CN101216403B - Flue-cured tobacco leaf total cell wall substance content measuring method - Google Patents

Flue-cured tobacco leaf total cell wall substance content measuring method Download PDF

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CN101216403B
CN101216403B CN 200810049123 CN200810049123A CN101216403B CN 101216403 B CN101216403 B CN 101216403B CN 200810049123 CN200810049123 CN 200810049123 CN 200810049123 A CN200810049123 A CN 200810049123A CN 101216403 B CN101216403 B CN 101216403B
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total cell
microwave
cell wall
tobacco leaves
step
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CN 200810049123
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CN101216403A (en
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于建军
任晓红
刘学芝
史宏志
庞天河
杨永锋
焦桂珍
邵惠芳
陈刚
陈红丽
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河南农业大学
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Abstract

The invention discloses a method for determining content of total cell wall substances in tobacco leaves, which adopts microwave technology, and selects benzene-ethanol mixture solution as extractingsolvent and a refit soxhlet extractor as the extraction device. The method comprises the following steps of: making pulverized sample of tobacco leaves into a package; disposing the package in the refit soxhlet extractor containing benzene-ethanol mixture solution; placing in a refit microwave oven for extracting and heating treatment; adding distilled water for heating treatment; vacuum-filteringthe solvent containing the total cell wall substances and drying to obtain the total cell wall substances in the tobacco leaves; and calculating the content. Compared with the conventional determination method, the invention adopts the microwave technique to test the content of total cell wall substances in tobacco leaves, and has greatly shortened test time, simple operation, reduced drug consumption and more accurate test data by using the microwave technology.

Description

烤烟烟叶中总细胞壁物质含量的测定方法 Determination of substance content of tobacco leaves Total cell wall

一、 技术领域: First, the technical field:

[0001] 本发明涉及一种烤烟烟叶中某种物质含量的测定方法,特别是涉及一种烤烟烟叶 [0001] The present invention relates to a method for measuring substances in tobacco leaves in some, particularly to a tobacco leaves

中总细胞壁物质含量的测定方法。 Determination of the total material content of the cell wall.

二、 背景技术: Second, the technical background:

[0002] 烤烟烟叶干物质中总细胞壁物质的含量约为30%,在烟梗中的含量约为43. 8%, 高含量的烟草总细胞壁物质对烟草品质有着很大的不利影响。 DM [0002] The total content of flue-cured tobacco in the cell wall material is about 30%, the content in tobacco stems is about 43.8%, high levels of total tobacco cell wall material has a great negative impact on the quality of tobacco. 由于烤烟烟叶中总细胞壁物质含量的高低直接影响着烟草品质,为了筛选出质量合格的烟草,由此对烤烟烟叶中总细胞壁物质含量的测定方法的进一步研究是十分必要的。 As the tobacco leaves directly affect the level of total cell wall matter content of the tobacco quality, in order to filter out acceptable quality tobacco, thereby further research on the determination of total cell wall matter content of tobacco leaves it is necessary.

[0003] 目前,关于烤烟烟叶中总细胞壁物质含量的测定方法,现有技术已有研究,例如张槐苓,葛翠英,穆怀静等.烟草分析与检验[M].郑州:河南科学技术出版社,1994, 103-111. 在烟草分析与检验中主要公开了烤烟烟叶中总细胞壁物质含量的常规测定方法,该测定方法的主要内容是:以苯醇混合液为萃取溶剂,苯醇二者之间的配比比例为2 : l,以索式提取器为萃取装置,将烤烟烟叶样品先用苯醇混合液萃取48小时,再用热水继续处理24小时,在整个过程中需要多次更换萃取溶剂,需经过多次抽滤和清洗。 [0003] Currently, the tobacco leaves on determination of total cell wall matter content, the prior art has been studied, such as Huai Zhang Ling, Ge Cuiying, Muhuai Jing and other tobacco analysis and testing [M]. Zhengzhou: Henan Science and Technology Press . LTD., 1994, 103-111 in tobacco primary analysis and inspection discloses a conventional method for measuring the substance content in tobacco leaves of the total cell wall, the main content of this measurement method are: a mixture of benzene alcohol as the extraction solvent, both benzyl alcohol ratio is the ratio between 2: l, in a Soxhlet extractor as the extraction means, before the tobacco leaves with a mixture of benzyl alcohol samples were extracted for 48 hours and then hot water continued for 24 hours, the entire process requires multiple replace the extraction solvent, subject to suction filtration and washed several times. 由此看来常规的测定方法费时、耗力,浪费药品,并且所测实验结果也不理想。 From this conventional method for measuring time-consuming, labor-intensive, pharmaceutical waste, and the measured results are not ideal. 经过研究分析可知:加热方法是造成上述问题的主要原因。 After the study analysis shows that: the heating method is the main reason contributing to this problem. 为了解决上述问题,本发明将微波技术应用到烤烟烟叶中总细胞壁物质含量的测定方法这一新的领域中,经试验验证,效果非常理想。 To solve the above problems, the present invention is the application of microwave technology to this new method for measuring field of tobacco leaves matter content of total cell walls, the verification test, the effect is very desirable.

