CN101643271A - High-efficiency biological denitrification method for waste water - Google Patents

High-efficiency biological denitrification method for waste water Download PDF

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CN101643271A
CN101643271A CN200910042269A CN200910042269A CN101643271A CN 101643271 A CN101643271 A CN 101643271A CN 200910042269 A CN200910042269 A CN 200910042269A CN 200910042269 A CN200910042269 A CN 200910042269A CN 101643271 A CN101643271 A CN 101643271A
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water
probiotics
waste water
biological denitrification
sodium
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CN101643271B (en
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林炜铁
唐水水
樊杰
朱雅楠
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South China University of Technology SCUT
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00Technologies for wastewater treatment
    • Y02W10/10Biological treatment of water, waste water, or sewage

Abstract

The invention discloses a high-efficiency biological denitrification method for waste water, which is suitable for the treatment of industrial waste water and domestic sewage containing nitrogen. Themethod comprises the following steps: inoculating bacillus to a seed culture medium by volume percent of 5-10%; culturing at 30-35 DEG C at the shake rotating speed of 180-200 r/min for 1-2 days; activating; inoculating the seed solution to a fermentation medium by the volume percent of 5-10% to carry out the fermentation culture for 1-2 days until the number of bacteria in the culture solution exceeds 10<8>/l so as to obtain the required microecologic preparation; and adding the microecologic preparation to waste water containing nitrogen by the volume percent of 5-10%. The method does not require the domestication and the separation of the nitrobacteria so as to greatly save the time and the cost and reduce the work load. The method still has very high removal rate for ammonian under thecondition of high initial ammonian concentration and can remove nitrogen in waste water in a short time.

Description

A kind of high-efficiency biological denitrification method
Technical field
The present invention relates to a kind of high-efficiency biological denitrification method, be specifically related to use genus bacillus, belong to the environmental microorganism technical field the removing of nitrogenous trade effluent and nitrogenous sanitary sewage.
Background technology
Along with the develop rapidly of industrial or agricultural and livestock and poultry breeding industry, environmental problem becomes the focus that people pay close attention to day by day.Wherein, water pollution problems becomes increasingly conspicuous.The environmental hazard that the water body nitrate pollution causes is serious day by day, and topmost consequence is to cause body eutrophication, causes breaking out of wawter bloom.In addition, it also consumes the dissolved oxygen in the water body, and produces nitrite or ammonium nitrite in conversion process, and the health of people and livestock is produced many-sided harm.Being becoming increasingly acute of water environment pollution and water quality eutrophication problem forces more and more countries and area to formulate strict emission control standards.How effectively to handle these trade effluents, reduce the consumption and the secondary pollution of environmental resources simultaneously, become the problem that people pay close attention to.
At the nitrogen element pollution, abroad begin one's study and develop the biological denitrification process technology of waste water from late 1960s, begin to be widely used in the denitrogenation of nutritive substance in municipal effluent and the part trade effluent eighties.China since the eighties in 20th century also the research work of denitrogenation aspect, and obtained certain progress.
Emerging in recent years a kind of Microbial denitrogenation technology mainly is the culturing micro-organisms flora, and the denitrogenation of using microbe flora reaches the purpose of removing the nitrogen in the waste water.The biological denitrificaion method is simple to operate with it, less investment, cost are low, be difficult for causing environmental pollution, many-sided advantages such as efficiencies of nitrogen removal is good, being acknowledged as more economical, effective, little to environmental influence method, also is the most rising method and the widespread use that has obtained countries in the world.
Chinese invention patent application CN200810139207.1 discloses a kind of biological denitrification method for wastewater of industrial cultivation.The disadvantage of this method is on the one hand and need carries out long enrichment domestication to allotrophic nitrobacteria, and a large amount of mask works is arranged, and therefore increased the input of human and material resources, financial resources; This method needs nitrobacteria is adsorbed fixing again on the other hand, so technology is comparatively complicated, operational administrative inconvenience.
Summary of the invention
The objective of the invention is to overcome the shortcoming of prior art, provide that a kind of cost is low, effluent quality good, and the operational process safety and stability is reliable, the method for the nitrogen in the removal waste water of convenient operation and management.
