CN101111762B - 通过双向电泳同时分离生物分子的方法和仪器 - Google Patents

通过双向电泳同时分离生物分子的方法和仪器 Download PDF

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Publication number
CN101111762B
CN101111762B CN2005800405505A CN200580040550A CN101111762B CN 101111762 B CN101111762 B CN 101111762B CN 2005800405505 A CN2005800405505 A CN 2005800405505A CN 200580040550 A CN200580040550 A CN 200580040550A CN 101111762 B CN101111762 B CN 101111762B
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China
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matrix
electrode
biomolecule
electric field
electrophoresis
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Chinese (zh)
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CN101111762A (zh
Inventor
雷纳多·米利奥尼
安东尼奥·瓜达尼诺
马努拉·缪佐
伊莉莎·巴瑞娜
汤玛索·萨拉塔
皮尔乔吉奥·雷迪
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Zip (stock) LLC
Elettrofor S A S Di Ruggero Massimo & C
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Zip (stock) LLC
Elettrofor S A S Di Ruggero Massimo & C
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN2005800405505A 2004-11-26 2005-11-24 通过双向电泳同时分离生物分子的方法和仪器 Expired - Fee Related CN101111762B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IT000301A ITPD20040301A1 (it) 2004-11-26 2004-11-26 Metodo ed apparato per la separazione simultanea di molecole biologiche mediante elettroforesi bidimensionale
ITPD2004A000301 2004-11-26
PCT/IB2005/003519 WO2006056861A1 (en) 2004-11-26 2005-11-24 Method and apparatus for the simultaneous separation of biological molecules by two dimensional electrophoresis

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CN101111762A CN101111762A (zh) 2008-01-23
CN101111762B true CN101111762B (zh) 2011-01-12

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CN2005800405505A Expired - Fee Related CN101111762B (zh) 2004-11-26 2005-11-24 通过双向电泳同时分离生物分子的方法和仪器

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US (1) US20080202934A1 (https=)
EP (1) EP1828760A1 (https=)
JP (1) JP5006790B2 (https=)
KR (1) KR20070089918A (https=)
CN (1) CN101111762B (https=)
CA (1) CA2589232A1 (https=)
IT (1) ITPD20040301A1 (https=)
WO (1) WO2006056861A1 (https=)

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US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
EP3013983B1 (en) 2013-06-25 2023-02-15 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
US10774374B2 (en) 2015-04-10 2020-09-15 Spatial Transcriptomics AB and Illumina, Inc. Spatially distinguished, multiplex nucleic acid analysis of biological specimens
CN105498540A (zh) * 2015-12-08 2016-04-20 北京大学第一医院 一种具有浓缩功能的电泳仪
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
EP3894590B1 (en) 2018-12-10 2025-10-22 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
CN111560063B (zh) * 2020-05-12 2022-11-01 蚌埠医学院 一种动物胰脏来源胰岛素原料药纯化装置及使用方法
CN116134308B (zh) 2020-05-19 2025-05-27 10X基因组学有限公司 电泳盒和仪器
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252747A1 (en) 2020-06-10 2021-12-16 1Ox Genomics, Inc. Fluid delivery methods
EP4491742B1 (en) 2020-09-18 2026-03-04 10X Genomics, Inc. Sample handling apparatus and image registration methods
EP4729631A2 (en) 2020-12-21 2026-04-22 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022178267A2 (en) 2021-02-19 2022-08-25 10X Genomics, Inc. Modular assay support devices
EP4582555A3 (en) 2021-06-03 2025-10-22 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
USD1064308S1 (en) 2021-09-17 2025-02-25 10X Genomics, Inc. Sample handling device
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device

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CN1291913A (zh) * 1998-01-30 2001-04-18 内诺金有限公司 通过双向电泳在一种生物电芯片上无流道分离生物颗粒
US6328870B1 (en) * 2000-01-27 2001-12-11 Cbm Intellectural Properties, Inc. Electrophoresis gel running plate
EP1211510A1 (en) * 2000-11-29 2002-06-05 Proteologics, Inc. Methods and apparatus for nonlinear mobility electrophoresis separation
CN1361720A (zh) * 1999-05-18 2002-07-31 硅生物系统公司 通过双向电泳控制粒子的方法和仪器

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EP0497077B1 (de) * 1991-01-28 1996-07-17 Ciba-Geigy Ag Vorrichtung zur Vorbereitung von Proben insbesondere für Analysezwecke
US6207031B1 (en) * 1997-09-15 2001-03-27 Whitehead Institute For Biomedical Research Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device
ATE359502T1 (de) * 1999-07-16 2007-05-15 Applera Corp Vorrichtung und verfahren für hochdichte elektrophorese
GB0010957D0 (en) * 2000-05-05 2000-06-28 Novartis Ag Compound & method
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Patent Citations (4)

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CN1291913A (zh) * 1998-01-30 2001-04-18 内诺金有限公司 通过双向电泳在一种生物电芯片上无流道分离生物颗粒
CN1361720A (zh) * 1999-05-18 2002-07-31 硅生物系统公司 通过双向电泳控制粒子的方法和仪器
US6328870B1 (en) * 2000-01-27 2001-12-11 Cbm Intellectural Properties, Inc. Electrophoresis gel running plate
EP1211510A1 (en) * 2000-11-29 2002-06-05 Proteologics, Inc. Methods and apparatus for nonlinear mobility electrophoresis separation

Non-Patent Citations (2)

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Garfin D. E..Two-dimensional gel electrophoresis:an overview.TRAC,TRENDS IN ANALYTICAL CHEMISTRY22 5.2003,22(5),263-272.
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Also Published As

Publication number Publication date
EP1828760A1 (en) 2007-09-05
US20080202934A1 (en) 2008-08-28
CN101111762A (zh) 2008-01-23
WO2006056861A1 (en) 2006-06-01
CA2589232A1 (en) 2006-06-01
KR20070089918A (ko) 2007-09-04
JP5006790B2 (ja) 2012-08-22
ITPD20040301A1 (it) 2005-02-26
JP2008522144A (ja) 2008-06-26

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