CN101940349B - A kind of corn silk mineral water and preparation method thereof - Google Patents

A kind of corn silk mineral water and preparation method thereof Download PDF

Info

Publication number
CN101940349B
CN101940349B CN201010256249A CN201010256249A CN101940349B CN 101940349 B CN101940349 B CN 101940349B CN 201010256249 A CN201010256249 A CN 201010256249A CN 201010256249 A CN201010256249 A CN 201010256249A CN 101940349 B CN101940349 B CN 101940349B
Authority
CN
China
Prior art keywords
corn stigma
mineral water
content
water
corn
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201010256249A
Other languages
Chinese (zh)
Other versions
CN101940349A (en
Inventor
刘吉成
关宏
杨文钦
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhang Guojun
Original Assignee
Qiqihar Medical University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiqihar Medical University filed Critical Qiqihar Medical University
Priority to CN201010256249A priority Critical patent/CN101940349B/en
Publication of CN101940349A publication Critical patent/CN101940349A/en
Application granted granted Critical
Publication of CN101940349B publication Critical patent/CN101940349B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Landscapes

  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses corn stigma mineral water. The corn stigma mineral water is prepared from corn stigma and mineral water serving as raw materials, wherein the weight-to-volume ratio of the corn stigma to the mineral water is 0.1 to 1kg to 100L; the polysaccharide content of the corn stigma mineral water is 0.01 to 0.5g/L; the total saponin content is 0.01 to 0.1g/L; and the total flavone content is 0.01 to 0.1g/L. The invention also discloses a preparation method of the corn stigma mineral water; and the method comprises the following steps of: placing the corn stigma into a sealed container; adding preheated mineral water into the container; performing thermal extraction and solid-liquid separation; performing secondary filtration on the liquid part, sterilizing the liquid part at a high temperature and cooling the liquid part to 90 DEG C for thermal encapsulation; and pouring the liquid part into bottles, cooling the liquid part by spraying, performing visual inspection and packaging the bottles to obtain the corn stigma mineral water. The corn stigma mineral water does not have any preservative and has the health-care functions of antioxidation, diuresis, pressure lowering, blood sugar level regulation, enhancement of immunity of a body and the like, so that the corn stigma mineral water can replenish moisture required by human body every day in place of drinking water and can be used as a functional beverage for diabetic patients to play a part in prevention and health care.

Description

一种玉米须矿泉水及其制备方法A kind of corn silk mineral water and preparation method thereof

技术领域 technical field

本发明涉及一种玉米须矿泉水及其制备方法,属于保健饮料加工领域。The invention relates to corn silk mineral water and a preparation method thereof, belonging to the field of health beverage processing.

背景技术 Background technique

玉米须(stigma maydis)为禾本科玉蜀属植物玉米的花柱和柱头。在我国,玉米须作为中药材已有悠久的临床用药历史,研究表明,玉米须中含有多种生物活性成分,例如黄酮及其苷类、甾醇、生物碱、糖类、有机酸、挥发油、微量元素及多种维生素等,其提取物具有抗氧化、利尿、降血糖、抑菌、降压和增强免疫等功效。美国食品药物管理局确认玉米须为安全、无毒的物质。Corn silk (stigma maydis) is the style and stigma of maize, a plant of the genus Maize in the grass family. In my country, corn silk has a long history of clinical use as a traditional Chinese medicine. Studies have shown that corn silk contains a variety of biologically active components, such as flavonoids and their glycosides, sterols, alkaloids, sugars, organic acids, volatile oils, trace amounts Elements and multivitamins, etc., its extracts have anti-oxidation, diuretic, hypoglycemic, antibacterial, antihypertensive and immune-enhancing effects. The US Food and Drug Administration has confirmed that corn silk is a safe, non-toxic substance.

目前,玉米须的应用主要是作为原料与其它中药材进行组方,剂型多为冲剂、滴丸剂、丸剂、片剂、胶囊剂、颗粒剂、软胶囊剂、散剂或口服液剂型,主要用于糖尿病和高血压等疾病的治疗。玉米须提取物的获得主要是采用乙醇回流提取、水煎煮法提取或超声波提取、再经浓缩、精制和干燥而获得。制备方法复杂,玉米提取物中成分单一,挥发性物质丢失严重,造成天然的玉米风味的损失。At present, the application of corn silk is mainly used as a raw material and other traditional Chinese medicinal materials for formulating prescriptions. Treatment of diseases such as diabetes and high blood pressure. Corn silk extract is mainly obtained by ethanol reflux extraction, water decoction extraction or ultrasonic extraction, followed by concentration, refining and drying. The preparation method is complicated, the ingredients in the corn extract are single, the volatile substances are seriously lost, and the natural corn flavor is lost.

近年来,随着人们生活水平的不断提高、膳食结构的改变,糖尿病(diabeticmellitus,DM)的发病率逐年提高,严重地影响了人们的生活品质。特别是二型糖尿病(type 2 diabetes mellitus,T2DM),一般在成年人(35-45岁)中发病,随着年龄增加,其发病率不断提高,占糖尿病总发病率的90%以上,是临床上最常见、危害最大的内分泌疾病之一。二型糖尿病的病情一般较缓和,隐蔽,其治疗方法以运动和饮食控制为主。因此,健康饮食对于预防糖尿病的发生是极为重要的。刘吉成教授课题组利用二型糖尿病大鼠模型研究了玉米须水浸提物的生理功能发现:1)玉米须水浸提物能够显著降低空腹血糖和胰岛细胞中胰高糖素的表达(温宪春,周丽,赵健飞,刘吉成,玉米须水提物对2型糖尿病大鼠胰高糖素的影响,医学研究杂志2010,39(4):52-54);2)玉米须水提物对损伤的胰岛β-细胞具有一定的修复和保护作用(温宪春,李雪岩,李晓明,李成冲,刘吉成,玉米须水提物对2型糖尿病大鼠胰岛β-细胞的保护作用,中国中医药信息杂志2010,17(2):37-39);3)玉米须水提物可改善2型糖尿病大鼠糖-脂代谢,抑制氧化应激反应(温宪春,周丽,李晓明,李成冲,刘吉成,玉米须水提物对2型糖尿病大鼠糖-脂代谢及氧化应激的影响,中国中医药信息杂志2010,17(5))。In recent years, with the continuous improvement of people's living standards and changes in dietary structure, the incidence of diabetes mellitus (DM) has increased year by year, seriously affecting people's quality of life. Especially type 2 diabetes mellitus (T2DM), which generally occurs in adults (35-45 years old), and its incidence rate continues to increase with age, accounting for more than 90% of the total incidence of diabetes. One of the most common and harmful endocrine diseases in the world. The condition of type 2 diabetes is generally mild and hidden, and its treatment methods are mainly exercise and diet control. Therefore, a healthy diet is extremely important to prevent the occurrence of diabetes. Professor Liu Jicheng's research group used the rat model of type 2 diabetes to study the physiological functions of the water extract of corn silk and found that: 1) the water extract of corn silk can significantly reduce the expression of fasting blood sugar and glucagon in islet cells (Wen Xianchun , Zhou Li, Zhao Jianfei, Liu Jicheng, Effect of corn silk water extract on glucagon in type 2 diabetic rats, Journal of Medical Research 2010, 39(4):52-54); 2) corn silk water extract on Damaged islet β-cells have certain repairing and protective effects (Wen Xianchun, Li Xueyan, Li Xiaoming, Li Chengchong, Liu Jicheng, Protective effect of corn silk water extract on islet β-cells of type 2 diabetic rats, Chinese Journal of Traditional Chinese Medicine Information 2010 , 17(2):37-39); 3) Corn silk water extract can improve glucose-lipid metabolism and inhibit oxidative stress in type 2 diabetic rats (Wen Xianchun, Zhou Li, Li Xiaoming, Li Chengchong, Liu Jicheng, Corn silk Effects of water extract on glucose-lipid metabolism and oxidative stress in type 2 diabetic rats, Chinese Journal of Traditional Chinese Medicine Information 2010, 17(5)).

