CN101156555B - a artificial fecundation method for Siberian sturgeon and hatching arrangement - Google Patents
a artificial fecundation method for Siberian sturgeon and hatching arrangement Download PDFInfo
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- CN101156555B CN101156555B CN2007100704461A CN200710070446A CN101156555B CN 101156555 B CN101156555 B CN 101156555B CN 2007100704461 A CN2007100704461 A CN 2007100704461A CN 200710070446 A CN200710070446 A CN 200710070446A CN 101156555 B CN101156555 B CN 101156555B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention provides an artificial Siberian sturgeon propagating method and a hatch device. The artificial propagating method includes six steps of selecting parent sturgeon, parent sturgeon breeding, artificial parturition facilitating, artificial fertilization, hatch and fry breeding. Lowering the water temperature artificially when breeding the parent sturgeon and injecting sturgeon pituitary can stimulate the gonad of the parent sturgeon to grow mature in advance. Artificial fertilization, hatch and fry breeding can solve the influence of limitation of the season and region on the Siberian sturgeon, and then the fine fry of the Siberian sturgeon can be provided stably and continuously to meet the available requirement for the fine sturgeon fry from the culturist all through the year. The hatch device includes a water providing pipe, a water inlet pipe and a water distributing pipe. An overflow opening is also arranged on the upside of the fry collecting pipe hatch bottle parallel arranged on the edge of the hatch bottle, a temporary feeding pond is arranged on the bottom of the lower end of the fry collecting pipe. The hatched fry can enter into the fry collecting pipe through the overflow opening, and then enter into the temporary feeding pond, and then the automatic fry collecting effect is realized, the invention dispenses with manual collecting as well as lowers the labor intensity greatly.
Description
Technical field
The present invention relates to a kind of fish culture technology, be specifically related to a kind of artificial fecundation method for Siberian sturgeon and hatching apparatus.
Background technology
The Siberia sturgeon is famous and precious economic fish, and is Fresh & Tender in Texture, nutritious, liked by the masses.But the Siberia sturgeon mainly perches in the river, Siberia, and China's wild Siberia sturgeon resource-constrained in order to satisfy the people's needs, presses for the Siberia sturgeon is propagated artificially.And existing Technique in Fishes cultural technique can only be carried out artificial insemination through extracting ovum and sperm in sexually matured naturally breeding period in every year; Then fertilized egg is placed in the incubator and hatches; Like the patent No. is in the patent of invention of CN1353927, and the environment of giant salamander artificial propagation and its natural propagation environment facies are seemingly; Existing fish incubation equipment comprises feed pipe, tank, water supply shower nozzle, support, Sheng ovum groove, drainage duct, dials egg apparatus and automatic tipping bucket.The water supply shower nozzle constantly supplies water in containing the ovum groove, and waste water is discharged from gap and got in the automatic tipping bucket, and after the water in the tipping bucket was full, instead with the water turned letter, tipping bucket erected water receiving again under the effect of the mass that resets automatically.Tipping bucket overturning and the action that erects drive simultaneously dials the motion of egg apparatus once reciprocating, dials egg apparatus and through the water of stirring the ovum dish ovum is stirred.In the hatching process that uses this incubation equipment, not in place if group egg apparatus is dialled ovum, fish-egg is easy to be deposited on contain in the ovum groove and causes anoxic to become dead ovum; The collection of fry must artificially be pulled out from containing in the ovum groove, and it is very big to receive the seedling workload; This device structure is complicated, and footprint is big, and complete set of equipments adopts the stainless steel material manufacturing, and the cost cost is very high, and this artificial culture technology is confined to the north, and the water temperature of south this moment can not satisfy the requirement of hatching.Therefore, existing Siberia sturgeon artificial seed hatching technique can't satisfy the demand of market to fry owing to receive the restriction of season and region.
Summary of the invention
The object of the present invention is to provide a kind of artificial fecundation method for Siberian sturgeon and hatching apparatus, use this artificial fecundation method for Siberian sturgeon and hatching apparatus to have following advantage;
1. can solve the influence that Siberia sturgeon seed is limited by season and region, stable, lasting supply Siberia sturgeon breeding satisfies the long-term effective demand of raiser to the sturgeon breeding.
