CN101331833A - Use of AM phosphorus bacteria in seaside solonchak implantation - Google Patents
Use of AM phosphorus bacteria in seaside solonchak implantation Download PDFInfo
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- CN101331833A CN101331833A CNA2008100208019A CN200810020801A CN101331833A CN 101331833 A CN101331833 A CN 101331833A CN A2008100208019 A CNA2008100208019 A CN A2008100208019A CN 200810020801 A CN200810020801 A CN 200810020801A CN 101331833 A CN101331833 A CN 101331833A
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- Prior art keywords
- microbial inoculum
- phosphorus decomposing
- bacterium
- yellow sand
- solonchak
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- OAICVXFJPJFONN-UHFFFAOYSA-N phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 title claims abstract description 43
- 229910052698 phosphorus Inorganic materials 0.000 title claims description 40
- 239000011574 phosphorus Substances 0.000 title claims description 40
- 241000894006 Bacteria Species 0.000 title claims description 27
- 238000002513 implantation Methods 0.000 title 1
- 239000002689 soil Substances 0.000 claims abstract description 42
- 241001123597 Funneliformis mosseae Species 0.000 claims abstract description 6
- 239000002068 microbial inoculum Substances 0.000 claims description 30
- 239000004576 sand Substances 0.000 claims description 19
- 241000196324 Embryophyta Species 0.000 claims description 17
- 230000000050 nutritive Effects 0.000 claims description 11
- 241000694025 Sorghum x drummondii Species 0.000 claims description 10
- 235000015503 Sudangrass Nutrition 0.000 claims description 10
- 239000011159 matrix material Substances 0.000 claims description 9
- 238000005516 engineering process Methods 0.000 claims description 7
- 230000001954 sterilising Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 244000037666 field crops Species 0.000 claims description 3
- 238000007639 printing Methods 0.000 claims description 3
- 241000317178 Claroideoglomus etunicatum Species 0.000 claims description 2
- 241000235503 Glomus Species 0.000 claims description 2
- 239000003643 water by type Substances 0.000 claims description 2
- 241000250499 Rhizophagus diaphanus Species 0.000 claims 1
- 239000003337 fertilizer Substances 0.000 abstract description 8
- 239000011780 sodium chloride Substances 0.000 abstract description 7
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 4
- 230000000844 anti-bacterial Effects 0.000 abstract 2
- 239000003899 bactericide agent Substances 0.000 abstract 2
- 230000000644 propagated Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 12
- 239000002686 phosphate fertilizer Substances 0.000 description 6
- 241001232787 Epiphragma Species 0.000 description 3
- 241000219793 Trifolium Species 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 210000003608 Feces Anatomy 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000918585 Pythium aphanidermatum Species 0.000 description 2
- 235000012970 cakes Nutrition 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000000813 microbial Effects 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 240000003917 Bambusa tulda Species 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N Carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 1
- 241000289763 Dasygaster padockina Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 210000003491 Skin Anatomy 0.000 description 1
- 230000003213 activating Effects 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 229930002875 chlorophylls Natural products 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 201000009910 diseases by infectious agent Diseases 0.000 description 1
- 230000003203 everyday Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 150000002240 furans Chemical class 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002015 leaf growth Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- -1 nitrogen phosphorus Chemical compound 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 150000003016 phosphoric acids Chemical class 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 239000001038 titanium pigment Substances 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 238000009333 weeding Methods 0.000 description 1
Abstract
The invention relates to the application of AM phosphate-solubilizing bactericide in large scale ecological planting in seashore saline soil. In the application, after the AM glomus mosseae is propagated, the AM phosphate-solubilizing bactericide is made and added in a nutrition pot of the crops in the seashore saline soil, therefore the content of efficient phosphor in the soil of the root of the crops is improved remarkably. Additionally, the yield of the saline soil economic crops is increased. The application overcomes the shortcomings of the pollution brought by using the inorganic phosphor fertilizer and the low efficiency and provides an effective method for improving the crop planting in the seashore saline soil and increasing the yield.
