CN101664533A - Chinese medicament for treating acute and chronic mammary hyperplasia and preparation method - Google Patents

Chinese medicament for treating acute and chronic mammary hyperplasia and preparation method Download PDF

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CN101664533A
CN101664533A CN 200810051138 CN200810051138A CN101664533A CN 101664533 A CN101664533 A CN 101664533A CN 200810051138 CN200810051138 CN 200810051138 CN 200810051138 A CN200810051138 A CN 200810051138A CN 101664533 A CN101664533 A CN 101664533A
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portions
radix
chronic
mixture
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CN101664533B (en
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臧育增
高陆
康亚娟
李凌
白冰
隋娜
高红梅
岳雪玲
高爽
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Tongyao Pharmaceutical Group Corporation
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李艳华
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Abstract

The invention discloses a Chinese medicament for treating acute and chronic mammary hyperplasia and a preparation method. The Chinese medicament consists of the following raw materials in portion by weight: 4 to 20 portions of dandelion, 2 to 10 portions of Chinese thorowax root, 2 to 10 portions of sauted green tangerine peel, 2 to 10 portions of fritillary bulb, 2 to 10 portions of turmeric root-tuber, 3 to 15 portions of snakegourd fruit, 2 to 10 portions of Chinese angelica, and 2 to 10 portions of sauted nutgrass galingale rhizome. The preparation method comprises the following steps: extracting the fritillary bulb and the Chinese angelica by using ethanol, and concentrating the ethanol extract to form an extract; extracting the dandelion, a part of green tangerine peel, the snakegourd fruit, the turmeric root-tuber and the nutgrass galingale rhizome by using water, and concentrating the water extract to form an extract; and mixing the two extracts, then evenly mixing the mixed extract and a part of green tangerine peel which is ground into fine powder, reducing the pressure and drying the mixture, crushing the mixture and adding proper amount of accessories into the mixture,evenly mixing and pelletizing the mixture, adding proper amount of dried starch and lubricant into the mixture, and evenly mixing the mixture to obtain the Chinese medicament. The Chinese medicament used for treating acute and chronic mammary hyperplasia diseases has obvious inhibition or treatment effect on the mammary hyperplasia caused by estrogen reduction and endocrine dyscrasia, and has obvious analgesia and anti-inflammatory effects at the same time.

Description

A kind of Chinese medicine and preparation method for the treatment of acute and chronic mammary hyperplasia
Technical field
The invention belongs to the new Chinese medicine technical field, be specifically related to a kind of Chinese medicine preparation for the treatment of the acute and chronic mammary hyperplasia disease and preparation method thereof.
Technical background
Cyclomastopathy belongs to the category of the traditional Chinese medical science " nodules of the breast ".Be young women commonly encountered diseases, frequently-occurring disease, account for more than 70% of mastopathy.Primary disease is comparatively common, and its sickness rate of domestic report and is mainly in 30~50 years old women about 10%, and the man also has generation.Modern medicine thinks that it is relevant with endocrine dysfunction, is principal character with mastalgia and lump in breast.Chinese medicine thinks that the etiology and pathogenesis of primary disease is a depression of liver-QI, the expectorant blood coagulation stasis of blood, Chong and Ren channel disorder; Wherein Chong and Ren channel disorder is for falling ill originally, and depression of liver-QI, the expectorant blood coagulation stasis of blood are the mark of morbidity, and sick position is at liver spleen kidney, and characteristic of disease is a deficiency in origin and excess in superficiality.Zhang Xiantao, Guo Sihong, Su Ying think that stagnation of QI due to depression of the liver is the main cause that nodules of the breast takes place, Xu Lvhe according to diseases such as primary disease distending pain of the breast caking, belch uncomfortable in chest in being in a very depressed state, increasing the weight of during menstrual onset, think that the primary disease pathogenesis is liver, liver influences each other depression of liver-QI, Chong and Ren channel disorder with dash appointing, through water delegation, irritability must be relaxed, Gu all diseases all subtract, so on the method for treatment based on dispersing the stagnated live-QI to relieve the stagnation of QI.Contemporary well-known doctor Gu Baihua basis clinical experience for many years proposes Chong and Ren channel disorder and the common morbific viewpoint of stagnation of QI due to depression of the liver, is extensively approved, clearly proposes " control the first treating the liver of addiction, the controlled atmosphere addiction is clear certainly " in treatment.Ni Dajun also obtains good effect with transferring liver ruling by law to treat the Chong and Ren channel disorder type mastoproliferation disease, proved the reasonability of controlling cyclomastopathy from the liver opinion, Gong Yunbin also thinks that the generation of nodules of the breast and stagnation of QI due to depression of the liver are in close relations, because of can causing blood stasis expectorant, the stagnation of QI coagulates, and the two can become paathogenic factor, and the stagnation of QI is increased the weight of.Lin Yi, Xu Guisheng think that then Chong and Ren channel disorder is the basic of nodules of the breast morbidity, and the kidney invigorating accent that adopt dash the method for appointing more in the treatment, have obtained satisfactory effect clinically.
