CN1017446B

CN1017446B - Brewing method for beer containing no carcinogen

Info

Publication number: CN1017446B
Application number: CN 90100756
Authority: CN
Inventors: 孔兆钦; 孔宪一
Original assignee: Individual
Current assignee: Individual
Priority date: 1990-02-19
Filing date: 1990-02-19
Publication date: 1992-07-15
Also published as: CN1054097A

Abstract

The present invention relates to a method for brewing beer containing no carcinogenic substances. The known beer is brewed by taking barley malt as a main raw material. The beer not only has high material cost, but also contains carcinogenic substances, such as dimethyl nitrosamine and aflatoxin B1. The frequent drinking of the beer is bad for human health. In the method, corn, wheat, long rice and low-grade barley are used as raw materials, beer syrup is prepared by additionally adding enzymes, and the beer is brewed by fermenting the mixture. The adoption of the method for brewing beer has the advantages of low cost, simple technology, and completely eradication of the carcinogenic substances in the beer.

Description

Brewing method for beer containing no carcinogen

A kind of brewing method that does not contain carcinogenic beer belongs to the application method class of the brewing method and the enzyme of beer.

Beer mainly is to be main raw material with the Fructus Hordei Germinatus now, forms through saccharification and fermentation.Saccharifying enzyme in the saccharifying is provided by Fructus Hordei Germinatus, as α-Dian Fenmei, and beta-amylase etc.Along with chemistry, the development of medical science, the appearance of rigorous analysis instrument and application, will adopt Fructus Hordei Germinatus is that all inherent shortcoming of main raw material beer brewing discloses out.The one, waste is ploughed and is lacked economic benefit, and barley is a low-yielding crop, all is unaccommodated as grain or cash crop.Especially China's barley production is few, and wherein major part is a feed barley, and the output of the special-purpose barley of beer just still less; The 2nd, run counter to biochemical basis, malt drying is a kind of " waste " to a certain extent, discharges a large amount of moisture content during oven dry, heavily adds a large amount of water during saccharification again.Require Fructus Hordei Germinatus to have enough enzyme activities during saccharification so that increasing supplementary product consumption reduces cost, and in malt drying, make a large amount of enzyme deactivations; The 3rd, contain carcinogenic substance-N-nitrosodimethylamine in the Fructus Hordei Germinatus, and contain another carcinogenic substance-flavacin B in the beer of brewageing with this method ₁, these two kinds of materials all belong to the intensive carcinogens, therefore, drink Fructus Hordei Germinatus beer in large quantities and will bring harm to human body.Disclose among the US-A-3081172 a kind of usefulness not germinated ceral add the method that the liver starch saccharifying enzyme is produced syrup for beer, the weak point of this method is: 1, the source of enzyme is an animal body, and consumption is big, can not reach mass industrialized production.2, with cereal be raw material and do not remove cot, the embryo navel will be introduced the local flavor that a large amount of fat influences beer.3, long reaction time, technical process complexity, plant factor is low, beer cost height.The object of the present invention is to provide a kind of is raw material without Fructus Hordei Germinatus, can eradicate the brewing method of the beer of the carcinogenic substance in the beer.

The object of the present invention is achieved like this, and it is to be raw material with cereal, and as being raw material with corn, rice, Chinese sorghum, millet, barley, wheat, long-grained nonglutinous rice or their mixture, additional enzyme is made syrup for beer, then through fermentative processing, brewages into beer.The raw material that the present invention selects for use can be a corn fine powder of removing the embryo navel, remove rice (rice) fine powder of cot, remove the Chinese sorghum fine powder of cot, remove the millet fine powder of cot, remove the barley corn fine powder of cot, remove the wheat fine powder of cot, remove a kind of in the long-grained nonglutinous rice fine powder of cot or their mixture.The used enzyme of the present invention is two groups of mixed enzyme, and it comprises microbial amylase, microbial protease, plant protease, maltogenic amylase of plant and microbial glucoamylase.The addition of enzyme is that per 100 parts of raw materials add first group of mixed enzyme 0.1-4 weight part, second group of mixed enzyme 0.1-3 weight part.

