CN101216490A - Dry chemical method TORCH detection reagent kit and method of manufacture - Google Patents
Dry chemical method TORCH detection reagent kit and method of manufacture Download PDFInfo
- Publication number
- CN101216490A CN101216490A CNA2008100194815A CN200810019481A CN101216490A CN 101216490 A CN101216490 A CN 101216490A CN A2008100194815 A CNA2008100194815 A CN A2008100194815A CN 200810019481 A CN200810019481 A CN 200810019481A CN 101216490 A CN101216490 A CN 101216490A
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- antigen
- damping fluid
- sodium azide
- torch
- kit
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Abstract
The invention relates to a TORCH detection kit using a dry chemistry method and a preparation method of the kit. The detection kit comprises a detection unit consisting of a PVC strip for supporting a reaction system, a cellulose nitrate membrane reaction solution system for adsorbing reactants, a protein stabilizer and sodium azide; an enhancement solution containing sodium chloride and sodium azide; a binding solution containing alkaline phosphatase dissolved in a buffer solution and combined with goat anti human body, a protein stabilizer and sodium azide; and a developing solution containing a developing substrate dissolved in a buffer solution. The entire kit also includes a disposable tube for allowing the test strip to react therein and an anti-moisture drying agent. The kit of the invention has high detection speed, low detection cost, and no need for any auxiliary equipment as well as a great amount of expensive detection equipment in the detection process. The kit helps to develop rapid TORCH screening in various occasions in clinic units and family planning systems of various levels, thus promoting national health.
Description
Technical field
The present invention relates to whether contain in a kind of fast detecting human serum/blood plasma/whole blood the manufacture method of kit and this kit of TORCH antiviral antibody.
Background technology
TORCH is meant: the abbreviation of the English name of arc worm, rubella virus, cytomegalovirus, these four kinds of pathogen of herpes simplex virus, this is an adopted name as can be seen from the article name of list of references, they are children's virus infectionses, the main pathogenic microbes of virus infections in the pregnancy period.TORCH detects extremely important meaning, when being used to judge children's clinical infection type, can know clinical in different virus infections degree selection therapeutic regimens; Infect when judging when being used for the pregnant woman, can reduce the occurrence rate of inborn defect.
The method that is used for the detection of TORCH now has: immunofluorescence technique, complement combined techniques, passive hemagglutination, neutralization reaction, ELISA, gold mark percolation, gold mark chromatography.The sample that detects all uses serum, can also need to use fluorescent microscope, microplate reader etc.The common drawback of these detection methods is all to require the necessary instrument of specialty or need the technical professional just can operate.
Summary of the invention
The objective of the invention is to overcome the deficiency that exists in the existing detection technique, provide a kind of simple, convenient, do not need to rely on professional instrument and professional person just whether to contain the dry chemical method TORCH detection kit of TORCH antiviral antibody in energy qualitative detection human serum/blood plasma/whole blood.
Another object of the present invention then provides a kind of preparation method of mentioned reagent box.
According to technical scheme provided by the invention: in the detecting unit of detection kit, comprise the matrix lath that is used to support reaction system;
The film material that is used for the adsorption reaction thing, this film material is fitted on the matrix lath;
Be attracted to the antigen on the film material, be to comprise arc worm, giant cell, rubella virus, four kinds of antigens of herpes simplex, in a certain order, some film device with accurate pipettor or robotization, with the concentration of 1.0～3.0mg/ml, the amount bag of 1～3ul is by in the fenestra to the matrix lath.Described film material can be a nitrocellulose filter, perhaps the film material that PVDF membrane PVDF etc. can adsorbed proteins.
Reactant in the kit comprises dilution, strengthens liquid, in conjunction with liquid and development liquid; Reactant is used for specifically, and whether the detected sample of qualitative detection contains TORCH antibody.Wherein said dilution comprises: 1～5% protein stabiliser and 0.01～0.1% Sodium azide, and surplus is the PB damping fluid, wherein the pH of damping fluid is 6.0～8.2; Described enhancing liquid includes 0.9～5.7% sodium chloride and 0.01～0.1% Sodium azide, Yu Weishui, and unit is a mass percent.