Show the hundreds of thousands women there is every year because of suffering from this sick excision that is forced to undergo surgery according to hygiene department's statistics.Mastopathy is not distinguished by lump, if benign, adopt orally taken for curing, can eliminate at short notice fully; If virulent, even if the caking of grain of rice size is wanted untimely treatment, also can bring out canceration, the women got cyclomastopathy, mammary gland fibroma thus, and mastopathy such as lump in breast must in time be checked treatment.
Though at present clinical the chemotherapy hyperplasia of mammary glands is arranged, certain therapeutical effect is also arranged, toxic and side effects is relatively large.Though some compound Chinese medicinal preparation treatment hyperplasia of mammary glands are also arranged, and curative effect is not really desirable.For example RUKANG PIAN is a monarch drug with the Radix Astragali, is aided with 17 flavor Chinese medicines such as Concha Ostreae, Spica Prunellae, Radix Salviae Miltiorrhizae, Radix Scrophulariae, Bulbus Fritillariae Thunbergii and forms.Its production technology is that 4 kinds of compositions are pulverized, and all the other 13 kinds of flavour of a drug decoct with water secondary, and decocting liquid ethanol precipitation is removed macromolecular substances (polysaccharide etc.); Modern pharmacology studies show that the polysaccharide component in the Radix Astragali, the Bulbus Fritillariae Thunbergii has higher physiological activities, does not reach the purpose that effective ingredient is all kept.
Summary of the invention
The object of the present invention is to provide a kind of Chinese medicine for the treatment of acute and chronic mammary hyperplasia, the cyclomastopathy that estrogen decrease and endocrine disturbance are caused all has obvious suppression or therapeutical effect, has tangible antiinflammatory, analgesic activity simultaneously.
Another object of the present invention is to provide a kind of preparation method for the treatment of the Chinese medicine preparation of acute and chronic mammary hyperplasia disease.
The present invention is made up of the Chinese crude drug of following parts by weight ratio: 4~20 parts of Herba Taraxacis, 2~10 parts of Radix Bupleuri, 2~10 parts of parch skins, 2~10 parts of Bulbus Fritillariae Thunbergiis, 2~10 parts of Radix Curcumaes, 3~15 parts of Fructus Trichosanthis, 2~10 parts of Radix Angelicae Sinensis, 2~10 parts of stir-fry Rhizoma Cyperis.
Best medical material ratio of weight and number of the present invention is: 12 parts of Herba Taraxacis, 6 parts of Radix Bupleuri, 6 parts of parch skins, 6 parts of Bulbus Fritillariae Thunbergiis, 6 parts of Radix Curcumaes, 9 parts of Fructus Trichosanthis, 6 parts of Radix Angelicae Sinensis, 6 parts of stir-fry Rhizoma Cyperis.
Preparation method of Chinese medicine of the present invention is made up of following steps: a, get the raw materials ready: take by weighing raw materials of traditional Chinese medicinal materials according to above-mentioned consumption, each Chinese crude drug cleaned dry, described share is standby by choosing, with Pericarpium Citri Reticulatae Viride, Rhizoma Cyperi parch, pulverize about half of Pericarpium Citri Reticulatae Viride, it is standby to cross 100 mesh sieves; B, ethanol extraction: the Radix Bupleuri of described weight quota, Bulbus Fritillariae Thunbergii, Radix Angelicae Sinensis are added the alcohol reflux twice of 6-12 times of weight, concentration 40-90%, filter then, the extractum of relative density 1.10-1.40 with filtrate recycling ethanol and when being concentrated into 60 degrees centigrade; C, water extraction: half Pericarpium Citri Reticulatae Viride, Herba Taraxaci, Radix Curcumae, Fructus Trichosanthis, Rhizoma Cyperi that pulverizing is remaining, decoct with water three times, each amount of water is 6-12 times, decocts 1-4 hour at every turn, filter the extractum of relative density 1.10-1.40 when filtrate is concentrated into 60 degrees centigrade then; D, combination drying: with two kinds of extractum of gained and Pericarpium Citri Reticulatae Viride fine powder mixing among step b and the step c, in 50-80 degree centigrade of drying under reduced pressure, pulverize the back and add the appropriate amount of auxiliary materials mixed pelletization, drying adds appropriate amount of auxiliary materials and lubricant.
