CN101433621A - Chinese medicinal composition for treating asthma of pig as well as preparation method and use thereof - Google Patents
Chinese medicinal composition for treating asthma of pig as well as preparation method and use thereof Download PDFInfo
- Publication number
- CN101433621A CN101433621A CNA2008102439889A CN200810243988A CN101433621A CN 101433621 A CN101433621 A CN 101433621A CN A2008102439889 A CNA2008102439889 A CN A2008102439889A CN 200810243988 A CN200810243988 A CN 200810243988A CN 101433621 A CN101433621 A CN 101433621A
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- ethanol
- medicine composition
- swine enzootic
- bovis seu
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention discloses a traditional Chinese medicine composition for treating swine enzootic pneumonia, a method for preparing the same and application thereof. The traditional Chinese medicine composition is prepared from the following materials by weight portion: 10 to 30 portions of common alstonia bark or leaf, 5 to 15 portions of origanum vulgare and 8 to 24 portions of mullein flower; the origanum vulgare is soaked and distilled to collect distillate; waste residue and waste liquid after distillation are added with the common alstonia bark or leaf and the mullein flower for cooking for three times; the decoction solutions are mixed and concentrated to be thick, added with ethanol to adjust the alcohol concentration to between 60 and 70 percent, and subjected to deposition, filtration and concentration; the concentrated medicine liquid is added with ethanol to adjust the alcohol concentration to between 75 and 80 percent, and subjected to deposition, filtration and concentration; the medicine liquid is added with distilled water, and subjected to cold storage deposition, filtration and concentration; the distillate of the origanum vulgare and the concentrated solution of the common alstonia bark or leaf and the mullein flower are mixed to obtain the traditional Chinese medicine composition. The curing rate of the traditional Chinese medicine composition on the swine enzootic pneumonia is nearly 95 percent; and when the traditional Chinese medicine composition is used to prepare a veterinary drug for treating the swine enzootic pneumonia, the veterinary drug has the advantages of small side effect, safe and convenient use and quick absorption.
Description
Technical field
The invention belongs to the field of traditional Chinese veterinary of treatment livestock and poultry mycoplasma and bacterial infectious disease, relate to a kind of Chinese medicine composition for the treatment of swine enzootic pneumonia and its production and application.
Background technology
Swine enzootic pneumonia is mycoplasmal pneumonia of swine again, and primary disease extensively distributes all over the world, and the sickness rate of China's swine enzootic pneumonia is also higher.Suffer from the dysplasia of pig long-term production, feed conversion rate is low, and economic loss is big.Already develop for pig and brought serious economy loss.Show that according to interrelated data because the infection of mycoplasma, one kilogram of the every weightening finish of growing and fattening pigs is manyed consumption 0.1～0.2 kilogram of feed than health pig.China consumes about 5,000,000,000 kilograms of grain because of primary disease every year more.This disease of treatment has the oxytetracycline medicine at present, though produce effect, the course of treatment long (5～10 days), necessary muscle or intravenous injection are oral invalid during use, and the intravenous injection troublesome poeration, intramuscular injection is too big to the zest of tissue, occurs swelling easily.Kanamycin also has certain effect, but the course of treatment long (10～15 days), also recurrence easily.Because antibiotic being extensive use of, and use lack of standardization even abuse, the drug resistance of pathogen is more and more serious.The harm of fastbacteria is very serious, and it not only makes the antibiotic curative effect weaken, and drug dose strengthens, and prolong the course of treatment, the relapse rate height, and medical expense increases, and the amount of having also leads to complications, and under some situation even make antibiotic lose curative effect, causes suffering from the poultry mortality rate and raises.Particularly nearly 2 years, superpower drug resistance, multidrug resistance pathogen have appearred again on the veterinary clinic, endanger more seriously, almost arrived the available stage of no medicine.Therefore, use Chinese herb on the prevention and treatment swine enzootic pneumonia, mycoplasma is difficult for producing drug resistance, and drug residue free in the livestock products also is very useful to human beings'health.
Summary of the invention:
The object of the present invention is to provide a kind of Chinese medicine composition for the treatment of swine enzootic pneumonia.