三、 发明内容: III SUMMARY OF THE INVENTION:

[0004] 本发明的目的是针对现有技术中存在的问题,提供一种操作简单、节约时间、所得 [0004] The object of the present invention is directed to the problems in the prior art, providing a simple, time saving, resulting

数据精确的烤烟烟叶中总细胞壁物质含量的测定方法。 Determination of total cell wall matter content in tobacco leaves accurate data.

[0005] 本发明的技术方案是: [0005] aspect of the present invention is:

[0006] 本发明提供一种烤烟烟叶中总细胞壁物质含量的测定方法,该测定方法以苯醇混合液为萃取溶剂,其详细步骤如下: [0006] The present invention provides a method for measuring substances in tobacco leaves in total cell wall, the assay method is a mixture of an alcohol benzene extraction solvent, the detailed steps are as follows:

[0007] a、称取一定量经过预处理的烤烟烟叶的粉碎样品,装入叠好的滤纸包内制成烟包; [0007] a, weighed amount of crushed samples pretreated flue-cured tobacco, the cigarette made of folded filter paper bag is loaded;

[0008] b、将配制好的苯醇混合液加入经过改装的索式提取器中,然后将制成的烟包放入苯醇混合液萃取溶剂中,将索式提取器放入经过改装的微波炉中;粉碎样品与苯醇混合液二者之间的配比比例为lg : 38〜42ml ;所述经过改装的索式提取器是将索式提取器底部圆底烧瓶改为平底矩形烧瓶,矩形烧瓶上方直接连接冷凝管;所述改装的微波炉是在微波炉上方钻孔,孔径与冷凝管下端外直径相匹配; [0008] b, the prepared mixture was added benzyl alcohol after Soxhlet extractor modified, and then made into cigarette alcohol benzene extraction solvent mixture, the Soxhlet extractor into a converted microwave; ratio ratio therebetween pulverized sample with a mixture of benzyl alcohol lg: 38~42ml; the elapsed modified soxhlet extraction is a Soxhlet extractor to the bottom of the round bottom flask was replaced by a rectangular flat-bottomed flask, the flask is connected directly above the rectangular condenser; a modified microwave oven is a microwave oven above the bore, the outer diameter of the lower end of the aperture to match with the condenser;

[0009] c、开启微波炉进行萃取和微波加热处理,该处理过程中微波炉的功率为160〜 220w,加热时间为25〜35min ; [0009] c, open the microwave oven and the microwave heating extraction, processing power of the microwave oven is 160~ 220w, the heating time is 25~35min;

[0010] d、萃取加热处理后加入蒸馏水再进行微波加热处理,粉碎样品与蒸馏水二者之间的配比比例为lg : 38〜42ml,该处理过程中微波炉的功率为220〜260w,加热时间为15〜25min ; [0010] d, distilled water was added and extracted after the heat treatment is microwave heating then, the ratio of the ratio between the two samples and distilled water was pulverized lg: 38~42ml, the power of the microwave process is 220~260w, heating time as 15~25min;

[0011] e、萃取处理后将含有总细胞壁物质的溶剂进行抽滤,然后将其放入烘箱进行干燥,干燥温度为75〜85°C ,干燥时间为8〜12小时,干燥后即得烤烟烟叶中总细胞壁物质, 称其重量,最后计算其含量(合格的烤烟烟叶中总细胞壁物质物质的含量为25〜35% )。 [0011] e, after the solvent extraction process containing the total cell wall material subjected to suction filtration, and then placed in an oven dried, the drying temperature is 75~85 ° C, the drying time of 8~12 hours, and dried to obtain Tobacco total tobacco cell wall material, weigh it, and finally calculate the content (content of tobacco leaves passing the total cell wall substance material is 25~35%).

