CN101824384A - Method for cultivating chrysophyceae by utilizing cynoglossus semilaevis cultivation waste water - Google Patents
Method for cultivating chrysophyceae by utilizing cynoglossus semilaevis cultivation waste water Download PDFInfo
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- CN101824384A CN101824384A CN 201010146768 CN201010146768A CN101824384A CN 101824384 A CN101824384 A CN 101824384A CN 201010146768 CN201010146768 CN 201010146768 CN 201010146768 A CN201010146768 A CN 201010146768A CN 101824384 A CN101824384 A CN 101824384A
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Abstract
The invention discloses a method for cultivating chrysophyceae by utilizing cynoglossus semilaevis cultivation waste water, including the steps of cynoglossus semilaevis cultivation waste water precipitation or filtration, mixed culture solution preparation and isochrysis zhanjiangensis cultivation. The invention adopts culture solution taking cynoglossus semilaevis cultivation waste water as main body, thus greatly reducing cultivation cost, and cultivation waste water purification is realized and carbon emission is reduced while the microalgae is obtained. Besides, cultivation water quality is improved while isochrysis zhanjiangensis with higher economic value is obtained, and the invention has better economic and social benefits.
Description
Technical field
The present invention relates to utilize a kind of aquiculture waste water to cultivate the technology of chrysophyceae, relate in particular to a kind of method of utilizing cynoglossus semilaevis cultivation waste water to cultivate chrysophyceae.
Background technology
Aquiculture waste water, be rich in nutritive element and organic matters such as nitrogen, phosphorus, exceeding standard and excessively discharging of it is an important factor that causes body eutrophication and peripheral water environment to pollute, although developed many treatment technology and equipment at present at breeding wastewater, but because correlation technique is improved and ripe and higher running cost and energy consumption not enough, practical application effect is unsatisfactory.
Zhanjiang Isochrysis galbana (Isochrysis zhan jiangensis) is a kind of important ocean unit cell algae that is widely used, and is the high-quality bait of bivalve and sea cucumbers larva.Therefore, be one of important step of aquatic animal seedling system work to the cultivation of Zhanjiang Isochrysis galbana bait, laboratory and producing is gone up the abundant f/2 nutrient solution of element such as main nitrogenous, the phosphorus that adopts artificial preparation and is cultivated at present.Because nutritive element and trace element are numerous in the nutrient solution, not only preparation trouble has also increased the cultivation cost.
Discover that little algae such as chrysophyceae can absorb, consume nutritive element and organic matters such as nitrogen in the water body, phosphorus, and can absorb water body and carbon dioxide in air is carried out photosynthesis.Therefore, utilize breeding wastewater to cultivate economic little algaes such as chrysophyceae, can not only purifying aquaculture waste water, reduce carbon emission, and little algae of results also can be used for the exploitation of bait or the feed of aquatic animal, this had both reduced the processing costs of breeding wastewater, and also the production for economic little algaes such as chrysophyceae provides a development approach cheaply, can play the effect that kills two birds with one stone.
Summary of the invention
The object of the present invention is to provide the lower cynoglossus semilaevis cultivation waste water that utilizes of a kind of cost of development to cultivate the method for chrysophyceae.
For achieving the above object, technical scheme of the present invention is as follows:
The present invention is a kind of method of utilizing cynoglossus semilaevis cultivation waste water to cultivate chrysophyceae, and it may further comprise the steps:
(1) will cynoglossus semilaevis cultivation waste water precipitation or filter after be made into mixed-culture medium: add the material of following content at cynoglossus semilaevis cultivation waste water,
SODIUMNITRATE content is 0-15mg/L;
The biphosphate sodium content is 0-1mg/L;
Ironic citrate content is 0-1mg/L;
Sodium bicarbonate content is 0-0.5g/L;
Vitamins B
1Content is 0-100ug/L;
Vitamins B
12Content is 0-100ng/L;
(2) cultivate Zhanjiang Isochrysis galbana: with the mixed-culture medium that is made into, under 0-30 ℃ of temperature, pH4-8, more than the illumination 500LX-10000LX, the initial inoculation density of Zhanjiang Isochrysis galbana is to cultivate more than the 0.002-2mg/mL to obtain a large amount of Zhanjiang Isochrysis galbana in 5-30 days.
