CN101210220A - Micro-fluid separation chip - Google Patents
Micro-fluid separation chip Download PDFInfo
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- CN101210220A CN101210220A CNA2006101615564A CN200610161556A CN101210220A CN 101210220 A CN101210220 A CN 101210220A CN A2006101615564 A CNA2006101615564 A CN A2006101615564A CN 200610161556 A CN200610161556 A CN 200610161556A CN 101210220 A CN101210220 A CN 101210220A
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Abstract
A microfluidic separation chip relates to the field of medical technology, the field of microorganism technology as well as the field of printing technology. The microfluidic separation chip comprises a horizontal constant-diameter main passage in straight pipe shape, and a tubular separation passage arranged below the main passage, wherein the separation passage has identical inner cross section areas at both ends; the middle section of the separation passage is communicated with the middle section of the main passage to form a communicated passage in straight pipe shape; and the communicated passage has a constant inner cross section, a length of 500 to 10,000 mum and an inner vertical length of 5 to 1,000 mum. By using laminar flow principle, the invention can separate active sperms from conventional sperm sample and from a sample that is difficult or unlikely to be separated by the conventional sperm separation method.
Description
Technical field
The present invention relates to field of medical technology, also relate to microbial technology field, also relate to printing technology.
Background technology
Have 10% Mr. and Mrs that the problem of infertile aspect is arranged approximately, wherein have 30%～40%, sperm or sperm are undesired to be caused by lacking.Effective artificial selection process makes successfully, and pregnancy, fertility and healthy offspring's possibility maximization is very important.At present, for this infertile state-of-the-art methods of treatment relevant with the male sex is exactly a kind of technology in vitro fertilization that is claimed the sperm injection in the inner cell slurry, make the ovum fertilization in this technology by the direct injection sperm, and this technical process has significantly reduced the quantity of survival sperm, and ovum fertilization probability is significantly reduced.Though each ovocyte only needs a sperm in theory, make the feasibility of finding out the sperm that viability is arranged most still have a challenge with present technology (handle separate as centrifugal) with free method, perhaps be still impossible in some cases.Like this, the doctor normally by hand Necrospermia and cell debris find one " good sperm " (as, have most motility and outstanding structure), promptly from the few sample of sperm of quantity or in a small amount of frozen semen (as, in the seminal fluid of preserving before the chemotherapy of patients) isolate the sperm of survival, this process need a few hours just may finish.Therefore from the lower concentration sperm sample of infertile patients or a spot of frozen sperm sample, isolate viable sperm and have clinically high value.
Summary of the invention
The object of the invention is to invent a kind of convenience is isolated viable sperm from the lower concentration sperm sample of infertile patients or a spot of frozen sperm sample micro-fluid separation chip.
The present invention includes the isometrical straight tube-like main channel of a level, arranged beneath tubulose split tunnel in the main channel, the two ends of described split tunnel have identical interior cross-sectional area, the middle part of described split tunnel is communicated with the middle part of main channel, the straight tube-like communication passage that forms has identical interior cross section, the length of described communication passage is 500～10000 μ m, and vertical height is 5～1000 μ m in the communication passage.
The present invention utilizes lamina flow principle, and screened sample and diluting soln are added with separately speed from the homonymy of main channel and split tunnel respectively, forms upper and lower two media stream in communication passage.When having certain stable flow velocity in the main channel, the dead essence of no kinetic energy, remains, and active low sperm then flows to the other end motion of main channel with top dielectric, the high quality viable sperm with kinetic energy is wherein then shaken off top dielectric stream, to lower floor's medium flow motion, these high quality viable sperm are under the flow velocity effect of layer dielectric stream simultaneously, and the other end motion to split tunnel realizes the screening of viable sperm.The present invention can be difficult to active sperm maybe can not carry out separating the isolating sample from conventional seminal fluid sample and with traditional sperm separation method.