2. fertilized egg is fully hatched.
3. can realize the automatic collection in the fry hatching process, reduce labor workload.
To achieve these goals, the technical scheme of the present invention's employing is: a kind of artificial fecundation method for Siberian sturgeon mainly may further comprise the steps:
One. select parent population at winter-spring season, from cultivating pool, fish for and select the system stalwartness, body surface does not have wound, movable normal of the right age male and female parent population;
Two. parent fish rearing
It is that cultivate in 6 ℃-10 ℃ cultivation pond that a puts into water temperature with parent population, and the water temperature that will cultivate after 30-37 days in the pond is progressively brought up to 14 ℃-18 ℃ by 6 ℃-10 ℃, improves every day about 1 ℃; Water quality is pure and fresh in the maintenance cultivation pond, and oxygen is sufficient, because the environment of low temperature stimulation artificially is provided to parent population, so just can do sth. in advance the sexually matured time of sturgeon;
B changes parent population in the hatchery over to after water temperature rises to 14 ℃-18 ℃, and keeping the water temperature in the hatchery is about 16 ℃, and the hatchery is in flow regime all the time, and the hatchery water velocity is controlled at 0.05 meter per second-0.15 meter per second;
Three. artificial induced spawning
A once hastens parturition and in the parent population body, injects the sturgeon hypophysis, and the injection volume of female sturgeon is a 1-16 mg/kg body weight, and male sturgeon reduces by half; Also can in the parent population body, inject carp brain hypophysis, female, male sturgeon injection volume is 5-7 mg/kg body weight;
The b secondary is hastened parturition and in the parent population body, is injected carp brain hypophysis, and the injection volume of female sturgeon is a 1-15 mg/kg body weight, and male sturgeon reduces by half; Under the high slightly situation of water temperature, can take the circumstances into consideration suitably to reduce to use dosage as the case may be, the dosage of injection is the 65%-90% of accumulated dose for the second time.
C puts back to parent population to cultivate in the pond and cultivates behind artificial induced spawning;
Four. artificial insemination is when estimating the parent population sexual maturity, and ripe essence, ovum are obtained in manual work; Obtain after essence, the ovum with manual method, carry out artificial insemination, fill fertilized egg pouring in the washbasin of clear water then, evenly stir 3～5 minutes after, the water that will contain seminal fluid is outwelled, and cleans several times with clear water;
Five. hatching is put into the hatching of hatching bottle with fertilized egg, and the control water temperature is 10 ℃-15 ℃ during hatching, and current are controlled at and can guarantee that fish-egg can normally stir, and dissolved oxygen content is controlled at 7.5～9.5 mg/litre during hatching, and brooding time is 150～210 hours;
Six. in the fiberglass pond, continue behind the seed rearing juvenile fish membrane to cultivate to move on in cement pit or the pond after 5～6 days to cultivate; And regularly in cement pit or pond, throw something and feed natural bait or artifical compound feed; But the amino acid absorption ratio in the feed is not so good as natural bait; So preferable methods is the natural bait of throwing something and feeding, the concentration of dissolved oxygen should not be lower than 60% of oxygen saturation in cement pit or the pond.
As a kind of preferred, the body weight of selected raun is more than 5 kilograms in the said step 1, and the body weight of milter is more than 3 kilograms, the breeding density of parent population be the 5-10 tail/square metre.
As a kind of preferred, to cultivate the pond periphery in the said step 2 and avoid the sunshine direct projection, intensity of illumination is controlled at the 0.1-50 lux.
As a kind of preferred, among the said step 3 a, female sturgeon injection sturgeon amount pituitary is a 2-8 mg/kg body weight, and male sturgeon reduces by half; Or in the parent population body, inject carp brain hypophysis, female, male sturgeon injection volume is 4-8 mg/kg body weight.Among the said step 3 b, female sturgeon injection carp amount pituitary is a 3-5 mg/kg body weight, and male sturgeon reduces by half.
