CN101033467A - 奈瑟球菌基因组序列及其用法 - Google Patents

奈瑟球菌基因组序列及其用法 Download PDF

Info

Publication number
CN101033467A
CN101033467A CNA2007100037383A CN200710003738A CN101033467A CN 101033467 A CN101033467 A CN 101033467A CN A2007100037383 A CNA2007100037383 A CN A2007100037383A CN 200710003738 A CN200710003738 A CN 200710003738A CN 101033467 A CN101033467 A CN 101033467A
Authority
CN
China
Prior art keywords
sequence
appendix
protein
pep
characterized
Prior art date
Application number
CNA2007100037383A
Other languages
English (en)
Inventor
M·皮扎
E·希基
J·彼得森
H·特特林
J·C·文特尔
V·马斯格阿尼
C·加莱奥蒂
M·莫拉
G·拉蒂
M·斯卡塞利
V·斯卡拉托
R·拉普奥利
C·M·弗拉泽
G·格兰迪
Original Assignee
希龙公司
基因组研究所
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US13206899P priority Critical
Priority to US60/132,068 priority
Priority to PCT/US1999/023573 priority patent/WO2000022430A2/en
Priority to USPCT/US99/23573 priority
Priority to GB0004695.3 priority
Priority to GB0004695A priority patent/GB0004695D0/en
Application filed by 希龙公司, 基因组研究所 filed Critical 希龙公司
Priority to PCT/US2000/005928 priority patent/WO2000066791A1/en
Publication of CN101033467A publication Critical patent/CN101033467A/zh

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/22Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Neisseriaceae (F)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/51Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/53DNA (RNA) vaccination
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K38/00Medicinal preparations containing peptides

Abstract

本发明提供了从包括奈瑟球菌的基因组序列获得免疫原性蛋白质的方法,包括脑膜炎奈瑟球菌B的氨基酸序列和对应的核苷酸序列、以及基因组序列。这些获得的蛋白质可用作疫苗、免疫原性组合物和/或诊断的抗原。

Description

奈瑟球菌基因组序列及其用法

本发明专利申请是国家申请号为PCT/US 00/05928、国际申请日为2000年3月8日、进入中国国家阶段的申请号为00809820.4、发明名称为“奈瑟球菌基因组序列及其用法”的发明专利申请的分案申请。

本申请要求以下申请的优先权:美国临时申请No.60/132,068(1999年4月30日提交);PCT/US99/23573(1999年10月8日提交,将于2000年4月出版);和英国专利申请No.GB-0004695.3(2000年2月28日提交)。

本发明涉及从细菌物种:脑膜炎奈瑟球菌(Neisseria meningitidis)获得抗原和免疫原的方法、获得的抗原和免疫原,以及核酸。具体地,涉及从该细菌(更具体地为B血清型)获得的基因组序列。

背景技术

脑膜炎奈瑟球菌(Neisseria meningitidis)是不能动的、对人有致病性的革兰氏阴性双球菌。脑膜炎奈瑟球菌聚居在咽喉处,引起脑膜炎,而且偶尔不引起脑膜炎而引起败血病。脑膜炎奈瑟球菌与淋病奈瑟球菌密切相关,然而脑膜炎球菌与淋球菌明显不同的一个特征是,所有病原性脑膜炎双球菌中存在多糖荚膜。

脑膜炎奈瑟球菌会引起地方性和流行性疾病。在美国,其发病率为每年每100000人有0.6-1人,爆发时可以高得多(见Lieberman等人,(1996)″血清型A/C脑膜炎奈瑟球菌寡糖-蛋白偶联疫苗在幼儿中的安全性和免疫原性″,JAMA 275(19):1499-1503;Schuchat等人(1997)“1995年美国的细菌性脑膜炎”,新英格兰医学杂志(N.Engl.J.Med.)337(14):970-976)。在发展中国家,地方性疾病发病率要高得多,在流行时,发病率可高达每年每100000人500起。该病的死亡率在美国很高,为10-20%,在发展中国家则要高得多。在引入了抗流感嗜血杆菌(haemophilus influenzae)偶联疫苗后,脑膜炎奈瑟球菌是引起美国所有年龄人群中细菌性脑膜炎的主要原因(Schuchat等人(1997)同上)。

根据该菌的荚膜多糖,已经鉴定出12种脑膜炎奈瑟球菌的血清型。A型是亚撒哈拉-非洲地区流行病中最常见的病原体。B型和C型血清型菌是导致美国以及大多数发达国家内大多数病例的原因。W135和Y型血清型菌是导致美国和发达国家的其余病例的原因。目前使用的脑膜炎球菌疫苗是由血清型A、C、Y和W135组成的四价多糖疫苗。尽管其在青年和成人中有效,但是它诱导了弱的免疫应答和短期的保护作用,并且不能用于婴儿[例如,见发病率和死亡率每周报道,46卷,No.RR-5(1997)]。这是因为多糖是T细胞非依赖型抗原,其诱导的弱免疫应答不能通过重复免疫来加强。在流感嗜血杆菌疫苗接种成功后,已经开发出了针对血清型A和C的偶联疫苗,现在是临床测试的最终阶段(Zollinger WD″新的和改进的抗脑膜炎球菌病疫苗″,在:NewGeneration Vaccines中,同上,469-488页;Lieberman等人(1996)同上;Costantino等人(1992)“抗脑膜炎球菌A(menA)和C(menC)的偶联疫苗的开发和I期临床测试”,Vaccine,10:691-698)。

然而,脑膜炎球菌B(menB)仍是一个问题。此血清型目前在美国、欧洲和南美州引起的病例约占总脑膜炎的50%。不能采用多糖方法,因为menB荚膜多糖是α(2-8)-相连的N-乙酰基神经氨酸的聚合物,它也存在于哺乳动物组织中。这导致了对抗原的耐受;实际上,如果引发免疫应答,则该免疫应答是抗自身的,因此是不希望的。为了避免引起自身免疫力并诱导保护性免疫应答,已经对该荚膜多糖进行化学修饰,例如用N-丙酰基代替N-乙酰基,而不改变特异性抗原性(Romero和Outschoorn(1994)″B型脑膜炎球菌候选疫苗的目前状况:荚膜或非荚膜?″Clin Microbiol Rev 7(4):559-575)。

menB疫苗的另一种方法采用外膜蛋白(OMP)的复合混合物,它只含有OMP、或富集在膜孔蛋白中的OMP,或缺失4类OMP(认为它诱导了封闭杀菌活性的抗体)。该方法产生的疫苗的性质还未经完全地分析。它们能保护机体抵抗同源的菌株,但是当存在外膜蛋白的许多抗原性变异株时一般无效。为了克服抗原性变异,已经构建了含有高达9种不同膜孔蛋白的多价疫苗(例如,Poolman JT(1992)“脑膜炎球菌疫苗的发展”Infect.Agents Dis.4:13-28)。用于外膜疫苗的其它蛋白是opa和opc蛋白,但是这些方法均不能克服抗原性变异(例如Ala′Aldeen和Borriello(1996)″脑膜炎球菌运铁蛋白结合蛋白1和2均是外露的,并产生能杀伤同源和异源菌株的杀菌性抗体″Vaccine 14(1):49-53)。

已可得到脑膜炎球菌和淋球菌的基因和蛋白的一定数量的序列信息(例如EP-A-0467714,WO96/29412),但这决不完全。提供进一步的序列,就有机会能鉴定出估计是免疫系统靶标且没有抗原性变异、或者至少比其他更可变区域在抗原性上更保守的、分泌性或外露的蛋白。因此,那些更高度保守的抗原序列是优选的序列。那些对脑膜炎奈瑟球菌或淋病奈瑟球菌特异的且更高度保守的序列是更佳的序列。例如,一些已鉴定的蛋白可作为抗脑膜炎球菌B的有效疫苗成分,一些可作为抗所有脑膜炎球菌血清型的疫苗成分,其它可作为抗所有病原性奈瑟球菌的疫苗成分。从细菌中鉴别出序列还将协助制备生物学探针,尤其是生物体特异性探针。

因此,本发明的一个目的是提供奈瑟球菌的DNA序列,这些DNA序列(1)编码预计和/或已表明具有抗原性或免疫原性的蛋白,(2)用作探针或扩增引物,以及(3)用生物信息学进行分析。

附图简述图1显示了在大肠杆菌中克隆和表达的预期ORF919的蛋白质表达产物和纯化。

图2显示了在大肠杆菌中克隆和表达的预期ORF279的蛋白质表达产物和纯化。

图3显示了在大肠杆菌中克隆和表达的预期ORF576-1的蛋白质表达产物和纯化。

图4显示了在大肠杆菌中克隆和表达的预期ORF519-1的蛋白质表达产物和纯化。

图5显示了在大肠杆菌中克隆和表达的预期ORF121-1的蛋白质表达产物和纯化。

图6显示了在大肠杆菌中克隆和表达的预期ORF128-1的蛋白质表达产物和纯化。

图7显示了在大肠杆菌中克隆和表达的预期ORF206的蛋白质表达产物和纯化。

图8显示了在大肠杆菌中克隆和表达的预期ORF287的蛋白质表达产物和纯化。

图9显示了在大肠杆菌中克隆和表达的预期ORF406的蛋白质表达产物和纯化。

图10显示了在大肠杆菌中克隆和表达的预期ORF919的蛋白质表达产物的亲水性图、抗原指数和AMPHI区域。

图11显示了在大肠杆菌中克隆和表达的预期ORF279的蛋白质表达产物的亲水性图、抗原指数和AMPHI区域。

图12显示了在大肠杆菌中克隆和表达的预期ORF576-1的蛋白质表达产物的亲水性图、抗原指数和AMPHI区域。

图13显示了在大肠杆菌中克隆和表达的预期ORF519-1的蛋白质表达产物的亲水性图、抗原指数和AMPHI区域。

图14显示了在大肠杆菌中克隆和表达的预期ORF121-1的蛋白质表达产物的亲水性图、抗原指数和AMPHI区域。

图15显示了在大肠杆菌中克隆和表达的预期ORF128-1的蛋白质表达产物的亲水性图、抗原指数和AMPHI区域。

图16显示了在大肠杆菌中克隆和表达的预期ORF206的蛋白质表达产物的亲水性图、抗原指数和AMPHI区域。

图17显示了在大肠杆菌中克隆和表达的预期ORF287的蛋白质表达产物的亲水性图、抗原指数和AMPHI区域。

图18显示了在大肠杆菌中克隆和表达的预期ORF406的蛋白质表达产物的亲水性图、抗原指数和AMPHI区域。

本发明脑膜炎奈瑟球菌基因组的第一个完整序列是以961个部分连续核苷酸序列形式公开,如在1999年10月8日提交的、共同拥有的PCT/US99/23573(′573申请)(将在2000年4月出版)中SEQ ID NO:1-961所示。脑膜炎奈瑟球菌的全长基因组的单个序列在′573申请中以SEQ ID NO:1068所示。本发明基于脑膜炎奈瑟球菌的全长基因组,它如本发明附录A中SEQ ID NO:1所示。′573申请的961个序列基本上代表了B血清型脑膜炎奈瑟球菌的全长基因组(>99.98%)。在961个序列中所示961个连续序列(“毗连群”)中某些序列之间有部分重迭。使用TIGR Assembler[G.S.Sutton等人,TIGR Assembler:A New Tool for Assembling Large Shotgun Sequencing Projects,Genome Science andTechnology,1:9-19(1995)],这些重迭区域被用于构建此处附录A中SEQ ID NO:1所示的单个全长序列。毗连群中的某些核苷酸早已被公开(参见万维网(即“WWW”)上的ftp:11ftp.tigr.org/pub/data/n_meningitidis)。在该毗连群中2508个公开序列的坐标在′573申请的附录A中给出。这些数据包括在第1列中给出的毗连群编号(或i.d.);在第2列中的可在WWW上找到的序列名称;以及分别在第3和4列中的坐标。在′573申请的附录B中给出的某些MenB ORF序列,分别是启龙股份公司在1998年10月9日提交的国际专利申请PCT/IB98/01665,在1999年1月14日提交的PCT/IB99/00103中特有的。此处的附录B提供了在此处附录A中SEQ ID NO:1全长序列中所含的2158个开放阅读框的清单。在此处附录B中所列出的信息包括序列的“NMB”名称、推定的翻译产物和在SEQ ID NO:1中包含开放阅读框的起始和终止核苷酸位置。这些开放阅读框在此处被称为“NMB开放阅读框”。

第一方面,本发明提供了核酸,它包括此处附录A中SEQ ID NO:1所示的脑膜炎奈瑟球菌核苷酸序列。还提供了包含与本文公开的核苷酸序列有序列相同性的序列的核酸。根据特定的序列,序列相同性的程度宜大于50%(例如60%、70%、80%、90%、95%、99%或更高)。这些序列包括例如突变体和等位基因变体。本文所述的序列相同性宜用Smith-Watemen同源性搜寻算法在整个序列长度上来确定,例如在MRSRCH程序(Oxford Molecular)中采用仿射缺口搜寻,其中参数“缺口罚分(gap penalty)”为12,“缺口延伸罚分(gap extension penalty)”为1。

本发明还提供了包含本文所列一个或多个核苷酸序列(包括此处附录B所示的NMB开放阅读框)之片段的核酸。这些片段应包含这些序列的至少n个连续的核苷酸,并且根据具体的序列,n为10或更高(例如,11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、30、35、40、45、50、60、75、100或更高)。较佳地,该片段是脑膜炎奈瑟球菌基因组特有的,换言之,该片段并不存在于其他生物体的基因组中。更佳地,该片段是脑膜炎奈瑟球菌B菌株基因组特有的。本发明还提供了与本文提供的核酸杂交的核酸。在本文公开了杂交条件。

本发明还提供了包含与上述那些序列互补的序列的核酸(例如用于反义、探针、或扩增引物目的)。

当然,本发明的核酸可用许多方式制得(例如化学合成,从DNA文库、或从生物体本身制得等),并可采用各种形式(例如单链、双链、载体、探针、引物等)。术语“核酸”包括DNA和RNA,以及它们的类似物,如含有修饰骨架的那些类似物,还包括肽核酸(PNA)等。

应理解,因为′573申请中SEQ ID NO:1-961代表了该生物体的基本上完整的基因组并有部分重迭,因此,对′573申请中SEQ ID NO:1-961的引用也包括了在其范围内的、对完整基因组序列即SEQ ID NO:1的引用。例如,当两个SEQ ID NO重迭时,本发明包括通过拼接两个重迭序列所形成的、其全长序列可在SEQ ID NO:1中找到的单一序列。因此,例如,一个横跨2个SEQ ID NO但其本身并不完整地列于任一SEQ IDNO的核苷酸序列,仍然在本发明范围之内。这样的序列会完整地存在于本申请SEQ IDNO:1的单一全长序列中。

本发明还提供了含有本发明的核苷酸序列的载体(如表达载体、测序载体、克隆载体等)以及用这些载体转化的宿主细胞。

另一方面,本发明提供了一些蛋白,这些蛋白含有本文公开的脑膜炎奈瑟球菌核苷酸序列所编码的氨基酸序列。还提供了含有与这些蛋白质有序列相同性的序列的蛋白。根据具体的序列,序列相同性程度宜大于50%(例如60%、70%、80%、90%、95%、99%或更高)。序列相同性可用上面公开的方法确定。这些同源蛋白包括在本文所给出的脑膜炎奈瑟球菌核苷酸序列所编码的突变体和等位基因变异体。

本发明还提供了包含序列表所示的脑膜炎奈瑟球菌核苷酸序列所编码的氨基酸序列片段的蛋白。这些片段应包含诸序列中至少n个连续的氨基酸,并且根据具体的序列,n为7或更高(例如,8、10、12、14、16、18、20或更高)。各片段宜包含该序列的一个表位。

本发明的蛋白当然可用各种方法(例如重组表达、从细胞培养中纯化、化学合成等)制成各种形式(例如天然的、融合蛋白等)。它们宜制成基本上分离的形式(即基本上不含其它脑膜炎奈瑟球菌宿主细胞蛋白)。

可用各种不同测试来评估本发明蛋白的体内免疫原性。例如,蛋白质可重组表达或化学合成,然后通过免疫印迹用于筛选病人血清。蛋白和病人血清之间的阳性反应就表示,该病人早已产生针对有关蛋白的免疫应答,即该蛋白质是免疫原。该方法还可用于鉴别免疫优势蛋白。

本发明还提供了编码本发明蛋白的核酸。

另一方面,本发明提供了含有本发明核酸的核苷酸序列的计算机、计算机存储器、计算机存储介质(如软盘、硬盘、CD-ROM等)和/或计算机数据库。较佳地,它含有本文列出的一种或多种脑膜炎奈瑟球菌核苷酸序列。

这可用于分析本文所列出脑膜炎奈瑟球菌核苷酸序列。例如,可用于检索以鉴别序列中的开放阅读框(ORF)或编码序列。

另一方面,本发明提供了鉴别氨基酸序列的方法,它包括步骤:检索本文所列出的脑膜炎奈瑟球菌核苷酸序列中推定的开放阅读框或蛋白编码序列。类似地,本发明提供了此处所列出的脑膜炎奈瑟球菌核苷酸序列在检索推定的开放阅读框或蛋白质编码序列中的用途。

开放阅读框或蛋白编码序列分析通常在计算机上用标准的生物信息技术进行。用于分析的典型算法或程序包括:ORFFINDER(NCBI),GENMARK[Borodovsky &McIninch(1993)Computers Chem 17:122-133]和GLIMMER[Salzberg et al.(1998)NuclAcids Res 26:544-548]。

对开放阅读框或蛋白编码序列的检索可包括步骤:检索此处所列出的脑膜炎奈瑟球菌核苷酸序列中的起始密码子和检索上游序列中的处于读框中终止密码子。中间的密码子就代表了推定的蛋白编码序列。通常,应检索一个序列中6种可能的读框。

用这种方法鉴别出的氨基酸序列可用任何合适的系统进行表达,以获得蛋白。该蛋白可用于产生抗体,而该抗体识别在鉴别出的氨基酸序列中的表位。这些抗体可用于筛选脑膜炎奈瑟球菌,以检测是否存在含所鉴别氨基酸序列的蛋白质。

此外,一旦鉴别出ORF或蛋白编码序列,那么就可将该序列与序列数据库进行比较。序列分析工具可在NCBI(http://www.ncbi.nlm.nih.gov)处找到,例如BLAST,BLAST2,BLASTn,BLASTp,tBLASTn,BLASTx,和tBLASTx算法[还可参见Altschul等人.(1997)Gapped BLAST and PSI-BLAST:new generation of protein database search programs.Nucleic Acids Research 25:2289-3402]。用于比较的合适数据库包括非冗余GenBank,EMBL,DDBJ和PDB序列,以及非冗余GenBank CDS翻译物,PDB,SwissProt,Spupdate和PIR序列。这种比较可给出蛋白质功能的指示。

氨基酸序列中的疏水区可用以上算法加以预测,例如基于Esposti等人的统计学研究的算法[″膜蛋白亲水性的关键评价″(Critical evaluation of the hydropathy of membraneproteins)(1990)Eur J Biochem 190:207-219]。疏水区代表了潜在的跨膜区或疏水性前导序列,这暗示蛋白质可以被分泌或位于表面。这些特性通常是良好免疫原的标志。

类似地,可用PSORT算法(http://www.psort.nibb.ac.jp)来预测跨膜区或前导序列,以及用MOTIFS程序来预测功能区(GCG Wisconsin & PROSITE)。

本发明还提供了一种核酸,该核酸含有本文所列出的脑膜炎奈瑟球菌核苷酸序列中的开放阅读框或蛋白编码序列。此外,本发明还提供了一种蛋白质,该蛋白质含有所述开放阅读框或蛋白编码序列所编码的氨基酸序列。

另一方面,本发明提供了结合这些蛋白的抗体。它们可能是多克隆的或单克隆的,可用本领域技术人员已知的任何合适方法制得。

本发明的抗体可以以各种不同方式使用,例如用于证实蛋白质的表达,或用于证实蛋白质表达的场所。例如,标记的抗体(如荧光标记以用于FACS(荧光激活细胞分选仪))可以与完整的细菌一起孵育,而在细菌表面上存在标记就证实蛋白质的位置。

另一方面,本发明提供了含有本发明蛋白质、抗体和/或核酸的组合物。这些组合物适合作为疫苗、免疫原性组合物或诊断试剂。

本发明还提供了本发明的核酸、蛋白或抗体用作药剂(例如作为疫苗)或作为诊断性试剂的用途。本发明还提供了本发明的核酸、蛋白或抗体在生产下列物质中的应用:(i)用于治疗或预防奈瑟球菌感染的药剂;(ii)用于检测奈瑟球菌或检测抗奈瑟球菌抗体是否存在的诊断性试剂。所述奈瑟球菌可以是任何菌种或菌株(例如淋病奈瑟球菌),但优选地是脑膜炎奈瑟球菌,尤其是脑膜炎奈瑟球菌菌株A、菌株B或菌株C。

另一方面,本发明还提供了含有蛋白质、核酸分子、或抗体的组合物。本发明的较优选方面是蛋白质的免疫原性组合物。本发明的更优选方面包括蛋白质的药物免疫原性组合物或疫苗,以及其在制造用于治疗或预防奈瑟球菌感染(尤其是MenB感染)的药物方面的用途。

本发明还提供了一种治疗患者的方法,该方法包括给予患者治疗有效量的本发明的核酸、蛋白和/或抗体。

另一方面,本发明提供了各种方法。

提供了一种生产本发明的蛋白的方法,该方法包括步骤:在诱导蛋白表达的条件下,培育本发明的宿主细胞。一种方法还可包括化学合成蛋白或化学合成(至少部分合成)核苷酸。

提供了一种检测本发明的多核苷酸的方法,该方法包括下列步骤:(a)在杂交条件下使本发明的核酸探针与生物样品接触,形成双链体;和(b)检测所述双链体。

提供了一种检测本发明的蛋白质的方法,该方法包括下列步骤:(a)在适合形成抗体-抗原复合物的条件下使本发明的抗体和生物样品接触;和(b)检测所述复合物。

另一方面,本发明提供了检测选择性结合于抗原或多肽或蛋白质的抗体的方法,这些抗原或多肽或蛋白质是对奈瑟球菌的任何菌种或菌株特异性的,较佳地是对淋病奈瑟球菌菌株特异性的,但更佳地是对脑膜炎奈瑟球菌(尤其是菌株A、菌株B或菌株C,特别是MenB)特异的。该方法包括步骤:(a)在适合形成抗体-抗原复合物的条件下,使本发明抗原或多肽或蛋白质与生物样品接触;和(b)检测所述复合物。

在现已概述了本发明之后,通过参考下列实施例可以更容易地理解本发明。下列实施例是以阐述方式提供的,并不意味限制本发明,除非指明。

方法学-标准程序和技术的概述综述本发明提供了脑膜炎奈瑟球菌menB核苷酸序列、和其所编码的氨基酸序列。利用这些所公开的序列,可以产生核酸探针试验和表达盒及载体。蛋白质还可化学合成。表达载体可以转入宿主细胞以产生蛋白。纯化或分离的多肽可用于产生抗体以检测menB蛋白。而且,宿主细胞或提取物可用于生物试验以分离促效剂和拮抗剂。此外,利用这些序列,人们可检索以鉴定开放读框(ORF)和鉴定氨基酸序列。蛋白质还可用于免疫原性组合物以及用作疫苗组份。

除非另有描述,本发明的实施将采用分子生物学、微生物学、重组DNA和免疫学的常规技术,这些均是本领域技术人员所知的。这些技术在下列文献中有全面的描述:例如,Sambrook《分子克隆实验手册》第2版(1989);《DNA克隆》第I和II卷(D.N.Glover编1985);《寡核苷酸合成》(M.J.Gait编,1984);《核酸杂交》(B.D.Hames和S.J.Higgins编.1984);《转录和翻译》(B.D.Hames和S.J.Higgins编,1984);《动物细胞培养》(R.I.Freshney编,1986);《固定化细胞和酶》(IRL出版社,1986);B.Perbal,《分子克隆实用指南》(1984);《酶学方法》系列丛书(Academic Press,Inc.),尤其是154和155卷;《哺乳动物细胞的基因转移载体》(J.H.Miller和M.P.Calos编,1987,Cold SpringHarbor Laboratory);Mayer和Walker编(1987),《细胞和分子生物学的免疫化学方法》(Academic Press,London);Scopes,(1987)《蛋白质纯化:原理和实践》第2版(Springer-Verlag,N.Y.),以及《实验免疫学手册》I-IV卷(D.C.Weir和C.C.Blackwell编1986)。

在本说明书中采用了核苷酸和氨基酸的标准缩写。

本文引用的所有出版物、专利和专利申请均全部纳入本文作参考。

表达系统奈瑟球菌menB核苷酸序列可在各种不同的表达系统中表达;例如与哺乳动物细胞、植物细胞、杆状病毒、细菌和酵母一起使用的那些系统。

i.哺乳动物系统哺乳动物表达系统是本领域中已知的。哺乳动物启动子是能结合哺乳动物RNA聚合酶并启动下游(3′)编码序列(如结构基因)转录成mRNA的任何DNA序列。启动子具有一个转录起始区,其通常邻近编码序列的5′端,还具有一个TATA盒,其通常位于转录起始位点上游25-30个碱基对(bp)处。认为TATA盒指导RNA聚合酶II在正确位点开始RNA合成。哺乳动物启动子还含有一个上游启动子元件,其通常位于TATA盒上游100至200bp内。该上游启动子元件决定了转录启动的速度,并可在两个方向之一上起作用[Sambrook等人(1989)“克隆基因在哺乳动物细胞中的表达”《分子克隆:实验手册》,第2版]。

哺乳动物病毒基因通常是高表达的,具有宽的宿主范围;因此,编码哺乳动物病毒基因的序列提供了特别有用的启动子序列。例子包括SV40早期启动子、小鼠乳房肿瘤病毒LTR启动子、腺病毒主要晚期启动子(Ad MLP)以及单纯疱疹病毒启动子。另外,从非病毒基因(如鼠金属硫蛋白基因)衍生的序列也提供了有用的启动子序列。表达可以是组成型的或受调控的(诱导型)。根据所选用的启动子,许多启动子可用已知的底物进行诱导,例如使用带糖皮质素反应元件(GRE)的小鼠乳腺瘤病毒(MMTV)启动子,该启动子可在激素反应性转化细胞中用促糖皮质激素加以诱导(参见例如美国专利5,783,681)。

增强元件(增强子)的存在,联合上述启动子元件通常会提高表达水平。增强子是这样一种调控性DNA序列,当其与同源或异源启动子相连,合成在正常的RNA起始位点开始时,它能刺激转录提高1000倍。当增强子位于转录起始位点的上游或下游,处于正常或翻转方向,或距离启动子1000个核苷酸以上的距离时,它均具有活性[Maniatis等人(1987)Science 236:1237;Alberts等人(1989)《细胞分子生物学》,第2版]。从病毒衍生获得的增强子元件可能是特别有用的,因为它们通常具有较宽的宿主范围。例子包括SV40早期基因增强子[Dijkema等人(1985)EMBO J.4:761]以及衍生自Rous肉瘤病毒的长末端重复序列(LTR)的增强子/启动子[Gorman等人(1982b)Proc.Natl.Acad.Sci.79:6777]以及来自人巨细胞病毒的增强子/启动子[Boshart等人(1985)Cell 41:521]。另外,一些增强子仅仅在诱导物(例如激素或金属离子)的存在下是可调节的并具有活性[Sassone-Corsi和Borelli(1986)Trends Genet.2:215;Maniatis等人(1987)Science 236:1237]。

DNA分子可在哺乳动物细胞中胞内表达。启动子序列可以和DNA分子直接相连,在这种情况下,重组蛋白的N端第一个氨基酸始终是甲硫氨酸,其由ATG起始密码子编码。如果需要,可通过和溴化氰体外培育来从蛋白上切下此N端。

另外,外来蛋白也可从细胞中分泌到生长培养基中,方法是产生嵌合的DNA分子,该DNA分子编码的融合蛋白包括一前导序列片段,该片段在哺乳动物细胞中提供了外源蛋白的分泌。较佳的,在前导序列片段和外源基因之间可以有能在体内或体外断裂的加工位点。前导序列片段通常编码一种信号肽,该信号肽由引导蛋白分泌出细胞的疏水性氨基酸组成。腺病毒三联前导序列是哺乳动物细胞中分泌外来蛋白的一个前导序列的例子。

通常,哺乳动物细胞识别的转录终止和聚腺苷酸化序列是位于翻译终止密码子3′的调控区域,因此它和启动子元件一起连接在编码序列的侧面。成熟mRNA的3′端通过定点转录后断裂和聚腺苷酸化而形成[Birnstiel等人(1985)Cell 41:349;Proudfoot和Whitelaw(1988)″真核RNA的终端和3′端加工″《转录和剪接》(B.D.Hames和D.M.Glover编);Proudfoot(1989)Trends Biochem.Sci.14:105]。这些序列指导mRNA的转录,mRNA能被翻译成该DNA编码的多肽。转录终止子/聚腺苷酸化信号的例子包括从SV40衍生的那些[Sambrook等人(1989)“克隆基因在培养的哺乳动物细胞中的表达”《分子克隆实验手册》]。

通常,上述组件,包括启动子、聚腺苷酸化信号以及转录终止序列被一起放在表达构建物中。如果需要,表达构建物中还包括增强子、具有功能性剪接供体和受体位点的内含子以及前导序列。表达构建物通常以复制子形式维持,例如是能在宿主(如哺乳动物细胞或细菌)中稳定维持的染色体外元件(如质粒)。哺乳动物复制系统包括从动物病毒衍生的那些系统,其需要反式作用因子来进行复制。例如,含有乳多空病毒或多瘤病毒复制系统的质粒,如SV40[Gluzman(1981)Cell 23:175],在合适的病毒T抗原存在下复制出极高的拷贝数。哺乳动物复制子的其它例子包括衍生自牛乳头瘤病毒和EB病毒的复制子。另外,复制子可以有两个复制系统,从而使其能维持在例如哺乳动物细胞中进行表达并能在原核宿主中克隆和扩增。这些哺乳动物细菌穿梭载体的例子包括pMT2[Kaufman等人(1989)Mol.Cell.Biol.9:946]和pHEBO[Shimizu等人(1986)Mol.Cell.Biol.6:1074]。

所用的转化程序取决于待转化的宿主。将异源多核苷酸导入哺乳动物细胞中的方法是本领域所知的,其包括葡聚糖介导的转染、磷酸钙沉淀、Polybrene(1,5-二甲基-1,5-二氮十一亚甲基聚甲溴化物)介导的转染、原生质体融合、电穿孔、将多核苷酸包裹在脂质体中以及将DNA直接显微注射到胞核中。

可作为宿主进行表达的哺乳动物细胞系是本领域中已知的,其包括许多从美国典型培养物保藏中心(ATCC)获得的无限增殖细胞系,包括但不局限于,中国仓鼠卵巢(CHO)细胞、海拉细胞、乳仓鼠肾(BHK)细胞、猴肾细胞(COS)、人肝细胞癌细胞(如HepG2)和其它许多细胞系。

ii.植物细胞表达系统本领域已知有许多植物细胞培养系统和全植物遗传表达系统。典型的植物细胞基因表达系统包括在以下专利中描述的那些,例如:US 5,693,506;US 5,659,122;和US5,608,143。Zenk,Phytochemistry 30:3861-3863(1991)中描述了在植物细胞培养物中遗传表达的其它例子。除上述参考文献外,关于植物蛋白信号肽的描述还可在下列文献中找到:Vaulcombe等人,Mol.Gen.Genet.209:33-40(1987);Chandler等人,Plant MolecularBiology 3:407-418(1984);Rogers,J.Biol.Chem.260:3731-3738(1985);Rothstein等人,Gene 55:353-356(1987);Whittier等人,Nucleic Acids Research 15:2515-2535(1987);Wirsel等人,Molecular Microbiology 3:3-14(1989);Yu等人,Gene 122:247-253(1992)。关于用植物激素、赤霉素酸和赤霉素酸诱导分泌的酶调节植物基因表达的描述可在R.L.Jones和J.MacMillin,Gibberellins,《植物生理学进展》,Malcolm B.Wilkins编辑,1984 Pitman Publishing Limited,London,21-52页中找到。描述其它调节代谢的基因的参考文献参见:Sheen,Plant Cell,2:1027-1038(1990);Maas等人,欧洲分子生物学协会杂志(EMBO J.)9:3447-3452(1990);Benkel和Hickey,美国科学院院报(Proc.Natl.Acad.Sci.).84:1337-1339(1987)。

通常,利用本领域已知的技术,将所需的多核苷酸序列插入一表达盒中,该表达盒含有为在植物中操作而设计的基因调控元件。将该表达盒插入所需的表达载体中,表达盒的上游和下游有适合在植物宿主中表达的伴随序列。这些伴随序列可来自质粒或病毒,并为载体提供所需的性能,以允许载体将DNA从起初的克隆宿主(如细菌)中移动到所需植物宿主中。基础的细菌/植物载体构建物最好能提供宽的宿主范围原核复制起点;原核可选择标记;以及,对于农杆菌转化而言,宜提供T DNA序列用于农杆菌介导的转移至植物染色体。当异源基因不易检测时,该构建物最好还具有一个适用于确定植物细胞是否已经转化的可选择标记基因。关于合适标记(例如对于禾草类家族成员)的综述可在Wilmink和Dons,1993,Plant Mol.Biol.Reptr,11(2):165-185中找到。

还建议采用适合将异源序列整合到植物基因组中的序列。这些序列可能包括用于同源重组的转座子序列以及允许将异源表达盒随机插入植物基因组中的Ti序列。合适的原核可选择标记包括抗生素(如氨苄青霉素或四环素)抗性标记。编码其它功能的其它DNA序列也可存在于载体中,这是本领域所知的。

本发明的核酸分子可包括在一个表达盒中来表达感兴趣的蛋白质。通常只要一个表达盒,但是两个或多个表达盒也是可行的。除了编码异源蛋白的序列外,重组表达盒还含有下列元件:启动子区域、植物5′非翻译序列、起始密码子(根据结构基因原来是否具有而定)、以及转录和翻译终止序列。表达盒5′和3′端的独特限制性酶位点能使表达盒方便地插入预先存在的载体中。

异源编码序列可以是任何与本发明有关的蛋白。编码感兴趣的蛋白的序列将编码出一个信号肽,该信号肽能适当地加工和转运蛋白质,并且通常缺少可能会导致本发明的所需蛋白与膜结合的序列。对于大部分来说,由于转录起始区是发芽期所表达和转运的基因的转录起始区,采用提供转运的信号肽,也可提供转运感兴趣的蛋白质。通过这种方式,感兴趣的蛋白将从表达该蛋白的细胞中转运出来,并能被有效地收获。通常,种子中的分泌是通过糊粉或小盾体上皮层进入种子的胚乳。尽管不需要使蛋白从产生该蛋白的细胞中分泌出来,但是这种分泌有利于重组蛋白的分离和纯化。

由于所需基因产物的最终表达将在真核细胞中进行,因此需要确定克隆的基因部分是否含有作为内含子被宿主剪接体机制加工的序列。如果是这样,需要对“内含子”区进行定点诱变,以防止一部分遗传信息作为错误的内含子密码而丧失,Reed和Maniatis,Cell 41:95-105,1985。

可用微量移液管以机械方式转移重组DNA,将载体直接显微注射到植物细胞中。Crossway,Mol.Gen.Genet,202:179-185。还可用聚乙二醇将遗传物质转移到植物细胞中,Krens等人,Nature,296,72-74,1982。导入核酸片段的另一种方法是用小颗粒进行高速弹道贯穿,在这些小珠或颗粒的基质中或表面上带有核酸,Klein等人,Nature,327,70-73,1987,Knudsen和Muller,1991,Planta,185:330-336提出用颗粒轰击大麦胚乳以产生转基因大麦。还有一种导入方法是使原生质体和其它实体(微细胞(minicell)、细胞、溶酶体或其它可融合的脂质表面体)融合,Fraley等人,美国科学院院报(Proc.Natl.Acad.Sci.USA),79,1859-1863,1982。

载体也可通过电穿孔导入植物细胞中。(Fromm等人,PNAS 82:5824,1985)。在该技术中,在含有基因构建物的质粒存在下电穿孔植物原生质体。高电场强度的电脉冲使生物膜可逆地被通透,从而允许导入质粒。电穿孔的植物原生质体重新形成细胞壁,分裂并形成植物愈伤组织。

能分离出原生质体并能培育成全再生植物的所有植物,都能用本发明进行转化,从而回收得到含有转基因的全植物。已经知道实际上可以从培育的细胞或组织再生所有的植物,其包括但不局限于,甘蔗、甜菜、棉花、水果和其它树、豆科植物和蔬菜的所有主要种类。一些合适的植物包括,例如,草莓属、莲花属、苜蓿属、驴食豆属、三叶草属、胡卢巴属、豇豆属、柑橘属、亚麻属、老鹳草属、木薯属(Manihot)、胡萝卜属(Daucus)、鼠耳芥属、芸苔属、萝卜属、白芥属、颠茄属、辣椒属、曼陀罗属、天仙子属、番茄属、烟草属、茄属、碧冬茄属、毛地黄属、Majorana、菊苣属、向日葵属、莴苣属、雀麦属、天门冬属、金鱼草属、龙骨角属、龙面花属(Nemesia)、天竺葵属、稷属、狼尾草属、毛茛属、千里光属、Salpiglossis、香瓜属、Browaalia、大豆属、黑麦草属、玉蜀黍属、小麦、蜀黍属和曼陀罗属各种类。

再生方式随各种植物种类而有所不同,但是通常是首先提供含有异源基因拷贝的转化的原生质体悬液。形成愈伤组织,从愈伤组织中诱生出枝条,随后是根。另外,从原生质体悬液可以诱生形成胚胎。这些胚胎象天然的胚胎那样发芽形成植物。培养基通常含有各种氨基酸和激素,如植物生长素和细胞分裂素。尤其是对于玉米和苜蓿属来说,在培养基中加入谷氨酸和脯氨酸也是很有利的。枝条和根通常同时发育。有效的再生取决于培养基、基因型以及培养史。如果控制了这三个变量,那么再生能完全再现和重复。

在一些植物细胞培养系统中,本发明所需的蛋白可能被排泄出来,或者蛋白可从全植物中提取出来。当本发明所需的蛋白被分泌到培养基中后,就可进行收集。或者,可以用机械方式破碎胚以及无胚-半种子或其它植物组织,以释放出分泌到细胞和组织之间的蛋白。将该混合物悬于缓冲液中,以收回可溶性蛋白。然后用常规的蛋白分离和纯化方法纯化重组蛋白。用常规方法调节时间、温度、pH、氧和体积等参数,以优化异源蛋白的表达和回收。

iii.杆状病毒系统也可将编码蛋白质的多核苷酸插入合适的昆虫表达载体中,并与该载体中的控制元件操作性相连。载体构建采用本领域已知的技术。总地来说,表达系统的组分包括一种转移载体,通常是细菌质粒,其含有杆状病毒基因组片段以及便于插入待表达异源基因的常规限制性位点;野生型杆状病毒,其序列与转移载体中的杆状病毒特异性片段同源(这使得异源基因能同源重组到杆状病毒基因组中);以及合适的昆虫宿主细胞和生长培养基。

将编码蛋白质的DNA序列插入转移载体中后,将载体和野生型病毒基因组转染到昆虫宿主细胞中,使载体和病毒基因组重组。表达包装的重组病毒,鉴定并纯化重组噬斑。杆状病毒/昆虫细胞表达系统材料及其方法,除别的以外,可以试剂盒形式购自Invitrogen,San Diego CA(″MaxBac″试剂盒)。这些技术通常是本领域技术人员所知的,在Summers和Smith的Texas Agricultural Experiment Station Bulletin No.1555(1987)(后称“Summer和Smith的文章”)中有充分描述。

在将编码蛋白质的DNA序列插入杆状病毒基因组之前,通常将上述组件,包括启动子、前导序列(如果需要)、感兴趣的编码序列以及转录终止序列装配在中间置换型构建物(转移载体)中。该构建物可含有单个基因以及操作性相连的调控元件;多个基因,每个基因有其自己的一套操作性相连调控元件;或是由同一组调控元件调控的多个基因。中间置换型构建物通常保持在一个复制子中,例如能在宿主(如细菌)内稳定保持的染色体外元件(如质粒)。复制子将具有一个复制系统,从而使其能保持在合适的宿主中进行克隆和扩增。

目前,用来将外源基因导入AcNPV的最常用的转移载体是pAc373。还可设计本领域技术人员已知的其它许多载体。这些载体例如包括,pVL985(其将多角体蛋白的起始密码子从ATG变为ATT,在ATT下游32个碱基对处引入一个BamHI克隆位点;见Luckow和Summers,Virology(1989)17:31)。

质粒通常还含有多角体蛋白聚腺苷酸化信号(Miller等人(1988)Ann.Rev.Microbiol.,42:177)以及用来在大肠杆菌中选择和繁殖的原核氨苄青霉素抗性(amp)基因和复制起点。

杆状病毒转移载体通常含有杆状病毒启动子。杆状病毒启动子是能结合杆状病毒RNA聚合酶并启动下游(5′到3′)编码序列(如结构基因)转录成mRNA的DNA序列。启动子具有一个转录起始区,该区通常邻近编码序列的5′端。该转录起始区通常包括一个RNA聚合酶结合位点以及一个转录起始位点。杆状病毒转移载体还可能具有称为增强子的第二区,如果该区域存在,它通常远离结构基因。表达可以是调控型或组成型的。

在病毒感染周期晚期大量转录的结构基因提供了特别有用的启动子序列。例子包括从编码病毒多角体蛋白的基因衍生获得的序列,Friesen等人(1986)“杆状病毒基因表达的调控”《杆状病毒分子生物学》(Walter Doerfler编辑);EPO公开号127 839和155476;以及编码p10蛋白的基因,Vlak等人(1988),J.Gen.Virol.69:765。

编码合适的信号序列的DNA可以衍生自分泌的昆虫或杆状病毒蛋白(如杆状病毒多角体蛋白基因)的基因(Carbonell等人,(1988)Gene,73:409)。另外,由于哺乳动物细胞翻译后修饰的信号(如信号肽断裂、蛋白水解断裂和磷酸化)看来可被昆虫细胞识别,且分泌和胞核积累所需的信号看来在非脊椎动物细胞和脊椎动物细胞之间是保守的,因此也可用非昆虫来源的前导序列来提供昆虫中的分泌,这些前导序列例如是从编码人α-干扰素(Maeda等人(1985),Nature 315:592)、人胃泌素释放肽(Lebacq-Verheyden等人(1988),Molec.Cell.Biol.8:3129)、人IL-2(Smith等人(1985)PNAS,82:8404)、小鼠IL-3(Miyajima等人(1987)Gene 58:273)和人葡糖脑苷脂酶(Martin等人(1988)DNA,7:99)的基因衍生获得的。

重组多肽或聚蛋白可以在胞内表达,或如果用合适的调控序列表达,它可被分泌。非融合的外源蛋白的良好胞内表达通常需要理想的具有短前导序列的异源基因在ATG起始信号前有合适的翻译起始信号。如果需要,可通过和溴化氰体外培育来从成熟蛋白上切下N端甲硫氨酸。

另外,可通过产生嵌合的DNA分子将非天然分泌的重组聚蛋白或蛋白从昆虫细胞中分泌出来,该嵌合的DNA分子所编码的融合蛋白包含一前导序列片段,该片段提供了昆虫中分泌外源蛋白的作用。该前导序列片段通常编码一种信号肽,该信号肽包含的疏水性氨基酸引导蛋白质转移到内质网中。

在插入了编码该蛋白表达产物前体的DNA序列和/或基因后,用转移载体的异源DNA和野生型杆状病毒的基因组DNA共同转化(通常是共转染)昆虫细胞宿主。构建物的启动子和转录终止序列通常包含2-5kb的杆状病毒基因组片段。将异源DNA引入杆状病毒中所需位点内的方法是本领域所知的。(见Summers和Smith的文章,同上;Ju等人(1987);Smith等人,Mol.Cell.Biol.(1983)3:2156;和Luckow和Summers(1989))。例如,插入可以是通过同源双交换重组来插入一个基因如多角体蛋白基因中;插入还可以是插入经工程改造而编入所需杆状病毒基因内的限制性酶切位点中。Miller等人(1989),Bioessays 4:91。当DNA序列被克隆在表达载体多角体蛋白基因位置中时,其5′和3′均侧接了多角体蛋白特异性序列,并位于多角体蛋白启动子的下游。

随后将新形成的杆状病毒表达载体包装到感染性重组杆状病毒中。发生同源重组的频率很低(在约1%和5%之间);因此,共转染后产生的大多数病毒仍是野生型病毒。因此,需要用一种方法来鉴别重组病毒。该表达系统的一个优点是肉眼筛选能区分重组病毒。在病毒感染后期,天然病毒产生的多角体蛋白在受其感染细胞的胞核中产生的水平非常高。累积的多角体蛋白形成的包涵体还含有包埋颗粒。这些包涵体的大小为15微米,它们具有高度的折光性,从而使它们呈现明亮的发光外观,在光学显微镜下很容易观察。感染了重组病毒的细胞缺少包涵体。为了区分重组病毒和野生型病毒,用本领域已知的技术将转染上清接种到单层昆虫细胞上形成噬斑。即,在光学显微镜下筛选存在(表明是野生型病毒)或不存在(表明是重组病毒)包涵体的噬斑。“当代微生物学方法”第2卷(Ausubel等人编辑),16.8(增补10,1990);Summers和Smith,同上;Miller等人(1989)。

已经开发出感染进入几种昆虫细胞的重组杆状病毒表达载体。例如,已经开发出用于感染以下昆虫细胞的重组杆状病毒:埃及伊蚊、苜蓿丫纹夜蛾、家蚕、黑尾果蝇、草地夜蛾和粉纹夜蛾(PCT出版物WO 89/046699;Carbonell等人(1985)J.Virol.56:153;Wright(1986)Nature 321:718;Smith等人(1983)Mol.Cell.Biol.3:2156;综述见Fraser等人(1989)In Vitro Cell.Dev.Biol.25:225)。

可以购得细胞和细胞培养基用于在杆状病毒/表达系统中直接表达和融合表达异源多肽;细胞培养技术是本领域技术人员通常所知的。例如见Summers和Smith,同上。

然后,经修饰的昆虫细胞可以生长在合适的营养培养基中,该培养基能稳定地保持该质粒于修饰的昆虫宿主中。当表达产物的基因处于可诱导的控制下时,可以使宿主生长至高密度,并诱导表达。另外,当表达是组成型表达时,产物将被连续表达到培养基中,营养性培养基必需不断循环,取出感兴趣的产物同时补充消耗的营养物。产物可用以下这些技术来纯化:例如层析,如HPLC、亲和层析、离子交换层析等;电泳;密度梯度离心;溶剂抽提等。产物可按需作进一步纯化,以基本上除去所有也分泌到培养基中或由昆虫细胞裂解而产生的昆虫蛋白,以提供一种至少基本上不含宿主碎片如蛋白质、脂质和多糖的产物。

为了进行蛋白质表达,将从转化子衍生获得的重组宿主细胞培育在允许重组蛋白的编码序列表达的条件下。这些条件将随所选定的宿主细胞而变。然而,本领域技术人员容易根据本领域已知的知识来确定该条件。

iv.细菌系统细菌表达技术是本领域已知的。细菌启动子是能结合细菌RNA聚合酶并启动下游(3′)编码序列(如结构基因)转录成mRNA的DNA序列。启动子具有一个转录起始区,其通常位于编码序列的5′端附近。该转录起始区通常包括RNA聚合酶结合位点以及一个转录起始位点。细菌启动子可能还有第二个功能区域称为操纵子,它可能与毗邻的RNA合成开始的RNA聚合酶结合位点重叠。该操纵子允许(可诱导)对转录作负调节,因为基因阻遏蛋白可能结合操纵子并因而抑制特定基因的转录。在负调节元件(如操纵子)不存在时,可能发生组成型表达。另外,正调节可通过基因激活蛋白结合序列来实现,如果有的话,该结合序列通常邻近RNA聚合酶结合序列的(5′)。基因激活蛋白的例子是分解代谢物激活剂蛋白(CAP),它帮助启动大肠杆菌(E.coli)中的lac操纵子的转录[Raibaud等人(1984)Annu.Rev.Genet.18:173]。因此,表达的调控可能是正调控作用或负调控作用,从而增强或减弱转录。

编码代谢途径中的酶的序列提供了特别有用的启动子序列。例子包括衍生白糖(如半乳糖、乳糖(lac)[Chang等人(1977)Nature 198:1056]和麦芽糖)代谢酶的启动子序列。其它例子包括衍生自生物合成酶(如色氨酸(trp))[Goeddel等人(1980)Nuc.Acids Res.8:4057;Yelverton等人(1981)Nucl.Acids Res.9:731;美国专利4,738,921;EPO出版物No.036 776和121 775]的启动子序列。β-内酰胺酶(bla)启动子系统[Weissmann(1981)″干扰素的克隆和其它错误″《干扰素3》(I.Gresser编辑)],λ嗜菌体PL[Shimatake等人(1981)Nature 292:128]和T5[美国专利4,689,406]启动子系统也提供了有用的启动子序列。

另外,非天然存在的合成的启动子也可象细菌启动子一样起作用。例如,一种细菌或嗜菌体启动子的转录激活序列可以和另一种细菌或嗜菌体启动子的操纵子序列连接在一起,形成合成的杂交启动子[美国专利4,551,433]。例如,tac启动子是杂合的trp-lac启动子,它由trp启动子以及受lac阻遏蛋白调节的lac操纵子序列组成[Amann等人(1983)Gene 25:167;de Boer等人,(1983)Proc.Natl.Acad.Sci.80:21]。另外,细菌启动子可包括非细菌来源但能结合细菌RNA聚合酶并启动转录的天然存在的启动子。天然存在的非细菌来源的启动子还能和相容的RNA聚合酶偶联在一起,从而在原核细胞中高水平地表达某些基因。噬菌体T7 RNA聚合酶/启动子系统是一种偶联的启动子系统[Studier等人(1986)J.Mol.Biol.189:113;Tabor等人(1985)Proc.Natl.Acad.Sci.82:1074]。另外,杂合的启动子还可由嗜菌体启动子以及大肠杆菌操纵子区域组成(EPO出版物No.267 851)。

除了有功能的启动子序列外,有效的核糖体结合位点对于外来基因在原核细胞中的表达也是有用的。在大肠杆菌中,核糖体结合位点称为Shine-Dalgarno(SD)序列,其包括起始密码子(ATG)以及在起始密码子上游3-11个核苷酸处的长度为3-9个核苷酸的序列[Shine等人(1975)Nature 254:34]。认为SD序列是通过SD序列和大肠杆菌16SrRNA的3′端之间碱基配对来促进mRNA与核糖体结合的[Steitz等人(1979)″信使RNA中的遗传信号和核苷酸序列″生物学调节和发育:基因表达″(编者R.F.Goldberger)]。为了表达具有弱的核糖体结合位点的原核基因和真核基因,通常需要优化SD序列与真核基因的ATG之间的距离[Sambrook等人(1989)″克隆基因在大肠杆菌中的表达″《分子克隆实验手册》]。

DNA分子可以在胞内表达。启动子序列可以直接与DNA分子相连,在这种情况下,N端的第一个氨基酸始终是甲硫氨酸,其由ATG起始密码子编码。如果需要,可通过和溴化氰体外培育或通过和细菌甲硫氨酸N-端肽酶体内或体外培育,将N端的甲硫氨酸从蛋白质上切下(EPO出版物No.219 237)。

融合蛋白为直接表达提供了另一种方法。通常,将编码内源细菌蛋白或其它稳定的蛋白之N端部分的DNA序列与异源编码序列的5′端融合。在表达时,该构建物将提供这两个氨基酸序列的融合物。例如,λ噬菌体细胞基因可以和外源基因的5′端相连并在细菌中表达。所得融合蛋白宜保留一个酶(因子Xa)加工位点,以便将噬菌体蛋白与外源基因切开[Nagai等人(1984)Nature 309:810]。融合蛋白也可用lacZ[Jia等人(1987)Gene 60:197],trpE[Allen等人(1987)J.Biotechnol.5:93;Makoff等人(1989),J.Gen.Microbiol.135:11]以及Chey[EPO出版物No.324 647]基因的序列制备。两个氨基酸序列连接处的DNA序列可以编码或不编码一可切割的位点。另一个例子是遍在蛋白融合蛋白。这种融合蛋白由遍在蛋白区域组成,该区域宜保留一个酶(例如遍在蛋白特异性加工蛋白酶)加工位点,以便将外源蛋白和遍在蛋白切开。通过这种方法,可以分离获得天然的外源蛋白[Miller等人(1989)Bio/Technology 7:698]。

另外,还可通过产生嵌合的DNA分子来将外源蛋白分泌出细胞,该嵌合的DNA分子编码的融合蛋白含有一个信号肽序列片段,该序列片段能使细菌中的外源蛋白分泌出来[美国专利4,336,336]。信号序列片段通常编码一个信号肽,该信号肽含有疏水性氨基酸,能指引蛋白分泌出细胞。蛋白质被分泌到生长培养基(革兰阳性菌)中或细胞内膜和外膜之间的周质间隙内(革兰阴性菌)。在编码信号肽片段的基因和外源基因之间宜具有能在体内或体外切割的加工位点。

编码合适信号序列的DNA可以从分泌性细菌蛋白的基因衍生获得,这些基因例如是大肠杆菌外膜蛋白基因(ompA)[Masui等人(1983),《基因表达的实验操作》;Ghrayeb等人(1984)EMBO J.3:2437]以及大肠杆菌碱性磷酸酶信号序列(phoA)[Oka等人(1985)Proc.Natl.Acad.Sci.82:7212]。另一个例子是,可采用各种芽孢杆菌菌株的α淀粉酶基因的信号序列将异源蛋白分泌出枯草芽孢杆菌[Palva等人(1982)Proc.Natl.Acad.Sci.USA 79:5582;EPO出版物No.244 042]。

通常,细菌所识别的转录终止序列是位于翻译终止密码子3′的调控区,它和启动子一起侧接在编码序列的两侧。这些序列指导mRNA的转录,而mRNA能被翻译成该DNA所编码的多肽。转录终止序列通常包括约50个核苷酸的DNA序列,该序列能形成帮助终止转录的茎环结构。例子包括衍生自具有强启动子的基因(如大肠杆菌中的trp基因以及其它生物合成的基因)的转录终止序列。

上述组件,包括启动子、信号序列(如果需要的)、感兴趣的编码序列以及转录终止序列通常一起被放在表达构建物中。表达构建物通常以复制子的形式维持,例如能在宿主(如细菌)中稳定维持的染色体外元件(如质粒)。复制子具有一个复制系统,从而允许其维持在原核宿主中或进行表达或进行克隆和扩增。另外,复制子可以是高拷贝数或低拷贝数的质粒。高拷贝数质粒的拷贝数大致在约5至200之间,通常在约10至150之间。含有高拷贝数质粒的宿主宜含有至少约10个质粒,更佳的含有至少约20个质粒。根据载体以及外源蛋白对宿主的影响,可以选择高拷贝数或低拷贝数的载体。

另外,表达构建物可以和一个整合载体一起整合入细菌基因组中。整合载体通常含有至少一个序列,还序列与细菌染色体同源,从而允许该载体整合。整合看来是载体和细菌染色体中的同源DNA之间重组的结果。例如,用不同芽孢杆菌菌株的DNA构建的整合载体整合到芽孢杆菌染色体中(EPO出版物No.127 328)。整合载体还可包含噬菌体或转座子序列。

通常,染色体外构建物以及整合的表达构建物可含有可选择的标记,以便选择已经转化的菌株。可选择标记可在细菌宿主中表达,其包括赋予细菌对药物(如氨苄青霉素、氯霉素、红霉素、卡那霉素(新霉素)和四环素)抗性的基因[Davies等人(1978)Annu.Rev.Microbiol.32:469]。可选择标记还可包括生物合成性基因,如在组氨酸、色氨酸以及亮氨酸生物合成途径中的那些基因。

另外,上述某些组件可以一起放在转化载体中。转化载体通常包含一个可选择标记,如上所述,该载体以复制子形式维持或发展成一个整合载体。

已经开发出了用于转化到许多细菌中的表达和转化载体(无论是染色体外复制子还是整合载体)。例如,已经开发出了用于下列细菌的表达载体:枯草芽孢杆菌[Palva等人,(1982)Proc.Natl.Acad.Sci.USA 79:5582;EPO出版物No.036 259和063 953;PCT出版物WO 84/04541],大肠杆菌[Shimatake等人,(1981)Nature 292:128;Amann等人,(1985)Gene 40:183;Studier等人,(1986)J.Mol.Biol.189:113;EPO出版物No.036776、136 829和136 907],酪链球菌[Powell等人,(1988)Appl.Environ.Microbiol.54:655];浅青紫链球菌[Powell等人,(1988)Appl.Environ.Microbiol.54:655],浅青紫链霉菌[USpatent 4,745,056].

