CN101700027B - Synergistic bactericidal composition and application - Google Patents

Abstract

The invention belongs to the technical field of pesticide, in particular to a bactericidal composition containing polyoxin and cyazofamid which are compounded and an application. The synergistic bactericidal composition adopts the polyoxin and the cyazofamid to be compounded to act a synergistic action, is used for preventing and treating various diseases on crops, such as melons, fruit, vegetables and the like, has the advantages of wide application range, low cost, good effect, resistibility delaying of pathogenic bacteria, high bactericidal activity and the like and especially has very good preventing and treating effect to downy mildew and blight.

Description

A kind of Synergistic bactericidal composition and application

Technical field

The invention belongs to the technical field of agricultural chemicals, be specifically related to a kind of contain polyoxin and cyazofamid composite bactericidal composition and application.

Background technology

The downy mildew that is produced by the Oomycete fungal attack, epidemic disease are the multiple diseases of protection ground vegetables and other crop (such as grape, strawberry, potato etc.); have infectivity, infect soon; develop the characteristics such as rapid, wayward, often cause a large amount of underproduction even total crop failure to crops.In recent years along with the increasing of China fruit tree and growing vegetables area; especially the spread of vegetable protecting field culture technique; so that the incidence of crop pest and occurring degree all are significantly increased; the vegetable disease such as cucumber downy mildew and tomato blight has become the No.1 killer in the protection ground growing vegetables; often cause mashed leaf, decayed fruit, and even complete stool is dead.The frequent occurrence of disease so that the frequency of utilization of bactericide constantly increase, and the dispenser of high frequency time makes germ produce more and more stronger drug resistance, thereby cause vicious circle, conventional dose such as carbendazim, aliette etc. can not effectively be controlled such disease, and some former efficient kinds such as metalaxyl, Propamocarb etc. are to the control efficiency of downy mildew also worse and worse.

The composition of different cultivars carries out composite, is the common way of preventing and treating the resistance germ, according to practical application effect, judge certain composite be synergy, addition or antagonism.Composite effect is well filled a prescription, owing to obviously improved actual control efficiency, reduced the usage amount of agricultural chemicals, thereby greatly delayed the drug-fast generation of germ, and be the important means of integrated control disease.

Cyazofamid (Cyazofamid) chemical name 4-chloro-2-cyano group-5-p-methylphenyl-imidazoles-1-N, N-dimethyl methyl acid amides belongs to the imidazoles bactericide.Cyazofamid is the mitochondrial respiratory inhibitor; all vegetative stages of oomycetes all there is effect; the germ that metalaxyl is produced resistance or sensitivity all has activity; and has good prolection; lasting period is long, and resistance of rainwater washing against is to crop, the mankind, Environmental security; but long-term single use can make germ that it is developed immunity to drugs unavoidably.

Polyoxin (Polyoxin) claim again multi-effect mycin, chemical name peptide pyrimidine nucleoside, belong to the broad-spectrum antibiotics series bactericidal agent, can disturb the chitinous biosynthesis of germ cell wall, germ tube is with after mycelia contacts medicament, and cell inclusion is expanded, breaks, overflowed in the part, and can not normal development, also can suppress germ and produce spore and scab expansion, can prevent and treat the multiple diseases such as eliminating vegetable botrytis, downy mildew, epidemic disease, have interior suction conduction.

The long-term separately use of above-mentioned two kinds of medicaments causes germ to develop immunity to drugs easily; bactericidal activity is reduced greatly; and can not have preferably protection and therapeutic activity concurrently, control efficiency is unsatisfactory, has not yet to see the relevant report of polyoxin and the composite product of cyazofamid.

Summary of the invention

The objective of the invention is provides a kind of Synergistic bactericidal composition and application for the problems referred to above, said composition adopts polyoxin and cyazofamid to carry out composite, play synergistic function, be used for preventing and treating the multiple diseases on the crops such as melon and fruit, vegetables, applied widely, cost is low, effective, especially downy mildew, epidemic disease there are good control efficiency, have the germ of delaying resistance and bactericidal activity advantages of higher.

