CN101875910A - Bacillus amyloliquefaciens for producing gamma-polyglutamic acid - Google Patents
Bacillus amyloliquefaciens for producing gamma-polyglutamic acid Download PDFInfo
- Publication number
- CN101875910A CN101875910A CN 201010150439 CN201010150439A CN101875910A CN 101875910 A CN101875910 A CN 101875910A CN 201010150439 CN201010150439 CN 201010150439 CN 201010150439 A CN201010150439 A CN 201010150439A CN 101875910 A CN101875910 A CN 101875910A
- Authority
- CN
- China
- Prior art keywords
- gamma
- polyglutamic acid
- cgmcc
- hour
- bacterial strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses bacillus for producing gamma-polyglutamic acid, which is named as bacillus amyloliquefaciens SFPG-123 and preserved in CGMCC with a preservation number CGMCC No.3447 on November 16th, 2009. The bacillus provided by the invention can transform glutamic acid, citric acid and the like to generate the gamma-polyglutamic acid; the yield of the gamma-polyglutamic acid in unit volume fermentation liquor can reach 15 to 20g/L; and the bacillus has research and development values.
Description
Technical field
The present invention relates to a bacillus, relate in particular to a strain and can produce gamma-polyglutamic acid-(the bacillus amyloliquefaciens bacterial strain of γ-PGA) belongs to biological technical field.
Background technology
Poly-gamma-glutamic acid (Poly-γ-glutamic acid; γ-PGA) is by the outer aminoacid polymers of a kind of born of the same parents of microorganisms, form by γ-carboxyl and alpha-amino group condensation by L-L-glutamic acid (L-Glu) and D-L-glutamic acid (D-Glu) monomer, normally be made up of about 500～5000 L-glutamic acid monomer, relative molecular mass is between 100～1000kD.In the poly-gamma-glutamic acid molecule a large amount of free wetting ability carboxyls is arranged, can between intramolecule or molecule, form hydrogen bond, have high water-soluble and suction moisture retention, the maximum multiple that absorbs water naturally of γ--PGA can reach 1100 times, is 30～80 times to the absorption multiple of soil moisture.The infusion of γ-PGA has moisture holding capacity preferably and ideal releasing effect in soil, have tangible drought resisting to urge the seedling effect, can be widely used in arid area water conservation and desert afforestation.Poly-gamma-glutamic acid is nontoxic to have excellent biological compatibility and degradability, can provide controlled drug release, target as pharmaceutical carrier, and it is water-soluble to improve medicine, reduce adverse drug reaction, improve curative effect of medication, γ-PGA also can reduce the toxic side effect of medicine, strengthens stability of drug.In addition, γ-PGA also has film-forming properties, becomes the physics and chemistry and the biological characteristics of many uniquenesses such as fibering, plasticity-, cohesiveness, flocculence, and can not pollute environment, be the biomacromolecule of a kind of green, environmental protection, in agricultural, pharmaceutical industries, foodstuffs industry, cosmetic industry and environmental protection, have broad prospect of application.
Poly-gamma-glutamic acid can pass through chemical synthesis, enzyme transforming process and Production by Microorganism Fermentation at present.Chemical synthesis is produced poly-gamma-glutamic acid process complexity, difficulty is big, yield is low, industrial application value is little, but has certain theory and using value for the structure of understanding γ-PGA and the modification technique of function relationship and development γ-PGA practical application etc.Enzyme transforming process is to be monomer with L-glutamic acid; adopt glutamyltranspeptidase (GTP) step enzymatic reaction; the catalysis glutamyl is transferred on the acceptor; peptide when being same substance, donor and acceptor then can take place to change automatically; avoided feedback regulation effect complicated in the complete synthesis approach; and matrix is formed simple, helps the product separation purifying.But glutamyltranspeptidase content and vigor in microbial cells is all lower, and the separation and purification difficulty, has restricted the development and the application of enzyme transforming process.People such as nineteen forty-two Bovarnick find that first bacillus can accumulate γ-PGA in substratum, have opened the research history of Production by Microorganism Fermentation γ-PGA thus.Compare with chemical synthesis method and enzyme transforming process, Production by Microorganism Fermentation γ-PGA has that production process is controlled easily, fermentation yield is stable, extraction yield is high, target product output is higher and molecular weight of product is suitable, advantages of environment protection, has become research and has produced main method and the approach of γ-PGA.In decades, the research that utilizes Production by Microorganism Fermentation γ-PGA is the focus that people pay close attention to always, correlative study is very active, and content relates to bacterial screening, fermention medium optimization, fermentation condition control, separation purification, γ-PGA structure and the research of character of γ-PGA, the many aspects such as synthesis mechanism of γ-PGA.At present, Japan has realized that γ-PGA produces in batches.Domesticly carry out the research of γ-PGA than later, in recent years, some R﹠D institutions of universities and colleges begin screening, selection by mutation, Optimizing Conditions of Fermentation, biosynthetic pathway and mechanism of the generation bacterium of γ-PGA and batch fermentation production etc. are studied, and have obtained some interim achievements in research.