Described alkaline phosphatase, 1～5% protein stabiliser and 0.01～0.1% Sodium azide in conjunction with liquid bag and the anti-people's antibodies of animal, surplus is damping fluid, the unit of protein stabiliser, Sodium azide and damping fluid is a mass percent; Described and the alkaline phosphatase anti-people's antibodies of animal is dissolved in the damping fluid, and the pH of damping fluid is 6.0～8.8, and the final concentration of alkaline phosphatase is 1～5mg/ml.
Described development liquid comprises that concentration is the chromogenic substrate of 0.5～3mg/ml, 0.01～0.1% Sodium azide, and surplus is damping fluid, wherein the unit of Sodium azide and damping fluid is a mass percent; It is in 8.8～11.0 the damping fluid that chromogenic substrate is dissolved in pH; Described chromogenic substrate is 5-bromo-4-chloro-3-indyl phosphate, a kind of among nitro tetrazolium chloride blue NBT, tetramethyl benzidine TMB, the o-phenylenediamine OPD.
In kit, also comprise and be used for the disposable pipe that reacts for reagent strip and prevent moist drying agent.
The manufacture method of described dry chemical method TORCH detection kit comprises following steps:
(1) preparation of reactant
A, preparation TORCH antigen; Described TORCH antigen comprises: toxoplasma antigen, rubella virus antigen, cytomegalovirus antigen, herpes simplex virus antigens;
B, the preparation anti-people's antibody of animal and alkaline phosphatase;
The anti-people's antibody of described animal comprises goat anti-human antibody, the anti-people's antibody of rabbit, the anti-people's antibody of horse or the anti-people's antibody of donkey; These anti-people's antibody all can outsourcing;
C, preparation are used to support the lath of reaction system;
With of the dimensioned moulding of matrix lath, on the matrix lath, beat several reacting holes then according to reagent strip; The film material that will be used for the adsorption reaction thing again sticks on the back side of matrix lath, to cover the reacting hole on the lath;
(2) preparation of kit
The bag quilt and the processing of a, reactant
(a), the bag of TORCH antigen quilt and drying:
With ready TORCH antigen: toxoplasma antigen, giant cell antigen, rubella virus antigen, herpes simplex virus antigens are successively with pipettor or some film device, concentration with 1.0～3.0mg/ml, by in the reacting hole to the matrix lath, shady and cool place is dry with the amount bag of 1～3ul;
(b), bag is handled by the sealing of good reaction strip;
The matrix lath that bag is adsorbed soaked 0.5～1 hour with the lock solution of 5～20g/L, and the buffer solution for cleaning rear venting that takes out with 0.1M dries; Described lock solution comprises: the bovine serum albumin(BSA) of 1～5% mass concentration (BSA), 1.5～5% sucrose, 0.01～0.1% Sodium azide;
(c), the preparation of reactant
According to following proportioning preparation reactant: this reactant comprises dilution, strengthens liquid, in conjunction with liquid and development liquid; Following unit is except that indicating especially, and all the other are mass percent;
Wherein, the consisting of of dilution: 1～5% protein stabiliser and 0.01～0.1% Sodium azide, surplus is damping fluid, the pH of described damping fluid is 6.0～8.2;
Strengthen liquid: contain 0.9～5.7% sodium chloride and 0.01～0.1% Sodium azide, Yu Weishui;
In conjunction with liquid: will be that 1～5% protein stabiliser and mass concentration are that to be dissolved in pH be in 6.0～8.8 the damping fluid for 0.01～0.1% Sodium azide with alkaline phosphatase, the mass concentration of the anti-people's antibodies of animal; Wherein, the mass concentration of protein stabiliser is 1～5%, and the mass concentration of Sodium azide is 0.01～0.1%, and surplus is damping fluid, and the final concentration of alkaline phosphatase is 1～5mg/ml;
Development liquid: the chromogenic substrate and the Sodium azide that will be dissolved in pH and be in 8.8～11.0 the damping fluid mix, and wherein, the concentration of chromogenic substrate is 0.5～3mg/ml, and the mass concentration of Sodium azide is 0.01～0.1%, and surplus is damping fluid; Described chromogenic substrate comprises 5-bromo-4-chloro-3-indyl phosphate, to nitro tetrazolium chloride orchid (NBT) or tetramethyl benzidine (TMB), a kind of in the o-phenylenediamine (OPD);
The assembling of b, reagent strip and packing
(1) will seal dry matrix lath with hand papercutter and cut into reagent strip as required; Top cutting is a plate from big plate is cut into, handled easily.Be that size according to final reagent strip cuts into strip herein.