Description
Technical field
This invention relates to the application of a kind of microorganism solution phosphobacterin, and the plantation of solonchak by the sea that relates in particular to a kind of AM phosphorus decomposing microbial inoculum is used, and belongs to field of ecological planting technology.
Background technology
From 1998 to 2007, China's arable area dropped to 18.26 hundred million mu from 19.45 hundred million mu, owing to reasons such as city-building, agricultural structure adjustment and disaster accidents, loses about 1,320 ten thousand mu of arable area every year on average.Along with the continuous growth of population, population will further increase the pressure in soil.In line with the principle of " do not strive grain with the people, do not strive ground with grain ", the soil of beach beach is utilized as us and alleviates population and provide a feasible direction to the pressure of ploughing.At present China's seaside saline-soil is nearly 0.2 hundred million hectares, and about 3,000,000 hectares of beach solonchak areas have become the important source of the important reserve land resources of China, and therefore, developing coastal beach soil is task very urgent and important in China's agricultural production.Along with salt tolerant crop is bred and the discovery of salt tolerant economic plants, the nutrition condition of these plants will become the problem that will solve from now on.Yet salt affected soil another characteristics except that the salinity height are that the soluble phosphoric acid salt content is low, and the overwhelming majority exists with the insoluble phosphate form.Promptly because the special ecotope feature of beach system, particularly seaside saline-soil high salinity, nitrogen phosphorus and the low characteristic of the content of organic matter are having a strong impact on plant growth, make that the crop seed germination rate is low, weak, the easily lodging of growth of seedling gesture finally causes declining to a great extent of output.The validity that improves phosphorus in the soil at present promotes that the technology of high-yield plant mainly is to apply phosphate fertilizer, but because the fixation in soil effect, the availability of phosphate fertilizer is lower, generally is no more than 20%.The phosphate fertilizer in China every year 1/3 needs import, and compound fertilizer and microbial manure utilization rate are less than 30% (developed country reaches 50%).And the excessive input of agricultural chemicals, chemical fertilizer etc. can cause pollution of area source to the beach system, influences the sustainable development of whole system.
Phosphorus decomposing effect that there are some researches show AM mycorhiza bacterium is very obvious, can promote plant growing.After discovery AM mycorhiza bacterium such as Su You ripple were infected clover in 2003, in order to satisfy the needs of clover P element, AM mycorhiza secretion phosphatase comes the organic P of activating soil and absorbs to be transported to clover, the organic P content of the local soil that enzymic activity is high obviously reduces, illustrate that the AM mycorrhizal fungi can strengthen the activity of soil phosphatase, can promote the hydrolysis (" phosphatase of different AM mycorhiza bacterium secretions is to the influence of rhizosphere soil organic phosphor " of the organic P of soil, this literary composition is published in soil, 2003,35 (4): 334-338).AM mycorhiza bacterium is and a class fungi of plant roots symbiosis, wants to reach the promotion plant growth, significantly improves the ideal effect of output, just must guarantee that plant rhizosphere has a considerable amount of AM mycorhiza bacterium to exist and reaches higher infection rate.Therefore, although at present existingly studies show that the AM bacterium has good phosphorus decomposing effect,, also just can't reach the effect that phosphorus decomposing is also increased production if not treatedly just can't be applied to field planting.
Summary of the invention
Technical problem to be solved by this invention provides the application among the large scale ecological plantation of solonchak by the sea of a kind of AM microbial inoculum.