First group of mixed enzyme comprises microbial amylase, the plant protease bio protease, second group of mixed enzyme comprises microbial glucoamylase or maltogenic amylase of plant, plant protease or bio protease, wherein said enzyme comprises: microbial amylase, as AS7658 amylase (commercial goods title) etc., plant protease, as bromeline, papain (being commercially available) etc., microbial protease is as 1398 proteolytic enzyme, 166 proteolytic enzyme (being the commercial goods title) etc., microbial glucoamylase, as 3942 saccharifying enzyme (commercial goods title) etc., maltogenic amylase of plant is as β saccharifying enzyme (commercially available) etc.Above-mentioned said two groups of mixed enzyme are respectively: in first group of mixed enzyme, microbial amylase accounts for 40-80%(weight), plant protease or microbial protease or their mixture account for 60-20%(weight).In second group of mixed enzyme, microbial glucoamylase or maltogenic amylase of plant or their mixture account for 20-60%(weight), plant protease or microbial protease or their mixture account for 40-80%(weight).

Implementation step of the present invention is: drop into brewing water 720-840 kilogram in brew kettle, under agitation add chemical pure calcium chloride 450-540 gram, remove embryo navel corn fine powder 175-180 kilogram, add first group of mixed enzyme 800-1800 gram thereupon, continuing to stir thoroughly dissolves enzyme, after corn fine powder is soaked into fully, adjust pH 6.1-7.8 is heated to 75-95 ℃ of insulation 20-40 minute with solution, inspect with iodine liquid around here, when treating that solution is yellowish brown, lower the temperature 60 ℃ and drop into second group of mixed enzyme 1200-1500 gram again after down, be incubated 120-140 minute down at 40-60 ℃, after intensification is boiled 3-15 minute then, reduce to 80-90 ℃ and remove corn groove slag with the logical machine of sheet frame filter, again syrup is squeezed in the boiling pot, add fresh hops 1-1.8 kilogram, adjust pH 5.2-5.4, boiled 40-60 minute, divide and remove the hops slag, syrup is pumped in the settling bath cool off, reduce to 7-9 ℃ through water cooler again, the enzyme mother of the 0.6-1.5% of ferment pond adding syrup total amount is fermented before pumping into, the product temperature keeps 8-12 ℃, ferment after changing in 4-7 days, constant temperature is at 2-3 ℃, through 12-14 days, can make and do not contain carcinogens beer.

The invention has the advantages that:

1. be raw material with ablastous corn, barley, wheat long-grained nonglutinous rice, additional enzyme is produced syrup for beer, and without Fructus Hordei Germinatus, has eradicated generation carcinogenic substance N-nitrosodimethylamine and flavacin B fully ₁Possibility, the beer of brewageing with method of the present invention can guarantee N-nitrosodimethylamine and flavacin B ₁Content is zero.Therefore adopt the beer of method brew of the present invention to help HUMAN HEALTH.And therefore the precursor carotene that contains anticancer specific factor gsh and cancer-resisting substance vitamin A in the corn be the not only not carcinogenic effect that can also play anti-cancer of the beer of raw material brew with it.

2. corn, wheat long-grained nonglutinous rice all are high-yield crops in China, and the used barley of present method can be the feed barley of low-quality, so the present invention can make the raw materials cost of beer reduce by 1/3.

3. the present invention has selected domestic commercial, the microbial amylase, microbial protease and the maltogenic amylase of plant that are easy to get for use, is convenient to carry out large industrialized production.

4. add enzymatic protective reagent, can improve the vigor of enzyme greatly, reduced the consumption of enzyme, improved the production unit utilization ratio, reduced energy consumption.

5. adopt the beer of method brew of the present invention, except that not containing N-nitrosodimethylamine, AFB ₁Outside the carcinogens, its physical and chemical index, sense index meet national GB4729-85 top grade beer quality standard fully, and sanitary index meets national GB2758-81 standard.

