CN101240267A - SOD complex enzyme and preparation method thereof - Google Patents
SOD complex enzyme and preparation method thereof Download PDFInfo
- Publication number
- CN101240267A CN101240267A CNA2008100849893A CN200810084989A CN101240267A CN 101240267 A CN101240267 A CN 101240267A CN A2008100849893 A CNA2008100849893 A CN A2008100849893A CN 200810084989 A CN200810084989 A CN 200810084989A CN 101240267 A CN101240267 A CN 101240267A
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- CN
- China
- Prior art keywords
- sod
- ethanol
- propolis
- prozyme
- stir
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 102000004190 Enzymes Human genes 0.000 title abstract description 5
- 108090000790 Enzymes Proteins 0.000 title abstract description 5
- 241000241413 Propolis Species 0.000 claims abstract description 15
- 235000013336 milk Nutrition 0.000 claims abstract description 15
- 239000008267 milk Substances 0.000 claims abstract description 15
- 210000004080 milk Anatomy 0.000 claims abstract description 15
- 229940069949 propolis Drugs 0.000 claims abstract description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 46
- 238000003756 stirring Methods 0.000 claims description 25
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 24
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 15
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 12
- 229930006000 Sucrose Natural products 0.000 claims description 12
- 239000012153 distilled water Substances 0.000 claims description 12
- 239000004310 lactic acid Substances 0.000 claims description 12
- 235000014655 lactic acid Nutrition 0.000 claims description 12
- 239000005720 sucrose Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000000706 filtrate Substances 0.000 claims description 10
- 239000002244 precipitate Substances 0.000 claims description 10
- 230000003068 static effect Effects 0.000 claims description 10
- 239000006228 supernatant Substances 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 6
- 238000001556 precipitation Methods 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 239000000284 extract Substances 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 10
- 210000004369 blood Anatomy 0.000 abstract description 4
- 239000008280 blood Substances 0.000 abstract description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- 231100000252 nontoxic Toxicity 0.000 abstract description 3
- 230000003000 nontoxic effect Effects 0.000 abstract description 3
- 235000012000 cholesterol Nutrition 0.000 abstract description 2
- 230000036039 immunity Effects 0.000 abstract description 2
- 210000004204 blood vessel Anatomy 0.000 abstract 2
- 102000009027 Albumins Human genes 0.000 abstract 1
- 108010088751 Albumins Proteins 0.000 abstract 1
- 230000003213 activating effect Effects 0.000 abstract 1
- 230000032683 aging Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 206010013786 Dry skin Diseases 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 230000008014 freezing Effects 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 150000002576 ketones Chemical class 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 206010047073 Vascular fragility Diseases 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 229960004676 antithrombotic agent Drugs 0.000 description 1
- 238000006701 autoxidation reaction Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- -1 oxygen free radical Chemical class 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229940079877 pyrogallol Drugs 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000024883 vasodilation Effects 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a health product, in particular to a SOD complex enzyme and a preparation method thereof. In the process of preparing the SOD complex enzyme, the albumin type SOD extracted from the fresh milk has high enzyme activity and has various effects of resisting oxidation, delaying human body aging and the like. The propolis in the formula has the effects of reducing blood fat and cholesterol, enhancing the blood vessel expansion force, reducing the blood vessel fragility, activating cells, improving the immunity and the like. The functional effect of the two is greatly improved after the two are scientifically combined, and the medicine is safe, non-toxic and pollution-free.
Description
Technical field
The present invention relates to a kind of healthcare products, particularly relate to a kind of SOD prozyme and preparation method thereof.
Background technology
SOD (dismutase) is a single-minded scavenging agent of removing excess oxygen free radical in the human body, and universally acknowledged antioxidant is a delaying human body caducity, improves the important substance of body immunity.Use animal blood (pig, ox, sheep) to extract so far both at home and abroad always.In blood plasma, at first add antithrombotics, in thermal change also to add chemical feedstockss such as acetone, chlorinated ketone thereafter, these chemical feedstockss have toxicity, residual in the product has suitable harm to human body, need just can be used for oral through specific purification, its purification cost is very high, and the use of country's restriction acetone and chlorinated ketone, the freshness of its raw material blood requires also very high, and scale operation SOD is restricted.