Another object of the present invention provides the preparation method of above-mentioned Chinese medicine composition.
A further object of the invention provides above-mentioned Chinese medicine composition and uses in the herbal medicine thing of preparation treatment swine enzootic pneumonia, colibacillosis.
The objective of the invention is to realize by following technical measures:
A kind of Chinese medicine composition for the treatment of swine enzootic pneumonia, said composition is made by following bulk drugs: Cortex Alstoniae scholaris 10～30 weight portions, Adeps Bovis seu Bubali 5～15 weight portions, Herba Verbasci Thapsi 8～24 weight portions.
Above-mentioned treatment swine enzootic pneumonia Chinese medicine composition, this Chinese medicine composition can prepare through the following steps:
Cortex Alstoniae scholaris 10～30 weight portions, Adeps Bovis seu Bubali 5～15 weight portions, Herba Verbasci Thapsi 8～24 weight portions.
A. the Adeps Bovis seu Bubali adding distil water is soaked, distillate is collected in distillation, and is standby;
B. distillation test residue is added Cortex Alstoniae scholaris, Herba Verbasci Thapsi and add distilled water, soak the back and decoct 3 times, collecting decoction also is concentrated into thick shape, adding ethanol, to transfer to determining alcohol be 60%～70%, abundant stirring and evenly mixing, precipitation, filter, filtrate recycling ethanol reclaims medicinal liquid behind the ethanol and adds ethanol again to transfer to determining alcohol be 75%～80%, stir evenly precipitation, filter filtrate recycling ethanol, reconcentration is to there not being the alcohol flavor, medicinal liquid adding distil water cold preservation precipitation is filtered, and filtrate concentrates;
C. the decocting liquid with Adeps Bovis seu Bubali distillate and Cortex Alstoniae scholaris, Herba Verbasci Thapsi merges, and makes appropriate dosage forms.
The present invention treats the swine enzootic pneumonia Chinese medicine composition, can be made into injection and oral liquid;
The preparation method of above-mentioned treatment swine enzootic pneumonia Chinese medicine composition, this method comprises the following steps:
Get Cortex Alstoniae scholaris 10～30 weight portions, Adeps Bovis seu Bubali 5～15 weight portions, Herba Verbasci Thapsi 8～24 weight portions.
A. the Adeps Bovis seu Bubali adding distil water is soaked, distillate is collected in distillation, and is standby;
B. distillation test residue is added Cortex Alstoniae scholaris, Herba Verbasci Thapsi and add distilled water, soak the back and decoct 3 times, collecting decoction also is concentrated into thick shape, adding ethanol, to transfer to determining alcohol be 60%～70%, abundant stirring and evenly mixing, precipitation, filter, filtrate recycling ethanol reclaims medicinal liquid behind the ethanol and adds ethanol again to transfer to determining alcohol be 75%～80%, stir evenly precipitation, filter filtrate recycling ethanol, reconcentration is to there not being the alcohol flavor, medicinal liquid adding distil water cold preservation precipitation is filtered, and filtrate concentrates;
C. the concentrated solution with Adeps Bovis seu Bubali distillate and Cortex Alstoniae scholaris, Herba Verbasci Thapsi merges, and makes appropriate dosage forms.
In addition, the percentage by weight that also can add the total amount of liquid medicine after step c merges in the medicinal liquid is that 0.3%～0.9% chlorobutanol uses as analgesic;
The preparation method of the Chinese medicine composition of above-mentioned treatment swine enzootic pneumonia specifically can follow these steps to preparation:
Get Cortex Alstoniae scholaris 10～30 weight portions, Adeps Bovis seu Bubali 5～15 weight portions, Herba Verbasci Thapsi 8～24 weight portions.
