CN101385640A - Anatomy image forming method and system - Google Patents

Anatomy image forming method and system Download PDF

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Publication number
CN101385640A
CN101385640A CNA2008101971977A CN200810197197A CN101385640A CN 101385640 A CN101385640 A CN 101385640A CN A2008101971977 A CNA2008101971977 A CN A2008101971977A CN 200810197197 A CN200810197197 A CN 200810197197A CN 101385640 A CN101385640 A CN 101385640A
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China
Prior art keywords
grinding
sample
plane
imaging
image
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CNA2008101971977A
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Chinese (zh)
Inventor
龚辉
刘谦
骆清铭
白雪岭
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Huazhong University of Science and Technology
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Huazhong University of Science and Technology
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Priority to CNA2008101971977A priority Critical patent/CN101385640A/en
Publication of CN101385640A publication Critical patent/CN101385640A/en
Pending legal-status Critical Current

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Abstract

The invention provides a fault imaging system. The system comprises a fault grinding device which is provided with a worktable and a grinding cutter and is used to form a grinding plane on the sample fixed on the worktable by the grinding cutter, an image collection device which is used to carry out color imaging on the grinding plane formed on the sample, a control unit which is connected with the fault grinding device and the image collection device respectively by means of wired or wireless connection and controls the fault grinding device and the image collection device to operate alternatively, so as to form the grinding plane alternatively and image the formed grinding plane, and a storage unit which is used to store the imaging information of color images obtained by the image collection device. The invention also provides a corresponding fault imaging method.