因为玉米须水提液有很好的保健功效,人们开始开发以玉米须为主要原料的保健饮品,例如中国专利号ZL 96121424.4公开了“一种植物保健饮料及其制作工艺”和中国专利公开号CN101396161A公开了“一种木糖醇玉米须醋饮料及其制作方法”,但是这些专利申请在配料方面,除玉米须以外,还配有其它成分,大多数还都添加了防腐剂,这些成份掩盖了玉米须特有的清香口味;同时玉米须水提液制备方法均采用煮沸的方法,使玉米须主体香味物质丢失严重,影响了产品的口感。Because the corn silk water extract has a good health care effect, people begin to develop health drinks with corn silk as the main raw material. CN101396161A discloses "a kind of xylitol corn silk vinegar beverage and its preparation method", but these patent applications are also equipped with other ingredients except corn silk in ingredients, and most of them also add preservatives, and these ingredients cover up The unique fragrance taste of corn silk is obtained; at the same time, the preparation method of corn silk water extract all adopts the method of boiling, so that the main aroma substance of corn silk is seriously lost, and the taste of the product is affected.

D-塔格糖有着很重要的保健功能。目前的研究结果表明,1)D-塔格糖是一种很好的低热量、填充型甜味剂。D-塔格糖的甜度为蔗糖的92%,而产生的热量只为蔗糖的1/3,并且和蔗糖的味道十分相似;2)人体吸收率低,人体摄入的D-塔格糖中只有20%被小肠吸收,不会引起机体血糖水平的明显变化,很适合糖尿病人食用;3)D-塔格糖是益生素。大部分不被吸收的D-塔格糖在结肠中被一些微生物菌群发酵利用,产生短脂肪酸链,促进有益菌增殖;4)不易被口腔细菌分解利用;因此D-塔格糖具有低能量、降血糖、预防肥胖、改善肠道菌群和抗龋齿等多种生理功能。D-塔格糖除了作为甜味剂之外,还可作为食品的风味增强剂。目前,大量用于健康饮料、酸奶、果汁、焙烤食品、糖果和医药制剂(如糖浆剂、咀嚼片)等产品中,作为蔗糖的替代品。D-tagatose has very important health functions. The current research results show that 1) D-tagatose is a good low-calorie, bulking sweetener. The sweetness of D-tagatose is 92% of that of sucrose, and the heat generated is only 1/3 of that of sucrose, and the taste is very similar to that of sucrose; Only 20% of it is absorbed by the small intestine, which will not cause significant changes in the blood sugar level of the body, and is very suitable for diabetics; 3) D-tagatose is a prebiotic. Most of the unabsorbed D-tagatose is fermented and utilized by some microbial flora in the colon to produce short fatty acid chains and promote the proliferation of beneficial bacteria; 4) it is not easy to be decomposed and utilized by oral bacteria; therefore D-tagatose has low energy , lowering blood sugar, preventing obesity, improving intestinal flora and anti-caries and other physiological functions. In addition to being used as a sweetener, D-tagatose can also be used as a flavor enhancer for food. At present, it is widely used in products such as health drinks, yogurt, fruit juice, baked goods, candies and pharmaceutical preparations (such as syrups, chewable tablets), as a substitute for sucrose.

发明内容 Contents of the invention

本发明要解决的第一个技术问题是一种玉米须矿泉水,该玉米须矿泉水仅使用了玉米须和矿泉水作为原料,不添加任何其它成分。该玉米须矿泉水具有抗氧化、利尿、降压、降血糖和增强机体免疫力等保健功能,不仅可代替饮用水补充人体每日所需要的水分,而且可作为糖尿病人的功能饮品,起到预防保健的功效。The first technical problem to be solved by the present invention is a corn silk mineral water, which only uses corn silk and mineral water as raw materials without adding any other ingredients. The corn silk mineral water has health functions such as anti-oxidation, diuresis, lowering blood pressure, lowering blood sugar and enhancing the body's immunity. The efficacy of preventive health care.

为解决上述技术问题,本发明采用的技术方案为:In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:

本发明提供了一种玉米须矿泉水,它是由玉米须和矿泉水作为原料制成,所述玉米须与矿泉水的重量体积比为0.1~1Kg∶100L;The invention provides a corn silk mineral water, which is made of corn silk and mineral water as raw materials, and the weight-to-volume ratio of the corn silk to the mineral water is 0.1 to 1Kg: 100L;

其中,所述玉米须矿泉水中多糖的含量为0.01~0.5g/L,总皂苷的含量为0.01~0.1g/L,总黄酮的含量为0.01~0.1g/L。Wherein, the polysaccharide content in the corn silk mineral water is 0.01-0.5 g/L, the total saponin content is 0.01-0.1 g/L, and the total flavonoid content is 0.01-0.1 g/L.