[0012] 在上述的烤烟烟叶中总细胞壁物质含j [0012] j is the total cell wall material containing tobacco leaves in the above-described

粉碎样品的细度为180〜220目。 The fineness of ground sample 180~220 mesh.

[0013] 在上述的烤烟烟叶中总细胞壁物质含j [0013] j is the total cell wall material containing tobacco leaves in the above-described

苯醇混合液的最佳配比比例为lg : 40ml。 The best ratio is the ratio of benzyl alcohol mixture lg: 40ml.

[0014] 在上述的烤烟烟叶中总细胞壁物质含〗 [0014] Total cell wall material contained in the above〗 in tobacco leaves

佳功率为180w,最佳加热时间为30min。 Good power 180w, optimum heating time was 30min.

[0015] 在上述的烤烟烟叶中总细胞壁物质含j [0015] j is the total cell wall material containing tobacco leaves in the above-described

:的测定方法中,所述步骤a中烤烟烟叶的 : Measuring method, the step a of tobacco leaves

:的测定方法中,所述步骤b中粉碎样品与 : Measurement method, the sample was pulverized and step b

:的测定方法中,所述步骤c中微波炉的最 : Measurement method, said step c microwave most

:的测定方法中,所述步骤d中粉碎样品与 : Measurement method, the sample in step d pulverization

蒸馏水二者之间的最佳配比比例为lg : 40ml,微波炉的最佳功率为240w,最佳加热时间为20min。 Optimum ratio is the ratio between the two distilled lg: 40ml, the optimum power of the microwave oven 240w, optimum heating time was 20min.

[0016] 在上述的烤烟烟叶中总细胞壁物质含量的测定方法中,所述步骤e中抽滤时采用砂芯漏斗。 [0016] In the tobacco leaves Method for determination of total cell wall substance content using frit funnel filtration when the step e.

[0017] 在上述的烤烟烟叶中总细胞壁物质含量的测定方法中,所述苯醇混合液中二者之间的混合比例与常规测定方法中苯醇混合液的混合比例相同。 [0017] In the tobacco leaves Method for determination of total cell wall material content, the same ratio of benzene - ethanol mixture was mixed with a conventional method for measuring therebetween benzene methanol mixture, the mixing ratio.

[0018] 在烤烟烟叶中总细胞壁物质含量的常规测定方法中,采用的苯醇混合液二者之间 [0018] The conventional method for measuring the substance content in the total cell wall, between the two benzene alcohol mixture employed in tobacco leaves

的配比比例为2 : 1。 Ratio is the ratio of 2: 1.

[0019] 本发明的积极有益效果: [0019] The positive and beneficial effects of the present invention:

[0020] 1、试验时间大大縮短 [0020] 1, the test time is shortened

[0021] 在烤烟烟叶中总细胞壁物质含量的常规测定方法中,苯醇混合液处理阶段需要48 小时,水处理阶段需要24小时;而本发明的测定方法中苯醇混合液处理阶段只需30min左右,水处理阶段需要20min左右;由此看来本发明与现有技术相比大大縮短了试验时间。 [0021] Total cell wall of a conventional method for measuring the substance content, benzene alcohol mixture treatment stages required tobacco leaves for 48 hours, 24 hours treatment stage; the method of the present invention, benzene alcohol mixture treatment stages just 30min so, treatment stage takes about 20min; In view of the present invention over the prior art greatly reducing the test time. [0022] 2、操作简单 [0022] 2, the operation is simple

