CN101186655A - Method for extracting bacteria alginate - Google Patents
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- CN101186655A CN101186655A CNA2007101150557A CN200710115055A CN101186655A CN 101186655 A CN101186655 A CN 101186655A CN A2007101150557 A CNA2007101150557 A CN A2007101150557A CN 200710115055 A CN200710115055 A CN 200710115055A CN 101186655 A CN101186655 A CN 101186655A
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Abstract
The invention discloses an extraction method of bacterial alginate, which is characterized in composed of following steps as (1), cleaning and drying sludge, (2), arranging the dried sludge into Na2CO3 solution to be heated and dissolved, (3), cooling and filtering the heated product to obtain supernatant, (4), adding acid solution into the supernatant to adjust pH and obtain algin deposits, (5), dissolving the algin deposits into NaOH solution to obtain bacterial sodium alginate solution, (6), extracting via alcohol to obtain bacterial sodium alginate solid. The inventive method can extract bacterial alginate product from sludge with high economic benefits, which can reduce the final sludge discharge and reduce total operation cost of sewage treatment plant.
Description
Technical field
The present invention relates to a kind of method of extracting bacteria alginate, specifically, relate to a kind of method of from mud, extracting bacteria alginate, belong to the environmental engineering technical field of sewage.
Background technology
The mud that sewage work produces is one of the great secondary pollution of modern city source.Annual 1800000 tons of the dewatered sludges (9,000,000 tons in water ratio 80% mud) that produce of present national sewage work estimate that will produce 5,400,000 tons of dewatered sludges (2,700 ten thousand tons in water ratio 80% mud) every year in the coming five years.The processing costs of sludge of sewage treatment plant accounts for 20% to 45% of sewage work's overall operation expense, have in addition up to 60%.Therefore, how handling mud effectively, and it is become a kind of valuable renewable resource, is the problem that modern city presses for solution.
Studies show that bacterium exocellular polysaccharide material is a mud, as the main component of active sludge, microbial film, anaerobic grain sludge, is important polysaccharide fraction with bacteria alginate wherein.Bacteria alginate can be used as the potential surrogate of alginates, has a wide range of applications.As promote the extension and the root growth of plant germination and bud; Be used as biochemical fertilizer as the somatomedin of bifidus bacillus or on agricultural, promote the propagation and/or the differentiation of bifidus bacillus and some vegetable cells; After special processing as cancer therapy drug; Replace the Niu Chuiti extract as Urogastron, promote HE hyperplasia and differentiation; And can be used as metal absorbent and use.
Summary of the invention
The invention provides a kind of method of extracting bacteria alginate, utilize this method can from mud, extract bacteria alginate product with higher economic worth, the output of the final mud of sewage work not only can be reduced, and the overall operation expense of sewage work can be reduced.
For solving the problems of the technologies described above, the present invention is achieved by the following technical solutions:
A kind of method of extracting bacteria alginate is characterized in that comprising the steps:
(1) mud cleaning-drying:, and under 30-80 ℃, dry to constant weight with the mud washed with de-ionized water;
(2) Na
2CO
3Heating for dissolving in the solution: above-mentioned dried mud is placed Na
2CO
3Heated and stirred in the solution is until there being foam to overflow;
(3) cold filtration: the product after the above-mentioned heated and stirred is cooled to room temperature, uses filter cloth elimination residue then, obtain supernatant liquor;
(4) adjust pH: in supernatant liquor, splash into acid solution and regulate the pH value, make it be acid, obtain the alginic acid precipitation, centrifugal then collection alginic acid precipitation;
(5) dissolve in the NaOH solution: above-mentioned alginic acid resolution of precipitate in NaOH solution, is made the bacterium solution of sodium alginate.
(6) extraction using alcohol: above-mentioned bacterium solution of sodium alginate is mixed with dehydrated alcohol, collect flocks, 60-70 ℃ can make bacterium sodiun alginate solid after the oven dry down.