将外源DNA导入细菌宿主的方法是本领域熟知的,通常包括用氯化钙或其它试剂(如二价阳离子和DMSO)处理对细菌进行转化。DNA还可通过电穿孔方法导入细菌细胞。转化程序通常因待转化的细菌种类而不同。[参见,例如使用杆菌:Masson等人,(1989)FEMS Microbiol.Lett.60:273;Palva等人,(1982)Proc.Natl.Acad.Sci.USA79:5582;EPO出版物No.036 259和063 953;PCT出版物WO 84/04541;使用弯曲杆菌:Miller等人,(1988)Proc.Natl.Acad.Sci.85:856;和Wang等人,(1990)J.Bacteriol.172:949;使用埃希氏大肠杆菌:Cohen等人,(1973)Proc.Natl.Acad.Sci.69:2110;Dower等人,(1988)Nucleic Acids Res.16:6127;Kushner(1978)″用ColE1-衍生质粒转化大肠杆菌的改进方法″Genetic Engineering:Proceedings of the International Symposium onGenetic Engineering(H.W.Boyer和S.Nicosia编辑);Mandel等人,(1970)J.Mol.Biol.53:159;Taketo(1988)Biochim.Biophys.Acta 949:318;使用乳酸杆菌:Chassy等人,(1987)FEMS Microbiol.Lett.44:173;使用假单胞菌:Fiedler等人,(1988)Anal.Biochem 170:38;使用葡萄球菌:Augustin等人,(1990)FEMS Microbiol.Lett.66:203;使用链球菌:Barany等人,(1980)J.Bacteriol.144:698;Harlander(1987)″用电穿孔转化链球菌产乳酸微生物″Streptococcal Genetics(J.Ferretti和R.Curtiss III编辑);Perry等人,(1981)Infect.Immun.32:1295;Powell等人,(1988)Appl.Environ.Microbiol.54:655;Somkuti等人,(1987)Proc.4th Evr.Cong.Biotechnology 1:412]。

v.酵母表达酵母表达系统也是本领域普通技术人员所知的。酵母启动子是能结合酵母RNA聚合酶并启动下游(3′)编码序列(如结构基因)转录成mRNA的DNA序列。启动子具有一个转录起始区,它通常位于编码序列的5′端附近。该转录起始区通常包括RNA聚合酶结合位点(″TATA″盒)以及一个转录起始位点。酵母启动子可能还有第二个功能区域称为上游激活序列(UAS),如果存在的话,它通常远离结构基因。UAS能调节表达(可诱导)。在UAS不存在时,发生组成型表达。表达的调控可能是正调控作用或负调控作用的,从而增强或减弱转录。

酵母是一种发酵生物体,具有活泼的代谢途径,因此编码代谢途径中的酶的序列提供了特别有用的启动子序列。例子包括醇脱氢酶(ADH)(EPO出版物No.284 044)、烯醇酶、葡萄糖激酶、葡萄糖-6-磷酸异构酶、甘油醛-3-磷酸-脱氢酶(GAP或GAPDH)、己糖激酶、磷酸果糖激酶、3-磷酸甘油酸变位酶、以及丙酮酸激酶(PyK)(EPO出版物No.329 203)。编码酸性磷酸酶的酵母PHO5基因也提供了有用的启动子序列[Myanohara等人(1983)Proc.Natl.Acad.Sci.USA 80:1]。

另外,非天然存在的合成的启动子也可象酵母启动子一样起作用。例如,一种酵母启动子的UAS序列可以和另一种酵母启动子的转录激活区连接在一起,形成合成的杂合启动子。这种杂合启动子的例子包括与GAP转录激活区相连的ADH调控序列(美国专利No.4,876,197和4,880,734)。杂合启动子的其它例子包括由ADH2、GAL4、GAL10或PHO5基因的调控序列组成的启动子与糖酵解酶基因如GAP或PyK的转录激活区的组合(EPO出版物No.164 556)。另外,酵母启动子可包括非酵母来源但能结合酵母RNA聚合酶并启动转录的天然存在的启动子。这些启动子的例子包括,尤其是,[Cohen等人,(1980)Proc.Natl.Acad.Sci.USA 77:1078;Henikoff等人,(1981)Nature 283:835;Hollenberg等人,(1981)Curr.Topics Microbiol.Immunol.96:119;Hollenberg等人,(1979)″细菌抗生素抗性基因在酿酒酵母中的表达″Plasmids of Medical,Environmentaland Commercial Importance(K.N.Timmis和A.Puhler编辑);Mercerau-Puigalon等人,(1980)Gene 11:163;Panthier等人,(1980)Curr.Genet.2:109]。

DNA分子可以在酵母菌胞内表达。启动子序列可以直接与DNA分子相连,在这种情况下,重组蛋白N端的第一个氨基酸始终是甲硫氨酸,其由ATG起始密码子编码。如果需要,可通过和溴化氰体外培育将N端的甲硫氨酸从蛋白质上切下。

象在哺乳动物、植物、杆状病毒以及细菌表达系统中一样,融合蛋白为酵母表达系统提供了另一种方法。通常,将编码内源酵母蛋白或其它稳定的蛋白之N端部分的DNA序列与异源编码序列的5′端融合。在表达时,该构建物将提供这两个氨基酸序列的融合物。例如,酵母或人超氧化物歧化酶(SOD)基因可以和外源基因5′端相连并在酵母中表达。两个氨基酸序列连接处的DNA序列可以编码或不编码可切割的位点。例如参见EPO出版物No.196 056。另一个例子是遍在蛋白融合蛋白。这种融合蛋白由遍在蛋白区域组成,该区域宜保留一个酶(例如遍在蛋白特异性加工蛋白酶)加工位点,以便将外源蛋白和遍在蛋白切开。因此,通过这种方法,可以分离获得天然的外源蛋白(例如WO88/024066)。

另外,还可通过产生嵌合的DNA分子来将外源蛋白从细胞分泌到生长培养基中,该嵌合的DNA分子编码的融合蛋白含有一个前导序列片段,该前导序列片段能使酵母中的外源蛋白分泌出来。较佳的,在编码前导片段的基因和外来基因之间宜具有能在体内或体外切割的加工位点。该前导序列片段通常编码了含有疏水性氨基酸的信号肽,其引导蛋白从细胞分泌出来。

编码合适信号序列的DNA可以从分泌性酵母蛋白的基因衍生获得,这些基因例如有酵母转化酶基因(EPO出版物No.012 873;JPO出版物.62:096,086)以及A-因子基因(美国专利4,588,684)。另外,非酵母来源的前导序列(如干扰素前导序列)的存在也能提供分泌出酵母的作用(EPO出版物No.060 057)。

较佳的一类分泌前导序列采用了酵母α-因子基因的片段,其含有″pre″信号序列和″pro″区。可采用的α因子片段的类型包括全长pre-pro α因子前导序列(约83个氨基酸残基)以及截短的α-因子前导序列(通常约25至50个氨基酸残基)(美国专利4,546,083和4,870,008;EPO出版物No.324 274)。采用α-因子前导片段提供分泌作用的其它前导序列包括杂合的α-因子前导序列,其由第一个酵母的pre序列以及第二个酵母α因子的pro区域组成(例如见PCT出版物WO 89/02463)。

通常,酵母识别的转录终止序列是位于翻译终止密码子3′的调控区,其和启动子一起侧接在编码序列的两侧。这些序列指导mRNA的转录,而mRNA能被翻译成该DNA所编码的多肽。转录终止序列和其它酵母识别的终止序列的例子是编码糖酵解酶的那些转录终止序列。

上述组件,包括启动子、前导序列(如果需要的)、感兴趣的编码序列以及转录终止序列,通常被一起放在表达构建物中。表达构建物通常以复制子的形式保持,例如能在宿主(如酵母或细菌)中稳定保持的染色体外元件(如质粒)。复制子可能具有两个复制系统,从而允许其能维持在例如酵母中进行表达,并能维持在原核宿主进行克隆和扩增。这些酵母-细菌穿梭载体的例子包括YEp24[Botstein等人(1979)Gene 8:17-24],pCL/1[Brake等人,(1984)Proc.Natl.Acad.Sci.USA 81:4642-4646]和YRp17[Stinchcomb等人(1982)J.Mol.Biol.158:157]。另外,复制子可以是高拷贝数或低拷贝数的质粒。高拷贝数质粒的拷贝数大致在约5至200之间,通常在约10至150之间。含有高拷贝数质粒的宿主宜含有至少约10个质粒,更佳的含有至少约20个质粒。根据载体以及外源蛋白对宿主的影响,可以选择高拷贝数或低拷贝数的载体。例如参见Brake等人,同上。

另外,表达构建物可以和一个整合载体一起整合入酵母基因组中。整合载体通常含有至少一个序列,该序列与酵母染色体同源,从而允许该载体整合,最好含有两个同源序列侧接该表达构建物。整合看来是载体和酵母染色体中同源DNA之间重组的结果[Orr-Weaver等人(1983)Methods in Enzymol.101:228-245]。通过选择合适的同源序列插入载体中,可将整合载体导入酵母中某一特定的基因座。见Orr-Weaver等人,同上。可以整合入一个或多个表达构建物,这可能会影响重组蛋白产生的水平[Rine等人(1983)Proc.Natl.Acad.Sci.USA 80:6750]。载体中的染色体序列可以载体中的单个片段形式存在(从而导致整个载体的整合),或是与染色体中的相邻片段同源的两个片段,这两个片段在载体中侧接在表达构建物两侧,从而可导致仅表达构建物的稳定性整合。

通常,染色体外表达构建物以及整合的表达构建物均含有可选择的标记,以便选择已经转化的酵母菌株。可选择标记可包括能在酵母宿主中表达的生物合成基因(如ADE2、HIS4、LEU2、TRP1和ALG7以及G418抗性基因),这些基因分别赋予酵母细胞对衣霉素以及G418的抗性。另外,合适的可选择标记还可能为酵母在毒性化合物(如金属)存在下提供生长能力。例如,CUP1的存在使酵母能在铜离子存在下生长[Butt等人,(1987)Microbiol,Rev.51:351]。

另外,上述某些组件可以一起放在转化载体中。转化载体通常包含一个可选择标记,如上所述,该载体以复制子形式维持或发展成一个整合载体。

已经开发出了用于转化入许多酵母中的表达和转化载体(无论是染色体外复制子还是整合载体)。例如,已经开发出用于下列酵母菌的表达载体和将外源DNA导入酵母宿主的方法:白色念珠菌[Kurtz,等人,(1986)Mol.Cell.Biol.6:142],麦芽糖念珠菌[Kunze,等人,(1985)J.Basic Microbiol.25:141],多形汉逊酵母[Gleeson,等人,(1986)J.Gen.Microbiol.132:3459;Roggenkamp等人,(1986)Mol.Gen.Genet.202:302],脆壁克鲁维酵母[Das,等人,(1984)J.Bacteriol.158:1165],乳酸克鲁维酵母[DeLouvencourt等人,(1983)J.Bacteriol.154:737;Van den Berg等人,(1990)Bio/Technology 8:135],季也蒙毕赤酵母[Kunze等人,(1985)J.Basic Microbiol.25:141],巴斯德毕酵母[Cregg,等人,(1985)Mol.Cell.Biol.5:3376;美国专利No.4,837,148和4,929,555],酿酒酵母[Hinnen等人,(1978)Proc.Natl.Acad.Sci.USA 75:1929;Ito等人,(1983)J.Bacteriol.153:163],栗酒裂植酵母[Beach和Nurse(1981)Nature 300:706],以及Yarrowia lipolytica[Davidow,等人,(1985)Curr.Genet.10:380471 Gaillardin,等人,(1985)Curr.Genet.10:49]。

将外源DNA导入酵母宿主的方法是本领域熟知的,通常包括用碱阳离子处理转化原生质球或完整酵母细胞。转化程序通常因待转化的酵母种类而不同。例如参见,[Kurtz等人,(1986)Mol.Cell.Biol.6:142;Kunze等人,(1985)J.Basic Microbiol.25:141;念珠菌];[Gleeson等人,(1986)J.Gen.Microbiol.132:3459;Roggenkamp等人,(1986)Mol.Gen.Genet.202:302;汉逊酵母];[Das等人,(1984)J.Bacteriol.158:1165;DeLouvencourt等人,(1983)J.Bacteriol.154:1165;Van den Berg等人,(1990)Bio/Technology 8:135;克鲁维酵母];[Cregg等人,(1985)Mol.Cell.Biol.5:3376;Kunze等人,(1985)J.Basic Microbiol.25:141;美国专利No.4,837,148和4,929,555;毕赤酵母];[Hinnen等人,(1978)Proc.Natl.Acad.Sci.USA 75;1929;Ito等人,(1983)J.Bacteriol.153:163酿酒酵母];[Beach和Nurse(1981)Nature 300:706;裂殖酵母];[Davidow等人,(1985)Curr.Genet.10:39;Gaillardin等人,(1985)Curr.Genet.10:49;Yarrowia]。

定义当组合物中总X+Y重量的至少85%是X时,则称含有X的组合物“基本上没有Y”。较佳的,X占组合物中X+Y总重量的至少约90%,更佳至少约95%或者甚至99%(重量)。

术语“异源”指在自然界中发现不在一起的两种生物学组分。此组分可以是宿主细胞、基因、或调控区如启动子。尽管异源组分在自然界中发现不在一起,但是它们能一起起作用,例如当与某基因异源的一种启动子与该基因操作性相连时。另一个例子是奈瑟球菌序列与小鼠宿主细胞异源。

“复制起点”是启动和调节多核苷酸(例如表达载体)复制的一种多核苷酸序列。复制起点可作为细胞内多核苷酸复制的自主性单位,能在其自身的控制下进行复制。复制起点是载体在特定宿主细胞中复制所需要的。有了某一复制起点,表达载体就能在细胞中合适蛋白的存在下高拷贝数的复制。复制起点的例子是在酵母中有效的自主复制序列;以及在COS-7细胞中有效的病毒性T-抗原。

“突变体”序列定义为与天然或公开的序列不同但具有同源性的DNA、RNA或氨基酸序列。根据具体的序列,天然或公开的序列与突变体序列之间的同源性程度宜大于50%(例如60%、70%、80%、90%、95%、99%或更高),其中同源性用上述方法计算出。如本文所述,本文提供的核酸序列的核酸分子或区域的“等位基因变体”是在另一或第二个分离物的基因组中基本上相同的基因座上的核酸分子或区域,由于诸如突变或重组引起的自然变异,它们具有相似但不相同的核酸序列。编码区等位基因变体通常编码的蛋白具有与其比较基因所编码蛋白相似的活性。等位基因变体还可包含基因5′或3′非翻译区中的变化,例如在调节控制区中的变化(例如见美国专利5,753,235)。

抗体本文所用的术语“抗体”指由至少一个抗体结合位点组成的一个或一组多肽。“抗体结合位点”是一个三维结合空间,其内表面形状和电荷分布与抗原表位的特征互补,从而使抗体与抗原结合。“抗体”例如包括,脊椎动物抗体、杂合抗体、嵌合抗体、人源化抗体、经修饰的抗体、单价抗体、Fab蛋白以及单结构域抗体。

针对本发明蛋白的抗体可用于亲和层析、免疫试验以及区别/鉴定奈瑟球菌menB蛋白。所引发的针对本发明蛋白的抗体,可结合于存在的且与脑膜炎奈瑟球菌menB菌株特异性相关的抗原性多肽或蛋白质或蛋白片段。在某些情况下,这些抗原与特定的菌株相关,例如那些menB菌株特有的抗原。本发明的抗体可以固定于基质并用于免疫试验,或者固定在亲和层析柱上以检测和/或分离多肽、蛋白或蛋白片段,或者含所述多肽、蛋白或蛋白片段的细胞。或者,所述多肽、蛋白或蛋白片段可以被固定化,以检测能与其特异性结合的抗体。

针对本发明蛋白的多克隆和单克隆抗体可用常规方法制得。通常,首先用蛋白来免疫合适的动物,较佳的是小鼠、大鼠、家兔或山羊。由于可获得的血清体积多,能获得标记的抗家兔和抗山羊抗体,因此对于制备多克隆抗血清来说,家兔和山羊是较佳的。免疫通常这样进行:将蛋白以盐水(较佳的以佐剂如Freund氏完全佐剂)混合或乳化,然后肠胃外(通常是皮下或肌内)注射该混合物或乳剂。每次注射50-200微克的剂量就足够了。2-6周后用盐水(较佳的是用Freund氏不完全佐剂)配的蛋白质注射一次或多次以强化免疫。另外可以用本领域已知的方法进行体外免疫来产生抗体,从本发明的目的来看,认为其与体内免疫等效。将免疫后的动物血液抽取到玻璃或塑料容器中,25℃培育该血液1小时,然后4℃培育2-18小时,获得多克隆抗血清。离心(例如1000g 10分钟)回收血清。家兔每次取血可获得约20-50毫升。

用Kohler和Milstein的标准方法[Nature(1975)256:495-96]或其改进方法制得单克隆抗体。通常,如上所述免疫小鼠或大鼠。然而,不是对动物取血然后抽提血清,而是取出脾脏(以及任选地取出几个大的淋巴结),将其分离成单细胞。如果需要,可将细胞悬液(在除去非特异性粘附的细胞后)加入包被了蛋白质抗原的板或孔中,对脾细胞进行筛选。表达抗原特异性的膜结合免疫球蛋白的B细胞结合到板上,不象悬液其它物质那样被洗去。然后使所得B细胞或所有解离的脾细胞与骨髓瘤细胞融合形成杂交瘤,培养在选择性培养基(如次黄嘌呤、氨基蝶呤胸苷培养基,“HAT”)中。通过有限稀释接种所得杂交瘤,并测定特异性结合免疫抗原(且不结合无关抗原)的抗体的产生。然后,体外(例如在组织培养瓶或中空纤维反应器中)或体内(如小鼠腹水中)培养所选的分泌单克隆抗体的杂交瘤。

如果需要,抗体(无论是多克隆还是单克隆抗体)可用常规技术来标记。合适的标记包括荧光团、发色团、放射活性原子(具体是32P和125I)、密电子试剂、酶、以及具有特异性结合配偶的配体。酶通常靠其活性来检测。例如,辣根过氧化物酶通常是检测其将3,3′,5,5′-四甲基联苯胺(TMB)转变成蓝色的能力,可用分光光度计定量测定。“特异性结合配偶”指能以高特异性结合配体分子的蛋白质,例如抗原以及对其有特异性的单克隆抗体。其它特异性结合配偶包括生物素和亲和素或链霉亲和素,IgG和蛋白A,以及本领域已知的许多受体-配体对。应理解,上述内容并非要将各种标记分成不同的类,因为同一标记可在几种不同的模型中起作用。例如,125I可作为放射活性标记,或作为密电子试剂。HRP可作为酶或单抗的抗原。另外,一种物质可以和各种标记组合以获得所需的效果。例如,在实施本发明中,单抗和亲和素也需要标记,因此,可以用生物素标记单抗,并用标记了125I的亲和素检测其存在,或用标记HRP的抗生物素单抗检测其存在。其它替换和可能性对于本领域普通技术人员来说是显而易见的,所以应认作等价物属于本发明的范围。

本发明的抗原、免疫原、多肽、蛋白或蛋白片段可引发形成特异结合配对(partner)抗体。本发明的这些抗原、免疫原、多肽、蛋白或蛋白片段包括本发明的免疫原性组合物。这些免疫原性组合物还可包括或含有佐剂、载体、或其他促进或增强或稳定本发明抗原、多肽、蛋白或蛋白片段的组份。这些佐剂和载体对本领域一般技术人员而言是很显而易见的。

药物组合物药物组合物可包含本发明的多肽、抗体或核酸。该药物组合物将包含治疗有效量的本发明的多肽、抗体或多核苷酸。

本文所用的术语“治疗有效量”指治疗剂治疗、缓解或预防目标疾病或状况的量,或是表现出可检测的治疗或预防效果的量。该效果例如可通过化学标记或抗原水平来检测。治疗效果也包括生理性症状的减少,例如对发热病人给药时导致体温降低。对于某一对象的精确有效量取决于该对象的体型和健康状况、病症的性质和程度、以及选择给予的治疗剂和/或治疗剂的组合。因此,预先指定准确的有效量是没用的。然而,对于某给定的状况而言,可以用常规实验来确定该有效量,临床医师是能够判断出来的。

为了本发明的目的,有效的剂量为给予个体约0.01毫克/千克至50毫克/千克或0.05毫克/千克至10毫克/千克的DNA构建物。

药物组合物还可含有药学上可接受的载体。术语“药学上可接受的载体”指用于治疗剂(例如抗体、多肽、基因或其它治疗剂)给药的载体。该术语指这样一些药剂载体:它们本身不诱导产生对接受该组合物的个体有害的抗体,且给药后没有过分的毒性。合适的载体可能是大的、代谢缓慢的大分子,如蛋白质、多糖、聚乳酸(polylactic acid)、聚乙醇酸、氨基酸聚合物、氨基酸共聚物以及无活性的病毒颗粒。这些载体是本领域普通技术人员所熟知的。

本文可用的药学上可接受的盐例如有:无机酸盐,如盐酸盐、氢溴酸盐、磷酸盐、硫酸盐等;以及有机酸盐,如乙酸盐、丙酸盐、丙二酸盐、苯甲酸盐等。在Remington′sPharmaceutical Sciences(Mack Pub.Co.,N.J.1991)中可找到关于药学上可接受的赋形剂的充分讨论。

治疗性组合物中药学上可接受的载体可含有液体,如水、盐水、甘油和乙醇。另外,这些载体中还可能存在辅助性的物质,如润湿剂或乳化剂、pH缓冲物质等。通常,可将治疗性组合物制成可注射剂,例如液体溶液或悬液;还可制成在注射前适合配入溶液或悬液中、液体载体的固体形式。脂质体也包括在药学上可接受的载体的定义中。

输药方法一旦配成本发明的组合物,可将其直接给予对象。待治疗的对象可以是动物;尤其可以治疗人对象。

直接输送该组合物通常可通过皮下、腹膜内、静脉内或肌内注射或输送至组织间隙来实现。组合物也可输送至病灶区。其它给药方式包括口服和肺给药、栓剂和透皮或经皮肤施用、用针、基因枪或手持喷雾器(hypospray)。治疗剂量方案可以是单剂方案或多剂方案。

疫苗本发明的疫苗可以是预防性的(即预防感染)或治疗性的(即在感染后治疗疾病)。

这些疫苗包含免疫性抗原或免疫原、免疫原性多肽、蛋白或蛋白片段、或核酸(如核糖核酸或脱氧核糖核酸),通常与“药学上可接受的载体”组合,这些载体包括本身不诱导产生对接受该组合物的个体有害的抗体的任何载体。合适的载体通常是大的、代谢缓慢的大分子,如蛋白质、多糖、聚乳酸、聚乙醇酸、氨基酸聚合物、氨基酸共聚物、脂质凝集物(如油滴或脂质体)以及无活性的病毒颗粒。这些载体是本领域普通技术人员所熟知的。另外,这些载体可起免疫刺激剂(“佐剂”)作用。另外,抗原或免疫原可以和细菌类毒素(如白喉、破伤风、霍乱、幽门螺杆菌等病原体的类毒素)偶联。

增强组合物效果的较佳的佐剂包括但不局限于:(1)铝盐(alum),如氢氧化铝、磷酸铝、硫酸铝等;(2)水包油型乳剂配方(有或没有其它特异性的免疫刺激剂,如胞壁酰肽(见下文)或细菌细胞壁成分),例如,(a)MF59(PCT出版物No.WO 90/14837),其含有5%鲨烯、0.5%吐温80和0.5%Span 85(任选地含有不同量的MTP-PE(见下文),虽然并不需要),配制入用微量流化器(如110Y型微量流化器(Microfluidics,Newton,MA))制成的亚微米级颗粒中;(b)SAF,其含有10%鲨烯、0.4%吐温80、5%普卢兰尼克(pluronic)嵌段聚合物L121以及thr-MDP(见下文),微量流化成亚微米级乳剂或涡流振荡产生粒径较大的乳剂,和(c)RibiTM佐剂系统(RAS)(Ribi Immunochem,Hamilton,MT),其含有2%鲨烯、0.2%吐温80以及取自单磷酰脂A(MPL)、二霉菌酸海藻糖酯(TDM)、和细胞壁骨架(CWS)的一种或多种细菌细胞壁组分,较佳的是MPL+CWS(DetoxTM);(3)皂素佐剂,例如可采用StimulonTM(Cambridge Bioscience,Worcester,MA)或从其产生的颗粒,如ISCOM(免疫刺激性复合物);(4)Freund完全佐剂(CFA)和Freund不完全佐剂(IFA);(5)细胞因子,如白介素(如IL-1、IL-2、IL-4、IL-5、IL-6、IL-7、IL-12等)、干扰素(如γ干扰素)、巨噬细胞集落刺激因子(M-CFS)、肿瘤坏死因子(TNF)等;(6)细菌ADP-核糖基化毒素(如霍乱毒素CT,百日咳毒素PT或大肠杆菌热不稳定毒素LT)的脱毒变异体,尤其是LT-K63、LT-R72,CT-S109、PT-K9/G129;参见例如WO93/13302和WO92/19265;以及(7)作为免疫刺激剂来增强组合物效果的其它物质。Alum和MF59是较佳的。

如上所述,胞壁酰肽包括但不局限于,N-乙酰-胞壁酰-L-苏氨酰-D-异谷氨酰胺(thr-MDP)、N-乙酰-去胞壁酰-L-丙氨酰-D-异谷氨酰胺(nor-MDP)、N-乙酰胞壁酰-L-丙氨酰-D-异谷氨酰氨酰基-L-丙氨酸-2-(1′-2′-二棕榈酰-sn-甘油-3-羟基磷酰氧)-乙胺(MTP-PE)等。

包括免疫原性组合物在内的疫苗组合物(如可包括抗原,药学上可接受的载体以及佐剂),通常含有稀释剂,如水,盐水,甘油,乙醇等。另外,辅助性物质,如润湿剂或乳化剂、pH缓冲物质等可存在于这类运载体中。此外,包括免疫原性组合物在内的疫苗组合物可含有在药学上可接受载体中的抗原、多肽、蛋白、蛋白片段或核酸。

更具体地,包括免疫原性组合物在内的疫苗,包含免疫学有效量的免疫原性多肽,以及上述其它所需的组分。“免疫学有效量”指以单剂或连续剂一部分给予个体的量对治疗或预防是有效的。该用量根据所治疗个体的健康状况和生理状况、所治疗个体的类别(如非人灵长类等)、个体免疫系统合成抗体的能力、所需的保护程度、疫苗的配方、治疗医师对医疗状况的评估、及其它的相关因素而定。预计该用量将在相对较宽的范围内,可通过常规实验来确定。

通常,可将疫苗组合物或免疫原性组合物制成可注射剂,例如液体溶液或悬液;还可制成在注射前适合配入溶液或悬液、液体赋形剂的固体形式。该制剂还可乳化或包封在脂质体中,在上述药学上可接受的载体下增强佐剂效果。

常规方法是从肠胃外(皮下或肌内)途径通过注射给予该免疫原性组合物。适合其它给药方式的其它配方包括口服和肺制剂、栓剂和经皮与透皮应用。治疗剂量可以是单剂方案或多剂方案。疫苗可以结合其它免疫调节剂一起给予。

作为以蛋白质为基础的疫苗的一种替代方案是,可以采用DNA疫苗接种[例如,Robinson和Torres(1997)Seminars in Immunology 9:271-283;Donnelly等人(1997)AnnuRev.Immunol 15:617-648]。

基因输送载体用于输送构建物的基因治疗载体可以口服或全身性给予,其中所述构建物包括本发明治疗剂的编码序列,将其输送至哺乳动物以便在哺乳动物体内表达。这些构建物可利用体内或活体外方式中的病毒或非病毒载体方法。这些编码序列的表达可用内源哺乳动物启动子或异源启动子诱导。编码序列的体内表达可以是组成型的或受调控的。

本发明包括能表达所涉及的核酸序列的基因输送运载体。基因输送运载体宜为病毒载体,更佳的是逆转录病毒、腺病毒、腺伴随病毒(AAV)、疱疹病毒或甲病毒载体。病毒载体还可以是星状病毒、冠状病毒、正粘病毒、乳多空病毒、副粘病毒、细小病毒、小核糖核酸病毒、痘病毒或披膜病毒的病毒载体。通常参见Jolly(1994)Cancer GeneTherapy 1:51-64;Kimura(1994)Human Gene Therapy 5:845-852;Connelly(1995)HumanGene Therapy 6:185-193;以及Kaplitt(1994)Nature Genetics 6:148-153。

逆转录病毒载体是本领域中熟知的,包括B、C和D型逆转录病毒、异嗜性逆转录病毒(例如NZB-X1、NZB-X2和NZB9-1(见O′Neill(1985)J.Virol.53:160)广嗜性逆转录病毒如MCF和MCF-MLV(见Kelly(1983)J.Virol 45:291)、泡沫病毒和慢病毒。见《RNA肿瘤病毒》第2版,Cold Spring Harbor Laboratory,1985。

逆转录病毒基因治疗载体的诸部分可从不同逆转录病毒衍生获得。例如,逆转录载体LTR可以从小鼠肉瘤病毒衍生获得,tRNA结合位点可以从Rous肉瘤病毒衍生获得,包装信号从小鼠白血病病毒获得,第二链的合成起点从禽类白血病病毒获得。

可将这些重组逆转录病毒载体导入合适的包装细胞系,用来产生转导感受态逆转录病毒载体颗粒(见美国专利5,591,624)。通过将嵌合性整合酶掺入逆转录病毒颗粒,构建逆转录病毒载体,以便将其定点整合到宿主细胞DNA中(见WO96/37626)。较佳的重组病毒载体是复制缺陷型重组病毒。

适合与上述逆转录病毒载体一起使用的包装细胞系是本领域熟知的,很容易制得(见WO95/30763和WO92/05266),并能用来产生能生产重组载体颗粒的生产型细胞系(也称为载体细胞系或“VCL”)。包装细胞系宜从人亲代细胞(如HT1080细胞)或貂亲代细胞系制取,以便消除人血清的灭活作用。

用来构建逆转录病毒基因治疗载体的较佳的逆转录病毒,包括禽类白血病病毒、牛白血病病毒、鼠白血病病毒、水貂细胞灶诱导病毒、鼠肉瘤病毒、网状内皮组织增殖病毒和Rous肉瘤病毒。特别佳的鼠白血病病毒包括4070A和1504A(Hartley和Rowe(1976)J Virol 19:19-25),Abelson(ATCC No.VR-999),Friend(ATCC No.VR-245),Graffi,Gross(ATCC Nol VR-590),Kirsten,Harvey肉瘤病毒和Rauscher(ATCC No.VR-998)以及莫洛尼鼠白血病病毒(ATCC No.VR-190)。这些逆转录病毒可以从保藏机构或保藏中心如Rockville,Maryland的美国典型培养物保藏中心(ATCC)获得,或用常用的技术从已知来源分离获得。

可用于本发明的典型的已知逆转录病毒基因治疗载体包括在以下专利申请中描述的那些载体:GB2200651,EP0415731,EP0345242,EP0334301,WO89/02468;WO89/05349,WO89/09271,WO90/02806,WO90/07936,WO94/03622,WO93/25698,WO93/25234,WO93/11230,WO93/10218,WO9I/02805,WO9I/02825,WO95/07994,US 5,219,740,US 4,405,712,US 4,861,719,US 4,980,289,US 4,777,127,US 5,591,624.另见Vile(1993)Cancer Res 53:3860-3864;Vile(1993)Cancer Res 53:962-967;Ram(1993)Cancer Res 53(1993)83-88;Takamiya(1992)J Neurosci Res 33:493-503;Baba(1993)J Neurosurg 79:729-735;Mann(1983)Cell 33:153;Cane(1984)Proc Natl Acad Sci81:6349;以及Miller(1990)Human Gene Therapy 1。

人腺病毒基因治疗载体也是本领域中已知的,并可用于本发明。例如参见Berkner(1988)Biotechniques 6:616和Rosenfeld(1991)Science 252:431,以及WO93/07283,WO93/06223和WO93/07282。用于本发明的典型的已知的腺病毒基因治疗载体包括在上述文献以及下述专利中描述的那些例子:WO94/12649,WO93/03769,WO93/19191,WO94/28938,WO95/11984,WO95/00655,WO95/27071,WO95/29993,WO95/34671,WO96/05320,WO94/08026,WO94/11506,WO93/06223,WO94/24299,WO95/14102,WO95/24297,WO95/02697,WO94/28152,WO94/24299,WO95/09241,WO95/25807,WO95/05835,WO94/18922和WO95/09654。另外,可以采用Curiel(1992)Hum.Gene Ther.3:147-154中描述的给予和已杀死腺病毒相连的DNA的方法。本发明的基因输递载体还包括腺病毒伴随病毒(AAV)载体。用于本发明的这种载体的主要且较佳的例子是Srivastava,WO93/09239中公开的AAV-2为基础的载体。最佳的AAV载体包含两个AAV反向末端重复序列,其中通过替换核苷酸对天然D-序列进行修饰,使至少5-18个天然的核苷酸(较佳的至少10-18个天然核苷酸,最佳的10个天然核苷酸)被保留下来,而D-序列其余的核苷酸缺失或用非天然核苷酸取代。AAV反向末端重复序列的天然D-序列是每个AAV反向末端重复序列中不参与HP形成的20个串联核苷酸的序列(即每一端有一个序列)。非天然的替换核苷酸可以是天然D-序列该位置中所见核苷酸以外的任何核苷酸。其它可采用的典型AAV载体是pWP-19、pWN-1,两者均公开在Nahreini(1993)Gene 124:257-262中。这样的AAV的另一个例子是psub201(见Samulski(1987)J.Virol.61:3096)。另一个典型的AAV载体是Double-D ITR载体。Double-D ITR载体的构建方案公开在美国专利5,478,745中。还有其它的载体是公开在Carter的美国专利4,797,368和Muzyczka的美国专利5,139,941、Chartejee的美国专利5,474,935和Kotin的WO94/288157中的载体。可用于本发明的另一个AAV载体例子是SSV9AFABTKneo,它含有AFP增强子和白蛋白启动子,并且主要指导肝内表达。其结构和构建方案公开在Su(1996)Human Gene Therapy 7:463-470中。其它的AAV基因治疗载体在美国专利5,354,678,5,173,414,5,139,941,5,252,479中有所描述。

包含本发明序列的基因治疗载体还包括疱疹载体。主要且较佳的例子是含有编码胸苷激酶多肽的序列的单纯疱疹病毒载体,如公开在US5,288,641和EP0176170(Roizman)中的那些。其它典型的单纯疱疹病毒载体包括WO95/04139中公开的HFEM/ICP6-LacZ(Wistar Institute)、Geller(1988)Science 241:1667-1669以及WO90/09441和WO92/07945中公开的pHSVlac、Fink(1992)Human Gene Therapy 3:11-19中描述的HSV Us3∷pgC-lacZ、EP 0453242(Breakefield)中描述的HSV 7134、2RH 105和GAL4以及保藏于ATCC、保藏号为ATCC VR-977和ATCC VR-260的那些病毒。

还考虑到甲病毒基因基因治疗载体也可用于本发明。较佳的甲病毒载体是新培斯病毒载体。披膜病毒、Semliki Forest病毒(ATCC VR-67;ATCC VR-1247)、Middleberg病毒(ATCC VR-370)、Ross River病毒(ATCC VR-373;ATCC VR-1246)、委内瑞拉马脑炎病毒(ATCC VR923;ATCC VR-1250;ATCC VR-1249;ATCC VR-532)、以及在美国专利5,091,309,5,217,879以及WO92/10578中描述的那些。更具体地说,可以采用1995年3月15日提交的美国申请08/405,627、WO94/21792、WO92/10578、WO95/07994、US 5,091,309和US 5,217,879中描述的那些甲病毒载体。这些甲病毒可以从保藏机构或保藏中心如Rockville,Maryland的美国典型培养物保藏中心(ATCC)获得,或用常用的技术从已知来源分离获得。较佳的是,采用细胞毒性降低的甲病毒载体(见USSN08/679640)。

DNA载体系统,如真核分层的(layered)表达系统也可用于表达本发明的核酸。关于真核分层的表达系统详见WO95/07994。较佳的,本发明的真核分层表达系统宜从甲病毒载体衍生获得,更佳的从新培斯病毒载体衍生获得。

适用于本发明的其它病毒载体包括:从脊髓灰质炎病毒衍生的载体,例如ATCCVR-58以及在Evans,Nature 339(1989)385和Sabin(1973)J.Biol.Standardization 1:115中描述的那些;鼻病毒,例如ATCC VR-1110以及在Arnold(1990)J Cell Biochem L401中描述的那些;痘病毒,如金黄色痘病毒或牛痘病毒,例如ATCC VR-111和ATCCVR-2010,以及在Fisher-Hoch(1989)Proc Natl Acad Sci 86:317;Flexner(1989)Ann NYAcad Sci 569:86,Flexner(1990)Vaccine 8:17;US 4,603,112,US 4,769,330以及WO89/01973中描述的那些;SV40病毒,例如ATCC VR-305以及在Mulligan(1979)Nature 277:108和Madzak(1992)J Gen Virol 73:1533中描述的那些;流感病毒,例如ATCC VR-797以及用例如US 5,166,057和Enami(1990)Proc Natl Acad Sci87:3802-3805;Enami和Palese(1991)J Virol 65:2711-2713;Luytjes(1989)Cell 59:110中所述的反基因技术制得的重组流感病毒(另见McMichael(1983)NEJ Med 309:13,Yap(1978)Nature 273:238以及Nature(1979)277:108);EP-0386882和Buchschacher(1992)J.Virol.66:2731中描述的人免疫缺陷病毒;麻疹病毒,例如ATCC VR-67和VR-1247,以及EP-0440219中描述的那些;奥拉病毒,例如ATCC VR-368;Bebaru病毒,例如ATCC VR-600和ATCC VR-1240;Cabassou病毒,例如ATCC VR-922;屈曲病毒,例如ATCC VR-64和ATCC VR-1241;Fort Morgan病毒,例如ATCC VR-924;Getah病毒,例如ATCC VR-369和ATCC VR-1243;Kyzylagach病毒,例如ATCC VR-927;Mayaro病毒,例如ATCC VR-66;Mucambo病毒,例如ATCC VR-580和ATCC VR-1244;Ndumu病毒,例如ATCC VR-371;Pixuna病毒,例如ATCC VR-372和ATCC VR-1245;Tonate病毒,例如ATCC VR-925;Triniti病毒,例如ATCC VR-469;Una病毒,例如ATCC VR-374;Whataroa病毒,例如ATCC VR-926;Y-62-33病毒,例如ATCC VR-375;O′Nyong病毒,东部脑炎病毒,例如ATCC VR-65和ATCC VR-1242;西部脑炎病毒,例如ATCC VR-70,ATCC VR-1251,ATCC VR-622和ATCC VR-1252;和冠状病毒,例如ATCC VR-740和在Hamre(1966)Proc Soc Exp Biol Med 121:190中描述的那些。

将本发明的组合物输送至细胞内并不局限于上述病毒载体。还可采用其它输送方法和介质,例如核酸表达载体、与已被杀死的腺病毒相连或不相连的单独的聚阳离子凝缩的DNA(例如参见1994年12月30日美国申请No.08/366,787和Curiel(1992)HumGene Ther 3:147-154)、配体连接的DNA(例如参见Wu(1989)J.Biol.Chem.264:16985-16987)、真核细胞输送载体细胞(例如参见1994年5月9日提交的美国申请No.08/240,030以及美国申请No.08/404,796)、光聚合水凝胶材料的沉淀、手提式基因转移颗粒枪(如美国专利5,149,655所述)、电离辐射(如US5,206,152和WO92/11033所述)、核电荷中和或与细胞膜融合。其它方法在Philip(1994)Mol Cell Biol 14:2411-2418以及Woffendin(1994)Proc.Natl.Acad.Sci.91:1581-1585中有所描述。

可以采用颗粒介导的基因转移,例如参见美国申请No.60/023,867。简言之,可将序列插入含有控制高水平表达的常规序列的常规载体中,然后和合成性基因转移分子一起培育,这些基因转移分子例如是聚合性DNA-结合阳离子(如聚赖氨酸、鱼精蛋白和白蛋白),其与细胞寻靶配体(如脱唾液酸血清类粘蛋白(如Wu和Wu(1987)J.Biol.Chem.262:4429-4432所述)、胰岛素(如Hucked(1990)Biochem Pharmacol 40:253-263所述)、半乳糖(如Plank(1992)Bioconjugate Chem 3:533-539所述)、乳糖或运铁蛋白)相连。

裸DNA也可用于转化宿主细胞。典型的裸DNA导入方法在WO 90/11092和US5,580,859中有所描述。用可生物降解的乳胶珠可以改善摄取效果。在对珠粒的胞吞作用开始后,DNA包被的乳胶珠粒被有效地运输到细胞中。通过处理珠粒以提高其疏水性可进一步改进该方法,从而促进核内体的破坏和将DNA释放到细胞质中。

可作为基因输送运载体的脂质体在US 5,422,120,WO95/13796,WO94/23697,WO91/14445和EP-524,968中有所描述。如USSN 60/023,867中所描述的,在非病毒输送时,可将编码多肽的核酸序列插入含有控制高水平表达的常规序列的常规载体中,然后和合成性基因转移分子一起培育,这些基因转移分子例如是聚合性DNA-结合阳离子(如聚赖氨酸、鱼精蛋白和白蛋白),其与细胞寻靶配体(如脱唾液酸血清类粘蛋白、胰岛素、半乳糖、乳糖或运铁蛋白)相连。其它输送系统包括采用脂质体来包裹DNA,该DNA所含基因在各种组织特异性或活性普遍存在的启动子控制下。适用的其它非病毒输送系统包括机械输送系统,如Woffendin等人(1994)Proc.Natl.Acad.Sci.USA91(24):11581-11585中描述的方法。另外,这类编码序列和表达产物可以通过光聚合的水凝胶材料的沉淀来输送。可用来输送编码序列的其它基因输送常规方法例如包括,用手提式基因转移颗粒枪(如美国专利5,149,655所述);用电离辐射来激活转移的基因(如US 5,206,152和WO92/11033所述)。

典型的脂质体和聚阳离子基因输送载体在下列文献中有所描述:US 5,422,120和4,762,915;WO 95/13796;WO94/23697;WO91/14445;EP-0524968;Stryer,Biochemistry,236-240页(1975)W.H.Freeman,San Francisco;Szoka(1980)Biochem Biophys Acta 600:1;Bayer(1979)Biochem Biophys Acta 550:464;Rivnay(1987)Meth Enzymol 149:119;Wang(1987)Proc Natl Acad Sci 84:7851;Plant(1989)Anal Biochem 176:420。

多核苷酸组合物可包含治疗有效量的基因治疗运载体,其定义如上所述。出于本发明的目的,有效的剂量是给予个体约0.01毫克/千克至50毫克/千克或0.05毫克/千克至10毫克/千克的DNA构建物。

输送方法一旦配制后,本发明的多核苷酸组合物可以以下三种方式给予:(1)直接给予对象;(2)活体外输送给对象衍生的细胞;或(3)体外表达重组蛋白。待处理的对象可以是哺乳动物或鸟类。另外,也可对人进行治疗。

直接输送该组合物通常可通过皮下、腹膜内、透皮、经皮、静脉内或肌内注射或输送至组织间隙来实现。该组合物也可输送至肿瘤或病损部位。其它给药方式包括口服和肺给药、栓剂和透皮或经皮肤应用、用针、基因枪或手持喷雾器(hypospray)。治疗剂量方案可以是单剂方案或多剂方案。见WO98/20734。

活体外输送以及将转化的细胞重新植入对象体内的方法是本领域所熟知的,在例如WO93/14778中有所描述。用于活体外应用的细胞例子包括例如干细胞、尤其是造血细胞、淋巴细胞、巨噬细胞、树突细胞或肿瘤细胞。

通常,对于活体外和体外应用,核酸的输送可通过以下步骤来实现,例如葡聚糖介导的转染、磷酸钙沉淀、Polybrene介导的转染、原生质体融合、电穿孔、将多核苷酸包裹在脂质体中以及将DNA直接显微注射到胞核中,所有这些均是本领域所熟知的多核苷酸和多肽药物组合物除了上述的药学上可接受的载体和盐外,多核苷酸和/或多肽组合物中还可采用下列附加试剂。

A.多肽一个例子是多肽,其包括但不局限于:脱唾液酸血清类粘蛋白(ASOR);运铁蛋白;脱唾液酸糖蛋白;抗体;抗体片段;铁蛋白;白介素;干扰素;粒细胞-巨噬细胞集落刺激因子(GM-CSF);粒细胞集落刺激因子(G-CSF)、巨噬细胞集落刺激因子(M-CSF)、干细胞因子和促红细胞生成素。还可使用病毒抗原,如包膜蛋白。另外,可用来自其它侵袭性生物的蛋白,例如疟原虫恶性疟疾的环孢子蛋白的17个氨基酸的肽(称为RII)。

B.激素,维生素等在药物组合物中可包括其它种类,例如是:激素、类固醇、雄激素、雌激素、甲状腺激素或维生素、叶酸。

C.聚亚烷基、多糖等另外,聚(亚烷基)二醇可以和所需的多核苷酸或多肽一起包括在药物组合物中。在一个较佳的实施方案中,聚(亚烷基)二醇是聚乙二醇。另外,可以加入单糖、二糖或多糖。在此方面的一个较佳实施方案中,多糖是葡聚糖或DEAE-葡聚糖。另外,脱乙酰壳多糖和聚交酯-聚乙醇酸内酯共聚物可包括在药物组合物中。

D.脂质和脂质体所需的多核苷酸或多肽还可在输送给对象或对象衍生的细胞之前包裹在脂质中或包裹在脂质体中。

脂质包裹通常用能稳定结合或捕获并保留核酸或多肽的脂质体来实现。凝聚的多核苷酸与脂质制剂之比可以不同,但是通常在约1∶1(毫克DNA∶微摩尔脂质)之间,或脂质更多。关于脂质体作为输送核酸的载体的综述参见Hug和Sleight(1991)Biochim.Biophys.Acta.1097:1-17;Straubinger(1983)Meth.Enzymol.101:512-527。

用于本发明的脂质体制剂包括阳离子(带正电荷)、阴离子(带负电荷)和中性制剂。阳离子脂质体已经显示出能以有功能的形式介导质粒DNA的胞内输送(Felgner(1987)Proc.Natl.Acad.Sci.USA 84:7413-7416);mRNA(Malone(1989)Proc.Natl.Acad.Sci.USA 86:6077-6081);和纯化的转录因子的胞内输送(Debs(1990)J.Biol.Chem.265:10189-10192)。

阳离子脂质体很容易购得。例如,N(1-2,3-二油烯基氧)丙基)-N,N,N-三乙铵(DOTMA)脂质体可以以Lipofectin的商标从GIBCO BRL,Grand Island,NY购得。(另见Felgner,同上)。其它商品脂质体包括transfectace(DDAB/DOPE)和DOTAP/DOPE(Boerhinger)。其它阳离子脂质体可用本领域熟知的方法从易购得的材料制得。例如参见,Szoka(1978)PNAS 75:4194-4198;WO90/11092关于DOTAP(1,2-二(油酰基氧)-3-(三甲基铵溶)丙烷)脂质体合成的描述。

同样,阴离子和中性脂质体也是容易获得的,例如购自Avanti PolarLipids(Birmingham,AL),或容易用易购得的材料制得。这种材料包括磷脂酰胆碱、胆固醇、磷脂酰乙醇胺、二油酰基磷脂酰胆碱(DOPC)、二油酰基磷脂酰甘油(DOPG)、二油酰基磷脂酰乙醇胺(DOPE)等。这些材料还能以合适比例与DOTMA和DOTAP原料混合。用这些材料制备脂质体的方法是本领域熟知的。

脂质体可包含多室运载体(MLV),小的单室运载体(SUV)、或大的单室运载体(LUV)。各种脂质体-核酸复合物可用本领域已知的方法制得。例如参见Straubinger(1983)Meth.Immunol.101:512-527;Szoka (1978)Proc.Natl.Acad.Sci.USA 75:4194-4198;Papahadjopoulos(1975)Biochim.Biophys.Acta 394:483;Wilson(1979)Cell 17:77);Deamer和Bangham(1976)Biochim.Biophys.Acta 443:629;Ostro(1977)Biochem.Biophys.Res.Commun.76:836;Fraley(1979)Proc.Natl.Acad.Sci.USA 76:3348);Enoch和Strittmatter(1979)Proc.Natl.Acad.Sci.USA 76:145;Fraley(1980)J.Biol.Chem.(1980)255:10431;Szoka和Papahadjopoulos(1978)Proc.Natl.Acad.Sci.USA 75:145;以及Schaefer-Ridder(1982)Science 215:166。

E.脂蛋白另外,脂蛋白也可加入待输送的多核苷酸或多肽中。采用的脂蛋白的例子包括:乳糜微粒、HDL、IDL、LDL和VLDL。还可采用这些蛋白的突变体、片段或融合蛋白。另外,可采用天然存在的脂蛋白的修饰物,例如乙酰化的LDL。这些脂蛋白能使多核苷酸的输送指向表达脂蛋白受体的细胞。较佳的,如果待输送的多核苷酸中加入了脂蛋白,则组合物中不加入其它寻靶的配体。

天然存在的脂蛋白包含脂质和蛋白部分。蛋白部分称为脱辅基蛋白。目前,已经分离并鉴定出了脱辅基蛋白A、B、C、D和E。其中至少有两个含有几种蛋白,用罗马数字AI、AII、AIV;CI、CII、CIII命名。

脂蛋白可包含多个脱辅基蛋白。例如,天然存在的乳糜微粒包含A、B、C和E,随着时间的推移,这些脂蛋白失去A,得到C和E脱辅基蛋白。VLDL包含A、B、C、和E脱辅基蛋白,LDL包含脱辅基蛋白B;HDL包含脱辅基蛋白A、C和E。

这些脱辅基蛋白的氨基酸序列是已知的,并且在下列文献中有所描述:Breslow(1985)Annu Rev.Biochem 54:699;Law(1986)Adv.Exp Med.Biol.151:162;Chen(1986)J Biol Chem 261:12918;Kane(1980)Proc Natl Acad Sci USA 77:2465;和Utermann(1984)Hum Genet 65:232。

脂蛋白含有各种脂质,包括甘油三酯、胆固醇(游离的和酯型)以及磷脂。天然存在的脂蛋白中脂质的组成是不同的。例如,乳糜微粒主要含甘油三酯。关于天然存在的脂蛋白的脂质含量更详细的描述可在例如Meth.Enzymol.128(1986)中找到。选择脂质的组成,以使脱辅基蛋白的构型有利于受体结合活性。还可选择脂质的组成,以促进与多核苷酸结合分子中的疏水性相互作用和结合。

天然存在的脂蛋白可以用诸如超离心方法从血清中分离出来。这些方法在Meth.Enzymol.(同上);Pitas(1980)J.BioChem.255:5454-5460以及Mahey(1979)J Clin.Invest64:743-750中有所描述。

脂蛋白还可在体外产生,或通过在所需宿主细胞中表达脱辅基蛋白基因的重组方法产生。例如参见Atkinson(1986)Annu Rev Biophys Chem 15:403和Radding(1958)Biochim Biophys Acta 30:443。

脂蛋白也可购自商品供应商,如Biomedical Techniologies,Inc.,Stoughton,Massachusetts,USA。

关于脂蛋白的进一步描述可在Zuckermann等人的PCT申请号No.US97/14465中找到。

F.聚阳离子试剂聚阳离子试剂可以与或不与脂蛋白一起包括在含所需待输送多核苷酸和/或多肽的组合物中。

聚阳离子试剂通常在生理性相关的pH下表现出净的正电荷,并能中和核酸的电荷,而有助于输送至所需位置。这些试剂具有体外、活体外和体内的用途。聚阳离子试剂可用来将核酸通过肌内或皮下等输送至活的对象。

下面是用作聚阳离子试剂的多肽例子:聚赖氨酸、聚精氨酸、聚鸟氨酸和鱼精蛋白。其它有用多肽的例子包括组蛋白、鱼精蛋白、人血清白蛋白、DNA结合蛋白、非组蛋白染色体蛋白、DNA病毒的外壳蛋白,如φX174,转录因子还含有结合DNA的结构域,因此可用作核酸凝聚剂。简言之,转录因子如C/CEBP、c-jun、c-fos、AP-1、AP-2、AP-3、CPF、Prot-1、Sp-1、Oct-1、Oct-2、CREP、和TFIID含有结合DNA序列的基本结构域。

聚阳离子有机试剂包括:精胺、亚精胺和腐胺。

从上面的清单可以推出聚阳离子试剂的尺寸和生理性能,以构建其它多肽聚阳离子试剂或产生合成的聚阳离子试剂。

G.合成的聚阳离子试剂可用于药物组合物中的合成聚阳离子试剂例如包括,DEAE-葡聚糖、Polybrene。LipofectinTM和lipofectAMINETM是与多核苷酸/多肽组合时形成聚阳离子复合物的单体。

免疫诊断试验本发明的奈瑟球菌MenB抗原或其抗原性片段,可用于免疫试验来检测抗体水平(或相反,可用抗奈瑟球菌抗体来检测抗原水平)。根据明确的免疫试验,可以开发重组抗原,以代替侵入性诊断方法。可检测生物学样品(例如包括血液或血清样品)中的抗奈瑟球菌MenB蛋白或其片段的抗体。免疫试验的设计可作很大变化,其各种方案均是本领域中已知的。免疫试验的方案可基于例如竞争性、或直接反应或夹心型试验。方案例如还可采用固体支持物,或可以采用免疫沉淀法。大多数试验涉及采用标记的抗体或多肽;该标记例如可以是荧光标记、化学发光标记、放射活性标记或染料分子。扩增探针信号的试验也是已知的;其例子是采用生物素和亲和素的试验,酶标记的和介导的免疫试验,如ELISA试验。

将合适的材料(包括本发明的组合物)以及进行试验所需的其它试剂和材料(例如合适的缓冲液、盐溶液等)和合适的试验说明书包装到合适的容器中,构成适用于免疫诊断且含有适当标记的试剂的试剂盒。

核酸杂交“杂交”指两个核酸序列相互之间通过氢键而结合。通常,一个序列固定于固体载体,另一个将游离于溶液内。然后,在有利于形成氢键的条件下使两个序列相互接触。影响这种结合的因素包括:溶剂的类型和体积;反应温度;杂交时间;搅拌;封闭液相序列与固体载体非特异性结合的试剂(Denhardt′s试剂或BLOTTO);各序列的浓度;是否使用化合物来增加序列结合的速度(硫酸葡聚糖或聚乙二醇);以及杂交后洗涤条件的严谨程度。见Sambrook等人[同上]第2卷,第9章,9.47至9.57页。

“严谨性”指有利于非常相似的序列结合而不利于不同序列结合的杂交反应条件。例如,应选择温度和盐浓度的组合,使温度比所研究的杂交的Tm计算值低大约120至200℃。温度和盐浓度常可通过初步实验凭经验来确定,在初步实验中,固定在滤膜上的基因组DNA样品与感兴趣的序列杂交,然后在不同的严谨度条件下洗涤。见Sambrook等人第9.50页。

在进行例如Southern印迹时,要考虑的参数是(1)待印迹的DNA的复杂性以及(2)探针与受检测序列之间的同源性。对于高度复杂的真核基因组中的单拷贝基因,待研究片段的总量可以在10的一个数量级范围内变化,质粒为0.1至1微克,或将噬菌体消化至10-9至10-8克。对于复杂性较低的多核苷酸,可以采用实际上更短的印迹、杂交以及接触时间,更少量的起始多核苷酸,以及比活更低的探针。例如,从1微克酵母DNA开始,用仅仅1小时的接触时间,印迹2小时,然后和108cpm/μg的探针杂交4-8小时,就可以检测单拷贝酵母基因。对于单拷贝哺乳动物基因而言,一种保守的方法是从10微克DNA开始,印迹过夜,在10%硫酸葡聚糖存在下用108cpm/μg以上的探针杂交过夜,导致接触时间约为24小时。

有几个因素可能会影响探针与感兴趣片段之间的DNA-DNA杂交物的解链温度(Tm),因而影响杂交和洗涤的合适条件。在许多情况下,探针并非与片段100%同源。其它常常遇到的变量包括杂交序列的长度和G+C总含量,以及杂交缓冲液的离子强度和甲酰胺含量。所有这些因素的作用可近似表示成一个方程式:      Tm=81+16.6(log10Ci)+0.4[%(G+C)]-0.6(%甲酰胺)-600/n-1.5(%错配)其中Ci是盐浓度(单价离子),n是杂交物碱基对的长度(对Meinkoth和Wahl(1984)Anal.Biochem.138:267-284中的稍稍作了修改)。