[0023] 在烤烟烟叶中总细胞壁物质含量的常规测定方法中,需要多次更换料液,并且需经过多次抽滤和清洗,操作复杂,且易被高温料液烫伤;而本发明的测定方法中由于采用了微波技术,只需更换3〜5次料液,并且操作中没有被料液烫伤的危险。 [0023] tobacco leaves in a conventional method for measuring the total cell wall substance content, the feed solution needs to be replaced several times, and is subject to suction filtration and washed several times, complicated operation, and easily feed high-temperature scalding liquid; measured according to the invention As a result of the method of microwave technology, only three to five times to replace the liquid material, and the operation is no danger of the liquid material burns. [0024] 3、本发明测定方法与常规方法相比节约药品 [0024] 3. The method of the present invention as compared with the conventional method of saving drugs

[0025] 常规方法中苯醇混合液处理阶段和水处理阶段都比本发明测定方法所需时间长, [0025] benzyl alcohol mixture in a conventional manner and processing stage than the water phase measuring method of the present invention takes a long time,

并且常规方法中料液的更换次数、抽滤和清洗的次数都比较多。 And the number of replacement, the number of suction filtration and washing in a conventional manner more than feed liquid. 因此,与本发明的测定方 Accordingly, the present invention is the measuring side

法相比,常规的测定方法所需混合液和蒸馏水以及其它药品都比本发明测定方法所需的量 Comparison method, the required amount of distilled water and a mixture of drugs and other than the method of the present invention required a conventional method for measuring

多,由此以来,本发明将微波技术应用到该测定方法中大大节约了药品。 More, since thereby, the present invention is applied to the microwave technology measurement method greatly saving drugs.

[0026] 4、利用本发明测定方法所得烤烟烟叶中总细胞壁物质含量的试验数据更准确:因 [0026] 4, resulting substance content measured by the method of total tobacco leaves the cell wall of the present invention, more accurate test data: because

为常规测定方法中利用的是水浴加热,热量太低不足以打断结合力较强的一些化学键,造 A conventional measuring method using a water bath is heated, the heat is too low to break chemical bonds of some stronger binding force, making

成反应不彻底;而本发明采用微波技术来测定烤烟烟叶中总细胞壁物质的含量,其中微波 As the reaction is not complete; the present invention to determine the content of total tobacco leaves the cell wall material using microwave technology, wherein the microwave

加热(微波频率高,极性分子摆动速度快,加热迅速)可以打断结合力较强的化学键,使反 Heating (high-frequency microwave, polar molecules swing speed, rapid heating) may be interrupted by a chemical bond stronger binding force of the trans

应更彻底,最后所测的总细胞壁物质含量的数据更准确。 Should be more thoroughly, the total data last cell wall substance content measured more accurately.

4四、具体实施方式: 4 IV DETAILED DESCRIPTION:

[0027] 以下实施例仅为了进一步说明本发明,而不是限制本发明的内容。 [0027] The following examples merely illustrate the invention further content, and not to limit the present invention.

[0028] 实施例一:一种烤烟烟叶中总细胞壁物质含量的测定方法 [0028] Example a: method for measuring the total material content of the cell wall Tobacco Tobacco

[0029] 本发明测定方法采用苯醇混合液作为萃取溶剂,其详细步骤如下: [0029] The method of the present invention, employing benzyl alcohol as the extraction solvent mixture, the detailed steps are as follows:

[0030] a、称取5g经过预处理的烤烟烟叶的粉碎样品,其样品的细度为180〜220目,装 [0030] a, 5g crushed weighed sample of tobacco Tobacco pretreatment, its fineness sample was 180~220 mesh, means

入叠好的滤纸包内制成烟包; Made of filter paper folded into the cigarette packet;

[0031] b、称取配制好的苯醇混合液200ml加入经过改装的索式提取器中,然后将制成的烟包放入苯醇混合液萃取溶剂中,将索式提取器放入经过改装的微波炉中;所述苯醇混合液中二者之间的混合比例与常规测定方法中苯醇混合液的混合比例相同;经过改装的索式提取器是将索式提取器底部圆底烧瓶改为平底矩形烧瓶,矩形烧瓶上方直接连接冷凝管; 改装的微波炉是在微波炉上方钻孔,孔径与冷凝管下端外直径相匹配; [0031] b, benzyl alcohol weighed formulated mixture was added to 200ml of a modified Soxhlet extractor, and then made into cigarette benzene alcohol extraction solvent mixture, the cable passes into the extractor modified microwave oven; benzene-alcohol mixture in the mixing ratio between the two conventional methods of measuring the mixing ratio of the mixed solution of benzene same alcohol; a modified Soxhlet extractor is the bottom of a round bottom flask Soxhlet extractor a rectangular flat-bottomed flask was replaced, the flask was connected directly to the top of the rectangular condenser; modified microwave oven is a microwave oven above the bore, the outer diameter of the lower end of the aperture to match with the condenser;