In the present invention, also have following technical characterictic: described mud is a kind of in active sludge, microbial film, aerobic particle mud and the anaerobic grain sludge.
In the present invention, also have following technical characterictic: splashing into acid solution in the described supernatant liquor, to transfer to the pH value be 2.
In the present invention, also have following technical characterictic: the described concentration that splashes into the acid solution in the supernatant liquor is 1-2mol/L.
In the present invention, also have following technical characterictic: the described acid solution that splashes in the supernatant liquor is HCL solution or H
2SO
4Solution.
In the present invention, also has following technical characterictic: described Na
2CO
3The concentration of solution is 0.1-1mol/L, and its weight is 50-200 times of sludge-drying weight.
In the present invention, also have following technical characterictic: the concentration of described NaOH solution is 0.1-1mol/L.
In the present invention, also have following technical characterictic: the volume of bacterium solution of sodium alginate is 0.5-2 a times of ethanol solution volume.
Compared with prior art, advantage of the present invention and positively effect are:
1, by from mud, extracting bacteria alginate, can reduce the final sludge quantity of discharging of sewage work, reduced the pollution of mud, and a kind of approach of effective processing mud is provided for sewage work environment.
2, utilize the method for the invention from mud, to extract bacteria alginate, can reduce the overall operation cost of sewage work with high added value.
3, the bacteria alginate that extracts from mud can replace the alginates that extracts from marine alga in certain applications, thereby reduced the consumption of marine algae resource, is beneficial to marine environmental protection.
4, it is simple that the present invention extracts the process of bacteria alginate, and medicine and energy consumption are lower, and cost is lower.
Embodiment
The present invention is further detailed explanation below in conjunction with embodiment.
With active sludge washed with de-ionized water three times, place loft drier under 30 ℃, to be dried to constant weight then.Getting the dried mud of 2g, to place 200ml, concentration be the Na of 0.2mol/L
2CO
3In the solution, heated and stirred is to there being foam to overflow.Use filter cloth elimination residue after being cooled to room temperature, obtain supernatant liquor.In supernatant liquor, splash into 10ml then, concentration is the HCL solution of 1mol/L, regulating pH value is 2, form the alginic acid precipitation, and precipitation is complete.Centrifugal collection alginic acid precipitation, and with its be dissolved in 100ml, concentration is in the NaOH solution of 0.1mol/L, can make the bacterium solution of sodium alginate.
In order to improve the purity of extract bacterium sodiun alginate, the above-mentioned bacterium solution of sodium alginate that makes is used filter cloth elimination residue once more, and splashing into concentration is the HCL solution of 1mol/L, and regulating the pH value is 2, centrifugal collecting precipitation is in the NaOH solution of 0.1mol/L with resolution of precipitate in 100ml, concentration again.Repeat above-mentioned filtration once more, transfer dissolved step in pH, collecting precipitation, the NaOH solution, regulating the pH value is 7, can make the bacterium solution of sodium alginate.With this bacterium algae sodium solution with 2: 1 (v: v) mix, collect flocks, can make bacterium sodiun alginate solid after 60 ℃ of oven dry with dehydrated alcohol.Finally can make 0.45g, pH value and be 7 bacteria alginate.
The above-mentioned principal reaction formula of extracting the bacterium sodiun alginate from mud is as follows:
Me(ALG)
n+Na
2CO
3→MeCO
3↓+nNaALG
NaALG+H
+→HALG↓+Na
+
HALG+NaOH→NaALG+H
2O
Wherein, ALG represents the alginic acid root, and Me is a metal ion.
Select for use the mud of other kinds to extract bacteria alginate according to the method described above, the amount of the alginate that various mud can extract is respectively: microbial film 282mg/g, anaerobic grain sludge 219mg/g, aerobic particle mud 351mg/g.
In the actual extracting process, because the composition of the mud of choosing in different places there are differences, the amount of the bacteria alginate that can extract in the mud can be different.