(2) reagent strip of well cutting is packed into plastic housing, the sealing moulding;
(3) reagent that will seal moulding carries out packing according to packing specification.
Described toxoplasma antigen is T.gondii, the RH strain; Described rubella virus antigen is the HPV-77 strain; Described cytomegalovirus antigen is the AD-169 strain; Described herpes simplex virus antigens is the Maclntyre strain.
Described damping fluid is PB (phosphate buffer) or PBS (phosphate buffer), also can be acetate buffer solution or other classics, buffer system commonly used.
The present invention utilizes the principle of the specific immune response of antigen-antibody in the immunology, can specificity be fixed on the immobilon-p in conjunction with the antigen of antiviral antibody, to measure sample joins in the reaction tube, insert reagent strip, different virus antibody is fixed on the film in the sample, add enzyme and chromogenic substrate again, if the position that captures antibody can show that thereby blue purple dot is pointed out in the tested sample contains target antibody, and then the proof detected person infection of once being correlated virus.
Without any need for utility appliance, can single part operate in the whole testing process, be fit to any testing agency and carry out.
Description of drawings
Fig. 1 is a kit synoptic diagram of the present invention.
Embodiment
A kind of dry chemical method TORCH quick detection kit of the present invention, (comprise dilution: the Sodium azide by damping fluid (pH6.0～8.2), protein stabiliser and 0.1% is formed to comprise the matrix lath that is used to support reaction system, the nitrocellulose filter (the film material that perhaps is used for adsorbed proteins as PVDF etc.) that is used for the adsorption reaction thing, reactant in the detecting unit of kit; Strengthen liquid: the Sodium azide that contains sodium chloride and 0.1%; In conjunction with liquid: form by the Sodium azide that is dissolved in the alkaline phosphatase that combines with goat anti-human antibody's (perhaps anti-people's antibody of other animals) in the damping fluid (pH6.0～8.8), protein stabiliser and 0.1%; Development liquid: by being dissolved in chromogenic substrate in the damping fluid (pH8.8～11.0) (can be 5-bromo-4-chloro-3-indyl phosphate, to blue NBT of nitro tetrazolium chloride or TMB, OPD etc. form with 0.1% Sodium azide).In whole kit, also comprise and be used for the disposable pipe that reacts for reagent strip and prevent moist drying agent.
Below in conjunction with specific embodiment the present invention is further described.
(1) reactant preparation
1, the purchase of TORCH antigen and preparation; Generally according to the Strain of clinical practice appearance, the TORCH antigen of outsourcing selects for use principle as follows:
Toxoplasma antigen is generally T.gondii, the RH strain;
Rubella virus antigen is generally the HPV-77 strain;
Cytomegalovirus antigen is generally the AD-169 strain;
Herpes simplex virus antigens is generally the Maclntyre strain;
Above antigen can be bought from relevant company, as BIOSOURCE, and R﹠amp; D, biodesign, AALTO or the like.
2, the purchase and the preparation of goat-anti people (the perhaps anti-people of other animals) antibody-alkaline phosphatase;
(the perhaps anti-people of other animals is as the anti-people's antibody of rabbit or anti-people's antibody of horse or the anti-people's antibody of donkey for the goat-anti people; These anti-people's antibody all are can outsourcing) antibody because of its ubiquity from domestic or foreign associated companies buy.
3, the preparation of reaction system back up pad.