本发明还提供了上述玉米须矿泉水的制备方法,该制备方法包括如下步骤:The present invention also provides the preparation method of above-mentioned corn silk mineral water, and this preparation method comprises the steps:

(1)按照玉米须与矿泉水的重量体积比为0.1~1Kg∶100L,称取玉米须和矿泉水,然后将玉米须放入密闭容器中;(1) according to the weight volume ratio of corn silk and mineral water is 0.1~1Kg: 100L, take corn silk and mineral water, then put corn silk into airtight container;

(2)将矿泉水预热,然后在85~95℃的条件下使用预热的矿泉水对玉米须进行热抽提,再进行固液分离,得到滤液A;(2) Preheating the mineral water, and then using the preheated mineral water to perform thermal extraction on corn silk under the condition of 85-95° C., and then performing solid-liquid separation to obtain filtrate A;

(3)将抽提得到的滤液A经过二级过滤,得到玉米须水浸提液;(3) The filtrate A obtained by the extraction is subjected to secondary filtration to obtain the water extract of corn silk;

(4)对玉米须水浸提液进行检测,其中多糖的含量为0.01~0.5g/L,总皂苷的含量为0.01~0.1g/L,总黄酮的含量为0.01~0.1g/L;(4) Detecting the water extract of corn silk, wherein the content of polysaccharides is 0.01~0.5g/L, the content of total saponins is 0.01~0.1g/L, and the content of total flavonoids is 0.01~0.1g/L;

(5)高温瞬时灭菌,冷却至90℃进行热灌装,倒瓶、喷淋冷却,灯检,包装,即为玉米须矿泉水。(5) Instantaneous sterilization at high temperature, cooling to 90°C for hot filling, bottle inversion, spray cooling, light inspection, packaging, and corn silk mineral water.

在所述步骤(2)中,为了达到更好的抽提效果,优选将所述预热的矿泉水分成2~3份分别对玉米须进行热抽提,每次热抽提时间为30min,合并每次获得的滤液,即得滤液A。所述热抽提的温度优选为90℃。In the step (2), in order to achieve a better extraction effect, it is preferable to divide the preheated mineral water into 2 to 3 parts to carry out heat extraction on the corn silk respectively, and each heat extraction time is 30min, Combine the filtrates obtained each time to obtain filtrate A. The temperature of the thermal extraction is preferably 90°C.

本发明要解决的第二个技术问题是一种玉米须矿泉水,该玉米须矿泉水除使用了玉米须和矿泉水作为原料,还添加了适量的D-塔格糖,使得该玉米须矿泉水除具有清香的天然玉米风味之外,口感微甜。The second technical problem to be solved by the present invention is a kind of corn silk mineral water. In addition to using corn silk and mineral water as raw materials, the corn silk mineral water also adds an appropriate amount of D-tagatose, so that the corn silk mineral water In addition to the natural corn flavor of the fragrance, the water tastes slightly sweet.

为解决上述技术问题,本发明采用的技术方案为:In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:

本发明提供了一种玉米须矿泉水,它是由玉米须、D-塔格糖和矿泉水作为原料制成,所述各原料的重量体积比为:玉米须∶D-塔格糖∶矿泉水=0.1~1Kg∶0.01~2Kg∶100L;The invention provides a kind of corn silk mineral water, and it is made by corn silk, D-tagatose and mineral water as raw material, and the weight volume ratio of described each raw material is: corn silk: D-tagatose: mineral spring Water = 0.1~1Kg: 0.01~2Kg: 100L;

其中,所述玉米须矿泉水中多糖的含量为0.01~0.5g/L,总皂苷的含量为0.01~0.1g/L,总黄酮的含量为0.01~0.1g/L。Wherein, the polysaccharide content in the corn silk mineral water is 0.01-0.5 g/L, the total saponin content is 0.01-0.1 g/L, and the total flavonoid content is 0.01-0.1 g/L.

本发明还提供一种上述玉米须矿泉水的制备方法,该制备方法包括如下步骤:The present invention also provides a kind of preparation method of above-mentioned corn silk mineral water, and this preparation method comprises the following steps:

(1)按照玉米须∶D-塔格糖∶矿泉水=0.1~1Kg∶0.01~2Kg∶100L的比例,称取玉米须、D-塔格糖和矿泉水,然后将玉米须放入密闭容器中;(1) According to the ratio of corn silk: D-tagatose: mineral water = 0.1 ~ 1Kg: 0.01 ~ 2Kg: 100L, weigh corn silk, D-tagatose and mineral water, and then put the corn silk into an airtight container middle;

(2)将矿泉水预热,然后在85~95℃的条件下使用预热的矿泉水对玉米须进行热抽提,再进行固液分离,得到滤液A;(2) Preheating the mineral water, and then using the preheated mineral water to perform thermal extraction on corn silk under the condition of 85-95° C., and then performing solid-liquid separation to obtain filtrate A;

(3)将抽提得到的滤液A经过二级过滤,得到玉米须水浸提液;(3) The filtrate A obtained by the extraction is subjected to secondary filtration to obtain the water extract of corn silk;

(4)向玉米须水浸提液中添加D-塔格糖,然后对玉米须水浸提液进行检测,其中多糖的含量为0.01~0.5g/L,总皂苷的含量为0.01~0.1g/L,总黄酮的含量为0.01~0.1g/L;(4) Add D-tagatose to the water extract of corn silk, and then detect the water extract of corn silk, wherein the content of polysaccharide is 0.01~0.5g/L, and the content of total saponin is 0.01~0.1g /L, the content of total flavonoids is 0.01~0.1g/L;

(5)高温瞬时灭菌,冷却至90℃进行热灌装,倒瓶、喷淋冷却,灯检,包装,即为玉米须矿泉水。(5) Instantaneous sterilization at high temperature, cooling to 90°C for hot filling, bottle inversion, spray cooling, light inspection, packaging, and corn silk mineral water.

在所述步骤(2)中,为了达到更好的抽提效果,优选将所述预热的矿泉水分成2~3份分别对玉米须进行热抽提,每次热抽提时间为30min,合并每次获得的滤液,即得滤液A。所述热抽提的温度优选为90℃。In the step (2), in order to achieve a better extraction effect, it is preferable to divide the preheated mineral water into 2 to 3 parts to carry out heat extraction on the corn silk respectively, and each heat extraction time is 30min, Combine the filtrates obtained each time to obtain filtrate A. The temperature of the thermal extraction is preferably 90°C.

上述两种方法中使用的玉米须为新鲜或干燥的玉米须,采收时间在每年的8~10月。The corn silk used in the above two methods is fresh or dry corn silk, and the harvest time is from August to October every year.

