CN101912041B - Preparation method of active rhodotorula glutinis powder - Google Patents

Preparation method of active rhodotorula glutinis powder Download PDF

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Publication number
CN101912041B
CN101912041B CN2010102421204A CN201010242120A CN101912041B CN 101912041 B CN101912041 B CN 101912041B CN 2010102421204 A CN2010102421204 A CN 2010102421204A CN 201010242120 A CN201010242120 A CN 201010242120A CN 101912041 B CN101912041 B CN 101912041B
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percent
weight
rhodotorula glutinis
hours
drying
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CN2010102421204A
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Chinese (zh)
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CN101912041A (en
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李晓梅
陈成
姜帆
滕春红
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哈尔滨市海澳斯生物科技开发有限公司
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Abstract

The invention provides a preparation method of active rhodotorula glutinis powder, comprising the following steps of: firstly preparing directly throwing type leaven freeze-drying bacterium powder by taking rhodotorula glutinis as a parent strain, and inoculating 150 g of the directly throwing type leaven on every ton of fermentation mediums at 28-30 DEG C, wherein ventilation quantity is 80-95 m<3>/hour; after 24 hours, adding a nutrient source prepared from 2 percent by weight of cane sugar, 0.3 percent by weight of urea and 0.1 percent by weight of ammonium sulfate in continuous flow for 18 hours, and culturing for 48-55 hours; then taking a yeast concentrated solution by adopting a tube centrifuge for 5000 turns/minute, and adding 0.1 percent by weight of sucrose esters, 0.05 percent by weight of glycerin monostearate, 0.1 percent by weight of xylo-oligosaccharides and 1.5-5 percent by weight of dextrin according to the weight of yeast concentrated solution, sufficiently dissolving the added substances into the yeast concentrated solution, and uniformly stirring; ventilating and drying at 50 DEG C to ensure that the moisture of the additive substances is 20-25 percent, and pelleting; then continuously drying at 50 DEG C to ensure that the moisture of particles is lower than 5 percent; and then carrying out vacuum packaging.

Description

A kind of preparation method of active rhodotorula glutinis powder

(1) technical field

The present invention relates to a kind of preparation method of active rhodotorula glutinis powder, be specifically related to a kind of quick method that adopts throw type leaven, produce active rhodotorula glutinis powder, belong to bioengineering field.

(2) background technology

Rhodotorula glutinis contains multiple nutritional components such as rich in amino acid, astaxanthin; Not only in the attention more and more widely of having arrived of aspects such as medicine, food, chemical industry and agricultural, and rhodotorula glutinis is good production of astaxanthin bacterium and the live single-cell ecological bait that has potentiality.Aspect aquaculture, can replace traditional bait such as unit cell algae to be used for the young open-mouthed bait of marine animal and the complementarity bait in whole young period; Strengthen aquatic animal immunizing power, substitute the microbiotic use; Being the good additive of ecologic breeding, is natural, healthy little ecological active bacteria formulation.

Carrotenoid mainly comprises β-Hu Luobusu, astaxanthin; Have the prevention arteriosclerosis, suppress the tumour generation, increase functions such as host immune power and resistance of oxidation; Regarded as category-A nutrition pigment by international organizations such as FAO and WHO, and get permission foodstuff additive as nutrition, painted dual function more than 50 countries and regions.β-Hu Luobusu is widely used in medicines and health protection, foods and cosmetics industry.Since the toxicity problem of synthetic colour, the use of many countries restriction synthetic colour.Utilize rhodotorula glutinis biosynthesizing β-Hu Luobusu to have the following advantages: thalline is nontoxic, and contains rich in protein and VITAMINs; Bagasse capable of using, waste molasses etc. are cultivated, and cost is low and the cycle short, fermentation control is simple, helps suitability for industrialized production, therefore, utilizes rhodotorula glutinis biosynthesizing beta carotene, will become the important source of natural beta-carotin.

The domestic several families enterprise production rhodotorula glutinis few in number and the mode of production of three grades of liquid state fermentations of series product employing or solid state fermentation thereof, it is long to exist fermentation period, and the product activity is low; Complicated operation; Many defectives such as easy bacteria infection, the present invention adopts the form of throw type leaven, has shortened fermentation time greatly; Enhance productivity and improved product purity and biological activity plant factor the time; This working of an invention can bring huge economic benefit and social benefit, has the wide development prospect.

(3) summary of the invention

The object of the invention is to provide a kind of preparation method of active rhodotorula glutinis powder.

The objective of the invention is to realize like this: related per-cent removes other among the present invention has and is mass ratio indicating, and the such method of product employing of the present invention prepares:

1. the preparation of the selection of rhodotorula glutinis and substratum

The rhodotorula glutinis (Rhodotorula glutinis) that the present invention selects is purchased in the Chinese industrial microbial strains and is contained the center, is numbered 32881, and bacterium culture medium is: 5 ° of B é worts.