A. the distilled water immersion that the Adeps Bovis seu Bubali medical material is added 3～4 times of amounts of Adeps Bovis seu Bubali medical material gross weight distilled after 24～30 hours, collected distillate, again with the distillate redistillation, collected and heavily steamed liquid, and is standby;
B. distillation test residue and residual liquid are added Cortex Alstoniae scholaris, Herba Verbasci Thapsi is also added Adeps Bovis seu Bubali, Cortex Alstoniae scholaris, 4～5 times of distilled water of the total medical material weight of Herba Verbasci Thapsi, soaking the back decocts 3 times, kept after boiling 0.5～1 hour at every turn, collecting decoction also is concentrated into thick shape, adding 95% ethanol, to transfer to determining alcohol be 60%～70%, fully stirring and evenly mixing precipitates, and filters, filtrate recycling ethanol, reclaim medicinal liquid behind the ethanol and add 95% ethanol again to transfer to determining alcohol be 75%～80%, stir evenly precipitation, filter, the filtrate recycling ethanol reconcentration is to there not being the alcohol flavor, the cold preservation of medicinal liquid adding distil water, precipitation is filtered, and filtrate concentrates;
C. the decocting liquid with Adeps Bovis seu Bubali distillate and Cortex Alstoniae scholaris, Herba Verbasci Thapsi merges, make liquor strength and be every milliliter and contain 2 gram crude drug amounts, add 0.3%～0.9% chlorobutanol of total medicinal liquid weight at last, transfer pH to 7, make injection or oral liquid, the packing flowing steam sterilization promptly.
The present invention treats the Chinese medicine composition of swine enzootic pneumonia and uses in the herbal medicine of preparation treatment swine enzootic pneumonia or colibacillosis of pigs.
Beneficial effect of the present invention:
The present invention draws the marrow of traditional Chinese veterinary medicine, and Cortex Alstoniae scholaris (Alstonia scholaris (L) .R.Brown) has relieving cough and asthma, reducing swelling and alleviating pain effect.Adeps Bovis seu Bubali (Origanum vulgare L.) has the cold expelling of inducing sweat, and removing dampness is regulated the flow of vital energy, the function of human body immunity improving power, and very strong bactericidal action is arranged.Herba Verbasci Thapsi (Verbascum thapsus L.) has the function of heat clearing away, detoxifcation, hemostasis dissipating blood stasis, and pneumonia, influenza virus are had better curative effect.Three kinds of compatibility of drugss reach the effect for the treatment of both the principal and secondary aspects of a disease.The present invention not only has good therapeutical effect to swine enzootic pneumonia, and swine escherichia coli is also had better curative effect.
The present invention is pure Chinese medicine preparation, and side effect is little, and is safe in utilization, and cure rate reaches about 95%, is difficult for recurrence, and pathogen is difficult to produce drug resistance, has avoided the generation of residue of veterinary drug and social effects of pollution.
Further specify curative effect of the present invention with regard to clinical effectiveness of the present invention below:
One, extracorporeal bacteria inhibitor test of the present invention
1. culture medium preparation
1) Cor Bovis seu Bubali immersion 1000mL, sodium chloride 5g, trypsin 2.5g, agar powder 14g, the heating for dissolving after-filtration, adjust pH 8.0, bottling sterilization back is standby.
2) health pig serum 1000mL.
2. drug sensitive test paper preparation: 12 injection of the present invention of using normal saline (blank) respectively and preparing by embodiment 2 methods of the dry aseptic filter paper sheet of diameter 6mm are respectively soaked into 6, placed refrigerator cold-storage 3 hours, take out and separately divide in aseptic plate, in 50 ℃ of dryings 2 hours, the exsiccant scraps of paper are sub-packed in the sterile test tube of silica gel, jam-pack places refrigerator cold-storage standby.Kanamycin susceptibility sheet is purchased in sky, Hangzhou and microorganism reagent company limited.
3. bacterium liquid is equipped with: on preserving swine enzootic pneumonia mycoplasma culture of strains base inclined-plane mycoplasma is moved the culture fluid, cultivated 72 hours for 37 ℃.The mycoplasma bacterial concentration is transferred to 1.5～3.0 * 10
8Ml is used for drug sensitive test.
4. agar plate preparation: will melt and be cooled to the agar culture medium 20ml about 50 ℃, and add 2ml serum and mix, and pour in the sterile petri dish (90mm), getting concentration respectively is 1.5～3.0 * 10
8The bacterium liquid 0.1mL of mL places agar plate central authorities, makes bacterium liquid evenly coat planar surface with scraping the bacterium rod, places about 10 minutes.
