CN101780147A - Anti-inflammatory and hypoglycemic mulberry leave extract and preparation method of preparation thereof - Google Patents
Anti-inflammatory and hypoglycemic mulberry leave extract and preparation method of preparation thereof Download PDFInfo
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Abstract
The invention relates to mulberry leave extract and a preparation method of a preparation thereof. The extract contains high concentration of mulberry leave alkaloid, flavone and polysaccharide and has obvious anti-inflammatory function and the function of reducing abnormal hyperglycemia to laboratory animals. Therefore, the mulberry leave extract can be popularized and used as a drug or a health-related product.
Description
Technical field
The present invention relates to a kind of Folium Mori antiinflammatory, blood sugar lowering preparation method of extract and preparation thereof, this extract contains flavones in mulberry leaves, alkaloid and the polysaccharide component of higher concentration, tangible antiinflammatory of this extract and preparation thereof and blood sugar lowering effect.
Background technology
Folium Mori are the leaf of F.C.Ficus Callosa Mulberry (Morus alba L.), its medical value sees Shennong's Herbal the earliest, and Folium Mori are cold in nature, bitter in the mouth, return lung, Liver Channel, have effect " Bencao Jingshu " records such as expelling wind and clearing away heat, cooling blood for improving eyesight: Folium Mori nature and flavor hardship, sweet, cold, thus sweet beneficial blood, so cold removing heat from blood, sweet cold being harmonious, so the therapeutic method to keep the adverse QI flowing downwards and tonifying YIN can make eye bright and cough-relieving again has the merit of tonification.Modern chemistry studies show that Folium Mori contain the multiple chemical substances favourable to health such as flavone and flavonoid glycoside, alkaloid, polysaccharide, sterol, volatile oil, aminoacid, vitamin, trace element.Pharmacological research shows that Folium Mori reach human body and are safety, and have multiple treatment functions such as blood sugar lowering, blood fat reducing, atherosclerosis, antiinflammatory, defying age, antitumor, antiviral.
The research report and the patent application of Folium Mori effective component extracts are very many, patent of invention ' preparation of a kind of Folium Mori total alkali extractum and preparation method thereof the ' (publication number of declaring as Hong Chunli: CN1430964A), introduced a kind of extracting method for the treatment of diabetes total alkaloids of Folium Mori extractum; Li Shufen waits patent of invention ' method of continuously extracting alkaloids, flavone and polysaccharide active components from the Folium Mori ' (publication number of declaring: CN101209284), introduced the method for extracting purifying biological alkali, flavone, polysaccharide composition from Folium Mori continuously.But, do not find the record of declaring of research report that Folium Mori antiinflammatory, blood sugar lowering effective ingredient are extracted simultaneously and patent at present as yet by document inquiry.
The objective of the invention is to develop a kind of Folium Mori antiinflammatory, blood sugar lowering extract and preparation thereof, enlarge the range of application of Folium Mori.
Summary of the invention
The invention discloses a kind of Folium Mori antiinflammatory, blood sugar lowering preparation method of extract and preparation thereof.This preparation method simple and feasible, preparation stabilization safety is very beneficial for suitability for industrialized production, by the animal pharmacodynamic experiment, proves that the extract that this preparation method is produced has significant antiinflammatory and hypoglycemic activity.
The research that Folium Mori antiinflammatory, blood sugar lowering effective ingredient extract does not simultaneously appear in the newspapers, and the present invention has used some know-how to improve antiinflammatory, the hypoglycemic activity of Folium Mori extract on the basis of adopting traditional extraction separation method.Concrete steps are as follows: after the Folium Mori medical material is suitably pulverized, with 10% to 95% aqueous methanol or ethanol, static or down dynamic, room temperature or heating (comprising backflow, microwave etc.) are extracted, the extraction solvent load is 5～15 times of medical material, extraction time is 1～3 time, after extracting liquid filtering merging, decompression or air-distillation recovery alcoholic solvent are lower than 20% to collection liquid alcohol content, continue evaporation and concentration to not having alcohol flavor (1.05～1.25/80 ℃ of relative densities), adding 2～6 times of amounts of water, after adding acid for adjusting pH=3～2, put 24 to 72 hours, and filtered.It is standby to filter insoluble matter oven drying at low temperature, the pulverizing of collecting.After the first mistake of filtrate polyamide fully adsorbs, cross cation exchange resin and fully adsorb.The washing of the polyamide of absorption filtrate is clean, with 10% to 95% aqueous methanol or ethanol extraction, it is closely neutral that extracting solution is regulated pH with acetic acid, reclaims alcoholic solvent after 4 hours for the vexed system of ammonia, and concentrating under reduced pressure becomes the pulverizing of extractum after drying standby.The washing of the ion exchange resin of absorption filtrate is clean, with 10% to 95% aqueous methanol or ethanol extraction, extracting solution reclaims alcoholic solvent to the vexed system of ammonia after 4 hours, and concentrating under reduced pressure becomes the pulverizing of extractum after drying standby.
