CN101380053A - Production method of white lower-fat salt-free edible silkworm protein powder - Google Patents
Production method of white lower-fat salt-free edible silkworm protein powder Download PDFInfo
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- CN101380053A CN101380053A CNA2007101314082A CN200710131408A CN101380053A CN 101380053 A CN101380053 A CN 101380053A CN A2007101314082 A CNA2007101314082 A CN A2007101314082A CN 200710131408 A CN200710131408 A CN 200710131408A CN 101380053 A CN101380053 A CN 101380053A
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Abstract
The invention discloses a production method of a white low-fat odorless and salt-free edible silkworm pupa protein powder. The method comprises the following procedures: fresh wet or dry silkworm pupa, tussah pupa or caster silkworm pupa is excorticated after being broken and separated and degreased after being dissolved in alkali solution; then the acid sinking is configurated and membranal filtrated to desalted and dewatered, and is alkali dissolved again and then the hydrogen peroxide or the ozone is added to decolorize and deodorize; then the membrane filtration is adopted for dewatering; then the ethanol is used for immersing and cleaning, degreasing, dewatering, decolorizing and deodorizing the protein; the protein is then superfinely comminuted after being vacuum dried, deodorized and dewatered, thus the white low-fat and salt-free edible silkworm pupa protein powder without the pupa smell is obtained. In the method, the effects of decolorizing, deodorizing and desalting are good and the products have a high quality; the amino acid composition is of equilibrium; the protein content reaches more than 90 percent, the content of the fat is lower than 2 percent and the ash content is less than 3 percent; the products have a good safety without organic residues, thus can be applied to the industries of medicine and food. At the same time, by adopting the method, the comprehensive cost is low, the auxiliary materials for production can be reused without pollution caused by the discharged acid waste water; the chosen equipment is all suitable for continuous mass production.
Description
Technical field
The invention belongs to the technical field of producing of pupa albumen.
Technical background
China is a sericulture big country, produces 650000 tons of silk cocoons per year, accounts for 70% of the total cocoon amount in the world, and wherein bright silkworm chrysalis output can reach 500,000 tons.Silkworm chrysalis has high nutritive value, contains rich in protein (bright silkworm chrysalis contains crude protein and accounts for 51%), aliphatic acid (crude fat accounts for 29%), vitamin (comprising vitamin A, vitamin B2, vitamin D and ergosterol etc.).The protein content of silkworm chrysalis is higher than normal food far away more than 50%, and the essential amino acid A wide selection of colours and designs in the protein, and silkworm chrysalis protein is made up of 18 seed amino acids, and wherein 8 seed amino acid content of needed by human are very high.The amino acid content of 8 kinds of needed by human of this in the silkworm chrysalis approximately is 2 times of pork, 4 times of egg, 10 times of milk, the amino acid nutrient equilibrium of 8 kinds of needed by human, ratio is suitable, meeting the requirement of FAO/WHO (FAO (Food and Agriculture Organization of the United Nation) and the World Health Organization), be fit to very much the needs of human body, is a kind of insect protein of high-quality.Silkworm chrysalis also is unique insects food in Ministry of Public Health's approval " as the new resource for food list of bread and cheese management ".
Fresh silkworm chrysalis fat content height, and unpleasant stench flavor arranged, therefore to produce the high-quality silkworm chrysalis protein that reaches the food grade standard, problems such as flavor degreasing desalination are taken off in the decolouring that must resolve in the production process, for a long time, the numerous researchers and the producer have carried out useful exploration, but exist following problem with regard to the production technology of having announced at present: 1, product quality is poor.As with the degreasing silkworm chrysalis being raw material production edible silkworm albumen powder, the problem of degreasing is to have solved, but because before the degreasing, seldom silkworm chrysalis is carried out processing such as removal of impurities, what perhaps have goes bad owing to standing time is long, and impurity is many in the degreasing silkworm chrysalis, the dead pupa of dead silkworm is many, and rotten pupa is many, so, be the silkworm chrysalis protein powder poor quality of producing as raw material with it.2, output is restricted.Have invention to claim to produce the silkworm chrysalis protein powder with the method for supercritical extract, this method investment is big, and production capacity is little, can not form suitability for industrialized production.3, the organoleptic indicator of product is poor.If any only with hydrogen peroxide or the ozone flavor that decolours, what have only carries out degreasing with organic solvent and takes off flavor owing to there is not the method for comprehensive degreasing deodorization decoloring, effect is all undesirable.