Since the reserves of petroleum resources are limited and human survival to the requirement of environmental protection, sustainable economic development, bioabsorbable polymer material more and more is subjected to people's attention.Though, report related microorganism fermentation method carries out the many of γ-PGA research in the domestic literature at present, but research contents repeats, obtained result of study still has suitable distance from industrial applications, and existence lacks good γ-PGA production bacterial strain, high efficiency separation is extracted thorny problems such as supporting research of highly purified γ-PGA and method deficiency from fermented liquid.
Summary of the invention
The present invention will deal with problems and provide a plant height and produce gamma-polyglutamic acid-(the bacillus amyloliquefaciens bacterial strain of γ-PGA).
The genus bacillus of high yield gamma-polyglutamic acid-of the present invention, it is characterized in that: this bacterial strain called after bacillus amyloliquefaciens (Bacillus amyloliquefaciens) SFPG-123, described bacterial strain on November 16th, 2009 be deposited in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", preserving number are CGMCC N0.3447; Wherein the high molecular polymer that is produced is accredited as gamma-polyglutamic acid-by analysis.
The mycology feature of above-mentioned bacillus amyloliquefaciens (Bacillus amyloliquefaciens) SFPG-123 CGMCC NO.3447 is:
Bacterial strain CGMCC N0.3447 cultivates the 24h observation for dull and stereotyped last 37 ℃ in solid culture (isolation medium), and bacterium colony is rounded, projection, and neat in edge, flash of light, colourless or slightly yellow, translucent to transparent, diameter 3mm～7mm, smooth surface, thickness can wire drawings.
Bacterial strain CGMCC N0.3447 slant culture 12h observes, and thalline is rod-short, big or small 1.5 μ m * 1.0 μ m, and gramstaining is positive, and is movable, and the electron microscopic observation thalline is formed with tangible tunicle (see figure 1) outward.
Bacterial strain CGMCC N0.3447 physiological and biochemical property is: bacterial strain SFPG-123 optimum growth temperature is 32～37 ℃, can grow for 50 ℃; Have salt tolerance preferably, can in the substratum that contains 11%NaCl concentration, grow well-grown in the substratum that contains 7%NaCl concentration; This bacterium can well utilize starch and casein, can produce very big transparent circle on flat board; Can utilize Citrate trianion, liquefy gelatin does not utilize propionic salt, and V.P. tests positive, and M.R. tests negative.
The Physiology and biochemistry experimental result sees table 1 for details.
The part physiological and biochemical property of table 1 bacterial strain CGMCC N0.3447
Annotate: "+" well-grown or be positive; "-" do not grow or is negative.
The above-mentioned solid medium that is used for the colonial morphology observation is formed (g/L) and is: Trisodium Citrate 10, Sodium Glutamate 20, yeast extract paste 5, K
2HPO
40.5, MgSO
47H
2O 0.5, agar 18, pH value 7.0.
The above-mentioned slant medium that is used for the thalli morphology observation is formed (g/L) and is: peptone 10, yeast extract paste 5, sodium-chlor 10, agar 20, pH7.0-7.2, distilled water preparation.