Compare with existing culture technique, outstanding advantage of the present invention is to have adopted the nutrient solution based on cynoglossus semilaevis cultivation waste water, thereby has reduced the cultivation cost significantly, and when obtaining this little algae, realizes the purification to breeding wastewater, has reduced carbon emission.
Method in the chief editor who spreads out according to thunder " cultivation water environment chemical experiment ", to the nitrogen in the breeding wastewater, indexs such as phosphorus are measured (nitric nitrogen employing zinc-cadmium reduction method, ammonia-state nitrogen adopts the indophenols blue laws, nitrite nitrogen adopts diazonium-azo light-intensity method, active phosphorus adopts molybdenum-antimony-anti-method), the result shows, growth (as shown in Figure 1) along with Zhanjiang Isochrysis galbana, nitric nitrogen in the water body, nitrite nitrogen, the content of nutritive elements such as active phosphorus declines to a great extent (as shown in Figure 2), cultivation through 11 days, chrysophyceae reaches 78.0% to the clearance rate of active phosphorus, clearance rate to nitric nitrogen is 84.7%, clearance rate to nitrite nitrogen is 62.3%, ammonia-state nitrogen was then just removed fully at the 2nd day, thereby reduced the eutrophication level of aquaculture water preferably.
The present invention utilizes cynoglossus semilaevis cultivation waste water to cultivate Zhanjiang Isochrysis galbana, when improving cultivation water, has also obtained to have Zhanjiang Isochrysis galbana of higher economic worth, has better economic and social benefit.
The present invention is further illustrated below in conjunction with the drawings and specific embodiments.
Description of drawings
Fig. 1 is the growth curve chart of Zhanjiang of the present invention Isochrysis galbana in cynoglossus semilaevis cultivation waste water;
Fig. 2 is the purification graphic representation of Zhanjiang of the present invention Isochrysis galbana to cynoglossus semilaevis cultivation waste water.
Embodiment
With cynoglossus semilaevis cultivation waste water 1L precipitation or after filtering, add SODIUMNITRATE 7.5mg, SODIUM PHOSPHATE, MONOBASIC 0.5mg, ironic citrate 0.5mg, sodium bicarbonate content are 0.25g, vitamins B
150ug, vitamins B
1250ng adds Zhanjiang Isochrysis galbana algae kind 10mg again, and under 20 ℃ of temperature, pH7, illumination 5000LX cultivate down can obtain a large amount of Zhanjiang Isochrysis galbana in 10 days.
With cynoglossus semilaevis cultivation waste water 800mL precipitation or after filtering, add SODIUMNITRATE 0mg, SODIUM PHOSPHATE, MONOBASIC 0mg, ironic citrate 0mg, sodium bicarbonate content are 0g, vitamins B
110ug, vitamins B
1210ng adds Zhanjiang Isochrysis galbana algae kind 1.6mg again, and under 0 ℃ of temperature, pH4, illumination 500LX cultivate down can obtain a large amount of Zhanjiang Isochrysis galbana in 5 days.
Embodiment 3
With cynoglossus semilaevis cultivation waste water 1L precipitation or after filtering, add SODIUMNITRATE 15mg, SODIUM PHOSPHATE, MONOBASIC 1mg, ironic citrate 1mg, sodium bicarbonate content are 0.5g, vitamins B
1100ug, vitamins B
12100ng adds Zhanjiang Isochrysis galbana algae kind 2g again, and under 30 ℃ of temperature, pH8, illumination 10000LX cultivate down and promptly obtained a large amount of Zhanjiang Isochrysis galbana in 30 days.
With cynoglossus semilaevis cultivation waste water 1L precipitation or after filtering, add SODIUMNITRATE 8mg, SODIUM PHOSPHATE, MONOBASIC 0.4mg, ironic citrate 0.6mg, sodium bicarbonate content are 0.3g, vitamins B
160ug, vitamins B
1260ng adds Zhanjiang Isochrysis galbana algae kind 20mg again, and under 25 ℃ of temperature, pH6, illumination 6000LX cultivate down and promptly obtained a large amount of Zhanjiang Isochrysis galbana in 15 days.