Whole reaction system is to be supported by the matrix lath of a fritter hard.We according to the reacting hole position punching of design in advance, form compact arranged 6 holes then with the dimensioned moulding according to reagent strip of the matrix lath of big plate.The requirement pass is smooth, and rule does not have burr.
Nitrocellulose filter is sticked on the back side of lath according to the size of whole lath, and size is suitable with the hole that can close on the matrix lath.Compress, offset.
(2) preparation of kit, as shown in Figure 1:
1, the bag of reactant quilt and processing
(1) bag of TORCH antigen quilt and drying:
With the TORCH antigen (comprising arc worm, giant cell, rubella virus, herpes simplex virus) of outsourcing in a certain order, some film device with accurate pipettor or robotization, concentration range with 1.0～3.0mg/ml, by in the fenestra to the matrix lath, shady and cool place is dry with the amount bag of 1～3ul.
(2) bag is handled by the sealing of good reaction strip;
The matrix lath that bag is adsorbed is with the milk power solution (bovine serum albumin(BSA) that can also comprise 5% mass concentration of 5～20g/L, sucrose about 5%, Sodium azide about 0.01% (antiseptic)) soaked 0.5～1 hour, the PBS buffer solution for cleaning rear venting that takes out with 0.1M dries.
(3) preparation of reactant liquor.
Prepare correlated response liquid according to following proportioning:
Dilution: form by damping fluid (pH6.0～8.2), protein stabiliser and Sodium azide;
Strengthen liquid: contain 0.9%～5.7% sodium chloride and Sodium azide;
In conjunction with liquid: by be dissolved in the damping fluid (pH6.0～8.8) (the perhaps anti-people's antibody of other animals is as the anti-people's antibody of rabbit or anti-people's antibody of horse or the anti-people's antibody of donkey with the goat anti-human antibody; These anti-people's antibody all are can outsourcing) alkaline phosphatase, protein stabiliser and the Sodium azide of combination form;
Development liquid: by be dissolved in chromogenic substrate in the damping fluid (pH8.8～11.0) (can be 5-bromo-4-chloro-3-indyl phosphate, to the blue NBT of nitro tetrazolium chloride or TMB, OPD etc. and Sodium azide.
2, the assembling of reagent strip and packing
(1) will seal dry matrix lath with hand papercutter and cut into reagent strip as required.
(2) reagent strip of well cutting is packed into plastic housing, the sealing moulding.
(3) reagent that will seal moulding carries out packing according to packing specification.
The kit made from method of the present invention, because of various materials all in advance by immobilization, so under rated condition, can preserve 18 months, be fit to extensive the recommendation and use.
Operating process is as follows:
One, preliminary step
1, heat block is put into water bath, thermometer is taken out from box, take off the plastic sheath of mercury end, the mercury end inserts the thermometer hole of heat block downwards.The bath cabinet switch of fetching boiling water is adjusted the temperature control knob and is made the indication of thermometer be stabilized in 55 ℃ ± 1, and this moment, the temperature of reaction tube was 46 ℃ ± 2;
2, every part of test need be inserted respectively in the hole of heat block by 4 reaction tubes of taking-up from kit;
3, the water in the Washing cup will be filled it up with to scale mark, and the water surface wants to surpass all fenestras on the test-strips;
4, in first reaction tube, add 2ml dilution (#1); In second reaction tube, add 2ml and strengthen liquid (#2); In the 3rd reaction tube, add 2ml in conjunction with liquid (#3); In the 4th reaction tube, add 2ml colour developing liquid (#4);
5, before testing, wait for ten minutes so that in the pipe reagent obtain preheating, at this moment can give the test-strips label, the fenestra of attention on can not the touching test bar prepared sample simultaneously;
6, add sample to be measured (serum 10～40 μ l/20～80ul whole blood sample) to dilution reaction tube #1.