Folium Mori coarse powder after the alcohol extraction is removed remaining alcoholic solution, use water extraction, extracting liquid filtering merges, reduction vaporization is concentrated to relative density greater than 1.20/80 ℃, add and to contain the alcohol amount and contain the alcohol amount greater than 85% alcoholic solution regulator solution and stir 50%～70%, place 24～72 hours after-filtration collecting precipitation things, oven drying at low temperature is pulverized standby.
With the aforementioned abundant mix homogeneously of material powder that makes promptly.
The extract that the present invention makes can add the adjuvant that country and industry standard and rules allow adding when producing medicine, healthy Related product preparation, such as: starch, sucrose, dextrin, mannitol, glycerol, silicon derivative, magnesium stearate, Pulvis Talci etc.
The extract that the present invention makes can be made any dosage form of medicine, healthy Related product by proper method, and these dosage forms comprise: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, buccal tablet, effervescent tablet, hard capsule, soft capsule, pill, drop pill, granule, medicinal tea, powder, unguentum, sublimed preparation, suspensoid, solution, injection, suppository, plaster, ointment, cream, spray, drop, patch etc.The preferential dosage form of selecting of the present invention is an oral formulations, as tablet, capsule, granule, mixture etc.
The extract that the present invention makes proves its beneficial effect by following pharmacodynamic experiment:
One, antiinflammatory action
1. experiment material
1.1 medicine: Folium Mori extract, with the preparation of example 1 method, water is made into desired concn before the experiment; Positive control drug dexamethasone (Zhejiang Province XianJu Pharmacy stock Co., Ltd, lot number 071010).Carrageenin (Nat'l Pharmaceutical ﹠ Biological Products Control Institute provides).1.2 animal: KM mice, 18～22g, ♂; The SD rat, body weight 110～140g, ♂.
1.3 instrument and reagent: EL204 ten thousand/gram electronic balance (Mettler-Toledo Instrument (Shanghai) Co., Ltd.).Lambda35UV Perkin Elmer (U.S. Po Jinaiermo company), LG16-W type high speed centrifuge (Beijing Medical Centrifugal Machine Factory).PV-200 toes volumetric measurement instrument (Chengdu TME Technology Co., Ltd.).
2. statistical procedures applied statistics software SPSS11.0 carries out the t check to the gained experimental data.
3. experimental technique
3.1 xylol causes the influence of mice auricle swelling degree: get 75 of mices, be divided into 5 groups at random, be respectively blank group (normal saline), positive controls (dexamethasone, 6mg/kg), the Folium Mori extract height (10g crude drug/kg), in (5g crude drug/kg), low (three dosage groups of 2g crude drug/kg).Gastric infusion, 1 time/d, the administration capacity is 20mL/kg, continuously 7d.60min behind the last medicine, each Mus is dripped in auris dextra with 0.02ml dimethylbenzene and causes inflammation, puts to death behind the 15min, cuts two ears along the auricle baseline.Lay round auricle at the same position of left and right ear respectively with 6mm diameter card punch, weigh, with left and right sides ear weight difference as the swelling degree.
3.2 Dichlorodiphenyl Acetate causes the influence of mouse peritoneal capillary permeability: get 60 of mices, be divided into 5 groups at random, be respectively blank group (normal saline), positive controls (dexamethasone, 6mg/kg), the Folium Mori extract height (10g crude drug/kg), in (5g crude drug/kg), low (three dosage groups of 2g crude drug/kg).Gastric infusion, 1 time/d, the administration capacity is 20mL/kg, continuously 7d.60min behind the last medicine, each caudal vein inject 0.25% Yi Wensilan 0.1ml/10g, simultaneously lumbar injection 0.6% acetum 0.1ml/10g.Put to death behind the 15min, inject 6ml normal saline flushing abdominal cavity immediately, cut off the abdominal cavity, leach the abdominal cavity eluate, the centrifugal 10min of 2000rpm gets supernatant and measures the OD value in the 590nm place.