在设计杂交实验时,影响核酸杂交的一些因素可以方便地予以改变。杂交和洗涤时的温度以及洗涤时的盐浓度的调节最为简单。随着杂交温度(即严谨度)的升高,不同源的链之间发生杂交的可能性变得更少,结果背景值降低。如果放射性标记的探针并非与固定的片段完全同源(这在基因家族和种间杂交实验中是常见的),则必须降低杂交温度,而背景值将会增加。洗涤温度以类似的方式影响杂交带的强度和背景值的程度。洗涤的严谨性也随盐浓度的降低而升高。

通常,在50%甲酰胺存在下的方便的杂交温度是:对于靶片段同源性达95%至100%的探针而言,是42℃;对于同源性为90%至95%的探针,为37℃;对于同源性为85%和90%的探针,为32℃。对于较低的同源性,应用上述方程式应相应地降低甲酰胺含量和调节温度。如果探针和靶片段之间的同源性是未知的,则最简单的方法是从非严谨的杂交和洗涤条件开始。如果在放射自显影后发现了非特异性的条带或高背景值,则可在高严谨性下洗涤滤膜,并重新曝光。如果曝光所需时间使得该方法不切实际,则应平行测试几种杂交和/或洗涤严谨性。

核酸探针试验采用本发明的核酸探针的方法(如PCR、分支DNA探针试验或印迹技术)能确定cDNA或mRNA的存在。如果探针和本发明的序列能形成稳定地足以被检测到的双链体或双链复合物,则称探针与本发明的序列“杂交”。

核酸探针将与本发明的奈瑟球菌核苷酸序列(包括有义和反义链)杂交。尽管有许多不同的核苷酸序列编码该氨基酸序列,但是天然的奈瑟球菌序列是较佳的,因为它是实际存在于细胞中的序列。mRNA代表一种编码序列,因此探针应与该编码序列互补;单链cDNA与mRNA互补,因此cDNA探针应与非编码序列互补。

探针序列无需和奈瑟球菌序列(或其互补体)相同,序列以及长度的一些差异能增加试验的灵敏度,如果核酸探针能和靶核苷酸形成能被检测的双链体的话。另外,核酸探针可包括其它核苷酸,以使形成的双链体稳定。其它奈瑟球菌序列也是有帮助的,可作为检测形成的双链体的标记。例如,非互补的核苷酸序列可以和探针的5′端相连,探针序列的其余部分与奈瑟球菌序列互补。或者,可将非互补的碱基或较长的序列散布到探针中,只要探针序列与奈瑟球菌序列有足够的互补性以便与其杂交从而形成能被检测的双链体。

探针的确切长度和序列将取决于杂交条件,如温度、盐浓度等。例如,对于诊断应用,根据分析物序列的复杂程度,核酸探针通常含有至少10-20个核苷酸,较佳的15-25个,更佳的至少30个核苷酸,但是也可短于该长度。短的引物通常需要较低温度,以便和模板形成足够稳定的杂交复合物。

探针可用合成方法产生,例如Matteucci等人[J.Am.Chem.Soc.(1981)103:3185]的方法或Urdea等人[Proc.Natl.Acad.Sci.USA(1983)80:7461]的方法,或用市售的自动寡核苷酸合成仪合成。

可以根据偏好选择探针的化学特征。对于某些应用,DNA或RNA是合适的。对于其它的应用,可以加入修饰,例如骨架修饰,如硫代磷酸酯或甲基磷酸酯,可用来增加体内半衰期,改变RNA亲和力,增加核酸酶抗性等[例如参见Agrawal和Iyer(1995)Curr Opin Biotechnol 6:12-19;Agrawal(1996)TIBTECH 14:376-387];还可采用类似物如肽核酸[例如参见Corey(1997)TIBTECH 15:224-229;Buchardt等人(1993)TIBTECH11:384-386]。

一种核苷酸杂交试验的例子由Urdea等人描述于国际专利申请WO 92/02526[还可参见美国专利5,124,246]。

另外,聚合酶链反应(PCR)是另一个熟知的检测少量靶核酸的手段。该试验在Mullis等人[Meth.Enzymol.(1987)155:335-350];美国专利4,683,195和4,683,202中有所描述。用两个“引物”核苷酸与靶核酸杂交,并用来引导反应。引物可包含不与扩增靶序列(或其互补序列)杂交的序列,以帮助双链体的稳定性,或例如可插入一个简便的限制性位点。这些序列通常侧接所需的奈瑟球菌序列。

利用最初的靶核酸作为模板,热稳定的聚合酶能从引物产生靶核酸的拷贝。在聚合酶产生临界量的靶核酸后,它们可用较传统的方法(如Southern印迹)来检测。当采用Southern印迹方法时,标记的探针将与奈瑟球菌序列(如其互补序列)杂交。

另外,mRNA或cDNA也可用Sambrook等人[同上]描述的传统印迹技术来检测。用凝胶电泳可纯化并分离利用聚合酶从mRNA产生的mRNA或cDNA。然后,将凝胶上的核酸印迹到固体载体如硝酸纤维素上。使固体载体与标记的探针接触,然后洗涤除去所有未杂交的探针。然后,检测含有标记探针的双链体。该探针通常用放射活性物质作标记。

实施例本发明基于来自脑膜炎奈瑟球菌的′573专利申请的附录C中的SEQ ID NO:1-961中所示的961个核苷酸序列(这961个序列合起来基本上代表了脑膜炎奈瑟球菌B血清型全基因组),′573专利申请的附录D中SEQ ID NO:1068所示的全长基因组序列,以及本申请附录A中SEQ ID NO:1所示的全长基因组序列。

如上所述,对于技术人员而言如何利用本发明的这种序列信息是不言而喻的。

可用于实施本发明(例如将所公开的序列用于预测用于疫苗或诊断目的的多肽)的标准技术和程序,在上文中已作概述。该概述不是对本发明的限制,相反给出了可以使用但并不是必需使用的例子。

这些序列来自附录C中所示的毗连群(SEQ ID NO:1-961)和附录D中所示的全长基因组序列(SEQ ID NO:1068),它们是在对脑膜炎奈瑟球菌(B菌株)的基因组进行测序过程中制备的。全长序列是通过使用TIGR Assembler软件[G.S.Sutton等人,TIGRAssembler:A New Tool for Assembling Large Shotgun Sequencing Projects,GenomeScience and Technology,1:9-19(1995)],[还可参见R.D.Fleischmann,等人,Science 269,496-512(1995);C.M.Fraser,等人,Science 270,397-403(1995);C.J.Bult,等人,Science 273,1058-73(1996);C.M.Fraser,等人,Nature 390,580-586(1997);J.-F.Tomb,等人.,Nature 388,539-547(1997);H.P.Klenk,等人,Nature 390,364-70(1997);C.M.Fraser,等人,Science 281,375-88(1998);M.J.Gardner,等人,Science 282,1126-1132(1998);K.E.Nelson,等人,Nature 399,323-9(1999)]而装配在一起的。然后,使用上述方法,确定推定的序列翻译产物。翻译产物的计算机分析可根据数据库比较加以确定。在脑膜炎奈瑟球菌(A菌株)和淋病奈瑟球菌中鉴别出相应的基因和蛋白质序列(如果存在)。接着,表达、纯化蛋白质,并进行表征以评估其抗原性和免疫原性。

具体地说,采用下列方法来表达、纯化和分析本发明蛋白的生物化学特性。

染色体DNA制备使脑膜炎奈瑟球菌2996菌株在100毫升GC培养基中生长至指数期,离心收获,重悬于5毫升缓冲液(20%蔗糖、50毫摩尔Tris-HCl、50毫摩尔EDTA、调至pH 8)中。冰上培育10分钟后,加入10毫升裂解溶液(50毫摩尔NaCl,1%十二烷基肌氨酸钠,50微克/毫升蛋白酶K)裂解该细菌,37℃培育悬液2小时。用苯酚抽提两次(平衡至pH8),用三氯甲烷/异戊醇(24∶1)抽提一次。加入0.3M乙酸钠和2体积乙醇,使DNA沉淀,离心收集。用70%乙醇洗涤沉淀一次,重新溶解在4.0毫升TE缓冲液(10毫摩尔Tris-HCl,1毫摩尔EDTA,pH 8)中。通过读取260纳米处的OD值而测定DNA浓度。

寡核苷酸设计用(a)脑膜炎球菌B的序列(当能获得时),或(b)淋球菌/脑膜炎球菌A的序列(根据脑膜炎球菌密码子偏好利用率进行调整),根据各ORF的编码序列,设计合成的寡核苷酸引物。推导针对紧靠预计前导序列的下游序列的5′端引物,从而忽略预计的信号肽。

对于大多数ORF,5′引物包括两个限制性酶识别位点(BamHI-NdeI,BamHI-NheI、EcoRI-NdeI,这取决于基因的限制图谱)。3′引物包括XhoI限制性位点。建立该步骤是为了指导将各扩增产物(对应于各ORF)克隆到以下两个不同的表达系统中:pGEX-KG(用BamHI-XhoI或EcoRI-XhoI),以及pET21b+(用NdeI-XhoI或NheI-XhoI)。

5′-端引物尾序列:CGCGGATCCCATATG    (BamHI-NdeI)                  CGCGGATCCGCTAGC    (BamHI-NheI)                  CCGGAATTCTAGCTAGC (EcoRI-NheI)3′-端引物尾序列:CCCGCTCGAG         (XhoI)对于某些ORF,可进行2种不同的扩增,以便将每个ORF克隆在两种表达系统中。对每个ORF使用两种不同的5′引物;使用如前所述相同的3′XhoI引物:5′-端引物尾序列:GGAATTCCATATGGCCATGG  (NdeI)5′-端引物尾序列:CGGGATCC              (BamHI)其他ORF被克隆在pTRC表达载体中,并以氨基端His-tag融合蛋白形式表达。在最终产物中包含预计的信号肽。用下列引物引入NheI-BamHI限制性位点:5′-端引物尾序列:GATCAGCTAGCCATATG(NheI)3′-端引物尾序列:CGGGATCC         (BamHI)引物不仅含有限制性酶识别序列,而且还包括能与待扩增序列杂交的核苷酸。杂交的核苷酸数目取决于整个引物的解链温度,并且对于各引物可用下式确定:Tm=4(G+C)+2(A+T)                        (排除尾部)Tm=64.9+0.41(%GC)-600/N                (整个引物)对于整个寡核苷酸来说,所选寡核苷酸的平均解链温度为65-70℃,对于单杂交区来说,解链温度为50-55℃。

用Perkin Elmer 394DNA/RNA合成仪合成寡核苷酸,用2.0毫升NH4-OH从柱上洗脱下,56℃培育5小时去保护。加入0.3M乙酸钠和2体积乙醇,使寡核苷酸沉淀。然后离心样品,将沉淀重悬于100微升或1毫升水中。用Perkin Elmer λ Bio分光光度计测定OD260,测得浓度,调节至2-10pmol/微升。

表1显示了用于各扩增反应的正向和反向引物。在某些情况下,应注意引物序列并不完全与ORF的序列精确匹配。在进行最初的扩增时,并不知道一些脑膜炎球菌的ORF的完整5′和/或3′序列,尽管已经鉴定出了淋球菌中的对应序列。对于扩增反应,可使用淋球菌的序列作为引物设计的基础,并根据密码子偏好性对这些序列作了改动。具体地,可改变下列密码子:ATA→ATT;TCG→TCT;CAG→CAA;AAG→AAA;GAG→GAA;CGA和CGG→CGC;GGG→GGC。

扩增标准的PCR程序如下:在20-40微摩尔各寡核苷酸、400-800微摩尔dNTP溶液、1x PCR缓冲液(包括1.5毫摩尔氯化镁)、2.5单位TaqI DNA聚合酶(用Perkin-ElmerAmpliTaQ,GIBCO Platinum,Pwo DNA聚合酶或Tahara Shuzo Taq聚合酶)存在下,用50-200ng基因组DNA作为模板。

在一些例子中,通过加入10微升DMSO或50微升2M甜菜碱来优化PCR。

在加热开始后(95℃初步培育整个混合物3分钟期间加入聚合酶),每个样品经历两步扩增:前5个循环的进行使用排除限制性酶尾部后一个寡核苷酸的杂交温度。随后进行的30循环,使用全长寡核苷酸的杂交温度。这些循环之后是在72℃最后延伸10分钟。

标准循环如下:

延伸时间随待扩增ORF的长度而不同。

扩增用9600或2400 Perkin Elmer GeneAmp PCR系统进行。为了检查结果,将1/10的扩增体积加到1-1.5%琼脂糖凝胶上,将各扩增片段的大小与DNA分子量标记作比较。

将扩增的DNA直接上样到1%琼脂糖凝胶上,或是先用乙醇沉淀,然后重悬于合适的体积中,上样到1%琼脂糖凝胶上。然后用Qiagen凝胶抽提试剂盒,按照生产商说明,从凝胶中洗脱和纯化出对应于大小正确条带的DNA片段。DNA片段的最终体积是30微升或50微升,在水或10mM Tris,pH 8.5中。

PCR片段的消化将对应于扩增片段的纯化DNA分成2等份,用下列限制酶进行双重消化:NdeI/XhoI或NheI/XhoI,以便克隆入pET-21b+并进一步以C-末端His尾的融合蛋白形式表达蛋白;BamHI/XhoI或EcoRI/XhoI,克隆入pGEX-KG并以N末端GST融合形式进一步表达蛋白;对于ORF 76,用NheI/BamHI消化,克隆入pTRC-HisA载体并以N末端His尾融合蛋白形式进一步表达蛋白。

在合适的消化缓冲液存在下,使各纯化的DNA片段与20单位的各限制性酶(NewEngland Biolabs)在30或40微升终体积中37℃培育3小时至过夜。然后用QIAquick PCR纯化试剂盒,按照生产商说明书纯化消化产物,以30微升或50微升终体积用水或10mMTris-HCl(pH 8.5)洗脱。在已标定的分子量标记存在下,通过1%琼脂糖凝胶电泳测定DNA终浓度。

克隆载体(pET22B,pGEX-KG,和pTRC-His A)的消化在合适的缓冲液存在下,在200微升反应体积中用50单位的各限制性酶对10微克质粒进行双消化37℃培育过夜。将全部消化物上样到1%琼脂糖凝胶上后,用QiagenQIAquick凝胶抽提试剂盒从凝胶中纯化对应于消化载体的条带,将DNA洗脱到50微升10毫摩尔Tris-HCl,pH 8.5中。测定样品的OD260评价其DNA浓度,并将浓度调节至50微克/微升。每个克隆步骤使用1微升质粒。

克隆将预先消化和纯化的、对应于各ORF的片段连接到pET22b和pGEX-KG中。在20微升的最终体积,在生产商提供的缓冲液存在下,用0.5微升NEB T4 DNA连接酶(400单位/微升)连接摩尔比为3∶1的片段/载体。室温培育反应3小时。在一些实验中,用Boheringer的″快速连接试剂盒″按照生产商说明书进行连接。

为了将重组质粒导入合适的菌株内,使100微升大肠杆菌DH5感受态细胞与连接酶反应溶液于冰上培育40分钟,然后37℃3分钟,然后加入800微升LB肉汤后,再37℃培育20分钟。然后在Eppendorf微量离心机(microfuge)中以最大速度离心细胞,重悬于约200微升上清液中。然后将悬液接种到LB氨苄青霉素(100毫克/毫升)平板上。

使5个随机选择的菌落在2毫升(pGEX或pTC克隆)或5毫升(pET克隆)LB肉汤+100微克/毫升氨苄青霉素中37℃生长过夜,对重组克隆进行筛选。然后,使细胞沉淀,用Qiagen QIAprep旋转微量制备试剂盒,按照生产商说明书,将DNA抽提到最终体积为30微升。用NdeI/XhoI或BamHI/XhoI消化5微升各个微量制备物(约1微克),将整个消化物上样到1-1.5%琼脂糖凝胶上(取决于预计的插入片段大小),与分子量标记(1KbDNA梯序列,GIBCO)平行。根据正确的插入片段大小筛选阳性克隆。

克隆通过使用EcoRI-PstI、或EcoRI-SalI、或SalI-PstI克隆位点,将某些ORF克隆入pGEX-His载体中。克隆后,可将重组质粒导入大肠杆菌W3110宿主中。

表达然后,将克隆到表达载体中的每个ORF转化入适合表达重组蛋白产物的菌株中。用1微升各构建物转化上述30微升大肠杆菌BL21(pGEX载体)、大肠杆菌TOP10(pTRC载体)或大肠杆菌BL21-DE3(pET载体)。在pGEX-His载体例子中,用相同的大肠杆菌菌株(W3110)进行最初的克隆和表达。将单个重组菌落接种到2毫升LB+Amp(100微克/毫升)中,37℃培育过夜,然后1∶30稀释在100毫升瓶中的20毫升LB+Amp(100微克/毫升)中,确保OD600在0.1至0.15之间。将瓶培育在30℃的回转水浴摇床中,直至OD表明达到适合诱导表达的指数生长(pET和pTRC载体的OD为0.4-0.8;pGEX和pGEX-His载体的OD为0.8-1)。对于pET,pTRC和pGEX-His载体,加入1毫摩尔IPTG,诱导蛋白质表达,而在pGEX系统情况下,IPTG的最终浓度为0.2毫摩尔。30℃培育3小时后,测OD检查样品的最终浓度。为了检查表达,取出各样品1毫升,在微量离心机中离心,将沉淀重悬于PBS中,用12%SDS-PAGE和考马斯蓝染色分析。6000g离心整个样品,将沉淀重悬于PBS中待用。

GST-融合蛋白的大规模纯化使单菌落在LB+Amp琼脂板上37℃培育过夜。将细菌接种到20毫升LB+Amp培养液中,水浴摇床上生长过夜。将细菌1∶30稀释到600毫升新鲜培养基中,使其在最适温度(20-37℃)下生长至OD550为0.8-1。用0.2毫摩尔IPTG诱导蛋白质表达,培育3小时。4℃、8000rpm离心培养物。弃去上清液,将细菌沉淀重悬于7.5毫升冷的PBS中。用40W的Brason超声波仪B-15在冰上超声破碎细胞30秒种,冻融2次,再次离心。收集上清液,与150微升谷胱苷肽-Sepharose 4B树脂(Pharmacia)(先用PBS洗涤)混合,室温下培育30分钟。4℃、700g离心样品5分钟。用10毫升冷的PBS洗涤树脂2次10分钟,重悬于1毫升冷的PBS中,上样于一次性柱中。用2毫升冷PBS洗柱2次,直至流穿液OD280达到0.02-0.06。加入700微升冷的谷胱苷肽洗脱缓冲液(10毫摩尔还原的谷胱苷肽,50毫摩尔Tris-HCl),洗脱GST-融合蛋白,收集各组分直至OD280为0.1。将各组分21微升上样于12%SDS凝胶上,凝胶采用Biorad SDS-PAGE分子量标准宽范围(M1)(200,116.25,97.4,66.2,45,31,21.5,14.4,6.5kDa)或AmershamRainbow标记(M″)(220,66,46,30,21.5,14.3kDa)作为标准。因为GST的MW为26kDa,因此该值必须加到各GST-融合蛋白的MW中。

可溶性His-融合蛋白的大规模纯化使单菌落在LB+Amp琼脂板上37℃培育过夜。将细菌接种到20毫升LB+Amp培养液中,并在水浴摇床上生长过夜。将细菌1∶30稀释到600毫升新鲜培养基中,使其在最适温度(20-37℃)下生长至OD550为0.6-0.8。加入1毫摩尔IPTG诱导蛋白质表达,培养物再培育3小时。4℃8000rpm离心培养物。弃去上清液,将细菌沉淀重悬于7.5毫升冷的10mM咪唑缓冲液中(300mM Nacl,50mM磷酸缓冲液,10mM咪唑pH8)。用40W的Brason超声波仪B-15在冰上超声破碎细胞30秒种,冻融2次,然后再次离心。收集上清液,与150微升Ni2+-树脂(Pharmacia)(预先用10mM咪唑缓冲液洗涤)混合,室温轻柔搅拌培育30分钟。4℃,700g离心样品5分钟。树脂用10毫升冷的10mM咪唑缓冲液洗涤二次,各10分钟,然后重悬于1毫升冷的10mM咪唑缓冲液中,上样于一次性柱中。4℃,用2毫升冷的10mM咪唑缓冲液洗涤树脂,直至流穿液OD280达到0.02-0.06。用2毫升冷的20mM咪唑缓冲液(300mM NaCl,50mM磷酸盐缓冲液,20mM咪唑,pH 8)洗涤树脂,直至流穿液OD280达到0.02-0.06。加入700微升冷的250mM咪唑缓冲液(300mM NaCl,50mM磷酸盐缓冲液,250mM咪唑,pH 8),洗脱His-融合蛋白,并收集各组分直至OD280为0.1。将各组分21微升上样于12%SDS凝胶上。

不溶性His融合蛋白大规模纯化使单菌落在LB+Amp琼脂板上37℃培育过夜。将细菌接种到20毫升LB+Amp培养液中,在水浴摇床中培育过夜。将细菌1∶30稀释到600毫升新鲜培养基中,使其在最适温度(37℃)下生长至OD550为0.6-0.8。加入1毫摩尔IPTG诱导蛋白质表达,继续培育该培养物3小时。4℃、8000rpm离心培养物,弃去上清液,将细菌沉淀重悬于7.5毫升缓冲液B中(尿素8M,10毫摩尔Tris-HCl,100毫摩尔磷酸缓冲液,pH 8.8)。用Brason超声波仪B-15于40W在冰上超声破碎细胞30秒种,冻融2次,然后再次离心。上清液保存在-20℃,而沉淀重悬于2毫升胍缓冲液(6M盐酸胍,100毫摩尔磷酸缓冲液,10毫摩尔Tris-HCl,pH 7.5)中,在匀化器中处理10个循环。13000rpm离心产物40分钟。将上清液与150微升Ni2+-树脂(Pharmacia)(先用缓冲液B洗涤)混合,并在室温下轻微搅动培育30分钟。样品4℃,700g离心5分钟。树脂用10毫升缓冲液B洗涤二次10分钟,然后重悬于1毫升缓冲液B中,上样于一次性柱中。树脂用2毫升缓冲液B室温洗涤,直至流穿液OD280达到0.02-0.06。树脂用2毫升缓冲液C(尿素8M,10毫摩尔Tris-HCl,100毫摩尔磷酸缓冲液,pH 6.3)洗涤,直至流穿液OD280达到0.02-0.06。加入700微升洗脱缓冲液(尿素8M,10毫摩尔Tris-HCl,100毫摩尔磷酸缓冲液,pH 4.5)洗脱His-融合蛋白,收集各组分直至OD280为0.1。将各组分的21微升上样于12%SDS凝胶。

His-融合蛋白复性在变性的蛋白中加入10%甘油。然后用透析缓冲液I(10%甘油,0.5M精氨酸,50毫摩尔磷酸缓冲液,5毫摩尔还原的谷胱苷肽,0.5毫摩尔氧化的谷胱苷肽,2M尿素,pH 8.8)将蛋白质稀释至20微克/毫升,用相同的缓冲液4℃透析12-14小时。用透析缓冲液II(10%甘油,0.5M精氨酸,50mM磷酸缓冲液,5毫摩尔还原的谷胱苷肽,0.5毫摩尔氧化的谷胱苷肽,pH 8.8)进一步4℃透析蛋白质12-14小时。

用下式评价蛋白浓度:蛋白质(毫克/毫升)=(1.55×OD280)-(0.76×OD260)小鼠免疫用各纯化蛋白20微克腹膜内免疫小鼠。对于某些ORF,用氢氧化铝作为佐剂,在第1、21和42天免疫Balb-C小鼠,检测第56天所取样品中的免疫应答。对于其他ORF,可用相同方案免疫CD1小鼠。对于其他ORF,可用Freund佐剂免疫CD1小鼠,采用相同的免疫方案,只是在第42天而非56天测定免疫应答。同样,对于另一些ORF,可用Freund佐剂免疫CD1小鼠,但是在第49天测定免疫应答。

ELISA试验(血清分析)将无荚膜MenB M7菌株接种到巧克力琼脂板上,37℃培育过夜。用无菌挑菌拭子收集琼脂板的细菌菌落,接种到7毫升含0.25%葡萄糖的Mueller-Hinton肉汤(Difco)中。跟踪OD260每30分钟监测细菌生长。使细菌长至OD达到0.3-0.4。10000rpm离心培养物10分钟。弃去上清液,用PBS洗涤细菌1次,重悬于含0.025%甲醛的PBS中,室温培育2小时,然后4℃搅拌过夜。在96孔Greiner板的每个孔中加入100微升细菌细胞,4℃培育过夜。然后用PBT洗涤缓冲液(0.1%吐温-20,PBS配)洗涤孔三次。每孔中加入200微升饱和缓冲液(含2.7%聚乙烯吡咯烷酮10的水),37℃培育平板2小时。用PBT洗涤各孔3次。每孔中加入200微升稀释的血清(稀释缓冲液:1%BSA,0.1%吐温-20,0.1%叠氮钠,PBS配),37℃培育平板90分钟。用PBT洗孔三次。每孔中加入100微升以稀释缓冲液1∶2000稀释的HRP-偶联的家兔抗小鼠(Dako)血清,37℃培育平板90分钟。用PBT缓冲液洗涤孔三次。每孔中加入100微升HRP的底物缓冲液(25毫升柠檬酸缓冲液pH 5,10毫克邻苯二胺和10微升双氧水),平板室温放置20分钟。每孔中加入100微升硫酸,并随后测OD490。当OD490为各自免疫前血清OD值的2.5倍时,认为ELISA呈阳性。

FACScan细菌结合试验程序将无荚膜MenB M7菌株接种到巧克力琼脂板上,37℃培育过夜。用无菌挑菌拭子收集琼脂板上的细菌菌落,接种到各含8毫升0.25%葡萄糖的Mueller-Hinton肉汤(Difco)的4个试管中。跟踪OD620,每30分钟监测细菌生长。使细菌长至OD值达到0.35-0.5。4000rpm离心培养物10分钟。弃去上清液,将沉淀重悬于封闭缓冲液(1%BSA,0.4%叠氮钠)中,4000rpm离心5分钟。将细胞重悬于封闭缓冲液中,至OD620为0.07。在Costar 96孔板的每孔中加入100微升细菌细胞。在每孔中加入100微升稀释的(1∶200)血清(封闭缓冲液配),4℃培育平板2小时。4000rpm离心细胞5分钟,吸出上清液,每个孔中加入200微升封闭缓冲液,洗涤细胞。在每个孔中加入1∶100稀释的R-Phicoerytrin偶联的F(ab)2山羊抗小鼠抗体,4℃培育平板1小时。4000rpm离心5分钟,使细胞旋转沉淀,在每个孔中加入200微升封闭缓冲液进行洗涤。吸出上清液,将细胞重悬于每孔200微升PBS和0.25%甲醛中。将样品转移到FACScan管中读数。FACScan设置条件为:FL1开启,FL2和FL3关闭;FSC-H临界值:92;FSC PMT电压:E 02;SSC PMT:474;Amp.Gains 7.1;FL-2PMT:539;补偿值:0。

OMV制备使细菌在5GC平板上生长过夜,用挑菌环收获,重悬于10毫升20毫摩尔Tris-HCl中。56℃热灭活30分钟,在冰上超声破碎该细菌10分钟(50%负载循环,50%输出)。5000g离心10分钟,除去未破碎的细胞,4℃、50000g离心75分钟,回收全部细胞包膜组分。为了从粗制的外膜中抽提出细胞质膜蛋白,将全部组分重悬于2%十二烷基肌氨酸钠(Sigma)中,室温培育20分钟。10000g离心该悬浮液10分钟,除去凝聚物,对上清液进一步50000g超离心75分钟,使外膜沉淀。将外膜重悬于10毫摩尔Tris-HCl,pH 8中,用BioRad蛋白质试验以BSA为标准品测定蛋白浓度。

全抽提物制备使细菌在GC板上生长过夜,用挑菌环收获,重悬于1毫升20毫摩尔Tris-HCl中。56℃热灭活30分钟。

Western印迹将MenB菌株2996的纯化蛋白(每条泳道500ng)、外膜囊泡(5微克)和全细胞抽提物(25微克)上样于15%SDS-聚丙烯酰胺凝胶,然后转移到硝酸纤维素膜上。转移在转移缓冲液(0.3%Tris碱,1.44%甘氨酸,20%甲醇)中,在4℃和150mA下进行2小时。在饱和缓冲液(10%脱脂乳、0.1%Triton X100,PBS配)中4℃培育过夜,使该膜饱和。用洗涤缓冲液(3%脱脂乳,0.1%Triton X 100,PBS配)洗涤该膜两次,并与洗涤缓冲液1∶200稀释的小鼠血清37℃培育2小时。洗涤该膜两次,和稀释度为1∶2000的辣根过氧化物酶标记的抗小鼠Ig培育90分钟。用含0.1%Triton X100的PBS洗涤该膜两次,用Opti-4CN底物试剂盒(Bio-Rad)显影。加入水,终止反应。

杀菌试验使MC58菌株在巧克力琼脂板上37℃生长过夜。收集5-7个菌落,用于接种7毫升Mueller-Hinton肉汤。在震摇器上37℃培育该悬浮液,使其生长,至OD620为0.5-0.8。将培养液等分到1.5毫升无菌Eppendorf管中,在微量离心机中以最大速度离心20分钟。以Gey′s缓冲液(Gibco)洗涤沉淀一次,重悬于相同缓冲液中,至OD620为0.5,以Gey′s缓冲液1∶20000稀释,25℃保藏。

在96孔组织培养板的每孔中加入50微升Gey′s缓冲液/1%BSA。在每个孔中加入25微升稀释(1∶100)的小鼠血清(稀释缓冲液:Gey′s缓冲液/0.2%BSA中),4℃培育平板。将25微升前述细菌悬浮液加入每个孔中。每个孔中加入25微升热灭活(56℃水浴30分钟)的或正常的幼兔补体。在加入幼兔补体后,立即将每个孔中22微升的样品接种到Mueller-Hinton琼脂板(时间0)上。37℃转动培育96孔板1小时,然后将每个孔内22微升的样品接种到Mueller-Hinton琼脂板(时间1)上。过夜培育后,计数对应于时间0和时间1小时的菌落数。

下列DNA和氨基酸序列用下列形式的标题加以限定:[g,m,或a][#].[seq或pep],其中“g”表示序列来自淋病奈瑟球菌,“m”表示序列来自脑膜炎奈瑟球菌B,而“a”表示序列来自脑膜炎奈瑟球菌A;“#”表示序列编号;“seq”表示DNA序列,而“pep”表示氨基酸序列。例如,“g001.seq”指编号为1的淋病奈瑟球菌DNA序列。当这些序列存在后缀“-1”或“-2”时,表示对于同一ORF发现的额外序列。此外,开放阅读框以“ORF#”表示,其中“#”指ORF的编号,对应于编码ORF的序列的编号。此外,ORF命名可有后缀“.ng”或“.a”,分别表示该ORF对应于淋病奈瑟球菌序列或脑膜炎奈瑟球菌A序列。用计算机分析了“g”、“m”和“a”肽序列之间的比较。在本文中,“pep”后缀暗示那里没有明确地说明。

实施例1下列ORF是用本文所述方法,从毗连群序列和/或全长序列中预测出的。

ORF的定位ORF:毗连群:279 gnm4.seq下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 2>:m279.seq  1 ATAACGCGGA TTTGCGGCTG CTTGATTTCA ACGGTTTTCA GGGCTTCGGC 51 AAGTTTGTCG GCGGCGGGTT TCATCAGGCT GCAATGGGAA GGTACGGACA101 CGGGCAGCGG CAGGGCGCGT TTGGCACCGG CTTCTTTGGC GGCAGCCATG151 GCGCGTCCGA CGGCGGCGGC GTTGCCTGCA ATCACGATTT GTCCGGGTGA201 GTTGAAGTTG ACGGCTTCGA CCACTTCGCT TTGGGCGGCT TCGGCACAAA251 TGGCTTTAAC CTGCTCATCT TCCAAGCCGA GAATCGCCGC CATTGCGCCC301 ACGCCTTGCG GTACGGCGGA CTGCATCAGT TCGGCGCGCA GGCGCACGAG351 TTTGACCGCG TCGGCAAAAT TCAATGCGCC GGCGGCAACG AGTGCGGTGT401 ATTCGCCGAG GCTGTGTCCG GCAACGGCGG CAGGCGTTTT GCCGCCCGCT451 TCTAAATAG这对应于氨基酸序列<SEQ ID 3;ORF 279>:

m279.pep  1  ITRICGCLIS TVFRASASLS AAGFIRLQWE GTDTGSGRAR LAPASLAAAM 51 ARPTAAALPA ITICPGELKL TASTTSLWAA SAQMALTCSS SKPRIAAIAP101 TPCGTADCIS SARRRTSLTA SAKFNAPAAT SAVYSPRLCP ATAAGVLPPA151 SK*下列部分DNA序列是在淋病奈瑟球菌中鉴别出的<SEQ ID 4>:g279.seq  1 atgacgcgga tttgcggctg cttgatttca acggttttga gtgtttcggc 51 aagtttgtcg gcggcgggtt tcatcaggct gcaatgggaa ggaacggata101 ccggcagcgg cagggcgcgt ttggctccgg cttctttggc ggcagccatg151 gtgcgtccga cggcggcggc gttgcctgca atcacgactt gtccgggcga201 gttgaagttg acggcttcga ccacttcgcc ctgtgcggat tcggcacaaa251 tctgcctgac ctgttcatct tccaaaccca aaatggccgc cattgcgcct301 acgccttgcg gtacggcgga ctgcatcagt tcggcgcgca ggcggacgag351 tttgacggca tcggcaaaat ccaatgcttc ggcggcgaca agcgcggtgt401 attcgccgag gctgtgtccg gcaacggcgg caggcgtttt gccgcccact451 tccaaatag这对应于氨基酸序列<SEQ ID 5;ORF 279.ng>:g279.pep  1 MTRICGCLIS TVLSVSASLS AAGFIRLQWE GTDTGSGRAR LAPASLAAAM 51 VRPTAAALPA ITTCPGELKL TASTTSPCAD SAQICLTCSS SKPKMAAIAP101 TPCGTADCIS SARRRTSLTA SAKSNASAAT SAVYSPRLCP ATAAGVLPPT151 SK*ORF 279与淋病奈瑟球菌中预测的ORF(ORF 279.ng)有89.5%相同性(在152个氨基酸的重迭区):                 10       20      30      40       50       60m279.pep    ITRICGCLISTVFRASASLSAAGFIRLQWEGTDTGSGRARLAPASLAAAMARPTAAALPA          :||||||||||:  :|||||||||||||||||||||||||||||||||||:|||||||||g279       MTRICGCLISTVLSVSASLSAAGFIRLQWEGTDTGSGRARLAPASLAAAMVRPTAAALPA                 10       20      30      40       50       60                 70       80      90     100      110      120m279.pep    ITICPGELKLTASTTSLWAASAQMALTCSSSKPRIAAIAPTPCGTADCISSARRRTSLTA          || |||||||||||||  | |||: ||||||||::|||||||||||||||||||||||||g279       ITTCPGELKLTASTTSPCADSAQICLTCSSSKPKMAAIAPTPCGTADCISSARRRTSLTA                 70       80      90     100      110      120                130      140     150m279.pep    SAKFNAPAATSAVYSPRLCPATAAGVLPPASKX          ||| ||  ||||||||||||||||||||||:|||g279       SAKSNASAATSAVYSPRLCPATAAGVLPPTSKX                130      140     150下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 6>:a279.seq  1 ATGACNCNGA TTTGCGGCTG CTTGATTTCA ACGGTTTNNA GGGCTTCGGC 51 GAGTTTGTCG GCGGCGGGTT TCATGAGGCT GCAATGGGAA GGTACNGACA101 CNGGCAGCGG CAGGGCGCGT TTGGCGCCGG CTTCTTTGGC GGCAAGCATA151 GCGCGCTCGA CGGCGGCGGC ATTGCCTGCA ATCACGACTT GTCCGGGCGA201 GTTGAAGTTG ACGGCTTCAA CCACTTCATC CTGTGCGGAT TCGGCGCAAA251 TTTGTTTTAC CTGTTCATCT TCCAAGCCGA GAATCGCCGC CATTGCGCCC301 ACGCCTTGCG GTACGGCGGA CTGCATCAGT TCGGCGCGCA NGCGCACGAG351 TTTGACCGCG TCGGCAAAAT CCAATGCGCC GGCGGCAACN AGTGCGGTGT401 ATTCGCCGAN GCTGTGTCCG GCAACGGCGG CAGGCGTTTT GCCGCCCGCT451 TCCGAATAG这对应于氨基酸序列<SEQ ID 7;ORF 279.a>:a279.pep

  1 MTXICGCLIS TVXRASASLS AAGFMRLQWE GTDTGSGRAR LAPASLAASI 51 ARSTAAALPA ITTCPGELKL TASTTSSCAD SAQICFTCSS SKPRIAAIAP101 TPCGTADCIS SARXRTSLTA SAKSNAPAAT SAVYSPXLCP ATAAGVLPPA151 SE*m279/a279 ORFs 279和279.a有88.2%相同性(在152个氨基酸的重迭区)                 10       20      30       40      50       60m279.pep    ITRICGCLISTVFRASASLSAAGFIRLQWEGTDTGSGRARLAPASLAAAMARPTAAALPA          :|| ||||||||  |||||||||||:|||||||||||||||||||||||::|| |||||||a279       MTXICGCLISTVXRASASLSAAGFMRLQWEGTDTGSGRARLAPASLAASIARSTAAALPA                 10       20      30       40      50       60                 70       80      90      100     110      120m279.pep    ITICPGELKLTASTTSLWAASAQMALTCSSSKPRIAAIAPTPCGTADCISSARRRTSLTA          || |||||||||||||  | |||: :||||||||||||||||||||||||||| ||||||a279       ITTCPGELKLTASTTSSCADSAQICFTCSSSKPRIAAIAPTPCGTADCISSARXRTSLTA                 70       80      90      100     110      120                130      140     150m279.pep    SAKFNAPAATSAVYSPRLCPATAAGVLPPASKX          ||| |||||||||||| |||||||||||||||:|a279       SAKSNAPAATSAVYSPXLCPATAAGVLPPASEX                130      140     150519和519-1  gnm7.seq下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 8>:m519.seq(部分)  1..TCCGTTATCG GGCGTATGGA GTTGGACAAA ACGTTTGAAG AACGCGACGA 51  AATCAACAGT ACTGTTGTTG CGGCTTTGGA CGAGGCGGCC GGGgCTTgGG101  GTGTGAAGGT TTTGCGTTAT GAGATTAAAG ACTTGGTTCC GCCGCAAGAA151  ATCCTTCGCT CAATGCAGGC GCAAATTACT GCCGAACGCG AAAAACGCGC201  CCGTATCGCC GAATCCGAAG GTCGTAAAAT CGAACAAATC AACCTTGCCA251  GTGGTCAGCG CGAAGCCGAA ATCCAACAAT CCGAAGGCGA GGCTCAGGCT301  GCGGTCAATG CGTCAAATGC CGAGAAAATC GCCCGCATCA ACCGCGCCAA351  AGGTGAAGCG GAATCCTTGC GCCTTGTTGC CGAAGCCAAT GCCGAAGCCA401  TCCGTCAAAT TGCCGCCGCC CTTCAAACCC AAGGCGGTGC GGATGCGGTC451  AATCTGAAGA TTGCGGAACA ATACGTCGCT GCGTTCAACA ATCTTGCCAA501  AGAAAGCAAT ACGCTGATTA TGCCCGCCAA TGTTGCCGAC ATCGGCAGCC551  TGATTTCTGC CGGTATGAAA ATTATCGACA GCAGCAAAAC CGCCAAaTAA这对应于氨基酸序列<SEQ ID 9;ORF 519>:m519.pep(部分)  1..SVIGRMELDK TFEERDEINS TVVAALDEAA GAWGVKVLRY EIKDLVPPQE 51  ILRSMQAQIT AEREKRARIA ESEGRKIEQI NLASGQREAE IQQSEGEAQA101  AVNASNAEKI ARINRAKGEA ESLRLVAEAN AEAIRQIAAA LQTQGGADAV151  NLKIAEQYVA AFNNLAKESN TLIMPANVAD IGSLISAGMK IIDSSKTAK*下列部分DNA序列是在淋病奈瑟球菌中鉴别出的<SEQ ID 10>:g519.seq  1 atggaatttt tcattatctt gttggcagcc gtcgccgttt tcggcttcaa 51 atcctttgtc gtcatccccc agcaggaagt ccacgttgtc gaaaggctcg101 ggcgtttcca tcgcgccctg acggccggtt tgaatatttt gattcccttt151 atcgaccgcg tcgcctaccg ccattcgctg aaagaaatcc ctttagacgt201 acccagccag gtctgcatca cgcgcgataa tacgcaattg actgttgacg251 gcatcatcta tttccaagta accgatccca aactcgcctc atacggttcg301 agcaactaca ttatggcaat tacccagctt gcccaaacga cgctgcgttc351 cgttatcggg cgtatggagt tggacaaaac gtttgaagaa cgcgacgaaa401 tcaacagtac cgtcgtctcc gccctcgatg aagccgccgg ggcttggggt451 gtgaaagtcc tccgttacga aatcaaggat ttggttccgc cgcaagaaat501 ccttcgcgca atgcaggcac aaattaccgc cgaacgcgaa aaacgcgccc551 gtattgccga atccgaaggc cgtaaaatcg aacaaatcaa ccttgccagt601 ggtcagcgtg aagccgaaat ccaacaatcc gaaggcgagg ctcaggctgc651 ggtcaatgcg tccaatgccg agaaaatcgc ccgcatcaac cgcgccaaag701 gcgaagcgga atccctgcgc cttgttgccg aagccaatgc cgaagccaac

751 cgtcaaattg ccgccgccct tcaaacccaa agcggggcgg atgcggtcaa801 tctgaagatt gcgggacaat acgttaccgc gttcaaaaat cttgccaaag851 aagacaatac gcggattaag cccgccaagg ttgccgaaat cgggaaccct901 aattttcggc ggcatgaaaa attttcgcca gaagcaaaaa cggccaaata951 a这对应于氨基酸序列<SEQ ID 11;ORF 519.ng>:g519.pep  1 MEFFIILLAA VAVFGFKSFV VIPQQEVHVV ERLGRFHRAL TAGLNILIPF 51 IDRVAYRHSL KEIPLDVPSQ VCITRDNTQL TVDGIIYFQV TDPKLASYGS101 SNYIMAITQL AQTTLRSVIG RMELDKTFEE RDEINSTVVS ALDEAAGAWG151 VKVLRYEIKD LVPPQEILRA MQAQITAERE KRARIAESEG RKIEQINLAS201 GQREAEIQQS EGEAQAAVNA SNAEKIARIN RAKGEAESLR LVAEANAEAN251 RQIAAALQTQ SGADAVNLKI AGQYVTAFKN LAKEDNTRIK PAKVAEIGNP301 NFRRHEKFSP EAKTAK*ORF 519与淋病奈瑟球菌中预测的ORF(ORF 519.ng)有87.5%相同性(在200个氨基酸的重迭区):m519/g519                                          10       20      30m519.pep                             SVIGRMELDKTFEERDEINSTVVAALDEAA                                    |||||||||||||||||||||||:|||||g519        YFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAA             90      100     110      120      130     140                 40       50      60       70      80       90m519.pep    GAWGVKVLRYEIKDLVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAE          |||||||||||||||||||||||:|||||||||||||||||||||||||||||||||||||g519       GAWGVKVLRYEIKDLVPPQEILRAMQAQITAEREKRARIAESEGRKIEQINLASGQREAE           150      160      170     180      190      200                100      110      120      130      140     150m519.pep    IQQSEGEAQAAVNASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAV          |||||||||||||||||||||||||||||||||||||||||||  ||||||||||:|||||g519       IQQSEGEAQAAVNASNAEKIARINRAKGEAESLRLVAEANAEANRQIAAALQTQSGADAV           210      220      230     240      250      260                160      170      180      190      200m519.pep    NLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL-ISAGMKIIDSSKTAK          |||||  |||:||:|||||:|| | ||:||:||: :   |:   :||||g519       NLKIAGQYVTAFKNLAKEDNTRIKPAKVAEIGNPNFRRHEKFSPEAKTAK           270      280      290     300      310下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 12>:a519.seq  1 ATGGAATTTT TCATTATCTT GCTGGCAGCC GTCGTTGTTT TCGGCTTCAA 51 ATCCTTTGTT GTCATCCCAC AGCAGGAAGT CCACGTTGTC GAAAGGCTCG101 GGCGTTTCCA TCGCGCCCTG ACGGCCGGTT TGAATATTTT GATTCCCTTT151 ATCGACCGCG TCGCCTACCG CCATTCGCTG AAAGAAATCC CTTTAGACGT201 ACCCAGCCAG GTCTGCATCA CGCGCGACAA TACGCAGCTG ACTGTTGACG251 GTATCATCTA TTTCCAAGTA ACCGACCCCA AACTCGCCTC ATACGGTTCG301 AGCAACTACA TTATGGCGAT TACCCAGCTT GCCCAAACGA CGCTGCGTTC351 CGTTATCGGG CGTATGGAAT TGGACAAAAC GTTTGAAGAA CGCGACGAAA401 TCAACAGCAC CGTCGTCTCC GCCCTCGATG AAGCCGCCGG AGCTTGGGGT451 GTGAAGGTTT TGCGTTATGA GATTAAAGAC TTGGTTCCGC CGCAAGAAAT501 CCTTCGCTCA ATGCAGGCGC AAATTACTGC TGAACGCGAA AAACGCGCCC551 GTATCGCCGA ATCCGAAGGT CGTAAAATCG AACAAATCAA CCTTGCCAGT601 GGTCAGCGCG AAGCCGAAAT CCAACAATCC GAAGGCGAGG CTCAGGCTGC651 GGTCAATGCG TCAAATGCCG AGAAAATCGC CCGCATCAAC CGCGCCAAAG701 GTGAAGCGGA ATCCTTGCGC CTTGTTGCCG AAGCCAATGC CGAAGCCATC751 CGTCAAATTG CCGCCGCCCT TCAAACCCAA GGCGGTGCGG ATGCGGTCAA801 TCTGAAGATT GCGGAACAAT ACGTCGCCGC GTTCAACAAT CTTGCCAAAG851 AAAGCAATAC GCTGATTATG CCCGCCAATG TTGCCGACAT CGGCAGCCTG901 ATTTCTGCCG GTATGAAAAT TATCGACAGC AGCAAAACCG CCAAATAA

这对应于氨基酸序列<SEQ ID 13;ORF 519.a>:a519.pep  1 MEFFIILLAA VVVFGFKSFV VIPQQEVHVV ERLGRFHRAL TAGLNILIPF 51 IDRVAYRHSL KEIPLDVPSQ VCITRDNTQL TVDGIIYFQV TDPKLASYGS101 SNYIMAITQL AQTTLRSVIG RMELDKTFEE RDEINSTVVS ALDEAAGAWG151 VKVLRYEIKD LVPPQEILRS MQAQITAERE KRARIAESEG RKIEQINLAS201 GQREAEIQQS EGEAQAAVNA SNAEKIARIN RAKGEAESLR LVAEANAEAI251 RQIAAALQTQ GGADAVNLKI AEQYVAAFNN LAKESNTLIM PANVADIGSL301 ISAGMKIIDS SKTAK*m519/a519 ORFs 519和519.a有99.5%相同性(在199个氨基酸的重迭区)                                          10       20      30m519.pep                             SVIGRMELDKTFEERDEINSTVVAALDEAA                                   |||||||||||||||||||||||:||||||a519        YFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAA             90      100     110      120      130     140                 40       50      60       70      80       90m519.pep    GAWGVKVLRYEIKDLVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAE          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||a519       GAWGVKVLRYEIKDLVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAE           150      160     170      180      190      200                100      110      120      130     140      150m519.pep    IQQSEGEAQAAVNASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAV          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||a519       IQQSEGEAQAAVNASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAV           210      220      230     240      250      260                160      170      180     190      200m519.pep    NLKIAEQYVAAFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAKX          |||||||||||||||||||||||||||||||||||||||||||||||||||a519       NLKIAEQYVAAFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAKX           270      280      290     300      310进一步工作揭示,下列DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 14>:m519-1.seq  1 ATGGAATTTT TCATTATCTT GTTGGTAGCC GTCGCCGTTT TCGGTTTCAA 51 ATCCTTTGTT GTCATCCCAC AACAGGAAGT CCACGTTGTC GAAAGGCTGG101 GGCGTTTCCA TCGCGCCCTG ACGGcCGGTT TGAATATTTT GATTCCCTTT151 ATCGACCGCG TCGCCTACCG CCATTCGCTG AAAGAAATCC CTTTAGACGT201 ACCCAGCCAG GTCTGCATCA CGCGCGACAA TACGCAGCTG ACTGTTGACG251 GCATCATCTA TTTCCAAGTA ACCGACCCCA AACTCGCCTC ATACGGTTCG301 AGCAACTACA TTATGGCGAT TACCCAGCTT GCCCAAACGA CGCTGCGTTC351 CGTTATCGGG CGTATGGAGT TGGACAAAAC GTTTGAAGAA CGCGACGAAA401 TCAACAGTAC TGTTGTTGCG GCTTTGGACG AGGCGGCCGG GGCTTGGGGT451 GTGAAGGTTT TGCGTTATGA GATTAAAGAC TTGGTTCCGC CGCAAGAAAT501 CCTTCGCTCA ATGCAGGCGC AAATTACTGC CGAACGCGAA AAACGCGCCC551 GTATCGCCGA ATCCGAAGGT CGTAAAATCG AACAAATCAA CCTTGCCAGT601 GGTCAGCGCG AAGCCGAAAT CCAACAATCC GAAGGCGAGG CTCAGGCTGC651 GGTCAATGCG TCAAATGCCG AGAAAATCGC CCGCATCAAC CGCGCCAAAG701 GTGAAGCGGA ATCCTTGCGC CTTGTTGCCG AAGCCAATGC CGAAGCCATC751 CGTCAAATTG CCGCCGCCCT TCAAACCCAA GGCGGTGCGG ATGCGGTCAA801 TCTGAAGATT GCGGAACAAT ACGTCGCTGC GTTCAACAAT CTTGCCAAAG851 AAAGCAATAC GCTGATTATG CCCGCCAATG TTGCCGACAT CGGCAGCCTG901 ATTTCTGCCG GTATGAAAAT TATCGACAGC AGCAAAACCG CCAAATAA这对应于氨基酸序列<SEQ ID 15;ORF 519-1>:m519-1.

  1 MEFFIILLVA VAVFGFKSFV VIPQQEVHVV ERLGRFHRAL TAGLNILIPF 51 IDRVAYRHSL KEIPLDVPSQ VCITRDNTQL TVDGIIYFQV TDPKLASYGS101 SNYIMAITQL AQTTLRSVIG RMELDKTFEE RDEINSTVVA ALDEAAGAWG151 VKVLRYEIKD LVPPQEILRS MQAQITAERE KRARIAESEG RKIEQINLAS201 GQREAEIQQS EGEAQAAVNA SNAEKIARIN RAKGEAESLR LVAEANAEAI251 RQIAAALQTQ GGADAVNLKI AEQYVAAFNN LAKESNTLIM PANVADIGSL301 ISAGMKIIDS SKTAK*

下列DNA序列是在淋病奈瑟球菌中鉴别出的<SEQ ID 16>:g519-1.seq  1 ATGGAATTTT TCATTATCTT GTTGGCAGCC GTCGCCGTTT TCGGCTTCAA 51 ATCCTTTGTC GTCATCCCCC AGCAGGAAGT CCACGTTGTC GAAAGGCTCG101 GGCGTTTCCA TCGCGCCCTG ACGGCCGGTT TGAATATTTT GATTCCCTTT151 ATCGACCGCG TCGCCTACCG CCATTCGCTG AAAGAAATCC CTTTAGACGT201 ACCCAGCCAG GTCTGCATCA CGCGCGATAA TACGCAATTG ACTGTTGACG251 GCATCATCTA TTTCCAAGTA ACCGATCCCA AACTCGCCTC ATACGGTTCG301 AGCAACTACA TTATGGCAAT TACCCAGCTT GCCCAAACGA CGCTGCGTTC351 CGTTATCGGG CGTATGGAGT TGGACAAAAC GTTTGAAGAA CGCGACGAAA401 TCAACAGTAC CGTCGTCTCC GCCCTCGATG AAGCCGCCGG GGCTTGGGGT451 GTGAAAGTCC TCCGTTACGA AATCAAGGAT TTGGTTCCGC CGCAAGAAAT501 CCTTCGCGCA ATGCAGGCAC AAATTACCGC CGAACGCGAA AAACGCGCCC551 GTATTGCCGA ATCCGAAGGC CGTAAAATCG AACAAATCAA CCTTGCCAGT601 GGTCAGCGTG AAGCCGAAAT CCAACAATCC GAAGGCGAGG CTCAGGCTGC651 GGTCAATGCG TCCAATGCCG AGAAAATCGC CCGCATCAAC CGCGCCAAAG701 GCGAAGCGGA ATCCCTGCGC CTTGTTGCCG AAGCCAATGC CGAAGCCATC751 CGTCAAATTG CCGCCGCCCT TCAAACCCAA GGCGGGGCGG ATGCGGTCAA801 TCTGAAGATT GCGGAACAAT ACGTAGCCGC GTTCAACAAT CTTGCCAAAG851 AAAGCAATAC GCTGATTATG CCCGCCAATG TTGCCGACAT CGGCAGCCTG901 ATTTCTGCCG GCATGAAAAT TATCGACAGC AGCAAAACCG CCAAATAA这对应于氨基酸序列<SEQ ID 17;ORF 519-1.ng>:g519-1.pep  1 MEFFIILLAA VAVFGFKSFV VIPQQEVHVV ERLGRFHRAL TAGLNILIPF 51 IDRVAYRHSL KEIPLDVPSQ VCITRDNTQL TVDGIIYFQV TDPKLASYGS101 SNYIMAITQL AQTTLRSVIG RMELDKTFEE RDEINSTVVS ALDEAAGAWG151 VKVLRYEIKD LVPPQEILRA MQAQITAERE KRARIAESEG RKIEQINLAS201 GQREAEIQQS EGEAQAAVNA SNAEKIARIN RAKGEAESLR LVAEANAEAI251 RQIAAALQTQ GGADAVNLKI AEQYVAAFNN LAKESNTLIM PANVADIGSL301 ISAGMKIIDS SKTAK*m519-1/g519-1 ORFs 519-1和519-1.ng有99.0%相同性(在315个氨基酸的重迭区)                   10      20       30       40      50       60g519-1.pep    MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGLNILIPFIDRVAYRHSL            ||||||||:|||||||||||||||||||||||||||||||||||||||||||||||||||m519-1       MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGLNILIPFIDRVAYRHSL                   10      20       30       40      50       60                   70      80       90      100     110      120g519-1.pep    KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m519-1       KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG                   70      80       90      100     110      120                  130     140      150      160     170      180g519-1.pep    RMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKDLVPPQEILRAMQAQITAERE            |||||||||||||||||||:|||||||||||||||||||||||||||||:||||||||||m519-1       RMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE                  130     140      150      160     170      180                  190     200      210      220     230      240 

g519-1.pep    KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m519-1       KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR                  190     200      210      220     230      240 

                  250     260      270      280     290      300 

g519-1.pep    LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m519-1       LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL                  250     260      270      280     290      300                  310g519-1.pep    ISAGMKIIDSSKTAKX            ||||||||||||||||

m519-1  ISAGMKIIDSSKTAKX             310下列DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 18>:a519-1.seq  1 ATGGAATTTT TCATTATCTT GCTGGCAGCC GTCGTTGTTT TCGGCTTCAA 51 ATCCTTTGTT GTCATCCCAC AGCAGGAAGT CCACGTTGTC GAAAGGCTCG101 GGCGTTTCCA TCGCGCCCTG ACGGCCGGTT TGAATATTTT GATTCCCTTT151 ATCGACCGCG TCGCCTACCG CCATTCGCTG AAAGAAATCC CTTTAGACGT201 ACCCAGCCAG GTCTGCATCA CGCGCGACAA TACGCAGCTG ACTGTTGACG251 GTATCATCTA TTTCCAAGTA ACCGACCCCA AACTCGCCTC ATACGGTTCG301 AGCAACTACA TTATGGCGAT TACCCAGCTT GCCCAAACGA CGCTGCGTTC351 CGTTATCGGG CGTATGGAAT TGGACAAAAC GTTTGAAGAA CGCGACGAAA401 TCAACAGCAC CGTCGTCTCC GCCCTCGATG AAGCCGCCGG AGCTTGGGGT451 GTGAAGGTTT TGCGTTATGA GATTAAAGAC TTGGTTCCGC CGCAAGAAAT501 CCTTCGCTCA ATGCAGGCGC AAATTACTGC TGAACGCGAA AAACGCGCCC551 GTATCGCCGA ATCCGAAGGT CGTAAAATCG AACAAATCAA CCTTGCCAGT601 GGTCAGCGCG AAGCCGAAAT CCAACAATCC GAAGGCGAGG CTCAGGCTGC651 GGTCAATGCG TCAAATGCCG AGAAAATCGC CCGCATCAAC CGCGCCAAAG701 GTGAAGCGGA ATCCTTGCGC CTTGTTGCCG AAGCCAATGC CGAAGCCATC751 CGTCAAATTG CCGCCGCCCT TCAAACCCAA GGCGGTGCGG ATGCGGTCAA801 TCTGAAGATT GCGGAACAAT ACGTCGCCGC GTTCAACAAT CTTGCCAAAG851 AAAGCAATAC GCTGATTATG CCCGCCAATG TTGCCGACAT CGGCAGCCTG901 ATTTCTGCCG GTATGAAAAT TATCGACAGC AGCAAAACCG CCAAATAA这对应于氨基酸序列<SEQ ID 19;ORF 519-1.a>:a519-1.pep.

