CN101407833A - Preparation of edible fungus beta-dextran - Google Patents
Preparation of edible fungus beta-dextran Download PDFInfo
- Publication number
- CN101407833A CN101407833A CNA2008101223181A CN200810122318A CN101407833A CN 101407833 A CN101407833 A CN 101407833A CN A2008101223181 A CNA2008101223181 A CN A2008101223181A CN 200810122318 A CN200810122318 A CN 200810122318A CN 101407833 A CN101407833 A CN 101407833A
- Authority
- CN
- China
- Prior art keywords
- sporophore
- liquid
- quality
- dried powder
- beta
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention provides a preparation method of edible fungi Beta-glucan. In the method, ultrasonic pretreatment, neutral cellulose, hemi-cellulase, the cellulase enzyme digested by a neutral protease mixed enzyme, hemicellulose (like xylan, gelose and a plurality of heteroglycans) and proteins (and peptides) are mainly adopted; a high-temperature amylase is added for the enzyme digestion of the amyloid simultaneously when hot water is extracted; and a target is obtained through a sequent ethanol deposition and the edible fungi Beta-glucan with better activity is further obtained through the hyperfiltration of a film with an interception of 10,000. The preparation method has the advantages that: the product purity of the edible fungi Beta-glucan by adopting the method is high (55.2 to 82.3 percent); the preparation process does not have the residues of poisonous and harmful chemical reagents; the product is safe; the technical condition is moderate and is easy to be operated; and the preparation method is suitable for preparing all the edible fungi Beta-glucan and is suitable for commercial production.
Description
(1) technical field
The present invention relates to a kind of preparation method of edible fungus beta-dextran, especially the preparation method of water-soluble beta-glucan in the edible mushrooms.
(2) background technology
Active polysaccharide β-(1 → 3)-D-dextran extensively is present in occurring in nature, is the cell walls composing type composition of bacterium, yeast, fungi, edible mushrooms, cereal and marine alga.At present, both at home and abroad the investigator has isolated the polysaccharide that the hundreds of kind has antitumor, enhancing immunity, anti-ageing and anti-oxidant activity from edible mushrooms, in the world, edible fungi polysaccharide is called as " biological response effector " (BiologicalResponse Modifier).Conclude through summing up, there is consistent relatively relation in the edible mushrooms functional polysaccharide on conclusive primary structure, the active polysaccharide that extracts from thalline generally is made up of glucose, and the β on the glucose main chain-1, β on 3-glycosidic bond and the side chain-1,6 glycosidic bond is antitumor necessary (Methacanon et al.2005; Mantovani et al.2008), Bohn et al.1995, below this structure with β-1,3/1, the 6-dextran is represented.The saccharan of β-1 → 2, β-1 → 4 and α-configuration does not then have biological activity or activity very weak mostly, has shown good active aspect the β-1,3/1 simultaneously, 6-dextran other outside antitumor yet.In view of edible fungus beta-dextran, β-1,3/1 especially, the good efficacy of 6-dextran is more and more admitted on the edible fungi polysaccharide world market with its content or purity at present as quality evalution and price standard,
In recent years, beta-glucan preparation both domestic and external or the patent of extracting and paper mainly also concentrated in these several raw materials of highland barley, oat, barley and yeast in cereals source; And for the beta-glucan that edible mushrooms is originated, the separation and purification after just the polysaccharide that comprises some edible mushroomss such as glossy ganoderma, Grifola frondosa, mushroom, Hericium erinaceus (Bull. Ex Fr.) Pers. being extracted is difficult to industrialization.
Aspect beta-glucan preparation or extracting method and operational path, mainly also concentrate on autolysis method, acid system, alkaline process, acid-base method, enzyme process at present and (use wherein several single enzymes, as amylase, saccharifying enzyme, proteolytic enzyme, cellulase and polygalacturonase) etc., add lixiviate behind the water, after regulating parameters such as pH, temperature, solid-liquid ratio, extraction time, get corresponding dextran behind the ethanol sedimentation.
Because raw material sources are different, applied purposes differs, and is not quite similar in method as the preparation of the edible fungus beta-dextran of medicines and health protection food intermediate and above-mentioned cereals beta-glucan.As acid, alkaline process, the mechanical means during not only to production has damage, and also the edible safety to product brings potential hazard, cause the degraded of original polysaccharide chain when acid-base method extracts in addition easily, broken ring structure, and the dextran extracted of acid-base method is because poorly water-soluble, oral validity is not good yet.And traditional direct heat flooding, the resulting Crude polysaccharides of edible fungi polysaccharide extracting method of ethanol sedimentation, wherein also has more protein, and some fibre element, starch based and non-β-1,3/1, the saccharide residue of 6-or connecting key, polysaccharide content generally accounts for 30～50% of crude extract in the general edible mushrooms Crude polysaccharides, and the active beta-glucan purity that wherein contains only accounts for 15～40%.Also there is bibliographical information to adopt organic solvent such as Sevag method to remove wherein contained protein in the Crude polysaccharides, but has the residual and production plant organic solvent safety anti-explosive problem of the potential murder by poisoning of organic solvent equally.
Press the small-sized edible fungi polysaccharide manufacturer of 20 tons of scales of the previous annual output of order, if full scale production Crude polysaccharides, annual value of production can only reach 1,000 ten thousand yuan, if can realize the prepared in high purity of edible fungus beta-dextran, (the 2500 yuan/kg of reserve price of 2500-10000 unit/kg) calculates the product of high beta-glucan content (purity 〉=50) by the existing market valency, just can make the output value reach 5,000 ten thousand yuan, remarkable in economical benefits.