As a kind of preferred, in the said step 3, the time interval of hastening parturition with secondary of once hastening parturition is 10-14 hour.
A kind of special-purpose hatching apparatus of artificial fecundation method for Siberian sturgeon comprises feed pipe, water inlet pipe, and distributive pipe also comprises hatching bottle and the collection seedling pipeline that is laid on the hatching bottle base; Feed pipe and water inlet pipe junction are provided with control valve; Hatching bottle upper end is provided with the location lid, and the location lid is used for the location of distributive pipe and water inlet pipe, makes both be positioned at the middle position of hatching bottle; The top of hatching bottle also offers gap, the fry that hatches is flowed out through gap get into collection seedling pipeline.
As a kind of preferred; Said hatching bottle bottom interior is set to a bowl spill, and to prevent there is the current dead angle in the hatching bottle, hatching bottle body is straight bucket column structure; Fish-egg is fully stirred; Obtain fresh current and sufficient oxygen, several hatching bottles are parallel to be laid on the collection seedling ducting side, and the gap on the hatching bottle is towards collection seedling pipeline.
As a kind of improvement; The peripheral equidistance in said distributive pipe lower end be provided with the slide of keeping a foothold, said distributive pipe bottom is higher than the slide bottom of keeping a foothold, the slide bottom of keeping a foothold matches with hatching bottle inside, bottom; The slide of keeping a foothold plays shunting action at the bottom of bottle, at the bottom of bottle, evenly separate current all around.
As a kind of improvement; Said collection seedling pipeline is set to the incline structure that the two ends height does not wait; Collection seedling pipeline is than the storage pond, holding pond that is laid with the seedling that is used for breeding fish temporarily on the low side base, and the centre of storage pond, holding pond is provided with cylindric outlet pipe, and the outlet pipe pipe shaft is provided with the aperture that can supply waste water to discharge; Outlet pipe pipe shaft inwall is placed with guard, can make waste water discharge fry through aperture like this and stay in the storage pond, holding pond.
As a kind of improvement, said hatching bottle is processed by straight bucket column lucite tube, so not only help the hatching process that people observed and managed fish-egg, and manufacture craft is simple, and cost is with low cost.
The present invention is owing to adopt technique scheme; During non-natural propagation, the people is for providing the low temperature stimulation parent population and inject hypophysis, thereby the parent population sexual gland is reached maturity in advance; And in the hatching stage; Gap is offered on top at the hatching bottle, makes the fry that hatches get into collection seedling pipeline, need not the artificial seedling of receiving; Hatching bottle bottom interior is the bowl spill, and body is straight bucket column structure, and fish-egg is fully stirred, and obtains fresh current and sufficient oxygen, has improved incubation rate; On the other hand since the people for the low temperature stimulation parent population being provided and injecting hypophysis; Thereby the parent population sexual gland is reached maturity in advance; Thereby solved the influence that existing Siberia sturgeon seed receives season and region restriction; Thereby can stablize, supply constantly Siberia sturgeon breeding, satisfy the effective demand of raiser the sturgeon breeding.
Description of drawings
Fig. 1 is a hatching apparatus structural representation of the present invention.
Embodiment
Below in conjunction with embodiment the present invention is done further description, a kind of artificial fecundation method for Siberian sturgeon is characterized in that mainly may further comprise the steps:
One selects parent population at winter-spring season, from cultivating pool, fishes for and selects the system stalwartness, and body surface does not have wound, movable normal of the right age female, male parent population; The body weight of raun is more than 5 kilograms, and the body weight of milter is more than 3 kilograms, the breeding density of parent population be controlled at 8 tails/square metre.