本发明的特点和产生的有益效果是:Features of the present invention and the beneficial effects that produce are:

(1)玉米须矿泉水保持天然的备受人们喜爱的玉米风味,清香可口。除此之外,玉米须矿泉水含有适当比例的玉米须多糖、黄酮和皂苷,具有调节免疫功能、抗肿瘤、保肝、降糖、利尿、解热、利胆和降血脂等作用。同时玉米须矿泉水无毒副作用,可作为日常饮品,适合长期饮用,具有维护和修复机体健康的功能。不仅适合于健康人群饮用,也适合于患有糖尿病、高血压等人群的饮用。(1) Corn silk mineral water maintains the natural and popular corn flavor, which is fragrant and delicious. In addition, corn silk mineral water contains a proper proportion of corn silk polysaccharides, flavonoids and saponins, which have the functions of regulating immune function, anti-tumor, protecting liver, lowering blood sugar, diuresis, antipyretic, choleretic and blood lipid lowering. At the same time, corn silk mineral water has no toxic and side effects, can be used as a daily drink, is suitable for long-term drinking, and has the function of maintaining and repairing the health of the body. It is not only suitable for drinking by healthy people, but also suitable for drinking by people with diabetes and high blood pressure.

(2)本发明所述玉米须是在每年8-10月份采收的新鲜或干燥的玉米须,其绿原酸和总黄酮的含量最高,且含量稳定。并且本发明中使用的玉米须未做剪切或磨碎处理,经矿泉水浸提后的滤液澄清,减少后续过滤设备的压力,易于产业化生产。(2) The corn silk of the present invention is fresh or dried corn silk harvested from August to October each year, and has the highest and stable content of chlorogenic acid and total flavonoids. In addition, the corn silk used in the present invention is not cut or ground, and the filtrate after mineral water extraction is clarified, which reduces the pressure of subsequent filtering equipment and is easy for industrial production.

(3)以本发明所述的玉米须与矿泉水的重量体积比范围制备玉米须矿泉水,可以最大限度地提取玉米须中的水溶性物质,特别是玉米须中的特有的风味物质的浸提效果非常明显,并且在制备过程中,采用在密闭容器中进行热抽提,使得本发明所述方法制备的玉米须矿泉水更具有天然的玉米香味,而且由于不添加或仅添加了适量的D-塔格糖,不添加防腐剂成分,使得玉米的香味特别纯正,而且清爽。(3) prepare corn silk mineral water with the weight-volume ratio scope of corn silk and mineral water of the present invention, can extract the water-soluble matter in corn silk to the greatest extent, especially the immersion of the characteristic local flavor substance in corn silk The extraction effect is very obvious, and in the preparation process, heat extraction is carried out in an airtight container, so that the corn silk mineral water prepared by the method of the present invention has more natural corn fragrance, and because no or only an appropriate amount of D-tagatose, without adding preservative ingredients, makes the aroma of corn particularly pure and refreshing.

(4)发明人对玉米须进行热抽提的温度进行了考察,在85~95℃条件下抽提,玉米须水提液中多糖和总皂苷的含量最高,在100℃条件下抽提,总黄酮的含量最高。然而综合考虑玉米须中有效成分的含量以及工业化生产中热耗及成本,最终确定玉米须水生产中浸提温度为85~95℃。(4) The inventor investigated the temperature of hot extraction of corn silk, extracted under the condition of 85~95°C, the content of polysaccharide and total saponin in the aqueous extract of corn silk was the highest, and extracted under the condition of 100°C, The content of total flavonoids is the highest. However, considering the content of active ingredients in corn silk and the heat consumption and cost in industrial production, the extraction temperature in the production of corn silk water is finally determined to be 85-95 °C.

(5)本发明制备方法,简单易行、成本低廉,适用于工业化生产。(5) The preparation method of the present invention is simple, easy to implement and low in cost, and is suitable for industrial production.

(6)玉米是我国主要的农作物之一,玉米资源极其丰富,但是玉米须的开发利用有限,大量的玉米须被废弃,资源浪费严重。本发明玉米须矿泉水的研制成功,开辟了玉米须应用的新途径,将其变废为宝,必将创造巨大的经济效益和社会效益,(6) Corn is one of the main crops in our country. Corn resources are extremely rich, but the development and utilization of corn silk is limited, a large amount of corn silk is discarded, and resources are seriously wasted. The successful development of the corn silk mineral water of the present invention has opened up a new way for the application of corn silk, and turning waste into treasure will certainly create huge economic and social benefits.

附图说明 Description of drawings

下面结合附图对本发明的具体实施方式作进一步详细的说明Below in conjunction with accompanying drawing, specific embodiment of the present invention is described in further detail

图1抽提温度对玉米须水中多糖、总黄酮和总皂苷含量的影响。Fig. 1 The effect of extraction temperature on the content of polysaccharides, total flavonoids and total saponins in corn silk water.

具体实施方式 Detailed ways

下面结合附图和实施例对本发明进一步说明。The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

实施例1 玉米须矿泉水的制备Embodiment 1 The preparation of corn silk mineral water

(1)称取干燥玉米须5kg,去除杂质,洗净,放入密闭的浸提罐中;(1) Take 5 kg of dry corn silk, remove impurities, wash, and put into a closed extraction tank;

(2)将1000L矿泉水预热,预热后的矿泉水均分成3份,在90℃的条件下对玉米须进行3次热抽提,每次热抽提时间为30min,每次热抽提后都进行固液分离,滤液合并;(2) Preheat 1000L mineral water, divide the preheated mineral water into 3 parts, and carry out 3 times of heat extraction on the corn silk under the condition of 90 ℃, each time of heat extraction is 30min, each time of heat extraction Carry out solid-liquid separation after the extraction, and the filtrate is combined;

(3)抽提得到的液体部分经过二级过滤,得到玉米须水浸提液;(3) The liquid part obtained by extraction is subjected to secondary filtration to obtain corn silk water extract;

(4)对玉米须浸提液进行检测,其中玉米须多糖、总黄酮和总皂苷的含量分别为62.7mg/L、29.2mg/L和73.2mg/L。(4) The corn silk extract was detected, and the contents of corn silk polysaccharides, total flavonoids and total saponins were 62.7mg/L, 29.2mg/L and 73.2mg/L, respectively.

(5)经137℃,4sec高温瞬时灭菌后,冷却至90℃进行热灌装,倒瓶、喷淋冷却,灯检,包装,即为玉米须矿泉水,具有清香的天然玉米风味。(5) After being sterilized at 137°C for 4sec, it is cooled to 90°C for hot filling, bottle inversion, spray cooling, light inspection, and packaging. It is corn silk mineral water with a delicate natural corn flavor.