[0032] c、开启微波炉进行萃取和微波加热处理,该处理过程中微波炉的功率为180w,加热时间为30min ; [0032] c, open the microwave oven and the microwave heating extraction, the processing power of 180W microwave oven, the heating time for 30 min;

[0033] d、萃取加热处理后加入200ml蒸馏水再进行微波加热处理,粉碎样品与蒸馏水二者之间的配比比例为lg : 40ml,该处理过程中微波炉的功率为240w,加热时间为20min ; [0034] e、萃取处理后将含有总细胞壁物质的溶剂采用砂芯漏斗进行抽滤,然后将其放入烘箱进行干燥,干燥温度为75〜85t:,干燥时间为8〜12小时,干燥后所得烤烟烟叶中总细胞壁物质重量为1. 49g,计算其含量为29. 8 % 。 [0033] d, 200ml distilled water was added and extracted further heat treatment is microwave heating, the ratio of the ratio between the two samples and distilled water as pulverized lg: 40ml, the power of the microwave process is 240W, the heating time is 20min; [0034] e, after the solvent extraction process containing the total cell wall material using a frit funnel by suction filtration, and then placed in an oven for drying, a drying temperature of 75~85t :, drying time of 8~12 hours, and dried the resulting cell wall material tobacco leaves total weight of 1. 49g, calculated in an amount of 29.8%.

[0035] 实施例二:同实施例一相同之处不再叙述,不同之处在于: [0036] 步骤b中称取配制好的苯醇混合液萃取溶剂为195ml ; [0035] Example II: similarities with the first embodiment will not be described, except that: [0036] Step b weighed formulated benzyl alcohol extraction solvent is a mixture of 195 ml;

[0037] 步骤c萃取和微波加热处理过程中微波炉的功率为170w,加热时间为32min ; [0038] 步骤d中加入的蒸馏水的量为205ml,该处理过程中微波炉的功率为250w,加热时间为18min ; [0037] Step c was extracted and microwave processing power of the microwave oven is 170w, the heating time was 32min; amount [0038] Step d was added distilled water to 205ml, the power of the process of the microwave oven is 250w, the heating time is 18min;

[0039] 步骤e中干燥后所得烤烟烟叶中总细胞壁物质重量为1.32g,计算其含量为26. 4%。 [0039] Step-e After drying the resulting cell wall material tobacco leaves total weight of 1.32g, calculated in an amount of 26.4%.

[0040] 实施例三:同实施例一相同之处不再叙述,不同之处在于: [0041] 步骤b中称取配制好的苯醇混合液萃取溶剂为190ml ; [0040] Example Three: similarities with the first embodiment will not be described, except that: [0041] Step b weighed formulated benzyl alcohol mixture was extracted 190 ml of solvent;

[0042] 步骤c萃取和微波加热处理过程中微波炉的功率为200w,加热时间为28min ; [0043] 步骤d中加入的蒸馏水的量为210ml,该处理过程中微波炉的功率为230w,加热时间为22min ; [0042] Step c was extracted and microwave processing power of the microwave oven is 200w, the heating time was 28min; amount [0043] Step d was added distilled water to 210ml, the power of the process of the microwave oven is 230w, the heating time is 22min;

[0044] 步骤e中干燥后所得烤烟烟叶中总细胞壁物质重量为1.26g,计算其含量为25. 2%。 [0044] Step-e After drying the resulting cell wall material tobacco leaves total weight of 1.26g, calculated in an amount of 25.2%.