Still do not have at present the corresponding edible fungus beta-dextran preparation or the paper and the patent report of extracting method, more all is that edible fungi polysaccharide extracts.And relevant cereals or yeast beta-dextran preparation and extraction mainly concentrate on following exemplary process:
But one, the preparation method of the beta-glucan of existing relative reference
1, the preparation of cereals beta-glucan
1. the preparation of beta-glucan in the oat bran: oat bran → pulverize → 50 orders that sieve → degreasing (Virahol and the sherwood oil) → enzyme that goes out → add water stirring extraction (enzyme-added destarching) → centrifugation (residue second extraction) → collection supernatant liquor → Deproteinization (hydrochloric acid accent isoelectric precipitation) → alcohol to analyse → centrifugation → drying → dextran crude product → ammonium sulfate purifying → centrifugation → beta-glucan
In operational path, use Virahol and sherwood oil organic solvent and corrosive chemical HCl, increase the Product Safety risk.
2. the preparation of beta-glucan in the naked oats: naked oats → cleaning screens → pulverizes → sieve → drying → oat bran → degrease → enzyme → desolventizing that goes out → pulverize → sieve (60 order) → enzyme process destarching → alkaline extraction → centrifugal (4000r/min) → supernatant liquor (crude extract) → enzymolysis destarching → enzymolysis deproteinize → alcohol to analyse → centrifugal → precipitation → drying → beta-glucan crude product
In operational path, use alkaline extraction, the extraction equipment during not only to production causes infection, and has increased product edible safety risk, in addition, for edible mushrooms, except starch and protein, also to consider to remove inactive cellulose family composition.
3. the preparation of beta-glucan in the barley: extract the centrifugal 15min of 1h → 6000g → extract residue and centrifugal once more under Fructus Hordei Vulgaris bran → adding 1mol/L NaOH (1: 50) → room temperature, merge supernatant liquor → regulate supernatant liquor to pH 6.5 → add CaCl with HCl with 1: 50 1mol/L NaOH
2To final concentration be 70mg/L, add high temperature resistant α-Dian Fenmei 1mL/L, under 96 ℃ of conditions, stir to keep 1h → be cooled to room temperature, regulate the centrifugal 15min of pH 4.5 → 6000g with HCl; Waste → add ethanol to final concentration is 50%, spend the night under 4 ℃ of conditions → the centrifugal 15min of 6000g → water-soluble the rough glue that suspends, with 50% washing with alcohol 2 times, centrifugal, mixing throw out → activated carbon decolorizing 2 times gently in water, dialysis tubing dialysis → lyophilize powdering beta-glucan
Use NaOH and HCl, have above-mentioned similar safety problem.
2, the preparation of beta-glucan in the yeast
1. acid system extracts
Cereuisiae fermentum slag → drying → adding 0.5mol/L acetic acid solution is 50 ℃ of following reaction 3h → centrifugal treating → throw outs washing 2 times → then with absolute ethanol washing decolouring → anhydrous diethyl ether washing dehydration → spraying drying → yeast beta-dextran
Obtaining is sour insoluble glucan, and foreign matter content such as protein, mannosans is many in the product, need be further purified, complex process is big to the equipment corrosion loss.
2. alkaline process extracts
Cereuisiae fermentum → drying → adding 1mol/L sodium hydroxide solution is in 90 ℃ of following hydrolysis reaction 3h → hydrolyzed solution filtration → throw out washing 2 times → then with absolute ethanol washing decolouring, anhydrous diethyl ether washing dehydration → spraying drying → yeast beta-dextran
It is alkali-insoluble glucan that this method obtains, and polysaccharide yield is low but foreign matter content is few, pollutes greatlyyer, and it is higher to consume energy, big to the equipment corrosion loss.
3. alkali-enzyme process
Yeast powder is through washing, decolouring back → add NaOH solution → hydrolysis 2h under 80 ℃ of water bath condition of 3% → centrifugal according to the ratio of 150mL: 10g, washing → adjustment pH to 8.5～9.0, add 600U/g Sumizyme MP (in dried yeast powder) again, in 55 ℃ of following hydrolysis 24h → centrifugal → precipitation, drying → β-(1,3)-D-dextran finished product
Complex process is used alkali lye, and is not only big to the equipment corrosion loss, and increased the potential security risks of product.
4. self-dissolving-alkali-enzyme process
Cereuisiae fermentum → 50 ℃ self-dissolving 6h → interpolation 100U/g yeast papoid that wets, continue centrifugal → precipitation behind the self-dissolving 18h with 2% NaOH solution disperse → centrifugal behind 80 ℃ of water bath processing 3h → precipitation with 3～4 times → vacuum lyophilization of distilled water cleaning → pulverize yeast beta-dextran
Use alkali lye equally, have above-mentioned similar problem.