3.3 on Carrageenan causes the influence of rat toes swelling: get 60 of rats, be divided into blank group (normal saline) at random, positive controls (dexamethasone, 4.5mg/kg), Folium Mori extract high dose (9g crude drug/kg), middle dosage (4g crude drug/kg), low dosage (three dosage groups of 2g crude drug/kg).Gastric infusion, 1 time/d, the administration capacity is 20ml/kg, continuously 7d.Behind the last administration 1h, it is subcutaneous that the 0.08mL1% carrageenin is injected the right back toes of rat, before measuring injection respectively with toes volumetric measurement instrument, injection back 1,2,3,4,5h rat toes be to the ankle joint volume, and calculate its swelling degree (swelling degree E (%)=(Vt-Vn)/Vn * 100%, wherein Vn and Vt represent respectively with toes volumetric values before and after the proinflammatory agent) with formula.
4. experimental result and analysis
The antiinflammatory experimental result sees the following form 1 and table 2:
Table 1 Folium Mori ethanol extract to auricle swelling degree (
, n=15) and the influence of abdominal cavity capillary permeability (
, n=12)
*P＜0.05
**P＜0.01
The influence of rat toes swelling due to the table 2 Folium Mori extract on Carrageenan (
, n=12)
*P＜0.05
**P＜0.01
Interpretation:
1) Folium Mori extract height, middle dosage group make mice respectively the degree of auricle edema by 0.0070 ± 0.0037 of blank group, be reduced to 0.0041 ± 0.0029 and 0.0028 ± 0.0022, high dose group is almost near the level of positive reagent dexamethasone.The degree that all can significantly alleviate dimethylbenzene inducing mouse auricle edema can be to the oozing out of anti-inflammatory acute stage (P＜0.01) (table 1).Table 1 is the result also show, the high, medium and low dosage group of Folium Mori extract makes acetic acid cause effect that the abdominal cavity capillary permeability increases and is reduced to respectively by 0.2124 ± 0.05800. of blank group: 0.1429 ± 0.0205,0.1315 ± 0.0460 and 0.1650 ± 0.03380. all have obvious inhibition acetic acid to cause the effect (P＜0.05～0.01) that the abdominal cavity capillary permeability increases, prompting all can oozing out the anti-inflammatory acute stage.
2) the high, medium and low dosage group of Folium Mori extract 1h, 2h, 3h, 4,5h after the last administration all has the degree (P＜0.05～0.01) that obviously suppresses rat toes swelling due to the carrageenin to some extent, and prompting all can oozing out the anti-inflammatory acute stage.
Conclusion: above experimental result explanation, this Folium Mori extract has good inhibitory effect to the experimental animal models inflammation.
Two, reduce experimental blood sugar increasing effect
1. experiment material
2.1 medicine: Folium Mori extract, with the preparation of example 1 method, water is made into desired concn before the experiment.Positive control drug Dimethyldiguanide hydrochloride enteric solubility tablet (Guizhou Shengjitang Pharmaceutical Co., Ltd., lot number: 20070501).
1.2 animal: KM mice, 18～22g, ♂.
1.3 instrument and reagent: EL204 ten thousand/gram electronic balance (Mettler-Toledo Instrument (Shanghai) Co., Ltd.), Lambda35UV Perkin Elmer (U.S. Po Jinaiermo company), LG16-W type high speed centrifuge (Beijing Medical Centrifugal Machine Factory); Blood glucose GOD-PAP method detection kit (Maike Tech Co., Ltd., Sichuan Prov., lot number: 0308091).
2. statistical procedures applied statistics software SPSS11.0 carries out the t check to the gained experimental data.
