CN101402916B - Inner hoisting stirrer for cell culture - Google Patents
Inner hoisting stirrer for cell culture Download PDFInfo
- Publication number
- CN101402916B CN101402916B CN 200810202888 CN200810202888A CN101402916B CN 101402916 B CN101402916 B CN 101402916B CN 200810202888 CN200810202888 CN 200810202888 CN 200810202888 A CN200810202888 A CN 200810202888A CN 101402916 B CN101402916 B CN 101402916B
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- CN
- China
- Prior art keywords
- tube
- stirrer
- cell culture
- agitator
- ventpipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/08—Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/02—Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam
Abstract
The invention discloses a cell lift agitator which comprises a separation tube, an exchange tube and a breather tube. The agitator comprises a defoaming cavity and an exhausting funnel; the lower end of the exhausting funnel is communicated with the upper end of the exchange tube and the upper end with the defoaming cavity; the breather tube goes through the defoaming cavity and the exhausting funnel and goes through and is positioned in the exchange tube, which goes through and is positioned in the separation tube; the axial lines of the separation tube, the exchange tube and the breather tube are coincident; and the side wall at the upper end of the separation tube is provided with a centrifuge tube communicated with the separation tube. The exhausting port of the agitator is increased, thus avoiding back pressure caused by the surface tension of a nutrient solution. The lower end of the breather tube of the agitator is provided with a check valve that is of a bicycle valve core structure, has reliable working, not only can allow air intake, but also can prevent a solution from entering the breather tube, thereby putting an end to the possibility that the solution can be pressed in a bearing block. When the agitator is applied to flake fiber carrier fixed bed culture and flake fiber carrier suspension culture, as no free cells are in the solution, the external network of pipes of the breather tube can be removed and breathing is directly carried out in a lift tube so as to form an air lift agitator effect, thus being conducive to the lift of the suspending flake carrier in the lift tube.
Description
Technical field
The present invention relates to a kind of inner hoisting stirrer for cell culture.
Background technology
U.S. NBS company is in the cell lift agitator and the aeration-agitation device of eighties of last century mid-1980s release, as German patent DE 3445952, the pipe latus rectum is too little in the venting port, cause discharge outlet exhaust back pressure increase in the venting cavity, the intraluminal fluid position descends, ventilation bubble " travelling " distance in liquid is too short, has greatly influenced the transmission effect of oxygen." duck chews shape " check valve (Duck Bill Valve) is equipped with on main shaft top, and purpose is in order to prevent that solution from entering the upper axis bearing.And in fact, this " duck chews shape " check valve work is unreliable, and have more than is needed how long (about half a year), solution is pressed into bearing support, causes bearing rust to lose efficacy, and motor can't drive.
Summary of the invention
Technical problem to be solved by this invention provides a kind of inner hoisting stirrer for cell culture, this lift agitator handling ease, compact construction, the transfer rate of raising oxygen.
For solving the problems of the technologies described above, technical scheme provided by the present invention is: a kind of cell lift agitator, include isolated tube, exchange pipe and ventpipe, and this agitator includes froth breaking chamber and exhaust funnel, described exhaust funnel lower end is communicated with exchange pipe upper end, and its upper end is communicated with the froth breaking chamber; Described ventpipe passes the froth breaking chamber and exhaust funnel is located in the exchange pipe, and described exchange pipe is located in the isolated tube; The axial line of described isolated tube, exchange pipe and ventpipe overlaps; Described isolated tube upper end sidewall is provided with centrifuge tube, and described centrifuge tube is communicated with isolated tube.
Described centrifuge tube is provided with 3, and described 3 centrifuge tubes are positioned at sustained height, and 120 degree are provided with each other.
Described centrifuge tube is vertical mutually in the axial direction with isolated tube.
Described exchange pipe is made up of pipe support and screen cloth, and described screen cloth is fixedly connected on the pipe support periphery; Described pipe support side is provided with some through holes, and its bottom is a blind hole.
The bottom of described ventpipe is provided with the check valve of the interior unidirectional conducting outside pipe of pipe.
The outer wall spacing of described exhaust funnel lower end inwall and ventpipe is at least 3mm.
Described check valve is the bicycle valve core structure.