  1 MEFFIILLAA VVVFGFKSFV VIPQQEVHVV ERLGRFHRAL TAGLNILIPF 51 IDRVAYRHSL KEIPLDVPSQ VCITRDNTQL TVDGIIYFQV TDPKLASYGS101 SNYIMAITQL AQTTLRSVIG RMELDKTFEE RDEINSTVVS ALDEAAGAWG151 VKVLRYEIKD LVPPQEILRS MQAQITAERE KRARIAESEG RKIEQINLAS201 GQREAEIQQS EGEAQAAVNA SNAEKIARIN RAKGEAESLR LVAEANAEAI251 RQIAAALQTQ GGADAVNLKI AEQYVAAFNN LAKESNTLIM PANVADIGSL301 ISAGMKIIDS SKTAK*m519-1/a519-1 ORFs 519-1和519-1.a有99.0%相同性(在315个氨基酸的重迭区)                   10       20      30       40      50       60a519-1.pep    MEFFIILLAAVVVFGFKSFVVIPQQEVHVVERLGRFHRALTAGLNILIPFIDRVAYRHSL            ||||||||:||:||||||||||||||||||||||||||||||||||||||||||||||||m519-1       MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGLNILIPFIDRVAYRHSL                   10       20      30       40      50       60                   70       80      90      100     110      120a519-1.pep    KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m519-1       KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG                   70       80      90      100     110      120                  130      140     150      160     170      180a519-1.pep    RMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE            |||||||||||||||||||:||||||||||||||||||||||||||||||||||||||||m519-1       RMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE                  130      140     150      160     170      180                  190      200     210      220     230      240a519-1.pep    KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m519-1       KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR                  190      200     210      220     230      240                  250      260     270      280     290      300a519-1.pep    LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m519-1       LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL                  250      260     270      280     290      300

                  310a519-1.pep    ISAGMKIIDSSKTAKX            ||||||||||||||||m519-1       ISAGMKIIDSSKTAKX                  310576和576-1g    nm22.seq下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 20>:m576.seq..(部分)  1..ATGCAGCAGG CAAGCTATGC GATGGGCGTG GACATCGGAC GCTCCCTGAA 51  GCAAATGAAG GAACAGGGCG CGGAAATCGA TTTGAAAGTC TTTACCGAAG101  CCATGCAGGC AGTGTATGAC GGCAAAGAAA TCAAAATGAC CGAAGAGCAG151  GCTCAGGAAG TCATGATGAA ATTCCTTCAG GAACAACAGG CTAAAGCCGT201  AGAAAAACAC AAGGCGGACG CGAAGGCCAA TAAAGAAAAA GGCGAAGCCT251  TTCTGAAAGA AAATGCCGCC AAAGACGGCG TGAAGACCAC TGCTTCCGGC301  CTGCAATACA AAATCACCAA ACAGGGCGAA GGCAAACAGC CGACCAAAGA351  CGACATCGTT ACCGTGGAAT ACGAAGGCCG CCTGATTGAC GGTACGGTAT401  TCGACAGCAG CAAAGCCAAC GGCGGCCCGG TCACCTTCCC TTTGAGCCAA451  GTGATTCCGG GTTGGACCGA AGgCGTACAG CTTCTGAAAG AAGGCGGCGA501  AGCCACGTTC TACATCCCGT CCAACCTTGC CTACCGCGAA CAGGGTGCGG551  GCGACAAAAT CGGTCCGAAC GCCACTTTGG TATTTGATGT GAAACTGGTC601  AAAATCGGCG CACCCGAAAA CGCGCCCGCC AAGCAGCCGG CTCAAGTCGA651  CATCAAAAAA GTAAATTAA这对应于氨基酸序列<SEQ ID 21;ORF 576>:m576.pep..(部分)  1..MQQASYAMGV DIGRSLKQMK EQGAEIDLKV FTEAMQAVYD GKEIKMTEEQ 51  AQEVMMKFLQ EQQAKAVEKH KADAKANKEK GEAFLKENAA KDGVKTTASG101  LQYKITKQGE GKQPTKDDIV TVEYEGRLID GTVFDSSKAN GGPVTFPLSQ151  VIPGWTEGVQ LLKEGGEATF YIPSNLAYRE QGAGDKIGPN ATLVFDVKLV201  KIGAPENAPA KQPAQVDIKK VN*下列部分DNA序列是在淋病奈瑟球菌中鉴别出的<SEQ ID 22>:g576.seq..(部分)  1..atgggcgtgg acatcggacg ctccctgaaa caaatgaagg aacagggcgc 51  ggaaatcgat ttgaaagtct ttaccgatgc catgcaggca gtgtatgacg101  gcaaagaaat caaaatgacc gaagagcagg cccaggaagt gatgatgaaa151  ttcctgcagg agcagcaggc taaagccgta gaaaaacaca aggcggatgc201  gaaggccaac aaagaaaaag gcgaagcctt cctgaaggaa aatgccgccg251  aagacggcgt gaagaccact gcttccggtc tgcagtacaa aatcaccaaa301  cagggtgaag gcaaacagcc gacaaaagac gacatcgtta ccgtggaata351  cgaaggccgc ctgattgacg gtaccgtatt cgacagcagc aaagccaacg401  gcggcccggc caccttccct ttgagccaag tgattccggg ttggaccgaa451  ggcgtacggc ttctgaaaga aggcggcgaa gccacgttct acatcccgtc501  caaccttgcc taccgcgaac agggtgcggg cgaaaaaatc ggtccgaacg551  ccactttggt atttgacgtg aaactggtca aaatcggcgc acccgaaaac601  gcgcccgcca agcagccgga tcaagtcgac atcaaaaaag taaattaa这对应于氨基酸序列<SEQ ID 23;ORF 576.ng>:g576.pep..(部分)  1..MGVDIGRSLK QMKEQGAEID LKVFTDAMQA VYDGKEIKMT EEQAQEVMMK 51  FLQEQQAKAV EKHKADAKAN KEKGEAFLKE NAAEDGVKTT ASGLQYKITK101  QGEGKQPTKD DIVTVEYEGR LIDGTVFDSS KANGGPATFP LSQVIPGWTE151  GVRLLKEGGE ATFYIPSNLA YREQGAGEKI GPNATLVFDV KLVKIGAPEN201  APAKQPDQVD IKKVN*对该氨基酸序列的计算机分析给出下列结果:

与淋病奈瑟球菌中预测ORF的同源性m576/g576  97.2%相同性(在215个氨基酸的重迭区)                 10       20      30       40       50      60m576.pep    MQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMMKFLQ                |||||||||||||||||||||||||:|||||||||||||||||||||||||||g576             MGVDIGRSLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQEVMMKFLQ                       10      20       30      40       50                 70       80      90      100      110     120m576.pep    EQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQPTKDDIV          ||||||||||||||||||||||||||||||:|||||||||||||||||||||||||||||g576       EQQAKAVEKHKADAKANKEKGEAFLKENAAEDGVKTTASGLQYKITKQGEGKQPTKDDIV              60       70      80       90      100     110                130      140      150     160      170      180m576.pep    TVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYIPSNLAYRE          |||||||||||||||||||||||:||||||||||||||||:|||||||||||||||||||g576       TVEYEGRLIDGTVFDSSKANGGPATFPLSQVIPGWTEGVRLLKEGGEATFYIPSNLAYRE             120      130      140      150      160     170                190      200      210      220m576.pep    QGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVNX          ||||:|||||||||||||||||||||||||||| |||||||||g576       QGAGEKIGPNATLVFDVKLVKIGAPENAPAKQPDQVDIKKVNX             180      190      200      210下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 24>:a576.seq  1 ATGAACACCA TTTTCAAAAT CAGCGCACTG ACCCTTTCCG CCGCTTTGGC 51 ACTTTCCGCC TGCGGCAAAA AAGAAGCCGC CCCCGCATCT GCATCCGAAC101 CTGCCGCCGC TTCTTCCGCG CAGGGCGACA CCTCTTCGAT CGGCAGCACG151 ATGCAGCAGG CAAGCTATGC GATGGGCGTG GACATCGGAC GCTCCCTGAA201 GCAAATGAAG GAACAGGGCG CGGAAATCGA TTTGAAAGTC TTTACCGAAG251 CCATGCAGGC AGTGTATGAC GGCAAAGAAA TCAAAATGAC CGAAGAGCAG301 GCTCAGGAAG TCATGATGAA ATTCCTTCAG GAACAACAGG CTAAAGCCGT351 AGAAAAACAC AAGGCGGACG CGAAGGCCAA TAAAGAAAAA GGCGAAGCCT401 TTCTGAAAGA AAATGCCGCC AAAGACGGCG TGAAGACCAC TGCTTCCGGC451 CTGCAATACA AAATCACCAA ACAGGGCGAA GGCAAACAGC CGACCAAAGA501 CGACATCGTT ACCGTGGAAT ACGAAGGCCG CCTGATTGAC GGTACGGTAT551 TCGACAGCAG CAAAGCCAAC GGCGGCCCGG TCACCTTCCC TTTGAGCCAA601 GTGATTCTGG GTTGGACCGA AGGCGTACAG CTTCTGAAAG AAGGCGGCGA651 AGCCACGTTC TACATCCCGT CCAACCTTGC CTACCGCGAA CAGGGTGCGG701 GCGACAAAAT CGGCCCGAAC GCCACTTTGG TATTTGATGT GAAACTGGTC751 AAAATCGGCG CACCCGAAAA CGCGCCCGCC AAGCAGCCGG CTCAAGTCGA801 CATCAAAAAA GTAAATTAA这对应于氨基酸序列<SEQ ID 25;ORF 576.a>:a576.pep  1 MNTIFKISAL TLSAALALSA CGKKEAAPAS ASEPAAASSA QGDTSSIGST 51 MQQASYAMGV DIGRSLKQMK EQGAEIDLKV FTEAMQAVYD GKEIKMTEEQ101 AQEVMMKFLQ EQQAKAVEKH KADAKANKEK GEAFLKENAA KDGVKTTASG151 LQYKITKQGE GKQPTKDDIV TVEYEGRLID GTVFDSSKAN GGPVTFPLSQ201 VILGWTEGVQ LLKEGGEATF YIPSNLAYRE QGAGDKIGPN ATLVFDVKLV251 KIGAPENAPA KQPAQVDIKK VN*m576/a576  ORFs 576和576.a有99.5%相同性(在222个氨基酸的重迭区)                                         10       20      30m576.pep                            MQQASYAMGVDIGRSLKQMKEQGAEIDLKV                                  ||||||||||||||||||||||||||||||a576       CGKKEAAPASASEPAAASSAQGDTSSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKV                 30       40      50       60      70       80                 40       50      60       70      80       90m576.pep    FTEAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAA          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||a576       FTEAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAA                 90     100      110      120      130      140

                100      110      120     130      140      150m576.pep    KDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQ          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||a576       KDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQ                150      160      170     180      190      200                160      170      180     190      200      210m576.pep    VIPGWTEGVQLLKEGGEATFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPA          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||a576       VILGWTEGVQLLKEGGEATFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPA                210      220      230     240      250      260                220m576.pep    KQPAQVDIKKVNX          |||||||||||||a576       KQPAQVDIKKVNX                270进一步工作揭示,下列DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 26>:    m576-1.seq  1 ATGAACACCA TTTTCAAAAT CAGCGCACTG ACCCTTTCCG CCGCTTTGGC 51 ACTTTCCGCC TGCGGCAAAA AAGAAGCCGC CCCCGCATCT GCATCCGAAC101 CTGCCGCCGC TTCTTCCGCG CAGGGCGACA CCTCTTCGAT CGGCAGCACG151 ATGCAGCAGG CAAGCTATGC GATGGGCGTG GACATCGGAC GCTCCCTGAA201 GCAAATGAAG GAACAGGGCG CGGAAATCGA TTTGAAAGTC TTTACCGAAG251 CCATGCAGGC AGTGTATGAC GGCAAAGAAA TCAAAATGAC CGAAGAGCAG301 GCTCAGGAAG TCATGATGAA ATTCCTTCAG GAACAACAGG CTAAAGCCGT351 AGAAAAACAC AAGGCGGACG CGAAGGCCAA TAAAGAAAAA GGCGAAGCCT401 TTCTGAAAGA AAATGCCGCC AAAGACGGCG TGAAGACCAC TGCTTCCGGC451 CTGCAATACA AAATCACCAA ACAGGGCGAA GGCAAACAGC CGACCAAAGA501 CGACATCGTT ACCGTGGAAT ACGAAGGCCG CCTGATTGAC GGTACGGTAT551 TCGACAGCAG CAAAGCCAAC GGCGGCCCGG TCACCTTCCC TTTGAGCCAA601 GTGATTCCGG GTTGGACCGA AGGCGTACAG CTTCTGAAAG AAGGCGGCGA651 AGCCACGTTC TACATCCCGT CCAACCTTGC CTACCGCGAA CAGGGTGCGG701 GCGACAAAAT CGGTCCGAAC GCCACTTTGG TATTTGATGT GAAACTGGTC751 AAAATCGGCG CACCCGAAAA CGCGCCCGCC AAGCAGCCGG CTCAAGTCGA801 CATCAAAAAA GTAAATTAA这对应于氨基酸序列<SEQ ID 27;ORF 576-1>:m576-1.pep  1 MNTIFKISAL TLSAALALSA CGKKEAAPAS ASEPAAASSA QGDTSSIGST 51 MQQASYAMGV DIGRSLKQMK EQGAEIDLKV FTEAMQAVYD GKEIKMTEEQ101 AQEVMMKFLQ EQQAKAVEKH KADAKANKEK GEAFLKENAA KDGVKTTASG151 LQYKITKQGE GKQPTKDDIV TVEYEGRLID GTVFDSSKAN GGPVTFPLSQ201 VIPGWTEGVQ LLKEGGEATF YIPSNLAYRE QGAGDKIGPN ATLVFDVKLV251 KIGAPENAPA KQPAQVDIKK VN*下列DNA序列是在淋病奈瑟球菌中鉴别出的<SEQ ID 28>:g576-1.seq  1 ATGAACACCA TTTTCAAAAT CAGCGCACTG ACCCTTTCCG CCGCTTTGGC 51 ACTTTCCGCC TGCGGCAAAA AAGAAGCCGC CCCCGCATCT GCATCCGAAC101 CTGCCGCCGC TTCTGCCGCG CAGGGCGACA CCTCTTCAAT CGGCAGCACG151 ATGCAGCAGG CAAGCTATGC AATGGGCGTG GACATCGGAC GCTCCCTGAA201 ACAAATGAAG GAACAGGGCG CGGAAATCGA TTTGAAAGTC TTTACCGATG251 CCATGCAGGC AGTGTATGAC GGCAAAGAAA TCAAAATGAC CGAAGAGCAG301 GCCCAGGAAG TGATGATGAA ATTCCTGCAG GAGCAGCAGG CTAAAGCCGT351 AGAAAAACAC AAGGCGGATG CGAAGGCCAA CAAAGAAAAA GGCGAAGCCT401 TCCTGAAGGA AAATGCCGCC AAAGACGGCG TGAAGACCAC TGCTTCCGGT451 CTGCAGTACA AAATCACCAA ACAGGGTGAA GGCAAACAGC CGACAAAAGA501 CGACATCGTT ACCGTGGAAT ACGAAGGCCG CCTGATTGAC GGTACCGTAT551 TCGACAGCAG CAAAGCCAAC GGCGGCCCGG CCACCTTCCC TTTGAGCCAA601 GTGATTCCGG GTTGGACCGA AGGCGTACGG CTTCTGAAAG AAGGCGGCGA651 AGCCACGTTC TACATCCCGT CCAACCTTGC CTACCGCGAA CAGGGTGCGG701 GCGAAAAAAT CGGTCCGAAC GCCACTTTGG TATTTGACGT GAAACTGGTC751 AAAATCGGCG CACCCGAAAA CGCGCCCGCC AAGCAGCCGG ATCAAGTCGA

801 CATCAAAAAA GTAAATTAA这对应于氨基酸序列<SEQ ID 29;ORF 576-1.ng>:g576-1.pep  1 MNTIFKISAL TLSAALALSA CGKKEAAPAS ASEPAAASAA QGDTSSIGST 51 MQQASYAMGV DIGRSLKQMK EQGAEIDLKV FTDAMQAVYD GKEIKMTEEQ101 AQEVMMKFLQ EQQAKAVEKH KADAKANKEK GEAFLKENAA KDGVKTTASG151 LQYKITKQGE GKQPTKDDIV TVEYEGRLID GTVFDSSKAN GGPATFPLSQ201 VIPGWTEGVR LLKEGGEATF YIPSNLAYRE QGAGEKIGPN ATLVFDVKLV251 KIGAPENAPA KQPDQVDIKK VN*g576-1/m576-1 ORFs 576-1和576-1.ng有97.8%相同性(在272个氨基酸的重迭区)                   10       20      30       40      50      60g576-1.pep    MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASAAQGDTSSIGSTMQQASYAMGV            ||||||||||||||||||||||||||||||||||||||:|||||||||||||||||||||m576-1       MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDTSSIGSTMQQASYAMGV                   10       20      30       40      50      60                   70       80      90      100     110     120g576-1.pep    DIGRSLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKH            ||||||||||||||||||||||:|||||||||||||||||||||||||||||||||||||m576-1       DIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKH                   70       80      90      100     110     120                  130      140     150      160     170     180g576-1.pep    KADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLID            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m576-1       KADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLID                  130      140      150     160     170     180                  190      200      210     220     230     240g576-1.pep    GTVFDSSKANGGPATFPLSQVIPGWTEGVRLLKEGGEATFYIPSNLAYREQGAGEKIGPN            |||||||||||||:|||||||||||||||:||||||||||||||||||||||||:|||||m576-1       GTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYIPSNLAYREQGAGDKIGPN                  190      200      210     220     230     240                  250      260      270g576-1.pep    ATLVFDVKLVKIGAPENAPAKQPDQVDIKKVNX            ||||||||||||||||||||||| |||||||||m576-1       ATLVFDVKLVKIGAPENAPAKQPAQVDIKKVNX                  250      260      270下列DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 30>:a576-1.seq  1 ATGAACACCA TTTTCAAAAT CAGCGCACTG ACCCTTTCCG CCGCTTTGGC 51 ACTTTCCGCC TGCGGCAAAA AAGAAGCCGC CCCCGCATCT GCATCCGAAC101 CTGCCGCCGC TTCTTCCGCG CAGGGCGACA CCTCTTCGAT CGGCAGCACG151 ATGCAGCAGG CAAGCTATGC GATGGGCGTG GACATCGGAC GCTCCCTGAA201 GCAAATGAAG GAACAGGGCG CGGAAATCGA TTTGAAAGTC TTTACCGAAG251 CCATGCAGGC AGTGTATGAC GGCAAAGAAA TCAAAATGAC CGAAGAGCAG301 GCTCAGGAAG TCATGATGAA ATTCCTTCAG GAACAACAGG CTAAAGCCGT351 AGAAAAACAC AAGGCGGACG CGAAGGCCAA TAAAGAAAAA GGCGAAGCCT401 TTCTGAAAGA AAATGCCGCC AAAGACGGCG TGAAGACCAC TGCTTCCGGC451 CTGCAATACA AAATCACCAA ACAGGGCGAA GGCAAACAGC CGACCAAAGA501 CGACATCGTT ACCGTGGAAT ACGAAGGCCG CCTGATTGAC GGTACGGTAT551 TCGACAGCAG CAAAGCCAAC GGCGGCCCGG TCACCTTCCC TTTGAGCCAA601 GTGATTCTGG GTTGGACCGA AGGCGTACAG CTTCTGAAAG AAGGCGGCGA651 AGCCACGTTC TACATCCCGT CCAACCTTGC CTACCGCGAA CAGGGTGCGG701 GCGACAAAAT CGGCCCGAAC GCCACTTTGG TATTTGATGT GAAACTGGTC751 AAAATCGGCG CACCCGAAAA CGCGCCCGCC AAGCAGCCGG CTCAAGTCGA801 CATCAAAAAA GTAAATTAA这对应于氨基酸序列<SEQ ID 31;ORF 576-1.a>:a576-1.pep  1 MNTIFKISAL TLSAALALSA CGKKEAAPAS ASEPAAASSA QGDTSSIGST 51 MQQASYAMGV DIGRSLKQMK EQGAEIDLKV FTEAMQAVYD GKEIKMTEEQ

101 AQEVMMKFLQ EQQAKAVEKH KADAKANKEK GEAFLKENAA KDGVKTTASG151 LQYKITKQGE GKQPTKDDIV TVEYEGRLID GTVFDSSKAN GGPVTFPLSQ201 VILGWTEGVQ LLKEGGEATF YIPSNLAYRE QGAGDKIGPN ATLVFDVKLV251 KIGAPENAPA KQPAQVDIKK VN*a576-1/m576-1 ORFs 576-1和576-1.a 99.6%相同性(在272个氨基酸的重迭区)                   10       20      30       40      50       60a576-1.pep    MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDTSSIGSTMQQASYAMGV            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m576-1       MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDTSSIGSTMQQASYAMGV                   10       20      30       40      50       60                   70       80      90      100     110      120a576-1.pep    DIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKH            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m576-1       DIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKH                   70       80      90      100     110      120                  130      140     150      160     170      180a576-1.pep    KADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLID            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m576-1       KADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLID                  130      140     150      160     170      180                  190      200     210      220     230      240a576-1.pep    GTVFDSSKANGGPVTFPLSQVILGWTEGVQLLKEGGEATFYIPSNLAYREQGAGDKIGPN            |||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||m576-1       GTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYIPSNLAYREQGAGDKIGPN                  190      200     210      220     230      240                  250      260     270a576-1.pep    ATLVFDVKLVKIGAPENAPAKQPAQVDIKKVNX            |||||||||||||||||||||||||||||||||m576-1       ATLVFDVKLVKIGAPENAPAKQPAQVDIKKVNX                  250      260     270919和919-2      gnm43.seq下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 32>:m919.seq   1 ATGAAAAAAT ACCTATTCCG CGCCGCCCTG TACGGCATCG CCGCCGCCAT  51 CCTCGCCGCC TGCCAAAGCA AGAGCATCCA AACCTTTCCG CAACCCGACA 101 CATCCGTCAT CAACGGCCCG GACCGGCCGG TCGGCATCCC CGACCCCGCC 151 GGAACGACGG TCGGCGGCGG CGGGGCCGTC TATACCGTTG TACCGCACCT 201 GTCCCTGCCC CACTGGGCGG CGCAGGATTT CGCCAAAAGC CTGCAATCCT 251 TCCGCCTCGG CTGCGCCAAT TTGAAAAACC GCCAAGGCTG GCAGGATGTG 301 TGCGCCCAAG CCTTTCAAAC CCCCGTCCAT TCCTTTCAGG CAAAACAGTT 351 TTTTGAACGC TATTTCACGC CGTGGCAGGT TGCAGGCAAC GGAAGCCTTG 401 CCGGTACGGT TACCGGCTAT TACGAACCGG TGCTGAAGGG CGACGACAGG 451 CGGACGGCAC AAGCCCGCTT CCCGATTTAC GGTATTCCCG ACGATTTTAT 501 CTCCGTCCCC CTGCCTGCCG GTTTGCGGAG CGGAAAAGCC CTTGTCCGCA 551 TCAGGCAGAC GGGAAAAAAC AGCGGCACAA TCGACAATAC CGGCGGCACA 601 CATACCGCCG ACCTCTCCcG ATTCCCCATC ACCGCGCGCA CAACAGCAAT 651 CAAAGGCAGG TTTGAAGGAA GCCGCTTCCT CCCCTACCAC ACGCGCAACC 701 AAATCAACGG CGGCGCGCTT GACGGCAAAG CCCCGATACT CGGTTACGCC 751 GAAGACCCTG TCGAACTTTT TTTTATGCAC ATCCAAGGCT CGGGCCGTCT 801 GAAAACCCCG TCCGGCAAAT ACATCCGCAT CGGCTATGCC GACAAAAACG 851 AACATCCyTA CGTTTCCATC GGACGCTATA TGGCGGATAA GGGCTACCTC 901 AAACTCGGAC AAACCTCCAT GCAGGGCATT AAGTCTTATA TGCGGCAAAA 951 TCCGCAACGC CTCGCCGAAG TTTTGGGTCA AAACCCCAGC TATATCTTTT1001 TCCGCGAGCT TGCCGGAAGC AGCAATGACG GCCCTGTCGG CGCACTGGGC1051 ACGCCGCTGA TGGGGGAATA TGCCGGCGCA GTCGACCGGC ACTACATTAC1101 CTTGGGTGCG CCCTTATTTG TCGCCACCGC CCATCCGGTT ACCCGCAAAG1151 CCCTCAACCG CCTGATTATG GCGCAGGATA CCGGCAGCGC GATTAAAGGC1201 GCGGTGCGCG TGGATTATTT TTGGGGATAC GGCGACGAAG CCGGCGAACT1251 TGCCGGCAAA CAGAAAACCA CGGGATATGT CTGGCAGCTC CTACCCAACG1301 GTATGAAGCC CGAATACCGc CCGTAA

这对应于氨基酸序列<SEQ ID 33;ORF 919>:m919.pep  1 MKKYLFRAAL YGIAAAILAA CQSKSIQTFP QPDTSVINGP DRPVGIPDPA 51 GTTVGGGGAV YTVVPHLSLP HWAAQDFAKS LQSFRLGCAN LKNRQGWQDV101 CAQAFQTPVH SFQAKQFFER YFTPWQVAGN GSLAGTVTGY YEPVLKGDDR151 RTAQARFPIY GIPDDFISVP LPAGLRSGKA LVRIRQTGKN SGTIDNTGGT201 HTADLSRFPI TARTTAIKGR FEGSRFLPYH TRNQINGGAL DGKAPILGYA251 EDPVELFFMH IQGSGRLKTP SGKYIRIGYA DKNEHPYVSI GRYMADKGYL301 KLGQTSMQGI KSYMRQNPQR LAEVLGQNPS YIFFRELAGS SNDGPVGALG351 TPLMGEYAGA VDRHYITLGA PLFVATAHPV TRKALNRLIM AQDTGSAIKG401 AVRVDYFWGY GDEAGELAGK QKTTGYVWQL LPNGMKPEYR P*下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 34>:m919-2.seq   1 ATGAAAAAAT ACCTATTCCG CGCCGCCCTG TACGGCATCG CCGCCGCCAT  51 CCTCGCCGCC TGCCAAAGCA AGAGCATCCA AACCTTTCCG CAACCCGACA 101 CATCCGTCAT CAACGGCCCG GACCGGCCGG TCGGCATCCC CGACCCCGCC 151 GGAACGACGG TCGGCGGCGG CGGGGCCGTC TATACCGTTG TACCGCACCT 201 GTCCCTGCCC CACTGGGCGG CGCAGGATTT CGCCAAAAGC CTGCAATCCT 251 TCCGCCTCGG CTGCGCCAAT TTGAAAAACC GCCAAGGCTG GCAGGATGTG 301 TGCGCCCAAG CCTTTCAAAC CCCCGTCCAT TCCTTTCAGG CAAAACAGTT 351 TTTTGAACGC TATTTCACGC CGTGGCAGGT TGCAGGCAAC GGAAGCCTTG 401 CCGGTACGGT TACCGGCTAT TACGAACCGG TGCTGAAGGG CGACGACAGG 451 CGGACGGCAC AAGCCCGCTT CCCGATTTAC GGTATTCCCG ACGATTTTAT 501 CTCCGTCCCC CTGCCTGCCG GTTTGCGGAG CGGAAAAGCC CTTGTCCGCA 551 TCAGGCAGAC GGGAAAAAAC AGCGGCACAA TCGACAATAC CGGCGGCACA 601 CATACCGCCG ACCTCTCCCG ATTCCCCATC ACCGCGCGCA CAACAGCAAT 651 CAAAGGCAGG TTTGAAGGAA GCCGCTTCCT CCCCTACCAC ACGCGCAACC 701 AAATCAACGG CGGCGCGCTT GACGGCAAAG CCCCGATACT CGGTTACGCC 751 GAAGACCCTG TCGAACTTTT TTTTATGCAC ATCCAAGGCT CGGGCCGTCT 801 GAAAACCCCG TCCGGCAAAT ACATCCGCAT CGGCTATGCC GACAAAAACG 851 AACATCCCTA CGTTTCCATC GGACGCTATA TGGCGGATAA GGGCTACCTC 901 AAACTCGGAC AAACCTCCAT GCAGGGCATT AAGTCTTATA TGCGGCAAAA 951 TCCGCAACGC CTCGCCGAAG TTTTGGGTCA AAACCCCAGC TATATCTTTT1001 TCCGCGAGCT TGCCGGAAGC AGCAATGACG GCCCTGTCGG CGCACTGGGC1051 ACGCCGCTGA TGGGGGAATA TGCCGGCGCA GTCGACCGGC ACTACATTAC1101 CTTGGGTGCG CCCTTATTTG TCGCCACCGC CCATCCGGTT ACCCGCAAAG1151 CCCTCAACCG CCTGATTATG GCGCAGGATA CCGGCAGCGC GATTAAAGGC1201 GCGGTGCGCG TGGATTATTT TTGGGGATAC GGCGACGAAG CCGGCGAACT1251 TGCCGGCAAA CAGAAAACCA CGGGATATGT CTGGCAGCTC CTACCCAACG1301 GTATGAAGCC CGAATACCGC CCGTAA这对应于氨基酸序列<SEQ ID 35;ORF 919-2>:m919-2.pep  1 MKKYLFRAAL YGIAAAILAA CQSKSIQTFP QPDTSVINGP DRPVGIPDPA 51 GTTVGGGGAV YTVVPHLSLP HWAAQDFAKS LQSFRLGCAN LKNRQGWQDV101 CAQAFQTPVH SFQAKQFFER YFTPWQVAGN GSLAGTVTGY YEPVLKGDDR151 RTAQARFPIY GIPDDFISVP LPAGLRSGKA LVRIRQTGKN SGTIDNTGGT201 HTADLSRFPI TARTTAIKGR FEGSRFLPYH TRNQINGGAL DGKAPILGYA251 EDPVELFFMH IQGSGRLKTP SGKYIRIGYA DKNEHPYVSI GRYMADKGYL301 KLGQTSMQGI KSYMRQNPQR LAEVLGQNPS YIFFRELAGS SNDGPVGALG351 TPLMGEYAGA VDRHYITLGA PLFVATAHPV TRKALNRLIM AQDTGSAIKG401 AVRVDYFWGY GDEAGELAGK QKTTGYVWQL LPNGMKPEYR P*下列部分DNA序列是在淋病奈瑟球菌中鉴别出的<SEQ ID 36>:g919.seq  1 ATGAAAAAAC ACCTGCTCCG CTCCGCCCTG TACGGcatCG CCGCCgccAT 51 CctcgCCGCC TGCCAAAgca gGAGCATCCA AACCTTTCCG CAACCCGACA101 CATCCGTCAT CAACGGCCCG GACCGGCCGG CCGGCATCCC CGACCCCGCC151 GGAACGACGG TTGCCGGCGG CGGGGCCGTC TATACCGTTG TGCCGCACCT201 GTCCATGCCC CACTGGGCGG CGCaggATTT TGCCAAAAGC CTGCAATCCT251 TCCGCCTCGG CTGCGCCAAT TTGAAAAACC GCCAAGGCTG GCAGGATGTG301 TGCGCCCAAG CCTTTCAAAC CCCCGTGCAT TCCTTTCAGG CAAAGcGgTT351 TTTTGAACGC TATTTCACGC cgtGGCaggt tgcaggCaAC GGAAGcCTTG401 Caggtacggt TACCGGCTAT TACGAACCGG TGCTGAAGGG CGACGGCAGG451 CGGACGGAAC GGGCCCGCTT CCCGATTTAC GGTATTCCCG ACGATTTTAT

 501 CTCCGTCCCG CTGCCTGCCG GTTTGCGGGG CGGAAAAAAC CTTGTCCGCA 551 TCAGGCAGac ggGGAAAAAC AGCGGCACGA TCGACAATGC CGGCGGCACG 601 CATACCGCCG ACCTCTCCCG ATTCCCCATC ACCGCGCGCA CAACGGcaat 651 caaaGGCAGG TTTGAaggAA GCCGCTTCCT CCCTTACCAC ACGCGCAACC 701 AAAtcaacGG CGGCgcgcTT GACGGCAAag cccCCATCCT CggttacgcC 751 GAagaccCcG tcgaacttTT TTTCATGCAC AtccaaggCT CGGGCCGCCT 801 GAAAACCCcg tccggcaaat acatCCGCAt cggaTacgcc gacAAAAACG 851 AACAtccgTa tgtttccatc ggACGctaTA TGGCGGACAA AGGCTACCTC 901 AAGctcgggc agACCTCGAT GCAGGgcatc aaagcCTATA TGCGGCAAAA 951 TCCGCAACGC CTCGCCGAAG TTTTGGGTCA AAACCCCAGC TATATCTTTT1001 TCCGCGAGCT TGCCGGAAGC GGCAATGAGG GCCCCGTCGG CGCACTGGGC1051 ACGCCACTGA TGGGGGAATA CGCCGGCGCA ATCGACCGGC ACTACATTAC1101 CTTGGGCGCG CCCTTATTTG TCGCCACCGC CCATCCGGTT ACCCGCAAAG1151 CCCTCAACCG CCTGATTATG GCGCAGGATA CAGGCAGCGC GATCAAAGGC1201 GCGGTGCGCG TGGATTATTT TTGGGGTTAC GGCGACGAAG CCGGCGAACT1251 TGCCGGCAAA CAGAAAACCA CGGGATACGT CTGGCAGCTC CTGCCCAACG1301 GCATGAAGCC CGAATACCGC CCGTGA这对应于氨基酸序列<SEQ ID 37;ORF 919.ng>:g919.pep  1 MKKHLLRSAL YGIAAAILAA CQSRSIQTFP QPDTSVINGP DRPAGIPDPA 51 GTTVAGGGAV YTVVPHLSMP HWAAQDFAKS LQSFRLGCAN LKNRQGWQDV101 CAQAFQTPVH SFQAKRFFER YFTPWQVAGN GSLAGTVTGY YEPVLKGDGR151 RTERARFPIY GIPDDFISVP LPAGLRGGKN LVRIRQTGKN SGTIDNAGGT201 HTADLSRFPI TARTTAIKGR FEGSRFLPYH TRNQINGGAL DGKAPILGYA251 EDPVELFFMH IQGSGRLKTP SGKYIRIGYA DKNEHPYVSI GRYMADKGYL301 KLGQTSMQGI KAYMRQNPQR LAEVLGQNPS YIFFRELAGS GNEGPVGALG351 TPLMGEYAGA IDRHYITLGA PLFVATAHPV TRKALNRLIM AQDTGSAIKG401 AVRVDYFWGY GDEAGELAGK QKTTGYVWQL LPNGMKPEYR P*ORF 919与淋病奈瑟球菌中预测的ORF(ORF 919.ng)有95.9%相同性(在441个氨基酸的重迭区):m919/g919                 10       20      30       40      50       60m919.pep    MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV          |||:|:|:|||||||||||||||:|||||||||||||||||||:||||||||||:|||||g919       MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVINGPDRPAGIPDPAGTTVAGGGAV                 10       20      30       40      50       60                 70       80      90      100     110      120m919.pep    YTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER          ||||||||:|||||||||||||||||||||||||||||||||||||||||||||||:|||g919       YTVVPHLSMPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKRFFER                 70       80      90      100     110      120                130      140     150      160     170      180m919.pep    YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA          |||||||||||||||||||||||||||| |||| :|||||||||||||||||||||:|||g919       YFTPWQVAGNGSLAGTVTGYYEPVLKGDGRRTERARFPIYGIPDDFISVPLPAGLRGGKN                130      140     150      160     170      180                190      200     210      220     230      240m919.pep    LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL          ||||||||||||||||:|||||||||||||||||||||||||||||||||||||||||||g919       LVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL                190      200     210      220     230      240                250      260     270      280     290      300m919.pep    DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||g919       DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL                250      260     270      280     290      300                310      320     330      340     350      360m919.pep    KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA          |||||||||||:||||||||||||||||||||||||||||:|:|||||||||||||||||g919       KLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFFRELAGSGNEGPVGALGTPLMGEYAGA                310      320     330      340     350      360                370      380     390      400     410      420

m919.pep    VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK          :|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||g919       IDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK                370      380     390      400      410      420                430      440m919.pep    QKTTGYVWQLLPNGMKPEYRPX          ||||||||||||||||||||||g919       QKTTGYVWQLLPNGMKPEYRPX                430      440下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 38>:a919.seq   1 ATGAAAAAAT ACCTATTCCG CGCCGCCCTG TGCGGCATCG CCGCCGCCAT  51 CCTCGCCGCC TGCCAAAGCA AGAGCATCCA AACCTTTCCG CAACCCGACA 101 CATCCGTCAT CAACGGCCCG GACCGGCCGG TCGGCATCCC CGACCCCGCC 151 GGAACGACGG TCGGCGGCGG CGGGGCCGTT TATACCGTTG TGCCGCACCT 201 GTCCCTGCCC CACTGGGCGG CGCAGGATTT CGCCAAAAGC CTGCAATCCT 251 TCCGCCTCGG CTGCGCCAAT TTGAAAAACC GCCAAGGCTG GCAGGATGTG 301 TGCGCCCAAG CCTTTCAAAC CCCCGTCCAT TCCGTTCAGG CAAAACAGTT 351 TTTTGAACGC TATTTCACGC CGTGGCAGGT TGCAGGCAAC GGAAGCCTTG 401 CCGGTACGGT TACCGGCTAT TACGAGCCGG TGCTGAAGGG CGACGACAGG 451 CGGACGGCAC AAGCCCGCTT CCCGATTTAC GGTATTCCCG ACGATTTTAT 501 CTCCGTCCCC CTGCCTGCCG GTTTGCGGAG CGGAAAAGCC CTTGTCCGCA 551 TCAGGCAGAC GGGAAAAAAC AGCGGCACAA TCGACAATAC CGGCGGCACA 601 CATACCGCCG ACCTCTCCCA ATTCCCCATC ACTGCGCGCA CAACGGCAAT 651 CAAAGGCAGG TTTGAAGGAA GCCGCTTCCT CCCCTACCAC ACGCGCAACC 701 AAATCAACGG CGGCGCGCTT GACGGCAAAG CCCCGATACT CGGTTACGCC 751 GAAGACCCCG TCGAACTTTT TTTTATGCAC ATCCAAGGCT CGGGCCGTCT 801 GAAAACCCCG TCCGGCAAAT ACATCCGCAT CGGCTATGCC GACAAAAACG 851 AACATCCCTA CGTTTCCATC GGACGCTATA TGGCGGACAA AGGCTACCTC 901 AAGCTCGGGC AGACCTCGAT GCAGGGCATC AAAGCCTATA TGCAGCAAAA 951 CCCGCAACGC CTCGCCGAAG TTTTGGGGCA AAACCCCAGC TATATCTTTT1001 TCCGAGAGCT TACCGGAAGC AGCAATGACG GCCCTGTCGG CGCACTGGGC1051 ACGCCGCTGA TGGGCGAGTA CGCCGGCGCA GTCGACCGGC ACTACATTAC1101 CTTGGGCGCG CCCTTATTTG TCGCCACCGC CCATCCGGTT ACCCGCAAAG1151 CCCTCAACCG CCTGATTATG GCGCAGGATA CCGGCAGCGC GATTAAAGGC1201 GCGGTGCGCG TGGATTATTT TTGGGGATAC GGCGACGAAG CCGGCGAACT1251 TGCCGGCAAA CAGAAAACCA CGGGATATGT CTGGCAGCTT CTGCCCAACG1301 GTATGAAGCC CGAATACCGC CCGTAA这对应于氨基酸序列<SEQ ID 39;ORF 919.a>:a919.pep  1 MKKYLFRAAL CGIAAAILAA CQSKSIQTFP QPDTSVINGP DRPVGIPDPA 51 GTTVGGGGAV YTVVPHLSLP HWAAQDFAKS LQSFRLGCAN LKNRQGWQDV101 CAQAFQTPVH SVQAKQFFER YFTPWQVAGN GSLAGTVTGY YEPVLKGDDR151 RTAQARFPIY GIPDDFISVP LPAGLRSGKA LVRIRQTGKN SGTIDNTGGT201 HTADLSQFPI TARTTAIKGR FEGSRFLPYH TRNQINGGAL DGKAPILGYA251 EDPVELFFMH IQGSGRLKTP SGKYIRIGYA DKNEHPYVSI GRYMADKGYL301 KLGQTSMQGI KAYMQQNPQR LAEVLGQNPS YIFFRELTGS SNDGPVGALG351 TPLMGEYAGA VDRHYITLGA PLFVATAHPV TRKALNRLIM AQDTGSAIKG401 AVRVDYFWGY GDEAGELAGK QKTTGYVWQL LPNGMKPEYR P*m919/a919 ORFs 919和919.a有98.6%相同性(在441个氨基酸的重迭区)                 10       20      30       40      50       60m919.pep    MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV          ||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||a919       MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV                 10       20      30       40      50       60                 70       80      90      100     110      120m919.pep    YTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER          ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||a919       YTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSVQAKQFFER                 70       80      90      100     110      120                130      140     150      160     170      180m919.pep    YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||a919       YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA

                130      140      150      160     170      180                190      200      210      220     230      240m919.pep    LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL          |||||||||||||||||||||||||||:||||||||||||||||||||||||||||||||a919       LVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL                190      200      210      220     230      240                250      260      270      280     290      300m919.pep    DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||a919       DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL                250      260      270      280     290      300                310      320      330      340     350      360m919.pep    KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA          |||||||||||:||:||||||||||||||||||||||:||||||||||||||||||||||a919       KLGQTSMQGIKAYMQQNPQRLAEVLGQNPSYIFFRELTGSSNDGPVGALGTPLMGEYAGA                310      320      330      340     350      360                370      380      390      400     410      420m919.pep    VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||a919       VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK                370      380      390      400     410      420                430      440m919.pep    QKTTGYVWQLLPNGMKPEYRPX          ||||||||||||||||||||||a919       QKTTGYVWQLLPNGMKPEYRPX                430      440121和121-1下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 40>:m121.seq   1 ATGGAAACAC AGCTTTACAT CGGCATCATG TCGGGAACCA GCATGGACGG  51 GGCGGATGCC GTACTGATAC GGATGGACGG CGGCAAATGG CTGGGCGCGG 101 AAGGGCACGC CTTTACCCCC TACCCCGGCA GGTTACGCCG CCAATTGCTG 151 GATTTGCAGG ACACAGGCGC AGACGAACTG CACCGCAGCA GGATTTTGTC 201 GCAAGAACTC AGCCGCCTAT ATGCGCAAAC CGCCGCCGAA CTGCTGTGCA 251 GTCAAAACCT CGCACCGTCC GACATTACCG CCCTCGGCTG CCACGGGCAA 301 ACCGTCCGAC ACGCGCCGGA ACACGGTTAC AGCATACAGC TTGCCGATTT 351 GCCGCTGCTG GCGxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx 401 xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx 451 xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx 501 xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx 551 xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx 601 xxxxxxCAGC TTCCTTACGA CAAAAACGGT GCAAAGTCGG CACAAGGCAA 651 CATATTGCCG CAACTGCTCG ACAGGCTGCT CGCCCACCCG TATTTCGCAC 701 AACGCCACCC TAAAAGCACG GGGCGCGAAC TGTTTGCCAT AAATTGGCTC 751 GAAACCTACC TTGACGGCGG CGAAAACCGA TACGACGTAT TGCGGACGCT 801 TTCCCGTTTT ACCGCGCAA  CCGTTTGCGA CGCCGTCTCA CACGCAGCGG 851 CAGATGCCCG TCAAATGTAC ATTTGCGACG GCGGCATCCG CAATCCTGTT 901 TTAATGGCGG ATTTGGCAGA ATGTTTCGGC ACACGCGTTT CCCTGCACAG 951 CACCGCCGAC CTGAACCTCG ATCCGCAATG GGTGGAAGCC GCCGnATTTG1001 CGTGGTTGGC GGCGTGTTGG ATTAATCGCA TTCCCGGTAG TCCGCACAA1051 GCAACCGGCG CATCCAA CC GTGTATTCTG AnCGCGGGAT ATTATTATTG1101 A这对应于氨基酸序列<SEQ ID 41;ORF 121>:

m121.pep  1 METQLYIGIM SGTSMDGADA VLIRMDGGKW LGAEGHAFTP YPGRLRRQLL 51 DLQDTGADEL HRSRILSQEL SRLYAQTAAE LLCSQNLAPS DITALGCHGQ101 TVRHAPEHGY SIQLADLPLL Axxxxxxxxx xxxxxxxxxx xxxxxxxxxx151 xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx201 xxQLPYDKNG AKSAQGNILP QLLDRLLAHP YFAQRHPKST GRELFAINWL251 ETYLDGGENR YDVLRTLSRF TAQTVCDAVS HAAADARQMY ICDGGIRNPV301 LMADLAECFG TRVSLHSTAD LNLDPQWVEA AXFAWLAACW INRIPGSPHK351 ATGASKPCIL XAGYYY*下列部分DNA序列是在淋病奈瑟球菌中鉴别出的<SEQ ID 42>:g121.seq   1 ATGGAAACAC AGCTTTACAT CGGCATTATG TCGGGAACCA GTATGGACGG  51 GGCGGATGCC GTGCTGGTAC GGATGGACGG CGGCAAATGG CTGGGCGCGG 101 AAGGGCACGC CTTTACCCCC TACCCTGACC GGTTGCGCCG CAAATTGCTG 151 GATTTGCAGG ACACAGGCAC AGACGAACTG CACCGCAGCA GGATGTTGTC 201 GCAAGAACTC AGCCGCCTGT ACGCGCAAAC CGCCGCCGAA CTGCTGTGCA 251 GTCAAAACCT CGCTCCGTGC GACATTACCG CCCTCGGCTG CCACGGGCAA 301 ACCGTCCGAC ACGCGCCGGA ACACGGTtac AGCATACAGC TTGCCGATTT 351 GCCGCTGCTG GCGGAACTGa cgcggatttT TACCGTCggc gacttcCGCA 401 GCCGCGACCT TGCTGCCGGC GGacaAGGTG CGCCGCTCGT CCCCGCCTTT 451 CACGAAGCCC TGTTCCGCGA TGACAGGGAA ACACGCGTGG TACTGAACAT 501 CGGCGGGATT GCCAACATCA GCGTACTCCC CCCCGGCGCA CCCGCCTTCG 551 GCTTCGACAC AGGGCCGGGC AATATGCTGA TGGAcgcgtg gacgcaggca 601 cacTGGcagc TGCCTTACGA CAAAAacggt gcAAAGgcgg cacAAGGCAA 651 catatTGCcg cAACTGCTCG gcaggctGCT CGCCcaccCG TATTTCTCAC 701 AACCCcaccc aaAAAGCACG GGgcGCGaac TgtttgcccT AAattggctc 751 gaaacctAcc ttgacggcgg cgaaaaccga tacgacgtat tgcggacgct 801 ttcccgattc accgcgcaaA ccgTttggga cgccgtctca CACGCAGCGG 851 CAGATGCCCG TCAAATGTAC ATTTGCGGCG GCGGCATCCG CAATCCTGTT 901 TTAATGGCGG ATTTGGCAGA ATGTTTCGGC ACACGCGTTT CCCTGCACAG 951 CACCGCCGAA CTGAACCTCG ATCCTCAATG GGTGGAGGCG gccgCATTtg1001 cgtggttggC GGCGTGTTGG ATTAACCGCA TTCCCGGTAG TCCGCACAAA1051 GCGACCGGCG CATCCAAACC GTGTATTCTG GGCGCGGGAT ATTATTATTG1101 A这对应于氨基酸序列<SEQ ID 43;ORF 121.ng>:g121.pep  1 METQLYIGIM SGTSMDGADA VLVRMDGGKW LGAEGHAFTP YPDRLRRKLL 51 DLQDTGTDEL HRSRMLSQEL SRLYAQTAAE LLCSQNLAPC DITALGCHGQ101 TVRHAPEHGY SIQLADLPLL AELTRIFTVG DFRSRDLAAG GQGAPLVPAF151 HEALFRDDRE TRVVLNIGGI ANISVLPPGA PAFGFDTGPG NMLMDAWTQA201 HWQLPYDKNG AKAAQGNILP QLLGRLLAHP YFSQPHPKST GRELFALNWL251 ETYLDGGENR YDVLRTLSRF TAQTVWDAVS HAAADARQMY ICGGGIRNPV301 LMADLAECFG TRVSLHSTAE LNLDPQWVEA AAFAWLAACW INRIPGSPHK351 ATGASKPCIL GAGYYY*ORF 121与淋病奈瑟球菌中预测的ORF(ORF 121.ng)有73.5%相同性(在366个氨基酸的重迭区).

m121/g121                 10       20      30       40      50       60m121.pep    METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRQLLDLQDTGADEL          ||||||||||||||||||||||:||||||||||||||||||| |||||:||||||||:|||g121       METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDRLRRKLLDLQDTGTDEL                 10       20      30       40      50       60                 70       80      90      100     110      120m121.pep    HRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTVRHAPEHGYSIQLADLPLL          |||||:|||||||||||||||||||||||| ||||||||||||||||||||||||||||||g121       HRSRMLSQELSRLYAQTAAELLCSQNLAPCDITALGCHGQTVRHAPEHGYSIQLADLPLL                 70       80      90      100     110      120                130      140     150      160     170      180m121.pep    AXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

           |  :  :                           :

g121        AELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRETRVVLNIGGIANISVLPPGA                 130      140      150     160      170      180                 190      200      210     220      230      240m121.pep    XXXXXXXXXXXXXXXXXXXXXXQLPYDKNGAKSAQGNILPQLLDRLLAHPYFAQRHPKST               :         :   ||||||||||:|||||||||| ||||||||:| |||||g121       PAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNILPQLLGRLLAHPYFSQPHPKST                 190      200      210     220      230      240                 250      260      270     280      290      300m121.pep    GRELFAINWLETYLDGGENRYDVLRTLSRFTAQTVCDAVSHAAADARQMYICDGGIRNPV          ||||||:||||||||||||||||||||||||||||| |||||||||||||||| |||||||g121       GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVWDAVSHAAADARQMYICGGGIRNPV                 250      260      270     280      290      300                 310      320      330     340      350      360m121.pep    LMADLAECFGTRVSLHSTADLNLDPQWVEAAXFAWLAACWINRIPGSPHKATGASKPCIL          ||||||||||||||||||||:||||||||||| ||||||||||||||||||||||||||||g121       LMADLAECFGTRVSLHSTAELNLDPQWVEAAAFAWLAACWINRIPGSPHKATGASKPCIL                 310      320      330     340      350      360m121.pep    XAGYYYX           ||||||g121       GAGYYYX下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 44>:a121.seq   1 ATGGAAACAC AGCTTTACAT CGGCATCATG TCGGGAACCA GCATGGACGG  51 GGCGGATGCC GTACTGATAC GGATGGACGG CGGCAAATGG CTGGGCGCGG 101 AAGGGCACGC CTTTACCCCC TACCCCGGCA GGTTACGCCG CAAATTGCTG 151 GATTTGCAGG ACACAGGCGC GGACGAACTG CACCGCAGCA GGATGTTGTC 201 GCAAGAACTC AGCCGCCTGT ACGCGCAAAC CGCCGCCGAA CTGCTGTGCA 251 GTCAAAACCT CGCGCCGTCC GACATTACCG CCCTCGGCTG CCACGGGCAA 301 ACCGTCAGAC ACGCGCCGGA ACACAGTTAC AGCGTACAGC TTGCCGATTT 351 GCCGCTGCTG GCGGAACGGA CTCAGATTTT TACCGTCGGC GACTTCCGCA 401 GCCGCGACCT TGCGGCCGGC GGACAAGGCG CGCCGCTCGT CCCCGCCTTT 451 CACGAAGCCC TGTTCCGCGA CGACAGGGAA ACACGCGCGG TACTGAACAT 501 CGGCGGGATT GCCAACATCA GCGTACTCCC CCCCGACGCA CCCGCCTTCG 551 GCTTCGACAC AGGACCGGGC AATATGCTGA TGGACGCGTG GATGCAGGCA 601 CACTGGCAGC TTCCTTACGA CAAAAACGGT GCAAAGGCGG CACAAGGCAA 651 CATATTGCCG CAACTGCTCG ACAGGCTGCT CGCCCACCCG TATTTCGCAC 701 AACCCCACCC TAAAAGCACG GGGCGCGAAC TGTTTGCCCT AAATTGGCTC 751 GAAACCTACC TTGACGGCGG CGAAAACCGA TACGACGTAT TGCGGACGCT 801 TTCCCGATTC ACCGCGCAAA CCGTTTTCGA CGCCGTCTCA CACGCAGCGG 851 CAGATGCCCG TCAAATGTAC ATTTGCGGCG GCGGCATCCG CAATCCTGTT 901 TTAATGGCGG ATTTGGCAGA ATGTTTCGGC ACACGCGTTT CCCTGCACAG 951 CACCGCCGAA CTGAACCTCG ATCCGCAATG GGTAGAAGCC GCCGCGTTCG1001 CATGGATGGC GGCGTGTTGG GTCAACCGCA TTCCCGGTAG TCCGCACAAA1051 GCAACCGGCG CATCCAAACC GTGTATTCTG GGCGCGGGAT ATTATTATTG1101 A这对应于氨基酸序列<SEQ ID 45;ORF 121.a>:a121.pep  1 METQLYIGIM SGTSMDGADA VLIRMDGGKW LGAEGHAFTP YPGRLRRKLL 51 DLQDTGADEL HRSRMLSQEL SRLYAQTAAE LLCSQNLAPS DITALGCHGQ101 TVRHAPEHSY SVQLADLPLL AERTQIFTVG DFRSRDLAAG GQGAPLVPAF151 HEALFRDDRE TRAVLNIGGI ANISVLPPDA PAFGFDTGPG NMLMDAWMQA201 HWQLPYDKNG AKAAQGNILP QLLDRLLAHP YFAQPHPKST GRELFALNWL251 ETYLDGGENR YDVLRTLSRF TAQTVFDAVS HAAADARQMY ICGGGIRNPV301 LMADLAECFG TRVSLHSTAE LNLDPQWVEA AAFAWMAACW VNRIPGSPHK351 ATGASKPCIL GAGYYY*m121/a121 ORFs 121和121.a 74.0%相同性(在366个氨基酸的重迭区)