5. paste and put the susceptibility sheet: on the agar plate of inoculating mycoplasma, paste and put 2 of susceptibility sheets of the present invention, 2 of kanamycin susceptibility sheets, 2 on the normal saline contrast scraps of paper.Distance between the susceptibility sheet is no less than 24mm, and every kind of susceptibility sheet carries out 3 repetitions, can obtain 6 measured values.
6. hatch: 3 mycoplasma agar culture dishs subsides being put the susceptibility sheet are put 5%CO
2In the gaseous environment, hatched 5 days for 37 ℃, take out, measure antibacterial circle diameter (mm) respectively, antibacterial circle diameter 18.62mm consequently of the present invention, kanamycin antibacterial circle diameter 13.03mm, two groups of comparing differences remarkable (P＜0.05).
Two, clinical experimental study of the present invention
1. medicine: this test is a test drug with the injection of the present invention for preparing by embodiment 2 methods, is the contrast medicine with the kanamycin.
2. detection kit: the swine enzootic pneumonia indirect hemagglutination detection kit is provided by China Veterinery Drug Inspection Office.
3. test method and grouping: select body weight, age similar on certain pig farm, have the state of an illness of swine enzootic pneumonia feature, every pig ear vein blood sampling, separation of serum, measure antibody titer, with the sample serum antibody titer〉1:10, i.e. the above coagulation that " ++ " occur in second hole, be judged to be 40 of male sick pigs, be divided into 3 groups at random, first group of 15 pig used injection for treating of the present invention, every kg body weight 0.3ml, an intramuscular injection, twice of every day.Second group of 15 pig treated every kg body weight 10mg, an intramuscular injection, twice of every day with the kanamycin medicine.Every group of medication natural law decided according to sick pig recovery situation.The 3rd group of 10 not medications of pig compare.
4. efficacy determination: recover normal with spirit, appetite, the frequency of respiration per minute does not have cough and is produce effects about 20 times.Similar substantially before spirit, appetite, frequency of respiration, status of cough and the treatment is invalid.Therapeutic outcome sees Table 1.
Table 1 Chinese medicine is to the swine enzootic pneumonia therapeutic outcome
Three, the present invention is to the clinical observation on the therapeutic effect of swine enzootic pneumonia
Swine enzootic pneumonia is a boar chronic respiratory system diseases that is caused by mycoplasma hyopneumoniae, and the pig of all ages and classes, sex and kind all can infect, and is characteristics with contact, hyperinfection, chronic, high incidence, mainly shows as cough, dyspnea.Its harm is to cause that pig growth and development is obstructed and feed conversion rate declines to a great extent, and adds the sickness rate that it is high, has a strong impact on the economic benefit of raising pigs, and can promote and bring out the generation of other disease simultaneously, and increase the weight of the state of an illness.With Taizhou City, Xiuqian City, the Nantong City of the injection for preparing by embodiment 1 method in Jiangsu Province, treat 1134 of disease pigs altogether, cure 1083, cure rate is 95.5%, the present invention sees Table 2 to treatment pig asthma curative effect statistics.
Table 2 treatment by Chinese herbs swine enzootic pneumonia statistical table
| Unit | Example number (head) | Cure number (head) | Cure rate (%) |
| Pig farm, Xinghua, Jiangsu | 158 | 151 | 95.6 |
| Pig farm, Taizhou City Jiangyan City | 246 | 240 | 97.5 |
| Pig farm, Qidong, Nantong City | 425 | 405 | 95.3 |
| Pig farm, Siyang, Xiuqian City | 305 | 287 | 94 |
| Add up to | 1134 | 1083 | 95.5 |
The present invention treats swine enzootic pneumonia, obtains better curative effect, and 2 days symptoms of general treatment obviously alleviate, and treats recovery from illness in 3～4 days, does not generally recur after the healing, shows that the present invention has very high curative effect.