Purpose of the present invention is achieved through the following technical solutions:
A kind of high-efficiency biological denitrification method comprises the steps and processing condition:
(1) preparation of probiotics: genus bacillus is seeded to seed culture medium with 5%~10% volume percent, in temperature is 30~35 ℃, shaking speed is to cultivate activation 1~1.5d under 180~200r/min condition, get seed liquor again, by volume the inoculum size of per-cent 5%~10% is seeded to fermention medium and amplifies fermentation culture 1~2d, and number of bacteria surpasses 10 in nutrient solution 8Individual/l, make required probiotics; Culture condition is 30~35 ℃ of temperature, dissolved oxygen>3.0mg/l;
Described seed culture medium composition of raw materials is to contain peptone 8~12g in the 1000ml water, sodium-chlor 4~6g, and extractum carnis 4~6g, pH 7.2~7.4,121 ℃ of sterilization 20min;
Described fermention medium composition of raw materials is to contain in the 1000ml water: ammonium sulfate 0.5~1.0g, sodium-chlor 0.5~0.8g, dipotassium hydrogen phosphate 0.4~0.6g, glucose 0.5~1.0g, sal epsom 0.1~0.5g, ferrous sulfate 0.05~0.10g, pH7.2~7.4,115 ℃ sterilization 20min;
(2) throw in probiotics to remove the nitrogen in the waste water: the number of bacteria that step (1) makes surpasses 10 8The probiotics of individual/l, by volume the consumption of per-cent 5%~10% adds to nitrogenous effluent.
For further realizing the object of the invention, the seed culture medium composition of raw materials of described microorganism is preferably: contain peptone 10g in the 1000ml water, sodium-chlor 5g, extractum carnis 5g, 121 ℃ of sterilization 20min.
The fermention medium composition of raw materials of described microorganism is preferably: contain ammonium sulfate 0.8g, sodium-chlor 0.6g, dipotassium hydrogen phosphate 0.5g, glucose 0.8g, sal epsom 0.8g, ferrous sulfate 0.08g in the 1000ml water, 115 ℃ of sterilization 20min.
The preferred consumption of per-cent 6%~8% by volume of described probiotics adds to nitrogenous effluent.
Described shaking speed is preferably 180~190r/min.
The present invention makes full use of the advantage (simple to operate, less investment, cost low, efficient etc.) of Microbial denitrogenation, with the genus bacillus probiotics that makes 5%~10% adding to nitrogenous effluent of per-cent by volume after fermentation, thereby reach the purpose of the nitrogen in the efficient removal waste water.
Have following advantage and beneficial effect with respect to prior art the present invention:
(1) probiotics is added in the existing Waste Water Treatment, nitrogenous effluent is carried out nitrated-denitrification and handle, improve the reaction efficiency of former processing system, cut down the consumption of energy, shorten the reaction times, increase the ability and the efficient of treatment system.
(2) the present invention only needs ready probiotics is added in the existing Waste Water Treatment, and easy handling uses manpower and material resources sparingly.Cost is lower on the other hand, can save processing cost greatly.
Embodiment
The present invention is further illustrated below in conjunction with embodiment, need to prove, these embodiment do not constitute limiting the scope of the invention.
1. the mensuration of ammonia-nitrogen content: nessler reagent colorimetry (OD 420)
(1) reagent and preparation
A. nessler reagent: take by weighing the 5.0g potassiumiodide and be dissolved in the 5.0ml water.Other takes by weighing the 2.5g mercury perchloride and is dissolved in the 10ml hot water.Liquor hydrargyri perchloridi slowly is added in the liquor kalii iodide, constantly stirs till the red precipitate (potassiumiodide) that forms is insoluble.After the cooling, add potassium hydroxide solution (15g potassium hydroxide is dissolved in the 30ml water), be diluted with water to 100ml, add the liquor hydrargyri perchloridi of 0.5ml again, left standstill one day, store in the brown narrow-mouthed bottle supernatant liquor standby.
B. sodium tartrate potassium solution: take by weighing the 50g sodium-potassium tartrate and be dissolved in water, heated and boiled to be to drive ammonia, puts coldly, is diluted to 100ml.
C. standard stock solution: take by weighing 0.01g ammonium sulfate and be dissolved in water, move into the 250ml volumetric flask, adding distil water shakes up to the scale constant volume, NH in the solution 4+Concentration be 40 μ g/ml.