The dosage form of the pharmaceutical composition finished product preparation of treatment dysmenorrhea disease of the present invention is acceptable any dosage form of routine techniques method preparation on the pharmaceutics, as granule, pill, capsule, soft capsule, tablet, mixture, oral liquid etc.
Medicine of the present invention is oral medicine, and consumption is a 10-14 gram, 3 times on the one.
Technical scheme of the present invention is based on understanding and the Therapeutic Principle of motherland's medical science to the acute and chronic mammary hyperplasia disease pathogenesis, with reference to modern pharmacological research achievement, according to depressed liver-energy dispersing and QI regulating, logical transfer to dash appoint, blood circulation promoting and blood stasis dispelling, the disappear principles of formulating prescriptions of addiction of eliminating stagnation form.By adopting Herba Taraxaci, Radix Bupleuri is monarch drug, the soothing the liver heat clearing away of tool, the disappear effect of addiction of eliminating stagnation; Fructus Trichosanthis, Bulbus Fritillariae Thunbergii, Pericarpium Citri Reticulatae Viride, Radix Curcumae are ministerial drug, and it is auxilliary then removing heat-phlegm that Herba Taraxaci, Radix Bupleuri get Fructus Trichosanthis, Bulbus Fritillariae Thunbergii, the disappear Li Youhong of addiction of eliminating stagnation; Pericarpium Citri Reticulatae Viride, Radix Curcumae are aided then dispersing the stagnated live-QI to relieve the stagnation of QI, the merit benefit of the blood stasis dispelling of circulation of qi promoting is clear; Radix Angelicae Sinensis, Rhizoma Cyperi one blood of supervising the cooking, a main circulation of qi promoting, the two blood of regulating the flow of vital energy is transferred to dash and is appointed and be adjuvant.The all medicines in full side are pulled together, long memorial liver and qi altogether, logical transfer to dash appoint blood circulation promoting and blood stasis dispelling, the disappear merit of addiction of eliminating stagnation.The present invention is on preparation technology, and the part Pericarpium Citri Reticulatae Viride is ground into fine powder, and remainder adopts different extracting method according to the not same-action of various flavour of a drug, and preparation technology's maximum possible extracts the effective ingredient of each flavour of a drug.
Show that through pharmacodynamic study gastric infusion can significantly reduce E continuously 2, P, LH content, significantly reduce the rat height of nipples and reduce the uterus coefficient, significantly reduce plasma viscosity, though whole blood viscosity and packed cell volume are had reduction trend, not statistically significant.Pathological examination shows, the Pu Chai addiction sheet that disappears can obviously reduce acinus and forms, and suppresses acinus epithelial proliferation, acinus expansion and secretions hypertrophy, and cyclomastopathy is had good inhibitory effect; The swollen granulation tissue hyperplasia of rat cotton balls there is obvious inhibitory action; The Pu Chai mice ear that addiction sheet 1,0.5g/k g group xylol causes that disappears has obvious inhibitory action; The Pu Chai mouse writhing reaction that addiction sheet 1g, 0.5g/kg Dichlorodiphenyl Acetate cause that disappears has the obvious suppression effect.
Show through toxicologic study: the Wistar rat oral gavage, in successive administration (filling stomach) 26 weeks, reversibility observed for 2 weeks.General state through observing rat administration treated animal after reaching drug withdrawal during the administration, growth promoter, diet, drinking-water, defecation etc. there is no unusual, the weight of animals and organ index and matched group be no significant difference relatively all, every detection index: routine blood test (erythrocyte, leukocyte, hemoglobin, platelet, leukocyte classification); Thrombinogen; Blood biochemical is learned and is checked relatively all no significant differences of (aspartic acid aminotransferase, ALT, alkali phosphatase, total protein, albumin, blood urea nitrogen, total gallbladder are with alcohol, blood glucose, creatinine, total bilirubin) and matched group, and the main organs specimen mirror of pathological observation and censorship rat is checked down and be there is no the pathological change relevant with drug toxicity.Show that the Pu Chai addiction sheet toxicity that disappears is lower, the clinical dosage oral administration safety is bigger.