2. the preparation of throw type leaven

2.1 the preparation of mother starter

Measure Rhodotorula glutinis fungus substratum 200ml in 1 500ml triangular flask, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, with transfering loop inoculation rhodotorula glutinis bacterial strain inclined-plane one ring, cultivated 48 hours at 28-30 ℃, 150 rev/mins shaking tables, as mother starter.

2.2 the preparation of productive leavening agent

At first dispose 20% skimmed milk emulsion, 121 ℃ of sterilizations were cooled to 28-30 ℃ in 20 minutes, and by 5% volume ratio inoculation mother starter, 28-30 ℃, 150 rev/mins shaking tables were cultivated 48 hours, detected skimmed milk emulsion rhodotorula glutinis viable count>=10 9Individual/ml, treat as and be fermenting-ripening, if viable count<10 9Individual/ml, continue to cultivate, until reaching 10 9Individual/ml, finish to cultivate, freeze-drying is for use.

2.3 the preparation of throw type leaven

Under aseptic condition, import sophisticated productive leavening agent in the glass ampoule; Liquid level is lower than 0.8-1cm, puts into-30 ℃ of refrigerator-freezer quick-frozens after adding a cover bottle stopper, after freezing glass ampoule is taken advantage of dress with pallet; Put into Freeze Drying Equipment and carry out lyophilize, make throw type leaven.

3. the preparation of active rhodotorula glutinis powder

3.1 the preparation of fermention medium

Glucose 2-5%

Sucrose 3%

Sal epsom 0.15%

Yeast extract paste (moisture content<30%) 0.2%

Potassium hydrogenphosphate 0.5%

Urea 0.2%

Ammonium sulfate 0.1%

Saltpetre 0.1%

PH value 4-5

3.2 rhodotorula glutinis fermentation culture

Inoculum size: fermention medium inoculation 150g throw type leaven per ton.

Culture condition: 28-30 ℃, ventilation 80-95m 3/ hour, incubation time: 48-55 hour.

The nutrition source and course adds condition: press fermention medium weight and add nutrition source, comprising: sucrose 2%, and urea 0.3%, ammonium sulfate 0.1%, with the solution that is mixed with 30% after three kinds of raw materials mix, even flow Ensure Liquid source after fermenting 24 hours added in 18 hours.

3.3 rhodotorula glutinis concentrates

Adopt tubular-bowl centrifuge, 5000 rev/mins, get the yeast liquid concentrator; Add 0.1% sucrose ester by yeast liquid concentrator weight, 0.05% glyceryl monostearate, 0.1% xylooligosaccharides; The 1.5-5% dextrin fully is dissolved in these substance in the yeast liquid concentrator and stirs.

3.4 rhodotorula glutinis finished product preparation

50 ℃ of air seasonings make its moisture at 20-25%, carry out granulation, and 50 ℃ are continued drying then, make pellet moisture be lower than 5%, carry out vacuum packaging then.

(4) embodiment

Below in conjunction with specific embodiment the present invention is made more detailed description:

Embodiment one:

1. the preparation of the selection of rhodotorula glutinis and substratum

The rhodotorula glutinis (Rhodotorula glutinis) that the present invention selects is purchased in the Chinese industrial microbial strains and is contained the center, is numbered 32881, and bacterium culture medium is: 5 ° of B é worts.

2. the preparation of throw type leaven

2.1 the preparation of mother starter

Measure Rhodotorula glutinis fungus substratum 200ml in 1 500ml triangular flask, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, with transfering loop inoculation rhodotorula glutinis bacterial strain inclined-plane one ring, cultivated 48 hours at 28-30 ℃, 150 rev/mins shaking tables, as mother starter.

2.2 the preparation of productive leavening agent

At first dispose 20% skimmed milk emulsion, 121 ℃ of sterilizations were cooled to 28-30 ℃ in 20 minutes, and by 5% volume ratio inoculation mother starter, 28-30 ℃, 150 rev/mins shaking tables were cultivated 48 hours, detected skimmed milk emulsion rhodotorula glutinis viable count>=10 9Individual/ml, treat as and be fermenting-ripening, if viable count<10 9Individual/ml, continue to cultivate, until reaching 10 9Individual/ml, finish to cultivate, freeze-drying is for use.

2.3 the preparation of throw type leaven

Under aseptic condition, import sophisticated productive leavening agent in the glass ampoule; Liquid level is lower than 0.8-1cm, puts into-30 ℃ of refrigerator-freezer quick-frozens after adding a cover bottle stopper, after freezing glass ampoule is taken advantage of dress with pallet; Put into Freeze Drying Equipment and carry out lyophilize, make throw type leaven.