Two, test procedure
1, test-strips was prewetted in Washing cup for 30～60 seconds;
2. test-strips is inserted among the dilution reaction tube #1, moved up and down fast 20 times, so that mix reagent and sample were placed 5～60 minutes;
3, take out test-strips and put into Washing cup.For the fenestra on the test-strips is fully cleaned, need back and forth rapid movement 10～15 seconds;
4, test-strips is inserted among the enhancing liquid reaction tube #2, moved up and down fast 20 times, mix reagent and sample were placed 5～8 minutes;
5, take out test-strips and in Washing cup, clean (with the 3rd step);
6, test-strips is inserted in conjunction with among the liquid reaction tube #3, moved up and down fast 20 times, mix reagent and sample were placed 5～15 minutes;
7, take out test-strips, in Washing cup, clean (with the 3rd step).Note test-strips not being taken out from Washing cup;
8, test-strips was placed in Washing cup 5 minutes;
9, from Washing cup, take out test-strips, insert among the development liquid reaction tube #4, fast on, following motion 20 times, mix reagent and sample were placed 5 minutes;
10, take out test-strips, in Washing cup, clean (with the 3rd step);
11, take out after cleaning is finished and keep flat (about 20～25 minutes) observations behind the reagent strip substantially dry.
[reference value]
Positive: the visible clear and well-defined spot in fenestra center;
Negative: it is very shallow to cannot see colour developing spot or spot in the fenestra, and boundary is fuzzy to be difficult for seeing.
Test-strips top fenestra is a positive control, must show the positive, and measurement result is just effective; The lowermost end fenestra is a negative control, must show feminine gender, and testing result is just effective.The reagent contrast is used for guaranteeing that each used reagent is that effectively wrong as any reagent results of comparison, then this time test must be reformed when each the analysis.
[explanation of assay]
Negative: as to illustrate that sample does not contain corresponding antibodies or antibody titer is very low;
Positive: showing has corresponding antibodies to exist.
The sample that method, haemolysis are put in the incorrect preservation of kit may exert an influence to the result.When positive findings occurring, must comprehensively judge in conjunction with the clinical practice situation, can adopt the additive method contrast to carry out in case of necessity.
Be divided into IgG and two kinds of models of IgM according to the asynchronism(-nization) of antibody appearance.
Kit of the present invention is disposable detection consumptive material, belongs to fast qualitative and detects.
This detection kit judges whether contain TORCH antibody in the detected sample for quick, and TORCH detects extremely important meaning, when being used to judge children's clinical infection type, can clinically select different therapeutic regimens according to different virus infections degree; When being used for checking before pregnant or pregnancy period when checking, can judge whether to exist the infection of TORCH virus, thereby reduce the occurrence rate of congenital inborn defect.
This kit is the specific immune response that utilizes antigen-antibody, can specificity be fixed on the immobilon-p in conjunction with the antigen of antiviral antibody, to measure sample joins in the reaction tube, insert reagent strip, different virus antibody is fixed on the film in the sample, add enzyme and chromogenic substrate again, can show blue purple dot if capture the position of antibody.
Can detect four kinds of antiviral antibodies simultaneously on the test-strips of this kit, the asynchronism(-nization) that occurs because of antibody is divided into IgG and two kinds of models of IgM.
Claims (8)
1. dry chemical method TORCH detection kit is characterized in that:
In the detecting unit of detection kit, comprise the matrix lath that is used to support reaction system;
The film material that is used for the adsorption reaction thing, this film material is fitted on the matrix lath;
Be attracted to the antigen on the film material, this antigen is with the some film device of accurate pipettor or robotization, and with the concentration of 1.0～3.0mg/ml, the amount bag of 1～3ul is by in the fenestra to the matrix lath; Described film material is nitrocellulose filter or PVDF membrane; Described antigen comprises arc worm, giant cell, rubella virus, herpes simplex antigen.
2. dry chemical method TORCH detection kit according to claim 1 is characterized in that: the reactant in the kit comprises dilution, strengthens liquid, in conjunction with liquid and development liquid; Reactant is used for specifically, and whether the detected sample of qualitative detection contains TORCH antibody; Described dilution comprises: 1～5% protein stabiliser and 0.01～0.1% Sodium azide, and surplus is the PB damping fluid, wherein the pH of damping fluid is 6.0～8.2; Described enhancing liquid includes 0.9～5.7% sodium chloride and 0.01～0.1% Sodium azide, Yu Weishui, and unit is a mass percent.