The present invention is achieved through the following technical solutions for solving the problems of the technologies described above: a kind of AM phosphorus decomposing microbial inoculum is the application in the solonchak plantation by the sea, may further comprise the steps:
1) the AM bacterium is expanded numerous: with the yellow sand is culture matrix, yellow sand is sieved and carry out moist heat sterilization, and be host plant with the Sudan grass, in cultivation tray, spread yellow sand from the bottom up successively, the AM bacterium, yellow sand, AM bacterium, the Sudan grass seed, yellow sand waters and is placed on the cultivation indoor cultivation after permeable;
2) microbial inoculum is collected: after 30 days the acrial part of Sudan grass is all cut, kept intramatrical root, with the AM phosphorus decomposing microbial inoculum that promptly obtains after the suitable airing of matrix preparing;
3) combining of AM phosphorus decomposing microbial inoculum and nutritive cube technology: select the field piece to set up the seedbed, the seedbed applies AM phosphorus decomposing microbial inoculum, is mixed into nutritive cube matrix with soil, the addition of AM microbial inoculum is every mu of 12 ~ 16kg, the system alms bowl, with planting seed in nutritive cube, at last with seedling replanting in the alms bowl to the land for growing field crops.
Wherein, the AM bacterium is at least a kind of in Glomus mosseae (Glomus Mosseae) printing opacity sacculus mould (G1omus diaphanum) children cover sacculus mould (Glomus etunicatum) and four kinds of AM mycorhiza bacterium of Scotland sacculus mould (Glomus caledoniun).
As reasonable scheme of the present invention, yellow sand is crossed 20 mesh sieves, and the moist heat sterilization time is 1 hour, and the thickness of every layer of yellow sand is 1 ~ 2cm.
The present invention has following beneficial effect and advantage compared with prior art:
The validity that improves phosphorus in the soil at present promotes that the technology of high-yield plant mainly is to apply phosphate fertilizer, but because the fixation in soil effect, the availability of phosphate fertilizer is lower, generally is no more than 20%.The phosphate fertilizer in China every year 1/3 needs import, and compound fertilizer and microbial manure utilization rate are less than 30% (developed country reaches 50%).
The effect of AM phosphorus decomposing microbial inoculum is exactly with the plain compound decomposition of insoluble phosphorus or is dissolved as the titanium pigment element, for the plant absorbing utilization, improves availability of soil phosphorus, increases crop yield.This invention is applied to AM phosphorus decomposing microbial inoculum the seaside saline-soil plantation first, has improved soil available phosphorus content significantly.In addition, the output of solonchak economic crops also increases.The present invention has overcome and has used pollution and the low defective of utilization ratio that inorganic phosphorus fertilizer brings in the past, is improvement seaside saline-soil crop-planting, and improving output provides effective method.
Embodiment
AM mycorhiza bacterium expanded make AM phosphorus decomposing microbial inoculum after numerous and add in the nutritive cube of seaside saline-soil crop, thereby improve the content of available phosphorus in the crop root soil, embodiment is as follows:
1) the AM bacterium is expanded numerous: with the yellow sand is culture matrix, yellow sand is crossed 20 mesh sieves and carry out moist heat sterilization 1h under 121 ℃ of conditions, and with the Sudan grass host plant, in cultivation tray, spread yellow sand, AM bacterium, yellow sand from the bottom up successively, the AM bacterium, the Sudan grass seed, yellow sand, every layer thickness 1 ~ 2cm, water to be placed on after permeable and cultivate indoor cultivation and got final product in 30 days, water every day once, guarantee the moisture requirement of Sudan grass, vigorous growth.
2) microbial inoculum is collected: after 30 days the acrial part of Sudan grass is all cut, kept intramatrical root, with the AM microbial inoculum that promptly obtains after the suitable airing of matrix preparing, put into valve bag and preserve stand-by.
3), combining of AM phosphorus decomposing microbial inoculum and nutritive cube technology:
A) make the seedbed.Select fertile, the well-drained field of soil property piece, set up the seedbed of 1.3m X 7.5m specification near growing area; Cake fertilizer and AM phosphorus decomposing microbial inoculum are executed in the seedbed, are mixed into nutritive cube matrix with soil.The addition of final assurance cake fertilizer is every mu of 3-4 jin, and the addition of AM microbial inoculum is every mu of 12-16kg; With the machine for making pots system alms bowl of bore 8cm, dark 12cm, totally 2000 nutritive cubes.
Be set at and add AM phosphorus decomposing microbial inoculum and two kinds of processing of contrast.