3. experimental technique and interpretation
Get the mice after water 24h is can't help in fasting, be divided into normal control group (blank group) and modeling matched group at random.Normal control group intraperitoneal injection of saline, the modeling matched group is pressed 65mg/kg body weight lumbar injection alloxan, (water 12h is can't help in fasting) each rathole socket of the eye venous plexus is got blood and is surveyed blood glucose value behind the 72h, selecting blood glucose value is modeling success mice (being called model control group) the mice of 10～33mmol/L, be divided into the alloxan model control group at random by blood sugar level, metformin positive controls (0.75g/kg), Folium Mori extract height (7g crude drug/kg), in (4g crude drug/kg), low (three dosage groups of 2g crude drug/kg).Gastric infusion, 1 time/d, the administration capacity is 20ml/kg, continuous 7d, normal control group and model control group are given with the capacity normal saline.(water 12h is can't help in fasting) 60min gets blood in the eye socket venous plexus after the last administration, presses blood sugar detection test kit time-and-motion study blood glucose value, the results are shown in following table 3:
Table 3 Folium Mori extract to the influence of alloxan hyperglycemia mouse blood sugar (
, n=12)
Experiment conclusion: this Folium Mori extract has obvious reduction effect to the blood glucose of alloxan hyperglycemia mice.
The specific embodiment
Below further specify specific implementation process of the present invention by embodiment
Embodiment 1
The Folium Mori pulverizing medicinal materials becomes coarse powder 10kg, the alcohol reflux with 70% 2 times, 150kg first, 2 80kg; Ethanol extract filter to merge, and 60 ℃ of decompression recycling ethanols, is concentrated to relative density and equals 1.15/80 ℃ (not had the alcohol flavor), and weighing gets 1.93kg, add water 8kg, boil and put coldly after 30 minutes, add hydrochloric acid and regulate pH=3～2, put after 24 to 72 hours sucking filtration and collect insoluble matter and filtrate respectively.80 ℃ of insoluble matters decompression oven dry weigh 682g, pulverize standby.
Filtrate is fully adsorbed with the polyamide resin column of handling well (the heavy 5kg of resin) earlier, and the solution after polyamide is handled fully adsorbs with cation exchange resin column (the heavy 10kg of resin).The polyamide washing of absorption filtrate is clean, the vexed system of ammonia (added alcohol first and is 40kg for 3 times with 80% alcohol reflux after 4 hours, be 25kg 2 times and 3 times), alcohol extract filters and merges, glacial acetic acid is regulated pH=6.5～7, decompression recycling ethanol, reduction vaporization is condensed into relative density greater than 1.30/80 ℃ extractum, 80 ℃ of decompression oven dry weigh 218g, pulverize standby.
The cation exchange resin of adsorbent solution fully adsorbs, washing is clean, the vexed system of ammonia (added alcohol first and is 80kg for 2 times with 80% alcohol reflux after 4 hours, 2 50kg), alcohol extract filters and merges, decompression recycling ethanol, reduction vaporization is condensed into relative density greater than 1.30/80 ℃ extractum, 80 ℃ of decompression oven dry weigh 413g, pulverize standby.
80 ℃ of bakings of Folium Mori coarse powder after the alcohol extraction remove remaining alcoholic solution, extract 2 times with water boil, add the 150kg that water is medical material weight first, 2 times is 100kg, and extracting liquid filtering merges, reduction vaporization is concentrated to relative density greater than 1.20/80 ℃, add and contain the pure alcoholic solution of measuring greater than 85%, regulator solution contains the alcohol amount 70%, stirs, place 72 hours after-filtration collecting precipitation things, 80 ℃ of decompression oven dry weigh 351g, pulverize standby.
Each material powder that aforementioned extraction is made equivalent in proportion increases progressively mix homogeneously promptly.
Embodiment 2
Capsule
The dried cream 200g of [prescription] Folium Mori medical starch 98g magnesium stearate 2g
Make 1000 of hard capsules altogether.
[method for making] makes fine powder with mulberry extract, and packing into by equivalent increment method and medical starch, the abundant mix homogeneously of magnesium stearate gets final product in the hard capsule case.
Embodiment 3
Tablet
The dried cream 200g of [prescription] Folium Mori medical starch 100g magnesium stearate 2g
Make 1000 coated tablets altogether.
[method for making] makes fine powder with mulberry extract, by equivalent increment method and the abundant mix homogeneously of medical starch, neutralizes the sheet granule with 60% ethanol, and dry, granulate add the abundant mix homogeneously of magnesium stearate, and tabletting, sugar coating get final product.
Embodiment 4
Granule
The dried cream 1000g of [prescription] Folium Mori dextrin 980g sweeting agent and other adjuvant 20g
Make 100 bags altogether.