" the common bacteria system identification handbook " that the experimental technique of above-mentioned observation of morphological is write with reference to the elegant pearl in east, Cai Miaoying etc., Science Press, 2001, first version, p353-363.
" the common bacteria system identification handbook " that above-mentioned physiological and biochemical test substratum and experimental technique are write with reference to the elegant pearl in east, Cai Miaoying etc., Science Press, 2001, first version, p364-398.
Above-mentioned bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CGMCC NO.3447 was from obtaining with the ordinary method separation screening the broad bean paste of traditional method brew, and described bacterial strain has been deposited in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center " on November 16th, 2009.
The application of the genus bacillus of product gamma-polyglutamic acid-of the present invention in the preparation gamma-polyglutamic acid-.
Wherein, the described method for preparing gamma-polyglutamic acid-is:
Shake flask fermentation: get the 35-40 ℃ of fresh bacterial classification CGMCC NO.3447 inclined-plane inoculation liquid seed culture medium of cultivating 1-2 days, cultivated 10-16 hour for 37 ℃, inoculation shake flask fermentation substratum, 35-40 ℃ shake flask fermentation 48-72 hour; Or, 37 ℃ of fresh bacterial classification CGMCC NO.3447 inclined-planes inoculation liquid seed culture mediums of cultivating 1-2 days were cultivated 10-18 hour for 35-40 ℃, be linked in the fermentor tank 35～40 ℃ of stir culture 48～60 hours of ventilating by the inoculum size of 1-5%; Get fermented liquid 1L then, 2～3 times of thin ups, the centrifugal 20～25min of 8000r/min remove thalline and other graininess impurity; Get supernatant liquor, the volume ratio that adds its 2～4 times of volumes is 95% ethanol 3～8 ℃ of precipitations 10-20 hour, the more centrifugal 10～15min collecting precipitation of 8000r/min thing; Be 95% washing with alcohol throw out 2-3 time with volume ratio again, add deionized water then throw out is dissolved,, obtain CGMCC NO.3447 bacterial strain and produce the gamma-polyglutamic acid-crude product through conventional lyophilize;
The culture temperature of above-mentioned gamma-polyglutamic acid-fermentation is preferably 37 ℃.
Above-mentioned on liquid seed culture medium incubation time be preferably 12-14 hour.
The aforesaid liquid seed culture medium consists of:
Trisodium Citrate 10, Sodium Glutamate 20, yeast extract paste 5, K
2HPO
40.5, MgSO
47H
2O 0.5, MnSO
4H
2O 0.1, pH value 7.0;
Above-mentioned fermention medium consists of:
Trisodium Citrate 15, Sodium Glutamate 20, ammonium chloride 7, bean cake powder 10, K
2HPO
40.5, MgSO
47H
2O 0.5, MnSO
4H
2O 0.1, CaCl
22H
2O 0.15, FeCl
36H
2O 0.04, pH value 7.0.
The gamma-polyglutamic acid-crude product is analyzed
Take by weighing the gamma-polyglutamic acid-crude product 2g that produces as stated above, with an amount of water dissolution, draw 10mL in little triangular flask, add the hydrochloric acid of equal-volume 11.8mol/L, 121 ℃, high temperature and high pressure hydrolysis 3h obtains hydrolyzed solution.Adopt the 853HITACHI automatic analyzer for amino acids to measure the amino acid kind and the content of hydrolyzed solution.
Analytical results shows: hydrolyzation sample two-story valley histidine content accounts for 90% (the amino acid analysis spectrogram is seen Fig. 2) of total amino acid content, illustrates that CGMCC NO.3447 high molecular polymer that bacterial strain produces of the present invention is a polyglutamic acid.
Screening and separating is to the bacterial strain SFPG-123 of strain product gamma-polyglutamic acid-from Chinese traditional fermented food broad bean paste in the present invention, and this bacterial strain is accredited as bacillus amyloliquefaciens (Bacillusamyloliquefaciens) through Physiology and biochemistry experiment and 16sRNA gene sequencing.Experiment test shows: bacillus amyloliquefaciens provided by the invention (Bacillus amyloliquefaciens) SFPG-123 CGMCC NO.3447 can transform L-glutamic acid, citric acid etc. and generate gamma-polyglutamic acid-; The gamma-polyglutamic acid-productive rate can reach 15-20g/L in the unit volume fermented liquid, is that a strain has the bacterial strain that research and development are worth.