B), sowing and overlay film: seed is placed on vernalization under the proper temperature, treats that most of seed kind skin begins to split when showing money or valuables one carries unintentionally it to be seeded in the nutritive cube that every alms bowl grain number is decided on situations such as seed sizes.Water sufficient moisture, with in 10 minutes no longer seepage be standard.The lid layer fine earth is advisable with 1.5-2.0cm then.0.2kg furans pellet is evenly spread fertilizer over the fields on the seedbed control cutworm.Spray 300-400 times of carbendazim soup again, control plant damping off and damping off.Spray 200-250 times of weeding then and prevent and treat the seedbed weeds only.The epiphragma that on the seedbed, tiles, with being embedded in around the film in the soil sealing tight, improve warming effect.Hold drain ditch all around, seedbed simultaneously successfully, prevent seedbed ponding.After emerging, with bamboo frame bow film, be barricaded as plastic canopy, height is advisable with 20-30cm.To 2 true leaf stages temperature is controlled at 25 ℃-30 ℃ to seedling is long from emerging, helps root of hair and promote leaf growth.When environmental temperature is higher than 18 ℃, take off full film daytime, night, epiphragma kept two to ventilate simultaneously.Transplant last week by day and night taking off the film hardening, make growth of seedling adapt to atmospheric temperature gradually, epiphragma when raining keeps two to ventilate.
C), transplant seedlings: carry out and broadcast the back management, last week want hardening, make growth of seedling adapt to atmospheric temperature gradually,, carry out the transition to and day take off night dew by taking off the lid at night day in transplanting.At last with seedling replanting to the land for growing field crops, measure in its process of growth that viable count, seedling percent, plant height, stem are thick, fresh weight, dry weight, available phosphorus content, output and chlorophyll content, can find that the plant growth of using AM phosphorus decomposing microbial inoculum obviously is better than control group.
When the AM bacterium was Glomus mosseae, soil available phosphorus was brought up to 38.32mg/Kg from the 32.34mg/Kg of control soil; When AM mycorhiza bacterium is that the printing opacity sacculus is when mould; Soil available phosphorus is brought up to 37.99mg/Kg from the 32.34mg/Kg of control soil; When the AM bacterium is children cover sacculus when mould: soil available phosphorus is brought up to 37.23mg/Kg from the 32.34mg/Kg of control soil; When the AM bacterium is that Scotland sacculus is when mould; Soil available phosphorus is brought up to 36.89mg/Kg from the 32.34mg/Kg of control soil; When the AM bacterium be above-mentioned four kinds of mycorhiza bacterium with 1: 1: 1: 1 when mixing; Soil available phosphorus is brought up to 39.53mg/Kg from the 32.34mg/Kg of control soil, and the phosphorus decomposing effect of visible AM phosphorus decomposing microbial inoculum is remarkable.
Claims (5)
1, a kind of AM phosphorus decomposing microbial inoculum application in the solonchak plantation by the sea is characterized in that may further comprise the steps:
1) the AM bacterium is expanded numerous: with the yellow sand is culture matrix, yellow sand is sieved and carry out moist heat sterilization, and be host plant with the Sudan grass, in cultivation tray, spread yellow sand from the bottom up successively, the AM bacterium, yellow sand, AM bacterium, the Sudan grass seed, yellow sand waters and is placed on the cultivation indoor cultivation after permeable;
2) microbial inoculum is collected: after 30 days the acrial part of Sudan grass is all cut, kept intramatrical root, with the AM phosphorus decomposing microbial inoculum that promptly obtains after the suitable airing of matrix preparing;
3) combining of AM phosphorus decomposing microbial inoculum and nutritive cube technology: select the field piece to set up the seedbed, the seedbed applies AM phosphorus decomposing microbial inoculum, is mixed into nutritive cube matrix with soil, the addition of AM microbial inoculum is every mu of 12～16kg, the system alms bowl, with planting seed in nutritive cube, at last with seedling replanting in the alms bowl to the land for growing field crops.