For example method of the present invention is elaborated below:

Example 1, in brew kettle, drop into 720 kilograms of brewing waters, under agitation add chemical pure calcium chloride 500 grams, remove 180 kilograms of the corn fine powders of embryo navel, add first group of mixed enzyme, 2000 grams thereupon, continuing to stir thoroughly dissolves enzyme, after corn fine powder was soaked into fully, adjust pH 6.4 was heated to 89 ℃ of insulations 38 minutes with solution, inspect with iodine liquid around here, when treating that solution is yellowish brown, be cooled to 49 ℃ rapidly and drop into second group of mixed enzyme, 1500 grams again, be incubated 140 minutes down at 51 ℃, after intensification is boiled 3 minutes again, reduce to 90 ℃ and remove corn groove slag with flame filter press, syrup is squeezed in the boiling pot again, added 1.2 kilograms in fresh hops, adjust pH 5.3 boiled 40 minutes, divide and remove the hops slag, syrup pumped in the settling bath cool off, reduce to 8 ℃ through the thin plate water cooler, the ferment pond adds yeast and ferments for 8 kilograms before pumping into, the product temperature keeps 9 ℃, ferment after changing in 5 days, constant temperature were promptly brewageed into 1 ton through 14 days and are not contained carcinogens beer at 2 ℃.

Example 2, composition of raw materials: remove 100 kilograms of the wheat fine powders of cot, 100 kilograms of the barley fine powders of Ex-all cot add first group of mixed enzyme, 1700 grams, second group of mixed enzyme 1100 gram, and other processing method is same with example 1.

Example 3, composition of raw materials: remove 170 kilograms of embryo navel corn fine powders, remove 20 kilograms of the barley corn fine powders of cot, add first group of mixed enzyme, 1800 grams, second group of mixed enzyme 1100 gram, other processing method is same with example 1.

Claims

1, a kind of brewing method that does not contain carcinogenic beer, it comprises produces syrup and fermenting process, it is characterized in that, with cereal is raw material, add first group of mixed enzyme 0.1-4 weight part in per 100 weight part raw materials, the chloride enzyme protecting agent of second group of mixed enzyme 0.1-3 weight part and 0.12-0.3 weight part, wherein two groups of mixed enzyme are respectively: first group of mixed enzyme comprises, microbial amylase, plant protease or microbial protease, second group of mixed enzyme comprises, microbial glucoamylase or maltogenic amylase of plant, plant protease or microbial protease, its brewing method is, earlier with water, material grain, calcium chloride and first group of mixed enzyme are mixed, regulate pH value 6.1-7.8, with this mixture heating up to 75-95 ℃ the insulation 20-40 minute, inspect with iodine liquid around here, when treating that solution is yellowish brown, cooling adds second group of mixed enzyme below 60 ℃, be incubated 120-140 minute down at 40-60 ℃, after intensification is boiled 3-15 minute, reduce to 80-90 ℃, filter and remove residue is squeezed into this syrup in the boiling pot again, add fresh hops, adjust pH 5.2-5.4 boiled 40-60 minute, divided and removed the hops slag, syrup pumped in the settling bath cool off, reduce to 7-9 ℃ through water cooler again, 0.6-1.5% (weight) yeast of ferment pond adding syrup total amount ferments before pumping into, and the product temperature keeps 8-12 ℃, after 4-7 days, change the back ferment over to, constant temperature is at 2-3 ℃, through 12-14 days, promptly makes and do not contain carcinogens beer; A kind of brewing method that does not contain carcinogenic beer.

2, according to the brewing method of claim 1, in wherein said first group of mixed enzyme, microbial amylase accounts for 40-80%(weight), plant protease or microbial protease or their mixture account for 60-20%(weight).

3, according to the brewing method of claim 1, in wherein said second group of mixed enzyme, microbial glucoamylase or maltogenic amylase of plant or their mixture account for 20-60%(weight), plant protease or microbial protease or their mixture account for 40-80%(weight).

4, according to the brewing method of claim 1, wherein said cereal can be corn, rice, Chinese sorghum, millet, barley, wheat and Zhou rice or their mixture.