Description of drawings
Bacillus amyloliquefaciens provided by the invention (Bacillus amyloliquefaciens) bacterial strain SFPG-123, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on November 16th, 2009, preservation address: No. 3 postcodes in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City: 100101, its deposit number is CGMCC NO.3447.
Fig. 1 shows bacillus amyloliquefaciens CGMCC NO.3447 cellular form of the present invention (* 29000).
Fig. 2 shows that bacillus amyloliquefaciens CGMCC NO.3447 of the present invention produces gamma-polyglutamic acid-hydrolysate amino acid analysis collection of illustrative plates.
Embodiment
Embodiment 1 produces the gamma-polyglutamic acid-bacterial strain screening
Get commercially available according to a conventional method or sample such as production scene broad bean paste, fermented soya bean, sauce beans, fermented bean curd, natto, get the 2g sample under the condition of aseptic technique, put into the 250mL triangular flask that the 30mL sterilized water is housed, leave standstill 30min after the concussion, boiling water bath boils 5min.Getting above-mentioned sample 1mL is inoculated in the 250mL triangular flask that the 30mL enrichment medium is housed in 37 ℃, 180r/min shaking table cultivation 24h, again the pregnant solution dilution is applied to plate culture medium, 37 ℃ of constant temperature culture are waited to grow behind single bacterium colony the picking thickness and have obvious stringy bacterium colony to move connecing the 37 ℃ of constant temperature culture in inclined-plane.Then these slant strains are carried out again the shake flask fermentation screening, 30mL shake flask fermentation substratum is loaded in the 250mL triangular flask, 1 connects 1 bottle graft and goes into 1～2 ring slant strains, 37 ℃, 180r/min shaking culture are about 48 hours, survey the viscosity of fermented liquid then, select the high bacterial strain of viscosity, final screening obtains a plant height and produces gamma-polyglutamic acid-(bacterial strain of γ-PGA), called after bacillus amyloliquefaciens (Bacillus amyloliquefaciens) SFPG-123.This bacterial strain has been deposited on November 16th, 2009 that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", preserving number are CGMCC N0.3447.
Embodiment 2 utilizes CGMCC NO.3447 strain fermentation to prepare gamma-polyglutamic acid-
Shake flask fermentation: get the 35-40 ℃ of fresh bacterial classification CGMCC NO.3447 inclined-plane inoculation liquid seed culture medium of cultivating 1-2 days, cultivated 10-16 hour for 37 ℃, inoculation shake flask fermentation substratum, 35-40 ℃ shake flask fermentation 48-72 hour.
Or liquid seed culture mediums are inoculated on 37 ℃ of fresh bacterial classification CGMCC NO.3447 inclined-planes of cultivating 1-2 days cultivated 10-18 hour for 35-40 ℃, the inoculum size of 1-5% is linked in the fermentor tank by volume, 35～40 ℃ of stir culture 48～60 hours of ventilating finish fermentation.Get the 1L fermented liquid then, 2～3 times of thin ups, the centrifugal 20min of 8000r/min removes thalline and other graininess impurity, gets supernatant liquor, adds 95% ethanol sedimentation of 2～4 times of volumes, low temperature spends the night, the centrifugal 10min collecting precipitation of 8000r/min thing is used an amount of washing with alcohol throw out 2-3 time more again, adds appropriate amount of deionized water with resolution of precipitate, through lyophilize, obtain bacterial strain CGMCC NO.3447 and produce the gamma-polyglutamic acid-crude product.
Embodiment 3 utilizes CGMCC NO.3447 strain fermentation to prepare gamma-polyglutamic acid-
Shake flask fermentation: get 37 ℃ of fresh bacterial classification CGMCC NO.3447 inclined-planes inoculation liquid seed culture mediums of cultivating 2 days, cultivated 15-16 hour for 37 ℃, inoculation shake flask fermentation substratum, 37 ℃ of shake flask fermentations 56 hours.