Two parent fish rearings
It is that cultivate in 8 ℃ cultivation pond that a puts into water temperature with parent population, and the water temperature that will cultivate after 30 days in the pond is progressively brought up to 16 ℃ by 8 ℃, improves every day about 1 ℃; Water quality is pure and fresh in the maintenance cultivation pond, and oxygen is sufficient, cultivates the pond surrounding enviroment and avoids solar light irradiation, and intensity of illumination is controlled at about 3 luxs;
B changes parent population in the hatchery over to after water temperature rises to 16 ℃, and keeping the water temperature in the hatchery is about 16 ℃, and the hatchery water velocity is controlled at 0.10 meter per second;
Three artificial induced spawnings
A once hastens parturition and in the parent population body, injects the sturgeon hypophysis, and the injection volume of female sturgeon is 6 mg/kg body weight, and male sturgeon reduces by half;
The b secondary is hastened parturition after once hastening parturition 12 hours, in the parent population body, injects the carp hypophysis again, and the injection volume of female sturgeon is 4 mg/kg body weight, and male sturgeon reduces by half;
C puts back to parent population to cultivate in the pond and cultivates behind artificial induced spawning;
Four-player worker fertilization is when estimating the parent population sexual maturity, and ripe essence, ovum are obtained in manual work; Obtain after essence, the ovum with manual method, carry out artificial insemination, fill fertilized egg pouring in the washbasin of clear water then, evenly stir 3～5 minutes after, the water that will contain seminal fluid is outwelled, and cleans several times with clear water;
Five hatchings are put into fry hatching equipment with fertilized egg and are hatched; This all fry hatching equipment comprises feed pipe 2; Water inlet pipe 3, distributive pipe 4 also comprises the hatching bottle of being processed by straight bucket column lucite tube 1; 10 hatching bottle 1 parallel being laid on collection seedling pipeline 5 limits, the gap 8 on the hatching bottle 1 is towards collection seedling pipeline 5.Hatching bottle inside, 1 bottom is the bowl spill, and body is straight bucket column; And be laid in the collection seedling pipeline 5 on hatching bottle 1 base; Hatching bottle 1 upper end is provided with the location and covers 7; The location covers 7 makes distributive pipe 4 and water inlet pipe 3 be positioned at the middle position of hatching bottle; Keep a foothold slide 41 and distributive pipe 4 bottoms of being provided with of the peripheral equidistance in distributive pipe 4 lower ends are higher than slide 41 bottoms of keeping a foothold, and slide 41 bottoms of keeping a foothold match with hatching bottle inside, 1 bottom.The top of hatching bottle 1 also offers gap 8; The fry that hatches is flowed out through gap 8 get into collection seedling pipeline 5; Collection seedling pipeline 5 is set to the incline structure that the two ends height does not wait, and collection seedling pipeline 5 is than being laid with collection on the low side base from the fry of collection seedling pipeline 5 inflows and the storage pond, holding pond 9 of water.The centre of said storage pond, holding pond 9 is provided with cylindric outlet pipe 10, and outlet pipe 10 pipe shafts are provided with the aperture 11 that can supply waste water to discharge, and is placed with guard at outlet pipe 10 pipe shaft inwalls.Fresh current pass through water inlet pipe 3 from feed pipe 2; The current of water inlet pipe evenly separate current through the slide 41 of keeping a foothold that is arranged on distributive pipe 4 bottoms to hatching bottle 1 bottom; Shunting back current up evenly impact fish-egg in the bottom; Fish-egg on making at the bottom of being deposited on bottle constantly up floats, and constantly up rolls in a hatching bottle middle level, makes fish-egg obtain sufficient current and dissolved oxygen; The fry that hatches gets into collection seedling pipeline 5 through gap 8; Fry through action of gravity flow to collection seedling pipeline 5 than getting into storage pond, holding pond 9 behind the low side; Fry is brought up seedling temporarily in storage pond, holding pond 9; Because outlet pipe 10 pipe shaft inwalls are placed with guard, can prevent that fry from flowing out from aperture, waste water is discharged through the aperture 11 that is arranged on outlet pipe 10 pipe shafts.So just realize the effect that fry is gathered automatically, need not artificial the collection, greatly reduced labour intensity; Hatching bottle bottom interior is the bowl spill, and body is straight bucket column structure, and fish-egg is fully stirred, and obtains fresh current and sufficient oxygen; The distributive pipe lower end has been provided with the slide of keeping a foothold of shunting action; Current up evenly impact fish-egg at the bottom of bottle after distributive pipe shunting, the fish-egg on making at the bottom of being deposited on bottle constantly up floats; Constantly roll in the middle level, thereby make fish-egg obtain sufficient fresh current and dissolved oxygen; The hatching bottle is processed by straight bucket column lucite tube; So not only help the hatching process that people observed and managed fish-egg, and hatching bottle manufacture craft is simple, cost is with low cost; The control water temperature is 12 ℃ during hatching; Current are controlled at and can guarantee that fish-egg can normally stir, and dissolved oxygen content is controlled at 8 mg/litre during hatching, and brooding time is about 180 hours;
In the fiberglass pond, continue behind the six seed rearing juvenile fish membranes to cultivate 5～6 days; Transfer to then in cement pit or the pond and cultivate; And regularly in cement pit or pond, throw something and feed natural bait or artifical compound feed, the concentration of dissolved oxygen should not be lower than 60% of oxygen saturation in cement pit or the pond.