(2) making of typical curve
A. draw 0.05ml, 0.1ml, 0.2ml, 0.4ml, 0.5ml and 0.8ml standard stock solution respectively to the 10ml colorimetric cylinder, adding distil water is to the 10ml scale, be prepared into the ammoniumsulphate soln that ammonia nitrogen concentration is respectively 2 μ g/10ml, 4 μ g/10ml, 8 μ g/10ml, 16 μ g/10ml, 20 μ g/10ml, 32 μ g/10ml, it is blank to prepare a pipe simultaneously.
B. spectrophotometry is surveyed the step of ammonia nitrogen concentration: interpolation 0.2ml Seignette salt in the 7 pipe ammoniumsulphate solns that prepare toward previous step respectively, sway gently, and add the 0.2ml nessler reagent again, leave standstill 10min, test OD with spectrophotometer 420
C. with the ammonium sulfate concentrations X-coordinate, OD 420Numerical value be ordinate zou, make the ammonia nitrogen typical curve.
(3) mensuration of sample
Get the 50ml water sample in the 50ml colorimetric cylinder, determination step is tested with typical curve series solution, and colorimetric is the substitution curve as a result, calculates ammonia-nitrogen content (m).
Ammonia nitrogen (N, mg/l)=m * 1000/V
In the formula: m---check in the ammonia-nitrogen content (mg) of sample hose by working curve;
V---volume of water sample (ml).
2. nitrite nitrogen Determination on content: griess reagent spectrophotometry (OD 540)
(1) reagent and preparation
A. ammonium chloride buffer: the 1L volumetric flask adds 0.5L water, accurately adds 20.0ml hydrochloric acid, and the concussion mixing accurately adds 50ml ammonium hydroxide, is diluted with water to scale.Debug to pH value 9.6~9.7 with dilute hydrochloric acid and ammonium hydroxide in case of necessity.
B. Sulphanilic Acid solution: take by weighing the 10g Sulphanilic Acid, be dissolved in 700ml water and the 300ml glacial acetic acid, put mixing in the brown bottle, room temperature preservation.
C.N-1-naphthyl-ethylenediamine solution: take by weighing 0.1gN-1-naphthyl-quadrol, add the dissolving of 60% acetate and be diluted to 100ml, behind the mixing, to brown bottle, in refrigerator, preserve, stable in the week.
D. Sodium Nitrite standard reserving solution: accurately take by weighing the Sodium Nitrite of 250.0mg dry 24h in silica gel drier, be dissolved in water and move in the 500ml volumetric flask, add the 100ml ammonium chloride buffer, thin up is to scale, and mixing is preserved at 4 ℃ of lucifuge places.Every milliliter of Sodium Nitrite that is equivalent to 500 μ g of this solution.
E. the Sodium Nitrite standard is used liquid: before facing usefulness, draw Sodium Nitrite standard reserving solution 1.00ml, place the 100ml volumetric flask, thin up is to scale, and this solution is equivalent to 5.0 μ g Sodium Nitrites for every milliliter.
F. developer: face with preceding N-1-naphthyl-diethylamine and Sulphanilic Acid solution equal-volume are mixed.
(2) making of typical curve
Accurately absorption 0ml, 0.5ml, 1.0ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml Sodium Nitrite standard use liquid (being equivalent to 0 μ g, 2.5 μ g, 5 μ g, 10 μ g, 15 μ g, 20 μ g, 25 μ g Sodium Nitrites) to place the 25ml color-comparison tube respectively, in standard pipe, add the 4.5ml ammonium chloride buffer respectively, add the 5.0ml developer immediately after adding 2.5ml60% acetate, add water to scale, mixing, in the dark leave standstill 25min, use the 1.0cm cuvette, regulate zero point with No. 0 pipe, measure absorbancy, drawing standard curve in wavelength 550nm place.
(3) mensuration of sample
With water sample furnishing neutrality, draw 10.0ml in the 25ml color-comparison tube, determination step is tested with typical curve series solution, and colorimetric is the substitution curve as a result, calculates nitrite content (m).
Nitrite nitrogen (N, mg/l)=m/V
In the formula: m--checks in the quality (mg) of nitrite the sample from typical curve
V--volume of water sample (ml)
3. nitrate nitrogen Determination on content: ultraviolet spectrophotometry (OD 220XOD 275)
(1) reagent and preparation
A. aluminium hydroxide suspension: dissolving 125g potassium aluminium sulfate is heated to 60 ℃ in the 1000ml deionized water, in constantly stirring, slowly add the 55ml strong aqua, place about 1h after, move in the 1000ml graduated cylinder, with deionized water repetitive scrubbing precipitation, do not contain in the washings till the nitrate nitrogen at last.After the clarification, supernatant liquor is all poured out as far as possible, only stayed thick suspension, add the 100ml deionized water at last, should vibrate evenly before using.