Or 37 ℃ of fresh bacterial classification CGMCC NO.3447 inclined-planes inoculation liquid seed culture mediums of cultivating 2 days were cultivated 14 hours for 37 ℃, 5% inoculum size is linked in the fermentor tank by volume, and 37 ℃ of stir culture 50 hours of ventilating finish fermentation.Get the 1L fermented liquid then, 2 times of thin ups, the centrifugal 20min of 8000r/min removes thalline and other graininess impurity, get supernatant liquor, 95% the ethanol that adds 3 times of volumes is at 5 ℃ of precipitations 15 hours, the centrifugal 10min collecting precipitation of 8000r/min thing again, use an amount of washing with alcohol throw out 3 times again, add appropriate amount of deionized water with resolution of precipitate,, obtain bacterial strain CGMCCNO.3447 and produce the gamma-polyglutamic acid-crude product through lyophilize.
The gamma-polyglutamic acid-crude product is analyzed
Take by weighing the gamma-polyglutamic acid-crude product 2g that produces as stated above, with an amount of water dissolution, draw 10mL in little triangular flask, add the hydrochloric acid of equal-volume 11.8mol/L, 121 ℃, high temperature and high pressure hydrolysis 3h obtains hydrolyzed solution.Adopt the 853HITACHI automatic analyzer for amino acids to measure the amino acid kind and the content of hydrolyzed solution.
Analytical results shows: hydrolyzation sample two-story valley histidine content accounts for 90% (the amino acid analysis spectrogram is seen Fig. 2) of total amino acid content, illustrates that CGMCC NO.3447 high molecular polymer that bacterial strain produces provided by the invention is a polyglutamic acid.
Above-mentioned product gamma-polyglutamic acid-bacterial strain enrichment medium consists of (g/L): Trisodium Citrate 16, Sodium Glutamate 20, ammonium chloride 7, yeast extract paste 5, K
2HPO
40.5, MgSO
47H
2O 0.5, MnSO
4H
2O 0.1, CaCl
22H
2O 0.15, FeCl
36H
2O0.04, pH value 7.0.
Above-mentioned product gamma-polyglutamic acid-bacterial strain plate isolation substratum consists of (g/L): Trisodium Citrate 10, Sodium Glutamate 20, ammonium chloride 7, yeast extract paste 5, K
2HPO
40.5, MgSO
47H
2O 0.5, MnSO
4H
2O0.1, agar 18, pH value 7.0.
Above-mentioned product gamma-polyglutamic acid-bacterial strain shake flask fermentation substratum consists of (g/L):
Trisodium Citrate 15, Sodium Glutamate 20, ammonium chloride 7, bean cake powder 10, K
2HPO
40.5, MgSO
47H
2O 0.5, MnSO
4H
2O 0.1, CaCl
22H
2O 0.15, FeCl
36H
2O 0.04, pH value 7.0.
Claims (5)
1. the genus bacillus of gamma-polyglutamic acid-is produced in a strain, it is characterized in that: this bacterial strain called after bacillus amyloliquefaciens (Bacillusamyloliquefaciens) SFPG-123, described bacterial strain has been deposited on November 16th, 2009 that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", preserving number are CGMCC NO.3447.
2. the application of the genus bacillus of the described product gamma-polyglutamic acid-of claim 1 in the preparation gamma-polyglutamic acid-.