3. dry chemical method TORCH detection kit according to claim 1, it is characterized in that: described alkaline phosphatase, 1～5% protein stabiliser and 0.01～0.1% Sodium azide in conjunction with liquid bag and the anti-people's antibodies of animal, surplus is damping fluid, and the unit of protein stabiliser, Sodium azide and damping fluid is a mass percent; Described and the alkaline phosphatase anti-people's antibodies of animal is dissolved in the damping fluid, and the pH of damping fluid is 6.0～8.8, and the final concentration of alkaline phosphatase is 1～5mg/ml.
4. dry chemical method TORCH detection kit according to claim 1, it is characterized in that: described development liquid comprises that concentration is the chromogenic substrate of 0.5～3mg/ml, 0.01～0.1% Sodium azide, surplus is damping fluid, and wherein the unit of Sodium azide and damping fluid is a mass percent; It is in 8.8～11.0 the damping fluid that chromogenic substrate is dissolved in pH; Described chromogenic substrate is 5-bromo-4-chloro-3-indyl phosphate, a kind of in nitro tetrazolium chloride orchid, tetramethyl benzidine, the o-phenylenediamine.
5. dry chemical method TORCH detection kit according to claim 1 is characterized in that: also comprise being used for the disposable pipe that reacts for reagent strip and preventing moist drying agent in kit.
6. the manufacture method of the described dry chemical method TORCH of claim 1 detection kit, it is characterized in that: this method comprises following steps:
(1) preparation of reactant
A, preparation TORCH antigen; Described TORCH antigen comprises: toxoplasma antigen, rubella virus antigen, cytomegalovirus antigen, herpes simplex virus antigens;
B, the preparation anti-people's antibody of animal and alkaline phosphatase;
The anti-people's antibody of described animal comprises goat anti-human antibody, the anti-people's antibody of rabbit, the anti-people's antibody of horse or the anti-people's antibody of donkey;
C, preparation are used to support the lath of reaction system;
With of the dimensioned moulding of matrix lath, on the matrix lath, beat several reacting holes then according to reagent strip; The film material that will be used for the adsorption reaction thing again sticks on the back side of matrix lath, to cover the reacting hole on the lath;
(2) preparation of kit
The bag quilt and the processing of a, reactant
(a), the bag of TORCH antigen quilt and drying:
With ready TORCH antigen: toxoplasma antigen, giant cell antigen, rubella virus antigen, herpes simplex virus antigens are successively with pipettor or some film device, concentration with 1.0～3.0mg/ml, by in the reacting hole to the matrix lath, shady and cool place is dry with the amount bag of 1～3ul;
(b), bag is handled by the sealing of good reaction strip;
The matrix lath that bag is adsorbed soaked 0.5～1 hour with the lock solution of 5～20g/L, and the buffer solution for cleaning rear venting that takes out with 0.1M dries; Described lock solution comprises: the bovine serum albumin(BSA) of 1～5% mass concentration, 1.5～5% sucrose, 0.01～0.1% Sodium azide;
(c), the preparation of reactant
According to following proportioning preparation reactant: this reactant comprises dilution, strengthens liquid, in conjunction with liquid and development liquid;
Wherein, the consisting of of dilution: 1～5% protein stabiliser and 0.01～0.1% Sodium azide, surplus is damping fluid, the pH of described damping fluid is 6.0～8.2;
Strengthen liquid: contain 0.9～5.7% sodium chloride and 0.01～0.1% Sodium azide, Yu Weishui;
In conjunction with liquid: will be that 1～5% protein stabiliser and mass concentration are that to be dissolved in pH be in 6.0～8.8 the damping fluid for 0.01～0.1% Sodium azide with alkaline phosphatase, the mass concentration of the anti-people's antibodies of animal; Wherein, the mass concentration of protein stabiliser is 1～5%, and the mass concentration of Sodium azide is 0.01～0.1%, and surplus is damping fluid, and the final concentration of alkaline phosphatase is 1～5mg/ml;
Development liquid: the chromogenic substrate and the Sodium azide that will be dissolved in pH and be in 8.8～11.0 the damping fluid mix, and wherein, the concentration of chromogenic substrate is 0.5～3mg/ml, and the mass concentration of Sodium azide is 0.01～0.1%, and surplus is damping fluid; Described chromogenic substrate comprises 5-bromo-4-chloro-3-indyl phosphate, to nitro tetrazolium chloride orchid or tetramethyl benzidine, a kind of in the o-phenylenediamine;
The assembling of b, reagent strip and packing
(1) will seal dry matrix lath with hand papercutter and cut into reagent strip as required;
(2) reagent strip of well cutting is packed into plastic housing, the sealing moulding;
(3) reagent that will seal moulding carries out packing according to packing specification.
