 4-5d to form the membrane. After testing, the biological membrane has good water purification effect on breeding waste water. The removal rate on ammonia nitrogen, nitrate, nitrite and COD (Chemical Oxygen Demand) in water body after passing the biological purification tank can respectively reach 23%, 72.6%, 73% and 78.3%. The biological membrane quick-constructing method has the advantages of simple method, short constructing period and good water quality purification effect, and is suitable for constructing biological filtering tanks in large-scale seawater factory breeding cycle water systems.
Description
Technical field:
The present invention belongs to make up biomembranous technology in the seawater industrialized culture recirculating water purification system fast, is a kind of quick structure microbial film method of carrying out recirculating water purification in seawater industrialized culture purification system.
Background technology:
In recent years, along with the raising of cultural technique level, China's sea farming industry is developed rapidly, the development situation of many kinds, multi-form, many industrial zones occurred, has produced huge economic and social benefit.The sea farming mode mainly contains three kinds at present, and hydrostatic is cultured, flowing water is cultured and circulating water cultivation.Hydrostatic is cultured and is applicable to pond culture, and the pond area is big, and day quantity of exchanged water is less relatively; Flowing water is cultured and to be applicable to little water body, such as small-sized intensive culture pond, indoor industrially-cultured etc.The above two, very big to water resource waste, and can cause the ecological pollution of environment.Circulating water cultivation then makes one of its important directions that becomes the culture fishery development with advantages such as " energy-saving and environmental protection, sustainable ".Circulating water cultivation has a whole set of self-contained water treating equipment, form comprising facilities and equipments such as Microfilter (or sieve-bend screen), protein separator, biopurification pond, air supporting degasifying device, comprehensive temperature adjustment pond, highly effective oxygen dissolving device, ultraviolet sterilization apparatus, the on-line automatic monitoring devices of water quality, through removing, reach the purpose that makes water purification and recycle to objectionable impuritiess such as solid particulate, colloidal solid, dissolved organic matter, ammonia nitrogen, obnoxious flavour and pathogenic microorganism in the water.Its core is the biopurification pond, biomembranous formation and the good and bad gentle ability of purifying waste water of circular water treating system that is directly connected in the biopurification pond.
Sophisticated microbial film has very strong adsorptive power, when waste water is flowed through microbial film, organism and " ternary nitrogen " etc. in the microbial film planar water, because the biomembrane purification method is environmentally friendly, expense is cheap, being applicable to various labile water area conditions, is the Pollution control technology of rising " green " sea farming.
Yet present bio-film colonization technology is also very backward, generally all is to adopt the natural condition biofilm.The natural membrane method need shift to an earlier date 40 days and carry out, and construction schedule is long, has influenced the breeding production plan; Cultivate during the microbial film, also do not carry and breed fish, equipment idle running having increased cost; In addition, easily give birth to assorted bacterium, pathogenic bacteria, lack purpose bacterium with good purification function.Do not have biomembranous circulating water system just not have good water treatment effect, in other words, the microbial film constructing technology becomes the conditionality factor that current industrial circulating water cultivating mode development is promoted.
For this reason, set up a kind of have simple to operate, the biofilm time is short, with low cost, the rapid biofilm technological method of good purification, is the task that industry is badly in need of, it also will have wide application and promotion prospect.At present, Shang Weijian has the research report of this aspect.
Summary of the invention:
The purpose of this invention is to provide and a kind ofly can in the seawater industrial circulating water cultivating system, make up biomembranous technological method fast, to solve long, deficiencies such as pollution easily of traditional natural membrane cycle.Can make up effective microbial film fast by making in this way, constructed microbial film has the ideal decontamination effect improving to aquaculture wastewater.
The present invention realizes that by the following method its step comprises: 1, microbial film film base is the preparation of SZ glue compound: 2, the preparation of combination purification bacterium; 3, biomembranous quick structure.