[0045] 实施例四:同实施例一相同之处不再叙述,不同之处在于: [0046] 步骤b中称取配制好的苯醇混合液萃取溶剂为210ml ; [0045] Example IV: similarities with the first embodiment will not be described, except that: [0046] Step b weighed formulated benzyl alcohol mixture was extracted 210 ml of solvent;

[0047] 步骤c萃取和微波加热处理过程中微波炉的功率为220w,加热时间为25min ; [0048] 步骤d中加入的蒸馏水的量为190ml,该处理过程中微波炉的功率为220w,加热时间为25min ; [0047] Step c was extracted and microwave processing power of the microwave oven is 220w, the heating time was 25min; amount [0048] Step d was added distilled water to 190ml, the power of the process of the microwave oven is 220w, the heating time is 25min;

[0049] 步骤e中干燥后所得烤烟烟叶中总细胞壁物质重量为1.58g,计算其含量为31. 6%。 [0049] Step-e After drying the resulting cell wall material tobacco leaves total weight of 1.58g, calculated in an amount of 31.6%.

5[0050] 实施例五:同实施例一相同之处不再叙述,不同之处在于: 5 [0050] Example V: similarities with the first embodiment will not be described, except that:

[0051] 步骤b中称取配制好的苯醇混合液萃取溶剂为205ml ; [0051] Step b weighed formulated benzyl alcohol extraction solvent is a mixture of 205 ml;

[0052] 步骤c萃取和微波加热处理过程中微波炉的功率为160w,加热时间为35min ; [0052] Step c microwave extraction and processing power of the microwave oven is 160w, the heating time is 35min;

[0053] 步骤d中加入的蒸馏水的量为195ml,该处理过程中微波炉的功率为260w,加热时间为15min ; Amount of distilled water [0053] Step d was added to 195ml, the power of the microwave process is 260w, the heating time is 15min;

[0054] 步骤e中干燥后所得烤烟烟叶中总细胞壁物质重量为1.63g,计算其含量为 [0054] Step-e After drying the resulting cell wall material tobacco leaves total weight of 1.63g, calculated in an amount of

32. 6%。 32.6%.

[0055] 实施例六:同实施例一相同之处不再叙述,不同之处在于: [0055] Example VI: similarities with one embodiment not described embodiment, except that:

[0056] 步骤b中称取配制好的苯醇混合液萃取溶剂为200ml ; [0056] Step b weighed formulated benzyl alcohol extraction solvent is a mixture of 200ml;

[0057] 步骤c萃取和微波加热处理过程中微波炉的功率为190w,加热时间为30min ; [0057] Step c microwave extraction and processing power of the microwave oven is 190w, the heating time for 30 min;

[0058] 步骤d中加入的蒸馏水的量为195ml,该处理过程中微波炉的功率为240w,加热时间为18min ; Amount of distilled water [0058] Step d was added to 195ml, the power of the microwave process is 240w, the heating time is 18min;

[0059] 步骤e中干燥后所得烤烟烟叶中总细胞壁物质重量为1.51g,计算其含量为30. 2%。 [0059] Step-e After drying the resulting cell wall material tobacco leaves total weight of 1.51g, calculated in an amount of 30.2%.

[0060] 实施例七:同实施例一相同之处不再叙述,不同之处在于: [0060] Example VII: in common with one embodiment not described embodiment, except that:

[0061] 步骤b中称取配制好的苯醇混合液萃取溶剂为195ml ; [0061] Step b weighed formulated benzyl alcohol extraction solvent is a mixture of 195 ml;

[0062] 步骤c萃取和微波加热处理过程中微波炉的功率为180w,加热时间为30min ; [0062] Step c microwave extraction and processing power of the microwave oven is 180w, the heating time for 30 min;

[0063] 步骤d中加入的蒸馏水的量为205ml,该处理过程中微波炉的功率为240w,加热时间为20min ; The amount of [0063] Step d of distilled water was added to 205ml, the power of the microwave process is 240w, the heating time is 20min;

[0064] 步骤e中干燥后所得烤烟烟叶中总细胞壁物质重量为1.43g,计算其含量为28. 6%。 [0064] Step-e After drying the resulting cell wall material tobacco leaves total weight of 1.43g, calculated in an amount of 28.6%.

