CN101613415B - Method for de-coloring soybean polysaccharide - Google Patents
Method for de-coloring soybean polysaccharide Download PDFInfo
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- CN101613415B CN101613415B CN2009100413573A CN200910041357A CN101613415B CN 101613415 B CN101613415 B CN 101613415B CN 2009100413573 A CN2009100413573 A CN 2009100413573A CN 200910041357 A CN200910041357 A CN 200910041357A CN 101613415 B CN101613415 B CN 101613415B
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Abstract
The invention discloses a method for de-coloring soybean polysaccharide, particularly a method for de-coloring the extract of soybean polysaccharide by ion exchange resin. The method comprises the following concrete steps: (1) preparing soybean extract; and (2) allowing the soybean extract to pass through a diatomite layer, further conducting the ion exchange chromatography on the soybean extract by using ion exchange columns, and drying to obtain the required soybean polysaccharide. By de-coloring the extract of soybean polysaccharide by the ion exchange resin, the invention can remove the pigment and organic impurities in soybean polysaccharide; the obtained soybean polysaccharide has the advantages of pure-white and fine color, low protein content and high purity; in addition, by de-coloring the extract of soybean polysaccharide by ion exchange resin, the invention can further simplify the treatment procedure, specifically, the good de-coloring effect can be achieved by only passing through the ion exchange columns; and the content of impurities in the product is low as the spray-drying process is adopted.
Description
Technical field
The present invention relates to technical field of food additives, be specifically related to soybean polysaccharide, relate in particular to a kind of decoloring method of soybean polysaccharide.
Background technology
Nutrient components of soybean is abundant, and since ancient times, China just begins the product of human consumption soybean or soybean production.Soybean polysaccharide (Soybean Polysaccharide) is to extract and the purified water-soluble polysaccharide from soybean; have and to stop proteinic cohesion and precipitation down at sour environment (PH≤4.0); in protected protein matter, do not increase the function of system viscosity; make product have salubrious mouthfeel; soybean polysaccharide not only can be used as a kind of very fine stablizer and is applied in the products such as acidic drinks, whey beverage, and the function raw material that also can be used as the complementary diets fiber simultaneously uses.
A scale is not also had in the production of soybean polysaccharide at present, and the decolouring and the purifying process that are mainly reflected in product are also more backward.The industrial production soybean polysaccharide mainly uses soda acid settling process and active carbon adsorption to come the soybean polysaccharide liquid glucose is decoloured.The soda acid settling process is that the difference according to the solubleness of pigment in different pH solvents removes, though technology is fairly simple, cost is lower, DeGrain.Decolorizing with activated carbon is to utilize its physical adsorption ability, pigment has conjugated double bond structures, can be by activated carbon adsorption, but the molecule of soybean polysaccharide and the viscosity of solution are all bigger, when using activated carbon to decolour, to the requirement height of activated carbon order number, when using grain active carbon to decolour, decolorizing effect is not obvious, and soybean polysaccharide liquid still has tangible tawny; As use powder activated carbon instead and decolour, then too high because of the soybean polysaccharide fluid viscosity, decolorizing efficiency is low, and the polysaccharide liquid after the decolouring also has a certain amount of pigment and is little yellow.
More than two kinds of technologies all can not fully remove organic impuritys such as free albumen and pigment in the soybean polysaccharide liquid, therefore the albumen impurity that contains in the finished product that obtains is many, causes that yield reduces, quality of item descends, thereby has inevitably caused waste economically.
Ion exchange resin is a kind of porousness cross-linked polymer, has higher specific surface area, thereby has good adsorptive power.Nonionic adsorption resin belongs to two phase structure: polymer phase and hole.Polymer phase is to be piled up by numerous nano grade polymer particulates to form, and is the hole between the particulate.Can regulate particle size by adjusting the synthetic method, and aperture and specific surface area etc., thereby make resin have different absorption properties.In addition, polymeric adsorbent can be divided into four kinds of nonpolar, Semi-polarity, polarity and strong polarity again by surface properties.Different surface propertieies makes polymeric adsorbent possess different adsorption mechanisms and absorption property, therefore makes resin obtain many-sided purposes.There are some researches show, replace gac commonly used to decolour, not only can accelerate filtration velocity with exchange resin, and decolorizing effect, also reach the purpose of impurity elimination simultaneously, thereby improve the quality of product.Resin has ionization simultaneously except that decolouring, can play ion exchange reaction with the ion in the solution, add resins for universal use also have stronger ligand group (OH ,-NH3), can play complexing action to metal ion, thereby remove the solution metal ion, play a part impurity elimination.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, providing a kind of can remove pigment and organic impurity in the soybean polysaccharide liquid, the decoloring method of the soybean polysaccharide that gained soybean polysaccharide color is pure white, exquisiteness and purity are high.