The present invention can be with active sperm from separating fast and simply traditional reluctant tiny sampler of sperm isolation technique.The present invention is based on active sperm and have positive kinetic energy, can pass through the ability of the fairing in the stratified fluid, realized the purpose that active sperm is separated from non-active sperm and other cell debris.Whole separation screening process can not cause the dead essence that takes place because of separation, the present invention can satisfy the isolating needs of small sperm in the clinical medicine, and also the biological assay of testing for the motility that comprises family active sperm based on motility of sperm has been opened convenience.The present invention can be applicable to medical field widely, can also be applied to other Screening of Bioflocculant-producing Bacteria.
The two ends of main channel of the present invention are respectively sample channel and sieve from passage, in order to guarantee to form stable upper strata flow velocity in the main channel, sample channel is identical with the cross section of sieve in passage, and, sample channel and sieve in passage vertical height less than vertical height in the communication passage.
The two ends of split tunnel of the present invention are respectively solution channel and screening passage, in like manner, in order to guarantee to form stable lower floor's flow velocity in the split tunnel, solution channel is identical with cross section in the screening passage, and in solution channel and the screening passage vertical height less than vertical height in the communication passage.
Among the present invention, the interior cross section of communication passage is a rectangle, and sample channel, sieve are respectively rectangle from the interior cross section of passage, solution channel and screening passage.Each passage of orthogonal is further to guarantee streamlined formation, guarantees that high active sperm can flow to the motion of layer dielectric stream from top dielectric.
For convenience sample, diluting soln, two separated and collected devices are connected end of the present invention, from the outer end of passage and screening passage joint are set respectively at sample channel, solution channel, sieve.
In order to be more suitable for the separation screening to sperm, the sample channel among the present invention, solution channel, sieve vertical height in passage and screening passage is respectively 5～1000 μ m.
Description of drawings
Fig. 1 is a structural representation of the present invention.
Embodiment
As shown in Figure 1, the two ends of straight tube-like main channel 1 are respectively sample channel 1-1 and sieve from passage 1-2 partially, and main channel 1 is horizontally disposed.At the outer end of sample channel 1-1 jointing 3, sieving from the outer end of passage 1-2 jointing 4.
1 arranged beneath is converted into the inclined to one side tubulose split tunnel 2 of bridge shape in the main channel, and the two ends of split tunnel 2 are solution channel 2-1 and screening passage 2-2.At the outer end of solution channel 2-1 jointing 5, at the outer end jointing 6 of screening passage 2-2.
The middle part of split tunnel 2 is communicated with the middle part of main channel 1, and the interior cross section of the straight tube-like communication passage of formation is rectangular.
The length of communication passage can be 500～10000 μ m, and vertical height can be 5～1000 μ m in the communication passage.
Sample channel 1-1, solution channel 2-1, sieve vertical height in passage 1-2 and screening passage 2-2 can be 5～1000 μ m respectively.
In this example:
The pipe inner height H of sample channel 1-1
1Be 50 μ m, length L
1Be 500 μ m.
Sieve is from the pipe inner height H of passage 1-2
2Be 50 μ m, length L
2Be 500 μ m.
The pipe inner height H of solution channel 2-1
3Be 150 μ m, length L
3Be 2000 μ m.
The pipe inner height H of screening passage 2-2
4Be 150 μ m, length L
4Be 2000 μ m.
The pipe inner height H of communication passage is 200 μ m, and length L is 7000 μ m.
Make method of the present invention and can adopt soft lithography or laser lithography technology, passage is enclosed in by on the glass-faced lantern slide.
Use example:
1, respectively the collecting semen sample, prepare 1% BSA solution, staining of sperm agent PI.
2, connect:
The BSA solution of 60 μ L is added the solution dilution pond, the solution dilution pond is tightly connected by the lower end of joint 5 with solution channel 2-1.
The seminal fluid sample of washing of 50 μ L with after an amount of staining of sperm agent PI mixes, is added receiving flask and is tightly connected by joint 3 and sample channel 1-1.