Description

The anatomy image forming system and method
Technical field
The present invention relates to imaging system, relate more specifically to a kind of anatomy image forming system and method that is applicable to small animal research.
Background technology
Along with development of life science, to gather biological 26S Proteasome Structure and Function information and carry out the integration that digitized helps biomedical sector information and share, this type of work has become a big focus of domestic and international research.In view of the experiment toy has become important study model in research fields such as disease and drug developments, setting up a cover and can obtain the experiment toy imaging system of the dissection tomography structural information of organ in whole or in part with high spatial resolution, will be favourable for the medical information analysis meeting of obtaining or testing toy of testing toy anatomical data collection.
Obtaining when dissecting faultage image, adopt microtome to sample section to use the micro imaging method of microscope imaging to be only applicable to size then less, the biological sample of character homogeneous (for example embryo, number brains, cochlea etc.).Because slicing processes and imaging process is separate carries out, thereby may destroy the three-dimensional space position of section, and slicing processes need expend a large amount of manpowers, the image acquisition time cycle is also longer; If adopt optical imaging methods such as optical sectioning imaging or NMR (Nuclear Magnetic Resonance)-imaging, the faultage image that then obtains will be lost the original colouring information of biological sample, and this is unfavorable for the identification and the boundary demarcation of biological different tissues organ.
Therefore, need a kind ofly can to carry out the grinding of small size animal sample and the anatomy image forming system and method for fault imaging immediately, more easily obtain chromatic dissection faultage image information with faster.
Summary of the invention
In view of this, the invention provides a kind of computed tomography (SPECT) system and method, can carry out grinding successively and grinding planar imaging immediately continuously the atom test sample, thus the faster color image information that more easily obtains institute's grinding fault plane.
The invention provides a kind of computed tomography (SPECT) system, it comprises:
The tomography grinding attachment, it has workbench and grinding knife tool, is used for by described grinding knife tool forming on the sample that is fixed on the described workbench grinding plane;
Image collecting device is used for colour imaging is carried out on the grinding plane that forms on the described sample;
Control unit, it is connected respectively to described tomography grinding attachment and described image collecting device by wired or wireless connected mode, and this control unit is controlled described tomography grinding attachment and described image collecting device blocked operation alternately to form described grinding plane and imaging is carried out on formed grinding plane; With
Storage device is used to store the colour imaging image information of being obtained by described image collecting device.
Described tomography grinding attachment can be lathe, milling machine, grinding machine or planer.
Described tomography grinding attachment can be CNC milling machine, and described grinding knife tool is face milling cutters.
Described image collecting device can be color scanner or camera.
The imaging plane of described image collecting device can be between described image collecting device and described workbench, and is parallel to described grinding plane.
Described control unit can be computer.
Described storage device can be arranged in described image collecting device or the described control unit, perhaps is connected to described image collecting device or described control unit.
When described sample adopted freezing embedding treatment, described tomography grinding attachment and described image collecting device can be placed under-10 ± 4 degrees centigrade the temperature.
The present invention also provides a kind of tomograph imaging method, and this method comprises:
A, sample is fixed;
B, on described sample, form the grinding plane;
C, colour imaging is carried out on described grinding plane, the storage color image-forming information returns execution in step B then.
Can form described grinding plane by lathe, milling machine, grinding machine or planer.
Can carry out colour imaging by color scanner or camera.
Can alternately carry out by computer controlled step B and step C.
Can adopt freezing embedding treatment to described sample, then described formation grinding plane can be carried out under-10 ± 4 degrees centigrade temperature with the step of carrying out colour imaging.
In step C, can further comprise: if the quantity of described colour imaging information reaches predetermined value or described sample thickness less than predetermined value, end operation then.
As from the foregoing, anatomy image forming system and method provided by the invention is simple and convenient, with low cost, sample is carried out the sample fault plane that continuous grinding successively obtains different depth, and each fault plane carried out the white light colour imaging, obtain experiment atom internal anatomy tomography structural information continuously with high spatial resolution, thereby the dissection tomography coloured image that can in extremely rapid succession realize the small size biological sample is automatically gathered, be not only applicable to the digitized data acquisition platform of toy anatomical structure, also can be used as the pathological analysis means of toy or animal tissue's organ and be applied in the work such as medical research.
Description of drawings
Fig. 1 is the structure chart according to zootomy computed tomography (SPECT) system of the present invention.
Fig. 2 is the flow chart according to zootomy tomograph imaging method of the present invention.
The specific embodiment
By anatomy image forming system and method provided by the invention, by biological sample is alternately moved between grinding position and image space, realization is to the grinding successively and the anatomy image forming continuously immediately of biological sample, to obtain chromatic dissection faultage image information.