[0065] 实施例八:同实施例一相同之处不再叙述,不同之处在于: [0065] Example VIII: with a similarities embodiment will not be described in Example, except that:

[0066] 步骤b中称取配制好的苯醇混合液萃取溶剂为205ml ; [0066] Step b weighed formulated benzyl alcohol extraction solvent is a mixture of 205 ml;

[0067] 步骤c萃取和微波加热处理过程中微波炉的功率为185w,加热时间为30min ; [0067] Step c microwave extraction and processing power of the microwave oven is 185w, the heating time for 30 min;

[0068] 步骤d中加入的蒸馏水的量为195ml,该处理过程中微波炉的功率为245w,加热时间为20min ; The amount of [0068] Step d of distilled water was added to 195ml, the power of the microwave process is 245w, the heating time is 20min;

[0069] 步骤e中干燥后所得烤烟烟叶中总细胞壁物质重量为1.69g,计算其含量为 [0069] Step-e After drying the resulting cell wall material tobacco leaves total weight of 1.69g, calculated in an amount of

33. 8%。 33.8%.

[0070] 实施例九:同实施例一相同之处不再叙述,不同之处在于: [0070] Embodiment 9: a similarities with the embodiment will not be described in Example, except that:

[0071] 步骤b中称取配制好的苯醇混合液萃取溶剂为195ml ; [0071] Step b weighed formulated benzyl alcohol extraction solvent is a mixture of 195 ml;

[0072] 步骤c萃取和微波加热处理过程中微波炉的功率为175w,加热时间为32min ; [0072] Step c microwave extraction and processing power of the microwave oven is 175w, the heating time is 32min;

[0073] 步骤d中加入的蒸馏水的量为205ml,该处理过程中微波炉的功率为235w,加热时间为22min ; The amount of [0073] Step d of distilled water was added to 205ml, the power of the microwave process is 235w, the heating time is 22min;

[0074] 步骤e中干燥后所得烤烟烟叶中总细胞壁物质重量为1.53g,计算其含量为30. 6%。 [0074] Step-e After drying the resulting cell wall material tobacco leaves total weight of 1.53g, calculated in an amount of 30.6%.

[0075] 实施例十:同实施例一相同之处不再叙述,不同之处在于: [0075] Example X: similarities with the first embodiment will not be described, except that:

[0076] 步骤b中称取配制好的苯醇混合液萃取溶剂为200ml ; [0076] Step b weighed formulated benzyl alcohol extraction solvent is a mixture of 200ml;

[0077] 步骤c萃取和微波加热处理过程中微波炉的功率为210w,加热时间为28min ; [0077] Step c microwave extraction and processing power of the microwave oven is 210w, the heating time is 28min;

[0078] 步骤d中加入的蒸馏水的量为205ml,该处理过程中微波炉的功率为240w,加热时[0079] 步骤e中干燥后所得烤烟烟叶中总细胞壁物质重量为1.45g,计算其含量为29. 0%。 [0078] The amount of distilled water added in step d is 205ml, the power of the microwave process is 240w, when heated [0079] Step-e After drying the resulting cell wall material tobacco leaves total weight of 1.45g, calculated in an amount of 29.0%.

Claims (6)