Four, the present invention is to the clinical observation on the therapeutic effect of piglet pujos blancos disease
The piglet pujos blancos disease is caused by escherichia coli, betides 10～30 age in days piglets.The generation of piglet pujos blancos disease is except that the escherichia coli that infect certain serotype, and also the change with some risk factors such as weather cataclysm, dark and damp cold, colony house filth and feedstuff and feeding manner all has substantial connection, and it is popular that primary disease is endemicity.Because colibacillary serotype is many, so vaccine prevention is difficult to gather effect, is to use antibiotic therapy after the morbidity substantially.Because long-term heavy dose of antibiotic that uses makes colibacillary drug resistance more and more stronger.Though some antibiotic is effective in cure, the course of treatment is long, effect is not remarkable, has a strong impact on the economic benefit of raising pigs.In Xiuqian City, Yangzhou, Yancheng City, the Xuzhou City in Jiangsu the piglet pujos blancos disease is carried out therapeutic test with prepare injection by embodiment 2 methods, treat 924 of disease pigs altogether, cure 892, cure rate is 96.5%.The present invention sees Table 3 to the curative effect statistics of treatment piglet pujos blancos disease.
The sick statistical table of table 3 treatment by Chinese herbs piglet pujos blancos
| Unit | Example number (head) | Cure number (head) | Cure rate (%) |
| Pig farm, Henan, place, Xiuqian City | 208 | 204 | 98 |
| The Yangzhou pig farm | 323 | 214 | 97.2 |
| Pig farm, Yancheng City Xiangshui County | 159 | 151 | 95 |
| Pig farm, Feng County, Xuzhou City | 234 | 223 | 95.2 |
| Add up to | 924 | 892 | 96.5 |
The present invention is a kind of pure Chinese medicinal preparation, and flavour of a drug are few, effective, using dosage is few, safe ready.The present invention can also open up new approach for the treatment swine enzootic pneumonia up to about 95% to the cure rate of porcine mycoplasmal and bacterial infection, has also avoided the generation of residue of veterinary drug and social effects of pollution simultaneously.
The specific embodiment:
The invention will be further described (every weight portion 1000 grams) below in conjunction with specific embodiment:
Embodiment 1:
Prescription: Cortex Alstoniae scholaris 10 weight portions, Adeps Bovis seu Bubali 5 weight portions, Herba Verbasci Thapsi 8 weight portions
Concrete preparation process is as follows:
A. the distilled water immersion 24 hours that Adeps Bovis seu Bubali is added 3 times of Adeps Bovis seu Bubali medical material weight makes the medical material back distillation of fully expanding, and collects the distillate that obtains 2 times of Adeps Bovis seu Bubali medical material weight, with distillate redistillation 1 time, collects the distillate of Adeps Bovis seu Bubali medical material weight 1/2 again, and the bottling sterilization is standby;
B. distillation test residue and residual liquid are added Cortex Alstoniae scholaris, Herba Verbasci Thapsi and add the distilled water of 4 times of Adeps Bovis seu Bubalis, Cortex Alstoniae scholaris, the total medical material weight of Herba Verbasci Thapsi, soaking the back decocts 3 times, kept after boiling 0.5 hour at every turn, and merged three times decocting liquid, filter, and be concentrated into thick shape (relative density 1.2～1.25), adding 95% ethanol, to transfer to the solution determining alcohol be 60%, and fully stirring and evenly mixing precipitates 48 hours, filter filtrate recycling ethanol.Reclaim medicinal liquid behind the ethanol and add 95% ethanol again to transfer to determining alcohol be 75%, stirred evenly precipitation 48 hours, filter, filtrate recycling ethanol, reconcentration is not to there being the alcohol flavor, and medicinal liquid adds 4 times of distilled water cold preservations precipitations of medicinal liquid weight 48 hours, filters, and filtrate concentrates;
C. the concentrated solution with Adeps Bovis seu Bubali distillate and Cortex Alstoniae scholaris, Herba Verbasci Thapsi merges, making liquor strength reaches every milliliter and contains crude drug 2 gram, after adding 0.3% chlorobutanol of total medicinal liquid weight, with percentage by weight is that 10% sodium hydroxide solution is transferred medicinal liquid pH value 7, make injection, flowing steam sterilization promptly.