                 10       20      30       40       50      60m121.pep   METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRQLLDLQDTGADEL         ||||||||||||||||||||||||||||||||||||||||||||||||:||||||||||||a121      METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRKLLDLQDTGADEL                 10       20      30       40       50      60                 70       80      90      100      110     120m121.pep    HRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTVRHAPEHGYSIQLADLPLL          ||||:|||||||||||||||||||||||||||||||||||||||||||:||:||||||||a121       HRSRMLSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTVRHAPEHSYSVQLADLPLL                 70       80       90     100      110     120                130      140      150     160      170     180m121.pep    AXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX          |  :   :                         :

a121       AERTQIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRETRAVLNIGGIANISVLPPDA                130      140      150     160      170     180                190      200      210     220      230     240m121.pep    XXXXXXXXXXXXXXXXXXXXXXQLPYDKNGAKSAQGNILPQLLDRLLAHPYFAQRHPKST               :             ||||||||||:||||||||||||||||||||| |||||a121       PAFGFDTGPGNMLMDAWMQAHWQLPYDKNGAKAAQGNILPQLLDRLLAHPYFAQPHPKST                190      200      210     220      230     240                250      260      270     280      290     300m121.pep    GRELFAINWLETYLDGGENRYDVLRTLSRFTAQTVCDAVSHAAADARQMYICDGGIRNPV          ||||||:|||||||||||||||||||||||||||| ||||||||||||||||| ||||||a121       GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVFDAVSHAAADARQMYICGGGIRNPV                250      260      270     280      290     300                310      320      330     340      350     360m121.pep    LMADLAECFGTRVSLHSTADLNLDPQWVEAAXFAWLAACWINRIPGSPHKATGASKPCIL          ||||||||||||||||||||:||||||||||| |||:||||:||||||||||||||||||a121       LMADLAECFGTRVSLHSTAELNLDPQWVEAAAFAWMAACWVNRIPGSPHKATGASKPCIL                310      320      330     340      350     360m121.pep    XAGYYYX           ||||||a121        GAGYYYX进一步工作揭示,下列DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 46>:m121-1.seq   1 ATGGAAACAC AGCTTTACAT CGGCATCATG TCGGGAACCA GCATGGACGG  51 GGCGGATGCC GTACTGATAC GGATGGACGG CGGCAAATGG CTGGGCGCGG 101 AAGGGCACGC CTTTACCCCC TACCCCGGCA GGTTACGCCG CCAATTGCTG 151 GATTTGCAGG ACACAGGCGC AGACGAACTG CACCGCAGCA GGATTTTGTC 201 GCAAGAACTC AGCCGCCTAT ATGCGCAAAC CGCCGCCGAA CTGCTGTGCA 251 GTCAAAACCT CGCACCGTCC GACATTACCG CCCTCGGCTG CCACGGGCAA 301 ACCGTCCGAC ACGCGCCGGA ACACGGTTAC AGCATACAGC TTGCCGATTT 351 GCCGCTGCTG GCGGAACGGA CGCGGATTTT TACCGTCGGC GACTTCCGCA 401 GCCGCGACCT TGCGGCCGGC GGACAAGGCG CGCCACTCGT CCCCGCCTTT 451 CACGAAGCCC TGTTCCGCGA CAACAGGGAA ACACGCGCGG TACTGAACAT 501 CGGCGGGATT GCCAACATCA GCGTACTCCC CCCCGACGCA CCCGCCTTCG 551 GCTTCGACAC AGGGCCGGGC AATATGCTGA TGGACGCGTG GACGCAGGCA 601 CACTGGCAGC TTCCTTACGA CAAAAACGGT GCAAAGGCGG CACAAGGCAA 651 CATATTGCCG CAACTGCTCG ACAGGCTGCT CGCCCACCCG TATTTCGCAC 701 AACCCCACCC TAAAAGCACG GGGCGCGAAC TGTTTGCCCT AAATTGGCTC 751 GAAACCTACC TTGACGGCGG CGAAAACCGA TACGACGTAT TGCGGACGCT 801 TTCCCGTTTT ACCGCGCAAA CCGTTTGCGA CGCCGTCTCA CACGCAGCGG 851 CAGATGCCCG TCAAATGTAC ATTTGCGGCG GCGGCATCCG CAATCCTGTT 901 TTAATGGCGG ATTTGGCAGA ATGTTTCGGC ACACGCGTTT CCCTGCACAG 951 CACCGCCGAC CTGAACCTCG ATCCGCAATG GGTGGAAGCC GCCGNATTTG1001 CGTGGTTGGC GGCGTGTTGG ATTAATCGCA TTCCCGGTAG TCCGCACAAA1051 GCAACCGGCG CATCCAAACC GTGTATTCTG ANCGCGGGAT ATTATTATTG1101 A这对应于氨基酸序列<SEQ ID 47;ORF 121-1>:

m121-1.pep  1 METQLYIGIM SGTSMDGADA VLIRMDGGKW LGAEGHAFTP YPGRLRRQLL 51 DLQDTGADEL HRSRILSQEL SRLYAQTAAE LLCSQNLAPS DITALGCHGQ101 TVRHAPEHGY SIQLADLPLL AERTRIFTVG DFRSRDLAAG GQGAPLVPAF151 HEALFRDNRE TRAVLNIGGI ANISVLPPDA PAFGFDTGPG NMLMDAWTQA201 HWQLPYDKNG AKAAQGNILP QLLDRLLAHP YFAQPHPKST GRELFALNWL251 ETYLDGGENR YDVLRTLSRF TAQTVCDAVS HAAADARQMY ICGGGIRNPV301 LMADLAECFG TRVSLHSTAD LNLDPQWVEA AXFAWLAACW INRIPGSPHK351 ATGASKPCIL XAGYYY*m121-1/g121 ORFs 121-1和121-1.ng有95.6%相同性(在366个氨基酸的重迭区)                   10      20       30      40       50      60m121-1.pep    METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRQLLDLQDTGADEL            ||||||||||||||||||||||:||||||||||||||||||| ||||:||||||||:|||g121         METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDRLRRKLLDLQDTGTDEL                   10      20       30      40       50      60                   70      80       90     100      110     120m121-1.pep    HRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTVRHAPEHGYSIQLADLPLL            ||||:|||||||||||||||||||||||| ||||||||||||||||||||||||||||||g121         HRSRMLSQELSRLYAQTAAELLCSQNLAPCDITALGCHGQTVRHAPEHGYSIQLADLPLL                   70      80       90     100      110     120                  130     140      150     160      170     180m121-1.pep    AERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRETRAVLNIGGIANISVLPPDA            || ||||||||||||||||||||||||||||||||||:||||:||||||||||||||| |g121         AELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRETRVVLNIGGIANISVLPPGA                  130     140      150     160      170     180                  190     200      210     220      230     240m121-1.pep    PAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNILPQLLDRLLAHPYFAQPHPKST            ||||||||||||||||||||||||||||||||||||||||||| ||||||||:|||||||g121         PAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNILPQLLGRLLAHPYFSQPHPKST                  190      200     210     220      230     240                  250      260     270     280      290     300m121-1.pep    GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVCDAVSHAAADARQMYICGGGIRNPV            ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||g121         GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVWDAVSHAAADARQMYICGGGIRNPV                  250      260     270     280      290     300                  310      320     330     340      350     360m121-1.pep    LMADLAECFGTRVSLHSTADLNLDPQWVEAAXFAWLAACWINRIPGSPHKATGASKPCIL            |||||||||||||||||||:||||||||||| |||||||||||||||||||||||||||||g121         LMADLAECFGTRVSLHSTAELNLDPQWVEAAAFAWLAACWINRIPGSPHKATGASKPCIL                  310      320     330     340      350     360m121-1.pep    XAGYYYX             ||||||g121          GAGYYYX下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 48>:a121-1.seq  1 ATGGAAACAC AGCTTTACAT CGGCATCATG TCGGGAACCA GCATGGACGG 51 GGCGGATGCC GTACTGATAC GGATGGACGG CGGCAAATGG CTGGGCGCGG101 AAGGGCACGC CTTTACCCCC TACCCCGGCA GGTTACGCCG CAAATTGCTG151 GATTTGCAGG ACACAGGCGC GGACGAACTG CACCGCAGCA GGATGTTGTC201 GCAAGAACTC AGCCGCCTGT ACGCGCAAAC CGCCGCCGAA CTGCTGTGCA251 GTCAAAACCT CGCGCCGTCC GACATTACCG CCCTCGGCTG CCACGGGCAA301 ACCGTCAGAC ACGCGCCGGA ACACAGTTAC AGCGTACAGC TTGCCGATTT351 GCCGCTGCTG GCGGAACGGA CTCAGATTTT TACCGTCGGC GACTTCCGCA401 GCCGCGACCT TGCGGCCGGC GGACAAGGCG CGCCGCTCGT CCCCGCCTTT451 CACGAAGCCC TGTTCCGCGA CGACAGGGAA ACACGCGCGG TACTGAACAT501 CGGCGGGATT GCCAACATCA GCGTACTCCC CCCCGACGCA CCCGCCTTCG551 GCTTCGACAC AGGACCGGGC AATATGCTGA TGGACGCGTG GATGCAGGCA601 CACTGGCAGC TTCCTTACGA CAAAAACGGT GCAAAGGCGG CACAAGGCAA651 CATATTGCCG CAACTGCTCG ACAGGCTGCT CGCCCACCCG TATTTCGCAC701 AACCCCACCC TAAAAGCACG GGGCGCGAAC TGTTTGCCCT AAATTGGCTC

 751 GAAACCTACC TTGACGGCGG CGAAAACCGA TACGACGTAT TGCGGACGCT 801 TTCCCGATTC ACCGCGCAAA CCGTTTTCGA CGCCGTCTCA CACGCAGCGG 851 CAGATGCCCG TCAAATGTAC ATTTGCGGCG GCGGCATCCG CAATCCTGTT 901 TTAATGGCGG ATTTGGCAGA ATGTTTCGGC ACACGCGTTT CCCTGCACAG 951 CACCGCCGAA CTGAACCTCG ATCCGCAATG GGTAGAAGCC GCCGCGTTCG1001 CATGGATGGC GGCGTGTTGG GTCAACCGCA TTCCCGGTAG TCCGCACAAA1051 GCAACCGGCG CATCCAAACC GTGTATTCTG GGCGCGGGAT ATTATTATTG1101 A这对应于氨基酸序列<SEQ ID 49;ORF 121-1.a>:a121-1.pep  1 METQLYIGIM SGTSMDGADA VLIRMDGGKW LGAEGHAFTP YPGRLRRKLL 51 DLQDTGADEL HRSRMLSQEL SRLYAQTAAE LLCSQNLAPS DITALGCHGQ101 TVRHAPEHSY SVQLADLPLL AERTQIFTVG DFRSRDLAAG GQGAPLVPAF151 HEALFRDDRE TRAVLNIGGI ANISVLPPDA PAFGFDTGPG NMLMDAWMQA201 HWQLPYDKNG AKAAQGNILP QLLDRLLAHP YFAQPHPKST GRELFALNWL251 ETYLDGGENR YDVLRTLSRF TAQTVFDAVS HAAADARQMY ICGGGIRNPV301 LMADLAECFG TRVSLHSTAE LNLDPQWVEA AAFAWMAACW VNRIPGSPHK351 ATGASKPCIL GAGYYY*m121-1/a121-1 ORFs 121-1和121-1.a有96.4%相同性(在366个氨基酸的重迭区)                   10       20      30      40       50       60m121-1.pep    METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRQLLDLQDTGADEL            |||||||||||||||||||||||||||||||||||||||||||||||:||||||||||||a121-1       METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRKLLDLQDTGADEL                   10       20      30      40       50       60                   70       80      90     100      110      120m121-1.pep    HRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTVRHAPEHGYSIQLADLPLL            ||||:|||||||||||||||||||||||||||||||||||||||||||:||:||||||||a121-1       HRSRMLSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTVRHAPEHSYSVQLADLPLL                   70       80      90     100      110      120                  130      140     150     160      170      180m121-1.pep    AERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRETRAVLNIGGIANISVLPPDA            ||||:||||||||||||||||||||||||||||||||:||||||||||||||||||||||a121-1       AERTQIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRETRAVLNIGGIANISVLPPDA                  130      140     150     160      170      180                  190      200     210     220      230      240m121-1.pep    PAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNILPQLLDRLLAHPYFAQPHPKST            ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||a121-1       PAFGFDTGPGNMLMDAWMQAHWQLPYDKNGAKAAQGNILPQLLDRLLAHPYFAQPHPKST                  190      200     210     220      230      240                  250      260     270     280      290      300m121-1.pep    GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVCDAVSHAAADARQMYICGGGIRNPV            ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||a121-1       GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVFDAVSHAAADARQMYICGGGIRNPV                  250      260     270     280      290      300                  310      320     330     340      350      360m121-1.pep    LMADLAECFGTRVSLHSTADLNLDPQWVEAAXFAWLAACWINRIPGSPHKATGASKPCIL            |||||||||||||||||||:|||||||||||| |||:|||:|||||||||||||||||||a121         LMADLAECFGTRVSLHSTAELNLDPQWVEAAAFAWMAACWVNRIPGSPHKATGASKPCIL                  310      320     330     340      350      360m121-1.pep    XAGYYYX             ||||||a121         GAGYYYX128和128-1下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 50>:

m128.seq(部分)   1 ATGACTGACA ACGCACTGCT CCATTTGGGC GAAGAACCCC GTTTTGATCA  51 AATCAAAACC GAAGACATCA AACCCGCCCT GCAAACCGCC ATCGCCGAAG 101 CGCGCGAACA AATCGCCGCC ATCAAAGCCC AAACGCACAC CGGCTGGGCA 151 AACACTGTCG AACCCCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTGGTG TCGCACCTCA ACTGCGTCGC CGACACGCCC GAACTGCGCG 251 CCGTCTATAA CGAACTGATG CCCGAAATCA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAGCTGTA CAACCGCTTC AAAACCATCA AAAATTCCCC 351 CGAATTCGAC ACCCTCTCCC CCGCACAAAA AACCAAACTC AACCAC   1 TACGCCAGCG AAAAACTGCG CGAAGCCAAA TACGCGTTCA GCGAAACCGA  51 wGTCAAAAAA TAyTTCCCyG TCGGCAAwGT ATTAAACGGA CTGTTCGCCC 101 AAmTCAAAAA ACTmTACGGC ATCGGATTTA CCGAAAAAAC yGTCCCCGTC 151 TGGCACAAAG ACGTGCGCTA TTkTGAATTG CAACAAAACG GCGAAmCCAT 201 AGGCGGCGTT TATATGGATT TGTACGCACG CGAAGGCAAA CGCGGCGGCG 251 CGTGGATGAA CGACTACAAA GGCCGCCGCC GTTTTTCAGA CGGCACGCTG 301 CAAyTGCCCA CCGCCTACCT CGTCTGCAAC TTCGCCCCAC CCGTCGGCGG 351 CAGGGAAGCC CGCyTGAGCC ACGACGAAAT CCTCATCCTC TTCCACGAAA 401 CCGGACACGG GCTGCACCAC CTGCTTACCC AAGTGGACGA ACTGGGCGTA 451 TCCGGCATCA ACGGCGTAkA ATGGGACGCG GTCGAACTGC CCAGCCAGTT 501 TATGGAAAAT TTCGTTTGGG AATACAATGT CTTGGCACAA mTGTCAGCCC 551 ACGAAGAAAC CGGcgTTCCC yTGCCGAAAG AACTCTTsGA CAAAwTGCTC 601 GCCGCCAAAA ACTTCCAAsG CGGCATGTTC yTsGTCCGGC AAwTGGAGTT 651 CGCCCTCTTT GATATGATGA TTTACAGCGA AGACGACGAA GGCCGTCTGA 701 AAAACTGGCA ACAGGTTTTA GACAGCGTGC GCAAAAAAGT CGCCGTCATC 751 CAGCCGCCCG AATACAACCG CTTCGCCTTG AGCTTCGGCC ACATCTTCGC 801 AGGCGGCTAT TCCGCAGCTn ATTACAGCTA CGCGTGGGCG GAAGTATTGA 851 GCGCGGACGC ATACGCCGCC TTTGAAGAAA GCGACGATGT CGCCGCCACA 901 GGCAAACGCT TTTGGCAGGA AATCCTCGCC GTCGGGGnAT CGCGCAGCGG 951 nGCAGAATCC TTCAAAGCCT TCCGCGGCCG CGAACCGAGC ATAGACGCAC1001 TCTTGCGCCA CAGCGGTTTC GACAACGCGG TCTGA这对应于氨基酸序列<SEQ ID 51;ORF 128>:m128.pep(部分)  1 MTDNALLHLG EEPRFDQIKT EDIKPALQTA IAEAREQIAA IKAQTHTGWA 51 NTVEPLTGIT ERVGRIWGVV SHLNCVADTP ELRAVYNELM PEITVFFTEI101 GQDIELYNRF KTIKNSPEFD TLSPAQKTKL NH//  1 YASEKLREAK YAFSETXVKK YFPVGXVLNG LFAQXKKLYG IGFTEKTVPV 51 WHKDVRYXEL QQNGEXIGGV YMDLYAREGK RGGAWMNDYK GRRRFSDGTL101 QLPTAYLVCN FAPPVGGREA RLSHDEILIL FHETGHGLHH LLTQVDELGV151 SGINGVXWDA VELPSQFMEN FVWEYNVLAQ XSAHEETGVP LPKELXDKXL201 AAKNFQXGMF XVRQXEFALF DMMIYSEDDE GRLKNWQQVL DSVRKKVAVI251 QPPEYNRFAL SFGHIFAGGY SAAXYSYAWA EVLSADAYAA FEESDDVAAT301 GKRFWQEILA VGXSRSGAES FKAFRGREPS IDALLRHSGF DNAV*下列部分DNA序列是在淋病奈瑟球菌中鉴别出的<SEQ ID 52>:g128.seq  1 atgattgaca acgCActgct ccacttgggc gaagaaccCC GTTTTaatca 51 aatccaaacc gaagACAtca AACCCGCCGT CCAAACCGCC ATCGCCGAAG101 CGCGCGGACA AATCGCCGCC GTCAAAGCGC AAACGCACAC CGGCTGGGCG151 AACACCGTCG AGCGTCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG201 GGGCGTCGTG TCCCATCTCA ACTCCGTCGT CGACACGCCC GAACTGCGCG251 CCGTCTATAA CGAACTGATG CCTGAAATCA CCGTCTTCTT CACCGAAATC301 GGACAAGACA TCGAACTGTA CAACCGCTTC AAAACCATCA AAAATTCCCC351 CGAATTTGCA ACGCTTTCCC CCGCACAAAA AACCAAGCTC GATCACGACC401 TGCGCGATTT CGTATTGAGC GGCGCGGAAC TGCCGCCCGA ACGGCAGGCA451 GAACTGGCAA AACTGCAAAC CGAAGGCGCG CAACTTTCCG CCAAATTCTC501 CCAAAACGTC CTAGACGCGA CCGACGCGTT CGGCATTTAC TTTGACGATG551 CCGCACCGCT TGCCGGCATT CCCGAAGACG CGCTCGCCAT GTTTGCCGCC601 GCCGCGCAAA GCGAAGGCAA AACAGGTTAC AAAATCGGCT TGCAGATTCC651 GCACTACCTT GCCGTTATCC AATACGCCGG CAACCGCGAA CTGCGCGAAC701 AAATCTACCG CGCCTACGTT ACCCGTGCCA GCGAACTTTC AAACGACGGC751 AAATTCGACA ACACCGCCAA CATCGACCGC ACGCTCGAAA ACGCATTGAA801 AACCGccaaa cTGCTCGGCT TTAAAAATTA CGCCGAATTG TCGCTGGCAA851 CCAAAATGGC GGACACGCCC GAACAGGTTT TAAACTTCCT GCACGACCTC901 GCCCGCCGCG CCAAACCCTA CGCCGAAAAA GACCTCGCCG AAGTCAAAGC951 CTTCGCCCGC GAACACCTCG GTCTCGCCGA CCCGCAGCCG TGGGACTTGA

1001 GCTACGCCGG CGAAAAACTG CGCGAAGCCA AATACGCATT CAGCGAAACC1051 GAAGTCAAAA AATACTTCCC CGTCGGCAAA GTTCTGGCAG GCCTGTTCGC1101 CCAAATCAAA AAACTCTACG GCATCGGATT CGCCGAAAAA ACCGTTCCCG1151 TCTGGCACAA AGACGTGCGC TATTTTGAAT TGCAACAAAA CGGCAAAACC1201 ATCGGCGGCG TTTATATGGA TTTGTACGCA CGCGAAGGCA AACGCGGCGG1251 CGCGTGGATG AACGACtaca AAGGCCGCCG CCGCTTTGCC GACGgcacGC1301 TGCAACTGCC CACCGCCTAC CTCGTCTGCA ACTTCGCCCC GCCCGTCGGC1351 GGCAAAGAAG CGCGTTTAAG CCACGACGAA ATCCTCACCC TCTTCCACGA1401 AacCGGCCAC GGACTGCACC ACCTGCTTAC CCAAGTGGAC GAACTGGGCG1451 TGTCCGGCAT CAAcggcgtA GAATGGGACG CGGTCGAACT GCCCAGCCAG1501 TTTATGGAAA ACTTCGTTTG GGAATACAAT GTATTGGCAC AAATGTCCGC1551 CCACGAAGAA AccgGCGAGC CCCTGCCGAA AGAACTCTTC GACAAAATGC1601 TcgcCGCCAA AAACTTCCAG CGCGGTATGT TCCTCGTCCG GCAAATGGAG1651 TTCGCCCTCT TCGATATGAT GATTTACAGT GAAAGCGACG AATGCCGTCT1701 GAAAAACTGG CAGCAGGTTT TAGACAGCGT GCGCAAAGAA GTCGCCGTCA1751 TCCAACCGCC CGAATACAAC CGCTTCGCCA ACAGCTTCGG CCacatctTC1801 GCcggcGGCT ATTCCGCAGG CTATTACAGC TACGCATGGG CCGAAGTCCt1851 cAGCACCGAT GCCTACGCCG CCTTTGAAGA AAGcGACGac gtcGCCGCCA1901 CAGGCAAACG CTTCTGGCAA GAAAtccttg ccgtcggcgg ctCCCGCAGC1951 gcgGCGGAAT CCTTCAAAGC CTTCCGCGGA CGCGAACCGA GCATAGACGC2001 ACTGCTGCGC CAaagcggtT TCGACAACGC gGCttgA这对应于氨基酸序列<SEQ ID 53;ORF 128.ng>:g128.pep  1 MIDNALLHLG EEPRFNQIQT EDIKPAVQTA IAEARGQIAA VKAQTHTGWA 51 NTVERLTGIT ERVGRIWGVV SHLNSVVDTP ELRAVYNELM PEITVFFTEI101 GQDIELYNRF KTIKNSPEFA TLSPAQKTKL DHDLRDFVLS GAELPPERQA151 ELAKLQTEGA QLSAKFSQNV LDATDAFGIY FDDAAPLAGI PEDALAMFAA201 AAQSEGKTGY KIGLQIPHYL AVIQYAGNRE LREQIYRAYV TRASELSNDG251 KFDNTANIDR TLENALKTAK LLGFKNYAEL SLATKMADTP EQVLNFLHDL301 ARRAKPYAEK DLAEVKAFAR EHLGLADPQP WDLSYAGEKL REAKYAFSET351 EVKKYFPVGK VLAGLFAQIK KLYGIGFAEK TVPVWHKDVR YFELQQNGKT401 IGGVYMDLYA REGKRGGAWM NDYKGRRRFA DGTLQLPTAY LVCNFAPPVG451 GKEARLSHDE ILTLFHETGH GLHHLLTQVD ELGVSGINGV EWDAVELPSQ501 FMENFVWEYN VLAQMSAHEE TGEPLPKELF DKMLAAKNFQ RGMFLVRQME551 FALFDMMIYS ESDECRLKNW QQVLDSVRKE VAVIQPPEYN RFANSFGHIF601 AGGYSAGYYS YAWAEVLSTD AYAAFEESDD VAATGKRFWQ EILAVGGSRS651 AAESFKAFRG REPSIDALLR QSGFDNAA*ORF 128与淋病奈瑟球菌中预测的ORF(ORF 128.ng)有91.7%相同性(在475个氨基酸的重迭区):m128/g128                 10       20      30       40      50       60g128.pep    MIDNALLHLGEEPRFNQIQTEDIKPAVQTAIAEARGQIAAVKAQTHTGWANTVERLTGIT          | ||||||||||||||:||:|||||||:|||||||| ||||:|||||||||||| |||||m128       MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT                 10       20      30       40      50       60                 70       80      90      100     110      120g128.pep    ERVGRIWGVVSHLNSVVDTPELRAVYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFA          |||||||||||||| |:|||||||||||||||||||||||||||||||||||||||||||m128       ERVGRIWGVVSHLNCVADTPELRAVYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD                 70       80      90      100     110      120                130      140     150      160     170      180g128.pep    TLSPAQKTKLDHDLRDFVLSGAELPPERQAELAKLQTEGAQLSAKFSQNVLDATDAFGIY          ||||||||||:|m128       TLSPAQKTKLNH                130                   //                                       340      350      360g128.pep                              YAGEKLREAKYAFSETEVKKYFPVGKVLAG                                    ||:||||||||||||| ||||||| || |m128                                 YASEKLREAKYAFSETXVKKYFPVGXVLNG                                           10      20       30             370      380      390     400      410      420g128.pep    LFAQIKKLYGIGFAEKTVPVWHKDVRYFELQQNGKTIGGVYMDLYAREGKRGGAWMNDYK           |||| ||||||||:|||||||||||| |||||||::|||||||||||||||||||||||

m128        LFAQXKKLYGIGFTEKTVPVWHKDVRYXELQQNGEXIGGVYMDLYAREGKRGGAWMNDYK                  40      50       60      70       80       90             430      440      450     460      470      480g128.pep    GRRRFADGTLQLPTAYLVCNFAPPVGGKEARLSHDEILTLFHETGHGLHHLLTQVDELGV          |||||:||||||||||||||||||||||:||||||||| |||||||||||||||||||||m128       GRRRFSDGTLQLPTAYLVCNFAPPVGGREARLSHDEILILFHETGHGLHHLLTQVDELGV                100      110      120     130      140      150             490      500      510     520      530      540g128.pep    SGINGVEWDAVELPSQFMENFVWEYNVLAQMSAHEETGEPLPKELFDKMLAAKNFQRGMF          |||||| ||||||||||||||||||||||| ||||||| ||||||| || ||||||| |||m128       SGINGVXWDAVELPSQFMENFVWEYNVLAQXSAHEETGVPLPKELXDKXLAAKNFQXGMF                160      170     180      190      200      210             550      560      570      580     590      600g128.pep    LVRQMEFALFDMMIYSESDECRLKNWQQVLDSVRKEVAVIQPPEYNRFANSFGHIFAGGY           ||| ||||||||||||:|| ||||||||||||||:||||||||||||| ||||||||||m128        XVRQXEFALFDMMIYSEDDEGRLKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGY                 220      230     240      250      260      270             610      620      630      640     650      660g128.pep    SAGYYSYAWAEVLSTDAYAAFEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPS          ||:  ||||||||||:|||||||||||||||||||||||||| ||||:||||||||||||m128       SAAXYSYAWAEVLSADAYAAFEESDDVAATGKRFWQEILAVGXSRSGAESFKAFRGREPS                280      290     300      310      320      330             670     679g128.pep    IDALLRQSGFDNAAX          ||||||:||||||:

m128       IDALLRHSGFDNAVX                340下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 54>:a128.seq   1 ATGACTGACA ACGCACTGCT CCATTTGGGC GAAGAACCCC GTTTTGATCA  51 AATCAAAACC GAAGACATCA AACCCGCCCT GCAAACCGCC ATTGCCGAAG 101 CGCGCGAACA AATCGCCGCC ATCAAAGCCC AAACGCACAC CGGCTGGGCA 151 AACACTGTCG AACCCCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTGGTG TCGCACCTCA ACTCCGTCAC CGACACGCCC GAACTGCGCG 251 CCGCCTACAA TGAATTAATG CCCGAAATTA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAGCTGTA CAACCGCTTC AAAACCATCA AAAACTCCCC 351 CGAGTTCGAC ACCCTCTCCC ACGCGCAAAA AACCAAACTC AACCACGATC 401 TGCGCGATTT CGTCCTCAGC GGCGCGGAAC TGCCGCCCGA ACAGCAGGCA 451 GAATTGGCAA AACTGCAAAC CGAAGGCGCG CAACTTTCCG CCAAATTCTC 501 CCAAAACGTC CTAGACGCGA CCGACGCGTT CGGCATTTAC TTTGACGATG 551 CCGCACCGCT TGCCGGCATT CCCGAAGACG CGCTCGCCAT GTTTGCCGCT 601 GCCGCGCAAA GCGAAGGCAA AACAGGCTAC AAAATCGGTT TGCAGATTCC 651 GCACTACCTC GCCGTCATCC AATACGCCGA CAACCGCAAA CTGCGCGAAC 701 AAATCTACCG CGCCTACGTT ACCCGCGCCA GCGAGCTTTC AGACGACGGC 751 AAATTCGACA ACACCGCCAA CATCGACCGC ACGCTCGAAA ACGCCCTGCA 801 AACCGCCAAA CTGCTCGGCT TCAAAAACTA CGCCGAATTG TCGCTGGCAA 851 CCAAAATGGC GGACACCCCC GAACAAGTTT TAAACTTCCT GCACGACCTC 901 GCCCGCCGCG CCAAACCCTA CGCCGAAAAA GACCTCGCCG AAGTCAAAGC 951 CTTCGCCCGC GAAAGCCTCG GCCTCGCCGA TTTGCAACCG TGGGACTTGG1001 GCTACGCCGG CGAAAAACTG CGCGAAGCCA AATACGCATT CAGCGAAACC1051 GAAGTCAAAA AATACTTCCC CGTCGGCAAA GTATTAAACG GACTGTTCGC1101 CCAAATCAAA AAACTCTACG GCATCGGATT TACCGAAAAA ACCGTCCCCG1151 TCTGGCACAA AGACGTGCGC TATTTTGAAT TGCAACAAAA CGGCGAAACC1201 ATAGGCGGCG TTTATATGGA TTTGTACGCA CGCGAAGGCA AACGCGGCGG1251 CGCGTGGATG AACGACTACA AAGGCCGCCG CCGTTTTTCA GACGGCACGC1301 TGCAACTGCC CACCGCCTAC CTCGTCTGCA ACTTCACCCC GCCCGTCGGC1351 GGCAAAGAAG CCCGCTTGAG CCATGACGAA ATCCTCACCC TCTTCCACGA1401 AACCGGACAC GGCCTGCACC ACCTGCTTAC CCAAGTCGAC GAACTGGGCG1451 TATCCGGCAT CAACGGCGTA GAATGGGACG CAGTCGAACT GCCCAGTCAG1501 TTTATGGAAA ATTTCGTTTG GGAATACAAT GTCTTGGCGC AAATGTCCGC1551 CCACGAAGAA ACCGGCGTTC CCCTGCCGAA AGAACTCTTC GACAAAATGC1601 TCGCCGCCAA AAACTTCCAA CGCGGAATGT TCCTCGTCCG CCAAATGGAG1651 TTCGCCCTCT TTGATATGAT GATTTACAGC GAAGACGACG AAGGCCGTCT1701 GAAAAACTGG CAACAGGTTT TAGACAGCGT GCGCAAAGAA GTCGCCGTCG1751 TCCGACCGCC CGAATACAAC CGCTTCGCCA ACAGCTTCGG CCACATCTTC1801 GCAGGCGGCT ATTCCGCAGG CTATTACAGC TACGCGTGGG CGGAAGTATT

1851 GAGCGCGGAC GCATACGCCG CCTTTGAAGA AAGCGACGAT GTCGCCGCCA1901 CAGGCAAACG CTTTTGGCAG GAAATCCTCG CCGTCGGCGG ATCGCGCAGC1951 GCGGCAGAAT CCTTCAAAGC CTTCCGCGGA CGCGAACCGA GCATAGACGC2001 ACTCTTGCGC CACAGCGGCT TCGACAACGC GGCTTGA这对应于氨基酸序列<SEQ ID 55;ORF 128.a>:a128.pep  1 MTDNALLHLG EEPRFDQIKT EDIKPALQTA IAEAREQIAA IKAQTHTGWA 51 NTVEPLTGIT ERVGRIWGVV SHLNSVTDTP ELRAAYNELM PEITVFFTEI101 GQDIELYNRF KTIKNSPEFD TLSHAQKTKL NHDLRDFVLS GAELPPEQQA151 ELAKLQTEGA QLSAKFSQNV LDATDAFGIY FDDAAPLAGI PEDALAMFAA201 AAQSEGKTGY KIGLQIPHYL AVIQYADNRK LREQIYRAYV TRASELSDDG251 KFDNTANIDR TLENALQTAK LLGFKNYAEL SLATKMADTP EQVLNFLHDL301 ARRAKPYAEK DLAEVKAFAR ESLGLADLQP WDLGYAGEKL REAKYAFSET351 EVKKYFPVGK VLNGLFAQIK KLYGIGFTEK TVPVWHKDVR YFELQQNGET401 IGGVYMDLYA REGKRGGAWM NDYKGRRRFS DGTLQLPTAY LVCNFTPPVG451 GKEARLSHDE ILTLFHETGH GLHHLLTQVD ELGVSGINGV EWDAVELPSQ501 FMENFVWEYN VLAQMSAHEE TGVPLPKELF DKMLAAKNFQ RGMFLVRQME551 FALFDMMIYS EDDEGRLKNW QQVLDSVRKE VAVVRPPEYN RFANSFGHIF601 AGGYSAGYYS YAWAEVLSAD AYAAFEESDD VAATGKRFWQ EILAVGGSRS651 AAESFKAFRG REPSIDALLR HSGFDNAA*m128/a128 ORFs 128和128.a有66.0%相同性(在677个氨基酸的重迭区)                 10       20      30       40      50       60m128.pep    MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||a128       MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT                 10       20      30       40      50       60                 70       80      90      100     110      120m128.pep    ERVGRIWGVVSHLNCVADTPELRAVYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD          |||||||||||||| |:|||||||:|||||||||||||||||||||||||||||||||||a128       ERVGRIWGVVSHLNSVTDTPELRAAYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD                 70       80      90      100     110      120                130m128.pep    TLSPAQKTKLNH------------------------------------------------          ||| ||||||||a128       TLSHAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGAQLSAKFSQNVLDATDAFGIY                130      140     150      160     170      180m128.pep    -----------------------------------------------------------a128        FDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPHYLAVIQYADNRKLREQIYRAYV                190      200     210      220     230      240m128.pep    -----------------------------------------------------------a128        TRASELSDDGKFDNTANIDRTLENALQTAKLLGFKNYAELSLATKMADTPEQVLNFLHDL                250      260     270      280      290     300                                          140      150m128.pep    ---------------------------------YASEKLREAKYAFSETXVKKYFPVGX                                      ||:|||||||||||| |||||||||a128        ARRAKPYAEKDLAEVKAFARESLGLADLQPWDLGYAGEKLREAKYAFSETEVKKYFPVGK                310      320     330      340      350     360          160      170     180      190      200     210m128.pep    VLNGLFAQXKKLYGIGFTEKTVPVWHKDVRYXELQQNGEXIGGVYMDLYAREGKRGGAWM          |||||||| |||||||||||||||||||||| |||||||:||||||||||||||||||||a128       VLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYFELQQNGETIGGVYMDLYAREGKRGGAWM                370      380    390       400      410     420          220      230     240      250      260     270m128.pep    NDYKGRRRFSDGTLQLPTAYLVCNFAPPVGGREARLSHDEILILFHETGHGLHHLLTQVD          |||||||||||||||||||||||||:|||||:|||||||||| |||||||||||||||||a128       NDYKGRRRFSDGTLQLPTAYLVCNFTPPVGGKEARLSHDEILTLFHETGHGLHHLLTQVD                430      440    450       460      470     480          280      290     300      310      320     330m128.pep    ELGVSGINGVXWDAVELPSQFMENFVWEYNVLAQXSAHEETGVPLPKELXDKXLAAKNFQ

           |||||||||| ||||||||||||||||||||||| |||||||||||||| || ||||||a128        ELGVSGINGVEWDAVELPSQFMENFVWEYNVLAQMSAHEETGVPLPKELFDKMLAAKNFQ                 490      500      510     520      530      540          340      350      360     370      380      390m128.pep    XGMFXVRQXEFALFDMMIYSEDDEGRLKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIF           ||| ||| ||||||||||||||||||||||||||||||:|||::|||||||| ||||||a128       RGMFLVRQMEFALFDMMIYSEDDEGRLKNWQQVLDSVRKEVAVVRPPEYNRFANSFGHIF                550      560      570     580      590      600          400      410     420      430      440      450m128.pep    AGGYSAAXYSYAWAEVLSADAYAAFEESDDVAATGKRFWQEILAVGXSRSGAESFKAFRG          ||||||:  |||||||||||||||||||||||||||||||||||||| |||:|||||||||a128       AGGYSAGYYSYAWAEVLSADAYAAFEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRG                610      620      630      640     650      660          460      470m128.pep    REPSIDALLRHSGFDNAVX           |||||||||||||||||:

a128       REPSIDALLRHSGFDNAAX                670进一步工作揭示,下列DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 56>:m128-1.seq   1 ATGACTGACA ACGCACTGCT CCATTTGGGC GAAGAACCCC GTTTTGATCA  51 AATCAAAACC GAAGACATCA AACCCGCCCT GCAAACCGCC ATCGCCGAAG 101 CGCGCGAACA AATCGCCGCC ATCAAAGCCC AAACGCACAC CGGCTGGGCA 151 AACACTGTCG AACCCCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTGGTG TCGCACCTCA ACTCCGTCGC CGACACGCCC GAACTGCGCG 251 CCGTCTATAA CGAACTGATG CCCGAAATCA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAGCTGTA CAACCGCTTC AAAACCATCA AAAATTCCCC 351 CGAATTCGAC ACCCTCTCCC CCGCACAAAA AACCAAACTC AACCACGATC 401 TGCGCGATTT CGTCCTCAGC GGCGCGGAAC TGCCGCCCGA ACAGCAGGCA 451 GAACTGGCAA AACTGCAAAC CGAAGGCGCG CAACTTTCCG CCAAATTCTC 501 CCAAAACGTC CTAGACGCGA CCGACGCGTT CGGCATTTAC TTTGACGATG 551 CCGCACCGCT TGCCGGCATT CCCGAAGACG CGCTCGCCAT GTTTGCCGCC 601 GCCGCGCAAA GCGAAAGCAA AACAGGCTAC AAAATCGGCT TGCAGATTCC 651 ACACTACCTC GCCGTCATCC AATACGCCGA CAACCGCGAA CTGCGCGAAC 701 AAATCTACCG CGCCTACGTT ACCCGCGCCA GCGAACTTTC AGACGACGGC 751 AAATTCGACA ACACCGCCAA CATCGACCGC ACGCTCGCAA ACGCCCTGCA 801 AACCGCCAAA CTGCTCGGCT TCAAAAACTA CGCCGAATTG TCGCTGGCAA 851 CCAAAATGGC GGACACGCCC GAACAAGTTT TAAACTTCCT GCACGACCTC 901 GCCCGCCGCG CCAAACCCTA CGCCGAAAAA GACCTCGCCG AAGTCAAAGC 951 CTTCGCCCGC GAAAGCCTGA ACCTCGCCGA TTTGCAACCG TGGGACTTGG1001 GCTACGCCAG CGAAAAACTG CGCGAAGCCA AATACGCGTT CAGCGAAACC1051 GAAGTCAAAA AATACTTCCC CGTCGGCAAA GTATTAAACG GACTGTTCGC1101 CCAAATCAAA AAACTCTACG GCATCGGATT TACCGAAAAA ACCGTCCCCG1151 TCTGGCACAA AGACGTGCGC TATTTTGAAT TGCAACAAAA CGGCGAAACC1201 ATAGGCGGCG TTTATATGGA TTTGTACGCA CGCGAAGGCA AACGCGGCGG1251 CGCGTGGATG AACGACTACA AAGGCCGCCG CCGTTTTTCA GACGGCACGC1301 TGCAACTGCC CACCGCCTAC CTCGTCTGCA ACTTCGCCCC ACCCGTCGGC1351 GGCAGGGAAG CCCGCCTGAG CCACGACGAA ATCCTCATCC TCTTCCACGA1401 AACCGGACAC GGGCTGCACC ACCTGCTTAC CCAAGTGGAC GAACTGGGCG1451 TATCCGGCAT CAACGGCGTA GAATGGGACG CGGTCGAACT GCCCAGCCAG1501 TTTATGGAAA ATTTCGTTTG GGAATACAAT GTCTTGGCAC AAATGTCAGC1551 CCACGAAGAA ACCGGCGTTC CCCTGCCGAA AGAACTCTTC GACAAAATGC1601 TCGCCGCCAA AAACTTCCAA CGCGGCATGT TCCTCGTCCG GCAAATGGAG1651 TTCGCCCTCT TTGATATGAT GATTTACAGC GAAGACGACG AAGGCCGTCT1701 GAAAAACTGG CAACAGGTTT TAGACAGCGT GCGCAAAAAA GTCGCCGTCA1751 TCCAGCCGCC CGAATACAAC CGCTTCGCCT TGAGCTTCGG CCACATCTTC1801 GCAGGCGGCT ATTCCGCAGG CTATTACAGC TACGCGTGGG CGGAAGTATT1851 GAGCGCGGAC GCATACGCCG CCTTTGAAGA AAGCGACGAT GTCGCCGCCA1901 CAGGCAAACG CTTTTGGCAG GAAATCCTCG CCGTCGGCGG ATCGCGCAGC1951 GCGGCAGAAT CCTTCAAAGC CTTCCGCGGC CGCGAACCGA GCATAGACGC2001 ACTCTTGCGC CACAGCGGTT TCGACAACGC GGTCTGA这对应于氨基酸序列<SEQ ID 57;ORF 128-1>:

m128-1.pep.

  1 MTDNALLHLG EEPRFDQIKT EDIKPALQTA IAEAREQIAA IKAQTHTGWA 51 NTVEPLTGIT ERVGRIWGVV SHLNSVADTP ELRAVYNELM PEITVFFTEI101 GQDIELYNRF KTIKNSPEFD TLSPAQKTKL NHDLRDFVLS GAELPPEQQA151 ELAKLQTEGA QLSAKFSQNV LDATDAFGIY FDDAAPLAGI PEDALAMFAA201 AAQSESKTGY KIGLQIPHYL AVIQYADNRE LREQIYRAYV TRASELSDDG251 KFDNTANIDR TLANALQTAK LLGFKNYAEL SLATKMADTP EQVLNFLHDL301 ARRAKPYAEK DLAEVKAFAR ESLNLADLQP WDLGYASEKL REAKYAFSET351 EVKKYFPVGK VLNGLFAQIK KLYGIGFTEK TVPVWHKDVR YFELQQNGET401 IGGVYMDLYA REGKRGGAWM NDYKGRRRFS DGTLQLPTAY LVCNFAPPVG451 GREARLSHDE ILILFHETGH GLHHLLTQVD ELGVSGINGV EWDAVELPSQ501 FMENFVWEYN VLAQMSAHEE TGVPLPKELF DKMLAAKNFQ RGMFLVRQME551 FALFDMMIYS EDDEGRLKNW QQVLDSVRKK VAVIQPPEYN RFALSFGHIF601 AGGYSAGYYS YAWAEVLSAD AYAAFEESDD VAATGKRFWQ EILAVGGSRS651 AAESFKAFRG REPSIDALLR HSGFDNAV*下列部分DNA序列是在淋病奈瑟球菌中鉴别出的<SEQ ID 58>:g128-1.seq(部分)   1 ATGATTGACA ACGCACTGCT CCACTTGGGC GAAGAACCCC GTTTTAATCA  51 AATCAAAACC GAAGACATCA AACCCGCCGT CCAAACCGCC ATCGCCGAAG 101 CGCGCGGACA AATCGCCGCC GTCAAAGCGC AAACGCACAC CGGCTGGGCG 151 AACACCGTCG AGCGTCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTCGTG TCCCATCTCA ACTCCGTCGT CGACACGCCC GAACTGCGCG 251 CCGTCTATAA CGAACTGATG CCTGAAATCA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAACTGTA CAACCGCTTC AAAACCATCA AAAATTCCCC 351 CGAATTTGCA ACGCTTTCCC CCGCACAAAA AACCAAGCTC GATCACGACC 401 TGCGCGATTT CGTATTGAGC GGCGCGGAAC TGCCGCCCGA ACGGCAGGCA 451 GAACTGGCAA AACTGCAAAC CGAAGGCGCG CAACTTTCCG CCAAATTCTC 501 CCAAAACGTC CTAGACGCGA CCGACGCGTT CGGCATTTAC TTTGACGATG 551 CCGCACCGCT TGCCGGCATT CCCGAAGACG CGCTCGCCAT GTTTGCCGCC 601 GCCGCGCAAA GCGAAGGCAA AACAGGTTAC AAAATCGGCT TGCAGATTCC 651 GCACTACCTT GCCGTTATCC AATACGCCGG CAACCGCGAA CTGCGCGAAC 701 AAATCTACCG CGCCTACGTT ACCCGTGCCA GCGAACTTTC AAACGACGGC 751 AAATTCGACA ACACCGCCAA CATCGACCGC ACGCTCGAAA ACGCATTGAA 801 AACCGCCAAA CTGCTCGGCT TTAAAAATTA CGCCGAATTG TCGCTGGCAA 851 CCAAAATGGC GGACACGCCC GAACAGGTTT TAAACTTCCT GCACGACCTC 901 GCCCGCCGCG CCAAACCCTA CGCCGAAAAA GACCTCGCCG AAGTCAAAGC 951 CTTCGCCCGC GAACACCTCG GTCTCGCCGA CCCGCAGCCG TGGGACTTGA1001 GCTACGCCGG CGAAAAACTG CGCGAAGCCA AATACGCATT CAGCGAAACC1051 GAAGTCAAAA AATACTTCCC CGTCGGCAAA GTTCTGGCAG GCCTGTTCGC1101 CCAAATCAAA AAACTCTACG GCATCGGATT CGCCGAAAAA ACCGTTCCCG1151 TCTGGCACAA AGACGTGCGC TATTTTGAAT TGCAACAAAA CGGCAAAACC1201 ATCGGCGGCG TTTATATGGA TTTGTACGCA CGCGAAGGCA AACGCGGCGG1251 CGCGTGGATG AACGACTACA AAGGCCGCCG CCGCTTTGCC GACGGCACGC1301 TGCAACTGCC CACCGCCTAC CTCGTCTGCA ACTTCGCCCC GCCCGTCGGC1351 GGCAAAGAAG CGCGTTTAAG CCACGACGAA ATCCTCACCC TCTTCCACGA1401 AACCGGCCAC GGACTGCACC ACCTGCTTAC CCAAGTGGAC GAACTGGGCG1451 TGTCCGGCAT CAACGGCGTA AAA这对应于氨基酸序列<SEQ ID 59;ORF 128-1.ng>:g128-1.pep(部分)  1 MIDNALLHLG EEPRFNQIKT EDIKPAVQTA IAEARGQIAA VKAQTHTGWA 51 NTVERLTGIT ERVGRIWGVV SHLNSVVDTP ELRAVYNELM PEITVFFTEI101 GQDIELYNRF KTIKNSPEFA TLSPAQKTKL DHDLRDFVLS GAELPPERQA151 ELAKLQTEGA QLSAKFSQNV LDATDAFGIY FDDAAPLAGI PEDALAMFAA201 AAQSEGKTGY KIGLQIPHYL AVIQYAGNRE LREQIYRAYV TRASELSNDG251 KFDNTANIDR TLENALKTAK LLGFKNYAEL SLATKMADTP EQVLNFLHDL301 ARRAKPYAEK DLAEVKAFAR EHLGLADPQP WDLSYAGEKL REAKYAFSET351 EVKKYFPVGK VLAGLFAQIK KLYGIGFAEK TVPVWHKDVR YFELQQNGKT401 IGGVYMDLYA REGKRGGAWM NDYKGRRRFA DGTLQLPTAY LVCNFAPPVG451 GKEARLSHDE ILTLFHETGH GLHHLLTQVD ELGVSGINGV Km128-1/g128-1 ORFs 128-1和128-1.ng有94.5%相同性(在491个氨基酸的重迭区)                   10      20       30       40      50       60g128-1.pep    MIDNALLHLGEEPRFNQIKTEDIKPAVQTAIAEARGQIAAVKAQTHTGWANTVERLTGIT            | |||||||||||||:||||||||||:|||||||| ||||:||||||||||||| |||||m128-1       MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT

                   10      20       30       40       50      60                   70      80       90      100      110     120g128-1.pep    ERVGRIWGVVSHLNSVVDTPELRAVYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFA            ||||||||||||||||:||||||||||||||||||||||||||||||||||||||||||m128-1       ERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD                   70      80       90      100      110     120                  130     140      150      160      170     180g128-1.pep    TLSPAQKTKLDHDLRDFVLSGAELPPERQAELAKLQTEGAQLSAKFSQNVLDATDAFGIY            ||||||||||:||||||||||||||||:||||||||||||||||||||||||||||||||m128-1       TLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGAQLSAKFSQNVLDATDAFGIY                  130     140      150      160      170     180                  190     200      210      220      230     240g128-1.pep    FDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPHYLAVIQYAGNRELREQIYRAYV            |||||||||||||||||||||||||:|||||||||||||||||||| |||||||||||||m128-1       FDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPHYLAVIQYADNRELREQIYRAYV                  190     200      210      220      230     240                  250     260      270      280      290     300g128-1.pep    TRASELSNDGKFDNTANIDRTLENALKTAKLLGFKNYAELSLATKMADTPEQVLNFLHDL            |||||||:|||||||||||||| |||:|||||||||||||||||||||||||||||||||m128-1       TRASELSDDGKFDNTANIDRTLANALQTAKLLGFKNYAELSLATKMADTPEQVLNFLHDL                  250     260      270      280      290     300                  310     320      330      340      350     360g128-1.pep    ARRAKPYAEKDLAEVKAFAREHLGLADPQPWDLSYAGEKLREAKYAFSETEVKKYFPVGK            ||||||||||||||||||||| |:||| |||||:||:|||||||||||||||||||||||m128-1       ARRAKPYAEKDLAEVKAFARESLNLADLQPWDLGYASEKLREAKYAFSETEVKKYFPVGK                  310     320      330      340      350     360                  370     380      390      400      410     420g128-1.pep    VLAGLFAQIKKLYGIGFAEKTVPVWHKDVRYFELQQNGKTIGGVYMDLYAREGKRGGAWM            || ||||||||||||||:||||||||||||||||||||:|||||||||||||||||||||m128-1       VLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYFELQQNGETIGGVYMDLYAREGKRGGAWM                  370     380      390      400      410     420                  430     440      450      460      470     480g128-1.pep    NDYKGRRRFADGTLQLPTAYLVCNFAPPVGGKEARLSHDEILTLFHETGHGLHHLLTQVD            |||||||||:|||||||||||||||||||||:|||||||||| |||||||||||||||||m128-1       NDYKGRRRFSDGTLQLPTAYLVCNFAPPVGGREARLSHDEILILFHETGHGLHHLLTQVD                  430     440      450      460      470     480                  490g128-1.pep    ELGVSGINGVK            ||||||||||:

m128-1       ELGVSGINGVEWDAVELPSQFMENFVWEYNVLAQMSAHEETGVPLPKELFDKMLAAKNFQ                  490     500      510      520      530     540下列DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 60>:a128-1.seq  1 ATGACTGACA ACGCACTGCT CCATTTGGGC GAAGAACCCC GTTTTGATCA 51 AATCAAAACC GAAGACATCA AACCCGCCCT GCAAACCGCC ATTGCCGAAG101 CGCGCGAACA AATCGCCGCC ATCAAAGCCC AAACGCACAC CGGCTGGGCA151 AACACTGTCG AACCCCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG201 GGGCGTGGTG TCGCACCTCA ACTCCGTCAC CGACACGCCC GAACTGCGCG251 CCGCCTACAA TGAATTAATG CCCGAAATTA CCGTCTTCTT CACCGAAATC301 GGACAAGACA TCGAGCTGTA CAACCGCTTC AAAACCATCA AAAACTCCCC351 CGAGTTCGAC ACCCTCTCCC ACGCGCAAAA AACCAAACTC AACCACGATC401 TGCGCGATTT CGTCCTCAGC GGCGCGGAAC TGCCGCCCGA ACAGCAGGCA451 GAATTGGCAA AACTGCAAAC CGAAGGCGCG CAACTTTCCG CCAAATTCTC501 CCAAAACGTC CTAGACGCGA CCGACGCGTT CGGCATTTAC TTTGACGATG551 CCGCACCGCT TGCCGGCATT CCCGAAGACG CGCTCGCCAT GTTTGCCGCT601 GCCGCGCAAA GCGAAGGCAA AACAGGCTAC AAAATCGGTT TGCAGATTCC651 GCACTACCTC GCCGTCATCC AATACGCCGA CAACCGCAAA CTGCGCGAAC701 AAATCTACCG CGCCTACGTT ACCCGCGCCA GCGAGCTTTC AGACGACGGC751 AAATTCGACA ACACCGCCAA CATCGACCGC ACGCTCGAAA ACGCCCTGCA801 AACCGCCAAA CTGCTCGGCT TCAAAAACTA CGCCGAATTG TCGCTGGCAA851 CCAAAATGGC GGACACCCCC GAACAAGTTT TAAACTTCCT GCACGACCTC901 GCCCGCCGCG CCAAACCCTA CGCCGAAAAA GACCTCGCCG AAGTCAAAGC951 CTTCGCCCGC GAAAGCCTCG GCCTCGCCGA TTTGCAACCG TGGGACTTGG

1001 GCTACGCCGG CGAAAAACTG CGCGAAGCCA AATACGCATT CAGCGAAACC1051 GAAGTCAAAA AATACTTCCC CGTCGGCAAA GTATTAAACG GACTGTTCGC1101 CCAAATCAAA AAACTCTACG GCATCGGATT TACCGAAAAA ACCGTCCCCG1151 TCTGGCACAA AGACGTGCGC TATTTTGAAT TGCAACAAAA CGGCGAAACC1201 ATAGGCGGCG TTTATATGGA TTTGTACGCA CGCGAAGGCA AACGCGGCGG1251 CGCGTGGATG AACGACTACA AAGGCCGCCG CCGTTTTTCA GACGGCACGC1301 TGCAACTGCC CACCGCCTAC CTCGTCTGCA ACTTCACCCC GCCCGTCGGC1351 GGCAAAGAAG CCCGCTTGAG CCATGACGAA ATCCTCACCC TCTTCCACGA1401 AACCGGACAC GGCCTGCACC ACCTGCTTAC CCAAGTCGAC GAACTGGGCG1451 TATCCGGCAT CAACGGCGTA GAATGGGACG CAGTCGAACT GCCCAGTCAG1501 TTTATGGAAA ATTTCGTTTG GGAATACAAT GTCTTGGCGC AAATGTCCGC1551 CCACGAAGAA ACCGGCGTTC CCCTGCCGAA AGAACTCTTC GACAAAATGC1601 TCGCCGCCAA AAACTTCCAA CGCGGAATGT TCCTCGTCCG CCAAATGGAG1651 TTCGCCCTCT TTGATATGAT GATTTACAGC GAAGACGACG AAGGCCGTCT1701 GAAAAACTGG CAACAGGTTT TAGACAGCGT GCGCAAAGAA GTCGCCGTCG1751 TCCGACCGCC CGAATACAAC CGCTTCGCCA ACAGCTTCGG CCACATCTTC1801 GCAGGCGGCT ATTCCGCAGG CTATTACAGC TACGCGTGGG CGGAAGTATT1851 GAGCGCGGAC GCATACGCCG CCTTTGAAGA AAGCGACGAT GTCGCCGCCA1901 CAGGCAAACG CTTTTGGCAG GAAATCCTCG CCGTCGGCGG ATCGCGCAGC1951 GCGGCAGAAT CCTTCAAAGC CTTCCGCGGA CGCGAACCGA GCATAGACGC2001 ACTCTTGCGC CACAGCGGCT TCGACAACGC GGCTTGA这对应于氨基酸序列<SEQ ID 61;ORF 128-1.a>:a128-1.pep  1 MTDNALLHLG EEPRFDQIKT EDIKPALQTA IAEAREQIAA IKAQTHTGWA 51 NTVEPLTGIT ERVGRIWGVV SHLNSVTDTP ELRAAYNELM PEITVFFTEI101 GQDIELYNRF KTIKNSPEFD TLSHAQKTKL NHDLRDFVLS GAELPPEQQA151 ELAKLQTEGA QLSAKFSQNV LDATDAFGIY FDDAAPLAGI PEDALAMFAA201 AAQSEGKTGY KIGLQIPHYL AVIQYADNRK LREQIYRAYV TRASELSDDG251 KFDNTANIDR TLENALQTAK LLGFKNYAEL SLATKMADTP EQVLNFLHDL301 ARRAKPYAEK DLAEVKAFAR ESLGLADLQP WDLGYAGEKL REAKYAFSET351 EVKKYFPVGK VLNGLFAQIK KLYGIGFTEK TVPVWHKDVR YFELQQNGET401 IGGVYMDLYA REGKRGGAWM NDYKGRRRFS DGTLQLPTAY LVCNFTPPVG451 GKEARLSHDE ILTLFHETGH GLHHLLTQVD ELGVSGINGV EWDAVELPSQ501 FMENFVWEYN VLAQMSAHEE TGVPLPKELF DKMLAAKNFQ RGMFLVRQME551 FALFDMMIYS EDDEGRLKNW QQVLDSVRKE VAVVRPPEYN RFANSFGHIF601 AGGYSAGYYS YAWAEVLSAD AYAAFEESDD VAATGKRFWQ EILAVGGSRS651 AAESFKAFRG REPSIDALLR HSGFDNAA*m128-1/a128-1 ORFs 128-1和128-1.a有97.8%相同性(在677个氨基酸的重迭区)                   10       20      30       40      50       60a128-1.pep    MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m128-1       MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT                   10       20      30       40      50       60                   70       80      90      100     110      120a128-1.pep    ERVGRIWGVVSHLNSVTDTPELRAAYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD            ||||||||||||||||:|||||||:|||||||||||||||||||||||||||||||||||m128-1       ERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD                   70       80      90      100     110      120                  130      140     150      160     170      180a128-1.pep    TLSHAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGAQLSAKFSQNVLDATDAFGIY            ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||m128-1       TLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGAQLSAKFSQNVLDATDAFGIY                  130      140     150      160     170      180                  190      200     210      220     230      240a128-1.pep    FDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPHYLAVIQYADNRKLREQIYRAYV            |||||||||||||||||||||||||:|||||||||||||||||||||||:||||||||||m128-1       FDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPHYLAVIQYADNRELREQIYRAYV                  190      200     210      220     230      240                  250      260     270      280     290      300a128-1.pep    TRASELSDDGKFDNTANIDRTLENALQTAKLLGFKNYAELSLATKMADTPEQVLNFLHDL            |||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||m128-1       TRASELSDDGKFDNTANIDRTLANALQTAKLLGFKNYAELSLATKMADTPEQVLNFLHDL                  250      260     270      280     290      300                  310      320     330      340     350      360a128-1.pep    ARRAKPYAEKDLAEVKAFARESLGLADLQPWDLGYAGEKLREAKYAFSETEVKKYFPVGK

            |||||||||||||||||||||||:||||||||||||:|||||||||||||||||||||||m128-1       ARRAKPYAEKDLAEVKAFARESLNLADLQPWDLGYASEKLREAKYAFSETEVKKYFPVGK                  310      320      330     340      350      360                  370      380      390     400      410      420a128-1.pep    VLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYFELQQNGETIGGVYMDLYAREGKRGGAWM            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m128-1       VLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYFELQQNGETIGGVYMDLYAREGKRGGAWM                  370      380      390     400      410      420                  430      440      450     460      470      480a128-1.pep    NDYKGRRRFSDGTLQLPTAYLVCNFTPPVGGKEARLSHDEILTLFHETGHGLHHLLTQVD            |||||||||||||||||||||||||:|||||:|||||||||| |||||||||||||||||m128-1       NDYKGRRRFSDGTLQLPTAYLVCNFAPPVGGREARLSHDEILILFHETGHGLHHLLTQVD                  430      440      450     460      470      480                  490      500      510     520      530      540a128-1.pep    ELGVSGINGVEWDAVELPSQFMENFVWEYNVLAQMSAHEETGVPLPKELFDKMLAAKNFQ            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m128-1       ELGVSGINGVEWDAVELPSQFMENFVWEYNVLAQMSAHEETGVPLPKELFDKMLAAKNFQ                  490      500      510     520      530      540                  550      560      570     580      590      600a128-1.pep    RGMFLVRQMEFALFDMMIYSEDDEGRLKNWQQVLDSVRKEVAVVRPPEYNRFANSFGHIF            |||||||||||||||||||||||||||||||||||||||:|||::|||||||| ||||||m128-1       RGMFLVRQMEFALFDMMIYSEDDEGRLKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIF                  550      560      570     580      590      600                  610      620      630     640      650      660a128-1.pep    AGGYSAGYYSYAWAEVLSADAYAAFEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRG            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m128-1       AGGYSAGYYSYAWAEVLSADAYAAFEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRG                  610      620      630     640      650      660                  670      679a128-1.pep    REPSIDALLRHSGFDNAAX            |||||||||||||||||:

m128-1       REPSIDALLRHSGFDNAVX                  670206下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 62>:m206.seq  1 ATGTTTCCCC CCGACAAAAC CCTTTTCCTC TGTCTCAGCG CACTGCTCCT 51 CGCCTCATGC GGCACGACCT CCGGCAAACA CCGCCAACCG AAACCCAAAC101 AGACAGTCCG GCAAATCCAA GCCGTCCGCA TCAGCCACAT CGACCGCACA151 CAAGGCTCGC AGGAACTCAT GCTCCACAGC CTCGGACTCA TCGGCACGCC201 CTACAAATGG GGCGGCAGCA GCACCGCAAC CGGCTTCGAT TGCAGCGGCA251 TGATTCAATT CGTTTACAAr AACGCCCTCA ACGTCAAGCT GCCGCGCACC301 GCCCGCGACA TGGCGGCGGC AAGCCGsAAA ATCCCCGAcA GCCGCyTCAA351 GGCCGGCGAC CTCGTATTCT TCAACACCGG CGGCGCACAC CGCTACTCAC401 ACGTCGGACT CTACATCGGC AACGGCGAAT TCATCCATGC CCCCAGCAGC451 GGCAAAACCA TCAAAACCGA AAAACTCTCC ACACCGTTTT ACGCCAAAAA501 CTACCTCGGC GCACATACTT TTTTTACAGA ATGA这对应于氨基酸序列<SEQ ID 63;ORF 206>:m206.pep..