The BSA solution of 2 μ L is added a separated and collected device, and this separated and collected device is sealedly connected on the outer end of sieve from passage 1-2 by joint 4.
Another separated and collected device is sealedly connected on the lower end of screening passage 2-2 by joint 6.
3, separate:
In the solution dilution pond, adopt positive pressure equipment, or with separated and collected device that screening passage 2-2 is connected in adopt negative pressure equipment, make to have certain stable velocity pressure in the main channel 1.
Positive pressure equipment is adopted in outer end at sample channel 1-1, or adopts negative pressure equipment from passage 1-2 outer end at sieve, makes to have certain stable velocity pressure in the split tunnel 2.
Microscope by phase contrast can be observed the part viable sperm from sample channel 1-1, swims to screening passage 2-2 through communication passage.
By above method just can with separated and collected device that split tunnel 2 is connected in collect activity, kinetic energy sperm.
Claims (6)
1. micro-fluid separation chip, it is characterized in that comprising the isometrical straight tube-like main channel of a level, arranged beneath tubulose split tunnel in the main channel, the two ends of described split tunnel have identical interior cross-sectional area, the middle part of described split tunnel is communicated with the middle part of main channel, the straight tube-like communication passage that forms has identical interior cross section, and the length of described communication passage is 500～10000 μ m, and vertical height is 5～1000 μ m in the communication passage.
2. according to the described micro-fluid separation chip of claim 1, the two ends that it is characterized in that the main channel are respectively sample channel and sieve from passage, sample channel is identical with the cross section of sieve in passage, and sample channel and sieve in passage vertical height less than vertical height in the communication passage.
3. according to the described micro-fluid separation chip of claim 2, the two ends that it is characterized in that split tunnel are respectively solution channel and screening passage, solution channel is identical with the cross section of screening in the passage, and in solution channel and the screening passage vertical height less than vertical height in the communication passage.
4. according to the described micro-fluid separation chip of claim 3, the interior cross section that it is characterized in that communication passage is a rectangle, and sample channel, sieve are respectively rectangle from the interior cross section of passage, solution channel and screening passage.
5. according to the described micro-fluid separation chip of claim 1, it is characterized in that from the outer end of passage and screening passage joint being set respectively at sample channel, solution channel, sieve.
6. according to claim 1 or 2 or 3 or 4 or 5 described micro-fluid separation chips, it is characterized in that described sample channel, solution channel, sieve vertical height in passage and screening passage is respectively 5～1000 μ m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006101615564A CN101210220A (en) | 2006-12-28 | 2006-12-28 | Micro-fluid separation chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006101615564A CN101210220A (en) | 2006-12-28 | 2006-12-28 | Micro-fluid separation chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101210220A true CN101210220A (en) | 2008-07-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2006101615564A Pending CN101210220A (en) | 2006-12-28 | 2006-12-28 | Micro-fluid separation chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101210220A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102242055A (en) * | 2011-06-03 | 2011-11-16 | 博奥生物有限公司 | Method for evaluating sperm activity and screening sperms and special microfluidic chip device for same |
| US11517900B2 (en) | 2017-10-27 | 2022-12-06 | University Of Utah Research Foundation | Microfluidic system for sperm separation and enrichment from various types of sperm samples |
-
2006
- 2006-12-28 CN CNA2006101615564A patent/CN101210220A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102242055A (en) * | 2011-06-03 | 2011-11-16 | 博奥生物有限公司 | Method for evaluating sperm activity and screening sperms and special microfluidic chip device for same |
| CN102242055B (en) * | 2011-06-03 | 2013-08-14 | 博奥生物有限公司 | Method for evaluating sperm activity and screening sperms and special microfluidic chip device for same |
| US11517900B2 (en) | 2017-10-27 | 2022-12-06 | University Of Utah Research Foundation | Microfluidic system for sperm separation and enrichment from various types of sperm samples |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20080702 |