The advantage that the present invention has: the venting port of exhaust funnel strengthens in the agitator, the back pressure of having avoided the nutrient solution surface tension to cause.Ventilation bubble " travelling " distance in nutrient solution strengthens like this, and the residence time increases, and has improved the transmission effect of oxygen.Agitator ventpipe lower end is equipped with the check valve of bicycle valve core structure, reliable operation, but both air inlets, thus can prevent that again solution from entering ventpipe and having stopped the possibility that solution is pressed into bearing support.Ventilation is directly carried out in riser tube, forms the air lift type agitator effect, and the chip carrier that helps to suspend rises in riser tube.
Description of drawings:
Below in conjunction with the drawings and specific embodiments the present invention is described in further detail.
Fig. 1 is a structural representation of the present invention;
Fig. 2 is a partial enlarged drawing among Fig. 1;
Fig. 3 be among Fig. 1 A-A to view
Fig. 4 is a ventpipe bottom enlarged view.
Embodiment:
A kind of inner hoisting stirrer for cell culture shown in Fig. 1,2,3 and 4, include isolated tube 1, exchange pipe 2 and ventpipe 3, this agitator includes froth breaking chamber 9 and exhaust funnel 4, and described exhaust funnel 4 lower ends are communicated with exchange pipe 2 upper ends, and its upper end is communicated with froth breaking chamber 9; Described ventpipe 3 passes froth breaking chamber 9 and exhaust funnel 4 is located in the exchange pipe 2, and described exchange pipe 2 is located in the isolated tube 1; The axial line of described isolated tube 1, exchange pipe 2 and ventpipe 3 overlaps; Described isolated tube 1 upper end sidewall is provided with centrifuge tube 10, and described centrifuge tube 10 is communicated with isolated tube 1.Centrifuge tube 10 is provided with 3, and described 3 centrifuge tubes 10 are positioned at sustained height, and 120 degree are provided with each other.Centrifuge tube 10 is vertical mutually in the axial direction with isolated tube 1.
Exchange pipe 2 is made up of pipe support 21 and screen cloth 22, and described screen cloth 22 is fixedly connected on pipe support 21 peripheries; Described pipe support 21 sides are provided with some through holes 210, and its bottom is a blind hole.
The bottom of ventpipe 3 is provided with the check valve 31 of the interior unidirectional conducting outside pipe of pipe, and this check valve 31 is the bicycle valve core structure, as shown in Figure 4, elasticity epithelium 310 is sealed pore 311, have only when internal pressure during, just can have gas to flow out, and outside liquid can't reflux in the ventpipe greater than the outside.
This cell lift agitator is erected in the jar vertically, liquid floods centrifuge tube 10 in jar, and masterpiece is used for the upper end of ventpipe 3 outside, drives the agitator rotation, because the flowing from bottom to top of the liquid in the centrifugal action isolated tube 1, gone out isolated tube 1 by centrifugal by centrifuge tube 10; And the liquid in exchange pipe 2, the gas that feeds with ventpipe 3 carries out the gas-liquid exchange, constantly discharges from exchange pipe 2 tops, simultaneously, outside liquid constantly passes screen cloth 22 from exchange pipe 2 bottoms and enter inside.Microcarrier in the solution is intercepted at the screen cloth outside.Gas after the exchange rises and enters froth breaking chamber 9 and discharge through exhaust funnel 4.In order to prevent that bubble from blocking the gap between exhaust funnel 4 and the ventpipe 3, so this gap is at least 3mm.
This inner hoisting stirrer for cell culture, applicable to: cell suspension culture, adherent type cell microcarrier (Microcarrier) are cultivated, the sheet-like fiber carrier fixed bed is cultivated, the sheet-like fiber carrier suspension culture.
Claims (7)
1. an inner hoisting stirrer for cell culture includes isolated tube, exchange pipe and ventpipe, and it is characterized in that: this agitator includes froth breaking chamber and exhaust funnel, and described exhaust funnel lower end is communicated with exchange pipe upper end, and its upper end is communicated with the froth breaking chamber; Described ventpipe passes the froth breaking chamber and exhaust funnel is located in the exchange pipe, and described exchange pipe is located in the isolated tube; The axial line of described isolated tube, exchange pipe and ventpipe overlaps; Described isolated tube upper end sidewall is provided with centrifuge tube, and described centrifuge tube is communicated with isolated tube.
2. inner hoisting stirrer for cell culture according to claim 1 is characterized in that: described centrifuge tube is provided with 3, and described 3 centrifuge tubes are positioned at sustained height, and 120 degree are provided with each other.