Claims (1)
1. method of utilizing cynoglossus semilaevis cultivation waste water to cultivate chrysophyceae, it is characterized in that: it may further comprise the steps:
(1) will cynoglossus semilaevis cultivation waste water precipitation or filter after be made into mixed-culture medium: add the material of following content at cynoglossus semilaevis cultivation waste water,
SODIUMNITRATE content is 0-15mg/L;
The biphosphate sodium content is 0-1mg/L;
Ironic citrate content is 0-1mg/L;
Sodium bicarbonate content is 0-0.5g/L;
Vitamins B
1Content is 0-100ug/L;
Vitamins B
12Content is 0-100ng/L;
(2) cultivate Zhanjiang Isochrysis galbana: with the mixed-culture medium that is made into, under 0-30 ℃ of temperature, pH4-8, illumination 500LX-10000LX, the initial inoculation density of Zhanjiang Isochrysis galbana is that 0.002-2mg/mL cultivates and can obtain a large amount of Zhanjiang Isochrysis galbana in 5-30 days.
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| CN 201010146768 CN101824384A (en) | 2010-04-08 | 2010-04-08 | Method for cultivating chrysophyceae by utilizing cynoglossus semilaevis cultivation waste water |
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| CN 201010146768 CN101824384A (en) | 2010-04-08 | 2010-04-08 | Method for cultivating chrysophyceae by utilizing cynoglossus semilaevis cultivation waste water |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102453684A (en) * | 2010-10-27 | 2012-05-16 | 中国科学院大连化学物理研究所 | Culture method for improving golden algae biomass and grease content by using nitrogen source |
| CN106244489A (en) * | 2016-08-29 | 2016-12-21 | 佛山市艳晖生物科技有限公司 | A kind of chrysophyceae and the method for photosynthetic bacteria mixed fermentation |
| CN112251359A (en) * | 2020-12-21 | 2021-01-22 | 中国科学院烟台海岸带研究所 | Isochrysis for seawater seedling or cultivation in summer and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101209880A (en) * | 2006-12-29 | 2008-07-02 | 中国科学院大连化学物理研究所 | Method for purifying cultivation sewage by 'marine sponge-microalgae' integration system |
| CN101280271A (en) * | 2008-05-27 | 2008-10-08 | 蔡志武 | Production unit for microalgae industrialization and method for producing microalgae |
-
2010
- 2010-04-08 CN CN 201010146768 patent/CN101824384A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101209880A (en) * | 2006-12-29 | 2008-07-02 | 中国科学院大连化学物理研究所 | Method for purifying cultivation sewage by 'marine sponge-microalgae' integration system |
| CN101280271A (en) * | 2008-05-27 | 2008-10-08 | 蔡志武 | Production unit for microalgae industrialization and method for producing microalgae |
Non-Patent Citations (2)
| Title |
|---|
| 《Aquat. Living Resour.》 19981231 Hussenot et al. Open-air treatment of wastewater from land-based marine fish farms in extensive and intensive systems:current technology and future perspectives. 第11卷, 第4期 2 * |
| 《中国优秀博硕士学位论文全文数据库(硕士)》 20060315 马冬冬 基于微藻的光-膜组合式生物反应器处理海水养殖业废水 , 第3期 2 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102453684A (en) * | 2010-10-27 | 2012-05-16 | 中国科学院大连化学物理研究所 | Culture method for improving golden algae biomass and grease content by using nitrogen source |
| CN102453684B (en) * | 2010-10-27 | 2014-06-18 | 中国科学院大连化学物理研究所 | Culture method for improving golden algae biomass and grease content by using nitrogen source |
| CN106244489A (en) * | 2016-08-29 | 2016-12-21 | 佛山市艳晖生物科技有限公司 | A kind of chrysophyceae and the method for photosynthetic bacteria mixed fermentation |
| CN106244489B (en) * | 2016-08-29 | 2020-02-04 | 佛山市艳晖生物科技有限公司 | Method for mixed fermentation of chrysophyceae and photosynthetic bacteria |
| CN112251359A (en) * | 2020-12-21 | 2021-01-22 | 中国科学院烟台海岸带研究所 | Isochrysis for seawater seedling or cultivation in summer and application thereof |
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Open date: 20100908 |