3. application as claimed in claim 2, its method for preparing gamma-polyglutamic acid-is:
Shake flask fermentation: get the 35-40 ℃ of fresh bacterial classification CGMCC NO.3447 inclined-plane inoculation liquid seed culture medium of cultivating 1-2 days, cultivated 10-16 hour for 37 ℃, inoculation shake flask fermentation substratum, 35-40 ℃ shake flask fermentation 48-72 hour; Or, 37 ℃ of fresh bacterial classification CGMCC NO.3447 inclined-planes inoculation liquid seed culture mediums of cultivating 1-2 days were cultivated 10-18 hour for 35-40 ℃, be linked in the fermentor tank 35～40 ℃ of stir culture 48～60 hours of ventilating by the inoculum size of 1-5%; Get fermented liquid 1L, 2～3 times of thin ups, centrifugal 20～the 25min of 8000r/min removes thalline and other graininess impurity, get supernatant liquor, 95% the ethanol that adds its 2～4 times of volumes is at 3～8 ℃ of precipitations 10-20 hour, the centrifugal 10～15min collecting precipitation of 8000r/min thing again, be 95% washing with alcohol throw out 2-3 time with volume ratio again, add deionized water then throw out is dissolved,, obtain CGMCC NO.3447 bacterial strain and produce the gamma-polyglutamic acid-crude product through conventional lyophilize;
The aforesaid liquid seed culture medium consists of:
Trisodium Citrate 10g/L, Sodium Glutamate 20g/L, yeast extract paste 5g/L, K
2HPO
40.5g/L, MgSO
47H
2O0.5g/L, MnSO
4H
2O 0.1g/L, pH value 7.0;
Above-mentioned fermention medium consists of:
Trisodium Citrate 15g/L, Sodium Glutamate 20g/L, ammonium chloride 7g/L, bean cake powder 10g/L, K
2HPO
40.5g/L, MgSO
47H
2O0.5g/L, MnSO
4H
2O0.1g/L, CaCl
22H
2O 0.15g/L, FeCl
36H
2O 0.04g/L, pH value 7.0.
4. application as claimed in claim 3 is characterized in that: the culture temperature of described gamma-polyglutamic acid-fermentation is 37 ℃.
5. application as claimed in claim 3 is characterized in that: described on liquid seed culture medium incubation time be 12-14 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010150439 CN101875910A (en) | 2010-04-20 | 2010-04-20 | Bacillus amyloliquefaciens for producing gamma-polyglutamic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010150439 CN101875910A (en) | 2010-04-20 | 2010-04-20 | Bacillus amyloliquefaciens for producing gamma-polyglutamic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101875910A true CN101875910A (en) | 2010-11-03 |
Family
ID=43018595
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010150439 Pending CN101875910A (en) | 2010-04-20 | 2010-04-20 | Bacillus amyloliquefaciens for producing gamma-polyglutamic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101875910A (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103804067A (en) * | 2013-07-22 | 2014-05-21 | 河海大学 | Biological agent for supporting calcium to facility plantation crops as well as preparation method and application of biological agent |
| CN106947724A (en) * | 2017-05-22 | 2017-07-14 | 中国科学院成都生物研究所 | A kind of method of increase γ polyglutamic acid zymotic fluid dissolved oxygens |
| CN107058151A (en) * | 2016-11-29 | 2017-08-18 | 广东森度生态农业科技有限公司 | A kind of microbial bacteria with fermentation water retaining function and its application |
| CN107118990A (en) * | 2017-05-22 | 2017-09-01 | 中国科学院成都生物研究所 | Its production γ PGA of high yield γ PGA bacterial strains and application method |
| CN108048499A (en) * | 2018-02-09 | 2018-05-18 | 烟台市佳益有机肥料有限公司 | A kind of method of solid fermentation production gamma-polyglutamic acid |
| CN109477123A (en) * | 2016-08-25 | 2019-03-15 | 花王株式会社 | The production method of poly-gamma-glutamic acid |
| CN110637678A (en) * | 2019-09-30 | 2020-01-03 | 常熟理工学院 | Improvement method for covering soil of cultivated agaricus bisporus |