The present invention is owing to adopt technique scheme; During non-natural propagation, the people is for providing the low temperature stimulation parent population and inject hypophysis, thereby the parent population sexual gland is reached maturity in advance; And through artificial insemination, hatching and seed rearing; Solved existing Siberia sturgeon seed and received the influence of season and region restriction, thereby can stablize, supply constantly Siberia sturgeon breeding, satisfied the effective demand of raiser the sturgeon breeding.
Claims (4)
1. a Siberia sturgeon artificial propagation hatching apparatus comprises feed pipe (2), water inlet pipe (3), and distributive pipe (4) is characterized in that: also comprise hatching bottle (1) and be laid in the collection seedling pipeline (5) on hatching bottle (1) base; Feed pipe (2) is provided with control valve (6) with water inlet pipe (3) junction; Hatching bottle (1) upper end is provided with location lid (7), and the top of hatching bottle (1) also offers gap (8); The peripheral equidistance in said distributive pipe (4) lower end be provided with the slide of keeping a foothold (41), distributive pipe (4) bottom is higher than the slide of keeping a foothold (41) bottom, the slide of keeping a foothold (41) bottom matches with hatching bottle (1) inside, bottom.
2. a kind of Siberia sturgeon artificial propagation hatching apparatus according to claim 1; It is characterized in that: said hatching bottle (1) bottom interior is the bowl spill; Hatching bottle (1) body is straight bucket column structure; Parallel collection seedling pipeline (5) both sides that are laid in of several hatching bottles (1), gap (8) is towards collection seedling pipeline (5).
3. a kind of Siberia sturgeon artificial propagation hatching apparatus according to claim 1 and 2; It is characterized in that: said collection seedling pipeline (5) is set to the incline structure that the two ends height does not wait; Collection seedling pipeline (5) is than being laid with storage pond, holding pond (9) on the low side base; The centre of said storage pond, holding pond (9) is provided with cylindric outlet pipe (10), and outlet pipe (10) pipe shaft is provided with the aperture (11) that can supply waste water to discharge, and outlet pipe (10) pipe shaft inwall is placed with guard.
4. a kind of Siberia sturgeon artificial propagation hatching apparatus according to claim 1 and 2 is characterized in that: said hatching bottle (1) is processed by straight bucket column lucite tube.
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| CN101569293B (en) * | 2009-05-31 | 2011-08-31 | 中国水产科学研究院东海水产研究所 | Method for inducing gynogenesis of Siberian sturgeon by using amur sturgeon sperm |
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| CN112293315B (en) * | 2020-11-13 | 2022-11-04 | 西藏自治区农牧科学院水产科学研究所 | Large-scale hatching system and method for eggs of Tibetan Lassa nudiflora nojirimus fish |
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| CN2547128Y (en) * | 2002-05-23 | 2003-04-30 | 侯国兴 | Fry incubator |
| CN2648809Y (en) * | 2003-08-27 | 2004-10-20 | 河南省水产科学研究所 | Fish hatcher |
| CN201097554Y (en) * | 2007-08-01 | 2008-08-13 | 杭州千岛湖鲟龙科技开发有限公司 | A kind of fish hatching equipment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101156555A (en) | 2008-04-09 |
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