Among the present invention, the weight that the cyazofamid of mentioning, the weight of polyoxin and Fungicidal active substance are total etc. all refer to the weight of rolling over after hundred, i.e. the weight of active principle.

Synergistic bactericidal composition of the present invention contains Fungicidal active substance polyoxin and cyazofamid.

Wherein, the weight ratio of described Fungicidal active substance polyoxin and cyazofamid is polyoxin: cyazofamid=1: 1-1: 100.

As preferably, the weight ratio of described Fungicidal active substance polyoxin and cyazofamid is polyoxin: cyazofamid=1: 5～1: 20.

In the said composition, the total weight percentage of described Fungicidal active substance is 1%～90%.

Composition of the present invention adds auxiliary agent and excipient, makes according to the method that those skilled in the art were familiar with to be fit to the upper formulation of using of agricultural, and preferably formulation is wetting powder, suspending agent and water dispersible granules.

Synergistic bactericidal composition of the present invention is used for the disease on control melon and fruit, the vegetable crop, can prevent and treat the multiple diseases on melon and fruit, the vegetable crop, especially downy mildew, epidemic disease is had good control efficiency.

The present invention carries out polyoxin and cyazofamid bactericide composite, and cyazofamid is the mitochondrial respiratory inhibitor, and polyoxin belongs to the antibiotics bactericide, because the mechanism of action is different between the two, has greatly delayed the drug-fast generation of germ; And complex preparation has higher protection and therapeutic activity concurrently; greatly improve bactericidal effect, had obvious synergistic function, thereby reduced dosage and drug cost; reduced the harmful effect to environment, thereby this complex composition have obvious economic benefit and environmental benefit.In a word, this complex composition is used for preventing and treating the multiple diseases on the crops such as melon and fruit, vegetables, applied widely, cost is low, effective, especially downy mildew, epidemic disease are had good control efficiency, have the germ of delaying resistance and bactericidal activity advantages of higher.

Measure polyoxin and cyazofamid list mixture to the indoor virulence of bacterium of downy mildew of cucumber in indoor employing plate blade method.Test method: place culture dish to carry out moisturizing cucumber leaves and process, dosage by design sprays respectively medicament, inoculate bacterium of downy mildew of cucumber after 24 hours, and moisturizing is cultivated in incubator, 7d " Invest, Then Investigate " preventive effect, calculate control efficiency with disease index, carry out statistical analysis with the DPS data processing software, calculate the EC50 of each medicament, adopt the abundant method calculating co-toxicity coefficient of Sun Yun (CTC) to estimate and use effect with, the CTC value has synergistic effect greater than 120 o'clock mixtures, less than 80 o'clock be antagonism, have summation action in the time of between 80～120.

The EC50*100 of the EC50/B agent of toxicity index TI (B)=standard agent A

The EC50*100 of the EC50/AB of actual toxicity index ATI (AB)=A

Theoretical toxicity index TTI (AB)=percentage+TI (B) * B the percentage in mixture of TI (A) * A in mixture

Co-toxicity coefficient CTC=ATI (AB)/TTI (AB) * 100

Test data is as follows:

Table 1 polyoxin and cyazofamid list mixture are to the toxicity test of bacterium of downy mildew of cucumber

Reagent agent	The active ingredient weight ratio	EC50(mg/L)	Co-toxicity coefficient (CTC)
				Polyoxin		20.86
Cyazofamid		8.72
				Polyoxin: cyazofamid	1∶1	12.32	199.65
Polyoxin: cyazofamid	1∶5	8.18	236.10
				Polyoxin: cyazofamid	1∶10	7.13	258.26
Polyoxin: cyazofamid	1∶20	6.89	260.33
				Polyoxin: cyazofamid	1∶40	8.27	213.91
Polyoxin: cyazofamid	1∶80	9.36	187.67
				Polyoxin: cyazofamid	1∶100	11.46	153.06

By as seen from Table 1, polyoxin and cyazofamid are composite, bacterium of downy mildew of cucumber had good indoor virulence, the CTC value of seven proportionings is all above 120, show that these two bactericide have good synergistic function under this proportioning, can find out also simultaneously that in composition different its synergistic effects along with polyoxin and cyazofamid ratio also are different, mixed ratio synergy between 1: 5～1: 20 is particularly remarkable.