B. sodium hydroxide solution: take by weighing 200g sodium hydroxide and be dissolved in the 1000ml deionized water.
C.1mol/l hydrochloric acid soln: measure the 82ml concentrated hydrochloric acid, join in the beaker, add the less water dilution, solution after the dilution is transferred in 1000 milliliters the volumetric flask, washed with water beaker 1-2 time again, also transfer to washing lotion in the volumetric flask, add water to scale again, shake up and get final product.
D. thionamic acid solution: take by weighing 0.8g thionamic acid amine, be dissolved in the 100ml deionized water.
E. the preparation of nitrate standard inventory solution: take by weighing the saltpetre 0.5006g through 110 ℃ of oven dry 4h, be dissolved in the deionized water, move in the 500mL volumetric flask and be diluted to scale, this strength of solution is 1000mg/l.
F. the saltpetre standard is used the preparation of liquid: accurately draw 25.00mL saltpetre standard reserving solution in the 250ml volumetric flask, with the deionized water dilution and be settled to scale, this strength of solution is 100mg/l.
(2) mensuration of sample
A. the pre-treatment of water sample:, iron content dark or organism as the water sample color, get the 100ml water sample in Erlenmeyer flask, add 2ml aluminium hydroxide suspension, place 5min behind the mixing, double-deck filter paper filtering.
B. the water sample 2.0ml (but the direct sampling of clarification water sample) that gets above-mentioned processing is in the 10ml colorimetric cylinder, add 1mol/l hydrochloric acid soln 0.40ml and add 8g/l thionamic acid amine 0.1ml (when being lower than 0.1mg/l as nitrite nitrogen, can not add amino sulfanilic acid), use the pure water constant volume to 10ml, mixing, cuvette with 1cm, make reference with reagent blank, selection 220nm is a predominant wavelength, and 275nm is the baseline wavelength, carry out colorimetric, measure absorbance.
(3) making of typical curve
Add 0.50,1.00,2.00,3.00,4.00 nitrate nitrogen standard reserving solutions respectively in 5 200ml volumetric flasks, be diluted to scale with fresh deionized water, its concentration is respectively 0.25,0.50,1.00,1.50, the 2.00mg/l nitrate nitrogen.Press water determination same operation step and measure absorbancy.Mass concentration (mg/l) with standard specimen is an X-coordinate, and absorbancy is an ordinate zou, the drawing standard curve.
The calculating of the content of nitrate nitrogen: A The school=A 220-2A 275
In the formula: A 220--the 220nm wavelength records absorbancy
A 275--the 275nm wavelength records absorbancy
After trying to achieve the correction value of absorbancy, from typical curve, check in corresponding nitrate nitrogen amount, be water determination result (mg/l).Water sample is if measure after diluting, and then the result should multiply by extension rate.
Embodiment 1
To the removal effect of certain nitrogenous effluent, this ammonia nitrogen in waste water initial concentration is 50mg/l.
A kind of high-efficiency biological denitrification method comprises the steps and processing condition:
(1) preparation of probiotics: genus bacillus (the sharp life peace that the white biotechnology in Zhongshan city hundred company limited produces) is seeded to seed culture medium with 5% of volume percent, in temperature is 30 ℃, shaking speed is to cultivate 1d under the 200r/min condition, activate, get seed liquor again, by volume 5% of per-cent be seeded to fermention medium and amplify fermentation culture 1.5d, number of bacteria surpasses 10 in fermented liquid 8Individual/l.Culture condition is 30 ℃, and dissolved oxygen is 3.0mg/l, makes required probiotics.Described seed culture medium is to contain peptone 10g in the 1000ml water, sodium-chlor 5g, extractum carnis 5g, pH7.2; Described fermention medium is to contain ammonium sulfate 0.5g, sodium-chlor 0.5g, dipotassium hydrogen phosphate 0.4g, glucose 0.5g, sal epsom 0.1g, ferrous sulfate 0.03g in the 1000ml water, and its pH value is 7.2.