| WO2020036400A1 (en) * | 2018-08-13 | 2020-02-20 | 씨제이제일제당(주) | BACILLUS AMYLOLIQUEFACIENS CJBA1 AND METHOD FOR PRODUCING γ-POLYGLUTAMIC ACID USING SAME |
| KR20200019099A (en) * | 2018-08-13 | 2020-02-21 | 씨제이제일제당 (주) | Bacillus amyloliquefaciens CJBA1 and method of producing gamma-polyglutamic acid using the same |
| CN111500653A (en) * | 2020-05-26 | 2020-08-07 | 樟树市狮王生物科技有限公司 | Production process of polyglutamic acid |
| CN114958646A (en) * | 2022-04-02 | 2022-08-30 | 安徽粤智徽源生物科技有限公司 | Blue bacillus amyloliquefaciens for producing polyglutamic acid |
| CN115786177A (en) * | 2021-11-11 | 2023-03-14 | 华中农业大学 | Bacillus amyloliquefaciens DT5 and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101109010A (en) * | 2007-07-17 | 2008-01-23 | 秦皇岛领先科技发展有限公司 | Mycopremna generating gamma- polyglutamic acid and culturing method thereof |
| CN101508962A (en) * | 2008-07-21 | 2009-08-19 | 南开大学 | Non-glutamic acid dependent form gamma-polyglutamic acid synthesis bacterium and fermentation method |
-
2010
- 2010-04-20 CN CN 201010150439 patent/CN101875910A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101109010A (en) * | 2007-07-17 | 2008-01-23 | 秦皇岛领先科技发展有限公司 | Mycopremna generating gamma- polyglutamic acid and culturing method thereof |
| CN101508962A (en) * | 2008-07-21 | 2009-08-19 | 南开大学 | Non-glutamic acid dependent form gamma-polyglutamic acid synthesis bacterium and fermentation method |
Non-Patent Citations (1)
| Title |
|---|
| 《食品与发酵工业》 20081231 邵丽等 产聚丫一谷氨酸菌株的筛选及鉴定 81-84 1-5 , 第6期 2 * |
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103804067A (en) * | 2013-07-22 | 2014-05-21 | 河海大学 | Biological agent for supporting calcium to facility plantation crops as well as preparation method and application of biological agent |
| CN109477123A (en) * | 2016-08-25 | 2019-03-15 | 花王株式会社 | The production method of poly-gamma-glutamic acid |
| CN109477123B (en) * | 2016-08-25 | 2020-07-10 | 花王株式会社 | Method for producing poly-gamma-glutamic acid |
| CN107058151A (en) * | 2016-11-29 | 2017-08-18 | 广东森度生态农业科技有限公司 | A kind of microbial bacteria with fermentation water retaining function and its application |
| CN107058151B (en) * | 2016-11-29 | 2021-08-20 | 广东森度生态农业科技有限公司 | Microbial bacterium with fermentation water retention function and application thereof |
| CN106947724B (en) * | 2017-05-22 | 2020-05-19 | 中国科学院成都生物研究所 | Method for increasing dissolved oxygen of gamma-polyglutamic acid fermentation liquor |
| CN106947724A (en) * | 2017-05-22 | 2017-07-14 | 中国科学院成都生物研究所 | A kind of method of increase γ polyglutamic acid zymotic fluid dissolved oxygens |
| CN107118990A (en) * | 2017-05-22 | 2017-09-01 | 中国科学院成都生物研究所 | Its production γ PGA of high yield γ PGA bacterial strains and application method |
| CN107118990B (en) * | 2017-05-22 | 2020-03-27 | 中国科学院成都生物研究所 | High-yield gamma-PGA strain and method for producing gamma-PGA by using same |
| CN108048499A (en) * | 2018-02-09 | 2018-05-18 | 烟台市佳益有机肥料有限公司 | A kind of method of solid fermentation production gamma-polyglutamic acid |
| KR20200019099A (en) * | 2018-08-13 | 2020-02-21 | 씨제이제일제당 (주) | Bacillus amyloliquefaciens CJBA1 and method of producing gamma-polyglutamic acid using the same |
| WO2020036400A1 (en) * | 2018-08-13 | 2020-02-20 | 씨제이제일제당(주) | BACILLUS AMYLOLIQUEFACIENS CJBA1 AND METHOD FOR PRODUCING γ-POLYGLUTAMIC ACID USING SAME |
| KR102155158B1 (en) | 2018-08-13 | 2020-09-11 | 씨제이제일제당 주식회사 | Bacillus amyloliquefaciens CJBA1 and method of producing gamma-polyglutamic acid using the same |
| CN110637678A (en) * | 2019-09-30 | 2020-01-03 | 常熟理工学院 | Improvement method for covering soil of cultivated agaricus bisporus |
| CN111500653A (en) * | 2020-05-26 | 2020-08-07 | 樟树市狮王生物科技有限公司 | Production process of polyglutamic acid |