3, refining behind the edible mushrooms Crude polysaccharides deproteinated
The preparation technology of batch production edible mushrooms Crude polysaccharides is as follows usually: fruit body of edible fungi → 60-100 ℃ of dry 1-3h → pulverizing → 60～100 order sieve → add water (for 20-40 times of weight of raw material) → hot water extraction 2-4h → be cooled to room temperature → Plate Filtration or centrifugal → clear liquid → concentrate → add final concentration is that ethanol → throw out of 70%～90% adds suitable quantity of water and disperses (3-5 of weight of precipitate doubly) → spraying drying → edible mushrooms Crude polysaccharides finished product
Refining behind the conventional edible mushrooms Crude polysaccharides deproteinated: edible mushrooms Crude polysaccharides finished product → add 5-10 water redissolution → organic solvent deproteinated → evaporation doubly to desolvate → make with extra care
Wherein organic solvent deproteinated method comprises: Sevag method, Sevag reagent (it is 4: 1 mixed solution that chloroform-propyl carbinol is mixed with volume ratio) and polysaccharide ratio 1: 3 fully after the vibration, are removed lower floor's protein; Trichloroacetic acid method.
This method is just removed protein wherein, there is not specific aim to remove to wherein Mierocrystalline cellulose, starchy material, crucial is to have used chloroform, trichoroacetic acid(TCA) etc. certain toxic organic solvent is arranged, cost height not only, though can remove most of solvent through evaporation, but unavoidable small amount of residual is considered the security when product is edible, is not suitable for applicability production.
4, edible fungus beta-dextran separation and purification preparation
Edible mushrooms Crude polysaccharides finished product → add 5-10 water doubly redissolve → to use ion-exchange, the beta-glucan component of certain molecular weight is identified → obtained to post separation means → separation and purification → structures such as gel chromatography
This method is given birth to factory and is used investment greatly owing to will use column chromatography method, the operational requirement height, and production efficiency is low, identifies that it is expensive to waste time and energy but also need carry out product structure.
(3) summary of the invention
The present invention is according to the composition characteristic and the application security of edible fungi raw materials, and purpose is to realize that a kind of security is good, and versatility and production practical application are strong, and the edible fungus beta-dextran preparation method of purity height (〉=50%).
The technical solution used in the present invention is:
A kind of preparation method of edible fungus beta-dextran, described method comprises: get and be crushed to 60～100 purpose fruit body of edible fungi dried powders, adding quality is the water of 5～10 times of sporophore powder quality, the feed liquid that obtains is in 45KHz, unit power 50～80W/kg feed liquid, room temperature supersound process 10～30 minutes, the feed liquid after the supersound process add the water that quality is 10～20 times of sporophore dried powder quality again, and add CaCl
2To its concentration be 100～300ppm, the neutral cellulase that adds 400～800IU/g sporophore dried powder, the neutral protease of the hemicellulase of 600～1000IU/g sporophore dried powder and 1000～2000IU/g sporophore dried powder, 40～60 ℃ of insulated and stirred are after 0.5～1.5 hour, the alpha-amylase that adds 200～500IU/g sporophore dried powder, 85～95 ℃ of insulated and stirred 1～2 hour, be cooled to room temperature, centrifugal, get supernatant liquor, concentrating under reduced pressure, it is 60～80% to carry out alcohol precipitation that concentrated solution adds ethanol to ethanol volumetric concentration, getting precipitation and add quality and redissolve for the water of 10～15 times of precipitation quality, is 10000 membrane ultrafiltration with the molecular retention amount, and trapped fluid is evaporated to 1/5～1/3 of original volume, drying obtains described edible fungus beta-dextran.
Usually be the aqueous ethanolic solution that adds 90～98% (volumetric concentrations) at alcohol precipitation, the amount of adding is 2～4 times (by volumes) of concentrated solution.
Owing to still do not have edible fungus beta-dextran preparation targetedly or extracting method at present, and all there is the problem of production application or security aspect in above-mentioned referential method.Consider the edible mushrooms substance characteristics, the present invention is by integrating supersound process, the impurity elimination of plurality of enzymes enzymolysis, the hot water lixiviate, redissolve behind the target compound ethanol sedimentation, the ultrafiltration impurity elimination, technological methods such as spraying drying or lyophilize have been set up the edible fungus beta-dextran preparation were established of a kind of safe, practical and highly versatile and high purity (〉=50%).
The present invention mainly adopts ultrasonic pre-treatment, neutral cellulase, hemicellulase, neutral protease mixed enzyme enzymolysis cellulase, hemicellulose are (as xylan, Polygalactan, and some heteroglycans) and protein (and peptide class), add alpha-amylase enzymolysis starchy material simultaneously in hot water extraction, obtain target compound by follow-up ethanol sedimentation,, further obtain active better edible fungus beta-dextran through the membrane ultrafiltration of 10,000 interceptions.
Wherein: the 1. ultrasonic pre-treatment of 10～30min, it is loose not only to help material to organize, and is beneficial to follow-up enzymolysis and fully contacts effectively impurity elimination behind the substrate, and can shorten the last hot water extraction time.
2. consider to shorten enzymolysis time, adopt the mixed enzyme enzymolysis, considered 3 kinds of enzymes temperature of reasonable enzymolysis each other, and the consistence of pH value and material system nature pH value, and after the impurity elimination of mixed enzyme enzymolysis, as overlay on the degraded of the hemicelluloses such as xylan on material surface, also be beneficial to the subsequent extracted of contained target beta-glucan.
3. shortening extraction time of consideration simultaneously and enzymolysis are removed starchy material, add alpha-amylase when hot water extraction.
4. when alcohol precipitation, because Mierocrystalline cellulose, hemicellulose, protein are degraded into relative small-molecule substance with starch based impurity substantially by above-mentioned enzymolysis, make the enrichment of the macromolecular beta-glucan precipitation of target compound by controlling rational whole alcohol concn (60%～80%).