7. the manufacture method of dry chemical method TORCH detection kit as claimed in claim 6 is characterized in that: described toxoplasma antigen is T.gondii, the RH strain; Described rubella virus antigen is the HPV-77 strain; Described cytomegalovirus antigen is the AD-169 strain; Described herpes simplex virus antigens is the Maclntyre strain.
8. the manufacture method of dry chemical method TORCH detection kit as claimed in claim 6 is characterized in that: described damping fluid is phosphate buffer or phosphate buffer or acetate buffer solution.
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| CN101216490B CN101216490B (en) | 2012-05-30 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103018443A (en) * | 2012-12-15 | 2013-04-03 | 北京金豪制药股份有限公司 | IgG antibody detection kit of TORCH five items and preparation of kit |
| CN103033612A (en) * | 2012-12-15 | 2013-04-10 | 北京金豪制药股份有限公司 | IgM antibody detection kit for five TORCH tests and preparation of IgM antibody detection kit |
| CN103399149A (en) * | 2013-08-22 | 2013-11-20 | 青岛中仁药业有限公司 | Family-planning four-item combined kit employing enzyme linked immunosorbent spot assay |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1987461B (en) * | 2006-12-12 | 2011-09-28 | 盛青松 | Dry chemical quick detecting reagent strip for glutamic-pyruvic transaminase and its producing method |
| CN101038258A (en) * | 2007-04-02 | 2007-09-19 | 上海和盛生物科技有限公司 | Qualitative testing kit with dry chemical method and manufacturing method thereof |
| CN101074954A (en) * | 2007-06-07 | 2007-11-21 | 盛青松 | Chlorhydric-acid clenbuterol fast inspection reagent kit and its production |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103018443A (en) * | 2012-12-15 | 2013-04-03 | 北京金豪制药股份有限公司 | IgG antibody detection kit of TORCH five items and preparation of kit |
| CN103033612A (en) * | 2012-12-15 | 2013-04-10 | 北京金豪制药股份有限公司 | IgM antibody detection kit for five TORCH tests and preparation of IgM antibody detection kit |
| CN103399149A (en) * | 2013-08-22 | 2013-11-20 | 青岛中仁药业有限公司 | Family-planning four-item combined kit employing enzyme linked immunosorbent spot assay |
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Application publication date: 20080709 Assignee: Wuxi Shenrui Bio-Pharmaceuticals Co., Ltd. Assignor: Sheng Qingsong Contract record no.: 2013320000045 Denomination of invention: Dry chemical method TORCH detection reagent kit and method of manufacture Granted publication date: 20120530 License type: Exclusive License Record date: 20130220 |
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Effective date of registration: 20201224 Address after: 5 / F, c30303, C5, area C, Wuxi Huishan Life Science Park, 1699 Huishan Avenue, Huishan District, Wuxi City, Jiangsu Province Patentee after: WUXI SHENRUI BIO-PHARMACEUTICALS Co.,Ltd. Address before: 214058 Shen Rui Biological Products Co., Ltd., Huang Xiang hospital, 118 Fengxiang Road, Beitang District, Wuxi, Jiangsu Patentee before: Sheng Qingsong |