Prove determined curative effect of the present invention through zoopery; Long term toxicity test proves that the present invention takes (3~6 months) and has no side effect substantially in therapeutic process.Adopt regulating thoroughfare and controlling vessel ruling by law to treat cyclomastopathy, not only can correct hormone disturbance, also can suppress the generation of breast carcinoma.
The specific embodiment
Embodiment 1
1, Herba Taraxaci, Radix Bupleuri, Pericarpium Citri Reticulatae Viride, Bulbus Fritillariae Thunbergii, Radix Curcumae, Fructus Trichosanthis, Radix Angelicae Sinensis and Rhizoma Cyperi are cleaned dried, get Herba Taraxaci 400 grams, Radix Bupleuri 200 grams, Pericarpium Citri Reticulatae Viride 200 grams, Bulbus Fritillariae Thunbergii 200 grams, Radix Curcumae 200 grams, Fructus Trichosanthis 300 grams, Radix Angelicae Sinensis 200 grams, Rhizoma Cyperi 200 grams, Pericarpium Citri Reticulatae Viride, Rhizoma Cyperi parch, the 110g Pericarpium Citri Reticulatae Viride is pulverized, and it is standby to cross 100 mesh sieves;
2, ethanol extraction: Radix Bupleuri, Bulbus Fritillariae Thunbergii, Radix Angelicae Sinensis are added the alcohol reflux twice that 6-12 doubly measures 40-90%, filter then, the extractum of relative density 1.10-1.40 with filtrate recycling ethanol and when being concentrated into 60 degrees centigrade;
3, water extraction: Pericarpium Citri Reticulatae Viride, Herba Taraxaci, Fructus Trichosanthis, Radix Curcumae and Rhizoma Cyperi that pulverizing is remaining, decoct with water three times, each amount of water is 6-12 times, decocts 1-4 hour at every turn, filters the extractum of relative density 1.10-1.40 when filtrate is concentrated into 60 degrees centigrade then;
4, combination drying:,, pulverize the back and add the appropriate amount of auxiliary materials mixed pelletization in 50-80 degree centigrade of drying under reduced pressure with two kinds of extractum of gained in step 2 and the step 3 and Pericarpium Citri Reticulatae Viride fine powder mixing.
Can make tablet, capsule or electuary as required, when preparation tablet and capsule, the adjuvant of adding is generally starch, dextrin or magnesium stearate.During the preparation electuary, the adjuvant of adding is generally sugar or other sweeting agents.
Embodiment 2
Herba Taraxaci, Radix Bupleuri, Pericarpium Citri Reticulatae Viride, Bulbus Fritillariae Thunbergii, Radix Curcumae, Fructus Trichosanthis, Radix Angelicae Sinensis and Rhizoma Cyperi cleaned dry, get Herba Taraxaci 2000 grams, Radix Bupleuri 1000 grams, Pericarpium Citri Reticulatae Viride 1000 grams, Bulbus Fritillariae Thunbergii 1000 grams, Radix Curcumae 1000 grams, Fructus Trichosanthis 1500 grams, Radix Angelicae Sinensis 1000 grams, Rhizoma Cyperi 1000 grams, Pericarpium Citri Reticulatae Viride, Rhizoma Cyperi parch, Pericarpium Citri Reticulatae Viride 550g is pulverized, and it is standby to cross 100 mesh sieves.Other step is with embodiment 1.
Embodiment 3
Herba Taraxaci, Radix Bupleuri, Pericarpium Citri Reticulatae Viride, Bulbus Fritillariae Thunbergii, Radix Curcumae, Fructus Trichosanthis, Radix Angelicae Sinensis and Rhizoma Cyperi cleaned dry.Get 1200 parts of Herba Taraxacis, 600 parts of Radix Bupleuri, 600 parts on Pericarpium Citri Reticulatae Viride, 600 parts of Bulbus Fritillariae Thunbergiis, 600 parts of Radix Curcumaes, 900 parts of Fructus Trichosanthis, Radix Angelicae Sinensis 600 grams and Rhizoma Cyperi 600 grams, Pericarpium Citri Reticulatae Viride 310g is pulverized in Pericarpium Citri Reticulatae Viride, Rhizoma Cyperi parch, and it is standby to cross 100 mesh sieves; Other step is with embodiment 1.