5. the general molecular weight of the functional beta-glucan of edible mushrooms of considering bibliographical information has adopted 10,000 interception membrane ultrafiltration to be further purified active beta-glucan more than 10,000 in technology final section.
Used neutral cellulase is liquid neutral cellulase, enzyme 10000～30000IU/mL alive.
Used hemicellulase (Hemicellulase) is liquid hemicellulase, enzyme 20000～40000IU/mL alive.
Used neutral protease is liquid neutral protease (neutral proteinase), enzyme 20000～40000IU/mL alive.
Used alpha-amylase is liquid alpha-amylase, enzyme 10000～30000IU/mL alive.
Described fruit body of edible fungi is one of following: mushroom fruiting body, Grifola Frondosa sporophore, Ganoderma sporophore, Agaricus blazei Murrill sporophore, Phellinus sporophore.
Concrete, described method is as follows:
(1) get fruit body of edible fungi, 60～100 ℃ of dryings are crushed to 60～100 orders, get the fruit body of edible fungi dried powder, adding quality is the water of 8～10 times of sporophore powder quality, and the feed liquid that obtains is in 45KHz, unit power 50～80W/kg feed liquid, room temperature supersound process 20～30 minutes;
(2) feed liquid after the supersound process adds the water that quality is 10～20 times of sporophore dried powder quality again, and adds CaCl
2To its concentration be 100～300ppm, add the liquid neutral cellulase of 20000IU/mL of 400～800 IU/g sporophore dried powders, the liquid hemicellulase of 30000IU/mL of 600～1000IU/g sporophore dried powder and the liquid neutral protease of 30000IU/mL of 1000～2000IU/g sporophore dried powder, 40～60 ℃ of insulated and stirred enzymolysis 0.5～1.5 hour;
(3) enzymolysis solution adds the liquid alpha-amylase of 20000IU/mL of 200～500IU/g sporophore dried powder, 90～95 ℃ of insulated and stirred 1～2 hour, be cooled to room temperature, centrifugal, get supernatant liquor, concentrating under reduced pressure, it is 60～80% to carry out alcohol precipitation that concentrated solution adds ethanol to ethanol volumetric concentration, get precipitation and add the water redissolution of quality for 10～15 times of precipitation quality, it with the molecular retention amount 10000 membrane ultrafiltration, trapped fluid is evaporated to 1/5～1/3 of original volume, and spraying drying obtains described edible fungus beta-dextran.
Adopt method of the present invention, several edible mushroomss such as mushroom, Grifola frondosa, glossy ganoderma, Agaricus blazei Murrill, Phellinus have been carried out the beta-glucan preparation, products obtained therefrom is through the narrow spectrum fluorescent method detection of beta-glucan, result such as following table.
The purity of 5 kinds of edible fungus beta-dextran products of gained is between 55.2%～82.3%, all be higher than 50% target purity, extraction yield is between 0.87%～4.77%, do not comprise follow-up alcohol precipitation, ultrafiltration, spraying drying, the extraction part 2～4h consuming time of front, with the edible mushrooms Crude polysaccharides extraction time basically identical of routine, but in the conventional edible mushrooms Crude polysaccharides beta-glucan purity only between 15%-40%.And adopt preparation method of the present invention, no poisonous and harmful chemical agent residue, product safety; Technical qualification gentlenesses such as ultrasonic, enzymolysis that adopts and ultrafiltration, easy handling; Present method is according to the total constitutive property of edible mushrooms, and highly versatile is applicable to all edible fungus beta-dextran preparations.
In sum, beneficial effect of the present invention is mainly reflected in: adopt the inventive method, edible fungus beta-dextran product purity height (55.2%～82.3%), preparation process does not have the poisonous and harmful chemical agent residue, product safety, technical qualification gentleness, easy handling, be fit to all edible fungus beta-dextran preparations, be applicable to suitability for industrialized production.
(4) description of drawings
Fig. 1 is the inventive method process flow sheet.
(5) embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:
Embodiment 1:
Technical process is referring to Fig. 1, and mushroom fruiting body → 80 ℃ warm air drying 2h → be crushed to 80 orders → get mushroom fruiting body dried powder 1kg add water → 45KHz of 10kg, power 550W, and room temperature supersound process 20min → add the water of 20kg again, and add the CaCl of 6.2g
2The liquid neutral cellulase 40mL of → adding (enzyme 20000IU/mL alive, Ningxia jade of the He family Bioisystech Co., Ltd), liquid hemicellulase 33.3mL (enzyme 30000IU/mL alive, Ningxia jade of the He family Bioisystech Co., Ltd) and liquid neutral protease 66.7mL (the enzyme 30000IU/mL that lives, Ningxia jade of the He family Bioisystech Co., Ltd), nature pH (is uncomfortable pH, generally 6.5～7.0), 45 ℃ of insulations, mix the liquid alpha-amylase 25mL of enzymolysis 1.5h → adding (enzyme 20000IU/mL alive, Ningxia jade of the He family Bioisystech Co., Ltd), 95 ℃ of hot water stirs and extracts 2h → be cooled to room temperature → 5000r/min, 10min is centrifugal to remove slag → supernatant liquor (about 30L) → be evaporated to edible ethanol → precipitation of 6L → adding 24L, the water that adds 15 times of weight redissolves 1/3 → spraying drying → mushroom beta-glucan finished product that the polysulfone membrane ultrafiltration → trapped fluid of → 1 ten thousand molecular weight cut-off is evaporated to original volume and (detects through fluorescent method, purity 65.6%, extraction yield 4.63%).