Embodiment 2:
Prescription: Cortex Alstoniae scholaris 20 weight portions, Adeps Bovis seu Bubali 10 weight portions, Herba Verbasci Thapsi 16 weight portions
Concrete preparation process is as follows:
A. Adeps Bovis seu Bubali is added 4 times of distilled water immersions of Adeps Bovis seu Bubali medical material weight 30 hours, make fully expand back distillation of medical material, collect 2 times the distillate that obtains Adeps Bovis seu Bubali medical material weight, again with distillate redistillation 1 time, collection obtains the distillate of Adeps Bovis seu Bubali medical material weight 1/2, and the bottling sterilization is standby.
B. distillation test residue and residual liquid are added Cortex Alstoniae scholaris, Herba Verbasci Thapsi and add Adeps Bovis seu Bubali, Cortex Alstoniae scholaris, 5 times of distilled water of the total medical material weight of Herba Verbasci Thapsi, the person of frying in shallow oil is 3 times after the immersion, kept after boiling 1 hour at every turn, collecting decoction also is concentrated into thick shape (relative density 1.2～1.25), adding 95% ethanol, to transfer to the solution determining alcohol be 65%, and fully stirring and evenly mixing precipitates 60 hours, filter filtrate recycling ethanol.Reclaim medicinal liquid behind the ethanol and add 95% ethanol again to transfer to determining alcohol be 80%, stirred evenly precipitation 48 hours, filter, filtrate recycling ethanol, reconcentration is not to there being the alcohol flavor, and medicinal liquid adds 2 times of distilled water cold preservations precipitations of medicinal liquid weight 24 hours, filters, and filtrate concentrates.
C. the concentrated solution with Adeps Bovis seu Bubali distillate and Cortex Alstoniae scholaris, Herba Verbasci Thapsi merges, reach every milliliter of concentration that contains crude drug 2 grams, 0.6% the chlorobutanol that adds the weight of total amount of liquid medicine, with percentage by weight is that 10% sodium hydroxide solution is transferred medicinal liquid pH value to 7, the fine straining packing, make injection, flowing steam sterilization promptly.
Embodiment 3:
Prescription: Cortex Alstoniae scholaris 30 weight portions, Adeps Bovis seu Bubali 15 weight portions, Herba Verbasci Thapsi 24 weight portions
Concrete preparation process is as follows:
A. Adeps Bovis seu Bubali is added 3 times of distilled water immersions of Adeps Bovis seu Bubali medical material weight 27 hours, make fully expand back distillation of medical material, collect the distillate that obtains 2 times of Adeps Bovis seu Bubali medical material weight, with distillate redistillation 1 time, collect the distillate that obtains medical material weight 1/2 again, the bottling sterilization is standby;
B. distillation test residue and residual liquid are added Cortex Alstoniae scholaris, Herba Verbasci Thapsi and add Adeps Bovis seu Bubali, Cortex Alstoniae scholaris, 5 times of distilled water of the total medical material weight of Herba Verbasci Thapsi, soaking the back decocts 3 times, kept after boiling 1 hour at every turn, collecting decoction also is concentrated into thick shape (relative density 1.2～1.25), adding 95% ethanol, to transfer to the solution determining alcohol be 70%, and fully stirring and evenly mixing precipitates 48 hours, filter filtrate recycling ethanol.Reclaim medicinal liquid behind the ethanol and add 95% ethanol again to transfer to determining alcohol be 80%, stirred evenly precipitation 48 hours, filter, filtrate recycling ethanol, reconcentration is not to there being the alcohol flavor, and medicinal liquid adds 3 times of distilled water cold preservations precipitations of medicinal liquid weight 48 hours, filters, and filtrate concentrates;
C. the concentrated solution with Adeps Bovis seu Bubali distillate and Cortex Alstoniae scholaris, Herba Verbasci Thapsi merges, reaching every milliliter of concentration that contains crude drug 2 gram, add 0.9% chlorobutanol of the weight of total amount of liquid medicine, is 10% sodium hydroxide solution accent medicinal liquid pH to 7 with percentage by weight, fine straining is made oral liquid.