  1 MFPPDKTLFL CLSALLLASC GTTSGKHRQP KPKQTVRQIQ AVRISHIDRT 51 QGSQELMLHS LGLIGTPYKW GGSSTATGFD CSGMIQFVYK NALNVKLPRT101 ARDMAAASRK IPDSRXKAGD LVFFNTGGAH RYSHVGLYIG NGEFIHAPSS151 GKTIKTEKLS TPFYAKNYLG AHTFFTE*下列部分DNA序列是在淋病奈瑟球菌中鉴别出的<SEQ ID 64>:

g206.seq  1 atgttttccc ccgacaaaac ccttttcctc tgtctcggcg cactgctcct 51 cgcctcatgc ggcacgacct ccggcaaaca ccgccaaccg aaacccaaac101 agacagtccg gcaaatccaa gccgtccgca tcagccacat cggccgcaca151 caaggctcgc aggaactcat gctccacagc ctcggactca tcggcacgcc201 ctacaaatgg ggcggcagca gcaccgcaac cggcttcgac tgcagcggca251 tgattcaatt ggtttacaaa aacgccctca acgtcaagct gccgcgcacc301 gcccgcgaca tggcggcggc aagccgcaaa atccccgaca gccgcctcaa351 ggccggcgac atcgtattct tcaacaccgg cggcgcacac cgctactcac401 scgtcggact ctacatcggc aacggcgaat tcatccatgc ccccggcagc451 ggcaaaacca tcaaaaccga aaaactctcc acaccgtttt acgccaaaaa501 ctaccttgga gcgcatacgt tttttacaga atga这对应于氨基酸序列<SEQ ID 65;ORF 206.ng>:g206.pep  1 MFSPDKTLFL CLGALLLASC GTTSGKHRQP KPKQTVRQIQ AVRISHIGRT 51 QGSQELMLHS LGLIGTPYKW GGSSTATGFD CSGMIQLVYK NALNVKLPRT101 ARDMAAASRK IPDSRLKAGD IVFFNTGGAH RYSHVGLYIG NGEFIHAPGS151 GKTIKTEKLS TPFYAKNYLG AHTFFTE*ORF 206与淋病奈瑟球菌中预测的ORF(ORF 206.ng)有96.0%相同性(在177个氨基酸的重迭区):m206/g206                 10       20      30       40       50      60m206.pep    MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRISHIDRTQGSQELMLHS          || |||||||||:|||||||||||||||||||||||||||||||||| ||||||||||||g206       MFSPDKTLFLCLGALLLASCGTTSGKHRQPKPKQTVRQIQAVRISHIGRTQGSQELMLHS                 10       20      30       40       50      60                 70       80      90      100      110     120m206.pep    LGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTARDMAAASRKIPDSRXKAGD          ||||||||||||||||||||||||||:|||||||||||||||||||||||||||| ||||g206       LGLIGTPYKWGGSSTATGFDCSGMIQLVYKNALNVKLPRTARDMAAASRKIPDSRLKAGD                 70       80      90      100      110     120                130      140     150      160      170m206.pep    LVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLSTPFYAKNYLGAHTFFTEX          :|||||||||||||||||||||||||||:|||||||||||||||||||||||||||||g206       IVFFNTGGAHRYSHVGLYIGNGEFIHAPGSGKTIKTEKLSTPFYAKNYLGAHTFFTE                130      140     150      160      170下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 66>:a206.seq  1 ATGTTTCCCC CCGACAAAAC CCTTTTCCTC TGTCTCAGCG CACTGCTCCT 51 CGCCTCATGC GGCACGACCT CCGGCAAACA CCGCCAACCG AAACCCAAAC101 AGACAGTCCG GCAAATCCAA GCCGTCCGCA TCAGCCACAT CGACCGCACA151 CAAGGCTCGC AGGAACTCAT GCTCCACAGC CTCGGACTCA TCGGCACGCC201 CTACAAATGG GGCGGCAGCA GCACCGCAAC CGGCTTCGAT TGCAGCGGCA251 TGATTCAATT CGTTTACAAA AACGCCCTCA ACGTCAAGCT GCCGCGCACC301 GCCCGCGACA TGGCGGCGGC AAGCCGCAAA ATCCCCGACA GCCGCCTTAA351 GGCCGGCGAC CTCGTATTCT TCAACACCGG CGGCGCACAC CGCTACTCAC401 ACGTCGGACT CTATATCGGC AACGGCGAAT TCATCCATGC CCCCAGCAGC451 GGCAAAACCA TCAAAACCGA AAAACTCTCC ACACCGTTTT ACGCCAAAAA501 CTACCTCGGC GCACATACTT TCTTTACAGA ATGA这对应于氨基酸序列<SEQ ID 67;ORF 206.a>:a206.pep  1 MFPPDKTLFL CLSALLLASC GTTSGKHRQP KPKQTVRQIQ AVRISHIDRT 51 QGSQELMLHS LGLIGTPYKW GGSSTATGFD CSGMIQFVYK NALNVKLPRT101 ARDMAAASRK IPDSRLKAGD LVFFNTGGAH RYSHVGLYIG NGEFIHAPSS151 GKTIKTEKLS TPFYAKNYLG AHTFFTE*m206/a206 ORFs 206和206.a有99.4%相同性(在177个氨基酸的重迭区)

                 10       20      30       40      50       60m206.pep    MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRISHIDRTQGSQELMLHS          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||a206       MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRISHIDRTQGSQELMLHS                 10       20      30       40      50       60                 70       80      90      100     110      120m206.pep    LGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTARDMAAASRKIPDSRXKAGD          ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||a206       LGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTARDMAAASRKIPDSRLKAGD                 70       80      90      100     110      120                130      140     150      160     170m206.pep    LVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLSTPFYAKNYLGAHTFFTEX          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||a206       LVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLSTPFYAKNYLGAHTFFTEX                130      140     150      160     170287下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 68>:m287.seq   1 ATGTTTAAAC GCAGCGTAAT CGCAATGGCT TGTATTTTTG CCCTTTCAGC  51 CTGCGGGGGC GGCGGTGGCG GATCGCCCGA TGTCAAGTCG GCGGACACGC 101 TGTCAAAACC TGCCGCCCCT GTTGTTTCTG AAAAAGAGAC AGAGGCAAAG 151 GAAGATGCGC CACAGGCAGG TTCTCAAGGA CAGGGCGCGC CATCCGCACA 201 AGGCAGTCAA GATATGGCGG CGGTTTCGGA AGAAAATACA GGCAATGGCG 251 GTGCGGTAAC AGCGGATAAT CCCAAAAATG AAGACGAGGT GGCACAAAAT 301 GATATGCCGC AAAATGCCGC CGGTACAGAT AGTTCGACAC CGAATCACAC 351 CCCGGATCCG AATATGCTTG CCGGAAATAT GGAAAATCAA GCAACGGATG 401 CCGGGGAATC GTCTCAGCCG GCAAACCAAC CGGATATGGC AAATGCGGCG 451 GACGGAATGC AGGGGGACGA TCCGTCGGCA GGCGGGCAAA ATGCCGGCAA 501 TACGGCTGCC CAAGGTGCAA ATCAAGCCGG AAACAATCAA GCCGCCGGTT 551 CTTCAGATCC CATCCCCGCG TCAAACCCTG CACCTGCGAA TGGCGGTAGC 601 AATTTTGGAA GGGTTGATTT GGCTAATGGC GTTTTGATTG ACGGGCCGTC 651 GCAAAATATA ACGTTGACCC ACTGTAAAGG CGATTCTTGT AGTGGCAATA 701 ATTTCTTGGA TGAAGAAGTA CAGCTAAAAT CAGAATTTGA AAAATTAAGT 751 GATGCAGACA AAATAAGTAA TTACAAGAAA GATGGGAAGA ATGATAAATT 801 TGTCGGTTTG GTTGCCGATA GTGTGCAGAT GAAGGGAATC AATCAATATA 851 TTATCTTTTA TAAACCTAAA CCCACTTCAT TTGCGCGATT TAGGCGTTCT 901 GCACGGTCGA GGCGGTCGCT TCCGGCCGAG ATGCCGCTGA TTCCCGTCAA 951 TCAGGCGGAT ACGCTGATTG TCGATGGGGA AGCGGTCAGC CTGACGGGGC1001 ATTCCGGCAA TATCTTCGCG CCCGAAGGGA ATTACCGGTA TCTGACTTAC1051 GGGGCGGAAA AATTGCCCGG CGGATCGTAT GCCCTTCGTG TTCAAGGCGA1101 ACCGGCAAAA GGCGAAATGC TTGCGGGCGC GGCCGTGTAC AACGGCGAAG1151 TACTGCATTT CCATACGGAA AACGGCCGTC CGTACCCGAC CAGGGGCAGG1201 TTTGCCGCAA AAGTCGATTT CGGCAGCAAA TCTGTGGACG GCATTATCGA1251 CAGCGGCGAT GATTTGCATA TGGGTACGCA AAAATTCAAA GCCGCCATCG1301 ATGGAAACGG CTTTAAGGGG ACTTGGACGG AAAATGGCAG CGGGGATGTT1351 TCCGGAAAGT TTTACGGCCC GGCCGGCGAG GAAGTGGCGG GAAAATACAG1401 CTATCGCCCG ACAGATGCGG AAAAGGGCGG ATTCGGCGTG TTTGCCGGCA1451 AAAAAGAGCA GGATTGA这对应于氨基酸序列<SEQ ID 69;ORF 287>:m287.pep  1 MFKRSVIAMA CIFALSACGG GGGGSPDVKS ADTLSKPAAP VVSEKETEAK 51 EDAPQAGSQG QGAPSAQGSQ DMAAVSEENT GNGGAVTADN PKNEDEVAQN101 DMPQNAAGTD SSTPNHTPDP NMLAGNMENQ ATDAGESSQP ANQPDMANAA151 DGMQGDDPSA GGQNAGNTAA QGANQAGNNQ AAGSSDPIPA SNPAPANGGS201 NFGRVDLANG VLIDGPSQNI TLTHCKGDSC SGNNFLDEEV QLKSEFEKLS251 DADKISNYKK DGKNDKFVGL VADSVQMKGI NQYIIFYKPK PTSFARFRRS301 ARSRRSLPAE MPLIPVNQAD TLIVDGEAVS LTGHSGNIFA PEGNYRYLTY351 GAEKLPGGSY ALRVQGEPAK GEMLAGAAVY NGEVLHFHTE NGRPYPTRGR401 FAAKVDFGSK SVDGIIDSGD DLHMGTQKFK AAIDGNGFKG TWTENGSGDV451 SGKFYGPAGE EVAGKYSYRP TDAEKGGFGV FAGKKEQD*下列部分DNA序列是在淋病奈瑟球菌中鉴别出的<SEQ ID 70>:

g287.seq   1 atgtttaaac gcagtgtgat tgcaatggct tgtatttttc ccctttcagc  51 ctgtgggggc ggcggtggcg gatcgcccga tgtcaagtcg gcggacacgc 101 cgtcaaaacc ggccgccccc gttgttgctg aaaatgccgg ggaaggggtg 151 ctgccgaaag aaaagaaaga tgaggaggca gcgggcggtg cgccgcaagc 201 cgatacgcag gacgcaaccg ccggagaagg cagccaagat atggcggcag 251 tttcggcaga aaatacaggc aatggcggtg cggcaacaac ggacaacccc 301 aaaaatgaag acgcgggggc gcaaaatgat atgccgcaaa atgccgccga 351 atccgcaaat caaacaggga acaaccaacc cgccggttct tcagattccg 401 cccccgcgtc aaaccctgcc cctgcgaatg gcggtagcga ttttggaagg 451 acgaacgtgg gcaattctgt tgtgattgac ggaccgtcgc aaaatataac 501 gttgacccac tgtaaaggcg attcttgtaa tggtgataat ttattggatg 551 aagaagcacc gtcaaaatca gaatttgaaa aattaagtga tgaagaaaaa 601 attaagcgat ataaaaaaga cgagcaacgg gagaattttg tcggtttggt 651 tgctgacagg gtaaaaaagg atggaactaa caaatatatc atcttctata 701 cggacaaacc acctactcgt tctgcacggt cgaggaggtc gcttccggcc 751 gagattccgc tgattcccgt caatcaggcc gatacgctga ttgtggatgg 801 ggaagcggtc agcctgacgg ggcattccgg caatatcttc gcgcccgaag 851 ggaattaccg gtatctgact tacggggcgg aaaaattgcc cggcggatcg 901 tatgccctcc gtgtgcaagg cgaaccggca aaaggcgaaa tgcttgttgg 951 cacggccgtg tacaacggcg aagtgctgca tttccatatg gaaaacggcc1001 gtccgtaccc gtccggaggc aggtttgccg caaaagtcga tttcggcagc1051 aaatctgtgg acggcattat cgacagcggc gatgatttgc atatgggtac1101 gcaaaaattc aaagccgcca tcgatggaaa cggctttaag gggacttgga1151 cggaaaatgg cggcggggat gtttccggaa ggttttacgg cccggccggc1201 gaggaagtgg cgggaaaata cagctatcgc ccgacagatg ctgaaaaggg1251 cggattcggc gtgtttgccg gcaaaaaaga tcgggattga这对应于氨基酸序列<SEQ ID 71;ORF 287.ng>:g287.pep  1 MFKRSVIAMA CIFPLSACGG GGGGSPDVKS ADTPSKPAAP VVAENAGEGV 51 LPKEKKDEEA AGGAPQADTQ DATAGEGSQD MAAVSAENTG NGGAATTDNP101 KNEDAGAQND MPQNAAESAN QTGNNQPAGS SDSAPASNPA PANGGSDFGR151 TNVGNSVVID GPSQNITLTH CKGDSCNGDN LLDEEAPSKS EFEKLSDEEK201 IKRYKKDEQR ENFVGLVADR VKKDGTNKYI IFYTDKPPTR SARSRRSLPA251 EIPLIPVNQA DTLIVDGEAV SLTGHSGNIF APEGNYRYLT YGAEKLPGGS301 YALRVQGEPA KGEMLVGTAV YNGEVLHFHM ENGRPYPSGG RFAAKVDFGS351 KSVDGIIDSG DDLHMGTQKF KAAIDGNGFK GTWTENGGGD VSGRFYGPAG401 EEVAGKYSYR PTDAEKGGFG VFAGKKDRD*m287/g287 ORFs 287和287.ng有70.1%相同性(在499个氨基酸的重迭区)                 10       20      30       40              49m287.pep    MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVSE-----------KETEA          |||||||||||| |||||||||||||||||||| ||||||||:|          |: ||g287       MFKRSVIAMACIFPLSACGGGGGGSPDVKSADTPSKPAAPVVAENAGEGVLPKEKKDEEA                 10      20       30      40       50       60          50      60       70       80      90      100     109m287.pep    KEDAPQAGSQGQGAPSAQGSQDMAAVSEENTGNGGAVTADNPKNEDEVAQNDMPQNAAGT             |||| :|  | :::||||||||| ||||||||:|:|||||||  ||||||||||g287       AGGAPQADTQD--ATAGEGSQDMAAVSAENTGNGGAATTDNPKNEDAGAQNDMPQNAA--                 70        80       90     100      110         110      120      130     140      150      160     169m287.pep    DSSTPNHTPDPNMLAGNMENQATDAGESSQPANQPDMANAADGMQGDDPSAGGQNAGNTAg287       ------------------------------------------------------------         170      180     190      200      210      220     229m287.pep    AQGANQAGNNQAAGSSDPIPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLTHCKGDS           ::|||:|||| |||||  ||||||||||||:|||::::|:|:|||||||||||||||||g287       -ESANQTGNNQPAGSSDSAPASNPAPANGGSDFGRTNVGNSVVIDGPSQNITLTHCKGDS            120      130     140      150      160      170         230      240     250      260      270      280     289m287.pep    CSGNNFLDEEVQLKSEFEKLSDADKISNYKKDGKNDKFVGLVADSVQMKGINQYIIFYKP          |:|:|:||||:  ||||||||| :||: |||| : ::||||||| |:  | |:|||||g287       CNGDNLLDEEAPSKSEFEKLSDEEKIKRYKKDEQRENFVGLVADRVKKDGTNKYIIFYTD            180      190      200     210      220      230

         290      300      310     320      330      340     349m287.pep    KPTSFARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRYLT          ||  :    ||||||||||||:||||||||||||||||||||||||||||||||||||||g287       KPPT-----RSARSRRSLPAEIPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRYLT                240      250      260     270      280      290         350      360     370      380      390      400     409m287.pep    YGAEKLPGGSYALRVQGEPAKGEMLAGAAVYNGEVLHFHTENGRPYPTRGRFAAKVDFGS          |||||||||||||||||||||||||:|:||||||||||| |||||||: |||||||||||g287       YGAEKLPGGSYALRVQGEPAKGEMLVGTAVYNGEVLHFHMENGRPYPSGGRFAAKVDFGS                300      310     320      330      340      350         410      420      430     440      450      460     469m287.pep    KSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGSGDVSGKFYGPAGEEVAGKYSYR          |||||||||||||||||||||||||||||||||||||:|||||:||||||||||||||||g287       KSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGRFYGPAGEEVAGKYSYR                360      370     380      390      400      410         470      480     489m287.pep    PTDAEKGGFGVFAGKKEQDX          ||||||||||||||||::||g287       PTDAEKGGFGVFAGKKDRDX                420      430下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 72>:a287.seq   1 ATGTTTAAAC GCAGTGTGAT TGCAATGGCT TGTATTGTTG CCCTTTCAGC  51 CTGTGGGGGC GGCGGTGGCG GATCGCCCGA TGTTAAGTCG GCGGACACGC 101 TGTCAAAACC TGCCGCCCCT GTTGTTACTG AAGATGTCGG GGAAGAGGTG 151 CTGCCGAAAG AAAAGAAAGA TGAGGAGGCG GTGAGTGGTG CGCCGCAAGC 201 CGATACGCAG GACGCAACCG CCGGAAAAGG CGGTCAAGAT ATGGCGGCAG 251 TTTCGGCAGA AAATACAGGC AATGGCGGTG CGGCAACAAC GGATAATCCC 301 GAAAATAAAG ACGAGGGACC GCAAAATGAT ATGCCGCAAA ATGCCGCCGA 351 TACAGATAGT TCGACACCGA ATCACACCCC TGCACCGAAT ATGCCAACCA 401 GAGATATGGG AAACCAAGCA CCGGATGCCG GGGAATCGGC ACAACCGGCA 451 AACCAACCGG ATATGGCAAA TGCGGCGGAC GGAATGCAGG GGGACGATCC 501 GTCGGCAGGG GAAAATGCCG GCAATACGGC AGATCAAGCT GCAAATCAAG 551 CTGAAAACAA TCAAGTCGGC GGCTCTCAAA ATCCTGCCTC TTCAACCAAT 601 CCTAACGCCA CGAATGGCGG CAGCGATTTT GGAAGGATAA ATGTAGCTAA 651 TGGCATCAAG CTTGACAGCG GTTCGGAAAA TGTAACGTTG ACACATTGTA 701 AAGACAAAGT ATGCGATAGA GATTTCTTAG ATGAAGAAGC ACCACCAAAA 751 TCAGAATTTG AAAAATTAAG TGATGAAGAA AAAATTAATA AATATAAAAA 801 AGACGAGCAA CGAGAGAATT TTGTCGGTTT GGTTGCTGAC AGGGTAGAAA 851 AGAATGGAAC TAACAAATAT GTCATCATTT ATAAAGACAA GTCCGCTTCA 901 TCTTCATCTG CGCGATTCAG GCGTTCTGCA CGGTCGAGGC GGTCGCTTCC 951 GGCCGAGATG CCGCTGATTC CCGTCAATCA GGCGGATACG CTGATTGTCG1001 ATGGGGAAGC GGTCAGCCTG ACGGGGCATT CCGGCAATAT CTTCGCGCCC1051 GAAGGGAATT ACCGGTATCT GACTTACGGG GCGGAAAAAT TGTCCGGCGG1101 ATCGTATGCC CTCAGTGTGC AAGGCGAACC GGCAAAAGGC GAAATGCTTG1151 CGGGCACGGC CGTGTACAAC GGCGAAGTGC TGCATTTCCA TATGGAAAAC1201 GGCCGTCCGT CCCCGTCCGG AGGCAGGTTT GCCGCAAAAG TCGATTTCGG1251 CAGCAAATCT GTGGACGGCA TTATCGACAG CGGCGATGAT TTGCATATGG1301 GTACGCAAAA ATTCAAAGCC GTTATCGATG GAAACGGCTT TAAGGGGACT1351 TGGACGGAAA ATGGCGGCGG GGATGTTTCC GGAAGGTTTT ACGGCCCGGC1401 CGGCGAAGAA GTGGCGGGAA AATACAGCTA TCGCCCGACA GATGCGGAAA1451 AGGGCGGATT CGGCGTGTTT GCCGGCAAAA AAGAGCAGGA TTGA这对应于氨基酸序列<SEQ ID 73;ORF 287.a>:a287.pep  1 MFKRSVIAMA CIVALSACGG GGGGSPDVKS ADTLSKPAAP VVTEDVGEEV 51 LPKEKKDEEA VSGAPQADTQ DATAGKGGQD MAAVSAENTG NGGAATTDNP101 ENKDEGPQND MPQNAADTDS STPNHTPAPN MPTRDMGNQA PDAGESAQPA151 NQPDMANAAD GMQGDDPSAG ENAGNTADQA ANQAENNQVG GSQNPASSTN201 PNATNGGSDF GRINVANGIK LDSGSENVTL THCKDKVCDR DFLDEEAPPK251 SEFEKLSDEE KINKYKKDEQ RENFVGLVAD RVEKNGTNKY VIIYKDKSAS301 SSSARFRRSA RSRRSLPAEM PLIPVNQADT LIVDGEAVSL TGHSGNIFAP351 EGNYRYLTYG AEKLSGGSYA LSVQGEPAKG EMLAGTAVYN GEVLHFHMEN401 GRPSPSGGRF AAKVDFGSKS VDGIIDSGDD LHMGTQKFKA VIDGNGFKGT451 WTENGGGDVS GRFYGPAGEE VAGKYSYRPT DAEKGGFGVF AGKKEQD*

m287/a287 ORFs 287和287.a有77.2%相同性(在501个氨基酸的重迭区)                 10       20      30       40                49m287.pep    MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVSE-----------KETEA          ||||||||||||| ||||||||||||||||||||||||||||:|          |: ||a287       MFKRSVIAMACIVALSACGGGGGGSPDVKSADTLSKPAAPVVTEDVGEEVLPKEKKDEEA                 10      20       30      40       50       60          50      60       70       80      90      100     109m287.pep    KEDAPQAGSQGQGAPSAQGSQDMAAVSEENTGNGGAVTADNPKNEDEVAQNDMPQNAAGT             |||| :|   | :::|:|||||| |||||||||:|:|||:|:||  ||||||||| |a287       VSGAPQADTQ--DATAGKGGQDMAAVSAENTGNGGAATTDNPENKDEGPQNDMPQNAADT                 70        80       90     100      110         110      120      130     140      150      160     169m287.pep    DSSTPNHTPDPNMLAGNMENQATDAGESSQPANQPDMANAADGMQGDDPSAGGQNAGNTA          ||||||||| ||| : :| |||| ||||:||||||||||||||||||||||| :||||||a287       DSSTPNHTPAPNMPTRDMGNQAPDAGESAQPANQPDMANAADGMQGDDPSAG-ENAGNTA         120      130      140      150     160      170         170      180      190     200      210      220     229m287.pep    AQGANQAGNNQAAGSSDPIPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLTHCKGDS           |:|||| |||::||::|  ::||  :||||:|||:::|||: :|: |:|:||||||a287       DQAANQAENNQVGGSQNPASSTNPNATNGGSDFGRINVANGIKLDSGSENVTLTHCKDKV          180      190      200     210      220      230         230      240     250      260      270      280     289m287.pep    CSGNNFLDEEVQLKSEFEKLSDADKISNYKKDGKNDKFVGLVADSVQMKGINQYIIFYKP          |: :|||||:  ||||||||| :||::|||| : ::||||||| |: :| |:|:|:||a287       CD-RDFLDEEAPPKSEFEKLSDEEKINKYKKDEQRENFVGLVADRVEKNGTNKYVIIYKD           240      250     260      270      280      290         290       300      310      320      330      340m287.pep    KP--TSFARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRY          |   :| |||||||||||||||||||||||||||||||||||||||||||||||||||||a287       KSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRY           300      310      320     330      340      350           350     360      370      380     390      400m287.pep    LTYGAEKLPGGSYALRVQGEPAKGEMLAGAAVYNGEVLHFHTENGRPYPTRGRFAAKVDF          ||||||||| |||||| ||||||||||||:|||||||||||| ||||| |: |||||||||a287       LTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGEVLHFHMENGRPSPSGGRFAAKVDF           360      370     380      390      400      410          410      420      430      440      450     460m287.pep    GSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGSGDVSGKFYGPAGEEVAGKYS          ||||||||||||||||||||||||:||||||||||||||:|||||:||||||||||||||a287       GSKSVDGIIDSGDDLHMGTQKFKAVIDGNGFKGTWTENGGGDVSGRFYGPAGEEVAGKYS           420      430     440      450      460      470           470     480     489m287.pep    YRPTDAEKGGFGVFAGKKEQDX          ||||||||||||||||||||||a287       YRPTDAEKGGFGVFAGKKEQDX           480      490406下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 74>:

m406.seq  1 ATGCAAGCAC GGCTGCTGAT ACCTATTCTT TTTTCAGTTT TTATTTTATC 51 CGCCTGCGGG ACACTGACAG GTATTCCATC GCATGGCGGA GGTAAACGCT101 TTGCGGTCGA ACAAGAACTT GTGGCCGCTT CTGCCAGAGC TGCCGTTAAA151 GACATGGATT TACAGGCATT ACACGGACGA AAAGTTGCAT TGTACATTGC201 CACTATGGGC GACCAAGGTT CAGGCAGTTT GACAGGGGGT CGCTACTCCA251 TTGATGCACT GATTCGTGGC GAATACATAA ACAGCCCTGC CGTCCGTACC301 GATTACACCT ATCCACGTTA CGAAACCACC GCTGAAACAA CATCAGGCGG351 TTTGACAGGT TTAACCACTT CTTTATCTAC ACTTAATGCC CCTGCACTCT401 CTCGCACCCA ATCAGACGGT AGCGGAAGTA AAAGCAGTCT GGGCTTAAAT451 ATTGGCGGGA TGGGGGATTA TCGAAATGAA ACCTTGACGA CTAACCCGCG501 CGACACTGCC TTTCTTTCCC ACTTGGTACA GACCGTATTT TTCCTGCGCG551 GCATAGACGT TGTTTCTCCT GCCAATGCCG ATACAGATGT GTTTATTAAC601 ATCGACGTAT TCGGAACGAT ACGCAACAGA ACCGAAATGC ACCTATACAA651 TGCCGAAACA CTGAAAGCCC AAACAAAACT GGAATATTTC GCAGTAGACA701 GAACCAATAA AAAATTGCTC ATCAAACCAA AAACCAATGC GTTTGAAGCT751 GCCTATAAAG AAAATTACGC ATTGTGGATG GGGCCGTATA AAGTAAGCAA801 AGGAATTAAA CCGACGGAAG GATTAATGGT CGATTTCTCC GATATCCGAC851 CATACGGCAA TCATACGGGT AACTCCGCCC CATCCGTAGA GGCTGATAAC901 AGTCATGAGG GGTATGGATA CAGCGATGAA GTAGTGCGAC AACATAGACA951 AGGACAACCT TGA这对应于氨基酸序列<SEQ ID 75;ORF 406>:m406.pep  1 MQARLLIPIL FSVFILSACG TLTGI PSHGG GKRFAVEQEL VAASARAAVK 51 DMDLQALHGR KVALYIATMG DQGSGSLTGG RYSIDALIRG EYINSPAVRT101 DYTYPRYETT AETTSGGLTG LTTSLSTLNA PALSRTQSDG SGSKSSLGLN151 IGGMGDYRNE TLTTNPRDTA FLSHLVQTVF FLRGIDVVSP ANADTDVFIN201 IDVFGTIRNR TEMHLYNAET LKAQTKLEYF AVDRTNKKLLIKPKTNAFEA251 AYKENYALWM GPYKVSKGIK PTEGLMVDFS DIRPYGNHTG NSAPSVEADN301 SHEGYGYSDE VVRQHRQGQP *下列部分DNA序列是在淋病奈瑟球菌中鉴别出的<SEQ ID 76>:g406.seq  1 ATGCGGGCAC GGCTGCTGAT ACCTATTCTT TTTTCAGTTT TTATTTTATC 51 CGCCTGCGGG ACACTGACAG GTATTCCATC GCATGGCGGA GGCAAACGCT101 TCGCGGTCGA ACAAGAACTT GTGGCCGCTT CTGCCAGAGC TGCCGTTAAA151 GACATGGATT TACAGGCATT ACACGGACGA AAAGTTGCAT TGTACATTGC201 AACTATGGGC GACCAAGGTT CAGGCAGTTT GACAGGGGGT CGCTACTCCA251 TTGATGCACT GATTCGCGGC GAATACATAA ACAGCCCTGC CGTCCGCACC301 GATTACACCT ATCCGCGTTA CGAAACCACC GCTGAAACAA CATCAGGCGG351 TTTGACGGGT TTAACCACTT CTTTATCTAC ACTTAATGCC CCTGCACTCT401 CGCGCACCCA ATCAGACGGT AGCGGAAGTA GGAGCAGTCT GGGCTTAAAT451 ATTGGCGGGA TGGGGGATTA TCGAAATGAA ACCTTGACGA CCAACCCGCG501 CGACACTGCC TTTCTTTCCC ACTTGGTGCA GACCGTATTT TTCCTGCGCG551 GCATAGACGT TGTTTCTCCT GCCAATGCCG ATACAGATGT GTTTATTAAC601 ATCGACGTAT TCGGAACGAT ACGCAACAGA ACCGAAATGC ACCTATACAA651 TGCCGAAACA CTGAAAGCCC AAACAAAACT GGAATATTTC GCAGTAGACA701 GAACCAATAA AAAATTGCTC ATCAAACCCA AAACCAATGC GTTTGAAGCT751 GCCTATAAAG AAAATTACGC ATTGTGGATG GGGCCGTATA AAGTAAGCAA801 AGGAATCAAA CCGACGGAAG GATTGATGGT CGATTTCTCC GATATCCAAC851 CATACGGCAA TCATACGGGT AACTCCGCCC CATCCGTAGA GGCTGATAAC901 AGTCATGAGG GGTATGGATA CAGCGATGAA GCAGTGCGAC AACATAGACA951 AGGGCAACCT TGA这对应于氨基酸序列<SEQ ID 77;ORF 406.ng>:g406.pep  1 MRARLLIPIL FSVFILSACG TLTGIPSHGG GKRFAVEQEL VAASARAAVK 51 DMDLQALHGR KVALYIATMG DQGSGSLTGG RYSIDALIRG EYINSPAVRT101 DYTYPRYETT AETTSGGLTG LTTSLSTLNA PALSRTQSDG SGSRSSLGLN151 IGGMGDYRNE TLTTNPRDTA FLSHLVQTVF FLRGIDVVSP ANADTDVFIN201 IDVFGTIRNR TEMHLYNAET LKAQTKLEYF AVDRTNKKLL IKPKTNAFEA251 AYKENYALWM GPYKVSKGIK PTEGLMVDFS DIQPYGNHTG NSAPSVEADN301 SHEGYGYSDE AVRQHRQGQP *

ORF 406.ng与淋病奈瑟球菌中预测的ORF(ORF 406.a)有98.8%相同性(在320个氨基酸的重迭区):g406/m406                 10       20      30       40      50       60g406.pep    MRARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAASARAAVKDMDLQALHGR          |:||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m406       MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAASARAAVKDMDLQALHGR                 10       20      30       40      50       60                 70       80      90      100     110      120g406.pep    KVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDYTYPRYETTAETTSGGLTG          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m406       KVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDYTYPRYETTAETTSGGLTG                 70       80      90      100     110      120                130      140     150      160     170      180g406.pep    LTTSLSTLNAPALSRTQSDGSGSRSSLGLNIGGMGDYRNETLTTNPRDTAFLSHLVQTVF          |||||||||||||||||||||||:||||||||||||||||||||||||||||||||||||m406       LTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNETLTTNPRDTAFLSHLVQTVF                130      140     150      160     170      180                190      200     210      220     230      240g406.pep    FLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNAETLKAQTKLEYFAVDRTNKKLL          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||m406       FLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNAETLKAQTKLEYFAVDRTNKKLL                190      200     210      220     230      240                250      260     270      280     290      300g406.pep    IKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLMVDFSDIQPYGNHTGNSAPSVEADN          ||||||||||||||||||||||||||||||||||||||||||:|||||||||||||||||m406       IKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLMVDFSDIRPYGNHTGNSAPSVEADN                250      260     270      280     290      300                310      320g406.pep    SHEGYGYSDEAVRQHRQGQPX          ||||||||||:||||||||||m406       SHEGYGYSDEVVRQHRQGQPX                310      320下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 78>:a406.seq  1 ATGCAAGCAC GGCTGCTGAT ACCTATTCTT TTTTCAGTTT TTATTTTATC 51 CGCCTGCGGG ACACTGACAG GTATTCCATC GCATGGCGGA GGTAAACGCT101 TCGCGGTCGA ACAAGAACTT GTGGCCGCTT CTGCCAGAGC TGCCGTTAAA151 GACATGGATT TACAGGCATT ACACGGACGA AAAGTTGCAT TGTACATTGC201 AACTATGGGC GACCAAGGTT CAGGCAGTTT GACAGGGGGT CGCTACTCCA251 TTGATGCACT GATTCGTGGC GAATACATAA ACAGCCCTGC CGTCCGTACC301 GATTACACCT ATCCACGTTA CGAAACCACC GCTGAAACAA CATCAGGCGG351 TTTGACAGGT TTAACCACTT CTTTATCTAC ACTTAATGCC CCTGCACTCT401 CGCGCACCCA ATCAGACGGT AGCGGAAGTA AAAGCAGTCT GGGCTTAAAT451 ATTGGCGGGA TGGGGGATTA TCGAAATGAA ACCTTGACGA CTAACCCGCG501 CGACACTGCC TTTCTTTCCC ACTTGGTACA GACCGTATTT TTCCTGCGCG551 GCATAGACGT TGTTTCTCCT GCCAATGCCG ATACGGATGT GTTTATTAAC601 ATCGACGTAT TCGGAACGAT ACGCAACAGA ACCGAAATGC ACCTATACAA651 TGCCGAAACA CTGAAAGCCC AAACAAAACT GGAATATTTC GCAGTAGACA701 GAACCAATAA AAAATTGCTC ATCAAACCAA AAACCAATGC GTTTGAAGCT751 GCCTATAAAG AAAATTACGC ATTGTGGATG GGACCGTATA AAGTAAGCAA801 AGGAATTAAA CCGACAGAAG GATTAATGGT CGATTTCTCC GATATCCAAC851 CATACGGCAA TCATATGGGT AACTCTGCCC CATCCGTAGA GGCTGATAAC901 AGTCATGAGG GGTATGGATA CAGCGATGAA GCAGTGCGAC GACATAGACA951 AGGGCAACCT TGA这对应于氨基酸序列<SEQ ID 79;ORF 406.a>:

a406.pep  1 MQARLLIPIL FSVFILSACG TLTGIPSHGG GKRFAVEQEL VAASARAAVK 51 DMDLQALHGR KVALYIATMG DQGSGSLTGG RYSIDALIRG EYINSPAVRT101 DYTYPRYETT AETTSGGLTG LTTSLSTLNA PALSRTQSDG SGSKSSLGLN151 IGGMGDYRNE TLTTNPRDTA FLSHLVQTVF FLRGIDVVSP ANADTDVFIN201 IDVFGTIRNR TEMHLYNAET LKAQTKLEYF AVDRTNKKLL IKPKTNAFEA251 AYKENYALWM GPYKVSKGIK PTEGLMVDFS DIQPYGNHMG NSAPSVEADN301 SHEGYGYSDE AVRRHRQGQP *m406/a406 ORFs 406和406.a有98.8%相同性(在320个氨基酸的重迭区)                  10       20      30       40      50       60m406.pep     MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAASARAAVKDMDLQALHGR           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||a406        MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAASARAAVKDMDLQALHGR                  10      20      30       40      50       60                  70      80      90      100     110      120m406.pep     KVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDYTYPRYETTAETTSGGLTG           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||a406        KVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDYTYPRYETTAETTSGGLTG                  70      80      90      100     110      120                 130     140     150      160     170      180m406.pep     LTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNETLTTNPRDTAFLSHLVQTVF           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||a406        LTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNETLTTNPRDTAFLSHLVQTVF                 130     140     150      160     170      180                 190     200     210      220     230      240m406.pep    FLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNAETLKAQTKLEYFAVDRTNKKLL           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||a406        FLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNAETLKAQTKLEYFAVDRTNKKLL                 190      200     210      220      230      240                 250      260     270      280      290      300m406.pep    IKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLMVDFSDIRPYGNHTGNSAPSVEADN           |||||||||||||||||||||||||||||||||||||||||:||||| |||||||||||a406        IKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLMVDFSDIQPYGNHMGNSAPSVEADN                 250      260     270      280      290      300                 310      320m406.pep    SHEGYGYSDEVVRQHRQGQPX           ||||||||||:||:|||||||a406        SHEGYGYSDEAVRRHRQGQPX                 310      320下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 80>:

m726.seq  1 ATGACCATCT ATTTCAAAAA CGGCTTTTAC GACGACACAT TGGGCGGCAT 51 CCCCGAAGGC GCGGTTGCCG TCCGCGCCGA AGAATACGCC GCCCTTTTGG101 CAGGACAGGC GCAGGGCGGG CAGATTGCCG CAGATTCCGA CGGCCGCCCC151 GTTTTAACCC CGCCGCGCCC GTCCGATTAC CACGAATGGG ACGGCAAAAA201 ATGGAAAATC AGCAAAGCCG CCGCCGCCGC CCGTTTCGCC AAACAAAAAA251 CCGCCTTGGC ATTCCGCCTC GCGGAAAAGG CGGACGAACT CAAAAACAGC301 CTCTTGGCGG GCTATCCCCA AGTGGAAATC GACAGCTTTT ACAGGCAGGA351 AAAAGAAGCC CTCGCGCGGC AGGCGGACAA CAACGCCCCG ACCCCGATGC401 TGGCGCAAAT CGCCGCCGCA AGGGGCGTGG AATTGGACGT TTTGATTGAA451 AAAGTTATCG AAAAATCCGC CCGCCTGGCT GTTGCCGCCG GCGCGATTAT501 CGGAAAGCGT CAGCAGCTCG AAGACAAATT GAACACCATC GAAACCGCGC551 CCGGATTGGA CGCGCTGGAA AAGGAAATCG AAGAATGGAC GCTAAACATC601 GGCTGA这对应于氨基酸序列<SEQ ID 81;ORF 726>:m726.pep  1 MTIYFKNGFY DDTLGGIPEG AVAVRAEEYA ALLAGQAQGG QIAADSDGRP 51 VLTPPRPSDY HEWDGKKWKI SKAAAAARFA KQKTALAFRL AEKADELKNS101 LLAGYPQVEI DSFYRQEKEA LARQADNNAP TPMLAQIAAA RGVELDVLIE151 KVIEKSARLA VAAGAIIGKR QQLEDKLNTI ETAPGLDALE KEIEEWTLNI201 G*下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 82>:m907-2.seq  1 ATGAGAAAAC CGACCGATAC CCTACCCGTT AATCTGCAAC GCCGCCGCCT 51 GTTGTGTGCC GCCGGTGCGT TGTTGCTCAG TCCTCTGGCG CACGCCGGCG101 CGCAACGTGA GGAAACGCTT GCCGACGATG TGGCTTCCGT GATGAGGAGT151 TCTGTCGGCA GCGTCAATCC GCCGAGGCTG GTGTTTGACA ATCCGAAAGA201 GGGCGAGCGT TGGTTGTCTG CCATGTCGGC ACGTTTGGCA AGGTTCGTCC251 CCGAGGAGGA GGAGCGGCGC AGGCTGCTGG TCAATATCCA GTACGAAAGC301 AGCCGGGCCG GTTTGGATAC GCAGATTGTG TTGGGGCTGA TTGAGGTGGA351 AAGCGCGTTC CGCCAGTATG CAATCAGCGG TGTCGGCGCG CGCGGCCTGA401 TGCAGGTTAT GCCGTTTTGG AAAAACTACA TCGGCAAACC GGCGCACAAC451 CTGTTCGACA TCCGCACCAA CCTGCGTTAC GGCTGTACCA TCCTGCGCCA501 TTACCGGAAT CTTGAAAAAG GCAACATCGT CCGCGCGCTT GCCCGCTTTA551 ACGGCAGCTT GGGCAGCAAT AAATATCCGA ACGCCGTTTT GGGCGCGTGG601 CGCAACCGCT GGCAGTGGCG TTGA这对应于氨基酸序列<SEQ ID 83;ORF 907-2>:m907-2.pep  1 MRKPTDTLPV NLQRRRLLCA AGALLLSPLA HAGAQREETL ADDVASVMRS 51 SVGSVNPPRL VFDNPKEGER WLSAMSARLA RFVPEEEERR RLLVNIQYES101 SRAGLDTQIV LGLIEVESAF RQYAISGVGA RGLMQVMPFW KNYIGKPAHN151 LFDIRTNLRY GCTILRHYRN LEKGNIVRAL ARFNGSLGSN KYPNAVLGAW201 RNRWQWR*下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 84>:

m953.seq  1 ATGAAAAAAA TCATCTTCGC CGCACTCGCA GCCGCCGCCA TCAGTACTGC 51 CTCCGCCGCC ACCTACAAAG TGGACGAATA TCACGCCAAC GCCCGTTTCG101 CCATCGACCA TTTCAACACC AGCACCAACG TCGGCGGTTT TTACGGTCTG151 ACCGGTTCCG TCGAGTTCGA CCAAGCAAAA CGCGACGGTA AAATCGACAT201 CACCATCCCC ATTGCCAACC TGCAAAGCGG TTCGCAACAC TTTACCGACC251 ACCTGAAATC AGCCGACATC TTCGATGCCG CCCAATATCC GGACATCCGC301 TTTGTTTCCA CCAAATTCAA CTTCAACGGC AAAAAACTGG TTTCCGTTGA351 CGGCAACCTG ACCATGCACG GCAAAACCGC CCCCGTCAAA CTCAAAGCCG401 AAAAATTCAA CTGCTACCAA AGCCCGATGG AGAAAACCGA AGTTTGTGGC451 GGCGACTTCA GCACCACCAT CGACCGCACC AAATGGGGCA TGGACTACCT501 CGTTAACGTT GGTATGACCA AAAGCGTCCG CATCGACATC CAAATCGAGG551 CAGCCAAACA ATAA这对应于氨基酸序列<SEQ ID 85;ORF 953>:m953.pep  1 MKKIIFAALA AAAISTASAA TYKVDEYHAN ARFAIDHFNT STNVGGFYGL 51 TGSVEFDQAK RDGKIDITIP IANLQSGSQH FTDHLKSADI FDAAQYPDIR101 FVSTKFNFNG KKLVSVDGNL TMHGKTAPVK LKAEKFNCYQ SPMEKTEVCG151 GDFSTTIDRT KWGMDYLVNV GMTKSVRIDI QIEAAKQ*下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 86>:orf1-1.seq   1 ATGAAAACAA CCGACAAACG GACAACCGAA ACACACCGCA AAGCCCCGAA  51 AACCGGCCGC ATCCGCTTCT CGCCTGCTTA CTTAGCCATA TGCCTGTCGT 101 TCGGCATTCT TCCCCAAGCC TGGGCGGGAC ACACTTATTT CGGCATCAAC 151 TACCAATACT ATCGCGACTT TGCCGAAAAT AAAGGCAAGT TTGCAGTCGG 201 GGCGAAAGAT ATTGAGGTTT ACAACAAAAA AGGGGAGTTG GTCGGCAAAT 251 CAATGACAAA AGCCCCGATG ATTGATTTTT CTGTGGTGTC GCGTAACGGC 301 GTGGCGGCAT TGGTGGGCGA TCAATATATT GTGAGCGTGG CACATAACGG 351 CGGCTATAAC AACGTTGATT TTGGTGCGGA AGGAAGAAAT CCCGATCAAC 401 ATCGTTTTAC TTATAAAATT GTGAAACGGA ATAATTATAA AGCAGGGACT 451 AAAGGCCATC CTTATGGCGG CGATTATCAT ATGCCGCGTT TGCATAAATT 501 TGTCACAGAT GCAGAACCTG TTGAAATGAC CAGTTATATG GATGGGCGGA 551 AATATATCGA TCAAAATAAT TACCCTGACC GTGTTCGTAT TGGGGCAGGC 601 AGGCAATATT GGCGATCTGA TGAAGATGAG CCCAATAACC GCGAAAGTTC 651 ATATCATATT GCAAGTGCGT ATTCTTGGCT CGTTGGTGGC AATACCTTTG 701 CACAAAATGG ATCAGGTGGT GGCACAGTCA ACTTAGGTAG TGAAAAAATT 751 AAACATAGCC CATATGGTTT TTTACCAACA GGAGGCTCAT TTGGCGACAG 801 TGGCTCACCA ATGTTTATCT ATGATGCCCA AAAGCAAAAG TGGTTAATTA 851 ATGGGGTATT GCAAACGGGC AACCCCTATA TAGGAAAAAG CAATGGCTTC 901 CAGCTGGTTC GTAAAGATTG GTTCTATGAT GAAATCTTTG CTGGAGATAC 951 CCATTCAGTA TTCTACGAAC CACGTCAAAA TGGGAAATAC TCTTTTAACG1001 ACGATAATAA TGGCACAGGA AAAATCAATG CCAAACATGA ACACAATTCT1051 CTGCCTAATA GATTAAAAAC ACGAACCGTT CAATTGTTTA ATGTTTCTTT1101 ATCCGAGACA GCAAGAGAAC CTGTTTATCA TGCTGCAGGT GGTGTCAACA1151 GTTATCGACC CAGACTGAAT AATGGAGAAA ATATTTCCTT TATTGACGAA1201 GGAAAAGGCG AATTGATACT TACCAGCAAC ATCAATCAAG GTGCTGGAGG1251 ATTATATTTC CAAGGAGATT TTACGGTCTC GCCTGAAAAT AACGAAACTT1301 GGCAAGGCGC GGGCGTTCAT ATCAGTGAAG ACAGTACCGT TACTTGGAAA1351 GTAAACGGCG TGGCAAACGA CCGCCTGTCC AAAATCGGCA AAGGCACGCT

1401 GCACGTTCAA GCCAAAGGGG AAAACCAAGG CTCGATCAGC GTGGGCGACG1451 GTACAGTCAT TTTGGATCAG CAGGCAGACG ATAAAGGCAA AAAACAAGCC1501 TTTAGTGAAA TCGGCTTGGT CAGCGGCAGG GGTACGGTGC AACTGAATGC1551 CGATAATCAG TTCAACCCCG ACAAACTCTA TTTCGGCTTT CGCGGCGGAC1601 GTTTGGATTT AAACGGGCAT TCGCTTTCGT TCCACCGTAT TCAAAATACC1651 GATGAAGGGG CGATGATTGT CAACCACAAT CAAGACAAAG AATCCACCGT1701 TACCATTACA GGCAATAAAG ATATTGCTAC AACCGGCAAT AACAACAGCT1751 TGGATAGCAA AAAAGAAATT GCCTACAACG GTTGGTTTGG CGAGAAAGAT1801 ACGACCAAAA CGAACGGGCG GCTCAACCTT GTTTACCAGC CCGCCGCAGA1851 AGACCGCACC CTGCTGCTTT CCGGCGGAAC AAATTTAAAC GGCAACATCA1901 CGCAAACAAA CGGCAAACTG TTTTTCAGCG GCAGACCAAC ACCGCACGCC1951 TACAATCATT TAAACGACCA TTGGTCGCAA AAAGAGGGCA TTCCTCGCGG2001 GGAAATCGTG TGGGACAACG ACTGGATCAA CCGCACATTT AAAGCGGAAA2051 ACTTCCAAAT TAAAGGCGGA CAGGCGGTGG TTTCCCGCAA TGTTGCCAAA2101 GTGAAAGGCG ATTGGCATTT GAGCAATCAC GCCCAAGCAG TTTTTGGTGT2151 CGCACCGCAT CAAAGCCACA CAATCTGTAC ACGTTCGGAC TGGACGGGTC2201 TGACAAATTG TGTCGAAAAA ACCATTACCG ACGATAAAGT GATTGCTTCA2251 TTGACTAAGA CCGACATCAG CGGCAATGTC GATCTTGCCG ATCACGCTCA2301 TTTAAATCTC ACAGGGCTTG CCACACTCAA CGGCAATCTT AGTGCAAATG2351 GCGATACACG TTATACAGTC AGCCACAACG CCACCCAAAA CGGCAACCTT2401 AGCCTCGTGG GCAATGCCCA AGCAACATTT AATCAAGCCA CATTAAACGG2451 CAACACATCG GCTTCGGGCA ATGCTTCATT TAATCTAAGC GACCACGCCG2501 TACAAAACGG CAGTCTGACG CTTTCCGGCA ACGCTAAGGC AAACGTAAGC2551 CATTCCGCAC TCAACGGTAA TGTCTCCCTA GCCGATAAGG CAGTATTCCA2601 TTTTGAAAGC AGCCGCTTTA CCGGACAAAT CAGCGGCGGC AAGGATACGG2651 CATTACACTT AAAAGACAGC GAATGGACGC TGCCGTCAGG CACGGAATTA2701 GGCAATTTAA ACCTTGACAA CGCCACCATT ACACTCAATT CCGCCTATCG2751 CCACGATGCG GCAGGGGCGC AAACCGGCAG TGCGACAGAT GCGCCGCGCC2801 GCCGTTCGCG CCGTTCGCGC CGTTCCCTAT TATCCGTTAC ACCGCCAACT2851 TCGGTAGAAT CCCGTTTCAA CACGCTGACG GTAAACGGCA AATTGAACGG2901 TCAGGGAACA TTCCGCTTTA TGTCGGAACT CTTCGGCTAC CGCAGCGACA2951 AATTGAAGCT GGCGGAAAGT TCCGAAGGCA CTTACACCTT GGCGGTCAAC3001 AATACCGGCA ACGAACCTGC AAGCCTCGAA CAATTGACGG TAGTGGAAGG3051 AAAAGACAAC AAACCGCTGT CCGAAAACCT TAATTTCACC CTGCAAAACG3101 AACACGTCGA TGCCGGCGCG TGGCGTTACC AACTCATCCG CAAAGACGGC3151 GAGTTCCGCC TGCATAATCC GGTCAAAGAA CAAGAGCTTT CCGACAAACT3201 CGGCAAGGCA GAAGCCAAAA AACAGGCGGA AAAAGACAAC GCGCAAAGCC3251 TTGACGCGCT GATTGCGGCC GGGCGCGATG CCGTCGAAAA GACAGAAAGC3301 GTTGCCGAAC CGGCCCGGCA GGCAGGCGGG GAAAATGTCG GCATTATGCA3351 GGCGGAGGAA GAGAAAAAAC GGGTGCAGGC GGATAAAGAC ACCGCCTTGG3401 CGAAACAGCG CGAAGCGGAA ACCCGGCCGG CTACCACCGC CTTCCCCCGC3451 GCCCGCCGCG CCCGCCGGGA TTTGCCGCAA CTGCAACCCC AACCGCAGCC3501 CCAACCGCAG CGCGACCTGA TCAGCCGTTA TGCCAATAGC GGTTTGAGTG3551 AATTTTCCGC CACGCTCAAC AGCGTTTTCG CCGTACAGGA CGAATTAGAC3601 CGCGTATTTG CCGAAGACCG CCGCAACGCC GTTTGGACAA GCGGCATCCG3651 GGACACCAAA CACTACCGTT CGCAAGATTT CCGCGCCTAC CGCCAACAAA3701 CCGACCTGCG CCAAATCGGT ATGCAGAAAA ACCTCGGCAG CGGGCGCGTC