Embodiment 2:
Technical process is referring to Fig. 1, Grifola Frondosa sporophore → 80 ℃ warm air drying 2h → be crushed to 100 orders → get 1kg Grifola Frondosa sporophore dried powder add water → 45KHz of 10kg, power 660W, room temperature supersound process 20min → add the water of 15kg again, and add the CaCl of 5.2g
2The liquid neutral cellulase 30mL of → adding (enzyme 20000IU/mL alive), liquid hemicellulase 30mL (enzyme 30000IU/mL alive) and liquid neutral protease 60mL (enzyme 30000IU/mL alive), nature pH (is uncomfortable pH, generally 6.5～7.0), 45 ℃ of insulations, mix the liquid alpha-amylase 15mL of enzymolysis 1.5h → adding (enzyme 20000IU/mL alive), 95 ℃ of hot water stirs and extracts 1.5h → be cooled to room temperature → 5000r/min, 10min is centrifugal to remove slag → supernatant liquor (about 25L) → be evaporated to edible ethanol → precipitation of 6L → adding 14L, the water that adds 10 times of weight of water redissolves 1/3 → spraying drying → grifola frondosa beta-glucose finished product that the polysulfone membrane ultrafiltration → trapped fluid of → 1 ten thousand molecular weight cut-off is evaporated to original volume and (detects through fluorescent method, beta-glucan purity 68.5%, extraction yield 3.60%).
Embodiment 3:
Technical process is referring to Fig. 1, and Ganoderma sporophore → 80 ℃ warm air drying 2h → be crushed to 100 orders → get Ganoderma sporophore dried powder 1kg add water → 45KHz of 6kg, power 560W, and room temperature supersound process 30min → add the water of 13kg again, and add the CaCl of 6g
2The liquid neutral cellulase 40mL of → adding (enzyme 20000IU/mL alive), liquid hemicellulase 33.3mL (enzyme 30000IU/mL alive) and liquid neutral protease 40mL (enzyme 30000IU/mL alive), nature pH (is uncomfortable pH, generally 6.5～7.0), 50 ℃ of insulations, mix the liquid alpha-amylase 10mL of enzymolysis 1.0h → adding (enzyme 20000IU/mL alive), 95 ℃ of hot water stirs and extracts 1.5h → be cooled to room temperature → 5000r/min, 10min is centrifugal to remove slag → supernatant liquor (about 18L) → be evaporated to edible ethanol → precipitation of 6L → adding 9L, the water that adds 15 times of weight redissolves 1/5 → spraying drying → glossy ganoderma beta-glucan finished product that the polysulfone membrane ultrafiltration → trapped fluid of → 1 ten thousand molecular weight cut-off is evaporated to original volume and (detects through fluorescent method, beta-glucan purity 82.3%, extraction yield 0.87%).
Embodiment 4:
Technical process is referring to Fig. 1, and Agaricus blazei Murrill sporophore → 80 ℃ warm air drying 3h → be crushed to 80 orders → get Agaricus blazei Murrill sporophore dried powder 1kg add water → 45KHz of 12kg, power 650W, and room temperature supersound process 20min → add the water of 17kg again, and add the CaCl of 9g
2The liquid neutral cellulase 60mL of → adding (enzyme 20000IU/mL alive), liquid hemicellulase 26.7mL (enzyme 30000IU/mL alive) and liquid neutral protease 66.7mL (enzyme 30000IU/mL alive), nature pH (is uncomfortable pH, generally 6.5～7.0), 50 ℃ of insulations, mix the liquid alpha-amylase of enzymolysis 1h → add, enzyme 20000IU/mL alive, addition 500IU/g (fruit body of edible fungi weight), 95 ℃ of hot water stirs and extracts 2h → be cooled to room temperature → 5000r/min, 10min is centrifugal to remove slag → supernatant liquor (about 25L) → be evaporated to edible ethanol → precipitation of 5L → adding 20L, the water that adds 12 times of weight redissolves 1/3 → spraying drying → Agaricus blazei Murrill beta-glucan finished product that the polysulfone membrane ultrafiltration → trapped fluid of → 1 ten thousand molecular weight cut-off is evaporated to original volume and (detects through fluorescent method, beta-glucan purity 55.2%, extraction yield 3.81%).
Embodiment 5:
Technical process is referring to Fig. 1, and Phellinus sporophore → 100 ℃ warm air drying → be crushed to 100 orders → get Phellinus sporophore dried powder 1kg add water → 45KHz of 5kg, power 480W, and room temperature supersound process 30min → add the water of 10kg again, and add the CaCl of 3.2g
2The liquid neutral cellulase 40mL of → adding (enzyme 20000IU/mL alive); liquid hemicellulase 26.7mL (enzyme 30000IU/mL alive) and liquid neutral protease 43.4mL (enzyme 30000IU/mL alive); nature pH (is uncomfortable pH; generally 6.5～7.0); 45 ℃ of insulations; mix the liquid alpha-amylase 20mL of enzymolysis 1.5h → adding (enzyme 20000IU/mL alive); 95 ℃ of hot water stirs and extracts 2h → be cooled to room temperature → 5000r/min; 5min is centrifugal to remove slag → supernatant liquor (about 12L) → be evaporated to edible ethanol → precipitation of 3L → adding 7L; the water that adds 15 times of weight redissolves 1/4 times → spraying drying → Phellinus beta-glucan finished product that the polysulfone membrane ultrafiltration → trapped fluid of → 1 ten thousand molecular weight cut-off is evaporated to original volume and (detects through fluorescent method; beta-glucan purity 78.7%, extraction yield 2.14%).