3. inner hoisting stirrer for cell culture according to claim 1 and 2 is characterized in that: described centrifuge tube is vertical mutually in the axial direction with isolated tube.
4. inner hoisting stirrer for cell culture according to claim 1 is characterized in that: described exchange pipe is made up of pipe support and screen cloth, and described screen cloth is fixedly connected on the pipe support periphery; Described pipe support side is provided with some through holes, and its bottom is a blind hole.
5. inner hoisting stirrer for cell culture according to claim 1 is characterized in that: the bottom of described ventpipe is provided with the check valve of the interior unidirectional conducting outside pipe of pipe.
6. inner hoisting stirrer for cell culture according to claim 1 is characterized in that: the outer wall spacing of described exhaust funnel lower end inwall and ventpipe is at least 3mm.
7. inner hoisting stirrer for cell culture according to claim 5 is characterized in that: described check valve is the bicycle valve core structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810202888 CN101402916B (en) | 2008-11-18 | 2008-11-18 | Inner hoisting stirrer for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810202888 CN101402916B (en) | 2008-11-18 | 2008-11-18 | Inner hoisting stirrer for cell culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101402916A CN101402916A (en) | 2009-04-08 |
| CN101402916B true CN101402916B (en) | 2011-05-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200810202888 Active CN101402916B (en) | 2008-11-18 | 2008-11-18 | Inner hoisting stirrer for cell culture |
Country Status (1)
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| CN (1) | CN101402916B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2474606B1 (en) * | 2009-09-03 | 2019-01-09 | Nikon Corporation | Agitation method, cell culture method, agitation apparatus, and cell culture apparatus |
| CN102618443B (en) * | 2012-03-26 | 2014-09-17 | 上海日泰医药设备工程有限公司 | Microcarrier cell reactor and ventilating pipe thereof |
| CN104195044B (en) * | 2014-09-17 | 2016-10-05 | 上海保兴生物设备工程有限公司 | Bioreactor is centrifuged lift agitator |
| CN105331519A (en) * | 2015-11-27 | 2016-02-17 | 上海日泰医药设备工程有限公司 | Stirring device for fermentation tank |
| CN105331538B (en) * | 2015-11-27 | 2017-09-29 | 上海日泰医药设备工程有限公司 | A kind of cell tank |
| CN105255733B (en) * | 2015-11-27 | 2018-06-19 | 上海日泰医药设备工程有限公司 | A kind of cell tank agitating device |
| CN106119111A (en) * | 2016-08-31 | 2016-11-16 | 上海戈洛思生物科技有限公司 | A kind of cell ventilating net tube |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1124292A (en) * | 1995-11-07 | 1996-06-12 | 许晓增 | Biological reactor for cell culture |
| US5665594A (en) * | 1993-09-09 | 1997-09-09 | Synthecon, Inc. | Gas permeable bioreactor and method of use |
| EP1186653A2 (en) * | 2000-08-18 | 2002-03-13 | Levitronix LLC | Method to keep an article in suspension and bioreactor based thereon |
| CN201309941Y (en) * | 2008-11-18 | 2009-09-16 | 上海日泰医药设备工程有限公司 | Lift agitator in cell culture fluid |
-
2008
- 2008-11-18 CN CN 200810202888 patent/CN101402916B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5665594A (en) * | 1993-09-09 | 1997-09-09 | Synthecon, Inc. | Gas permeable bioreactor and method of use |
| CN1124292A (en) * | 1995-11-07 | 1996-06-12 | 许晓增 | Biological reactor for cell culture |
| EP1186653A2 (en) * | 2000-08-18 | 2002-03-13 | Levitronix LLC | Method to keep an article in suspension and bioreactor based thereon |
| CN201309941Y (en) * | 2008-11-18 | 2009-09-16 | 上海日泰医药设备工程有限公司 | Lift agitator in cell culture fluid |
Non-Patent Citations (2)
| Title |
|---|
| 王树君等.用微载体系统培养 Vero 细胞生产高滴度狂犬病毒液.《中国生物制品学杂志》.2004,第 17 卷(第 6 期),380-382. * |
| 胡显文等.新型气液双升式动物细胞反应器.《生物技术通讯》.1999,第 10 卷(第 2 期),100-103. * |
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| Publication number | Publication date |
|---|---|
| CN101402916A (en) | 2009-04-08 |
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