Certainly; above-mentioned explanation is not to be limitation of the present invention; the present invention also is not limited in above-mentioned giving an example, and variation, remodeling, interpolation or replacement that those skilled in the art are made in essential scope of the present invention also should belong to protection scope of the present invention.
Claims (8)
1. a method of extracting bacteria alginate is characterized in that comprising the steps:
(1) mud cleaning-drying:, and under 30-80 ℃, dry to constant weight with the mud washed with de-ionized water;
(2) Na
2CO
3Heating for dissolving in the solution: above-mentioned dried mud is placed Na
2CO
3Heated and stirred in the solution is until there being foam to overflow;
(3) cold filtration: the product after the above-mentioned heated and stirred is cooled to room temperature, uses filter cloth elimination residue then, obtain supernatant liquor;
(4) adjust pH: in supernatant liquor, splash into acid solution and regulate the pH value, make it be acid, obtain the alginic acid precipitation, centrifugal then collection alginic acid precipitation;
(5) dissolve in the NaOH solution: above-mentioned alginic acid resolution of precipitate in NaOH solution, is made the bacterium solution of sodium alginate.
(6) extraction using alcohol: above-mentioned bacterium solution of sodium alginate is mixed with dehydrated alcohol, collect flocks, 60-70 ℃ can make bacterium sodiun alginate solid after the oven dry down.
2. the method for extraction bacteria alginate according to claim 1 is characterized in that described mud is a kind of in active sludge, microbial film, aerobic particle mud and the anaerobic grain sludge.
3. the method for extraction bacteria alginate according to claim 1 and 2, it is characterized in that splashing in the supernatant liquor acid solution, to transfer to the pH value be 2.
4. the method for extraction bacteria alginate according to claim 3, the concentration that it is characterized in that splashing into the acid solution in the supernatant liquor is 1-2mol/L.
5. the method for extraction bacteria alginate according to claim 4, the acid solution that it is characterized in that splashing in the supernatant liquor is HCL solution or H
2SO
4Solution.
6. the method for extraction bacteria alginate according to claim 1 is characterized in that described Na
2CO
3The concentration of solution is 0.1-1mol/L, and its weight is 50-200 times of sludge-drying weight.
7. the method for extraction bacteria alginate according to claim 1, the concentration that it is characterized in that NaOH solution is 0.1-1mol/L.
8. the method for extraction bacteria alginate according to claim 1, the volume that it is characterized in that the bacterium solution of sodium alginate are 0.5-2 times of ethanol solution volume.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007101150557A CN100551933C (en) | 2007-11-26 | 2007-11-26 | A kind of method of extracting bacteria alginate |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007101150557A CN100551933C (en) | 2007-11-26 | 2007-11-26 | A kind of method of extracting bacteria alginate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101186655A true CN101186655A (en) | 2008-05-28 |
| CN100551933C CN100551933C (en) | 2009-10-21 |
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ID=39479299
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|---|---|---|---|
| CNB2007101150557A Expired - Fee Related CN100551933C (en) | 2007-11-26 | 2007-11-26 | A kind of method of extracting bacteria alginate |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103342756A (en) * | 2013-07-15 | 2013-10-09 | 湖南尔康制药股份有限公司 | Production technology of medical sodium alginate |
| CN104528929A (en) * | 2015-01-05 | 2015-04-22 | 江南大学 | Method for cultivating aerobic granular sludge quickly at low temperature |