Claims (10)
1. the preparation method of an edible fungus beta-dextran, described method comprises: get and be crushed to 60～100 purpose fruit body of edible fungi dried powders, adding quality is the water of 5～10 times of sporophore powder quality, the feed liquid that obtains is in 45KHz, unit power 50～80W/kg feed liquid, room temperature supersound process 10～30 minutes, the feed liquid after the supersound process add the water that quality is 10～20 times of sporophore dried powder quality again, and add CaCl
2To its concentration be 100～300ppm, the neutral cellulase that adds 400～800IU/g sporophore dried powder, the neutral protease of the hemicellulase of 600～1000IU/g sporophore dried powder and 1000～2000IU/g sporophore dried powder, 40～60 ℃ of insulated and stirred are after 0.5～1.5 hour, the alpha-amylase that adds 200～500IU/g sporophore dried powder, 85～95 ℃ of insulated and stirred 1～2 hour, be cooled to room temperature, centrifugal, get supernatant liquor, concentrating under reduced pressure, it is 60～80% to carry out alcohol precipitation that concentrated solution adds ethanol to ethanol volumetric concentration, getting precipitation and add quality and redissolve for the water of 10～15 times of precipitation quality, is 10000 membrane ultrafiltration with the molecular retention amount, and trapped fluid is evaporated to 1/5～1/3 of original volume, drying obtains described edible fungus beta-dextran.
2. the method for claim 1 is characterized in that used neutral cellulase is liquid neutral cellulase, enzyme 10000～30000IU/mL alive.
3. the method for claim 1 is characterized in that used hemicellulase is liquid hemicellulase, enzyme 20000～40000IU/mL alive.
4. the method for claim 1 is characterized in that used neutral protease is liquid neutral protease, enzyme 20000～40000IU/mL alive.
5. the method for claim 1 is characterized in that used alpha-amylase is liquid alpha-amylase, enzyme 10000～30000IU/mL alive.
6. the method for claim 1 is characterized in that described fruit body of edible fungi is one of following:
Mushroom fruiting body, Grifola Frondosa sporophore, Ganoderma sporophore, Agaricus blazei Murrill sporophore, Phellinus sporophore.
7. the method for claim 1, the ethanol that it is characterized in that described adding is 90～98% aqueous ethanolic solutions.
8. the method for claim 1 is characterized in that described 90～98% aqueous ethanolic solutions adding volume is 2～4 times of concentrated solution volume.
9. the method for claim 1 is characterized in that described drying is a spraying drying.
10. the method for claim 1 is characterized in that described method is as follows:
(1) get fruit body of edible fungi, 60～100 ℃ of dryings are crushed to 60～100 orders, get the fruit body of edible fungi dried powder, adding quality is the water of 8～10 times of sporophore powder quality, and the feed liquid that obtains is in 45KHz, unit power 50～80W/kg feed liquid, room temperature supersound process 20～30 minutes;
(2) feed liquid after the supersound process adds the water that quality is 10～20 times of sporophore dried powder quality again, and adds CaCl
2To its concentration be 100～300ppm, add the liquid neutral cellulase of 20000IU/mL of 400～800IU/g sporophore dried powder, the liquid hemicellulase of 30000IU/mL of 600～1000IU/g sporophore dried powder and the liquid neutral protease of 30000IU/mL of 1000～2000IU/g sporophore dried powder, 40～60 ℃ of insulated and stirred enzymolysis 0.5～1.5 hour;
(3) enzymolysis solution adds the liquid alpha-amylase of 20000IU/mL of 200～500IU/g sporophore dried powder, 90～95 ℃ of insulated and stirred 1～2 hour, be cooled to room temperature, centrifugal, get supernatant liquor, concentrating under reduced pressure, it is 60～80% to carry out alcohol precipitation that concentrated solution adds 95% ethanol to ethanol volumetric concentration, get precipitation and add the water redissolution of quality for 10～15 times of precipitation quality, it with the molecular retention amount 10000 membrane ultrafiltration, trapped fluid is evaporated to 1/5～1/3 of original volume, and spraying drying obtains described edible fungus beta-dextran.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101223181A CN101407833A (en) | 2008-11-10 | 2008-11-10 | Preparation of edible fungus beta-dextran |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101223181A CN101407833A (en) | 2008-11-10 | 2008-11-10 | Preparation of edible fungus beta-dextran |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101407833A true CN101407833A (en) | 2009-04-15 |
Family
ID=40571044
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008101223181A Pending CN101407833A (en) | 2008-11-10 | 2008-11-10 | Preparation of edible fungus beta-dextran |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101407833A (en) |
Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869264A (en) * | 2010-06-02 | 2010-10-27 | 文承官 | Mushroom concentrated liquid and drink containing beta-glucan and preparation method thereof |