Summary of the invention
For overcoming the shortcoming that the affair prior art exists, the invention provides a kind of production method of white lower-fat salt-free edible silkworm protein powder, the silkworm chrysalis protein powder quality better that this production method is produced, amino acid are formed balanced, no organic residual, the protein content height, fat content is low, production cost is low, and technical process is pollution-free, is suitable for large-scale production.
The present invention solves the problems of the technologies described above the technical scheme of being taked to be: a kind of production method of white lower-fat salt-free edible silkworm protein powder, it comprises the steps:
(1) select pupa to pulverize: get fresh wet, dried mulberry silkworm chrysalis, tussah chrysalis or castor-oil plant silkworm chrysalis, reject dead silkworm, stiff silkworm, bad pupa and other fiber impurities after, mechanical crushing;
(2) degreasing: the material of pulverizing is put into dilute alkaline soln dissolve, saponification part grease, and filter out puparium;
(3) first separation: will filter solution separate with two-phase butterfly seperator, remove chitin and saponified oil fat granule in the solution, separate grease in the pupa albumen liquid with three-phase butterfly seperator again;
(4) once transfer isoelectric point: the protein liquid that separates is regulated isoelectric point and precipitation with acid, and with spiral separator to the protein solution that transfers to isoelectric point carry out albumen and moisture from; The aqueous solution employing that separates is filtered, and a small amount of albumen in the recycle-water solution, the aqueous solution after the filtration are treated down to criticize when producing and are reused;
(5) decoloration and deodorization: the albumen that makes is added dilute alkali once more after the dissolving, add hydrogen peroxide or ozone and carry out decoloration and deodorization;
(6) secondary is transferred isoelectric point: the protein liquid behind the decoloration and deodorization is regulated isoelectric point and precipitation with acid once more, then with spiral separator carry out albumen and moisture from;
(7) decolouring degreasing dehydration deodorization: the albumen that makes is added ethanol embathe, the degreasing of further decolouring dehydration deodorization;
(8) secondary separation:, simultaneously ethanol is reclaimed with horizontal spiral seperator protein isolate and ethanol;
(9) drying and crushing: the albumen that separates is carried out vacuum drying, and further deodorization and dehydration, ultramicro grinding make finished product.
In the above-mentioned steps (2), the temperature of saponification solution is 50-70 ℃, pH value 9-11.
The ethanol of employing 95% embathes in the above-mentioned steps (7), and the temperature of ethanol is 40-60 ℃.
Add hydrogen peroxide in the above-mentioned steps (5) or ozone carries out decoloration and deodorization, temperature is 50-70 ℃
Two-phase butterfly seperator described in the above-mentioned steps (5) is a solid-liquid two-phase butterfly seperator, and described three-phase butterfly seperator is liquid-liquid-solid three-phase butterfly seperator
Spiral separator described in the above-mentioned steps (6) is the horizontal spiral seperator.
The present invention compared with prior art has following advantage:
The one, guarantee that raw material is fresh.Have only raw material fresh, just might produce high-quality silkworm chrysalis protein powder.The present invention is adapted at the production that the silkworm chrysalis protein powder is carried out in the raw material production base most, produces with the wet silkworm chrysalis behind reeling mill's filature on the same day, and raw material does not need oven dry, directly pulverizes, and has both kept product quality, has saved production cost again; After rejecting dead silkworm, stiff silkworm, bad pupa and the oven dry of other fiber impurities, the not long fresh dried pupa of storage time also can be used as raw material production.
The 2nd, the degreasing deodorization decoloring is thorough.Through three degreasings, three deodorizations, twice decolouring, the integrated use several different methods guarantees that the organoleptic indicator of product reaches the food grade standard in the production process.
The 3rd, product yield height.Adopt ceramic membrane filter, reduced the loss of proteins in the production process.
The 4th, production technology is simple, the equipment less investment, and all are common apparatus, can carry out large-scale production.
The 5th, production auxiliary materials is recycling, has both saved production cost, has also reduced the discharging of pollutant simultaneously.