1, microbial film film base is the preparation of SZ glue compound: it is made up of yellow clay of ultra micro and zeolite powder, and yellow clay and zeolite powder are pressed 4: 1 mixed of mass ratio composition after 200 order yarn thin,tough silk filter; Concentration according to 5g/L is dissolved in clay and zeolite powder mixture in the seawater again, fully the colloidal solution that forms behind the aeration mixing, i.e. SZ glue compound.
2, the preparation of combination purification bacterium: the combination purification bacterium is subtilis Bacillus subtilis, bacillus amyloliquefaciens Bacillus amyloliquefaciens, nitrated spirillum Nitrospira sp., have a liking for the low temperature resistant purification of water quality bacterium of salt Zymomonas mobilis Halomon sp. four strains and form, respectively to the 4 strain purification of water quality bacterium enlarged culturing 24～36h that vibrates, make its bacterial strain final concentration reach 10 with the pancreas peptone soybean broth substratum
9CFU/ml carries out 4 strain purification of water quality bacterium equal proportion then and mixes, and preparation becomes the combination purification bacterium.
3, biomembranous quick structure: behind " SZ glue compound " logical ozonization 20min, throw in the breeding wastewater of bio-carrier with the combination purification bacterium, wherein " SZ glue compound " consumption is every cube of water body with 4～5L, and the final concentration of each bacterial strain of purification of water quality bacterium is 10
3CFU/ml, fully aeration was cultivated 4～5 days, can see that more yellow dirt settling is arranged on the bio-carrier, and microbial film makes up and finishes.
The present invention and prior art contrast its characteristics:
1, the invention belongs to biomembranous quick structure in the seawater circulating treating system, this method is applicable to that breeding wastewater is handled under high temperature and the low temperature, particularly under cold condition, bacterium is difficult to growth and breeding especially, the present invention has screened the purification of water quality bacterium that cold condition down can growth and breeding, makes under the low temperature biomembranous to be built into for reality fast.
2, the used time of the present invention is short, can finish biomembranous structure in 4-5 days, and compare, shorten the time greatly with traditional colonization method, and working method is simple, with low cost, have no side effect, and goes for the structure of biological filter in the extensive water circulation system.
3, present method has been created quick structure technology of biological membrane first, and adapting to current is the flounder flounder class circulating water cultivation pattern at water source with the missile silo seawater, provides theoretical foundation and technical support to promoting the closed circulation water breed.
Embodiment:
Application Example below by in source, sky, development area, Yantai aquatic products company limited is described in detail construction process of the present invention:
Its method steps comprises: 1, microbial film film base is the preparation of SZ glue compound: 2, the preparation of combination purification bacterium; 3, biomembranous quick structure.
Biological filter of design in the closed circulation water turbot cultivating system of source, sky, the filter tank size is 36m * 1.8m * 1.7m, before the biofilm of biological filter, the bio-carrier vertical lifting is suspended in the filter tank, uses KMNO
4About 100m is injected in the sterilization back in the biological filter
3Seawater.
1, microbial film film base is the preparation of SZ glue compound:
Clay is taken from the clay of irrigation canals and ditches bottom, Dongying City somewhere, and it is yellow that ashen is, and soil property viscosity is bigger, and grit content is few.After obtained clay drying, pulverize with supper micron mill, filter with 200 order yarn thin,tough silk, obtain the clay of micronizing, again with the zeolite powder of buying on the market, filter with 200 order yarn thin,tough silk, obtain the zeolite powder of micronizing, according to quality than clay: the ratio of zeolite powder=4: 1 is with clay and the abundant mixing of zeolite powder.At volume is 1m
3Plastic barrel in inject the 500L seawater, the mixture that adds 2.5kg clay and zeolite powder simultaneously, powerful ventilation makes water stir 1d and is prepared into suspension colloid, i.e. " SZ glue compound " (concentration is 5g/L), this SZ glue compound can form a loose environment after being attached on the bio-carrier, helps adhering to of bacterium.And this colloid can also make bacterium to grow in the above for bacterium provides nutrition.