Effect experiment
The medicine that the present invention makes, through zoopery, the cyclomastopathy rat had significant therapeutical effect, reduce estradiol, progesterone and lutropin content, rat hypertrophy uterus significantly dwindles, pathological examination prompting can obviously minimizing acinus forms, and suppresses acinus epithelial proliferation, acinus expansion and secretions hypertrophy, improves the ductal epithelium hyperplasia degree; The mice internal organs pain that Dichlorodiphenyl Acetate causes has significant inhibitory effect; The rat proliferative inflammation that cotton balls is caused has significant inhibitory effect; The chmice acute inflammation that xylol causes also has significant inhibitory effect; Can reduce under the pathological state plasma viscosity in the hemorheology of rat.
In zoopery, the medicine that the present invention makes is a tablet, the called after Pu bavin addiction sheet that disappears, and the medicine that contrast is tried is a RUKANG PIAN, specifically tests as follows:
Be subjected to reagent thing: the Pu Chai addiction sheet that disappears, be Film coated tablets, show sepia after removing film-coat, every gram contains crude drug 3.42 grams, and Science and Technology Ltd. provides by the Jilin great achievement, lot number: 070504; RUKANG PIAN, safe and sound Taisho Pharmaceutical company limited factory produces, lot number: 20060520.The equal gastric infusion of said medicine, matched group give same volume (20ml/kg) water.
Reagent: estradiol benzoate injection, Shanghai General Pharmaceutical Co., ltd., lot number: 050105; Progesterone injection, Zhejiang Province XianJu Pharmacy stock Co., Ltd, lot number: 070502; Dimethylbenzene, organic synthesis chemical plant, Wuhan, lot number: 891009.
Instrument: YDA-IV type blood viscometer, Beijing Hong Run reach the production of development in science and technology company limited.
Animal: Kunming mouse 20 ± 2g, be female, available from Changchun Biological Products Institute, the quality certification number: SCXK-2002-0001; Wistar rat 150~240g is female, and preclinical medicine institute of Jilin University zoopery center provides, quality certification SCXK-(Ji) 2003-0001.
Each experiment body weight difference mice is no more than 4g, and rat is no more than 30g.
Method and result
One, to the influence of hyperplasia of mammary gland model rat
Get 60 of healthy adult unpregnancy female rats, divide equally six groups at random, be respectively matched group, model group, positive control RUKANG PIAN group (0.28g/kg) and Pu bavin disappear addiction sheet group (0.7,0.35,0.175g/kg).Except that matched group, all the other each groups after 25 days, change intramuscular injection every day Progesterone 0.5mg/kg into, continuous 5 days by 0.5mg/kg intramuscular injection every day estradiol benzoate.Each group is irritated stomach and is subjected to the reagent thing, 4 weeks of successive administration simultaneously.After the last administration 24 hours, with the anesthesia of 20% urethane, abdominal aortic blood 5ml, after the 2ml natural subsidence, centrifugal (3000r/min) 5min gets upper serum and is used to measure hormone-content, the sodium citrate anticoagulant of 3ml blood, be used to measure hemorheology index, rat is put to death in the cervical vertebra dislocation then, with second pair of height of nipples of vernier caliper measurement, draw materials through basad maximum tangent plane of nipple again, conventional fixing, paraffin Bao Li, section, HE dyeing, light microscopic is observed histopathology down and is changed.Separating uterus is rejected fatty tissue on every side again, weighs rapidly.The results are shown in Table 1, table 2, table 3.
The influence of addiction sheet that disappear of table 1. Pu bavin to hyperplasia of mammary gland model rat uterus coefficient and height of nipples
Compare * P<0.05, * * P<0.01.x ± S. with model group
The influence of addiction sheet that disappear of table 2. Pu bavin to hyperplasia of mammary gland model rat hormonal readiness
Compare * P<0.05, * * P<0.01.x ± S. with model group
The influence of addiction sheet that disappear of table 3. Pu bavin to hemorheology of rat
Compare * P<0.05, * * P<0.01 with model group
The result shows that rat muscle injection estradiol benzoate and Progesterone cause rat mammary gland hypertrophy, E 2, normal matched group obviously raises for P, LH content, perusal as seen, the rat nipple is red and swollen or increase, and shows that the rat mammary gland model of hyperplasia sets up; Disappear addiction sheet 0.7,0.35g/kg group and RUKANG PIAN 028g/kg group of Pu Chai all can significantly reduce E 2, P, LH content; Significantly reduce the rat height of nipples and reduce the uterus coefficient; Pu Chai addiction sheet 0.7, the 0.35g/kg group that disappears significantly reduces plasma viscosity, though whole blood viscosity and packed cell volume are had reduction trend, not statistically significant.