3. the preparation of active rhodotorula glutinis powder

3.1 the preparation of fermention medium

Glucose 2-5%

Sucrose 3%

Sal epsom 0.15%

Yeast extract paste (moisture content<30%) 0.2%

Potassium hydrogenphosphate 0.5%

Urea 0.2%

Ammonium sulfate 0.1%

Saltpetre 0.1%

PH value 4-5

3.2 rhodotorula glutinis fermentation culture

Inoculum size: fermention medium inoculation 150g throw type leaven per ton.

Culture condition: 28-30 ℃, ventilation 80-95m 3/ hour, incubation time: 48-55 hour.

The nutrition source and course adds condition: press fermention medium weight and add nutrition source, comprising: sucrose 2%, and urea 0.3%, ammonium sulfate 0.1%, with the solution that is mixed with 30% after three kinds of raw materials mix, even flow Ensure Liquid source after fermenting 24 hours added in 18 hours.

3.3 rhodotorula glutinis concentrates

Adopt tubular-bowl centrifuge, 5000 rev/mins, get the yeast liquid concentrator; Add 0.1% sucrose ester by yeast liquid concentrator weight, 0.05% glyceryl monostearate, 0.1% xylooligosaccharides; 1.5% dextrin fully is dissolved in these substance in the yeast liquid concentrator and stirs.

3.4 rhodotorula glutinis finished product preparation

50 ℃ of air seasonings make its moisture at 20-25%, carry out granulation, and 50 ℃ are continued drying then, make pellet moisture be lower than 5%, carry out vacuum packaging then.

Embodiment two:

1. the preparation of the selection of rhodotorula glutinis and substratum

The rhodotorula glutinis (Rhodotorula glutinis) that the present invention selects is purchased in the Chinese industrial microbial strains and is contained the center, is numbered 32881, and bacterium culture medium is: 5 ° of B é worts.

2. the preparation of throw type leaven

2.1 the preparation of mother starter

Measure Rhodotorula glutinis fungus substratum 200ml in 1 500ml triangular flask, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, with transfering loop inoculation rhodotorula glutinis bacterial strain inclined-plane one ring, cultivated 48 hours at 28-30 ℃, 150 rev/mins shaking tables, as mother starter.

2.2 the preparation of productive leavening agent

At first dispose 20% skimmed milk emulsion, 121 ℃ of sterilizations were cooled to 28-30 ℃ in 20 minutes, and by 5% volume ratio inoculation mother starter, 28-30 ℃, 150 rev/mins shaking tables were cultivated 48 hours, detected skimmed milk emulsion rhodotorula glutinis viable count>=10 9Individual/ml, treat as and be fermenting-ripening, if viable count<10 9Individual/ml, continue to cultivate, until reaching 10 9Individual/ml, finish to cultivate, freeze-drying is for use.

2.3 the preparation of throw type leaven

Under aseptic condition, import sophisticated productive leavening agent in the glass ampoule; Liquid level is lower than 0.8-1cm, puts into-30 ℃ of refrigerator-freezer quick-frozens after adding a cover bottle stopper, after freezing glass ampoule is taken advantage of dress with pallet; Put into Freeze Drying Equipment and carry out lyophilize, make throw type leaven.

3. the preparation of active rhodotorula glutinis powder

3.1 the preparation of fermention medium

Glucose 2-5%

Sucrose 3%

Sal epsom 0.15%

Yeast extract paste (moisture content<30%) 0.2%

Potassium hydrogenphosphate 0.5%

Urea 0.2%

Ammonium sulfate 0.1%

Saltpetre 0.1%

PH value 4-5

3.2 rhodotorula glutinis fermentation culture

Inoculum size: fermention medium inoculation 150g throw type leaven per ton.

Culture condition: 28-30 ℃, ventilation 80-95m 3/ hour, incubation time: 48-55 hour.

The nutrition source and course adds condition: press fermention medium weight and add nutrition source, comprising: sucrose 2%, and urea 0.3%, ammonium sulfate 0.1%, with the solution that is mixed with 30% after three kinds of raw materials mix, even flow Ensure Liquid source after fermenting 24 hours added in 18 hours.

3.3 rhodotorula glutinis concentrates

Adopt tubular-bowl centrifuge, 5000 rev/mins, get the yeast liquid concentrator; Add 0.1% sucrose ester by yeast liquid concentrator weight, 0.05% glyceryl monostearate, 0.1% xylooligosaccharides; 5% dextrin fully is dissolved in these substance in the yeast liquid concentrator and stirs.

3.4 rhodotorula glutinis finished product preparation

50 ℃ of air seasonings make its moisture 2025%, carry out granulation, and 50 ℃ are continued drying then, make pellet moisture be lower than 5%, carry out vacuum packaging then.