Above-mentioned purpose of the present invention is achieved by following scheme:
A kind of decoloring method of soybean polysaccharide, this method is to adopt ion exchange resin that pigment in the soybean polysaccharide liquid and organic impurity are adsorbed removal, because pigment has conjugated double bond structures, therefore ion exchange resin can come charged pigment molecular is exchanged by adsorption, reaches the decolouring purpose.
The resin that degree of crosslinking is high is stronger to the ionic selectivity, the macroporous structure resin choice is less than gel type resin, because the viscosity of soybean polysaccharide crude extract is big, the molecule of polysaccharide is also big, so ion exchange resin of the present invention is selected anionite-exchange resin, the anionite-exchange resin that can select any one those skilled in the art to use always, can both realize in the inventive method removal, acrylic acid series strongly basic anionite-exchange resin such as A850 that produces as Britain's technology and A860 to impurity such as the pigment adsorptivity of soybean polysaccharide extracting solution and protein, the PCK series plastics, MN200 super large mesh anionite-exchange resin, and weak base anion-exchange resins such as A100 and A105 or the like; Strongly basic anionic resin is more obvious to the adsorption of the pigment molecular in the liquid glucose, therefore preferred strongly basic anion exchange resin; Consider from aspects such as the adsorptivity of pigment and selectivity, ion exchange resin of the present invention is more preferably A850 anionite-exchange resin, A860 anionite-exchange resin and MN200 super large mesh anionite-exchange resin, and best preferred version is a MN200 super large mesh anionite-exchange resin.
Above-mentioned A860, A850, A100, A105 and MN200 plasma exchange resin are the model of ion exchange resin, and sale is all arranged on market.
In the decoloring method of above-mentioned soybean polysaccharide, in order to make the Impurity removal better effects if, can also be before ion exchange resin, earlier the soybean polysaccharide extracting solution is passed through diatomite layer, diatomite has characteristics such as porosity is big, adsorptivity is stronger, therefore can be used as filtering medium, the partial impurities in the soybean polysaccharide extracting solution is got rid of earlier.
The decoloring method of above-mentioned soybean polysaccharide, it specifically comprises the steps:
(1) preparation soybean extract;
(2) with soybean extract by after the diatomite layer, adopt ion exchange column to carry out ion exchange chromatography again, obtain required soybean polysaccharide after the drying.
In the above-mentioned steps (1), the preparation of soybean extract is sophisticated operation in the prior art, can adopt those skilled in the art to carry out the conventional way acquisition soybean extract that soybean is extracted, also can adopt preferred version provided by the invention: with the soybean bean dregs is starting material, the soybean bean dregs after soaking under the alkaline condition, are adopted enzyme to handle and obtain the soybean polysaccharide extracting solution; Described soybean bean dregs can adopt those skilled in the art to carry out the soybean bean dregs of using always when soybean extract prepares, also can adopt the soybean bean dregs after deproteinated and the skimming treatment, its protein content is 16% ~ 22% (proteinic quality accounts for the per-cent of whole soybean bean dregs total mass).
Above-mentioned the soybean bean dregs are soaked under alkaline condition, alkaline condition is meant that the pH scope is 8 ~ 11.
Above-mentioned soybean bean dregs employing enzyme after soaking under the alkaline condition is handled obtains the soybean polysaccharide extracting solution, and described enzyme is handled and is meant that the employing cellulase carries out conventional enzyme processing, as adopting cellulase XM66D or XM90.
In the above-mentioned steps (2), diatomaceous consumption is 0.3% ~ 1% of a soybean polysaccharide extracting liquid volume, and promptly the soybean polysaccharide liquid of every 100ml adopts the diatomite of 0.3 ~ 1g.