3751 GGCATCCTGT TTTCGCACAA CCGGACCGAA AACACCTTCG ACGACGGCAT3801 CGGCAACTCG GCACGGCTTG CCCACGGCGC CGTTTTCGGG CAATACGGCA3851 TCGACAGGTT CTACATCGGC ATCAGCGCGG GCGCGGGTTT TAGCAGCGGC3901 AGCCTTTCAG ACGGCATCGG AGGCAAAATC CGCCGCCGCG TGCTGCATTA3951 CGGCATTCAG GCACGATACC GCGCCGGTTT CGGCGGATTC GGCATCGAAC4001 CGCACATCGG CGCAACGCGC TATTTCGTCC AAAAAGCGGA TTACCGCTAC4051 GAAAACGTCA ATATCGCCAC CCCCGGCCTT GCATTCAACC GCTACCGCGC4101 GGGCATTAAG GCAGATTATT CATTCAAACC GGCGCAACAC ATTTCCATCA4151 CGCCTTATTT GAGCCTGTCC TATACCGATG CCGCTTCGGG CAAAGTCCGA4201 ACACGCGTCA ATACCGCCGT ATTGGCTCAG GATTTCGGCA AAACCCGCAG4251 TGCGGAATGG GGCGTAAACG CCGAAATCAA AGGTTTCACG CTGTCCCTCC4301 ACGCTGCCGC CGCCAAAGGC CCGCAACTGG AAGCGCAACA CAGCGCGGGC4351 ATCAAATTAG GCTACCGCTG GTAA这对应于氨基酸序列<SEQ ID 87;ORF orf1-1>:orf1-1.pep   1 MKTTDKRTTE THRKAPKTGR IRFSPAYLAI CLSFGILPQA WAGHTYFGIN  51 YQYYRDFAEN KGKFAVGAKD IEVYNKKGEL VGKSMTKAPM IDFSVVSRNG 101 VAALVGDQYI VSVAHNGGYN NVDFGAEGRN PDQHRFTYKI VKRNNYKAGT 151 KGHPYGGDYH MPRLHKFVTD AEPVEMTSYM DGRKYIDQNN YPDRVRIGAG 201 RQYWRSDEDE PNNRESSYHI ASAYSWLVGG NTFAQNGSGG GTVNLGSEKI 251 KHSPYGFLPT GGSFGDSGSP MFIYDAQKQK WLINGVLQTG NPYIGKSNGF 301 QLVRKDWFYD EIFAGDTHSV FYEPRQNGKY SFNDDNNGTG KINAKHEHNS 351 LPNRLKTRTV QLFNVSLSET AREPVYHAAG GVNSYRPRLN NGENISFIDE 401 GKGELILTSN INQGAGGLYF QGDFTVSPEN NETWQGAGVH ISEDSTVTWK 451 VNGVANDRLS KIGKGTLHVQ AKGENQGSIS VGDGTVILDQ QADDKGKKQA 501 FSEIGLVSGR GTVQLNADNQ FNPDKLYFGF RGGRLDLNGH SLSFHRIQNT 551 DEGAMIVNHN QDKESTVTIT GNKDIATTGN NNSLDSKKEI AYNGWFGEKD 601 TTKTNGRLNL VYQPAAEDRT LLLSGGTNLN GNITQTNGKL FFSGRPTPHA 651 YNHLNDHWSQ KEGIPRGEIV WDNDWINRTF KAENFQIKGG QAVVSRNVAK 701 VKGDWHLSNH AQAVFGVAPH QSHTICTRSD WTGLTNCVEK TITDDKVIAS 751 LTKTDISGNV DLADHAHLNL TGLATLNGNL SANGDTRYTV SHNATQNGNL 801 SLVGNAQATF NQATLNGNTS ASGNASFNLS DHAVQNGSLT LSGNAKANVS 851 HSALNGNVSL ADKAVFHFES SRFTGQISGG KDTALHLKDS EWTLPSGTEL 901 GNLNLDNATI TLNSAYRHDA AGAQTGSATD APRRRSRRSR RSLLSVTPPT 951 SVESRFNTLT VNGKLNGQGT FRFMSELFGY RSDKLKLAES SEGTYTLAVN1001 NTGNEPASLE QLTVVEGKDN KPLSENLNFT LQNEHVDAGA WRYQLIRKDG1051 EFRLHNPVKE QELSDKLGKA EAKKQAEKDN AQSLDALIAA GRDAVEKTES1101 VAEPARQAGG ENVGIMQAEE EKKRVQADKD TALAKQREAE TRPATTAFPR1151 ARRARRDLPQ LQPQPQPQPQ RDLISRYANS GLSEFSATLN SVFAVQDELD1201 RVFAEDRRNA VWTSGIRDTK HYRSQDFRAY RQQTDLRQIG MQKNLGSGRV1251 GILFSHNRTE NTFDDGIGNS ARLAHGAVFG QYGIDRFYIG ISAGAGFSSG1301 SLSDGIGGKI RRRVLHYGIQ ARYRAGFGGF GIEPHIGATR YFVQKADYRY1351 ENVNIATPGL AFNRYRAGIK ADYSFKPAQH ISITPYLSLS YTDAASGKVR1401 TRVNTAVLAQ DFGKTRSAEW GVNAEIKGFT LSLHAAAAKG PQLEAQHSAG1451 IKLGYRW*

下列部分DNA序列是在脑膜炎奈瑟球菌中鉴别出的<SEQ ID 88>:orf46-2.seq   1 TTGGGCATTT CCCGCAAAAT ATCCCTTATT CTGTCCATAC TGGCAGTGTG  51 CCTGCCGATG CATGCACACG CCTCAGATTT GGCAAACGAT TCTTTTATCC 101 GGCAGGTTCT CGACCGTCAG CATTTCGAAC CCGACGGGAA ATACCACCTA 151 TTCGGCAGCA GGGGGGAACT TGCCGAGCGC AGCGGCCATA TCGGATTGGG 201 AAAAATACAA AGCCATCAGT TGGGCAACCT GATGATTCAA CAGGCGGCCA 251 TTAAAGGAAA TATCGGCTAC ATTGTCCGCT TTTCCGATCA CGGGCACGAA 301 GTCCATTCCC CCTTCGACAA CCATGCCTCA CATTCCGATT CTGATGAAGC 351 CGGTAGTCCC GTTGACGGAT TTAGCCTTTA CCGCATCCAT TGGGACGGAT 401 ACGAACACCA TCCCGCCGAC GGCTATGACG GGCCACAGGG CGGCGGCTAT 451 CCCGCTCCCA AAGGCGCGAG GGATATATAC AGCTACGACA TAAAAGGCGT 501 TGCCCAAAAT ATCCGCCTCA ACCTGACCGA CAACCGCAGC ACCGGACAAC 551 GGCTTGCCGA CCGTTTCCAC AATGCCGGTA GTATGCTGAC GCAAGGAGTA 601 GGCGACGGAT TCAAACGCGC CACCCGATAC AGCCCCGAGC TGGACAGATC 651 GGGCAATGCC GCCGAAGCCT TCAACGGCAC TGCAGATATC GTTAAAAACA 701 TCATCGGCGC GGCAGGAGAA ATTGTCGGCG CAGGCGATGC CGTGCAGGGC 751 ATAAGCGAAG GCTCAAACAT TGCTGTCATG CACGGCTTGG GTCTGCTTTC 801 CACCGAAAAC AAGATGGCGC GCATCAACGA TTTGGCAGAT ATGGCGCAAC 851 TCAAAGACTA TGCCGCAGCA GCCATCCGCG ATTGGGCAGT CCAAAACCCC 901 AATGCCGCAC AAGGCATAGA AGCCGTCAGC AATATCTTTA TGGCAGCCAT 951 CCCCATCAAA GGGATTGGAG CTGTTCGGGG AAAATACGGC TTGGGCGGCA1001 TCACGGCACA TCCTATCAAG CGGTCGCAGA TGGGCGCGAT CGCATTGCCG1051 AAAGGGAAAT CCGCCGTCAG CGACAATTTT GCCGATGCGG CATACGCCAA1101 ATACCCGTCC CCTTACCATT CCCGAAATAT CCGTTCAAAC TTGGAGCAGC1151 GTTACGGCAA AGAAAACATC ACCTCCTCAA CCGTGCCGCC GTCAAACGGC1201 AAAAATGTCA AACTGGCAGA CCAACGCCAC CCGAAGACAG GCGTACCGTT1251 TGACGGTAAA GGGTTTCCGA ATTTTGAGAA GCACGTGAAA TATGATACGA1301 AGCTCGATAT TCAAGAATTA TCGGGGGGCG GTATACCTAA GGCTAAGCCT1351 GTGTTTGATG CGAAACCGAG ATGGGAGGTT GATAGGAAGC TTAATAAATT1401 GACAACTCGT GAGCAGGTGG AGAAAAATGT TCAGGAAATA AGGAACGGTA1451 ATATAAACAG TAACTTTAGC CAACATGCTC AACTAGAGAG GGAAATTAAT1501 AAACTAAAAT CTGCCGATGA AATTAATTTT GCAGATGGAA TGGGAAAATT1551 TACCGATAGC ATGAATGACA AGGCTTTTAG TAGGCTTGTG AAATCAGTTA1601 AAGAGAATGG CTTCACAAAT CCAGTTGTGG AGTACGTTGA AATAAATGGA1651 AAAGCATATA TCGTAAGAGG AAATAATRGG GTTTTTGCTG CAGAATACCT1701 TGGCAGGATA CATGAATTAA AATTTAAAAA AGTTGACTTT CCTGTTCCTA1751 ATACTAGTTG GAAAAATCCT ACTGATGTCT TGAATGAATC AGGTAATGTT1801 AAGAGACCTC GTTATAGGAG TAAATAA这对应于氨基酸序列<SEQ ID 89;ORF orf46-2>:orf46-2.pep  1 LGISRKISLI LSILAVCLPM HAHASDLAND SFIRQVLDRQ HFEPDGKYHL 51 FGSRGELAER SGHIGLGKIQ SHQLGNLMIQ QAAIKGNIGY IVRFSDHGHE101 VHSPFDNHAS HSDSDEAGSP VDGFSLYRIH WDGYEHHPAD GYDGPQGGGY151 PAPKGARDIY SYDIKGVAQN IRLNLTDNRS TGQRLADRFH NAGSMLTQGV201 GDGFKRATRY SPELDRSGNA AEAFNGTADI VKNIIGAAGE IVGAGDAVQG251 ISEGSNIAVM HGLGLLSTEN KMARINDLAD MAQLKDYAAA AIRDWAVQNP301 NAAQGIEAVS NIFMAAIPIK GIGAVRGKYG LGGITAHPIK RSQMGAIALP351 KGKSAVSDNF ADAAYAKYPS PYHSRNIRSN LEQRYGKENI TSSTVPPSNG401 KNVKLADQRH PKTGVPFDGK GFPNFEKHVK YDTKLDIQEL SGGGIPKAKP451 VFDAKPRWEV DRKLNKLTTR EQVEKNVQEI RNGNINSNFS QHAQLEREIN501 KLKSADEINF ADGMGKFTDS MNDKAFSRLV KSVKENGFTN PVVEYVEING551 KAYIVRGNNR VFAAEYLGRI HELKFKKVDF PVPNTSWKNP TDVLNESGNV601 KRPRYRSK*

使用上述程序,在聚合酶链反应(PCR)试验中采用下列寡核苷酸引物,以克隆所示ORF:用于PCR中的寡核苷酸                            表1

                        实施例2                     ORF 919的表达使用表1中所述的用于ORF 919的引物来定位和克隆ORF 919。预计的基因919被克隆在pET载体中并在大肠杆菌中表达。蛋白表达产物和纯化产物用SDS-PAGE分析。在A)图中,显示了对919-His融合蛋白纯化的分析。用纯化的919-His免疫小鼠,并用血清进行Western印迹分析(B图),FACS(C图),杀菌性测试(D图)和ELISA分析(E图)。符号:M1,分子量标记物;PP,纯化的蛋白;TP,脑膜炎奈瑟球菌总蛋白提取物;OMV,脑膜炎奈瑟球菌外膜小泡(vesicle)制备物。箭头指出了主要的重组蛋白产物的位置(A)和脑膜炎奈瑟球菌的免疫原反应性条带(B)。这些试验证实了919是一种暴露在表面的蛋白,并且是一种有用的免疫原。ORF 919的疏水性图、抗原指数、和两亲区域在图10中提供。使用AMPHI程序来预测推定的T细胞表位(Gao et al 1989,J.Immunol 143:3007;Roberts et al.1996,AIDS Res Human Retroviruses 12:593;Quakyi et al.1992,Scand J Immunol Suppl 11:9)。ORF 919的核酸序列及其编码的氨基酸序列在实施例1中提供。

                         实施例3                      ORF 279的表达使用表1中所述的用于ORF 279的引物来定位和克隆ORF 279。预计的基因279被克隆在pGex载体中并在大肠杆菌中表达。蛋白表达产物和纯化产物用SDS-PAGE分析。在A)图中,显示了对279-GST融合蛋白纯化的分析。用纯化的279-GST免疫小鼠,并用血清进行Western印迹分析(B图),FACS(C图),杀菌性测试(D图)和ELISA分析(E图)。符号:M1,分子量标记物;TP,脑膜炎奈瑟球菌总蛋白提取物;OMV,脑膜炎奈瑟球菌外膜小泡(vesicle)制备物。箭头指出了主要的重组蛋白产物的位置(A)和脑膜炎奈瑟球菌的免疫原反应性条带(B)。这些试验证实了279是一种暴露在表面的蛋白,并且是一种有用的免疫原。ORF 279的疏水性图、抗原指数、和两亲区域在图11中提供。使用AMPHI程序来预测推定的T细胞表位(Gao et al 1989,J.Immunol 143:3007;Roberts et al.1996,AIDS Res Human Retroviruses 12:593;Quakyi et al.1992,Scand JImmunol Suppl 11:9)。ORF 279的核酸序列及其编码的氨基酸序列在实施例1中提供。

                           实施例4                        ORF 576的表达使用表1中所述的用于ORF 576的引物来定位和克隆ORF 576。预计的基因576被克隆在pGex载体中并在大肠杆菌中表达。蛋白纯化产物用SDS-PAGE分析。在A)图中,显示了对576-GST融合蛋白纯化的分析。用纯化的576-GST免疫小鼠,并用血清进行Western印迹分析(B图),FACS(C图),杀菌性测试(D图)和ELISA分析(E图)。符号:M1,分子量标记物;TP,脑膜炎奈瑟球菌总蛋白提取物;OMV,脑膜炎奈瑟球菌外膜小泡(vesicle)制备物。箭头指出了主要的重组蛋白产物的位置(A)和脑膜炎奈瑟球菌的免疫原反应性条带(B)。这些试验证实了576是一种暴露在表面的蛋白,并且是一种有用的免疫原。ORF 576的疏水性图、抗原指数、和两亲区域在图12中提供。使用AMPHI程序来预测推定的T细胞表位(Gao et al 1989,J.Immunol 143:3007;Roberts etal.1996,AIDS Res Human Retroviruses 12:593;Quakyi et al.1992,Scand J Immunol Suppl11:9)。ORF 576的核酸序列及其编码的氨基酸序列在实施例1中提供。

                     实施例5                   ORF 519的表达使用表1中所述的用于ORF 519的引物来定位和克隆ORF 519。预计的基因519被克隆在pET载体中并在大肠杆菌中表达。蛋白纯化产物用SDS-PAGE分析。在A)图中,显示了对519-His融合蛋白纯化的分析。用纯化的519-His免疫小鼠,并用血清进行Western印迹分析(B图),FACS(C图),杀菌性测试(D图)和ELISA分析(E图)。符号:M1,分子量标记物;TP,脑膜炎奈瑟球菌总蛋白提取物;OMV,脑膜炎奈瑟球菌外膜小泡(vesicle)制备物。箭头指出了主要的重组蛋白产物的位置(A)和脑膜炎奈瑟球菌的免疫原反应性条带(B)。这些试验证实了519是一种暴露在表面的蛋白,并且是一种有用的免疫原。ORF 519的疏水性图、抗原指数、和两亲区域在图13中提供。使用AMPHI程序来预测推定的T细胞表位(Gao et al 1989,J.Immunol 143:3007;Roberts et al.1996,AIDS Res Human Retroviruses 12:593;Quakyi et al.1992,Scand J Immunol Suppl 11:9)。ORF 519的核酸序列及其编码的氨基酸序列在实施例1中提供。

                           实施例6                        ORF 121的表达使用表1中所述的用于ORF 121的引物来定位和克隆ORF 121。预计的基因121被克隆在pET载体中并在大肠杆菌中表达。蛋白纯化产物用SDS-PAGE分析。在A)图中,显示了对121-His融合蛋白纯化的分析。用纯化的121-His免疫小鼠,并用血清进行Western印迹分析(B图),FACS(C图),杀菌性测试(D图)和ELISA分析(E图)。结果显示121是一种暴露在表面的蛋白。符号:M1,分子量标记物;TP,脑膜炎奈瑟球菌总蛋白提取物;OMV,脑膜炎奈瑟球菌外膜小泡(vesicle)制备物。箭头指出了主要的重组蛋白产物的位置(A)和脑膜炎奈瑟球菌的免疫原反应性条带(B)。这些试验证实了121是一种暴露在表面的蛋白,并且是一种有用的免疫原。ORF 121的疏水性图、抗原指数、和两亲区域在图14中提供。使用AMPHI程序来预测推定的T细胞表位(Gao et al1989,J.Immunol 143:3007;Roberts et al.1996,AIDS Res Human Retroviruses 12:593;Quakyi et al.1992,Scand J Immunol Suppl 11:9)。ORF 121的核酸序列及其编码的氨基酸序列在实施例1中提供。

                       实施例7                      ORF 128的表达使用表1中所述的用于ORF 128的引物来定位和克隆ORF 128。预计的基因128被克隆在pET载体中并在大肠杆菌中表达。蛋白纯化产物用SDS-PAGE分析。在A)图中,显示了对128-His融合蛋白纯化的分析。用纯化的128-His免疫小鼠,并用血清进行Western印迹分析(B图),FACS(C图),杀菌性测试(D图)和ELISA分析(E图)。结果显示128是暴露在表面的蛋白。符号:M1,分子量标记物;TP,脑膜炎奈瑟球菌总蛋白提取物;OMV,脑膜炎奈瑟球菌外膜小泡(vesicle)制备物。箭头指出了主要的重组蛋白产物的位置(A)和脑膜炎奈瑟球菌的免疫原反应性条带(B)。这些试验证实了128是一种暴露在表面的蛋白,并且是一种有用的免疫原。ORF 128的疏水性图、抗原指数、和两亲区域在图15中提供。使用AMPHI程序来预测推定的T细胞表位(Gao et al 1989,J.Immunol 143:3007;Roberts et al.1996,AIDS Res Human Retroviruses 12:593;Quakyi etal.1992,Scand J Immunol Suppl 11:9)。ORF 128的核酸序列及其编码的氨基酸序列在实施例1中提供。

                        实施例8                      ORF 206的表达使用表1中所述的用于ORF 206的引物来定位和克隆ORF 206。预计的基因206被克隆在pET载体中并在大肠杆菌中表达。蛋白表达产物和纯化产物用SDS-PAGE分析。在A)图中,显示了对206-His融合蛋白纯化的分析。用纯化的206-His免疫小鼠,并用血清进行Western印迹分析(B图)。值得注意的是,在脑膜炎球菌的蛋白质提取物中的免疫反应性条带是38kDa而不是17kDa(图A)。为了获得与该抗体染色的本质有关的信息,我们在大肠杆菌中以没有His-尾但包含预计的前导肽的形式表达ORF 206。对表达这种天然形式206蛋白的大肠杆菌总蛋白提取物的Western印迹分析表明,在38kDa处有一反应性条带,这与脑膜炎球菌中所观察的一样。我们得出结论,在图B)中的38kDa条带是特异性的,而且抗206抗体可能识别多聚体的蛋白复合体。在图C中显示了FACS分析,在图D中显示了杀菌性测试,并在图E中显示了ELISA分析。结果显示296是一种暴露在表面的蛋白。符号:M1,分子量标记物;TP,脑膜炎奈瑟球菌总蛋白提取物;OMV,脑膜炎奈瑟球菌外膜小泡(vesicle)制备物。箭头指出了主要的重组蛋白产物的位置(A)和脑膜炎奈瑟球菌的免疫原反应性条带(B)。这些试验证实了206是一种暴露在表面的蛋白,并且是一种有用的免疫原。ORF 206的疏水性图、抗原指数、和两亲区域在图16中提供。使用AMPHI程序来预测推定的T细胞表位(Gao et al1989,J.Immunol 143:3007;Roberts et al.1996,AIDS Res Human Retroviruses 12:593;Quakyi et al.1992,Scand J Immunol Suppl 11:9)。ORF 206的核酸序列及其编码的氨基酸序列在实施例1中提供。

                        实施例9                     ORF 287的表达使用表1中所述的用于ORF 287的引物来定位和克隆ORF 287。预计的基因287被克隆在pGex载体中并在大肠杆菌中表达。蛋白纯化产物用SDS-PAGE分析。在A)图中,显示了对287-GST融合蛋白纯化的分析。用纯化的287-GST免疫小鼠,并用血清进行FACS(B图),杀菌性测试(C图)和ELISA分析(D图)。结果显示287是暴露在表面的蛋白。符号:M1,分子量标记物。箭头指出了主要的重组蛋白产物的位置(A)。这些试验证实了287是一种暴露在表面的蛋白,并且是一种有用的免疫原。ORF 287的疏水性图、抗原指数、和两亲区域在图17中提供。使用AMPHI程序来预测推定的T细胞表位(Gao et al 1989,J.Immunol 143:3007;Roberts et al.1996,AIDS Res HumanRetroviruses 12:593;Quakyi et al.1992,Scand J Immunol Suppl 11:9)。ORF 287的核酸序列及其编码的氨基酸序列在实施例1中提供。

                     实施例10                     ORF 406的表达使用表1中所述的用于ORF 406的引物来定位和克隆ORF 406。预计的基因406被克隆在pET载体中并在大肠杆菌中表达。蛋白纯化产物用SDS-PAGE分析。在A)图中,显示了对406-His融合蛋白纯化的分析。用纯化的406-His免疫小鼠,并用血清进行Western印迹分析(B图),FACS(C图),杀菌性测试(D图)和ELISA分析(E图)。结果显示406是暴露在表面的蛋白。符号:M1,分子量标记物;TP,脑膜炎奈瑟球菌总蛋白提取物;OMV,脑膜炎奈瑟球菌外膜小泡(vesicle)制备物。箭头指出了主要的重组蛋白产物的位置(A)和脑膜炎奈瑟球菌的免疫原反应性条带(B)。这些试验证实了406是一种暴露在表面的蛋白,并且是一种有用的免疫原。ORF 406的疏水性图、抗原指数、和两亲区域在图13中提供。使用AMPHI程序来预测推定的T细胞表位(Gao et al 1989,J.Immunol 143:3007;Roberts et al.1996,AIDS Res Human Retroviruses 12:593;Quakyi etal.1992,Scand J Immunol Suppl 11:9)。ORF 406的核酸序列及其编码的氨基酸序列在实施例1中提供。

上述实施例用于阐明本发明,而不是用于限制本发明。

                              附录A下列DNA序列是在脑膜炎奈瑟球菌B中鉴别出的<SEQ ID NO:1>:taaaccttatccacatccaaacgcataaccgtaacccattcaccgttatggaaatgtcgcccgacaaccacccagccgaatgattcataaaatatttgcacatcaggcgtataaagatacaagaactttatccccagcgaacgcgctgcgcctatgcagtgggcgaccagcctcctgccaatgccttttccgcgatattcaggtaaaacaaagacatcccccaaccaatattcataccgtggaaaactttccatatcatgccgcttgaccgcagccgaacccaacaggattccggaatcatccacagccgcaaatgccagcggcagttcgtcatccttcaaacacctgccgtaataggcatgaatcttatccacagaagaccacggttcaaatccgtgccactcctcaaacaacgcctgaaccaacctgccgatatgcccggctttcagccgtgtaatgaaaacagtattgtccacaaagagggaattcatcggtcaattccccgacgccttcgttccccctgcgccgtaaaccgcattccaagcatggtccaaacgcactccgatttgcctcaaatcttcagcctgccgggctttttgcgccattgctgcaggaatttccgcttccaaacgggcgatgtctgcctgagccgtctgcaaacgccggcgcgcatcttccaaatccgactgcatcccgatgatttttccgtccagattgttttgcttttgcaataaggcgcggtaaccggattggatgctgagcagattgtcttcagcatcccctgcccatacgcttgtagaaaaaacaaccatcagaaaataaaatatttttttcatttttaacttccatttaaatgctgtctgaagccgtattccgacatcagacggcatcgcccacgcctgtggataacttaagcgcggatgcgtttcaacacttcttctttgccgattaatgccaacacagcatcgacgctgggggttttcgccgtaccgcagacggcaaggcgcaggggcatgccgagtttgcccattttaatgccttcttcgtcgcagaagggtttgaagaggtcgtggatggcttcggcattccagtcttccagcccttcgaggcgttcggcaaagcgcagcatacgggcggcggcttcatcgtcccagtgtttctgcacgtctgcttcggcaggcgtttgtttgacgtagaagtagaagcactcgtcggcaagcgtgttcaagtcttgggggcggtctttgaccagttccaacacatcttccaaagcaggtttttcggtttcatgaatatcgcgcaacgcaaggcggggtttgacgagttcggcgagtttgccgttgggtgtgattttgatgtgttcgccgttgatccagtagagttttttcaagtccatacggcttggagacggggaaacgtctttcaaatcaaaccattcgatgaattgttccattgtgaagaattcatcgtcgccgtgcgcccagcccaagcgtgccagatagttgagcatcgcttcgggcaggatgcccattgcgccgaaatcggtaatggcaacggtatcgccgctgcgtttggagatttttttgccttgttcgttaagaatcatcggcaggtggccgtattcgggcaggttcgcgtcgatggcttttaagatgttgatttgtttcggcgtgttgttcacatggtcgtcgccgcggataacgtgggtaacgcccatgtcgtagtcgtctacgacaacgcagaagttgtaggtcggcgtaccgtcggcgcgggcgataatcaggtcatcgagtgcttcgttggggatggagatttcgcctttgaccaagtctgtccatttggtcacaccgtccaaaggcgttttgaaacggacaacgggttgtacgtcggacgggatttcgggcagggttttacctacttccggacgccagcggcggtcgtaagtcgccgagccttctttttcggctttctcacgcatggcttccagctcttctttgctgcaatagcagtagtaggcatggcctttttctaaaagttcggcaatgacctctttgtagcggtcgaaacggcgagtttggtaaacgacgttgtcggcgttgtcgtaattgagaccgacccatttcatgccgtcgaggatgatgttgacggattcggcggtagaacgcgccaagtcggtgtcttcaatacgtaataggaactcgcctttatgatggcgggcaaacgcccatgaaaacaaggcggtgcgcacgccgccgatgtgcaggtagccggtggggctgggggcgaaacgggttttgacggtcatgatggctccgaaatctttgaaagcgtttattttactggttttaccgtgcttgggcatcaaaaatgccgtctgaaccctgcctgcggataaagtttcagacggcattttccttgttttcaatgcttcggcacgcggaacagtgtatcacgcgccgccgaccgaattccttcgggattgcgtccaaaaaaaagttcaatgaaacagctaattgaaaaaatcccgcccccatttttccaaacggtagagggataacgcatatccctcttgcagcataaagatttttttcttatttcccgcatcaaaccgcgtggtcggcgtggcagacatataaacgcggacacccaaatcctccgccatttccgccgcccgcgccaaatggtagggatcgctgacaatcaccacgctggcaataccgttggcacgcaaaaccggacggatgttgttcaggttttcataagtgttgcgcgaagtgttttcaaacaggatgttgcgcgccggaaccccctgtttgagtgcgtaccgccgcccgacctcggcttcggtcatatagccttttttggtccggcctcccgtaaacacgattttgcctaccctgcggctctgataaagtgcgatggcatggttgatgcgttcgcggaaaacaggagaagggcgtttgtcccacgcggcggcgcccaacaccagcgcggcatccgcccggacatacggcggcaaaacctgcccacccgtccgataaaccgcccaaacggatgaggcaaacaccagcaaaagcggaaaaacactcaaacagaaaccgcccaacaggtaatagcgcaagccgttgcggctgcaaaacagccgtttgttcacaataccgcttcgatattttccagcggtctgccgacagccgccttaccgtttgccaaaacaatcggacgctccaacagggcgggatgatcggcgatggcacgcagcagcgcgtcattgtccaaattggggttgtccaaacccaattccttatacaaatcatctttcacgcgcatcatcccgcgcgccgatgccaagcccaatttgttgaaaatatccttcaattcggacaagtcgggcggcgtatccaaatatttgaccacttcggcagcaatgccgcgttcttccaatagggacaaggcggcacgcgatttgctgcaacgcggattgtggaaaattttgatttcaggcatgacatttccttgcttctcgacaatccccttattatcggcttacacagggttttactcaatatcccgcctacaaccgtaccaaacggtttacaatacccgaatcgacatacaaaggacaaaacgatgaaatacttgaatcttgccgcaatcacccttgccgccacatttgccgcacataccgcctcggcagacgaactggccggatggaaagacaacaccccgcaaagcctgcaatcgctcaaagcccccgtacgcatcgtcaacctttgggcgacttggtgcggcccgtgccgaaaagagatgcctgccatgtccaaatggtacaaagcgcagaaaaaaggcagcgtcgatatggtcggcatcgcgctcgacacatccgacaatatcggcaacttcctcaaacaaactcctgtttcctacccgatttggcgttacaccggggcgaacagccgaaactttatgaaaacctacggaaacactgtcggcgtactgccctttaccgtcgtcgaagcaccgaaatgcggatacaggcagaccattaccggggaggtaaacgaaaaaagcctgaccgacgccgtcaaactcgcccattcaaaatgccgttaaacgccggatgccgtctgaagccgcttcagatggcatttttcttttccacccgcctgccggtgcaaacttatccactatctaaaaacaggcggaatctttataatcggcactgtcttacctattgttcagacggcatatccctgcggacgcaaccgcccgaaacgatatgccgcccttccttacaggacctcctatgatccgtttcgaacaagtttccaaaacctatcccggcggttttgaagccctgaaaaacgtcagcttccaaatcaacaaaggcgaaatgatatttatcgcgggacactccggttcgggcaaatccaccatcctcaaactgatttcgggcattaccaagccgagcaggggcaaaatcctgtttaacgggcaggacctcggcacattgtccgacaaccaaatcggctttatgcgccaacacatcggcatcgtgttccaagaccacaaaatcctctacgaccgcaacgtcctgcaaaacgtcatcctgccgcttcggattatcggctatccgccgcgcaaagccgaagagcgtgcccgcatcgccatcgaaaaagtcggcctgaaaggacgagaattggacgatcccgtaaccctctccggcggtgaacaacaacgcctgtgcatcgcccgcgccgtcgttcaccagcccggcctgctgattgccgacgaaccctccgccaacctcgaccgcgcctacgcgctcgatattatggaattgttcaaaaccttccacgaagcgggaactaccgtcatcgttgccgcacatgacgaaaccctgatggcggactacggacaccgcatcctgcgcctctcgaaaggacgactcgcatgagcatcatccactacctctcgctgcacgtcgaatccgcgcgcaccgcgctcaagcagctcctgcgccaacccttcggcacactgcttaccctcatgatgctcgccgtcgcgatgaccctgccgctgtttatgcatctgggcatccaaagcgggcaaagcgtgttgggcaaactcaacgagtcgccgcaaatcacaatctatatggaaacctccgccgcacaaagcgacagcgataccgtccgcagcctgctggcgcgcgacaaacggctcgacaacatccgcttcatcggcaaagaagacggtctggaagaattacagtccaatcttgaccaaaatctgatttccatgcttgacggcaaccccctgccggatgtctttatcgttacccccgacccggcaaccacgcccgcccaaatgcaggcaatctaccgagacattaccaaactgcctatggtcgaatccgcgtctatggataccgaatgggtgcaaacgctgtaccaaatcaacgagttcatccgcaaaattttgtggtttctttccctgacgctggggatggcgttcgtccttgtcgcacacaacaccatccgcctgcaaatcctcagccgcaaagaagaaatcgaaatcaccaaactcttgggcgcgcccgcgtcgtttatccgccgcccattcctttatcaagccatgtggcagagcatcctttccgccgccgtcagcttggggctttgcggttggctgctctctgccgtgcgcccattggtcgatgccattttcaaaccctacggacttaatatcggctggcggttcttctacgctggcgaactcgggctggtgttcggcttcgtcatcgcgttgggcgtattcggcgcgtggcttgccaccacccagcacctgctcggcttcaaagccaaaaaataaaacaccgtcaaaaatgccgtccgaacccgttttcagacggcatttcaatttgccagtataatggcgcatttt

附录Btccaacaaggaacctaccatgctgacctcggaacaagtaaaagccatgattgaaggcgtggcaaaatgcgaacatatcgaagtagaaggcgacggacaccattttttcgccgtcatcgtttcatcagaatttgaaggcaaggcacgcctcgcgcgccaccgcctgattaaagacggactcaaagcccaactggaaagtaacgaactgcacgcactttccatttcggttgccgccactccggcggaatgggcagccaaagcacaataatcgccacacaaaaatgccgtctgaaaccatttcgtttcagacggcattttttttatatcaaaccgcttacgcgccgcgtttttccaaagcggctacggcaggcagctctttgccttccaagaactcaaggaacgcgccgccgccggtggagatgtagccgatttgttcggtaacgccgaatttggcaatcgccgccagcgtgtcgccgccgcccgcaatcgagaacgctttgctttgggcaatggcttcggcaagggctttcgtaccgcctgcgaattggtcaaactcgaacacgccgaccggcccgttccaaacgaccgtaccggcggctttaagcaaatcggcaagcgcggcagcggatttcggaccgatgtccaaaatcatctcgtcttcggcaacgtcggcaatgtctttcaccacagcttccgcatcggcggcaatgtctttcaccacagcttccgcatcggcggcaaaggctttggcaacgacgacatcggtcggcagcggcacagaaccgccttttgccgccattttcgccataatttttttggattcttccaccaaatcgtgttccgccaaagatttgccgatggctttgccttccgccaacaggaaggtgtttgcgataccgccgccgacgatgagttggtcgactttgtccgccagcgattcgaggatggtcagcttggtggacactttgctgccggcaacgatggcaaccatcgggcgcgcgggctgtttcaaggctttgcccaaagcgtcgagttcgcccgccatcaatacgccggcgcaggcaacgggcgcggcttgggcgacggcttcggtcgaggcttgggcgcggtgggcggttccgaacgcgtcattgacgaacacgtcgcacaaagaagcgtaggctttacccagttccaaatcgtttttcttctcgcctttgttgatgcgcacgttttgcagcatgacgacatcgcccgcgttcagggcgggtttgttttcacgccagtcgttcaatactttcacgtctttgcccaacaggctgcccaagtgcgcggcaacgggggcgacatcgtcttcggggtggaactcgccttcggtcgggcggccgagatgggtcatcacgataacggacgcaccgttgtccacgcagtatttaatggacgcgagcgaggcgcggatacgggtgtcgtcgctgattttgccgtctttgaacggtacgttcatatcggcgcggatgaggacggttttgccctgcacgttttgttcggtcagttttaaaaatgccataatcagtccttttcaatcagtgtttgcgatacggaaacaattgatgccgtctgaaggcttcagacggcatcgcaacccgatcagccggatacgcgctcgattttcgcgccgacgctgccgagttttttttcaatattttcataaccgcgatccaagtggtaaatctgttcgaccacggtttcgcctcgcgccgccaaaccggcgataacgaggctggcggacgcacgcaaatccgtcgccttgacgactgcgccggaaagctgttccacaccctgcacaaatgccgtattgccctcggttgtgatgttcgcccccatccggttcaactcggggacgtgcataaagcggttttcaaaaatcgtttccaccacgcggcagcttccctccgccacggcattcaatgccataaactgcgcctgcatatccgtggggaagccggggtggacgaccgtgcggatgtccaccgccttcggacgctgccgcatatcgatggcgatccaatcgtcgcccgcctcaatcaccgcacctgcctcaaccagtttgtccaacaccacttccatcgttttcggcgcggcattccgcaaaaccaccctgccaccggttatcgccaccgcgcacaggaacgtccccgcctcgatccggtcggggacgacgctgtgttcgcagccttgcagctcgtccaccccttccacaatcattgtggacgtaccgatgccgctgattttcgcgcccattttgaccaggcattccgccaaatcgaccacttcaggctcaatggcgcagttttccaaaaccgtcgtaccttccgccagcgtcgccgccatcagcaggttttccgtgccgccgacggtaacgacatccatcgccacgcgcgtacctttgagtttgcctttggctttgacgtaaccgtgttcgataacaatctcagcacccatcgcttccaagcctttcaaatgctgatcgacggggcgcgaaccgatggcgcagccgcccggcaggctgacttgcgcctcgccgaaacgcgccagcgtcgggcccagcaccaaaatcgaagcgcgcatcgttcggaccaactcgtaaggggcgcaggtattgtttaccgtaccgccgttgatttcaaattcgctgatattgtcggtcaggacgcgcgcgcccatcccctgaagcagcttttgcgtggttttcacatctgccagcatagggacgtttttcaggcgcaacgtacccgatgtcagcaaacccgcgcacatcagcggcaatgccgcgtttttcgcgcccgagaccgttatttccccgttgagcgggccgtttgcggagattttcagtttgtccacgtttgttctttcctggtgggtacttgtatagtgaattaacaaaaatcgggacaaggcggcgaagccgcagacagtacagatagtacagaaccgattcacttggtgcttcagcaccttagagaatcgttctctttgagctaaggcgaggcaataccgtactggtttttgttaatccactataatatttcaattctcgggacaacgcataaagcatcacccgatgaaggttgcagaggcggaattataagggattttcgggaaaaatacggaagccgcaccaaagaatttgacgaaatgccgcgctttccgaacaaggattgtcggaagacaaaaaagccgagttttgaaaactcagcttttttgctttatctggtgggtcgtgagcgattcgaacgctcgaccaacggattaaaagtccgctgctctaccggctgagctaacgacccgataagtttggaattttacagaccggccgaaaccctgtcaagccccttgcgggcggacgggcgttatatccgcttatcggcctgtttttttcgtataaaccaaagaagtcaacaccgatgcacccaatgcgccgaacacgaccgacagcgaaacggaaatcgggatatgcacccaatgcattaccagcattttcacaccgataaaacccaacacgaatgccaatccatatttcaggaagataaagcgttccgccacatccgccagcaggaaatacatcgcccgcaagcccagaattgcgaaaatattggaagtcagcacgataaacggatcggtggtaacggcaaagacggcggggatgctgtccacggcaaacacgacatcgctcaattcaatcatgaccagcaccaaaaacagcggcgtggcgatttttttgccgttttcgacggtaaaaaatttctcgccgtgaaattccgtgccgaccggaacgactttcttgacggtattcagcagcctgctgtttgccaaatcctctttctcatcgccttcgggcttcatcatgtgtataccagtatagagcaggaacgcgccaaacagatacagaatccactcaaactgctgaaccagtgccgcgccgacgaaaatcatgacggtgcgcaataccaatgcgcccaatacgccgtacagcagcacgcggtgctgaaactgtggtgcgactttgaagtagccgaatatcatcaggaacacgaaaatattgtcgactgccaacgatttttccaaaatgtagccggtaaagaattccaatactttttcttttgcgactgccgcgccgtagccgggattgccggcgagttcaaaatacagccagcccgcgaacaggcaggatacggcaacccacaagccgctccatgccaaggcttctttgacgccgactttatggctgccgtttttcttcagcgaaaacatatccaaggcaatcatgaccagcactgccgcaaaaaaaacgccgtaaaacaacggcgacccgatgccgggatattctgtcatggttcaatctcctgatttgaaatgtaattgtgttaccagctgatataaaacatcgcttttgccaaaaagacaatcagcagcatatgggtaaagacgacggcgtgtatgtatttcgaccaaccgaccgtcagtgtggaacgcgccattttgacgacggcgatggcgaagtgcgccaatacgctgaacgccaacaggattttcagcgtcagcatcgtaccgaaggaagtggcaaacggttcgcccaatatagaaagatagcggtttgccgccatcacgatgccgctggcgaacagcagtccgaccacaaacggcatcaccctgacggcgcggtaagacattgccttttccacttcgcgccgcgcctcgcgcgacacccgtcccgtatgcaggacggacaaaaccagcacttcaaaaaacacgccgccgacaaaggcaatagcgcaatacagatgaacgatgtgcgcgacggcataaatactcatacgatgctccaaacggaaaactcggatacggattgtatcactatcgcccccgatatccgcataccgcttcccgcaccgcctcggcgattctcgcgcccgctccgcgatgttgtgcgataaagccgtccacgcgcgcctgcatctgcatcccccccccctcggacgataaggttttttcaacggcttcccgccacgcatccgccgattcgacttgaaccgccgcacccgatgccaaggcgtgtcggcaggcttcggaaaaattgtaggttgaaaagccgaatatcgtcggaacgccgcaggaaagcggttcgatgatgttctgacaacccgaatcgaccagactgccgccgacaaaagcgacatcggcgcacaggtaatacgcatacagctcgcccatactgtcgcctatccacacctgcgtatcaggttcgaccggcaaaccgtcgctgcgccgctgaaccttaaacccgaagcgttttgccgtttcaaataccgtctgaaaatgctcgggatggcgcggcacgacgaccagcagcgcatcgccgcgatattgttgccacgccgccagcagtttttccgcctcgtcttcaccccgataaacgcgcgtgctgccgcacacggcaaccggccggcctccgatgcgtttttcaaactgccccgccagcgttttcatctgttccgacggtatgatgtcgtatttggtattgccgcacacctgcacggatgccgcgcccaatttcgccaaccgcgccgcatccgcctctgtctgcgccagacaccccgtcagcgaagcggcggcaggacggatcaggcggcggactttcagataaccgttcaacgatttttccgacagccgcgcattcgccaaaaacagcggcacacccgcgcgccggcattccctcatcaggttgggccagatttcggtttccatcaaaatgccgaacatcgggcggtgttcgcgcaaaaactgccgtacccacgtttttttgtcatacggaagatagcggcattgcgcatcgggaaacagaacttgcgcggtttcccgccccgtcggggtcatctgcgtcatcagcagcggcgcatcgggaaaacgccgccgcaactcgcgtatcaaggactgggcggcacgcgtttctccgaccgaaacggcgtgtatccaaaccgcgccggtaacgggattcggatacggcttgccgaaacgctcgtcccgatgcgcccgatatgccggggcacttccggagcgtttgtccaaataacgccgtatccatatcggcgcaagcagccacaatacatcataaagccattggaacatctttctatttcctgcaaaacaaatgccgtctgaacggttcagacggcatttcggcaacggaatcaaatatcgtaggttgtcgaagcggtatctccgcccttgcccgtccagttggtatggaaaaactcaccgcgcggtttgtcggtgcgctcgtaagtgtgcgcgccgaagtagtcgcgctgtgcctgcaagaggttggcaggcagacgttcggtcgtgtagccgtccaagaacgtaatcgccgaagccatgcagggcatagggatgccgcattcgaccgccttggcaaccaccttgcgccacgccggcaggcagttttccaaaatatttttgaaatacggatccgcacccaagaacaccaaatcgggattgttttcatacgcgtcgcggatattgcttaagaatgcgctgcgaatgatgcacccctcgcgccacagcagcgcagtgttgccgtagtccaaatcccagccgtagctttcgcccgcttcgcggatca

附录Bgcataaagccttgtgcgtaggaaatgattttagatgcaagcagggcctgtctcaacgcctcgacccattcttgtttgccgccttcgacgggcgtaacggttcgggcgaacagtttgccggtctgcacgcgctgttctttgaacgacgaaacgcagcgggcgaatacggcttcggaaatcagcgtcagcggaatacccaaatccaaagcattgatgcccgtccatttgcctgtacctttttgccctgccgtatcgaggattttctcgaccagcggttcgccgccttcgtccttatagcccaaaattgccgctgtgatttcaatcagataagaatccagctcggttttgttccactcggcaaacacgcggtacatttcgtcgtaagacagccccaagccgtctttcatgaactggtacgcttcgcaaatcaactgcatatcgccatattcgatgccgttatgcaccattttgacaaaatgccccgcaccgtctttgccgacccagtcgcaacacggttcgccctgcgacgttttggcggcaatcgcctgaaaaatcggcttgaccgcatcccaagcgcgcttatccccgcccggcataatggacggcccgcgccgcgccccttcttccccgccggacacgcccgcgccgacaaacaaaatccctttttcagcaaggtaatgtgtccgccgtgtcgtgtcggggtaattggcattgccgccgtcgataaggatgtcgccttcttccaacagcggaagcagttgttcgataaattcgtcaaccaccgaaccggcacgaaccatcatcataatttttcgcggtttttccagcttatcgaccaaatcttgcaaagaatacgcgccgataatattagttccttttgccgcgccgtttaaaaattcgtccaccttggcagtcgtgcggttgtaggcaaccaccttaaatccgcaatcgttcatattcaaaatcaggttttgccccataaccgccaaaccgattacaccaatatcgccgttcattgcaggaagctccgttatagatttaatttatcgaccgcaactctacccgatttacacttgtttaacaatccttaactttttaattttttgaaaagatgcctttacgctttgctgtaccgttttgctgaagggttataaataaaatataaaatttaaataataaaacgatgattatattgataggagaaattttctgtgggtaacttttttttattttaaaaatcatcaggatttcttttttttagggtgtcggtaaggcggattcccttttgtgcatacctgtggattgtttttcatgaagaatagtttttgtggacagtttgcttgttgtgcaaatggcatcctacttttctttaccgaatggctgccgatgtctttaagaaccggaatactgtggaggtttgagaggaaagtgtgtttggaacttgtggaaatggtcaggtgtcggcacgaatgtcttatttctgcatatcggcagagtgcgcatccgaatttgtgtataagtggtggaaaaaatgagatttgcgggtaaatctcacaatatttcagtcagataactttggattgcttgtgtataagtaaactttcggatggggatacgtaacggaaacctgtaccgcgtcattcccacgaacctacattccgtcattcccacgaaagtgggaatgatgaaattttgagttttaggaatttatcgggagcaacagaaaccgctccgccgtcattcccgcgcaggcgggaatctagaacgtaaaatctaaagaaaccgtgttgtaacggcagaccgatgccgtcattcccgcgcaggcgggaatctagaccattggacagcggcaatattcaaagattatctgaaagtccgagattctggattcccactttcgtgggaatgacgggatttgagattgcggcatttatcggaaaaaacagaaaccgctccgccgtcattcccgcgcaggcgggaatccagaccttagaacaacagcaatattcaaaggttatctgaaagtccgagattctggattcccactttcgtgggaatgacgggattttaggtttctgattttggttttctgtttttgtgggaatgatgaaattttgagttttaggaatttaccggaaaaaacagaaaccgctccgccgtcattcccgcgcaggcgggaatccagaccttagaataacagcaatattcaaagattatctgaaagtccgggattctagattcccactttcgtgggaatgacggcatcagtctgccgtttacagcacggtttctttagattttacgttctagattcccgcctgcgcgggaatgacgaatccatccatacgaaaacctgcaccacgtcattcccacgaacctacatcccgtcattcccacaaaaacagaaacctcaaatcccgtcattcccgcgcaggcgggaatctagacttgtcggtgcggacgcttatcggataaaacggtttcttgagattccgcgtcctggattcccactttcgcgggaatgacgaattttaggtttctgttttggttttttgtccttgtaggaatgatgaaaatttaagttttaggaatttaccggaaaaaatagaaagcgttatccacaagttctgatgttcagctcgtgaaatgcgtcgggcaaatcatcgctgtcggcaaattccacccggtcgtaagccgtttcgtctgccaaaaccgcgcgcaagagtgcgttgttgatggcgtgtcccgatttgtagccttcaaatgcgccgacaatcggatgtccgacgatatacaaatcaccgatggcatcaaggattttgtggcgcacaaactcatcgggatagcgcaagccttcaggattcaggacatccgtgtcgtcaatcacgatggcgttgttcaaattgccgcccaaacccagattgtgggcgcgcatcatttccacttcgtgcataaagccgaaagtgcgcgcgcgcgcgatttcgtcgatgtaggatttgccggcgaaatcgatttcaaaagtgggcgagctgcggttgaaaaccggatggtcgaattcgatggtcagcgttaccttaaagccgtcatacggcgtaaagcgcacccatttgcccgcttctttgatttcgacaggcttgaggattttcaaaaaacgcttttgcgccttttgatcgaccacgcccgcatcttgcaaaaggtaaataaacggcaggctggagccgtccataatcgggatttcgggcgcgttcagctcaatcagcgcattgtcgatgccgtaggcggacagcgcggacataatgtgttcgatcgtgccgacgcgcacgcctttgtcggtaacgatggtggaggaaaggcgggtatcgttgatcaaataaggggtcagcttgatttgttcgcccatctcgccgtccaaatcggtacggcggaaggaaatcccgctgttttcaggcgcggggtgcagggtcagcgcgacgcgttcgcccgaatgcagcccgacgccggtaacgctgatggatttcgccaaagttctttgcagcataaaccgcttccttatcaagggggtaagttttggaataatacgataaaaccggaaaaacaggctatgtttttccatagtatttgccaatgtatccgttttcaatacgtaagccgcataaaaatgtatagtggattaacaaaaatcaagacaaggcgacgaacccacccccctcctgaaaaacgcaaaaaatgccgtccgaaaacctttcggacggcattttcgcgtaaaccgtcattcccacaaggacaaaaaaccaaaacagaaaaccaaaaacagcaacctaaaattcgtcattcccgcgcaggcgggaatttggaatttcaatgcctcaagaatttatcggaaaaaaccaaaacccttccgccgtcattcccacgaaagtgggaatctagaaatgaaaagcagcaggcatttatcggaaatgaccgaaactgaacggactggattcccgcttttgcgggaatgacggcgacagggttgctgttatagtggatgaacaaaaaccagtacggcgttgcctcggcttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttccgtactatttgtactgtctgcggcttcgtcgccttgtcctgatttttgttaatccactatatctagccgaattactttattttttgatacgtaaccggccggttgccgtcattcccgcgcaggcgggaatctagacattcaatgctaaggcaatttatcgggaatgactgaaactcaaaaagctggattcccactttcgtgggaatgacgcggtgcaggtttccgtacggatagcttcgtcattcccgagtaggcgggaatctagtccgcttgttcggtaaatgagagggcggattgcgcgcctgtcagataaaccacgtgtttaaacgggcggcaatgaggtacgcgcagagccttgaagcgcaatcgatatattattttcagccaaaacggacgcccccgcttgccttgcaaacctttaaaaaggaagccacccggattaatccgagtggccgtggaaaatcacttaccgcttgatttatttaaaatttatggtataatttaccttagctggcatcacttgcgtcgcggcaggttgacggcaggtgcttggtgtcaatcttcttaccgttggcggcggcggcggcggtaacgtcgtcgttggcggctttggctttgtcgcgcgtaaccggctgtccgcagaaccattttaccgaaccgttttgacgcttggcccacagggagagttttttgcctttgatttcgttgtttacgttgcttgaagccatttgggcggtaacgacgccgtttttgacttcaacgcttttaacatatttgcctttgatttcagaggaggttgccacgccggcagaagtgttgttgccgggccattcgccgtgattcaggtaatactcggtaacggctgatttttgaccttcggccaaaagaatggcttcggaaacttgtgcgcgggctgtgtagtcttgataagcaggaagggcgactgccgccaaaatgccgacgatggcaatcacaatcatcagctcgataagggtaaaacctttttgaagggtgttcataaaattactcctaattggaaaggaaatgcctcaagcttacgccatcggcattatgcaatgtatttgaccatcggtattttgttgcgatacctgtgtattataaagcaagattggtaccaagtttgtattttgaggtgaaaatttatgcgtttatctctatgtaattgtttttattttacattttctttcgtttggcgtggtttgagtaattagggggttgccgttttttgtcagcagtgttgaaaattgtcagttttagtgccgattttcggcacttttttattggcgtggggtatctctattggcatggggcatcgggtgtgttgattgggtcggaatttgagatttttgaatttgcgcggtagcatagggtgggttgggtgggaaattttaaatttaatttttaaaaatttccgttttcttggaaagtgattgaaatcggcgcgtggtgttcctgtgcaaccggcagttgaatcatcgcggcaggtttccgtgcggatggcttcgtcattcccgcgcaggcgggaatccagccttgttggtacggaaacttatcgggaaaacggtttcttgagattttacgttctggattcccactttcgcgggaatgacgcggtgcaggtttccgtatggatagcttcgtcattcccgcgcaggcgggaatccaggtctgtcggcacggaaacttatcgggtaaaaaggtttcttgagatttttcgtcctggattcccactttcgtgggaatgacgggatgtaggttcgtgggaatgacggtttaggtatttttatagaaagccgtaggtggtgtttctatgcaaacgacagatgaatcatcgcggcaggttgacggcaggtgcttggtgtcgattttgtcggtgccggtggcggcggcggtaacggcgtcgtctttggcgttgtcggcgcgcgtaaccggcagtccgcagaaccattttaccgaaccggcttgacgcttggcccacagggagagttttttgcctttgatttcgttgtttacgttgcttgaagccatttgggcggtaacgacgccgtttttgacttcaacgcttttaacatatttgcctttgatgtcggcggaggttgccacgccggcagaactgttgttgccgggccattcgccgtgattcaggtaatactctgtgacggctgatttttgaccttcagccaaaagaatggcttcgtcattcccgcgcaggcgggaatctaggtctgtcggcacggaaacttatcgggaaaacagtttcttgagattttgcgttctggattcccgctttcgcgggaatgacgggattaaagtttcaaaatttattctaaataactgaaattcaacgaactagattcccactttcgtgggaatgacgaattttaggttgctgtttttgtgggaatgatgaaattttaagttttaggaatttatcgaaaaaacagaaaccgctccgccgtcattcccgcgcaggcgggaatccagcctcgtcggtacggaaacttatcgggtaaaaaggtttctc