Summary of the invention
Purpose of the present invention is exactly the deficiency that overcomes existing SOD extractive technique, provide a kind of albumosease type SOD that from fresh milk, extracts and with the propolis compatibility, form the SOD prozyme.It has not only solved the complicacy of producing, and safety non-toxic, and the functional effect of its SOD prozyme product will be higher than the SOD that sells in the market far away, is fit to scale operation and popularization.
An object of the present invention is to provide a kind of SOD prozyme.
Another object of the present invention provides the preparation method of described SOD prozyme.
The objective of the invention is to be achieved through the following technical solutions:
A kind of SOD prozyme is formed by the feedstock production of following parts by weight;
Fresh milk 25-40 distilled water 80-100
Ethanol 25-40 lactic acid 0.4-1.0
Propolis 0.5-0.8 sucrose 0.3-0.6
Its concentration of ethanol of the present invention is 95%.
Preferably, a kind of SOD prozyme is formed by the feedstock production of following parts by weight;
Fresh milk 30 distilled water 90
Ethanol 30 lactic acid 0.6
Propolis 0.65 sucrose 0.5
The preparation method of a kind of SOD prozyme of the present invention may further comprise the steps;
(1) take by weighing each raw material by formula ratio, earlier lactic acid is joined in the distilled water, stir, add 1/3 of fresh milk and ethanol total amount more simultaneously, stir, precipitate 7-8 hour, filter, collect filtrate, standby;
(2) 3/10 of sucrose and ethanol total amount joined respectively in the filtrate of step 1, stir, the sodium hydroxide solution that adds 40 mol is again regulated pH value to 7.4, precipitates static 10-15 hour, filters the collecting precipitation thing, and cryodrying is ground into fine powder, and is standby;
(3) propolis is dissolved in the remaining ethanol, stirs, place after 10-12 hour, extract supernatant liquor, with the static 70-75 of supernatant liquor hour, after the superfreeze, be ground into fine powder, the fine powder with step 2 stirs again, promptly finishes.
Cryodrying temperature described in the preparation method of the present invention is at subzero 4-5 ℃, and the superfreeze temperature is at subzero 40-50 ℃.
In a kind of SOD prozyme of the present invention refabrication process, the albumosease type SOD that it extracts from fresh milk, its enzyme activity height, it has anti-oxidant, many effects such as delaying human body caducity.Propolis in the prescription has blood fat reducing and cholesterol and strengthens vasodilation power, reduces vascular fragility, and active cells improves effects such as immunizing power.The two its functional effect behind the science compatibility improves greatly, and safety non-toxic is nuisanceless.
Embodiment
Following examples are in order further to describe the present invention for example, rather than limit the present invention by any way.
Embodiment 1 (unit: kilogram)
Fresh milk 30 distilled water 90
Ethanol 30 lactic acid 0.8
Propolis 0.65 sucrose 0.5
Its preparation method is as follows:
(1) take by weighing each raw material by formula ratio, earlier lactic acid is joined in the distilled water, stir, add fresh milk and 10kg ethanol more simultaneously, stir, precipitate 7 hours, filter, collect filtrate, standby;
(2) sucrose and 9kg ethanol are joined respectively in the filtrate of step 1, stir, the sodium hydroxide solution that adds 40 mol is again regulated pH value to 7.4, precipitates static 12 hours, filters the collecting precipitation thing, and the subzero 4 ℃ of dryings of low temperature are ground into fine powder, and are standby;
(3) propolis is dissolved in the remaining ethanol, stirs, places after 12 hours, the extraction supernatant liquor, with static 72 hours of supernatant liquor, very low temperature subzero 45 ℃ freezing after, be ground into fine powder, the fine powder with step 2 stirs again, promptly finishes.
Embodiment 2 (unit: kilogram)
Fresh milk 38 distilled water 95
Ethanol 36 lactic acid 0.6
Propolis 0.75 sucrose 0.5
Its preparation method is as follows:
(1) take by weighing each raw material by formula ratio, earlier lactic acid is joined in the distilled water, stir, add fresh milk and 12kg ethanol more simultaneously, stir, precipitate 8 hours, filter, collect filtrate, standby;
(2) sucrose and 10.8kg ethanol are joined respectively in the filtrate of step 1, stir, the sodium hydroxide solution that adds 40 mol is again regulated pH value to 7.4, precipitates static 13 hours, filters the collecting precipitation thing, and the subzero 5 ℃ of dryings of low temperature are ground into fine powder, and are standby;
(3) propolis is dissolved in the remaining ethanol, stirs, places after 11 hours, the extraction supernatant liquor with static 73 hours of supernatant liquor, after ultralow subzero 40 ℃ of temperature are freezing, is ground into fine powder, and the fine powder with step 2 stirs again, promptly finishes.