Pathological examination shows, the mammary gland of control rats all finds no the cyclomastopathy symptom, the obvious hypertrophy of the lobules of mammary gland of model group rat, conduit cavity and acinus expansion are obviously, acinus increases, and lumen of gland and ductal ectasia also have secretions, and disappear addiction sheet high dose group and RUKANG PIAN group of Pu Chai can obviously reduce acinus and form, suppress acinus epithelial proliferation, acinus expansion and secretions hypertrophy, to cyclomastopathy in, low dose group is more effective.
Two, to the bullate influence of rat granuloma
Get 50 of rats, be divided into five groups at random, be respectively matched group, positive control RUKANG PIAN group (0.28g/kg) and the Pu bavin addiction sheet group (0.7,0.35,0.175g/kg) that disappears.The etherization rat, cut off the 1cm osculum respectively after the sterilization of bilateral axillary fossa, each implantation-50mg sterilization cotton balls (autoclaving of bilateral, each adds ampicillin 10mg/ml, 50 ℃ of oven for drying), each organizes preceding 3 days of rat Rhizoma Atractylodis Macrocephalae to postoperative administration every day in 7 day 1 time, amount to 10 times, 1 hour (postoperative 7 days) sacrificed by decapitation rat is cut former otch after the last administration, and cotton balls and hyperplastic tissue are together taken out, 70 ℃ of dryings, accurately weigh, claim to such an extent that weight deducts cotton balls weight and is the granulation tissue hyperplasia degree, the results are shown in Table 2.
The influence of addiction sheet that disappear of table 4. Pu bavin to the rat chronic granulation tissue hyperplasia
With matched group relatively: * * P<0.01*P<0.05.x ± S.
The result shows, disappear addiction sheet 0.7,0.35,0.175g/kg and RUKANG PIAN 0.28g/kg of Pu Chai has obvious inhibitory action to the swollen granulation tissue hyperplasia of rat cotton balls, and the Pu Chai addiction sheet 0.7g/kg group effect that disappears is the strongest.
Three, xylol causes the influence of mice ear
Get 50 of 20g ± 2g mice, be divided into 5 groups at random, be respectively matched group (distilled water), positive control drug RUKANG PIAN group (0.4g/kg) and the Pu bavin addiction sheet group (1,0.5,0.25g/kg) that disappears.The gastric infusion of dosage shown in pressing, every day 1 time, continuous 8 days, matched group gave the equal-volume distilled water.Smeared dimethylbenzene 0.1ml/ only in the Mus auris dextra in 1 hour after the last administration, be coated with back 4h and put to death mice, along the auricle baseline with directly through being that the sharp card punch of 9mm is laid the ears sheet in same area, weigh respectively, calculate its difference as the swelling degree.The results are shown in Table 5.
The disappear influence of the mice auricle swelling degree that addiction sheet xylol causes of table 5 Pu bavin
Compare * P<0.05 with matched group
The result shows: disappear addiction sheet 1,0.5g/kg of RUKANG PIAN 0.4g/kg group and Pu bavin organized the mice ear that xylol causes and had obvious inhibitory action, relatively has significant difference with matched group.
Four, Dichlorodiphenyl Acetate causes the influence of mouse writhing reaction
Get 50 of mices, divide equally five groups at random, be respectively matched group (distilled water), positive control drug RUKANG PIAN group (0.4g/kg) and the Pu bavin addiction sheet group (1,0.5,0.25g/kg) that disappears.Hungry 15h before the administration group mice gastric infusion every day one-time continuous administration 8 days, last administration, 1h respectively organizes mouse peritoneal and only injects 0.8% glacial acetic acid 0.2ml/ after the last administration, and mouse writhing number of times in back 5 minutes~15 minutes injected in record, the results are shown in Table 6.
The table 6. Pu bavin addiction sheet Dichlorodiphenyl Acetate that disappears causes the influence of mice pain reaction
Compare * P<0.05 with matched group
The result shows, RUKANG PIAN 0.4g/kg and the Pu Chai mouse writhing reaction that addiction sheet 1,0.5g/kg Dichlorodiphenyl Acetate cause that disappears has the obvious suppression effect.