Claims (6)
1. Chinese medicine composition for the treatment of swine enzootic pneumonia is characterized in that said composition made by following bulk drugs: Cortex Alstoniae scholaris 10～30 weight portions, Adeps Bovis seu Bubali 5～15 weight portions, Herba Verbasci Thapsi 8～24 weight portions.
2. the Chinese medicine composition of treatment swine enzootic pneumonia according to claim 1, the dosage form that it is characterized in that described compositions are injection or oral liquid.
3. the preparation method of the Chinese medicine composition of the described treatment swine enzootic pneumonia of claim 1 is characterized in that this method may further comprise the steps:
A. the Adeps Bovis seu Bubali adding distil water is soaked, distillate is collected in distillation;
B. distillation test residue is added Cortex Alstoniae scholaris, Herba Verbasci Thapsi and add distilled water and decoct 3 times, collecting decoction also is concentrated into thick shape, and adding ethanol, to transfer to the solution determining alcohol be 60%～70%, fully stirring and evenly mixing precipitates, and filters, filtrate recycling ethanol reclaims medicinal liquid behind the ethanol and adds ethanol again to transfer to determining alcohol be 75%～80%, stirs evenly precipitation, filter, filtrate recycling ethanol, reconcentration is to not having alcohol flavor, the cold preservation of medicinal liquid adding distil water, precipitation, filter, filtrate concentrates;
C. the concentrated solution with Adeps Bovis seu Bubali distillate and Cortex Alstoniae scholaris, Herba Verbasci Thapsi merges.
4. the preparation method of the Chinese medicine composition of treatment swine enzootic pneumonia according to claim 3 is characterized in that also adding 0.3%～0.9% chlorobutanol of the total medicinal liquid weight after step c merges.
5. the preparation method of the Chinese medicine composition of treatment swine enzootic pneumonia according to claim 3 is characterized in that this method specifically may further comprise the steps:
A. the distilled water immersion that the Adeps Bovis seu Bubali medical material is added 3～4 times of amounts of Adeps Bovis seu Bubali medical material gross weight distilled after 24～30 hours, collected distillate, again with the distillate redistillation, collected and heavily steamed liquid, and is standby;
B. distillation test residue is added Cortex Alstoniae scholaris, Herba Verbasci Thapsi and add 4～5 times of distilled water of total drug weight, soaking the back decocts 3 times, kept after boiling 0.5～1 hour at every turn, collecting decoction also is concentrated into thick shape, adding 95% ethanol, to transfer to the solution determining alcohol be 60%～70%, fully stirring and evenly mixing precipitates, and filters, filtrate recycling ethanol, reclaim medicinal liquid behind the ethanol and add 95% ethanol again to transfer to determining alcohol be 75%～80%, stir evenly precipitation, filter, the filtrate recycling ethanol reconcentration is to there not being the alcohol flavor, the cold preservation of medicinal liquid adding distil water, precipitation are filtered, and filtrate concentrates;
C. the decocting liquid with Adeps Bovis seu Bubali distillate and Cortex Alstoniae scholaris, Herba Verbasci Thapsi merges, make liquor strength and be every milliliter and contain 2 gram crude drug amounts, add 0.3%～0.9% chlorobutanol of total medicinal liquid weight at last, transfer pH to 7, make injection or oral liquid, the packing flowing steam sterilization promptly.