For making the purpose, technical solutions and advantages of the present invention clearer, the present invention is described in further detail below in conjunction with accompanying drawing.
As shown in Figure 1, anatomy image forming provided by the invention system comprises: tomography grinding attachment 1, image collecting device 2, and the control unit 3 that links to each other with image collecting device 2 with tomography grinding attachment 1 respectively, wherein, tomography grinding attachment 1 has workbench 4 and grinding knife tool 6, and image collecting device 2 is within the moving range of workbench 4, and, the imaging plane of image collecting device 2 be parallel to the grinding plane of tomography grinding attachment 1 and be in image collecting device 2 and workbench 4 between.Like this, 1 pair of sample 8 that is fixed on the workbench 4 of control unit 3 control tomography grinding attachments carries out surfacing, and fault imaging is carried out on the surface of 2 pairs of samples 8 of image collecting device then, and the faultage image information transmission that obtains can be arrived control unit 3.
In one embodiment, tomography grinding attachment 1 can be milling machine, and for example three shaft vertical CNC milling machine are used for sample 8 is carried out surfacing.In this case, tomography grinding attachment 1 (being milling machine 1) can comprise: workbench 4, and milling machine fuselage 5, face milling cutters 6 and digital control system 7, and preferably adopt large diameter plain milling cutter to cut planar crudy to guarantee sample mill.In addition, what can expect is that tomography grinding attachment 1 also can be other machined grinding attachments, for example lathe, planer, grinding machine.
In one embodiment, image collecting device 2 can be color scanner, for example, and coloured plate formula scanner, be used for gathering anatomic tissue tomography coloured image, and control unit 3 is stored or be sent to the color image information that gets access to from the grinding plane of sample 8.In one embodiment, the imaging plane of scanner 2 is between the workbench 4 of scanner 2 and CNC milling machine 1, that is, scanner 2 is towards workbench 4, the imaging plane of scanner 2 is parallel to the milling plane and the workbench 4 of milling machine 1, and perpendicular to the vertical Z axle of milling machine 1.But, in other embodiments, the imaging plane of scanner 2 also can not exclusively be arranged in parallel, but as required or allow to exist the angle of inclination, and regulate by software or hardware mode when needed.Parameters such as the scanning breadth of scanner 2 and resolution can be determined by the size and the user's request of sample imaging surface, for example, maximum imaging region with scanner of A4 breadth can be 297 * 210mm, and resolution is that the imaging capability of the scanner of 4800dpi can reach 5 microns of every pixels.For realizing the milling machine that the Z axle moves by travelling table 4, scanner 2 can be fixed on milling machine shell or the ground; For realizing the milling machine that the Z axle moves by mobile milling cutter 6, scanner 2 can be fixed on the milling machine shell that together moves with milling cutter 6.In addition, in another embodiment, image collecting device 2 also can be other coloured image imaging device, for example cameras.
Control unit 3 can comprise signal processor, perhaps can be ordinary individual's computer (PC), is used to control the operation of tomography grinding attachment 1 and image collecting device 2.Further, control unit 3 can have memory function or comprise memorizer, be used to store the faultage image information of being gathered by image collecting device 2, perhaps, control unit 3 also can send to the faultage image information of receiving from image collecting device 2 other external memory and store.The external control port that control unit 3 can be connected to tomography grinding attachment 1 by cable is with the grinding of control sample surfaces, and this type of cable can be determined by the provider of the numerical control module of tomography grinding attachment 1; Control unit 3 can be connected to image collecting device 2 by cable and gather and the transmission faultage image data with control image collecting device 2, and this type of cable can be determined by the provider of image collecting device 2.But, what can expect is that control unit 3 also can be connected to tomography grinding attachment 1 and image collecting device 2 by other wired modes or wireless mode.In addition, when control unit 3 is PC 3, it can will be connected to image collecting device 2 (for example scanner 2) by the USB mode.
In one embodiment, anatomy image forming provided by the invention system comprises: CNC milling machine 1, scanner 2 and as the PC 3 of control unit.Below will describe system operation methods provided by the invention in detail with reference to Fig. 2 as example.But, it should be understood that and adopt other tomography grinding attachments 1 that the anatomy image forming system of image collecting device 2 and control unit 3 also can realize following operation.
When carrying out anatomy image forming, at first will be fixed in after sample 8 embeddings on the workbench 4 of CNC milling machine 1; Adopt manual mode or make sample 8 aim at and on sample 8, mill out the grinding plane in (that is grinding position) below the milling cutter 6 by the numerical control program automated manner; Then, sample 8 (that is, image space) below scanner 2 is aimed at and made the grinding plane of sample 8 be in the imaging plane place of scanner 2, scanning imagery is carried out on 2 pairs of samples of scanner, 8 grinding planes; After scanning imagery is finished the image that obtains is transferred to PC 3 and stores from scanner 2.
After the first time, scanning imagery was finished, sample 8 leaves the image space of scanner 2 belows and returns the grinding position of milling cutter 6 belows and increase an amount of feeding Δ Z in Z-direction, wherein Δ Z be faultage image at interval, the at the uniform velocity fault plane that makes new advances of milling, i.e. second grinding plane then; Then, sample 8 moves to the image space of scanner 2 belows again and makes second grinding plane of sample 8 be in the imaging plane place of scanner 2, and scanning imagery is carried out on 8 second grinding planes of 2 pairs of samples of scanner; After for the second time scanning imagery is finished, the image that obtains is transferred to PC 3 and stores from scanner 2.Like this, cycling in the above described manner, sample 8 carries out the grinding planar imaging on formation grinding plane of the grinding position below the milling cutter 6 and the image space below scanner 2, these two kinds of operations hocket, immediately formed grinding plane is carried out imaging and the faultage image that obtains can be transferred to PC 3 storing, up to reaching default termination condition, for example, the quantity of the image-forming information that is obtained reaches the residual thickness of predetermined value or sample 8 less than predetermined value, then end operation.