Claims (1)

1. the preparation method of an active rhodotorula glutinis powder; It is characterized in that: throw type leaven adopts this quadrat method preparation: measure 5 ° of B é wort 200ml in 1 500ml triangular flask; 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes; Purchase with transfering loop inoculation and to be numbered 32881 rhodotorula glutinis bacterial strain inclined-plane one ring in Chinese industrial microbial strains preservation center, cultivated 48 hours at 28-30 ℃, 150 rev/mins shaking tables, as mother starter; Prepare 20% skimmed milk emulsion then, 121 ℃ of sterilizations were cooled to 28-30 ℃ in 20 minutes, and by 5% volume ratio inoculation mother starter, 28-30 ℃, 150 rev/mins shaking tables were cultivated 48 hours, detected skimmed milk emulsion rhodotorula glutinis viable count>=10 9Individual/ml, treat as and be fermenting-ripening, if viable count<10 9Individual/ml, continue to cultivate, until reaching 10 9Individual/ml, finish to cultivate, as sophisticated productive leavening agent, freeze-drying is for use; Sophisticated productive leavening agent is imported in the glass ampoule under aseptic condition, and liquid level is lower than 0.8cm, puts into-30 ℃ of refrigerator-freezer quick-frozens after adding a cover bottle stopper, after freezing glass ampoule is used the pallet splendid attire, puts into Freeze Drying Equipment and carries out lyophilize, makes throw type leaven; Fermention medium adopts this quadrat method preparation: glucose 2-5%, sucrose 3%, sal epsom 0.15%, the yeast extract paste 0.2% of moisture content<30%, potassium hydrogenphosphate 0.5%, urea 0.2%, ammonium sulfate 0.1%, saltpetre 0.1%, pH value 4-5; Fermention medium inoculation 150g throw type leaven per ton, 28-30 ℃, ventilation 80-95m 3/ hour, through overcurrent Ensure Liquid source, incubation time 48-55 hour, adopt tubular-bowl centrifuge then, 5000 rev/mins; Get the yeast liquid concentrator, add 0.1% sucrose ester, 0.05% glyceryl monostearate, 0.1% xylooligosaccharides by yeast liquid concentrator weight; The 1.5-5% dextrin fully is dissolved in these substance in the yeast liquid concentrator and stirs, 50 ℃ of air seasonings, and its moisture is at 20-25%; Carry out granulation, 50 ℃ are continued drying then, and pellet moisture is lower than 5%, carries out vacuum packaging then.
CN2010102421204A 2010-08-02 2010-08-02 Preparation method of active rhodotorula glutinis powder CN101912041B (en)

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CN102119767A (en) * 2011-01-25 2011-07-13 王立新 Production technology of eggs with high carotene content
CN103848651B (en) * 2012-11-30 2016-03-30 北京农学院 A kind of preparation method of rhodotorula glutinis bio-feritlizer and application
CN106690124A (en) * 2016-12-15 2017-05-24 湖北工业大学 Method for producing vinasse flavored striped tuna containing rich beta-carotene by utilizing vinasse

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CN1058989A (en) * 1991-08-28 1992-02-26 中国生物工程开发中心宜昌食用酵母基地 Process for refractory alcohol active dried yeast
CN101173222A (en) * 2007-10-15 2008-05-07 四川循环科技有限公司 Method for producing active dry yeast for sweet potato produced amylolysis ferment
CN101575578B (en) * 2008-05-06 2012-07-11 安琪酵母股份有限公司 Saccharomyces cerevisiae, dry yeast rich in reduced glutathione and preparation method thereof
CN101570735B (en) * 2009-06-12 2011-04-20 河南工业大学 Method for producing flour yeast by using corn flour as raw material

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Inventor after: Li Xiaomei

Inventor after: Chen Cheng

Inventor after: Jiang Fan

Inventor after: Teng Chunhong

Inventor before: Chen Cheng

Inventor before: Teng Chunhong

COR Change of bibliographic data

Free format text: CORRECT: INVENTOR; FROM: CHEN CHENG TENG CHUNHONG TO: LI XIAOMEI CHEN CHENG JIANG FAN TENG CHUNHONG

C14 Grant of patent or utility model
GR01 Patent grant
C41 Transfer of patent application or patent right or utility model
TR01 Transfer of patent right

Effective date of registration: 20160525

Address after: 841000, 58 Korla Road, the Xinjiang Uygur Autonomous Region economic and Technological Development Zone

Patentee after: Xinjiang Kerry Food Technology Co., Ltd.

Address before: 150030 Heilongjiang Province, Harbin city Xiangfang District Gongbin road Wood Street No. 59

Patentee before: Harbin Haiaosi Biotechnology Development Co., Ltd.