2, the preparation of combination purification bacterium:
The combination purification bacterium is made up of the low temperature resistant purification of water quality bacterium of four strains that obtains through a large amount of experiment screenings, they are respectively subtilis Bacillus subtilis, bacillus amyloliquefaciens Bacillus amyloliquefaciens, nitrated spiral shell Nitrospira sp., have a liking for salt Zymomonas mobilis Halomonas sp..Each bacterial strain mainly acts on: subtilis can transform and absorb nitrogen element in aquaculture water or the various sewage body, decomposing organic matter and reduction COD; Bacillus amyloliquefaciens can degradation of ammonia nitrogen; Nitrated spirillum can degrading nitrite; Having a liking for the salt unit cell can decomposing organic matter and reduce COD;
With the pancreas peptone soybean broth substratum the four strain purification of water quality bacterium shaking culture 24h that obtain are separated in the laboratory, make its concentration reach 10
9CFU/ml is prepared into the combination purification bacterium with four strain bacteriums according to the equal proportion mixing.
3, biomembranous quick structure:
" SZ glue compound " static 20min with preparing in the above-mentioned steps goes its precipitation, and logical ozonization 20min adds 100mL combination purification bacterium liquid to about 500L colloidal solution, fully splashes in biological tank behind the stirring and evenly mixing, and consumption is every m
3Water body is with 4～5L " SZ glue compound ", and the final concentration of each purification of water quality bacterium is 10
3CFU/ml.Splash finish after, fully aeration is cultivated.Observe the last film situation of " SZ glue compound " every day, comprises water purification flushing, white paper towels wiping, microscopic examination.In the process of the test, visible white transparence bio-carrier surface had faint yellow material to adhere in the 1st～2 day, but insecure, was easy to rinse well when washing with water purification.Test proceeds to the 4th day, can see that yellow dirt settling is more firm, flaxen material is still arranged attached to the bio-carrier surface after the water purification flushing, and under the well-lighted situation, naked eyes can see that slick carrier surface becomes coarse.Test proceeds to the 5th day, can see that yellow bur is more on the needle-like bio-carrier, and water purification dashes can't to be washed off, use the white paper towels wiping, can see obviously having yellow vestige to stay on the paper handkerchief, microscopic examination can find that carrier surface has the xanchromatic particulate material, indicates that biofilm finishes.
After the biofilm maturation, open a whole set of circulating water culture system, circulation process is that breeding wastewater is removed macrobead suspended substances such as residual bait, ight soil through Microfilter, again after ozone-Protein Skimmer is isolated short grained solubility and non-solubility suspended substance, enter the biological filter and remove water-soluble objectionable impuritiess such as ammonia nitrogen, nitrite nitrogen and COD, behind ultraviolet radiation for sterilizing and disinfecting, enter the breed pond and carry out recycle.Recycle system operation 1 month, after each condition of water quality is stable, the ammonia nitrogen of detection of biological filter tank intake-outlet, nitrate, nitrite and COD content, draw the constructed microbial film of present method the clearance of culturing ammonia nitrogen, nitrate, nitrite and COD in the recirculated water is reached 23%, 72.6%, 73% and 78.3% (table 1) respectively, effects of purification quality is obvious.
Present method has been created quick structure technology of biological membrane first, shortened incubation time greatly, working method is simple, with low cost, adapting to current is the flounder flounder class circulating water cultivation pattern at water source with the missile silo seawater, provide theoretical foundation and technical support to promoting the closed circulation water breed, had important practical significance for promoting aquaculture technology.