实施例2 玉米须矿泉水的制备The preparation of embodiment 2 corn silk mineral water

(1)-(3)、(5)同实施例1步骤(1)-(3)、(5);(1)-(3), (5) are with embodiment 1 step (1)-(3), (5);

(4)向玉米须水浸提液中添加D-塔格糖1Kg,然后对玉米须水浸提液进行检测,其中玉米须多糖、总黄酮和总皂苷的含量分别为62.7mg/L、29.2mg/L和73.2mg/L。(4) Add 1Kg of D-tagatose to the corn silk water extract, and then detect the corn silk water extract, wherein the contents of corn silk polysaccharides, total flavonoids and total saponins are respectively 62.7mg/L, 29.2 mg/L and 73.2mg/L.

由此方法制备的玉米须矿泉水处具有具有清香的天然玉米风味之外,口感微甜。The corn silk mineral water prepared by the method has a slightly sweet mouthfeel in addition to the natural corn flavor with delicate fragrance.

实施例3 玉米须矿泉水中多糖、总黄酮和总皂苷的测定Example 3 Determination of polysaccharides, total flavonoids and total saponins in corn silk mineral water

1实验材料1 Experimental materials

1.1原料1.1 Raw material

玉米须于2009年8-10月期间在黑龙江省齐齐哈尔市郊区采集的玉米新鲜干燥的花柱和柱头。The fresh and dried styles and stigmas of corn silk collected in the suburbs of Qiqihar City, Heilongjiang Province during August-October 2009.

1.2试剂1.2 Reagents

人参皂苷Re购于中国药品生物制品检定所(110754-200822);芦丁购于北京化学试剂公司;β-葡聚糖是Sigma公司产品。香草醛、浓硫酸、苯酚、乙醇、亚硝酸钠、硝酸铝、氢氧化钠均为市售分析纯试剂。Ginsenoside Re was purchased from China Institute for the Control of Pharmaceutical and Biological Products (110754-200822); rutin was purchased from Beijing Chemical Reagent Company; β-glucan was a product of Sigma. Vanillin, concentrated sulfuric acid, phenol, ethanol, sodium nitrite, aluminum nitrate, and sodium hydroxide are commercially available analytical reagents.

1.3仪器1.3 Instruments

FA1604N电子分析天平                上海精密科学仪器有限公司FA1604N Electronic Analytical Balance Shanghai Precision Scientific Instrument Co., Ltd.

SSW-600-2S电热恒温水槽             上海博讯实业有限公司SSW-600-2S electric heating constant temperature water tank Shanghai Boxun Industrial Co., Ltd.

722可见分光光度计                  上海精密科学仪器有限公司722 Visible Spectrophotometer Shanghai Precision Scientific Instrument Co., Ltd.

Z36HK                              大容量高速冷冻离心机Z36HK Large-capacity high-speed refrigerated centrifuge

2实验方法2 Experimental methods

2.1玉米须水的制备,按照实施例1的方法获得。2.1 The preparation of corn silk water was obtained according to the method in Example 1.

2.2测定方法:2.2 Determination method:

多糖采用硫酸-酚法;总皂苷采用硝酸铝显色法;总黄酮采用香草醛-硫酸比色法。The sulfuric acid-phenol method was used for polysaccharides; the aluminum nitrate colorimetric method was used for total saponins; the vanillin-sulfuric acid colorimetric method was used for total flavonoids.

2.3标准曲线的绘制2.3 Drawing of standard curve

精密称取人参皂苷Re对照品适量,用甲醇溶解,制成0.1812mg/L的对照品母液,精密量取人参皂苷Re对照品母液配制成系列浓度的溶液。分别精密量取上述系列溶液2mL,置于25mL具塞比色管中,精密加入8%香草醛乙醇溶液0.5mL,77%硫酸5mL,摇匀,置60℃水浴中保温10min,取出置冰水浴中冷却10min,以试剂空白液做参比,在534nm波长处测吸光度。以吸光度为纵坐标(Y),以人参皂苷Re的浓度为横坐标(X)绘制标准曲线。Accurately weigh an appropriate amount of ginsenoside Re reference substance, dissolve it with methanol to prepare a 0.1812 mg/L reference substance mother solution, and precisely measure ginsenoside Re reference substance mother solution to prepare solutions with a series of concentrations. Accurately measure 2mL of the above series of solutions, place them in a 25mL stoppered colorimetric tube, precisely add 0.5mL of 8% vanillin ethanol solution, 5mL of 77% sulfuric acid, shake well, put them in a water bath at 60°C for 10 minutes, take them out and put them in an ice water bath Cool in medium for 10 minutes, and measure the absorbance at a wavelength of 534nm with the reagent blank solution as a reference. Draw a standard curve with the absorbance as the ordinate (Y) and the concentration of ginsenoside Re as the abscissa (X).

精密称取芦丁对照品适量,用70%乙醇溶解,制成0.109mg/mL的对照品母液,精密量取芦丁对照品母液配制成系列浓度的溶液。分别精密量取上述系列溶液2mL,置于25mL具塞比色管中,加入5%NaNO2溶液0.3mL,摇匀,放置6min,加入10%Al(NO3)3溶液0.3mL,摇匀,再放置6min,加入4%NaOH 4mL,加蒸馏水稀释至10mL,摇匀,放置10min,以试剂空白液做参比,于506nm处测定吸光度。以吸光度为纵坐标(Y),以芦丁的浓度为横坐标(X)绘制标准曲线。Accurately weigh an appropriate amount of rutin reference substance, dissolve it with 70% ethanol to prepare a 0.109 mg/mL reference substance mother solution, and precisely measure the rutin reference substance mother solution to prepare solutions with a series of concentrations. Accurately measure 2 mL of the above series of solutions respectively, put them in a 25 mL stoppered colorimetric tube, add 0.3 mL of 5% NaNO 2 solution, shake well, let stand for 6 minutes, add 0.3 mL of 10% Al(NO 3 ) 3 solution, shake well, Let stand for 6 minutes, add 4% NaOH 4mL, add distilled water to dilute to 10mL, shake well, let stand for 10min, use reagent blank as reference, measure absorbance at 506nm. Draw a standard curve with the absorbance as the ordinate (Y) and the concentration of rutin as the abscissa (X).