In the above-mentioned steps (2), ion exchange resin can adopt any anionite-exchange resin, as acrylic acid series strongly basic anionite-exchange resin such as A850 and A860, PCK series plastics, MN200 super large mesh anionite-exchange resin and weak base anion-exchange resins such as A100 and A105, preferred A850 anionite-exchange resin, A860 anionite-exchange resin and MN200 super large mesh anionite-exchange resin are more preferably MN200 super large mesh anionite-exchange resin.
In the above-mentioned steps (2), the amount of filler of ion exchange resin is 400 ~ 700g, handles the soybean polysaccharide liquid of 3L, and the aspect ratio of ion exchange column is 3: 1 ~ 10: 1, and the ratio of ion exchange resin and soybean polysaccharide extracting solution is about 1: 4.
In the above-mentioned steps (2), the condition of ion exchange chromatography can be carried out appropriate selection according to kind, the character of soybean polysaccharide extracting solution and institute's spent ion exchange resin, can be with reference to following parameter during concrete operations: the temperature of ion exchange chromatography be chosen between 25 ~ 60 ℃, pH is between 7 ~ 8, the concentration of soybean polysaccharide extracting solution is 4 ~ 6%, and the speed that the soybean polysaccharide extracting solution is crossed post is 0.5 ~ 20SV (SV is meant the amount of liquid/ion exchange resin capacity that per hour passes through).
In the above-mentioned steps (2), before carrying out ion exchange resin, can carry out pre-treatment to ion-exchanger earlier, select alkaline pre-treatment, pretreating reagent can be sodium hydroxide; The routine operation of this area is adopted in described pre-treatment.
In the above-mentioned steps (2), drying can adopt those skilled in the art's routine operation, as spraying drying, warm air drying or vacuum lyophilization etc., preferably spray drying.
It is pure white to adopt decoloring method of the present invention can prepare color, and foreign protein content is few, and purity can reach the soybean polysaccharide more than 80%.
Compared with prior art, the present invention has following beneficial effect:
1. the present invention adopts ion exchange resin to the processing of decolouring of soybean polysaccharide extracting solution, and not only the pigment removal effect is good, and impurity such as protein are also had the removal effect;
2. the present invention passes through diatomite with the soybean polysaccharide extracting solution earlier, utilize diatomaceous strong adsorption that the Impurity removal in the extracting solution is fallen a part, and then carry out ion exchange resin absorption, impurity such as pigment and protein are removed, the removal effect of two steps makes that the foreign matter content in the finished product is very low, the purity height;
3. the present invention adopts ion exchange resin to the processing of decolouring of soybean polysaccharide extracting solution, has also simplified treatment scheme, only needs post just can realize good decolorizing effect, and the present invention simultaneously also adopts spraying drying, makes that foreign matter content is low in the product;
4. the present invention uses first cellulase that bean dregs are handled, and carries out heat afterwards and put forward these two steps the soybean bean dregs are extracted under alkaline environment, has improved the extraction yield of polysaccharide.
Embodiment
Below in conjunction with specific embodiment the present invention is done to describe further, but specific embodiment is not done any qualification to the present invention.
The decolouring of embodiment 1 ion exchange resin is handled
The soybean bean dregs of present embodiment are commercially available.
Get MN200 super large mesh anionite-exchange resin (commercially available) 600g, after employing sodium hydroxide carries out pre-treatment earlier, the dress post, the aspect ratio of ion column is 5: 1.
Present embodiment adopts ion exchange resin to the processing of decolouring of soybean polysaccharide extracting solution, and its concrete steps are as follows:
(1) in the soybean bean dregs, add entry and fully stir half an hour after, centrifugal removal supernatant liquor, adding water in centrifugal sediment, to regulate whole system pH be 5.0 (the system pH regulator by 5.0 o'clock, is helped the reaction of cellulase), add cellulase and carry out the enzyme processing, the time is 1h.After the enzyme treatment step finishes whole system pH being transferred to is 8.0, temperature regulation to 100 ~ 100 ℃, (this step is to carry out heat to extract to keep 1.5h to carry out the heat extraction, and can make the cellulase deactivation), then obtain soybean polysaccharide extracting solution (these two kinds of methods combine and can add the extraction yield that improves soybean polysaccharide);
(2) get above-mentioned soybean polysaccharide extracting solution 500ml and pass through diatomite layer, diatomaceous consumption is 3g, carrying out ion chromatography with the MN200 super large mesh anion-exchange resin column for preparing then handles, the column flow rate excessively of soybean polysaccharide extracting solution is 2SV, collect the liquid behind the post, obtained the required soybean polysaccharide of present embodiment after the spraying drying.