[method for making] makes fine powder with mulberry extract, and by abundant mix homogeneously such as equivalent increment method and dextrin, sweeting agent, with 60% ethanol system granule, drying, granulate are sub-packed in the appropriate containers and get final product.
Claims (7)
1. the Folium Mori extract with antiinflammatory, hypoglycemic activity is characterized in that this Folium Mori extract contains effective ingredient such as Folium Mori alkaloid, flavone, polysaccharide.
2. the preparation method of the Folium Mori extract of claim 1 is characterized in that through following steps:
2.1 the Folium Mori pulverizing medicinal materials is become coarse powder, aqueous methanol with 10% to 95% or ethanol extraction;
2.2 extracting liquid filtering, concentrate, add water, add acid and place after-filtration;
2.3 the insoluble matter oven drying at low temperature of filter collecting, pulverize standbyly, after filtrate is crossed polyamide earlier and fully adsorbed, cross cation exchange resin and fully adsorb,
2.4 the polyamide of absorption filtrate washing is clean, with 10% to 95% aqueous methanol or ethanol extraction, it is closely neutral that extracting solution is regulated pH with acetic acid, reclaims alcoholic solvent after 4 hours for the vexed system of ammonia, concentrating under reduced pressure becomes the pulverizing of extractum after drying standby;
2.5 the ion exchange resin of absorption filtrate washing is clean, with 10% to 95% aqueous methanol or ethanol extraction, extracting solution reclaims alcoholic solvent to the vexed system of ammonia after 4 hours, concentrating under reduced pressure becomes the pulverizing of extractum after drying standby;
2.6 the Folium Mori coarse powder after the alcohol extraction is removed remaining alcoholic solution, use water extraction, extracting liquid filtering merges, reduction vaporization is concentrated to relative density greater than 1.20/80 ℃, add and to contain the alcohol amount and contain the alcohol amount greater than 85% alcoholic solution regulator solution and stir 50%～70%, place 24～72 hours after-filtration collecting precipitation things, oven drying at low temperature is pulverized standby;
2.7 the abundant mix homogeneously of material powder with 2.3,2.4,2.5 and 2.6 promptly.
3. claim 2 described preparation methoies with Folium Mori extract of antiinflammatory, hypoglycemic activity, it is characterized in that: best production technology is as follows:
Alcohol reflux with 70% 2 times, 15 times first, 2 times 8 times; Ethanol extract filter to merge, and behind the decompression recycling ethanol, is concentrated to relative density and equals 1.15/80 ℃, adds 4 times of water gagings, boils and put coldly after 30 minutes, adds hydrochloric acid adjusting pH=3～2, puts after 24 to 72 hours sucking filtration and collects insoluble matter and filtrate respectively.80 ℃ of decompressions of insoluble matter are dried, pulverize standby.
Filtrate is fully adsorbed with the polyamide of handling well earlier, and the solution after polyamide is handled fully adsorbs with cation exchange resin.The polyamide washing of absorption filtrate is clean, the vexed system of ammonia (added alcohol first and is 8 times of resin weight in wet base points for 3 times with 80% alcohol reflux after 4 hours, be 5 times 2 times and 3 times), alcohol extract filters and merges, glacial acetic acid is regulated pH=6.5～7, decompression recycling ethanol, reduction vaporization is condensed into relative density greater than 1.30/80 ℃ extractum, 80 ℃ of decompression oven dry, pulverize standby.
The cation exchange resin of adsorbent solution fully adsorbs, washing is clean, the vexed system of ammonia (added alcohol first and is 8 times of resin weight in wet base points for 2 times with 80% alcohol reflux after 4 hours, 2 times is 5 times), alcohol extract filters and merges, decompression recycling ethanol, reduction vaporization is condensed into relative density greater than 1.30/80 ℃ extractum, 80 ℃ of decompression oven dry, pulverize standby.
80 ℃ of bakings of Folium Mori coarse powder after the alcohol extraction remove remaining alcoholic solution, extract 2 times with water boil, add water first and be 15 times of medical material weight, 2 times is 10 times, and extracting liquid filtering merges, reduction vaporization is concentrated to relative density greater than 1.20/80 ℃, add and contain the pure alcoholic solution of measuring greater than 85%, regulator solution contains the alcohol amount 70%, stirs, place 72 hours after-filtration collecting precipitation things, 80 ℃ of decompressions are dried, pulverize standby.