(2) throw in probiotics to remove the ammonia nitrogen in the waste water: the probiotics that step (1) is made is 5% adding to waste water of per-cent by volume.
(3) bacterium liquid was taken out in 12 hours in every interval, and detection method is the same, cultivated the actual removal effect of observing ammonia nitrogen, detected the accumulation volume of nitrate and nitrite simultaneously.
(4) concentration of ammonia nitrogen straight line in initial 12 hours drops to 3mg/l, and clearance is up to 94%, and does not almost have the accumulation of nitrite and ammonia nitrogen in the whole process.
Compared with prior art, the domestication that the method for present embodiment need not to carry out nitrobacteria with separate, saved time and cost greatly, reduced workload simultaneously; Secondly, present embodiment only needs ready probiotics to be added in the existing Waste Water Treatment the simple easy handling of technology; Once more, present embodiment still has high ammonia nitrogen removal frank (present embodiment is 94%, and prior art is 63%~92%) under the condition of high ammonia nitrogen initial concentration.
Embodiment 2
To the removal effect of certain nitrogenous effluent, this ammonia nitrogen in waste water initial concentration is 100mg/l.
A kind of high-efficiency biological denitrification method comprises the steps and processing condition:
(1) preparation of probiotics: genus bacillus (the sharp life peace that the white biotechnology in Zhongshan city hundred company limited produces) is seeded to seed culture medium with 5% of volume percent, in temperature is 32 ℃, shaking speed is to cultivate 1d under the 150r/min condition, activate, get seed liquor again, by volume 5% of per-cent be seeded to fermention medium and amplify fermentation culture 1d, number of bacteria surpasses 10 in fermented liquid 8Individual/l.Culture condition is 32 ℃, and dissolved oxygen is 5.0mg/L, makes required probiotics.Described seed culture medium is to contain peptone 12g in the 1000ml water, sodium-chlor 4g, and extractum carnis 4g, pH 7.4; Described fermention medium is to contain in the 1000ml water: ammonium sulfate 1.0g, sodium-chlor 0.8g, dipotassium hydrogen phosphate 0.6g, glucose 1.0g, sal epsom 0.5g, ferrous sulfate 0.10g, pH7.4.
(2) throw in probiotics to remove the ammonia nitrogen in the waste water: the probiotics that step (1) is made the by volume consumption of per-cent 8% adds to waste water.
(3) bacterium liquid was taken out in 12 hours in every interval, and detection method is the same, cultivated the actual removal effect of observing ammonia nitrogen, detected the accumulation volume of nitrate and nitrite simultaneously.
(4) concentration of ammonia nitrogen straight line in initial 12 hours drops to 10mg/l, and clearance is up to 90%, and does not almost have the accumulation of nitrate and nitrite in the whole process.
Compared with prior art, the domestication that present embodiment need not to carry out nitrobacteria with separate, saved time and cost greatly, reduced workload simultaneously; Secondly, present embodiment only needs ready probiotics to be added in the existing Waste Water Treatment the simple easy handling of technology; Once more, present embodiment still has high ammonia nitrogen removal frank (present embodiment is 90%, and prior art is 63%~92%) under the condition of high ammonia nitrogen initial concentration.
Embodiment 3
To the removal effect of certain nitrogenous effluent, this ammonia nitrogen in waste water initial concentration is 200mg/l.
A kind of high-efficiency biological denitrification method comprises the steps and processing condition:
(1) preparation of probiotics: genus bacillus (the sharp life peace that the white biotechnology in Zhongshan city hundred company limited produces) is seeded to seed culture medium with 10% of volume percent, in temperature is 35 ℃, shaking speed is to cultivate 1d under the 180r/min condition, activate, get seed liquor again, by volume 10% of per-cent be seeded to fermention medium and amplify fermentation culture 1.5d, number of bacteria surpasses 10 in fermented liquid 8Individual/l.Culture condition is 35 ℃, and dissolved oxygen is 5.0mg/L, makes required probiotics.Described seed culture medium is to contain peptone 8g in the 1000ml water, sodium-chlor 6g, and extractum carnis 6g, pH 7.2; Described fermention medium is to contain in the 1000ml water: ammonium sulfate 0.7g, sodium-chlor 0.7g, dipotassium hydrogen phosphate 0.6g, glucose 0.6g, sal epsom 0.2g, ferrous sulfate 0.07g, pH7.2.