Embodiment

The invention will be further described below in conjunction with embodiment, but the present invention is not limited thereto, and the formulation preparation method among the embodiment is conventional method.

Embodiment 1:9% polyoxin cyazofamid suspending agent comprises following component by weight:

Polyoxin 1.5%

Cyazofamid 7.5%

Alkyl naphthalene formaldehyde condensate sulfonates 1%

Polyoxyethylene nonylphenol ether 1%

Xanthans 0.3%

Benzoic acid 0.1%

Organosilan defoamer 0.3%

Water surplus

With whole mixing of materials, stirring joins sand mill to material and carries out sand milling afterwards, makes material reach 2～5 μ m fineness, makes 9% polyoxin cyazofamid suspending agent.

Embodiment 2:21% polyoxin cyazofamid water dispersible granules comprises following component by weight:

Polyoxin 1%

Cyazofamid 20%

Sodium lignin sulfonate 6%

PO-EO block polyether 4%

Lauryl sodium sulfate 3%

Ammonium sulfate 4%

The precipitated calcium carbonate surplus

The ratio of said components in prescription mixed, become wetting powder through air-flow crushing, add again a certain amount of water mixing extruder grain, obtain 21% polyoxin cyazofamid water dispersible granules after the drying screening.

Embodiment 3:80% polyoxin cyazofamid wetting powder comprises following component by weight:

Polyoxin 1%

Cyazofamid 79%

To methoxyl group fatty acid Sodium p-aminobenzene sulfonat 1%

Sodium methylene bis-naphthalene sulfonate 2%

White carbon 2.5%

The kaolin surplus

Said components is mixed into promoting the circulation of qi stream pulverizes, make 80% polyoxin cyazofamid wetting powder.

Test example: the test of pesticide effectiveness of the mixture control tomato late blight of polyoxin and the different proportionings of cyazofamid

Several mixtures that the embodiment of the invention is obtained carry out the test of pesticide effectiveness, adopt the greenhouse pot culture tomato seedling, measure single mixture of polyoxin and cyazofamid to the control efficiency of tomato late blight.When tomato grows to 3～4 leaves, select the blade of growing way uniformity to adopt manual sprayer to carry out the foliar spray processing, inoculate pathogen after 24 hours, 7d " Invest, Then Investigate " result is cultivated in moisturizing, the results are shown in Table 2.

The mixture of table 2 polyoxin and the different proportionings of cyazofamid is to the control efficiency of tomato late blight

Reagent agent	Dosage (μ g/mL)	Disease index	Control efficiency (%)
				Embodiment 1 product	200	11.94	82.13
Embodiment 2 products	200	12.28	81.62
				Embodiment 3 products	200	13.22	80.21
20% polyoxin suspending agent	200	17.86	73.27
				20% cyazofamid suspending agent	200	16.74	74.94
The clear water contrast		66.82

As can be seen from Table 2, the mixture preparation of polyoxin and cyazofamid can effectively be prevented and treated tomato late blight, and obviously is better than the control efficiency of single dose.

As can be seen from the above embodiments, polyoxin of the present invention and cyazofamid composition are in certain ratio range, has good synergistic function, epidemic disease there is good control efficiency, thereby can reduce the usage amount of medicine, reduce residue of pesticide, reduce the influence degree to environment, play environmental protection, efficient effect.

Claims (5)

1. a Synergistic bactericidal composition contains Fungicidal active substance polyoxin and cyazofamid; The weight ratio of described Fungicidal active substance polyoxin and cyazofamid is polyoxin: cyazofamid=1:5～1:20.

2. Synergistic bactericidal composition according to claim 1 is characterized in that, the total weight percentage of described Fungicidal active substance is 1%～90%.

3. Synergistic bactericidal composition according to claim 2, the said composition formulation is wetting powder, suspending agent and water dispersible granules.

4. according to Synergistic bactericidal composition claimed in claim 1, be used for the multiple diseases on control melon and fruit, the vegetable crop.

5. according to Synergistic bactericidal composition claimed in claim 1, be used for preventing and treating downy mildew, epidemic disease.