Specific embodiment:
Get the fresh wet silkworm chrysalis of reeling mill, behind rejecting dead silkworm, stiff silkworm, bad pupa and other fiber impurities, weigh 100 kilograms and carry out mechanical crushing; The material pulverized added in the dilute alkaline soln in 1 to 1.8 ratio dissolve, the pH value of dilute alkaline soln is 9 to 11, and temperature is 60 to 70 to spend dissolution time 50 to 70 minutes;
Filter the elimination puparium with the stainless (steel) wire about 10 orders; With filter solution with two butterfly seperators of solid-liquid branch leave away residual puparium and saponified oil fat granule in the solution; Separate the grease in the pupa albumen liquid with liquid-liquid-solid three butterfly seperators again;
The protein liquid that separates is regulated isoelectric point and precipitation with watery hydrochloric acid, pH value between 4.3 to 4.6, and with the horizontal spiral seperator to the protein solution that transfers to isoelectric point carry out albumen and moisture from; Adopt ceramic membrane to filter to the aqueous solution that separates, a small amount of albumen in the recycle-water solution, the aqueous solution after the filtration are treated down to criticize when producing and are reused;
The wet albumen that makes is added dilute alkali once more after the dissolving, heat to 60 degree and carry out decoloration and deodorization, two hours time in 100 to 2 ratio adding hydrogen peroxide; Protein liquid behind the decoloration and deodorization is regulated isoelectric point and precipitation with acid once more, then with the horizontal spiral seperator carry out albumen and moisture from;
The wet albumen that makes is added 95% ethanol by 1 to 1 embathe, temperature is increased to 60 degree, and insulation was embathed 30 minutes, the degreasing of further decolouring dehydration deodorization; Use horizontal spiral seperator protein isolate and ethanol then, with alcohol recovery device ethanol is reclaimed simultaneously;
The albumen that separates is carried out vacuum drying, further deodorization and dehydration, temperature is controlled at 60 to 70 degree, and vacuum 0.08 is to 0.1MPa; Pupa albumen behind the vacuum drying deodorization and dehydration is carried out ultramicro grinding, and the order number makes 12.8 kilograms of finished products between 200 to 300 orders.The color of product is shallow white, free from extraneous odour, taste no bitter taste, after measured, protein content (butt) reaches 90.2%, and fat content is 1.8%, the ash content of coal 2.8%, product P H value 5.2, sanitary index such as content of beary metal such as lead, inorganic arsenic, total mercury and total number of bacteria, yeast and mould, coliform, pathogenic bacteria all meets the state food specified standard.
Claims (6)
1, a kind of production method of white lower-fat salt-free edible silkworm protein powder is characterized in that: comprise the steps:
(1) select pupa to pulverize: get fresh wet, dried mulberry silkworm chrysalis, tussah chrysalis or castor-oil plant silkworm chrysalis, reject dead silkworm, stiff silkworm, bad pupa and other fiber impurities after, mechanical crushing;
(2) degreasing: the material of pulverizing is put into dilute alkaline soln dissolve, saponification part grease, and filter out puparium;
(3) first separation: will filter solution separate with two-phase butterfly seperator, remove chitin and saponified oil fat granule in the solution, separate grease in the pupa albumen liquid with three-phase butterfly seperator again;
(4) once transfer isoelectric point: the protein liquid that separates is regulated isoelectric point and precipitation with acid, and with spiral separator to the protein solution that transfers to isoelectric point carry out albumen and moisture from; The aqueous solution employing that separates is filtered, and a small amount of albumen in the recycle-water solution, the aqueous solution after the filtration are treated down to criticize when producing and are reused;
(5) decoloration and deodorization: the albumen that makes is added dilute alkali once more after the dissolving, add hydrogen peroxide or ozone and carry out decoloration and deodorization;
(6) secondary is transferred isoelectric point: the protein liquid behind the decoloration and deodorization is regulated isoelectric point and precipitation with acid once more, then with spiral separator carry out albumen and moisture from;
(7) decolouring degreasing dehydration deodorization: the albumen that makes is added ethanol embathe, the degreasing of further decolouring dehydration deodorization;
(8) secondary separation:, simultaneously ethanol is reclaimed with horizontal spiral seperator protein isolate and ethanol;
(9) drying and crushing: the albumen that separates is carried out vacuum drying, and further deodorization and dehydration, ultramicro grinding make finished product.