| CN102106875A (en) * | 2010-12-06 | 2011-06-29 | 浙江工业大学 | Method for extracting water-soluble alpha-glucosidase inhibitor |
| CN102453105A (en) * | 2010-11-02 | 2012-05-16 | 天津济命生生物科技有限公司 | Extraction method for polysaccharide |
| CN102603917A (en) * | 2012-03-22 | 2012-07-25 | 无锡德冠生物科技有限公司 | Method for extracting yeast glucosan by utilizing enzymatic method |
| CN103059160A (en) * | 2011-10-20 | 2013-04-24 | 中国科学院上海药物研究所 | Beta-glucan GFPBW1, its preparation method and application |
| CN103070010A (en) * | 2012-12-27 | 2013-05-01 | 陕西恒田化工有限公司 | Coriolus versicolor strain and method for extracting Coriolus versicolor glucan by utilizing fermentation products of Coriolus versicolor strain |
| CN103304680A (en) * | 2012-03-09 | 2013-09-18 | 中国科学院上海药物研究所 | Beta-glucan, and extraction method and application thereof |
| CN104277135A (en) * | 2014-09-23 | 2015-01-14 | 华南农业大学 | Pleurotus tuber-regium polysaccharide and extraction method thereof |
| CN105628457A (en) * | 2014-10-27 | 2016-06-01 | 内蒙古蒙牛乳业（集团）股份有限公司 | Exaction method and content determination method of yeast beta-glucan in liquid-state milk |
| CN105732250A (en) * | 2016-02-06 | 2016-07-06 | 福建农林大学 | Preparing method for high-purity grifola frondosa polyphenol component |
| CN106036846A (en) * | 2016-06-11 | 2016-10-26 | 张俊辉 | Preparation process of agaricus blazei murill and roselle effervescent tablets |
| CN106491662A (en) * | 2016-11-03 | 2017-03-15 | 淳安千岛湖桑都食用菌专业合作社 | A kind of method for cultivating extraction polysaccharide and flavones simultaneously in waste material from Phellinus |
| CN107467128A (en) * | 2017-07-25 | 2017-12-15 | 合肥台香食品有限公司 | A kind of bread of hypoglycemia patient |
| CN110156910A (en) * | 2019-05-10 | 2019-08-23 | 浙江工业大学 | Yeast dextran extract, composition and the application in preparation prevent constipation preparation |
| CN110305920A (en) * | 2019-07-02 | 2019-10-08 | 泉后(广州)生物科技研究院有限公司 | A kind of active fermentation object and its preparation method and application |
| CN113968919A (en) * | 2021-11-25 | 2022-01-25 | 浙江百山祖生物科技有限公司 | Edible fungus extract without auxiliary materials and preparation method thereof |
| CN114292888A (en) * | 2021-12-30 | 2022-04-08 | 赣州禾绿康健生物技术有限公司 | Preparation process of hericium erinaceus extract capable of improving immunity |
| CN114304353A (en) * | 2021-12-30 | 2022-04-12 | 艾苛密（上海）健康科技股份有限公司 | Beta-glucan candy tablet with blood sugar and blood pressure reducing health care functions and preparation method thereof |
| CN114343045A (en) * | 2022-01-20 | 2022-04-15 | 艾苛密（上海）健康科技股份有限公司 | Health-care tabletting candy and preparation method thereof |
| CN114560959A (en) * | 2022-03-08 | 2022-05-31 | 湖南朗林生物科技有限公司 | Preparation method of mushroom extract |
| CN114990184A (en) * | 2022-06-16 | 2022-09-02 | 陕西科技大学 | Green co-production preparation method of edible fungus glucan, polypeptide and crude fiber |
-
2008
- 2008-11-10 CN CNA2008101223181A patent/CN101407833A/en active Pending
Cited By (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869264B (en) * | 2010-06-02 | 2012-08-08 | 文承官 | Mushroom concentrated liquid and drink containing beta-glucan and preparation method thereof |
| CN101869264A (en) * | 2010-06-02 | 2010-10-27 | 文承官 | Mushroom concentrated liquid and drink containing beta-glucan and preparation method thereof |
| CN102453105A (en) * | 2010-11-02 | 2012-05-16 | 天津济命生生物科技有限公司 | Extraction method for polysaccharide |
| CN102106875A (en) * | 2010-12-06 | 2011-06-29 | 浙江工业大学 | Method for extracting water-soluble alpha-glucosidase inhibitor |
| CN103059160A (en) * | 2011-10-20 | 2013-04-24 | 中国科学院上海药物研究所 | Beta-glucan GFPBW1, its preparation method and application |
| CN103059160B (en) * | 2011-10-20 | 2015-12-02 | 中国科学院上海药物研究所 | Beta-glucan GFPBW1 and its production and use |
| CN103304680B (en) * | 2012-03-09 | 2017-02-08 | 中国科学院上海药物研究所 | Beta-glucan, and extraction method and application thereof |
| CN103304680A (en) * | 2012-03-09 | 2013-09-18 | 中国科学院上海药物研究所 | Beta-glucan, and extraction method and application thereof |
| CN102603917A (en) * | 2012-03-22 | 2012-07-25 | 无锡德冠生物科技有限公司 | Method for extracting yeast glucosan by utilizing enzymatic method |
| CN103070010A (en) * | 2012-12-27 | 2013-05-01 | 陕西恒田化工有限公司 | Coriolus versicolor strain and method for extracting Coriolus versicolor glucan by utilizing fermentation products of Coriolus versicolor strain |
| CN104277135A (en) * | 2014-09-23 | 2015-01-14 | 华南农业大学 | Pleurotus tuber-regium polysaccharide and extraction method thereof |
| CN105628457A (en) * | 2014-10-27 | 2016-06-01 | 内蒙古蒙牛乳业（集团）股份有限公司 | Exaction method and content determination method of yeast beta-glucan in liquid-state milk |
| CN105628457B (en) * | 2014-10-27 | 2018-05-15 | 内蒙古蒙牛乳业（集团）股份有限公司 | The extracting method and content assaying method of yeast beta-dextran in a kind of liquid milk |