6. the application of the Chinese medicine composition of claim 1 or 2 described treatment swine enzootic pneumonias in the herbal medicine thing of preparation treatment swine enzootic pneumonia, colibacillosis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102439889A CN101433621B (en) | 2008-12-19 | 2008-12-19 | Chinese medicinal composition for treating asthma of pig as well as preparation method and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102439889A CN101433621B (en) | 2008-12-19 | 2008-12-19 | Chinese medicinal composition for treating asthma of pig as well as preparation method and use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101433621A true CN101433621A (en) | 2009-05-20 |
| CN101433621B CN101433621B (en) | 2011-11-09 |
Family
ID=40708361
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008102439889A Expired - Fee Related CN101433621B (en) | 2008-12-19 | 2008-12-19 | Chinese medicinal composition for treating asthma of pig as well as preparation method and use thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101433621B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103768239A (en) * | 2012-10-24 | 2014-05-07 | 天津中敖生物科技有限公司 | Traditional Chinese medicine composition for preventing and treating swine mycoplasmal pneumonia and preparation method of traditional Chinese medicine composition |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1125106A (en) * | 1994-12-21 | 1996-06-26 | 湖南省常德中药厂 | Chang'an capsule and its preparation method and application |
| CN101084894B (en) * | 2006-06-07 | 2011-11-23 | 中国科学院昆明植物研究所 | Medicine for treating diseases concerned with respiratory and use thereof |
| CN101084951B (en) * | 2006-06-07 | 2011-03-16 | 中国科学院昆明植物研究所 | Medicine for treating diseases concerned with respiratory and preparation method and application thereof |
-
2008
- 2008-12-19 CN CN2008102439889A patent/CN101433621B/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103768239A (en) * | 2012-10-24 | 2014-05-07 | 天津中敖生物科技有限公司 | Traditional Chinese medicine composition for preventing and treating swine mycoplasmal pneumonia and preparation method of traditional Chinese medicine composition |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101433621B (en) | 2011-11-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101214311B (en) | Compound scutellaria injection for treating fowls and domestic animals infectious disease and preparation thereof | |
| CN102755399A (en) | Traditional Chinese medicine composition for resisting coccidiosis | |
| CN104351563A (en) | Functional feed used for preventing pig malignant edema and preparation method of functional feed | |
| CN104208294B (en) | It is a kind of to be used to treat Chinese medicine composition of piglet yellow-white dysentery and preparation method thereof | |
| CN103583898A (en) | Chinese medicinal herb-containing feed additive capable of preventing weaned pig multisystem failure syndrome and pig feed containing same | |
| CN102671011A (en) | Traditional Chinese medicine compound powder for preventing and treating yellow and white scour of piglets and preparation method of traditional Chinese medicine compound powder | |
| CN107184702A (en) | A kind of Chinese medicine composition for preventing and treating poultry proventriculitis ventriculitis and preparation method thereof | |
| CN101433621B (en) | Chinese medicinal composition for treating asthma of pig as well as preparation method and use thereof | |
| CN102973730B (en) | Chinese medicament for animals with functions of clearing heat and removing toxicity, resisting virus and improving body immunity and preparation method thereof | |
| CN103417653B (en) | Veterinary-use compound pure Chinese herbal medicine for treating coccidium and preparation method of oral liquid of same | |
| CN103751244B (en) | A kind of Chinese medicine oral liquid for the treatment of infectious coryza of chicken and preparation method thereof | |
| CN103656320B (en) | For preventing and treating the oral administered compound Chinese medicine preparation of birds anemofrigid cold, influenza | |
| CN102846893B (en) | Chinese medicine composition for expelling parasite in animal and preparation method thereof | |
| CN102302575B (en) | Oral medicament for treating white scour of piglets and preparation method thereof | |
| CN101829257B (en) | Traditional Chinese medicine oral liquid for treating chicken colibacillosis and preparation method thereof | |
| CN101780199B (en) | Traditional Chinese medicine breast perfusate for treating mastitis of dairy cattle and preparation method thereof | |
| CN108553570A (en) | A kind of preparation method and applications for the biological bacterium solution stimulated the secretion of milk for sow | |
| CN103720930B (en) | A kind of Chinese medicine composition treating bovine mastitis | |
| CN115607614B (en) | Traditional Chinese medicine composition for treating colibacillosis of livestock and poultry and preparation method thereof | |
| CN108543010A (en) | A kind of preparation method and applications for treating pig asthma syndrome bacterium solution | |
| CN103690771B (en) | Be used for the treatment of medicine of lamb colibacillosis and preparation method thereof | |
| CN103893424B (en) | A kind of traditional Chinese medicine oral liquid for preventing and treating high-quality chicken cyanomycosis and preparation method thereof | |
| CN113730481B (en) | Traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, suppository and preparation method | |
| CN107582650A (en) | A kind of Chinese medicine composition for being used to prevent and treat sow mastitis | |
| CN101953879B (en) | Microbial ecological agent for quickly and efficiently preventing and treating pig high fever |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111109 Termination date: 20121219 |