精密称取β-葡聚糖对照品适量,用蒸馏水溶解,制成106mg/L的对照品母液。分别精密量取β-葡聚糖对照品母液0.1mL、0.4mL、0.6mL、0.8mL、1.0mL、1.2mL和1.4mL,置于25mL具塞比色管中,加入蒸馏水至2.0mL,加入6%苯酚溶液1.0mL,摇匀,小心加入浓硫酸5.0mL,摇匀,置沸水浴中煮沸15min,快速冷却后,以试剂空白溶液为参比,在490nm处测定吸光度,以吸光度为纵坐标(Y),以β-葡聚糖的浓度为横坐标(X)绘制标准曲线。Accurately weigh an appropriate amount of β-glucan reference substance, dissolve it in distilled water, and prepare a 106 mg/L reference substance mother solution. Accurately measure 0.1mL, 0.4mL, 0.6mL, 0.8mL, 1.0mL, 1.2mL and 1.4mL of β-glucan reference substance mother solution respectively, put them in a 25mL stoppered colorimetric tube, add distilled water to 2.0mL, add 1.0mL of 6% phenol solution, shake well, carefully add 5.0mL of concentrated sulfuric acid, shake well, put in a boiling water bath and boil for 15min, after rapid cooling, measure the absorbance at 490nm with the reagent blank solution as a reference, take the absorbance as the ordinate (Y), draw a standard curve with the concentration of β-glucan as the abscissa (X).

2.3实验结果2.3 Experimental results

结果表明,标准品人参皂苷Re在18.1~108.7mg/L浓度范围内线性关系良好,回归方程为Y=6.5948X-0.0049,r=0.9985;芦丁在10.9~65.4μg/mL浓度范围内线性关系良好,回归方程为Y=0.0023X-0.0011,r=0.9998;β-葡聚糖在10.6~74.2mg/L浓度范围内线性关系良好,回归方程为Y=9.7686X-0.0191,r=0.9969;The results show that the standard product ginsenoside Re has a good linear relationship in the concentration range of 18.1-108.7 mg/L, and the regression equation is Y=6.5948X-0.0049, r=0.9985; rutin has a linear relationship in the concentration range of 10.9-65.4 μg/mL Good, the regression equation is Y=0.0023X-0.0011, r=0.9998; β-glucan has a good linear relationship within the concentration range of 10.6-74.2mg/L, the regression equation is Y=9.7686X-0.0191, r=0.9969;

精密量取实施例1制备的玉米须矿泉水,采用绘制标准曲线的相关方法和条件,分别测定玉米须水中多糖、总黄酮和总皂苷的含量,即多糖为62.7mg/L,总黄酮为29.2mg/L,总皂苷为73.2mg/L。Precisely measure the corn silk mineral water prepared in Example 1, adopt the relevant methods and conditions for drawing a standard curve, and measure the contents of polysaccharides, total flavonoids and total saponins in the corn silk water respectively, that is, the polysaccharides are 62.7 mg/L, and the total flavones are 29.2 mg/L. mg/L, the total saponin is 73.2mg/L.

实施例4 热抽提温度对玉米须矿泉水中多糖、总黄酮和总皂苷含量的影响Example 4 Effect of heat extraction temperature on polysaccharide, total flavonoids and total saponins content in corn silk mineral water

1实验材料1 Experimental materials

同实施例3。With embodiment 3.

2实验方法2 Experimental methods

按照实施例1的方法,分别在80℃、85℃、90℃、95℃和100℃的条件下进行热抽提,获得不同温度条件下的玉米须浸提液,考察浸提温度对玉米须水中多糖、总黄酮和总皂苷的含量的影响。According to the method of Example 1, heat extraction was carried out under the conditions of 80°C, 85°C, 90°C, 95°C and 100°C respectively, to obtain corn silk extracts under different temperature conditions, and to investigate the effect of extraction temperature on corn silk Effect of polysaccharides, total flavonoids and total saponins in water.

3实验结果3 Experimental results

分别在80℃、85℃、90℃、95℃和100℃的条件下进行制备的玉米须水的多糖、总黄酮和总皂苷含量。如图1所示,在85~95℃条件下浸提,玉米须浸提液中多糖和总皂苷的含量最高,在100℃条件下浸提,总黄酮的含量最高。因此,综合考虑玉米须中有效成分的含量以及工业化生产中热耗及成本,最终确定玉米须矿泉水生产中抽提温度为85~95℃。具体结构见图1。The content of polysaccharides, total flavonoids and total saponins in corn silk water prepared at 80°C, 85°C, 90°C, 95°C and 100°C respectively. As shown in Figure 1, the content of polysaccharides and total saponins in the corn silk extract is the highest when extracted at 85-95 °C, and the highest content of total flavonoids is extracted at 100 °C. Therefore, considering the content of active ingredients in corn silk and the heat consumption and cost in industrial production, the extraction temperature in the production of corn silk mineral water is finally determined to be 85-95 °C. The specific structure is shown in Figure 1.

实施例5 不同玉米须用料量对玉米须水感官的影响Example 5 Effects of Different Corn Silk Consumption on Sensory Effects of Corn Silk Water

1实验材料1 Experimental materials

同实施例3。With embodiment 3.

2实验方法2 Experimental methods

按照实施例1的方法,玉米须的用料量分别为0.1%(w/v)、0.5%(w/v)和1%(w/v),在抽提温度为90℃的条件下制备玉米须水,进行感官指标评价。According to the method of Example 1, the material consumption of corn silk is respectively 0.1% (w/v), 0.5% (w/v) and 1% (w/v), and it is prepared under the condition of 90 ℃ of extraction temperature Corn silk water, for sensory index evaluation.

10名评定员采用不记名打分的方式进行,对颜色、外观、气味、风味、甜味、口腔延迟感觉、生理上的延迟感觉等10项指标进行打分,满分为10分,分数越高,表示效果越好,10 evaluators use anonymous scoring methods to score 10 indicators such as color, appearance, smell, flavor, sweetness, oral delay sensation, and physiological delay sensation. The full score is 10 points, and the higher the score, the better the better the effect,

3实验结果3 Experimental results

根据下列统计结果可知,玉米须用量在0.1~1%之间,玉米须矿泉水的感官评价分值较高,其中玉米须用量为0.5%的玉米须矿泉水的感官评价结果最好,为545分。主要区别在于色泽和口感方面,玉米须用量0.5%的玉米须矿泉水清澈透明,略有黄色,具有天然的玉米清香风味。而玉米须用量为0.01%和0.05%的玉米须矿泉水过于清淡,相反玉米须用量在1%以上的玉米须矿泉水又过于浓重。综合下列指标的综合分数,0.5%浓度的玉米须矿泉水的感官指标最好。According to the following statistical results, it can be seen that the sensory evaluation score of corn silk mineral water is higher when the amount of corn silk is between 0.1% and 1%, and the sensory evaluation score of corn silk mineral water is the best, which is 545. point. The main difference lies in the color and taste. Corn silk mineral water with 0.5% corn silk is clear and transparent, slightly yellow, and has a natural fragrance of corn. And the corn silk mineral water that the corn silk consumption is 0.01% and 0.05% is too light, on the contrary the corn silk mineral water that the corn silk consumption is more than 1% is too dense again. Based on the comprehensive scores of the following indicators, the sensory indicators of corn silk mineral water with a concentration of 0.5% are the best.