| CN105732250A (en) * | 2016-02-06 | 2016-07-06 | 福建农林大学 | Preparing method for high-purity grifola frondosa polyphenol component |
| CN105732250B (en) * | 2016-02-06 | 2018-03-09 | 福建农林大学 | A kind of preparation method of high-purity grifola frondosus weight polyphenol fraction |
| CN106036846A (en) * | 2016-06-11 | 2016-10-26 | 张俊辉 | Preparation process of agaricus blazei murill and roselle effervescent tablets |
| CN106491662A (en) * | 2016-11-03 | 2017-03-15 | 淳安千岛湖桑都食用菌专业合作社 | A kind of method for cultivating extraction polysaccharide and flavones simultaneously in waste material from Phellinus |
| CN107467128A (en) * | 2017-07-25 | 2017-12-15 | 合肥台香食品有限公司 | A kind of bread of hypoglycemia patient |
| CN110156910A (en) * | 2019-05-10 | 2019-08-23 | 浙江工业大学 | Yeast dextran extract, composition and the application in preparation prevent constipation preparation |
| CN110305920A (en) * | 2019-07-02 | 2019-10-08 | 泉后(广州)生物科技研究院有限公司 | A kind of active fermentation object and its preparation method and application |
| CN113968919A (en) * | 2021-11-25 | 2022-01-25 | 浙江百山祖生物科技有限公司 | Edible fungus extract without auxiliary materials and preparation method thereof |
| CN114292888A (en) * | 2021-12-30 | 2022-04-08 | 赣州禾绿康健生物技术有限公司 | Preparation process of hericium erinaceus extract capable of improving immunity |
| CN114304353A (en) * | 2021-12-30 | 2022-04-12 | 艾苛密（上海）健康科技股份有限公司 | Beta-glucan candy tablet with blood sugar and blood pressure reducing health care functions and preparation method thereof |
| CN114304353B (en) * | 2021-12-30 | 2023-09-19 | 艾苛密（上海）健康科技股份有限公司 | Beta-glucan candy tablet with blood glucose and blood pressure reducing health care function and preparation method thereof |
| CN114343045A (en) * | 2022-01-20 | 2022-04-15 | 艾苛密（上海）健康科技股份有限公司 | Health-care tabletting candy and preparation method thereof |
| CN114343045B (en) * | 2022-01-20 | 2023-11-17 | 河南省寓生堂食养科技有限公司 | Health care tabletting candy and preparation method thereof |
| CN114560959A (en) * | 2022-03-08 | 2022-05-31 | 湖南朗林生物科技有限公司 | Preparation method of mushroom extract |
| CN114560959B (en) * | 2022-03-08 | 2022-12-27 | 湖南朗林生物资源股份有限公司 | Preparation method of mushroom extract |
| CN114990184A (en) * | 2022-06-16 | 2022-09-02 | 陕西科技大学 | Green co-production preparation method of edible fungus glucan, polypeptide and crude fiber |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101407833A (en) | Preparation of edible fungus beta-dextran | |
| CN107858393B (en) | Method for extracting protein polypeptide from walnut meal | |
| CN104921149B (en) | Technology for extracting bran dietary fibers by combining ultrasonic-assisted enzymolysis and microbial fermentation | |
| CN103719880B (en) | Preparation method of high-activity purple sweet potato dietary fiber | |
| CN104187456B (en) | Extract the process of dietary fiber in pears slag | |
| Nwe et al. | Characterization of chitosan and chitosan–glucan complex extracted from the cell wall of fungus Gongronella butleri USDB 0201 by enzymatic method | |
| CN104278066A (en) | Method for preparing wheat bran xylooligosaccharide by superpressure-enzyme combination process | |
| CN104480161A (en) | Ultrafine-grinding assisted enzymatic-hydrolysis based preparation method of wheat bran oligosaccharides | |
| CN103951761A (en) | Method for degrading enteromorpha prolifera polysaccharides by enzymic method | |
| CN101416721A (en) | Method for extracting a great variety of biological active ingredients from dry powder of pasania fungus | |
| CN105384842A (en) | Method for extracting water soluble beta-glucan from sparassis crispa sporophore | |
| CN105384843A (en) | Method for extracting water soluble beta-glucan from inonotus obliquus sporophore | |
| AU2015207336A1 (en) | Process for fractionation of oligosaccharides from agri-waste | |
| KR101345729B1 (en) | Method of extracting arabinoxylan from rice bran | |
| CN104894199B (en) | The synchronic preparation method of one primary yeast small peptide and yeast cell wall polysaccharide | |
| CN101434980B (en) | Preparation of rice bran short peptide | |
| CN100572398C (en) | A kind of preparing mannan | |
| CN104744603A (en) | Preparation method of composition containing [beta]-glucan with basidiomycetes as raw material | |
| CN105481994A (en) | Method for extracting water-soluble beta-glucan from poria cocos fungus entities | |
| CN105463040A (en) | Method for raising yield of xylooligosaccharide | |
| CN114560959B (en) | Preparation method of mushroom extract | |
| CN101235404A (en) | Method for preparing chitosan oligosaccharide with 4-7 polymerization degree | |
| CN102492052A (en) | Method for extracting mannan from waste residue of beer yeast | |
| CN104342475A (en) | Method for preparing glucosaccharase-inhibiting active peptide from hot-extraction peanut pulp | |
| CN107236054A (en) | A kind of preparation method and application of low molecule amount yellow tang glycan |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20090415 |