Present embodiment prepares the gained soybean polysaccharide, and its color, protein content and purity are as shown in table 1.
The decolouring of embodiment 2 ion exchange resin is handled
The soybean bean dregs of present embodiment are commercially available.
Get and float the A850 ion exchange resin 600g that Lai Te (China) company limited produces, after employing sodium hydroxide carries out pre-treatment earlier, the dress post, the aspect ratio of ion column is 7: 1.
Present embodiment adopts ion exchange resin to the processing of decolouring of soybean polysaccharide extracting solution, and its concrete steps are as follows:
(1) in the soybean bean dregs, add entry and fully stir half an hour after, centrifugal removal supernatant liquor, in centrifugal sediment, adding water, to regulate whole system pH be 5.0, adds cellulase and carry out enzyme and handle, the time is 1h.After the enzyme treatment step finishes whole system pH being transferred to is 8.0, and temperature regulation to 100 ~ 100 ℃ keep 1.5h to carry out heat extraction, then obtain the soybean polysaccharide extracting solution;
(2) get above-mentioned soybean polysaccharide extracting solution 500ml and pass through diatomite layer, diatomaceous consumption is 5g, carrying out ion chromatography with the MN200 super large mesh anion-exchange resin column for preparing then handles, the column flow rate excessively of soybean polysaccharide extracting solution is 3SV, collect the liquid behind the post, obtained the required soybean polysaccharide of present embodiment after the warm air drying.
Present embodiment prepares the gained soybean polysaccharide, and its color, protein content and purity are as shown in table 1.
The decolouring of embodiment 3 gacs is handled
The soybean bean dregs of present embodiment are commercially available.
Present embodiment adopts gac to the processing of decolouring of soybean polysaccharide extracting solution, and its concrete steps are as follows:
(1) in the soybean bean dregs, add entry and fully stir half an hour after, centrifugal removal supernatant liquor, in centrifugal sediment, adding water, to regulate whole system pH be 5.0, adds cellulase and carry out enzyme and handle, the time is 1h.After the enzyme treatment step finishes whole system pH being transferred to is 8.0, and temperature regulation to 100 ~ 100 ℃ keep 1.5h to carry out heat extraction, then obtain the soybean polysaccharide extracting solution;
(2) get above-mentioned soybean polysaccharide extracting solution 500ml and pass through diatomite layer; diatomaceous consumption is 5g; with the activated particles gac it is decoloured then; the consumption of grain active carbon is 10g; the aspect ratio of carbon post is 6: 1 behind the dress post; the flow velocity of soybean polysaccharide extracting solution is 2SV, obtains the required soybean polysaccharide of present embodiment after the spraying drying.
Present embodiment prepares the gained soybean polysaccharide, and its color, protein content and purity are as shown in table 1.
The decolouring of embodiment 4 gacs is handled
The soybean bean dregs of present embodiment are commercially available.
Present embodiment adopts gac to the processing of decolouring of soybean polysaccharide extracting solution, and its concrete steps are as follows:
(1) in the soybean bean dregs, add entry and fully stir half an hour after, centrifugal removal supernatant liquor, in centrifugal sediment, adding water, to regulate whole system pH be 5.0, adds cellulase and carry out enzyme and handle, the time is 1h.After the enzyme treatment step finishes whole system pH being transferred to is 8.0, and temperature regulation to 100 ~ 100 ℃ keep 1.5h to carry out heat extraction, then obtain the soybean polysaccharide extracting solution;
(2) get above-mentioned soybean polysaccharide extracting solution 500ml and pass through diatomite layer; diatomaceous consumption is 2.5g; with the activated particles gac it is decoloured then; the weight of grain active carbon is 10g; the aspect ratio of carbon post is 4: 1; the flow velocity of soybean polysaccharide extracting solution is 2SV/ hour, obtains the required soybean polysaccharide of present embodiment after the spraying drying.
Present embodiment prepares the gained soybean polysaccharide, and its color, protein content and purity are as shown in table 1.