结果如表1所示:The results are shown in Table 1:

显然,本发明的上述实施例仅仅是为清楚地说明本发明所作的举例,而并非是对本发明的实施方式的限定。对于所属领域的普通技术人员来说,在上述说明的基础上还可以做出其它不同形式的变化或变动。这里无法对所有的实施方式予以穷举。凡是属于本发明的技术方案所引伸出的显而易见的变化或变动仍处于本发明的保护范围之列。Apparently, the above-mentioned embodiments of the present invention are only examples for clearly illustrating the present invention, rather than limiting the implementation of the present invention. For those of ordinary skill in the art, on the basis of the above description, other changes or changes in different forms can also be made. All the implementation manners cannot be exhaustively listed here. All obvious changes or changes derived from the technical solutions of the present invention are still within the protection scope of the present invention.

Claims (8)

1. a corn stigma mineral water is characterized in that, it is to be processed as raw material by corn stigma and mineral water, and the w/v of said corn stigma and mineral water is 0.1~1Kg: 100L;
Wherein, the content of polysaccharide is 0.01~0.5g/L in the said corn stigma mineral water, and the content of total saponin(e is 0.01~0.1g/L, and content of total flavone is 0.01~0.1g/L;
Concrete preparation method comprises the steps:
(1) takes by weighing corn stigma and mineral water according to aforementioned proportion, then corn stigma is put into closed container;
(2) with the mineral water preheating, under 85~95 ℃ condition, use the mineral water of preheating that corn stigma is carried out hot extracting then, carry out Separation of Solid and Liquid again, obtain the A that filtrates;
(3) the filtrating A process cascade filtration that extracting is obtained obtains the corn stigma water extract;
(4) the corn stigma water extract is detected, wherein the content of polysaccharide is 0.01~0.5g/L, and the content of total saponin(e is 0.01~0.1g/L, and content of total flavone is 0.01~0.1g/L;
(5) high-temperature short-time sterilization is cooled to 90 ℃ and carries out hot filling, bottle, spray cooling, and the lamp inspection is packed, and is the corn stigma mineral water.
2. the preparation method of corn stigma mineral water as claimed in claim 1 is characterized in that, this preparation method comprises the steps:
(1) takes by weighing corn stigma and mineral water according to aforementioned proportion, then corn stigma is put into closed container;
(2) with the mineral water preheating, under 85~95 ℃ condition, use the mineral water of preheating that corn stigma is carried out hot extracting then, carry out Separation of Solid and Liquid again, obtain the A that filtrates;
(3) the filtrating A process cascade filtration that extracting is obtained obtains the corn stigma water extract;
(4) the corn stigma water extract is detected, wherein the content of polysaccharide is 0.01~0.5g/L, and the content of total saponin(e is 0.01~0.1g/L, and content of total flavone is 0.01~0.1g/L;
(5) high-temperature short-time sterilization is cooled to 90 ℃ and carries out hot filling, bottle, spray cooling, and the lamp inspection is packed, and is the corn stigma mineral water.
3. preparation method according to claim 2 is characterized in that, in said step (2); The mineral water of said preheating is divided into 2~3 parts respectively corn stigma is carried out hot extracting; Each hot extracting time is 30min, merges each filtrating that obtains, and promptly gets the A that filtrates.
4. according to claim 2 or 3 described preparation methods, it is characterized in that, in the said step (2), under 90 ℃ condition, use the mineral water of preheating that corn stigma is carried out hot extracting.
5. a corn stigma mineral water is characterized in that, it is to be processed as raw material by corn stigma, D-Tagatose and mineral water, and the w/v of said each raw material is: corn stigma: the D-Tagatose: mineral water=0.1~1Kg: 0.01~2Kg: 100L;
Wherein, the content of polysaccharide is 0.01~0.5g/L in the said corn stigma mineral water, and the content of total saponin(e is 0.01~0.1g/L, and content of total flavone is 0.01~0.1g/L;
Concrete preparation method comprises the steps:
(1) takes by weighing corn stigma, D-Tagatose and mineral water according to aforementioned proportion, then corn stigma is put into closed container;
(2) with the mineral water preheating, under 85~95 ℃ condition, use the mineral water of preheating that corn stigma is carried out hot extracting then, carry out Separation of Solid and Liquid again, obtain the A that filtrates;
(3) the filtrating A process cascade filtration that extracting is obtained obtains the corn stigma water extract;
(4) in the corn stigma water extract, add the D-Tagatose, then the corn stigma water extract is detected, wherein the content of polysaccharide is 0.01~0.5g/L, and the content of total saponin(e is 0.01~0.1g/L, and content of total flavone is 0.01~0.1g/L;
(5) high-temperature short-time sterilization is cooled to 90 ℃ and carries out hot filling, bottle, spray cooling, and the lamp inspection is packed, and is the corn stigma mineral water.
6. the preparation method of corn stigma mineral water as claimed in claim 5 is characterized in that, this preparation method comprises the steps:
(1) takes by weighing corn stigma, D-Tagatose and mineral water according to aforementioned proportion, then corn stigma is put into closed container;
(2) with the mineral water preheating, under 85~95 ℃ condition, use the mineral water of preheating that corn stigma is carried out hot extracting then, carry out Separation of Solid and Liquid again, obtain the A that filtrates;
(3) the filtrating A process cascade filtration that extracting is obtained obtains the corn stigma water extract;
(4) in the corn stigma water extract, add the D-Tagatose, then the corn stigma water extract is detected, wherein the content of polysaccharide is 0.01~0.5g/L, and the content of total saponin(e is 0.01~0.1g/L, and content of total flavone is 0.01~0.1g/L;
(5) high-temperature short-time sterilization is cooled to 90 ℃ and carries out hot filling, bottle, spray cooling, and the lamp inspection is packed, and is the corn stigma mineral water.
7. preparation method according to claim 6 is characterized in that, in said step (2), the mineral water of preheating is divided into 2~3 parts respectively corn stigma is carried out hot extracting, and each hot extracting time is 30min, merges each filtrating that obtains, and promptly gets the A that filtrates.
8. according to claim 6 or 7 described preparation methods, it is characterized in that, in the said step (2), under 90 ℃ condition, use the mineral water of preheating that corn stigma is carried out hot extracting.
CN201010256249A 2010-08-18 2010-08-18 A kind of corn silk mineral water and preparation method thereof Expired - Fee Related CN101940349B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201010256249A CN101940349B (en) 2010-08-18 2010-08-18 A kind of corn silk mineral water and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201010256249A CN101940349B (en) 2010-08-18 2010-08-18 A kind of corn silk mineral water and preparation method thereof