Embodiment 3 (unit: kilogram)
Fresh milk 26 distilled water 80
Ethanol 27 lactic acid 0.5
Propolis 0.55 sucrose 0.4
Its preparation method is as follows:
(1) take by weighing each raw material by formula ratio, earlier lactic acid is joined in the distilled water, stir, add fresh milk and 9kg ethanol more simultaneously, stir, precipitate 8 hours, filter, collect filtrate, standby;
(2) sucrose and 8.1kg ethanol are joined respectively in the filtrate of step 1, stir, the sodium hydroxide solution that adds 40 mol is again regulated pH value to 7.4, precipitates static 12 hours, filters the collecting precipitation thing, and the subzero 4 ℃ of dryings of low temperature are ground into fine powder, and are standby;
(3) propolis is dissolved in the remaining ethanol, stirs, places after 12 hours, the extraction supernatant liquor, with static 74 hours of supernatant liquor, very low temperature subzero 50 ℃ freezing after, be ground into fine powder, the fine powder with step 2 stirs again, promptly finishes.
Below by detect the proof embodiment of the invention 1 effect;
SOD prozyme examining report
Censorship people: Xu Peng lifts
Name of product: the SOD prozyme of the embodiment of the invention 1
Detect unit: life science system of Hebei Science ﹠ Technology Normal College
Testing staff: the Liu Yan of Cai Ai army virtue
Detection method: pyrogallol autoxidation method
Detected result:
The U/g of unit
-1
| Sample | The result |
| 1g | 4443 |
Claims (3)
1. SOD prozyme is formed by the feedstock production of following parts by weight;
Fresh milk 25-40 distilled water 80-100
Ethanol 25-40 lactic acid 0.4-1.0
Propolis 0.5-0.8 sucrose 0.3-0.6.
2. a kind of SOD prozyme according to claim 1 is formed by the feedstock production of following parts by weight:
Fresh milk 30 distilled water 90
Ethanol 30 lactic acid 0.6
Propolis 0.65 sucrose 0.5.
3. the preparation method of a kind of SOD prozyme according to claim 1, it may further comprise the steps:
1, take by weighing each raw material by formula ratio, earlier lactic acid is joined in the distilled water, stir, add 1/3 of fresh milk and ethanol total amount more simultaneously, stir, precipitate 7-8 hour, filter, collect filtrate, standby;
2,3/10 of sucrose and ethanol total amount joined respectively in the filtrate of step 1, stir, the sodium hydroxide solution that adds 40 mol is again regulated pH value to 7.4, precipitates static 10-15 hour, filters the collecting precipitation thing, and cryodrying is ground into fine powder, and is standby;
3, propolis is dissolved in the remaining ethanol, stirs, place after 10-12 hour, extract supernatant liquor, with the static 70-75 of supernatant liquor hour, after the superfreeze, be ground into fine powder, the fine powder with step 2 stirs again, promptly finishes.
4, the preparation method of a kind of SOD prozyme according to claim 3, wherein said cryodrying temperature are at subzero 4-5 ℃, and the superfreeze temperature is at subzero 40-50 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100849893A CN101240267B (en) | 2008-03-12 | 2008-03-12 | SOD complex enzyme and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100849893A CN101240267B (en) | 2008-03-12 | 2008-03-12 | SOD complex enzyme and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101240267A true CN101240267A (en) | 2008-08-13 |
| CN101240267B CN101240267B (en) | 2011-07-27 |
Family
ID=39932098
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008100849893A Expired - Fee Related CN101240267B (en) | 2008-03-12 | 2008-03-12 | SOD complex enzyme and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101240267B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103361320A (en) * | 2013-07-25 | 2013-10-23 | 天津核生科技生物工程有限公司 | Method of extracting SOD from milk |
-
2008
- 2008-03-12 CN CN2008100849893A patent/CN101240267B/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103361320A (en) * | 2013-07-25 | 2013-10-23 | 天津核生科技生物工程有限公司 | Method of extracting SOD from milk |
| CN103361320B (en) * | 2013-07-25 | 2014-06-18 | 天津核生科技生物工程有限公司 | Method of extracting SOD from milk |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101240267B (en) | 2011-07-27 |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110727 Termination date: 20140312 |