2, the production method of white lower-fat salt-free edible silkworm protein powder according to claim 1 is characterized in that: in the above-mentioned steps (2), the temperature of saponification solution is 50-70 ℃, pH value 9-11.
3, the production method of white lower-fat salt-free edible silkworm protein powder according to claim 1 is characterized in that: the ethanol of employing 95% embathes in the above-mentioned steps (7), and the temperature of ethanol is 40-60 ℃.
4, the production method of white lower-fat salt-free edible silkworm protein powder according to claim 1 is characterized in that: add hydrogen peroxide in the above-mentioned steps (5) or ozone carries out decoloration and deodorization, temperature is 50-70 ℃
5, the production method of white lower-fat salt-free edible silkworm protein powder according to claim 1, it is characterized in that: the two-phase butterfly seperator described in the above-mentioned steps (5) is a solid-liquid two-phase butterfly seperator, and described three-phase butterfly seperator is liquid-liquid-solid three-phase butterfly seperator.
6, the production method of white lower-fat salt-free edible silkworm protein powder according to claim 1 is characterized in that: the spiral separator described in the above-mentioned steps (6) is the horizontal spiral seperator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101314082A CN101380053A (en) | 2007-09-07 | 2007-09-07 | Production method of white lower-fat salt-free edible silkworm protein powder |
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| CNA2007101314082A CN101380053A (en) | 2007-09-07 | 2007-09-07 | Production method of white lower-fat salt-free edible silkworm protein powder |
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| CN101380053A true CN101380053A (en) | 2009-03-11 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103288946A (en) * | 2013-05-17 | 2013-09-11 | 广西大学 | Preparation method of white, low-fat and odorless silkworm pupa protein |
| CN104286846A (en) * | 2014-09-22 | 2015-01-21 | 广东省农业科学院蚕业与农产品加工研究所 | Preparation method of silkworm chrysalis food with alcohol flavor |
| CN104304639A (en) * | 2014-09-22 | 2015-01-28 | 广东省农业科学院蚕业与农产品加工研究所 | Preparation method of concentrated silkworm chrysalis protein powder without abnormal smell |
| CN105341630A (en) * | 2015-12-14 | 2016-02-24 | 南通那芙尔服饰有限公司 | Preparation method for deodorized silkworm pupae |
| CN106578602A (en) * | 2016-11-24 | 2017-04-26 | 江苏爱西施科技服务咨询股份有限公司 | Laying hen feed additive containing modified silkworm chrysalis amino acids and preparation method thereof |
| CN107041466A (en) * | 2016-11-24 | 2017-08-15 | 江苏爱西施科技服务咨询股份有限公司 | A kind of dorking addition agent containing modified abundant amino acids from silkworm pupa and preparation method thereof |
-
2007
- 2007-09-07 CN CNA2007101314082A patent/CN101380053A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103288946A (en) * | 2013-05-17 | 2013-09-11 | 广西大学 | Preparation method of white, low-fat and odorless silkworm pupa protein |
| CN104286846A (en) * | 2014-09-22 | 2015-01-21 | 广东省农业科学院蚕业与农产品加工研究所 | Preparation method of silkworm chrysalis food with alcohol flavor |
| CN104304639A (en) * | 2014-09-22 | 2015-01-28 | 广东省农业科学院蚕业与农产品加工研究所 | Preparation method of concentrated silkworm chrysalis protein powder without abnormal smell |
| CN104286846B (en) * | 2014-09-22 | 2016-08-24 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of preparation method of alcohol flavour silkworm chrysalis food |
| CN105341630A (en) * | 2015-12-14 | 2016-02-24 | 南通那芙尔服饰有限公司 | Preparation method for deodorized silkworm pupae |
| CN106578602A (en) * | 2016-11-24 | 2017-04-26 | 江苏爱西施科技服务咨询股份有限公司 | Laying hen feed additive containing modified silkworm chrysalis amino acids and preparation method thereof |
| CN107041466A (en) * | 2016-11-24 | 2017-08-15 | 江苏爱西施科技服务咨询股份有限公司 | A kind of dorking addition agent containing modified abundant amino acids from silkworm pupa and preparation method thereof |
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Open date: 20090311 |