In the aforesaid operations process, the position of sample 8 can utilize the coordinate system of CNC milling machine 1 to carry out record, maybe can take to redefine the method at user coordinates zero point and come record.For example, the image space of sample 8 below scanner 2 can be recorded as (XO, YO, Z), the grinding position of sample 8 below milling cutter 6 can be recorded as (XO ', YO ', Z '), when sample 8 between image space and grinding position during positioned alternate, its XY position (XO, YO) and (XO ', YO ') remain unchanged, and its position Z and Z ' along the Z axle correspondingly changes adjustment with each required Z axle amount of feeding Δ Z, to satisfy the needs of flat surface grinding operation and scanner imaging operation.Wherein, the each required amount of feeding Δ Z of Z axle equals faultage image at interval, and it can determine that for example, when the space requirements between the anatomy image forming plane was 10 microns, Δ Z was set at 10 microns according to user's request.Δ Z is set at constant usually, but also can be set to variable when needed.
In the above description, sample 8 can move at the image space below the scanner 2 with between the grinding position below the milling cutter 6.But, what can expect is, described " top " and " below " only are exemplary description, in other embodiments, sample 8 also can be aimed to carry out respective imaging operation or grinding action in the image space or the grinding position on image collecting device 2 (for example scanner 2) or grinding knife tool 6 (for example milling cutter 6) " left " or " right-hand ".And, be also contemplated that, described sample 8 " moving " between image space and grinding position can be to relatively move, for example, when sample 8 " moves to " image space of image collecting device 2 (for example scanner 2) below, both can keep image collecting device 2 static and mobile example 8 also can keep sample 8 static and mobile image collecting devices 2.
According to above-described operation, can determine the working procedure of CNC milling machine 1 and write numerical control program, to carry out sample 8 automatically at image space (XO, YO, Z) with grinding position (XO ', YO ', moving back and forth Z '), and increase the amount of feeding Δ Z of Z-direction successively.
In above operation, the speed of mainshaft of CNC milling machine 1 can be set at 2000 rev/mins, and the feed speed when sample 8 carries out milling can be set at 600 mm/min.And these two parameters can be adjusted as required, for example adjust according to employed sample embedded material, thereby make the sample mill after the milling cut planar surface roughness minimum, are beneficial to imaging.
In one embodiment, can write out numerical control program to automatically perform above-mentioned operation by CNC milling machine 1.PC 3 as control unit sends the start-up routine order to the numerical control module of CNC milling machine 1, start the numerical control program of being write, CNC milling machine 1 will mill out the grinding plane successively according to the numerical control program start-up operation and on the sample 8 that is in grinding position, and afterwards sample 8 is moved to image space and carry out fault imaging by scanner 2, scanner 2 scanning tomographies also store faultage image among the PC 3 into.After imaging was finished each time, PC 3 sent to CNC milling machine 1 and restarts order with next grinding plane of milling, and afterwards fault imaging is carried out on the grinding plane, and so circulation is up to the tomography grinding of finishing whole sample 8 and imaging work.Wherein, can start the line scanning preview of going forward side by side of scan control software among scanner 2 or the PC 3 when fault imaging, that is, 8 position and big or small frames in preview area are selected scanning area and this scanning area are carried out image scanning per sample.The time of scanning preview also can be provided with when writing above-mentioned numerical control program.
In one embodiment, PC 3 sends control command and scanner uni to CNC milling machine 1 and preserves the process of image and can realize by Automatic Program.For example, can easily realize 3 pairs of CNC milling machine 1 Long-distance Control of PC or directly control by DNC (DNC) mode; For example, adopt the image management software among the PC3 can realize scanner 2 continuous acquisition images and the preservation of image file automatic numbering in conjunction with the scanner driving.In addition, can adopt the keyboard logging software of PC 3 or coding to realize that CNC milling machine 1 is carried out milling and scanner 2 carries out the co-ordination of image acquisition.
In addition, the embedding mode of sample 8 per sample organizational composition and desired realization ground effect and adopt resin embedding, modes such as paraffin embedding or freezing embedding.When adopting the mode of freezing embedding, tomography grinding attachment 1 (for example CNC milling machine 1) and image collecting device 2 (for example scanner 2) need place freezer 9, and the temperature of freezer 9 is set at-10 ± 4 degrees centigrade.
As from the foregoing, anatomy image forming system and method provided by the invention is simple and convenient, with low cost, sample is carried out the sample fault plane that continuous grinding successively obtains different depth, and each fault plane carried out the white light colour imaging, obtain experiment atom internal anatomy tomography structural information continuously with high spatial resolution, thereby the dissection tomography coloured image that can in extremely rapid succession realize the small size biological sample is automatically gathered, be not only applicable to the digitized data acquisition platform of toy anatomical structure, also can be used as the pathological analysis means of toy or animal tissue's organ and be applied in the work such as medical research.