  1. 一种烤烟烟叶中总细胞壁物质含量的测定方法,该测定方法以苯醇混合液为萃取溶剂,其特征是:所述测定方法包括以下步骤:a、称取一定量经过预处理的烤烟烟叶的粉碎样品,装入叠好的滤纸包内制成烟包;b、将配制好的苯醇混合液加入经过改装的索式提取器中,然后将制成的烟包放入苯醇混合液萃取溶剂中,将索式提取器放入经过改装的微波炉中;粉碎样品与苯醇混合液二者之间的配比比例为1g∶38~42ml,所述经过改装的索式提取器是将索式提取器底部圆底烧瓶改为平底矩形烧瓶,矩形烧瓶上方直接连接冷凝管,所述改装的微波炉是在微波炉上方钻孔,孔径与冷凝管下端外直径相匹配;c、开启微波炉进行萃取和微波加热处理,该处理过程中微波炉的功率为160~220w,加热时间为25~35min;d、萃取加热处理后加入蒸馏水再进行微波加热处理,粉 Determination of total cell wall of one kind of flue-cured tobacco material content of tobacco, the assay method is a mixture of benzyl alcohol extraction solvent, characterized in that: said measuring method comprising the steps of: a, said certain amount of pre-processed tobacco leaves ground sample, the cigarette made of folded filter paper bag loaded; B, the prepared mixture was added benzyl alcohol, a modified Soxhlet extractor, and then the mixture was extracted into benzene produced alcohol cigarette solvent, the Soxhlet extractor into a converted microwave oven; ratio ratio therebetween pulverized sample with benzene-alcohol mixture is 1g:38 ~ 42ml, said a modified Soxhlet extractor is a cable the bottom of the extractor flat rectangular flask to a round bottom flask, the flask was connected directly to the top of the rectangular microwave condenser, the conversion is matched to the outer diameter of the borehole above the microwave oven, the lower end of the aperture and condenser; C, open the microwave oven and extracted microwave heating, microwave power of the process is 160 ~ 220w, the heating time is 25 ~ 35min; distilled water was added and then microwave heating treatment d, heat extraction process, powder 样品与蒸馏水二者之间的配比比例为1g∶38~42ml,该处理过程中微波炉的功率为220~260w,加热时间为15~25min;e、萃取处理后将含有总细胞壁物质的溶剂进行抽滤,然后将其放入烘箱进行干燥,干燥温度为75~85℃,干燥时间为8~12小时,干燥后即得烤烟烟叶中的总细胞壁物质,称其重量,最后计算含量。 The ratio between the ratio of the two sample and distilled water 1g:38 ~ 42ml, the power of the microwave process is 220 ~ 260w, the heating time is 15 ~ 25min; e, after the solvent extraction process containing the total cell wall material is suction filtered, then placed in an oven dried, the drying temperature is 75 ~ 85 ℃, the drying time is 8 to 12 hours, and dried to obtain the total cell wall material of the tobacco leaves, weigh it, and finally calculate the content.
  2. 2. 根据权利要求1所述的烤烟烟叶中总细胞壁物质含量的测定方法,其特征是:所述步骤a中烤烟烟叶的粉碎样品的细度为180〜220目。 The method for measuring the substance content in tobacco leaves according to claim 1, total cell walls, characterized in that: said step a fineness of the ground sample is 180~220 mesh flue-cured tobacco.
  3. 3. 根据权利要求1所述的烤烟烟叶中总细胞壁物质含量的测定方法,其特征是:所述步骤b中粉碎样品与苯醇混合液的最佳配比比例为lg : 40ml。 The method for measuring the substance content in tobacco leaves according to claim 1, total cell walls, characterized in that: said step b pulverized optimal proportion ratio of the sample mixture is an alcohol with benzene lg: 40ml.
  4. 4. 根据权利要求1所述的烤烟烟叶中总细胞壁物质含量的测定方法,其特征是:所述步骤c中微波炉的最佳功率为180w,最佳加热时间为30min。 The method for measuring the substance content in tobacco leaves according to claim 1, total cell walls, characterized in that: said step (c) is the optimum power 180W microwave oven, optimum heating time was 30min.
  5. 5. 根据权利要求1所述的烤烟烟叶中总细胞壁物质含量的测定方法,其特征是:所述步骤d中粉碎样品与蒸馏水二者之间的最佳配比比例为lg : 40ml,微波炉的最佳功率为240w,最佳加热时间为20min。 The method for measuring the substance content in tobacco leaves according to claim 1, total cell walls, characterized in that: said step d pulverizing ratio optimum ratio between the two samples and distilled water to lg: 40ml, microwave optimum power is 240w, optimum heating time was 20min.
  6. 6. 根据权利要求1所述的烤烟烟叶中总细胞壁物质含量的测定方法,其特征是:所述步骤e中抽滤时采用砂芯漏斗。 The method for measuring the substance content in tobacco leaves according to claim 1, total cell walls, characterized in that: said step e frit funnel using suction filtration time.
CN 200810049123 2008-01-21 2008-01-21 Flue-cured tobacco leaf total cell wall substance content measuring method CN101216403B (en)

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US5803081A (en) 1996-06-28 1998-09-08 Regent Court Technologies Tobacco and related products
CN1438843A (en) 2000-03-10 2003-08-27 英美烟草(投资)有限公司 Tobacco treatment

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