Each material powder that aforementioned extraction is made equivalent in proportion increases progressively mix homogeneously promptly.
4. claim 2 described preparation methoies with Folium Mori extract of antiinflammatory, hypoglycemic activity is characterized in that: the extraction of medical material can be adopted dynamic reflux, extract,, boils extraction, percolation extracts static immersion, microwave extraction etc.Extract the extract dry mode and can adopt modes such as room temperature oven dry, decompression oven dry, spray drying.
5. claim 2 described Folium Mori extracts with antiinflammatory, hypoglycemic activity, it is characterized in that: can add suitable adjuvant and make any dosage form, as (not only comprising): sugar coated tablet, film coated tablet, enteric coated tablet, buccal tablet, effervescent tablet, hard capsule, soft capsule, pill, drop pill, granule, medicinal tea, powder, unguentum, sublimed preparation, suspensoid, solution, injection, suppository, plaster, ointment, cream, spray, drop, patch etc. are as medicine, the utilization of healthy Related product.The preferential dosage form of selecting of the present invention is an oral formulations, as tablet, capsule, granule, mixture etc.
6. claim 2 described Folium Mori extract and preparations thereof with antiinflammatory, hypoglycemic activity, it is characterized in that: the adjuvant of preparation preparation is the adjuvant that country and industry standard and rules allow adding, such as (not only comprising): starch, sucrose, dextrin, mannitol, glycerol, silicon derivative, magnesium stearate, Pulvis Talci etc.
7. claim 2 described Folium Mori extracts with antiinflammatory, hypoglycemic activity is characterized in that: can be used for prevention, treatment and the rehabilitation of diseases such as inflammation that non-infective pathogen body infectious disease causes, diabetes; Also can with the medication combined application of resisting pathogenic microbes, the inflammation that treatment infective pathogen body infectious disease causes.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229862A (en) * | 2010-10-15 | 2011-11-02 | 华芳烟用香料有限公司 | Preparation of mulberry leaf volatile oil and application thereof in tobacco flavoring |
| CN104940293A (en) * | 2015-06-15 | 2015-09-30 | 塔里木大学 | Method for extracting alkaloid, polysaccharide and flavone from mulberry leaves |
| CN113133307A (en) * | 2019-10-31 | 2021-07-16 | 马克一创新中心有限公司 | Composition comprising mulberry extract and method for producing the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1579445A (en) * | 2003-08-05 | 2005-02-16 | 上海华源生命科学研究开发有限公司 | Effective-component extract of Foliun-Mori and its preparation method |
| CN1742803A (en) * | 2005-08-11 | 2006-03-08 | 原爱红 | Chinese medicine extract with function of reducing blood-sugar and preparing method |
| CN1981814A (en) * | 2005-12-15 | 2007-06-20 | 天津中新药业集团股份有限公司 | Use and production for mulberry leaf in treatment of diabetes |
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2010
- 2010-03-12 CN CN 201010122597 patent/CN101780147A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1579445A (en) * | 2003-08-05 | 2005-02-16 | 上海华源生命科学研究开发有限公司 | Effective-component extract of Foliun-Mori and its preparation method |
| CN1742803A (en) * | 2005-08-11 | 2006-03-08 | 原爱红 | Chinese medicine extract with function of reducing blood-sugar and preparing method |
| CN1981814A (en) * | 2005-12-15 | 2007-06-20 | 天津中新药业集团股份有限公司 | Use and production for mulberry leaf in treatment of diabetes |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229862A (en) * | 2010-10-15 | 2011-11-02 | 华芳烟用香料有限公司 | Preparation of mulberry leaf volatile oil and application thereof in tobacco flavoring |
| CN102229862B (en) * | 2010-10-15 | 2013-07-31 | 华芳烟用香料有限公司 | Preparation of mulberry leaf volatile oil and application thereof in tobacco flavoring |
| CN104940293A (en) * | 2015-06-15 | 2015-09-30 | 塔里木大学 | Method for extracting alkaloid, polysaccharide and flavone from mulberry leaves |
| CN104940293B (en) * | 2015-06-15 | 2018-01-09 | 塔里木大学 | A kind of method that alkaloid, polysaccharide and flavones are extracted from mulberry leaf |
| CN113133307A (en) * | 2019-10-31 | 2021-07-16 | 马克一创新中心有限公司 | Composition comprising mulberry extract and method for producing the same |
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