2, AM phosphorus decomposing microbial inoculum according to claim 1 application in the solonchak plantation by the sea is characterized in that: described AM bacterium is at least a kind of in Glomus mosseae (Glomus Mosseae) printing opacity sacculus mould (Glomus diaphanum) children cover sacculus mould (Glomus etunicatum) and four kinds of AM mycorhiza bacterium of Scotland sacculus mould (Glomus caledoniun).
3, AM phosphorus decomposing microbial inoculum according to claim 1 application in the solonchak plantation by the sea, it is characterized in that: described yellow sand is crossed 20 mesh sieves.
4, AM phosphorus decomposing microbial inoculum according to claim 1 application in the solonchak plantation by the sea, it is characterized in that: the described moist heat sterilization time is 1 hour.
5, AM phosphorus decomposing microbial inoculum according to claim 1 application in the solonchak plantation by the sea, it is characterized in that: the thickness that described yellow sand is every layer is 1～2cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100208019A CN101331833A (en) | 2008-07-25 | 2008-07-25 | Use of AM phosphorus bacteria in seaside solonchak implantation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100208019A CN101331833A (en) | 2008-07-25 | 2008-07-25 | Use of AM phosphorus bacteria in seaside solonchak implantation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101331833A true CN101331833A (en) | 2008-12-31 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008100208019A Pending CN101331833A (en) | 2008-07-25 | 2008-07-25 | Use of AM phosphorus bacteria in seaside solonchak implantation |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102191199A (en) * | 2011-03-25 | 2011-09-21 | 合肥工业大学 | High-efficiency composite dephosphorizing bacterial agent and preparation method thereof |
| CN102603372A (en) * | 2012-04-06 | 2012-07-25 | 中国烟草总公司山东省公司 | Simple production technology of special AM (arbuscular mycorrhiza) fungal manure for tobacco |
| CN103460946A (en) * | 2013-09-18 | 2013-12-25 | 南京大学连云港高新技术研究院 | Method for improving survival rate of kosteletzkya virginica planted in seaside saline soil |
| CN112292952A (en) * | 2020-09-28 | 2021-02-02 | 中国科学院南京土壤研究所 | Method for rapidly improving nutrient storage capacity and soil fertility of saline-alkali farmland soil in irrigation area |
| CN112425476A (en) * | 2020-11-10 | 2021-03-02 | 深圳市华美绿生态环境集团有限公司 | Mangrove plant seedling raising substrate and seedling raising method |
-
2008
- 2008-07-25 CN CNA2008100208019A patent/CN101331833A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102191199A (en) * | 2011-03-25 | 2011-09-21 | 合肥工业大学 | High-efficiency composite dephosphorizing bacterial agent and preparation method thereof |
| CN102603372A (en) * | 2012-04-06 | 2012-07-25 | 中国烟草总公司山东省公司 | Simple production technology of special AM (arbuscular mycorrhiza) fungal manure for tobacco |
| CN102603372B (en) * | 2012-04-06 | 2013-12-25 | 中国烟草总公司山东省公司 | Simple production technology of special AM (arbuscular mycorrhiza) fungal manure for tobacco |
| CN103460946A (en) * | 2013-09-18 | 2013-12-25 | 南京大学连云港高新技术研究院 | Method for improving survival rate of kosteletzkya virginica planted in seaside saline soil |
| CN112292952A (en) * | 2020-09-28 | 2021-02-02 | 中国科学院南京土壤研究所 | Method for rapidly improving nutrient storage capacity and soil fertility of saline-alkali farmland soil in irrigation area |
| CN112292952B (en) * | 2020-09-28 | 2022-03-11 | 中国科学院南京土壤研究所 | Method for rapidly improving nutrient storage capacity and soil fertility of saline-alkali farmland soil in irrigation area |
| CN112425476A (en) * | 2020-11-10 | 2021-03-02 | 深圳市华美绿生态环境集团有限公司 | Mangrove plant seedling raising substrate and seedling raising method |
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Open date: 20081231 |