Claims (14)

1, a kind of computed tomography (SPECT) system is characterized in that, this system comprises:
The tomography grinding attachment, it has workbench and grinding knife tool, is used for by described grinding knife tool forming on the sample that is fixed on the described workbench grinding plane;
Image collecting device is used for colour imaging is carried out on the grinding plane that forms on the described sample;
Control unit, it is connected respectively to described tomography grinding attachment and described image collecting device by wired or wireless connected mode, and this control unit is controlled described tomography grinding attachment and described image collecting device blocked operation alternately to form described grinding plane and imaging is carried out on formed grinding plane; With
Storage device is used to store the colour imaging image information of being obtained by described image collecting device.
2, system according to claim 1 is characterized in that,
Described tomography grinding attachment is lathe, milling machine, grinding machine or planer.
3, system according to claim 1 is characterized in that,
Described tomography grinding attachment is a CNC milling machine, and described grinding knife tool is face milling cutters.
4, according to the described system of aforementioned arbitrary claim, it is characterized in that,
Described image collector is changed to color scanner or camera.
5, according to the described system of aforementioned arbitrary claim, it is characterized in that,
The imaging plane of described image collecting device and is parallel to described grinding plane between described image collecting device and described workbench.
6, according to the described system of aforementioned arbitrary claim, it is characterized in that,
Described control unit is a computer.
7, according to the described system of aforementioned arbitrary claim, it is characterized in that,
Described storage device is arranged in described image collecting device or the described control unit, perhaps is connected to described image collecting device or described control unit.
8, according to the described system of aforementioned arbitrary claim, it is characterized in that,
When described sample adopted freezing embedding treatment, described tomography grinding attachment and described image collecting device were placed under-10 ± 4 degrees centigrade the temperature.
9, a kind of tomograph imaging method is characterized in that, this method comprises:
A, sample is fixed;
B, on described sample, form the grinding plane;
C, colour imaging is carried out on described grinding plane, the storage color image-forming information returns execution in step B then.
10, method according to claim 9 is characterized in that,
Form described grinding plane by lathe, milling machine, grinding machine or planer.
11, according to claim 9 or 10 described methods, it is characterized in that,
Carry out colour imaging by color scanner or camera.
12, according to each described method in the claim 9-11, it is characterized in that,
Alternately carry out by computer controlled step B and step C.
13, according to each described method in the claim 9-12, it is characterized in that,
Described sample adopts freezing embedding treatment, and carry out under-10 ± 4 degrees centigrade temperature with the step of carrying out colour imaging on described formation grinding plane.
14, according to each described method in the claim 9-13, it is characterized in that,
In step C, further comprise: if the quantity of described colour imaging information reaches predetermined value or described sample thickness less than predetermined value, end operation then.
CNA2008101971977A 2008-10-08 2008-10-08 Anatomy image forming method and system Pending CN101385640A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105891208A (en) * 2016-04-13 2016-08-24 华中科技大学 Method for obtaining cytoarchitectonic information for establishing standard map data set
CN107181931A (en) * 2017-05-15 2017-09-19 华中科技大学 A kind of camera system for orthogonal turning and milling process high speed imaging
CN107315009A (en) * 2017-07-07 2017-11-03 重庆大学 The tomography digital imaging devices and three-dimensional numerical value Reverse reconstruction method of indoor soil-rock mixture sample

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105891208A (en) * 2016-04-13 2016-08-24 华中科技大学 Method for obtaining cytoarchitectonic information for establishing standard map data set
CN105891208B (en) * 2016-04-13 2019-03-26 华中科技大学 For constructing the architecture cytoarchitectonic information acquisition method of standard diagram data set
CN107181931A (en) * 2017-05-15 2017-09-19 华中科技大学 A kind of camera system for orthogonal turning and milling process high speed imaging
CN107315009A (en) * 2017-07-07 2017-11-03 重庆大学 The tomography digital imaging devices and three-dimensional numerical value Reverse reconstruction method of indoor soil-rock mixture sample
CN107315009B (en) * 2017-07-07 2019-10-29 重庆大学 The fault imaging device and three-dimensional numerical value Reverse reconstruction method of soil-rock mixture sample

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