Claims (3)

1, a kind of Chinese medicine for the treatment of acute and chronic mammary hyperplasia, it is characterized in that: form 4~20 parts of Herba Taraxacis, 2~10 parts of Radix Bupleuri, 2~10 parts of parch skins, 2~10 parts of Bulbus Fritillariae Thunbergiis, 2~10 parts of Radix Curcumaes, 3~15 parts of Fructus Trichosanthis, 2~10 parts of Radix Angelicae Sinensis, 2~10 parts of stir-fry Rhizoma Cyperis by following parts by weight of traditional Chinese medicines material.
2, the Chinese medicine of treatment acute and chronic mammary hyperplasia according to claim 1, it is characterized in that: form 12 parts of Herba Taraxacis, 6 parts of Radix Bupleuri, 6 parts of parch skins, 6 parts of Bulbus Fritillariae Thunbergiis, 6 parts of Radix Curcumaes, 9 parts of Fructus Trichosanthis, 6 parts of Radix Angelicae Sinensis, 6 parts of stir-fry Rhizoma Cyperis by following parts by weight of traditional Chinese medicines material.
3, the preparation method of Chinese medicine of the described treatment acute and chronic mammary hyperplasia of a kind of claim 1 is characterized in that may further comprise the steps:
A, get the raw materials ready: get 4~20 parts of Herba Taraxacis, 2~10 parts of Radix Bupleuri, 2~10 parts of parch skins, 2~10 parts of Bulbus Fritillariae Thunbergiis, 2~10 parts of Radix Curcumaes, 3~15 parts of Fructus Trichosanthis, 2~10 parts of Radix Angelicae Sinensis by weight, fry 2~10 parts of Rhizoma Cyperis, after aforementioned various medical material is cleaned and is dried, with Pericarpium Citri Reticulatae Viride, Rhizoma Cyperi parch, pulverize about half of Pericarpium Citri Reticulatae Viride, it is standby to cross 100 mesh sieves;
B, ethanol extraction: Radix Bupleuri, Bulbus Fritillariae Thunbergii, Radix Angelicae Sinensis are added the alcohol reflux twice of 6-10 times of weight, concentration 40-90%, filter then, the extractum of relative density 1.10-1.40 with filtrate recycling ethanol and when being concentrated into 60 degrees centigrade;
C, water extraction: half Pericarpium Citri Reticulatae Viride, Herba Taraxaci, Fructus Trichosanthis, Radix Curcumae, Rhizoma Cyperi that pulverizing is remaining, decoct with water three times, the 6-12 that each amount of water is a raw material weight doubly decocted 1-4 hour at every turn, filter the extractum of relative density 1.10-1.40 when filtrate is concentrated into 60 degrees centigrade then;
D, combination drying: with two kinds of extractum of gained and Pericarpium Citri Reticulatae Viride fine powder mixing among step b and the step c, in 50-80 degree centigrade of drying under reduced pressure, pulverize the back and add the appropriate amount of auxiliary materials mixed pelletization, add proper amount of dry starch and lubricant, mixing gets Chinese medicine of the present invention.
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CN107007772A (en) * 2017-05-15 2017-08-04 铜仁市凯盛水产养殖有限公司 A kind of cold water fish forage Chinese medicine additive and preparation method thereof
CN107569651A (en) * 2017-09-13 2018-01-12 李晓艳 A kind of emplastrum for treating proliferation of mammary gland mastitis

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Publication number Priority date Publication date Assignee Title
CN100490885C (en) * 2006-03-21 2009-05-27 吉林省中医中药研究院 Medicine for treating hyperplasia of mammary glands and its preparing method

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Publication number Priority date Publication date Assignee Title
CN102908603A (en) * 2012-10-30 2013-02-06 王凤 Chinese medicament for treating hyperplasia of mammary glands
CN102908603B (en) * 2012-10-30 2014-06-18 王凤 Chinese medicament for treating hyperplasia of mammary glands
CN107007772A (en) * 2017-05-15 2017-08-04 铜仁市凯盛水产养殖有限公司 A kind of cold water fish forage Chinese medicine additive and preparation method thereof
CN107569651A (en) * 2017-09-13 2018-01-12 李晓艳 A kind of emplastrum for treating proliferation of mammary gland mastitis

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