The different embodiment of table 1 prepare result's contrast of gained soybean polysaccharide
As can be seen from Table 1, compare with embodiment 4 with embodiment 3, the decolorizing effect of embodiment 1 and embodiment 2 preparation gained soybean polysaccharide is better, and protein content is low, purity is high, illustrates that thus ion-exchange resin decolorization treatment effect of the present invention obviously is better than methods such as gac commonly used in the prior art.The protein content of embodiment 1 preparation gained soybean polysaccharide is lower than the protein content of embodiment 2 preparation gained soybean polysaccharide in addition, and purity is than the height of embodiment 2, during decolouring therefore of the present invention was handled, the preferably spray drying method was handled crossing post liquid behind the ion exchange resin.
Claims (8)
1. the decoloring method of a soybean polysaccharide is characterized in that this method is to adopt ion exchange resin to the processing of decolouring of soybean polysaccharide extracting solution;
Described ion exchange resin is A850 anionite-exchange resin, A860 anionite-exchange resin or MN200 super large mesh anionite-exchange resin.
2. according to the described decoloring method of claim 1, it is characterized in that described ion exchange resin to the soybean polysaccharide extracting solution decolour handle before, earlier soybean extract is handled by diatomite.
3. according to the described decoloring method of claim 1, it is characterized in that this method specifically comprises the steps:
(1) preparation soybean extract;
(2) with soybean extract by after the diatomite layer, adopt ion exchange column to carry out ion exchange chromatography again, obtain required soybean polysaccharide after the drying.
4. according to the described decoloring method of claim 3, it is characterized in that the preparation process of soybean extract is in the described step (1): the soybean bean dregs after soaking under the alkaline condition, are adopted enzyme to handle and obtain the soybean polysaccharide extracting solution.
5. according to the described decoloring method of claim 3, it is characterized in that in the described step (2), diatomaceous consumption is 0.3%～1% of a soybean polysaccharide extracting liquid volume.
6. according to the described decoloring method of claim 3, it is characterized in that in the described step (2), the temperature of ion exchange chromatography is 25～60 ℃, pH is 7～8, and the concentration of soybean polysaccharide extracting solution is 4～6%, and the speed that the soybean polysaccharide extracting solution is crossed post is 0.5～20SV.
7. according to the described decoloring method of claim 3, it is characterized in that in the described step (2) that drying is spraying drying, warm air drying or vacuum lyophilization.
8. according to the described decoloring method of claim 7, it is characterized in that described drying is a spraying drying.
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| CN102477104A (en) * | 2010-11-25 | 2012-05-30 | 苏州宝泽堂医药科技有限公司 | Method for separating and purifying polysaccharide from Hovenia acerba |
| CN102585026B (en) * | 2012-02-20 | 2014-05-21 | 河北食品添加剂有限公司 | Method for continuously, reversely and ultrasonically extracting high-purity water-soluble soybean polysaccharide |
| CN106755613B (en) * | 2016-12-16 | 2020-05-19 | 广州双桥股份有限公司 | Purification method of starch sugar |
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| CN1377590A (en) * | 2001-03-30 | 2002-11-06 | 吴炳新 | Method for producing soybean protein hydrolyate and use |
| CN101177460A (en) * | 2007-12-05 | 2008-05-14 | 浙江工商大学 | Method for extracting water-soluble soybean polysaccharide from soya bean waste and soybean dregs |
| CN101283746A (en) * | 2008-05-14 | 2008-10-15 | 逄镜萍 | Process for extracting the soyabean oligosaccharides by air-float purification and film integration |
| CN101367845A (en) * | 2008-09-27 | 2009-02-18 | 浙江大学 | Decolorization method for raffinose raffinate |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1377590A (en) * | 2001-03-30 | 2002-11-06 | 吴炳新 | Method for producing soybean protein hydrolyate and use |
| CN101177460A (en) * | 2007-12-05 | 2008-05-14 | 浙江工商大学 | Method for extracting water-soluble soybean polysaccharide from soya bean waste and soybean dregs |
| CN101283746A (en) * | 2008-05-14 | 2008-10-15 | 逄镜萍 | Process for extracting the soyabean oligosaccharides by air-float purification and film integration |
| CN101367845A (en) * | 2008-09-27 | 2009-02-18 | 浙江大学 | Decolorization method for raffinose raffinate |
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Denomination of invention: A decolorization method for soybean polysaccharides Effective date of registration: 20230919 Granted publication date: 20110727 Pledgee: Bank of China Limited Guangzhou Pearl River Branch Pledgor: GUANGZHOU FOBIBER BIOLOGICAL INDUSTRY Co.,Ltd. Registration number: Y2023980057418 |