| CN105585641A (en) * | 2016-03-04 | 2016-05-18 | 河海大学 | Method for extracting bacterial alginate from activated sludge |
| CN105693031A (en) * | 2016-03-31 | 2016-06-22 | 北京建筑大学 | Method and device for synthesizing alginates in sewage treatment process |
| CN108426880A (en) * | 2018-06-12 | 2018-08-21 | 南开大学 | A kind of method of bacteria alginate content in measurement sludge |
| CN108570115A (en) * | 2018-03-30 | 2018-09-25 | 北京建筑大学 | The recovery method of polysaccharide in a kind of extracellular polymeric |
| CN109153733A (en) * | 2016-03-16 | 2019-01-04 | 荷兰哈斯科宁德夫有限公司 | For obtaining the biopolymer extracting method of fibrous biopolymer, the fibrous biopolymer and fibrous alginates amide obtained with the method |
| CN109180831A (en) * | 2018-09-30 | 2019-01-11 | 薛蔚琦 | The method for extracting simultaneously purification of sulphuric acids polysaccharide as raw material using municipal sewage plant activated sludge |
| CN111003716A (en) * | 2019-12-17 | 2020-04-14 | 台州职业技术学院 | Comprehensive utilization method of seabed sludge |
| CN115073050A (en) * | 2022-06-02 | 2022-09-20 | 澳门大学 | Application of bacterial alginate extracted from activated sludge in concrete |
| CN115301204A (en) * | 2022-09-02 | 2022-11-08 | 浙江大学 | Residual sludge alginate magnetic nano-phosphorus adsorbent and preparation method thereof |
| CN115322342A (en) * | 2022-08-12 | 2022-11-11 | 哈尔滨工业大学 | Method for synchronously recycling polyhydroxyalkanoate and alginate in activated sludge in sequential manner |
-
2007
- 2007-11-26 CN CNB2007101150557A patent/CN100551933C/en not_active Expired - Fee Related
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103342756A (en) * | 2013-07-15 | 2013-10-09 | 湖南尔康制药股份有限公司 | Production technology of medical sodium alginate |
| CN103342756B (en) * | 2013-07-15 | 2014-07-23 | 湖南尔康制药股份有限公司 | Production technology of medical sodium alginate |
| CN104528929A (en) * | 2015-01-05 | 2015-04-22 | 江南大学 | Method for cultivating aerobic granular sludge quickly at low temperature |
| CN105585641A (en) * | 2016-03-04 | 2016-05-18 | 河海大学 | Method for extracting bacterial alginate from activated sludge |
| CN109153733A (en) * | 2016-03-16 | 2019-01-04 | 荷兰哈斯科宁德夫有限公司 | For obtaining the biopolymer extracting method of fibrous biopolymer, the fibrous biopolymer and fibrous alginates amide obtained with the method |
| CN105693031B (en) * | 2016-03-31 | 2018-09-18 | 北京建筑大学 | The method and equipment of alginates are synthesized in a kind of sewage disposal process |
| CN105693031A (en) * | 2016-03-31 | 2016-06-22 | 北京建筑大学 | Method and device for synthesizing alginates in sewage treatment process |
| CN108570115A (en) * | 2018-03-30 | 2018-09-25 | 北京建筑大学 | The recovery method of polysaccharide in a kind of extracellular polymeric |
| CN108426880A (en) * | 2018-06-12 | 2018-08-21 | 南开大学 | A kind of method of bacteria alginate content in measurement sludge |
| CN109180831A (en) * | 2018-09-30 | 2019-01-11 | 薛蔚琦 | The method for extracting simultaneously purification of sulphuric acids polysaccharide as raw material using municipal sewage plant activated sludge |
| CN111003716A (en) * | 2019-12-17 | 2020-04-14 | 台州职业技术学院 | Comprehensive utilization method of seabed sludge |
| CN115073050A (en) * | 2022-06-02 | 2022-09-20 | 澳门大学 | Application of bacterial alginate extracted from activated sludge in concrete |
| CN115322342A (en) * | 2022-08-12 | 2022-11-11 | 哈尔滨工业大学 | Method for synchronously recycling polyhydroxyalkanoate and alginate in activated sludge in sequential manner |
| CN115301204A (en) * | 2022-09-02 | 2022-11-08 | 浙江大学 | Residual sludge alginate magnetic nano-phosphorus adsorbent and preparation method thereof |
| CN115301204B (en) * | 2022-09-02 | 2024-01-02 | 浙江大学 | Residual sludge alginate magnetic nano phosphorus adsorbent and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100551933C (en) | 2009-10-21 |
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Granted publication date: 20091021 Termination date: 20111126 |