| CN115786177A (en) * | 2021-11-11 | 2023-03-14 | 华中农业大学 | Bacillus amyloliquefaciens DT5 and application thereof |
| CN115786177B (en) * | 2021-11-11 | 2024-08-09 | 华中农业大学 | Bacillus amyloliquefaciens DT5 and application thereof |
| CN114958646A (en) * | 2022-04-02 | 2022-08-30 | 安徽粤智徽源生物科技有限公司 | Blue bacillus amyloliquefaciens for producing polyglutamic acid |
| CN114958646B (en) * | 2022-04-02 | 2023-12-05 | 安徽粤智徽源生物科技有限公司 | Bacillus amyloliquefaciens blue for producing polyglutamic acid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101875910A (en) | Bacillus amyloliquefaciens for producing gamma-polyglutamic acid | |
| CN101948785B (en) | Gamma-polyglutamic acid producing bacterium and method for preparing gamma-polyglutamic acid and salts thereof by using gamma-polyglutamic acid producing bacterium | |
| CN108865951B (en) | A kind of general bacterium of camel thorn and its microbial inoculum and application | |
| CN109161492A (en) | A kind of gamma-polyglutamic acid generating bacterium and the method for efficiently synthesizing gamma-polyglutamic acid | |
| CN101109010B (en) | Mycopremna generating gamma- polyglutamic acid and culturing method thereof | |
| CN106635869B (en) | A method of producing surfactin using bacillus amyloliquefaciens | |
| CN102911974B (en) | Method for producing gamma-polyglutamic acid though hot fermentation of bacillus subtilis | |
| CN101948769B (en) | Bacteria YXY-C1 for preparing gamma-polyglutamic acid by solid fermentation and products thereof | |
| CN101486977B (en) | Bacillus subtilis and method for preparing gamma-polyglutamic acid by using the same | |
| CN101348803B (en) | Artificial nutrient medium for Phellinus linteus fluid culture and method for fermentation of Phellinus linteus polysaccharide | |
| CN102220248B (en) | Bacterial strain for producing PMLA [Poly (Beta-L-malic acid)] and method for producing PMLA by fermentation of bacterial strain | |
| CN104232504A (en) | Strain for synthesizing gamma-polyglutamic acid and method for efficiently preparing gamma-polyglutamic acid by using strain | |
| CN102597251A (en) | A process for integrated production of ethanol and seaweed sap from kappaphycus alvarezii | |
| CN101838619A (en) | Mutagenized strain Bacillus licheniformis TKPG091 for generating great amount of gamma-poly glutamic acid | |
| CN107699594A (en) | A kind of method that γ polyglutamic acids are produced using bacillus licheniformis | |
| CN109609408B (en) | Gamma-polyglutamic acid high-yield strain and method for preparing gamma-polyglutamic acid by using strain for liquid fermentation | |
| CN102586151B (en) | High-yield polysaccharide strain and method for producing polysaccharide by utilizing strain through fermentation | |
| CN105017086A (en) | Separation and purification method for L-citrulline | |
| CN106047713B (en) | The basket bacteria strain Li-93 of one plant of thermophilic pine and its application | |
| CN102533885A (en) | Method for producing gamma-polyglutamic acid through adding sodium chloride (NaCl) in fermentation process | |
| CN102174448B (en) | Streptomyces albidoflavus and application thereof in preparation of polylysine and polydiaminobutyric acid | |
| CN109182404A (en) | A method of utilizing stream plus sugar industry gamma-polyglutamic acid when the lichen bacillus ferments | |
| CN102994430A (en) | Bacterial cellulose production strain and application thereof | |
| CN110129225A (en) | γ~polyglutamic acid producing strains and breeding prepare γ~polyglutamic acid method | |
| CN108220352A (en) | A kind of method of raw material fermentation production gamma-polyglutamic acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20101103 |