The microbial film effects of purification quality that table 1 present method is constructed
| Water-quality guideline | The biological filter water-in | The biological filter water outlet | Clearance |
| Ammonia nitrogen mg/L | 0.156 | 0.12 | 23％ |
| Nitrite mg/L | 0.063 | 0.017 | 73％ |
| Nitrate mg/L | 0.73 | 0.20 | 72.6％ |
| COD?mg/L | 13.94 | 3.02 | 78.3％ |
Claims (1)
1. microbial film fast construction method in the seawater industrialized culture recirculating water purification system, it is characterized in that its method steps comprises: (1), microbial film film base are the preparation of SZ glue compound: the preparation of (2), combination purification bacterium; (3), biomembranous quick structure;
(1), microbial film film base is the preparation of SZ glue compound: it is made up of yellow clay and zeolite powder, with yellow clay and zeolite powder micronizing, presses 4: 1 mixed of mass ratio and form after 200 order yarn thin,tough silk filter; Concentration according to 5g/L is dissolved in clay and zeolite powder mixture in the seawater again, fully the colloidal solution that forms behind the aeration mixing, i.e. SZ glue compound;
(2), the preparation of combination purification bacterium: the combination purification bacterium is by subtilis Bacillus subtilis, bacillus amyloliquefaciens Bacillus amyloliquefaciens, nitrated spirillum Nitrospira sp., have a liking for the low temperature resistant purification of water quality bacterium of salt Zymomonas mobilis Halomonas sp. four strains and form, respectively to the 4 strain purification of water quality bacterium enlarged culturing 24～36h that vibrates, make its bacterial strain final concentration reach 10 with the pancreas peptone soybean broth substratum
9CFU/ml carries out 4 strain purification of water quality bacterium equal proportion then and mixes, and preparation becomes the combination purification bacterium;
(3), biomembranous quick structure: behind " SZ glue compound " logical ozonization 20min, throw in the breeding wastewater of bio-carrier with the combination purification bacterium, wherein " SZ glue compound " consumption is every cube of water body with 4～5L, and the final concentration of each bacterial strain of purification of water quality bacterium is 10
3CFU/ml, fully aeration was cultivated 4～5 days, can see that more yellow dirt settling is arranged on the bio-carrier, and microbial film makes up and finishes.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102948380A (en) * | 2011-08-24 | 2013-03-06 | 中国水产科学研究院黄海水产研究所 | Energy-saving factory circulating water oplopanaxelatus seedling protection process method |
| CN103160453A (en) * | 2013-02-26 | 2013-06-19 | 武汉施瑞福生物技术有限公司 | Preparation method of microecologics for nitrite degradation, microecologics and use thereof |
| CN103979671A (en) * | 2014-05-21 | 2014-08-13 | 河海大学 | T-stance mountain river water purification method |
| CN116177804A (en) * | 2023-02-28 | 2023-05-30 | 浙江省淡水水产研究所 | Water quality regulation and control system and method for recirculating aquaculture of micropterus salmoides |
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| CN1151085C (en) * | 2002-03-22 | 2004-05-26 | 清华大学 | Process for treating sewage with zeolite filler in aerated biologic filter pool and zeolite filler |
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2010
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Patent Citations (4)
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| JP2001239136A (en) * | 2000-03-02 | 2001-09-04 | Nitto Denko Corp | Treating system and operating method therefor |
| CN1151085C (en) * | 2002-03-22 | 2004-05-26 | 清华大学 | Process for treating sewage with zeolite filler in aerated biologic filter pool and zeolite filler |
| CN1202019C (en) * | 2003-01-17 | 2005-05-18 | 清华大学 | Waste brick application in waste water treatment |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102948380A (en) * | 2011-08-24 | 2013-03-06 | 中国水产科学研究院黄海水产研究所 | Energy-saving factory circulating water oplopanaxelatus seedling protection process method |
| CN103160453A (en) * | 2013-02-26 | 2013-06-19 | 武汉施瑞福生物技术有限公司 | Preparation method of microecologics for nitrite degradation, microecologics and use thereof |
| CN103979671A (en) * | 2014-05-21 | 2014-08-13 | 河海大学 | T-stance mountain river water purification method |
| CN116177804A (en) * | 2023-02-28 | 2023-05-30 | 浙江省淡水水产研究所 | Water quality regulation and control system and method for recirculating aquaculture of micropterus salmoides |
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Application publication date: 20110119 Assignee: Haiyang the Yellow Sea aquatic products Co., Ltd., Shandong Assignor: Inst of Huanghai Sea Marine Products, Chinese Academy of Aquatic Product Science Contract record no.: 2013370000010 Denomination of invention: Biological membrane quick-constructing method used in seawater factory breeding cycle water purification system Granted publication date: 20120411 License type: Exclusive License Record date: 20130129 |
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