(2) throw in probiotics to remove the ammonia nitrogen in the waste water: the probiotics that step (1) is made the by volume consumption of per-cent 10% adds to nitrogen-containing wastewater.
(3) bacterium liquid was taken out in 12 hours in every interval, and detection method is the same, cultivated the actual removal effect of observing ammonia nitrogen, detected the accumulation volume of nitrate and nitrite simultaneously.
(4) concentration of ammonia nitrogen straight line in initial 12 hours drops to 30mg/l, and clearance is up to 85%, and does not almost have the accumulation of nitrate and nitrite in the whole process.
Compared with prior art, the domestication that present embodiment need not to carry out nitrobacteria with separate, saved time and cost greatly, reduced workload simultaneously; Secondly, present embodiment only needs ready probiotics to be added in the existing Waste Water Treatment the simple easy handling of technology; Once more, present embodiment still has high ammonia nitrogen removal frank (present embodiment is 85%, and prior art is 63%~92%) under the condition of high ammonia nitrogen initial concentration.

Claims (5)

1, a kind of high-efficiency biological denitrification method is characterized in that comprising the steps and processing condition:
(1) preparation of probiotics: genus bacillus is seeded to seed culture medium with 5%~10% volume percent, in temperature is 30~35 ℃, shaking speed is to cultivate activation 1~1.5d under 180~200r/min condition, get seed liquor again, by volume the inoculum size of per-cent 5%~10% is seeded to fermention medium and amplifies fermentation culture 1~2d, number of bacteria surpasses 108/1 in nutrient solution, makes required probiotics; Culture condition is 30~35 ℃ of temperature, dissolved oxygen>3.0mg/l;
Described seed culture medium composition of raw materials is to contain peptone 8~12g in the 1000ml water, sodium-chlor 4~6g, extractum carnis 4~6g, pH7.2~7.4,121 ℃ sterilization 20min;
Described fermention medium composition of raw materials is to contain in the 1000ml water: ammonium sulfate 0.5~1.0g, sodium-chlor 0.5~0.8g, dipotassium hydrogen phosphate 0.4~0.6g, glucose 0.5~1.0g, sal epsom 0.1~0.5g, ferrous sulfate 0.05~0.10g, pH7.2~7.4,115 ℃ sterilization 20min;
(2) throw in probiotics to remove the nitrogen in the waste water: the number of bacteria that step (1) makes surpasses 10 8The probiotics of individual/l, by volume the consumption of per-cent 5%~10% adds to nitrogenous effluent.
2, high-efficiency biological denitrification method according to claim 1 is characterized in that: the seed culture medium composition of raw materials of described microorganism is: contain peptone 10g in the 1000ml water, sodium-chlor 5g, extractum carnis 5g, 121 ℃ of sterilization 20min.
3, high-efficiency biological denitrification method according to claim 1, it is characterized in that: the fermention medium composition of raw materials of described microorganism is: contain ammonium sulfate 0.8g, sodium-chlor 0.6g, dipotassium hydrogen phosphate 0.5g, glucose 0.8g, sal epsom 0.8g, ferrous sulfate 0.08g in the 1000ml water, 115 ℃ of sterilization 20min.
4, high-efficiency biological denitrification method according to claim 1 is characterized in that: the described probiotics by volume consumption of per-cent 6%~8% adds to nitrogenous effluent.
5, high-efficiency biological denitrification method according to claim 1 is characterized in that: described shaking speed is 180~190r/min.
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CN102703350A (en) * 2012-05-29 2012-10-03 北京大学 Application of salt-tolerant nitrogen and phosphorus removing bacillus alitudinis to wastewater treatment
CN104003519A (en) * 2014-05-28 2014-08-27 杭州拓瑞博科技有限公司 Method for preparing nitrogen nutritive salt by using nitrogenous wastewater
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CN105400861A (en) * 2014-09-11 2016-03-16 北京大北农科技集团股份有限公司 Method for flux quantitative determination of nitrogen reducing capability of strain
CN104694426A (en) * 2015-02-13 2015-06-10 桂林市环境保护科学研究所 Sieving of high-nitrification-capacity nitrobacteria and application of high-nitrification-capacity nitrobacteria in treatment of organic solid wastes
TWI601687B (en) * 2016-06-23 2017-10-11 亞氨科技股份有限公司 Ammonia decomposition apparatus and method thereof

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