Publications (2)

Publication Number Publication Date
CN101940349A CN101940349A (en) 2011-01-12
CN101940349B true CN101940349B (en) 2012-10-17

Family

ID=43432706

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201010256249A Expired - Fee Related CN101940349B (en) 2010-08-18 2010-08-18 A kind of corn silk mineral water and preparation method thereof

Country Status (1)

Country Link
CN (1) CN101940349B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102485261B (en) * 2010-12-03 2014-10-29 吉林吉春制药股份有限公司 Medicine for treating diabetes and preparation method thereof
CN103478845B (en) * 2013-09-06 2016-04-06 哈尔滨玉农源食品有限公司 A kind of corn stigma extraction concentrate and preparation method thereof
CN105125941A (en) * 2015-09-30 2015-12-09 华心愉 Preparation method of assistant hypoglycemic corn-stigma extractive capsules
CN106336060A (en) * 2016-10-20 2017-01-18 绥江县绥源食品有限责任公司 Mountain spring water production process
CN108576522A (en) * 2018-01-06 2018-09-28 佛山市三水区嘉华化学研究院(普通合伙) A kind of Pinctada martensii beverage and preparation method thereof
CN111493244A (en) * 2020-03-29 2020-08-07 湖南省拜尔艾克斯生物技术有限公司 Edible plant blood sugar reducing beverage and preparation method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101796997A (en) * 2009-02-05 2010-08-11 魏庆梅 Corn stigma tea

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101796997A (en) * 2009-02-05 2010-08-11 魏庆梅 Corn stigma tea

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
温宪春.玉米须水提物对2型糖尿病大鼠糖_脂代谢及氧化应激的影响.《中国中医药信息杂志》.2010,第17卷(第5期),第24-26页.
温宪春.玉米须水提物对2型糖尿病大鼠胰高血糖素的影响.《医学研究杂志》.2010,第39卷(第4期),第52-54页.
玉米须水提物对2型糖尿病大鼠糖_脂代谢及氧化应激的影响;温宪春;《中国中医药信息杂志》;20100531;第17卷(第5期);第24-26页 *
玉米须水提物对2型糖尿病大鼠胰高血糖素的影响;温宪春;《医学研究杂志》;20100430;第39卷(第4期);第52-54页 *

Also Published As

Publication number Publication date
CN101940349A (en) 2011-01-12

Similar Documents

Publication Publication Date Title
CN101946944A (en) Corn silk water and preparation method thereof
CN101940349B (en) A kind of corn silk mineral water and preparation method thereof
CN103169129B (en) A kind of preparation method and its usage of corn silk polysaccharide functional drinks
CN103168883A (en) Pathogenic microorganism preventing antiviral tea beverage granules capable of boosting immunity and preparation method thereof
CN106962934A (en) A kind of alcohol-decomposing beverage based on Tea Saponin
CN105029605A (en) Preparation method of jasmine flower health ferment beverage
CN108783155A (en) A kind of sealwort, mulberry leaf, radix polygonati officinalis compatibility beverage and preparation method thereof
CN102697129B (en) Semen hoveniae plant beverage and preparation method thereof
CN101433360B (en) a lotus leaf jelly
KR20090040810A (en) Liver protective composition containing fentorinarin or fentorriginin
CN111919982A (en) Natural plant beverage for relieving alcoholism and preparation method thereof
CN115474640A (en) A kind of functional wood ginger leaf tea drink and preparation method thereof
JP2006262889A (en) A composition for food containing Kuzuka and a specific food material
CN104522244A (en) Sugar-free momordica grosvenori drink and production method thereof
CN103520287B (en) Forsythia suspense leaves refreshing sugar
CN111802542A (en) Cranberry plant beverage containing prebiotics and preparation method thereof
KR102173223B1 (en) Food composition containing extract of roasted codonopsis lanceolata or roasted platycodon grandiflorum
CN106490410A (en) A kind of coconut palm Water-Electrolyte fluid infusion compositionss and preparation method thereof
CN104738775A (en) Plant beverage and preparation technology thereof
US20100297268A1 (en) Agent for increasing blood adiponectin quantity
CN108719972A (en) A kind of composite enzyme and preparation method thereof with anti-alcohol function
CN110521895A (en) A kind of compound hypoglycemic beverage of moringa tangerine peel and preparation method thereof
CN113303383A (en) Lithocarpus polystachyus rehd black tea compound beverage and preparation method thereof
KR100642801B1 (en) Antidiabetic Food Composition Combining Herbal Medicines and Pear Extracts and Manufacturing Method Thereof
CN110353137A (en) A kind of preparation method of water chestnut stem juice compound beverage

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
ASS Succession or assignment of patent right

Owner name: HEILONGJIANG TONGXIN INTEGRITY TECHNOLOGY DEVELOPM

Free format text: FORMER OWNER: QIQIHAR MEDICAL COLLEGE

Effective date: 20131107

C41 Transfer of patent application or patent right or utility model
COR Change of bibliographic data

Free format text: CORRECT: ADDRESS; FROM: 161006 QIQIHAR, HEILONGJIANG PROVINCE TO: 151000 HARBIN, HEILONGJIANG PROVINCE

TR01 Transfer of patent right

Effective date of registration: 20131107

Address after: 151000 No. 85, Nantong Avenue, Nangang District, Heilongjiang, Harbin

Patentee after: Heilongjiang Tongxin integrity Technology Development Co.,Ltd.

Address before: Bu Kui 161006 North Main Street in Qigihar City, Heilongjiang province Jianhua District No. 333

Patentee before: Qiqihar Medical University

TR01 Transfer of patent right

Effective date of registration: 20211222

Address after: 150001 Room 403, unit 1, building 15, Xuanqing community, Nangang District, Harbin City, Heilongjiang Province

Patentee after: Zhang Guojun

Address before: 151000 No. 85, Nantong street, Nangang District, Harbin City, Heilongjiang Province

Patentee before: Heilongjiang Tongxin integrity Technology Development Co.,Ltd.

TR01 Transfer of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20121017

CF01 Termination of patent right due to non-payment of annual fee