附录Btagtttggtgtcgattttcttgtcgatgctgttgacggcaggtgcttggtgtcgatctgcttgccgttggcggcggtgtcggctttgacggcgtcggcgctggcgttgtcgcgcttaaccggctgtccgtagaaccattttaccgaaccgtcttgacgcttggcccacagggagagttttttgccttggatttctttgtttacgccgcttgaaagcattgtggcggtaacgacgccgtttttgacttcaactttctcaacatatttgcctttgatgttggcggaggttgccacgccggcagaactgttgttgccgggccattcgccgtgattcaggtaatactcggtgacggctgatttttgaccttcagccaaaagaatggcttcgtcattcccgcgcaggcgggaatctagaccttagaacaacagcaatattcaaagattatctgaaagtccgggattctagattcccactttcgtgggaatgacgaattttaggttgctgtttttggttttctgtttttgagggaatgatgaaattttaagttttaggaatttatcagaaaaaacagaaaccgctccgccgtcattcccgcgcaggcgggaatccaggtctgtcggtacggaaacttatcgggtaaaacggtttctctagtttggtgtcgattttcttgtcggtgctgttgacggcaggtgcttggtgttgatgttggcggtgcccttgccggtggcggcggtgacggcgtcgtctttggctttgtcgcgcgtaaccggctgtccgcagaaccattttaccgaaccgttttgacgcttggcccacagggagagttttttgcctttgatttcgttgtttacgttgcttgaagccatttgggcggtaacgacgccgtttttgacttcaacgcttttaacatatttgcctttgatttcagaggaggttgccacgccggcagaactgttgtcgccgggccattcgccgtgattcaggtaatactcggtaacggctgatttttgaccttcgaccaaaaggatagcttcgtcattcccgcgcaggcgggaatccagccttgtcggtacggaaacttatcgggtaaaacggtttctttagattttgcgttctggattcccactttcgtgggaatgacgggattaaagtttcaaaatttattctaaataactgaaactcaacgaactagattcccgcttttgcgggaatgacgaattttaggtttctgttttgggttttctgtttttgagggaatgatgaaattttaggtttctgtttttggttttctgtccttgtgggaatgatgaaattttaagttttaggaatttatcggaaaaaacagaaaccgctccgccgtcattcccgcgcaggcgggaatccagcctcgtcggtgcggaaacttatcgggaaaacggtttctttagattttacgttctggattcctactttcgtgggaaagacgaattttaggtttctgtttttggttttctgtccttgtgggaatgatgaaaatttaagttttaggaatttatcggaaaaaacagaaaccgctctgccgtcattcccgcaaaagcgggaatccagcctcgtcggtgcggaaacttatcgggtaaaaaggtttctttagtttggtgtcgattttgtcggtgccggtggcggcggcaacgtcgtctttggcgttgtcggcgcgcgtaaccggctgtccgcagaaccattttaccgaaccggcttgacgcttggcccacagggagagttttttgcctttgatttcgttgtttacgccggttgaaagcattgtggcggtaacgacgccgtttttgacttcaacttccttaacatatttgcctttgattgttgaagaagatgccacgccggcggcatcattaaatcccgtcattcccactttcgtgggaatgacgggattaaagtttcaaaatttattctaaataactgaaactcaacgaactagattcccgcttttgcgggaatgacgaattttaggttgctgtttttggttttctgtccttgcgggaatgatgaaattttaagttttaggaatttatcgaaaaaacagaaaccgctccgccgtcattcccgcgcaggcgggaatccagcctcgtcggtgcggaaacttatcgggaaaacggtttcttgagattttgcgttctggattcccgctttcgtgggaatgacggtttaggtatttttatagaaagccgtaggtggtgtttctatgcaaacgacagatgaagcgtcgcggcaggttgacggcaggtgcttggtgttgatgttgtcggcggtcttggcggcggcggcgacggtgtcggctttggcgtcggtgcgcgtaaccggctgtccgcagaaccattttaccgaaccgtcttgacgcttggcccacagggagagttttttgccttggatttctttgtttacgccgcttgaaagcattgtggcggtaatgacgccgtttgcgactgtaacttccttaacatatttgcctttgattgttgaagaagatgccacgccggcagaagtgttgttgccgggccattcgccgtgattcaggtaatactctgtgacggctgatttttgaccttcggccaaaaggatagcttcgtcattcccgcgcaggcgggaatccaggtctgtcggtacggaaacttatcgggtaaaacggtttctttagattttgcgttctggattcccactttcgcgggaatgacgggattaaagtttcaaaatttattctaaataactgaaaccaacgaactagattcccacttttgcgggaatgacgaagtttttctgccatttgccgtgattcgggcaatactcggtaacggctgattttttgaaagtgtttgaaatcggcgcgtggtgtttctatgcaaccggtagatgaatcatcgcggcaggttgacggcaggtgcttggtgttgattttgtcgtcggtcttgccgttggcggcggcgacgtcggtggcggtggcggtggcggtgtcgttgcgcgtaaccggctgtccgcagaaccatttgaccgaaccgttttgacgcttggcccacagggagagttttttgcctttgatttctttgtttacgccgcttgaaagcattgtggcggtaacgacgccgtttttgacttcaactttctcaacatatttgcctttgatgtcggaggaggatgccacgccggcggcatcattaaatcccgtcattcccgcaaaagcgggaatctagaactcaggaccggagaaacctttttacccgataagtttccgtgccgacagacctagattcccgcctgcgtgggaatgatgggattaaagtttcaaaatttattctaaataactgaaactcaacgaactagattcccgcttttgcgggaatgacgaattttaggtttctgtttgtgggtttctgttcttgtgggaatgatgaaattttaagttttaggaatttatcggaaaaaacagaaaccgctccgccgtcattcccgcgcaggcgggaatccagccttgtcggtacggaaacttatcgggtaaaaaggtttctctagtttggtgtcgattttcttgtcggtgctgttgacggcaggtgcttggtgttgattttgtcggtgtcgggtgtggcggcggtgacttcgtcggtgccggctttggcgttggcggcgttgcgcgtaaccggctgtccgcagaaccattttaccgaaccgtcttgacgcttggcccacagggagagttttttgccttggatttctttgtttacgccgcttgaaagcattgtggcggtaatgacgccgtttgcgactgtaacttccttaacatatttgcctttgattgttgaagaagatgccacgccggcagaagtgttgtttttcggccattcgccgtgattcgggtaatactcgggtgtttttgtgcaaacggcagatgctgcgtcgcggcaggttgacggcaggtgcttggtgttggttttcttgttgccggtgttgtcggcggcgacggtgtcgtcggtgccggcgcgcgtaaccggctgtccgcagaaccattttaccgaaccgttttgacgcttggcccacagggagagttttttgccttggatttctttgtttacgccgcttgaaagcattgtggcggtaacgacgccgtttgcgactgtaacttccttaacatattttcctttgattttagaggaggatgccacgccggcggcatcattaaatcccgtcattcccacgaaagtgggaatctagaactcaggaccggagaaacctttttacccgataagtttccgtgccgacagacctggattcccgcctgcgcgggaatgacgaagtttttcggccattcgccgtgattcgggcaatactcgggtgttttgtgcaaacggcagatgctgcgtcgcggcaggttgacggcaggtgcttggtgtcaatcttcttaccgttggcggcggcggcggcggtaacgtcgtcgttggcggctttggcgttgtcgcgctcaaccggctgtccgcagaaccattttaccgaaccggcttgacgcttggcccacagggagagttttctgcctttgatttctttgtttacgccgcttgaagccattatgtcagacggtattgcccgggcagctttattcgtacactttcagcagctcgacttcaaatatcaaagtggcgtgcgggggaatcacgccgcccgcgccgtgtgcgccgtagcccatttccgaagggatggtcagcttgcgtttgccgccttccttcatgccgccgaagccttcgtcccagcctttgatgacttgtccgacaccgagcgtgatggtcagcggctggcggcggtcgaggctggagtcgaatttggttccgttttccagccaacctgtgtaatgcacggtaatctctttgcctttaactgcttcttttccgaagccttcttgcaagtcttcaataatcaggccgcccatatttgtcctttcgttgcttgttggtcaaaacggcaagggtaacataccgtccgtcgaagtcaaatgccgctcaaacgtcagctgcatcggtgcagctgaaacggctgtgtttgtttgactgttttatttttttcgtaaaggttccatgctttttcatggaaatagaaaacgacggtgttgattaggggttcgaccagcgcaactgctcccgatacgcctatactgcccgtcagtacataggttacactgaaggcgacgctgaaatgcagtgcggcaaaagtcagggttttaagcatcatcctctcccggattggacattgacggagagatgataaagattatcataaggctgcgcggtttaaatttgctatttgttgttagtgtagataaatcgttttttaaataaggataggaattatgaatcataaaaagatcgttgttttggatgcggatactttgcccggccgggtttttcattttgattttccgcacgagcttgcggtttacggtacgacaggtgcggatgaaacggcagaacgggtgcgcgatgcacatattgtcattactaacaaagtgatgatttctgccgatattattgcggctaatccgcagttggagctgattgccgtcagtgcgaccggcgtgaacaatgtcgatattggggcggcgaaggcggccggtgttgcggtatgcaatgtccgcgcatacggaaacgaatcggttgcggaacacgcctttatgctgatgattgcgttaatgcggaatttgcctgcctatcagcgtgatgttgcggcaggattgtgggaaaagtcgccgtttttctgccattacggcgcgccgattcgggatttgaacggcaaaacgctggcggttttcggacgcggcaatatcggacggacgcttgccggatacgcgcaggcattcggtatgggggtggtgtttgccgaacacaaacacgcgtccgctgtgcgtgaaggctatgtttcctttgaagatgcggtacgggctgctgatgtgttgtcgctgcactgtccgctaaacgcccaaactgaaaatatgataggcgaaaacgaattgcggcagatgaagcctggcgcggttttaatcaattgtgggcgcggcgggctggtggatgaaaacgcgctgcttgccgcactcaaatacgggcagatcggtggggcaggtgtcgatgttttgacgaatgagccgcccaaaaacggcaatcccttgctgaatgcacgattacccaatctgattgttacgccgcataccgcgtgggcaagtcgtgaggctttggacaggctgtttgatatattgttggcgaacattcacgcctttgtgaaaggagaggcgcaaaaccgcgtggtttgaacctgtcgggattgcggaaaaaaatgccgtctgaacgcctcaagggttcagacggcatttcttgagattcccgtttaaccgactttgtcgcccggctgcgcgcctgtatccacatccaagagcttcagtttcccgtctgccgtggcggcactcaaaatcatgccttcagatacaccgaattttgccattttgcgcggggcgaagttggcgacggcgatgaccatgcggccgttcaattcggcagggttcgggtaagacgcggcgatgccggagaagatgatgcgtttttcaaaaccgaaatcgaggtcgaatttcaaaagttt

附录Bggtgctgccttcgacagcttcgcagttcaatactttggcaacgcgcatgtcgattttcataaagtcgtcgaaactcgcctgttcggcgactttttcgtatttgccctcttcggcggcaggtgcggctgcggcggcgatgctttgtttgttggcttcgattaaatcgtccacttgtttttgctccactcgttgcattaaatgttcgtatttgttgatggcgtgtttgcccaaggtatcgcgtgtatttgcccaagtgatggcttccaaattcaggaatttggcggcgtttgcggcggtttgcggcaagacgggggcgaggtaggcggtcaacatggtgaaggcgttgatgagttcgctgcatacttcgtgcaggcgttcgtcttggccttcttgtttggcgagttcccacggcttgttggcatcaacgtattcgttgacaatgtctgccaaggccatgatgtcgcgcagggctttggcgtattcgcggctttcgtagcattcggcaatggcttcgctttgcgcagtcagttttgccagcaattcgctgtcggcaacatctttcagacggccttcaaagcgtttggcgatgaaacctgaggcgcgggcggcgatgttgacgtatttgccgacgaggtcgctgtttacgcggctgataaagtcttgcaggttcaaatcgatgtcttcgattttgctgttgagtttggcggcgatgtagtagcgcatccactcggggttcaggccttgttccagataggatttggcggtaataaacgtgccgcgcgatttggacattttttgtccgtcgacggtcaaaaagccgtgtgcgtacacgccggtcggggcgcggtggccggagaaatgcagcatagcgggccagaacagggcgtggaaatagagaatatctttgccgatgaagtggtacatctcggtttggctgtcggctttgaagtattcgtcaaaatcgacgccgatgcggtcgcacaggtttttaaacgacgccatgtagccgacgggcgcgtccagccagacgtagaagtatttgcccggcgcgtcggggatttcaaaaccgaaatacggcgcgtcgcgggaaatatcccagtcggacagggtggtttcttcaccttcgcccagccattctttcattttgttgagggcttcggcttgcagatggggcttgccgtcgtgcgggttgttgccggaagtccatgctttgaggaagtcggcgcattcgcccagtttgaagaagaagtgttcggattcgcgcaattcgggtttcgtaccggaaacggcggaatacgggttaatcagttcggtcggggaataggtcgtgccgcagacttcgcagttgtcgccgtattggtcttgggcgtggcatttcgggcattcgcctttgacgaagcggtcgggcaggaacatttgtttttcggggtcgaaaagctgctcgatgacgcggctctcaatcttgccgttggctttcagcgcgcggtaaatgtcttgggaaaactgtttgttttcaggggaatgggtgctgtaataattgtcgtaaccgatgaaaaagccagtaaagtcggcgaggtgctcttcgcgcactttggcaatcatgtcttcgggcgcgataccttgtttttgcgcggcaagcattacgggcgtgccgtgggtgtcgtcggcgcagcagtagtggcacgcgtggccgcgcagtttttgaaagcgcacccaaacgtcggtttggatgtgttcgaccatgtggccgaggtggatgctgccgttggcatagggcagggcggaggtaactaagattttgcgtgtcatattgtgctttgcaaacaatgggtaaaggcggattataccgcaaatcaaacggggaaatgccgtctgaagcctgaaaaatcgggcttcagacggcatttttgccaaccggcgggagttattcgacggttacggatttcgccaggttgcgcggcttgtccacatcggtaccgcgtgcgagggcggtgtggtaggcgaggagctgcacggggatagtatgcacgacgggggacagtttgccgacgtggcgcggtgcgcggataacgtgcacaccttcggtggcattaaaattgctgtcgaggtcggcaaagacgaaaagttcgccgccgcgcgcgccgacttcctgcatattggctttgactttgtccaacaggctgtcgttgggtgcgatgacgacgacgggcatattttcgtccaccagggcaagcggcccgtgcttcagttcgccggcaggataggcttcggcgtggatgtaggtgatttccttcagcttcaacgcaccttcgagggcaatcgggtaatggatgccgcgccctaaaaacagcgcgctggttttcttggcaaactgttgcgcccatgcggcaatttgaggttcgaggttcagagcgtgctgcacgctgccgggaagctggcggagttcttcggtgtaacgcgcttcgtcttcttcggaaaccaaaccgcgcactttcgccagcgttaccgccaaaccgaacagcgcaaccagttgcgtggtaaacgctttggtcgaggcgacgccgatttccgcaccggcacgggtataaagcacgaggctgctttcgcgcggcagggcggattccatcacgttgcaaatggagaggctgtggcggtgtcccaaggatttggcgtatttcaacgcctccatcgtgtccagcgtttcgccggattgggaaatggtaatgaccagttggtcggaatcagcaatcacgctgcggtatcggtattcgctggcgatttcgacgtcggacgggatttttgcgatggattccaaccaatatttggcggtcagcgcggcgtaataggacgtgccgcaggcaaggattttgacgctgcggatgctttcaaacacgcttttggcatctttgccgaagttttcggggatgaagccgccgtcgaggaaaacctccgccgtgtctgcaatcgcgcggggctgctcgtggatttctttttgcataaagtggctgtacagtcccagttccaaagaggcgagcgagagttcggataccttgactttgcgttcggcaggcaggccgtttttatcggtcagccttttgatgccgtctgaagccagcagcgcgatgtcgccgtcttcgaggtacgccacgcggcgcgtaaaggcgatgacggcggatacgtccgaagcgataaaggtttcatcgtcgcccaaagcgaccaaaagcgggcagcccatacgcgccacaactaattcatcaggcttgtcttgggcaataaccgcgatggcgtatgcgccgtggaaacgtttgaccgcttcttgtaccgcttcaaacagcctgccgccgttttgcgcgtattcgtgattgatgctgtgtgcgatgacttcggtatccgtttgcgattcaaaacggtatcccaaaccttccaaacgtttgcgttcgctttcaaagttttcgatgatgccgttgtgtacgaccgcaatcataccgccgctgatgtgcgggtgggcgttcggctcagtaacgccgccgtgtgtcgcccaacgcgtatgtccgatgccgatgccgccgctgatgcctttttcgcgtgccgcgtcctccataagctgcacgcgtccgacgcggcgcacacgtttgattttgccgtcggtgttgacggcaatgcctgatgagtcataaccccggtattcgaggcgtttgagaccgtcggtcagaaaatcgacgacgttgtgatgggcgcggatggcgccgacgataccgcacataactgttccttagtatccggttgaaaaaaaacaggcgcggacggcttccgtgccgcaccttcctcttcggattataaaccgcctcccgcgccggaaaacagcaaaatgccgtctgaaggcttgggcttgctcaaaaaaaggagggatttccctgtttatccaggatgggcgttcagacggcattacctgctgctggtttatagtttttgcaaatcaacattgacaagctgaaaaaaaaaaacaatatactcgctcggtcttaatgttaacggagtatggaaatgaaacaaatgcttttagccgtcggcgtggtggcggtgttggcgggctgcggcaaggatgccggcggttacgagggttattggcgcgaaaagtcggacaaaaaagagggtatgattgccgtcaaaaaagaaaaaggcaattacttccttaataaaatccacgtggttacaggcaaggaagagtccttgcttttgtctgaaaaagacggcgcgctttcgataaacacagggataggggaaatcccgatcaaactttccgacgacgggaaagagctgtatgtcgaacgtaggcagtatgtcaaaaccgatgcggcgatgaaggacaaaatcatcgcccatcagaaaaagtgcggacaaacagcacaggcataccgcgacgcgcgaaatgcgttgccgtcaaaccagacgtatcagcagcatctggcggcgatcgagcaattgaaacggcggtttgaagccgagtttgacgaattggaaaaagaaatcaaatgcaacggcagaagcccggcattgttgctttagtaggggacaaccgggaggatgccgccgtccgaatcggatgtgcggtttctgtaccggtacgggcgggcaggaatgtccgccttttttgttcggatgcgtttgaatacccgtttgattccgaccgtttgcaaggggtatttccgttcgggcggaaattatagtggattaacaaaaaccagtacggcgttgcctcgccttagctcaaagagaacgattctctaaggtgctcaagcaccaagtgaatcggttccgtactatttgtactgtctgcggcttcgtcgccttgtcctgatttaaatttgatccactataattccgtcaaataagaaaggaattttgtgcctgcggtatcgcaaaacttcgccttaatgcgcccgattgcctagggatgggcttcagatggcattgttttccggtttacgggcggtattcgggcttcataccgttgggtaggagctgccagacatatcccgtggttttctgtttgccggcaagttcgccggcttcgtcgccgtatccccaaaaataatccacgcgcaccgcgcctttaatcgcgctgccggtatcctgcgccataatcaggcggttgagggctttgcgggtaaccggatgggcggtggcgacaaataagggcgcacccaaggtaatgtagtgccggtcgactgcgccggcatattcccccatcagcggcgtgcccagtgcgccgacagggccgtcattgctgcttccggcaagctcgcggaaaaagatatagctggggttttgacccaaaacttcggcgaggcgttgcggattttgccgcatataagacttaatgccctgcatggaggtttgtccgagtttgaggtagcccttatccgccatatagcgtccgatggaaacgtagggatgttcgtttttgtcggcatagccgatgcggatgtatttgccggacggggttttcagacggcccgagccttggatgtgcataaaaaaaagttcgacagggtcttcggcgtaaccgagtatcggggctttgccgtcaagcgcgccgccgttgatttggttgcgcgtgtggtaggggaggaagcggcttccttcaaacctgcctttgattgctgttgtgcgcgcggtgatggggaatcgggagaggtcggcggtatgtgtgccgccggtattgtcgattgtgccgctgttttttcccgtctgcctgatgcggacaagggcttttccgctccgcaaaccggcaggcagggggacggagataaaatcgtcgggaataccgtaaatcgggaagcgggcttgtgccgtccgcctgtcgtcgcccttcagcaccggttcgtaatagccggtaaccgtaccggcaaggcttccgttgcctgcaacctgccacggcgtgaaatagcgttcaaaaaactgttttgcctgaaaggaatggacgggggtttgaaaggcttgggcgcacacatcctgccagccttggcggtttttcaaattggcgcagccgaggcggaaggattgcaggcttttggcgaaatcctgcgccgcccagtggggcagggacaggtgcggtacaacggtatagacggccccgccgccgccgaccgtcgttccggcggggtcggggatgccgaccggccggtccgggccgttgatgacggatgtgtcgggttgcggaaaggtttggatgctcttgctttggcaggcggcgaggatggcggcggcgatgccgtacagggcggcgcggaataggtattttttcataatggacaatgttgccggcagtaataagaaagatggtttcgggcggcgttgcggcagccgtggagaggggattttaacacagggcgcagctgcagcctgcggaactttccgccgcgcggtactgcagataaaaataacttgcatttgtatttacaagcaatgaaaatattccgataatatattattcatcatccttgttcgttcgcgtttatgctggtcgcttttttaattatgttgcgcgagggtattgaagccgcgctcattgtcggcatcgttgccggttttctgaaacagtccggacattccaaactgatgcctaaggtctggttcggggtcgtccttgcttctttg

附录Batgtgtttggggctggggtacggcatccattcggcaacgggcgagattccccagaagcagcaggagttcgtcgtcggcattatcggtttggttgccgttgccatgctgacttatatgattttatggatgaaaaaggcggcgcgttcgatgaagcggcagcttcaggattctgtgcaggcggctttgaaccgtggcagcggtcaaggatgggccttggtcggtatggcgtttcttgccgtggcgcgcgaaggtctggagagtgtttttttcctgcttgccgtattcaaacagagcccgacgtggcagatgccggccggcgcggtagcgggggttttggctgccgccgtgattggcgcgttgatttatcagggcgggatgcgcctgaatctggcgaagtttttccgttggacgggggcgtttctgattgtcgttgccgccggcctgcttgccggctcgctgcgcgcgctgcatgaggcaggtatttggaacgcgcttcaggacattgtgttcgactcatcaaaatatttgcacgaagacagtccgttgggcgtgctgctcggcggatttttcggctataccgaccatccgacgcagggcgagaccttggtttggctgctgtaccttattcccgtcataacttggtttttgtgcggcagcaggccgtctgaaactttaacccgtaaagaggagctgaaatgagaaaattcaatttgaccgcattgtccgtgatgcttgccttaggtttgaccgcgtgccagccgccggaggcggagaaagctgcgccggcagcgtccggtgaggcgcaaaccgccaacgagggcggttcggtcagtatcgccgtcaacgacaatgcctgcgaaccgatggaactgaccgtgccgagcggacaggttgtgttcaatattaaaaacaacagcggccgcaagctcgaatgggaaatcctgaaaggcgtgatggtggtggacgagcgcgaaaacatcgcccccggactttccgataaaatgaccgtcaccctgttgccgggcgaatacgaaatgacttgcggtcttttgaccaatccgcgcggcaagctggtggtaaccgacagcggctttaaagacaccgccaacgaagcggatttggaaaaactgtcccaaccgctcgccgactataaagcctacgttcaaggcgaggttaaagagctggtggcgaaaaccaaaacttttaccgaagccgtcaaagcaggcgacattgaaaaggcgaaatccctgtttgccgacacccgcgtccattacgaacgcatcgaaccgattgccgagcttttcagcgaactcgaccccgtcatcgatgcgcgtgaagacgacttcaaagacggcgcgaaagatgccggatttaccggctttcaccgtatcgaatacgccctttgggtggaaaaagacgtgtccggcgtgaaggaaattgcagcgaaactgatgaccgatgtcgaagccctgcaaaaagaaatcgacgcattggcgtttcctccgggcaaggtggtcggcggcgcgtccgaactgattgaagaagtggcgggcagtaaaatcagcggcgaagaagaccggtacagccacaccgatttgagcgacttccaagccaatgtggacggatctaaaaaaatcgtcgatttgttccgtccgctgatcgaggccaaaaacaaagccttgttggaaaaaaccgataccaacttcaaacaggtcaacgaaattctggcgaaataccggactaaagacggttttgaaacctacgacaagctgggcgaagccgaccgcaaagcgttacaggcctctattaacgcgcttgccgaagaccttgcccaacttcgcggcatactcggcttgaaataagccgcaagcgttcagacggtatttggcggcagataccgtctgaagttttatagtggattaacaaaaaccagtacggcattgcctcgccttgccttgccgtactatttatactgtctgcggcttcgtcgccttgtcctgatttttgttaatccactatatccgccatatattgcagggcgggatttcaacctgccgctatcggttaatggaaaaacggcgtgcagggatacccatcctgctgcacggatattgaaggaaacaccatgagcaaaaaacaacccgcacaaccgaccaggcgcactctttttaaaaccgcgatcgcagccggagcagtcggcgcaatcggaggttatctcggcggcaaaaaacagggcgaaaccgccgaacgcaccgccgaaagccaacactcgccccaagcctatccctgctacggcgaacatcaggcaggcatcgttacgccgcagcaggcgttttcgattatgtgcgccttcgacgtaaccgcgcaaagtgccaagcagctggaaaacctgttccgcacgctgaccgcccgcatcgagtttctcacccaaggcggcgaataccaagacggcgacgacaaacttccgccagccggcagcggcattttgggcaaagccttcaaccccgacgggttgaccgttaccgtgggggtgggcagcagcctgtttgacggccggttcggactcaaagacaaaaaaccgattcatttgcaggaaatgcgcgacttctccaacgataagctgcaaaaaagctggtgcgacggcgatttgagcctgcaaatctgtgccttcacccccgaaacctgccaagccgccctgcgcgacatcatcaaacacaccgtccaaaccgccgttatccgttggagtatcgacgggtggcagcccaaatccgaacccggcgcgatggcggcgcgcaacctgttgggcttcagggacggcacgggcaaccccaaagtttccgatcccaaaactgccgacgaggttttgtggacgggggtggccgccaacagcctcgacgaaccggagtgggcgaaaaacggcagctatcaggcagtccgccttatccgccactttgtcgagttttgggacaggacgccgcttcaagagcaaaccgacattttcgggcggcgcaaatacagcggtgcgccgatggacggcaaaaaagaagccgaccaaccggattttgccaaagaccccgagggtgatatcacgcccaaagacagccatatacgcctggcgaatccgcgcgatcccgaattcctcaaaaaacaccgcctcttccgccgcgcctacagctattcgcgcggactcgcctcaagcggacagcttgatgtcgggctggtgttcgtctgctatcaggcaaaccttgccgacggattcatcttcgtgcaaaacctcctcaacggcgaaccgctggaagaatacatcagccccttcggcggcggctatttcttcgtcttgcccggcgtggaaaaaggcggctttttggggcaagggctgctgggcgtataaatccgccatataaaaaacgccgtccgaaccttgccaaacgggttcggacggcgtttcttgtttttgggcggtcaggcttttttgacgaattcggattttaaattcatcgcgctgccgtcgattttgcagccgatgttgtgatcgccttcttgcaggcgtatgcctttgacttttgtgccttgtttgatcaccatcgagctgccttttaccttgaggtctttgatgaggatgacggtatcgccgttttgcagcactgcgccgttggcatcgcgcacttgagccgcaaggtcggcggcggattcggtttcattccattcatgggcgcattcggggcagatgtattgtccgccgtcttcataggtgtattcggaggcgcattgcgggcatgggggtaatgacatggtttgccgtccttatcggatgtttgttttggggtgccgtctgaaacctgaaaccggcttcagacggcatagctttattgtttgtctttttcaggacgcacccagccttcgatgacggtttggcgggcgcgggcgagggcgagtttgttgtcttcgacattgcgggtaatcgtgctgcccgcgcctgtggttactttgttgccgagggtaacgggggcgactaggacgcagtttgaaccgatgcgcacttcgtcgccgatgacggttttgtgtttgtgcacgccgtcgtagttggcaataatcgtaccggcgccgaagttggttttgcagccgacttcggcgtcgccgatgtaggtgaggtggttggctttggtgcctttgccgatggcggcgtttttgatttcgacgaagttgccgacgtgtacgtcgtctgcaaggcgggcttgcggacgcaggcgggcgtacgggccgattcggttgttttcgccgacttcgcagctttcgaggtgggagaagggggcgattttgctgtttgcgccgattttggcgtttttgatgacgcagtttgcgccgatttcgacgttgtcgccgagctcgatgtcgccttcaaagatacagttcacatcaatcacgacgtcttgcccgtgtttcagacggcctcgtaaatcgaaacgtgccggatcgcgcagggttacgcctgctttgagcaattcttgcgcctgttcggtttggaagatgcgttcgagttcggtgagctggagtttgttgttcacgccggcggcgaggtgggaggcgcgcacttggacgggatgaactttaataccgtcggcaacggctttggcgatgaggtcggtcaggtagtattcgccttgtgcattgttgctggaaaggctgttcagccagttttcgagtttggcgttgggcaggacgaggatgccggtattgatttctttcacggctttttggacggcgtcggcgtctttttcttcgacgatggcggttacgctgccgttgctgtcgcggatgatacgccccaagcctgtcgggtcgttgggaacgtcggtcaacagcccgacttcgttgcctgcggcttcgagcagggtttcgagggtttcaacgtcaattaaaggaacgtcgccgtacaacaccagcgtgcggccttcggcggaaaggtggggcagggcggttttgacggcgtggccggtaccgagctgttcggtttgttcaacccaaacgacatcgcgtttgacggtgtccaagacttgctctttgccgtggccgatgacgacgcagatgttttgcggattcagtgcggctgcggtgtcgataacgcgcccgaccatgggcttgccgccgatgcggtgcagcacttttggcattttggaatacatgcgcgtgcctttgccggcggcgaggatgacgatgtttaaagtgttttgcggcatgacggtttcctgtgcaatgccgtctgaagcggcttcagacggcatagggtaggtttatcggttttgaaactttggtttttgccagtgttggcgatgctcttcgtcggcgttgttgccggtttgattgggtaacacggcatggcgttcgggacggtattggttgtagttcatatttttcgagtagctgccgtcttggtaataaacgggcgtgccggcgggatatttttgacggacggcggtcttgccgttgccgtcttgataagtttcccacgcgcagcccgacaaaagggcggcggcagcggtcaggaagaggaaggttttacgcatggcttttctttcgtattttcggggggtagggggtattgtaatgattttggcggtgttctgacaaagtttctgcataccgagccagttgcgccatatcgcttacggaggcatcgataaagggcagcgcgtgggattttgcaccgaaccggacggttttcatacccagcgcctttgcctgatgcaggttgtccgcgctgtcgtccaccataatgcagcattcgggcggtacgtccaacaggcggcagacattgagatacgcttgcggattgggtttgtacagcagcccgaaatcatccgtgccgaaaagcgcgtcgaaacggttttccaaaccgagtgcgttgacaacggcacggacgtaaaacgacgggccgttggaaaaaaccgccttgcgcccttttaggcggctcagggtgttttgtgtttcaggcatgccgtgcagcctggtcaggattgcatcgatcggatggctttcgcgcaaaaattcggcgatgtcgatttcgggatggtggatttgcagtccggcgagcgttgcgccgtagcggtgccaatagtcttgacgcaggtcggacgcggcagattcggagagtttgaggcggcgtgccatatagcgtgtcatagcgcggttgatgagtgtgaagatgcctgcgtcggcatcgtgcagcgtgttgtcgaggtcgaacagccacacggtcgggttttcttgcatgttgaaccgtgaaaatttgttagaatgttattttacagcgaatagaggaggactcggaatgaaacggaaaatttggctgctgccgctgctggcggtttcggcatacctgcaggcgcagacggaagtcaggctggcggtgcataagtcgttcagcctgcccaaagggttgattgcgcgcttcgagcgggcaaacgatgcgaaggtgtcgattattcaggcgggcggcgcgaacgaaatgctcaacaaactgattttgagccgcgccaacccgattgccgacgcggtgtatggtttggacaacgccaatatcggcaaggcgcgggaaatgggcattttggcggcggcgcaacccgaatccgcccccgtcgcggtcgggctgccttcggcttt

附录Bggcggtcgattacggctatgtgtccatcaattacgacaaaaaatggtttgaaggcaaaaagctgcccctgccgcaaaccctgcaggatttgacccgccccgaatataaaaacctattggtcgtgccgtcccccgccacgtcgtccccggggctgggcttcctgatggcgaacatcagcggtctgggcgaagaaagcgcgttcaaatggtgggcacagatgcggcagaacggcgtgaaggtcgccaaaggctggagcgaggcgtattacaccgacttttcgcacaacggcggcgcgtatccgctggtggtcggttatgccgccagcccggcggcggaagtgtatttttccaaaggcaaatacagcgagccgccgacgggcaacctgtttttaaaaggcggcgtattccgccaggtcgaaggcgcggcggtcttgaagggcgcgaaacagccggaattggcggcaaaactggtgcaatggctgcaaagtcgggaagtgcagcaggcggttccgtccgaaatgtgggtttaccccgccgtcaaaaacacgcgcctgcccgacgtgttccgcttcgcccaagccccgacgcacaccaccgcccccgcgcagcgcgatattgatgcgaaccagcgcggatgggtttcccgttggattagaacggttttgaaataaaacaaacatacctccccgcagggcttcatacggcatttttacacctgtgccgattacgccgcacggggcggatgttcgatcaagaggaaaacaatggacttcaaacaatttgattttttacacctgatcagtgtttccggttgggagcatctggctgaaaaggcgtgggcgttcgggctgaaccttgccgccgcgctgcttatttttttggtcggaaaatgggcggcgaaacgcattgtcgctgtgatgagggcggcgatgacgcgcgcgcaggtcgatgccacgctgattagttttttgtgtaatgttgccaatatcggcttattgattttggtgattattgccgcattgggcagattgggcgtttccacaacatccgtaaccgccttaatcggcggcgcgggtttggcggtggcgttgtccctgaaagaccagctgtccaattttgccgccggcgcactgattatcctgttccgcccgttcaaagtcggcgattttatccgcgtcggcggttttgaaggatatgtccgagagattaaaatggtgcagacttctttgcggacgaccgacaacgaagaagtcgtgctgcccaacagcgtggtgatgggcaacagcatcgtcaaccgttccacactgccgctgtgccgcgcccaagtgatagtcggcgtcgattacaactgcgatttgaaagtggcgaaagaggcggtgttgaaagccgccgtcgaacaccccttgagcgttcaaaacgaagagcggcaggctgccgcctacatcaccgccttgggcgacaatgccatcgaaatcacattatgggcttgggcaaacgaagcagaccgctggacgctgcaatgcgacttgaacgaacaagtggtcgaaaacctccgcaaagtcaatatcaacatcccgttcccgcaacgcgacatacacatcatcaattcttaaacgccgtctgaaagaggagtgggaaatggacgcgctgcacaccatcgcccgaaacctgacgaaaaaacgtcaaaccgtaagctgtgccgaatcctgtacgggcggaatgcttgccgccgcattcacaagcgttgcaggcagttcgcaatggttcgaccagagttttgtaacatacagcaacaaagccaaagaagaccgcttgggcgtgttgcccgaaaccctgctcgaacacggcgcggtcagccgccaaaccgtctatgagatggcgcgcggcgcgaaagccgtggcgcaggcggattacgccgtcggtatttccggcatcgccggtccgggcggcggcagcgaaagcaaacccgtcggcacggtttggttcgggtttgcctttccgggcggaagttgcgaagcaatgcgccgttttgacggcaaccgcgaatccgtccgcgcgcaggcggtcgccttcgcgttggaacggttggcggggctgattgaaaacggcggcgatgctgtctaaacaaaatctccgtctgaacaaaatccccatcggataaaaaatgccgtctgaaacgtttcgggtttcagacggcattttgtcggggtaggcggcggtgcggcttatttcactttacctttcaacgcgccatagcctgccgcgtccatttgttccagcgggatgaatttcaagctcgcgccgttgatgcagtagcgcagtccgcctttgtcgcgcgggccgtcggggaagacgtgtcccaaatgcgagtcggcggcgtggctgcgcacttcggtgcggcgcatgttgtagctgaaatcatcgtgttcggtaacggattttgcatcaatcgggcgcgtgaagctcggccagccgcagccggaatcatatttgtcggcggagctgaacaaaggttcgccgctgacaacgtccacataaatgccgggtttgaacaaatggtcgtattcgtggctgaaggcatattcggtcgcgctgttttgggtaacttggtattgctcttcggtcagggtgcgtttgagttcggcgtcactcggttttttatacgttgccgcgtcgaagcctttgccttgcggggcggtcttggttttgcccggcagcggttcgtcagctttgcggatgtcgatgtggcagtagccgttggggtttttaatcaagtagtcctgatggtattcctcggcatcgtagaagtttttcagcggctcgttttcaacaacgaggggcagttggtatttttgctgctcgcgtttgagggcggcggcgatgacggctttttcggcggggtcggtgtagtacacgccgctgcggtattgcgtaccggtgtcgttgccctgtttgttgaggctggtcggatcaacgacgcggaagaaatattgcaggatgtcgtctaggctgagtttgtcggcatcgtaggtcactttgacggtttcggcgtggcccgtatggcggtaggacacgtcttcatagctcggatttttcgtgttgccgttggcgtagccggataccgcgtcaaccacgccgtcgatgcgttggaaataggcttccaagccccagaagcagccgccggcgaggtaaatggtgcgcgtgttcatgatttttgaatcctttttctgagtgtcgggtttgtagaacgaatgtttcaagctgcccaaatcggcattcgggtcgcggattaacgccaacgcctgcgcttcgttgatgctgcctttgacgatgcgctgcacgtcgctgtctttaccgattaacgcccacgaggggtaaacgctgatattcaggctttgggcgatcgtgccgccgttgtcggttacgacgggcagcttgggataattcaaaccggcataccatttttggaagtcgccgtcttttttctcgtgcaaaaagcccggggaggcgacggtaatcaggttggcggagctgaattttgcatcttgcgcccatttttcggtctgtcccaattcggacagacacaaaggacaccagctcgcccaaaatttaatcagcgtcggtttgtcttttttcaagtaaacactggcggggcggttgtccgcagttttcaaagtggataaagtgtgcggcacggtcgcggctccggcatcgacgattttgggcgaacaagcacccagcgcaagcaggcagccgaacttggcgcaaagggaaaagaaagtacggtgtttcattttgatgtttcctgtgtggacggtttgcatgattagacgtttgagatgccgaaaccttacagcccggattttcagacaaccttaccgcgtaaaatacgctacaatacgccctgtttcaagtttctaaaattaaaaggaaaattcaatgttcagcttcttccgtcgcaagaaaaaacaggaaacgccggctctcgaggaggctcaaattcaggaaaccgcagcaaaagcagaatctgaacttgctcaaatagttgaaaatattaaagaagatgctgaatctttagcagaaagcgtcaaagggcaggtcgaatctgccgttgaaaccgtcagcggtgcggttgaacaggtaaaggaaaccgttgccgagatgctgtctgaagcagaggaagcggcggaaaaagcagcggaacaagtcgaagcggcaaaagaagccgttgccgaaaccgtcggcgaggctgtcgggcaagttcaagaagccgttgcgacaactgaagaacacaagctcggttgggcggcgcgtttgaaacaaggcctgaccaaatcgcgcgacaaaatggcgaaatcgctggcgggcgtgttcggcggcggacaaatcgacgaagatttatacgaagagctggaaaccgtgctgattaccagcgatatgggcatggaagccaccgaatacctgatgaaagacgtgcgcgaccgcgtcagcctcaaagggctgaaagacggcaacgaattgcgcggcgcgttgaaagaagccttgtacgacctgattaagcctctggagaaacctttggttttgcccgaaaccaaagagccgtttgtcatcatgcttgccggcatcaacggcgcgggcaaaaccacgtctatcggtaaactcgccaaatatttccaagcgcagggcaaatccgtattgctggcggcaggcgatactttccgtgccgccgcgcgtgagcagcttcaagcttggggcgagcgcaacaacgtaaccgtgatttcgcaaaccacgggcgattccgccgccgtgtgcttcgatgccgtccaagccgccaaagcgcgcggcatcgacattgtgctggccgacaccgccggccgcctgcccacgcagcttcatttgatggaagaaatcaaaaaagtgaaacgcgtgctgcaaaaagccatgcccgacgcgccgcacgaaatcatcgtcgtgcttgatgccaatatcgggcaaaacgccgtcaaccaagtcaaagcctttgacgacgcattggggctgaccggtttaatcgttaccaaactcgacggcacggcaaaaggcggcatcctcgccgcgcttgcctccgaccgccccgttcccgtccgctatatcggcgtgggcgaaggcatagacgacctgcgcccgtttgacgcgcgcgcgtttgtggacgcactgctggattgagccgaaatgccgtccgaaaacagcagaccgatgccgtcattcccgcgcaggcgggaatccagaccttgggataacggcaatattcaaaggttatctgaaagtccgagattctggattcccactttcgtgggaatgacgggatgtaggttcgtgggaatgacgtggtgcaggtttccgtatggatggattcgtcattcccgcgcaggcgggaatctagaacgtaaaatctaaagaaaccgtgttgtaacggcagaccgatgccgtcattcccgcgcaggcgggaatctagaccattggacagcggcaatattcaaagattatctgaaagtccgagattctggattcccactttcgtgggaatgacgggatttgagattgcggcatttatcggaaaaaacagaaaccgctccgccgtcattcccgcgcaggcgggaatctaggtttgtcggtgcggaaacttatcgggtaaaacggtttctttagattttgcgttctagattcccactttcgcgggaatgacgaagagttgcgggaatgatggaaagctatgggaataacgaagggttaaagtaatcacgggatggtgttcgcgggaatataaattgaaataattcaaaagggtattatatgcagcctgcggtttatattttagcaagccaacgtaatggcacgttatacattggcgttacatctgatttggtgcaacgtatttaccaacatagggagcatttgattgagggatttacatcacggtacaacgttactatgctggtttggtatgaactgcatcctacgatggagagtgcaattactcgggaaaaacagttgaagaaatggaacagggcttggaaattgcaactgattgaagaaaataatgtttcttggcaggatttatggtttgatattatttagcccgtcattcccgcgcaggcgggaatccggcttgttcggtttcggttttttttttgaggtttcgggcaacttctaaaccgtcattcccgcgtaggcgggaatctagaccttgggataacggcaatattcaaagtttataaaagacccgttattcccgcgcaggcgggaatctagaccttagaacaacagtaatattcaaaggttagctgaagctttagagattctagattcccactttcgtgggaatgacgggatgtaggttcgcgggaatgacgggatttgagattgcggcatttatcggaaaaaacagaaaccgttctgccgtcattcccgcgcaggcgggaatccggcttgttcggtttcggtttttttgaggtttcgggcaacttctaaaccgtcattcccgcgcaggcgggaatctagaccattggacagcggcaatattcaaagattatctgaaagtccgagattctagattcccactttcgtgggaatgacgggatgtaggttcgtgggaatgacgggatttgagattgcggcattt

附录Batcggaaaaaacagaaaccgctctgccgtcattcccgcgcaggcgggaatccggcttgttcggtttcggttttttttttttttgaggtttcgggcaacttctaaaccgtcattcccgcgcaggcgggaatccagaccattggacagcagcaatattcaaagattatctgaaagtccgggattctagattcccactttcgtgggaatgacgggatgtaggttcgtgggaatgacgggatttgagattgcggcatttatcggaaaaacagcaaccgctccgccgtcattcccgcgcaggcgggaatctagaccttgggataacagcaatattcaaaggttagctgaagctttagagattctggattcccactttcgtgggaatgacggaatgtaggttcgtgggaatgacgggatttgagattgcggcatttatcggaaaaacagcaaccgctccgccgtcattcccgcgcaggcgggaatctagaccttgggataacagcaatattcaaaggttagctgaagctttagagattctggattcccactttcgtgggaatgacggaatgtaggttcgtgggaatgacgggattagagtttcaaaatttattctaaatagctgaaactcaacgcactggattcccgcctgcgcgggaatgacgaattttaggtttctgattttggttttctgtttttgagggaatgacgggatttgagattgcggcatttatcgggagcaacagaaaccgctccgccgtcattcccgcgcaggcgggaatctagaccttagaacaacagcaatattcaaaggttagctgaagctttagagattctagattcccactttcgtgggaatgacggaatgtaggttcgtgggaatgacgcggtgcaggtttccgtatggatgggttcgtcattcccgcgcaggcgggaatccggcttgttcggtttcggttttttttttttgaggtttcgggcaacttctaaaccgtcattcccgcgcaggcgggaatctagaccttagaacaacagcaatattcaaagattataaaagacctgtcattcccgcgcaggcgggaatctaggtctgtcggcacggaaacttatcgggtaaacggtttcttgagattccgcgtcctggattcccactttcgtgggaatgacgggatgtaggttcgtgggaatgacgcggtgcaggtttccgtatggatgggttcgtcattcccgcgcaggcgggaatctagaccttagaataacagcaatattcaaagattatctgaaagtccgagattctggattcccactttcgtgggaatgacggaatgtaggttcgcgggaatgacgcggtgcaggtttccgtgaggatggattcgtcattcccgcgcaggcgggaatctagaccttagaacaacagcaatattcaaagattataaaagacctgtcattcccgcgcaggcgggaatccagaccttagaacaacagcaatattcaaaggttagctgaagctttagagattctggattcccactttcgtgggaatgacgggatgtaggttcgtgggaatgacgcggtgcaggtttccgtgcggatggattcgtcattcccgcgcaggcgggaatccagaccttgggataacagcaatattcaaaggttataaaagacccgtcattcccgcgcaggcgggaatctagaccttagaacaacagtaatattcaaaggttagctgaagctttagagattctggattcccactttcgtgggaatgacgggattagagtttcaaaatttattctaaatagctgaaactcaacgcactggattcccgcctgcgcgggaatgacgaattttaggtttctgattttggttttctgtttttgtaggaatgatgaaattttgagttttaggaatttatcggaaaaaacagaaaccgctccgccgtcattcccgcgtaggcgggaatccagaccgttgggcatctgcagcggtttgctaaaaaccgctttactgtgataagtgcgcagggttagaatggcgcggtaaccttatatattgtaccccgtcaaaggggcgcattgcttttcttaacattcccctttggcagccaagtgaaagggcttttcaatcagcaattcggcgggcgcggaatcgggcggtttaccgaaccccggcgttcgcggcgcgccgccccgtcccgtgaaggcaaactcaaggaataaaagatgaataaaacttggaaacggcaggttttccgccataccgcgctttataccgccatattgatgttttcccataccggcgggggggggggcaggcgcaagcgcaaacgcaaacgcaaacgcataaatacgctattgtaatgaacgcgcaaaatctgcccgaggtaaagtggggggatcaatatcagtcattgacgcacaaaagcaatgaacgcgaagttatccatacgagtggttttggtttggcaaaaaagagcattagtttctcattcaataataccgatgaagttgttgctgaaaaaaaagatactgtcgttttcggcgcggcgacctacctgccgccctacggaaaggtttccggttttgataccgctaagctgaccgagcgcaaaaatgcccttgatcagattggtacgaccaaaacggggctggtaggctacagctacgaaggtagcacatgctccagcggaggttgtcctacagttgcctatagaacccaatttaccttcggcaattccagtttggcaaaaaaggcaaacggcggcgggctggatatatacgaagacaaaagccgcgacaattcgcccatttacaaattgaaggatcatccttggttgggcgtgtctttcaatttgggcggagagagctccttcaaaccaaagagacaaggttctttggtatcttcttttagcgaggacgtgacgcagcaaaatggtgcgggcagccaacacaaagacaaaaacctcgtttatacgacagacgattacaagagtcagaataataaaaaccatcaggacaaacaccacgccgtcgccttttatctgaacgccaagctgcacctgctggataaaaaacacattaaaaatatcgtgcaaggtaaaacagttaatttgggtatcttgaaaacacgcatcgagccgacggaagcatggaaaagacggaatagtaacttttttaacggtagttggacgtatgaagagaaaggaacagtcagcgtcaaactcaaattgccggaagtcaaagcaggccgctgcatcaacgcaaataaccccaataagagtaccaaagccccttcccccgcactgactgcccccgcgctgtggttcggacctgtgcaaaatggtaaggtgcagatgtattccgcttcggtttccacctaccccgatagttcgagcagccgcatcttccttcaaaatctgaaaagaaaaaccgaccccaacaaacccggccgccattccctcgcagacttggctaagtcggatattgaaaatcgacagccgaatttcacagggcggcaaaccatcatccgattggatggcggcgtacagcagatcaaactgggtagaaacaatgatgaggtcgccaattttaatggaaatgacggcaaaaacgacactttcggcattgttagtgaagggagcttcatgcctgatgccagcgagtggaaaaaagtattgctgccttggacggttcgtgcttccaatgatgacggtcaatttaacacattcaacaaagaagaaaaagacggcaagccaaaatacagccaaaaataccgcagccgcgacaacggcaagcacgagcgcaatttgggcgacatcgtcaacagccccatcgtggcggtcggcgagtatttggctacttccgccaacgacgggatggtgcatatcttcaaacaaagcggcggggacaagcgcagctacaatctgaagctcagttatatcccgggtacgatgccgcgcaaggatattcaaaacaccgaatccacccttgccaaagagctgcgcgcctttgccgaaaaaagctatgtgggcgaccgctacggcgtggacggcggctttgtcttgcgcaaagtcgaacggaacgggaaagaccatgtgtttatgttcggcgcgatgggctttggcggcagaggcgcgtatgccttggatttaagcaaaatcgacagcggcaacggcaacctggcagacgtttccctgtttgatgtcaaacatgacaagaatggcaataacggcgtgaaattaggctacaccgtcggcacgccgcaaatcggcaaaacccacgacggcaaatacgccgctttcctcgcctccggttatgcgactaaagacattaccagcggcgacaataaaaccgcgctgtatgtgtatgatttggaaagcagcggcacgctgattaaaaaaatcgaagtacccggtggcaagggcgggctttcgtcccccacgctggtggataaagatttggacggcacggtcgatatcgcctatgccggcgatcgcggcggcagtatgtaccgctttgatttgagcaatcaagatcctaatcaatggtctgtacgcgccatttttgaaggcacaaaaccgattacttccgcgcccgctatttcccaactgaaagacaaacgcgtggttatcttcggcacgggcagtgatttgagtgaggatgatgtactcagtacgagcgaacaatatatttacggtatcttcgacgacgatacggtggcgaataacgtaaatgtaaaactcagcggtttgggaggcgggctgctcgagcaagagcttaagcaggaggataaaaccttattcctgaccgattacaagcgatccgacggatcgggcagcaaagggtgggtagtgaaattgaagggcggacagcgcgttaccgtcaaaccgaccgtggtattgcgtaccgcctttgtaaccatccataaatatacgggtacggacaaatgcggcgcggaaaccgccattttgggtatcaataccgccgacggcggcaagctgaccaagaaaagcgcgcgcccgattgtgccggccgagaatcaggctgtcgcgcaatattccggccataagaaaggcatcaacggcaaatccatccctataggttgtatgcaaaaaggcaatgaaatcgtctgcccgaacggatatgtttacgacaaaccggttaatgtgcgttatctggatgaaaagaaaacagacggattttcaacaacggcagacggcgatgcgggcggcagcggtatagaccccgccggcaagcgttccggcaaaaacaaccgctgcttctcccaaaaaggggtgcgcaccctgctgatgaacgatttggacagcttggacattaccggcccgacgtgcggtatgaaacgaatcagctggcgtgaagtcttctactgatttgcacgcgaaaatgccgtccgaaaggttttcggacggcattttttgcgtttttcgggaggggcgggttcgtaaaaggcgggctatagggtaggcttcatctcgccaatctcactgaatccatcaatttccacaattcaattaaataccgtcaaaccgatgccgtcattcccgcgcaggcgggaatctagaccttagaacaacagcaatattcaaaggttagctgaagctttagagattctggattcccactttcgtgggaatgacgggatgcaggtttccgtatgaatggattcgtcattcccgcgcaggcgggaatccagaccttagaacaacagtaatattcaaagattatctgaaagtccgagattctggattcccactttcgtgggaatgacgggattttaggtttctgattttggttttctgtttttgtaggaatgatgaaattttgagttttaggaatttaccggaaaaaacagaaaccgttctgtcgtcattcccgcgcaggcgggaatctagacattcaatgctaaggcaatttatcgggaatgactgaaactcaaaaaactggattcccactttcgtgggaatgacgggatttgagattgcggcatttatcgggagcaacagaaaccgctctgccgtcattcccgcgcaggcgggaatccagaccttagaacaacagtaatattcaaagattatctgaaagtccgagattctggattcccgcctgcgcgggaatgacgaattttaggtttctgattttgtttttctgtttttgtgggaatgatgaaattttgagttttaggaatttatcggaaaaaacagaaaccgctctgccgtcattcccgcgcaggcgggaatctagaccttagaacaacagcaatattcaaagattatctgaaagtctgagattctagattcccactttcgtgggaatgacgggatgtaggttcgtgggaatgacgtggtgcaggttcgtgggaatgacgtggtgcaggttcgtaggaatgacgtggtgcaggtttccgtgcggatggattcgtcattcccgcgcaggcgggaatctagaccttagaacaacagcaatattcaaaggttatctgaaagtccgagattctggattcccactttcgtgggaatggcgcgattagagtttcaaaatttattctaaatagctgaaactcaacgcactggattcccgcctgcgcgggaatgacgaagtggaagttacccgaaacttaaaacaagtgaaaccgaacgaaccggattccc

附录Bactttcgtgggaatgacgggatgcaggtttccgtacggatggattcgtcattcccgcgcaggcgggaatctagacattcaatgctaaggcaatttatcgggaatgactgaaactcaaaaaactggattcccactttcgtgggaatgacgggattagagtttcaaaatttattctaaatagctgaagctcaacgcactggattcccgcctgcgcgggaatgacgaagtggaagttacccgaaacttaaaacaagcgaaaccgaacgaactggattcccattgtcgtggaaatgacgggattttaggtttctgtttttggttttctgttttcgtgggaatgacgggatgtaggttcgtgggaatgacggttcagttgctacgcatttaccctgcgcaaagctttatccactatcttgtaacctgtctgacaatctgtcctctcttacaaaatgccgaaactttttcaggctgcattttggggctgcctgtgcggaatttggcggtaggcgcggtagtagggttcgagctgtcgggcgatgagttggagctgttggaggaggatgtggctttgtgttccgctgctgtgggtgcggagggtgtcgagttcgccgcgcagtgtatccagtgctgtctgaaagtcgtcgggttcggtttcgggcaggtgttggaagatgtgggcggtgtgttcggcggcgaggtggaactgtgcggtaaagtcggggctgcattcttcgtgcatttcgctgcggtatgcgccgagggcggagatgtagccggtcagggcgtagccggttttgagcagggtaaagccgggttgcaggctgtcggcgaattttgcgggttcgctgctcatgtcggaaagggtgctgctgagggcggcggtgtgttcgtgggcgcggcggcgggtggcgcggtattcgacgtcgtcgccggtttcgccgcttttgaggcgttcggtgattttttcgagataggcaccgttgctgcatacggcaagggcggcggtgcgttcgagcgtgaggtatttccagtctggccacaggtagctgactgccgcccaggcaagggatgcgccgataatggtgtcgatgatgcgtacgggcatggcggcgtatacgtccaaacctgcgagggagaggctggtcagggcttgaatggtaatgaagaaggtggagaaactgtatttgtaggtgcgggtcatgaaaaagagggtggtactggcgatgacaatccagagtttggtttcgacagacggggtgaagtaggggacgagcgagccgacgattacgccgagtacggtgccggcgatgcgctggcggacgcggcttttggtggcggtgtagttgggttggcagacgaaaagggcggtcagtagtatccagtagccgaggttgaggttgagggcttcgacgatggtgcaggcggcggcaacgacgagggacaggcggacggcatggcggaatacgcctgattcgaggtttagctgcggacggattgcctgccaggtgtttttgaggctgctggtttcgagggcggcgatgcgggtgtcgcccatgcggtcgttttctgcctgcaggccgttgtgctggagttggcggaactgctggtcgacgctgccgaggttgtcgagaaggcggcgcaggtggcggatgtcgggactgtcgttgctgtctgaaaggaggcgcagcgattggcggcagccttcgatggcgcggccgaggcgtttgctgtaaacgtagtctttgcttgcgcgcagggcttgggcggtgttgcggcaggcttgtccctgcatttcgagcaggcggtggatgcggaagatgatgtcggtgtttttgaatttttcggacatttcctgataatcgacgtgggcggagctgatgcgttcgtgtatgtcttgggcggcaaagtagtaacgcagcattttggcggtgcgcgggtggcggtgtttgccgcgaaggcggtaaaacagggcggaacggcattggttgaaggcggtgatgacgccggtgttgctcatggcgaggtcgatgtggcggttgcctatccaggctgcctcatcggggtcgaagaagtcggctttggcttcgaggtagccgccgagtgcgtcgtaggcgttggcgacgctttcttggacggggcggtggggcaggacgatttggaacaggaggatggcggtgctgtacagtacggtgccgcataaaatcatgaaggggttggtcagccagtaggtttcgggggtgtaggtaagtgtggtgtaggtggcgacggcgagtgcaccgaaggcgaaggtgcggtatttgagcccgaccgcgcctaaaatggtgaagccgaaggtcatcagggtcatggcgaggatgaagggcagccctgtgccgagggtgctttgtgccgtgagcgaggagagggtgaacagggcgacggtggtgatgatgtttttcagccgtccggtcaggcggttgtccaaatcgacaaggccgccggcgatgatgccgagtacgaagggcatggcgagcttgggttcgcctagctgccagacgatggaggcggcggtaaaaacactggcgaaaacgggaagcgaggtaatgagcagaggcttgaggagtggggttttcatggttttaccggtttattgttatgaagtgaatatagtggattaacaaaaaccagtacggcgttgcctcgccttagctgaaagagaacgattctctaaggtgctcaagcaccaagtgaatcggttccgtactatttgtgctgtctgcggcttcgtcgccttgtcctgatttttgttaatccactataaatttaatccactataaagtgtagcacatgaatggggcggataaaatcatgccgtctgaaaacggggatgcggttttcagacggcattgggttttgcggatcaggaaatgaggttgagaccgttgaccctgtcgtaaaggagttcgggcgttttgccttctttgtgcagttggatgtgcaatcgcaggttgttggcggaaacggactggcgcagggcttcttcgtaactgatgatgccgtgacggtacagttcgaaaaggttttgatccatcgtctgcattccgtcggttttggcggtttccatgattttactgatgttcatcaggtcgcccttcaggatgaagtcttggatggcgggcgtgttgatgagcaagtcgacaaccgccgtcctgcccgttttgtcttgtttgagggcgaggcgttggcagatgatgccggtcaggttgagggcgatgtcgatcagtatttggttgtgctgttctttggggtagaagttgagtatgcgttcgagcgactgcggcgcggtgttggcgtggagcgtaaaaatgcacaggtggccggtttgggcgagctgcatcgcgtattccatactttccctgctgcggacttcgccgatgcagaccacgtcgggggattggcgcatagcgttttgtaccgccgtctgccagtttatggtgtcgacgccgatttcgcgctgggtaaagatgcagcggcgcggtttgtagataaattcaatcgggtcttcgatggtaacgatatggctgggcagggttttgttgcggtgttcgagcatagtcgccatcgtggtggatttgcccgaaccggtaggcccgacgataatcagcagcccgcgcggtgcgacggcgaggtctttgagtttttcgggcaggcccaattcctgcatttgcgggatgacgtggttgatgcgccgcaaaaccaaacctgcgctgccttggctgtggtaggcgttggcgcggtagcgcgtgccgctgcgcgactggacggagtagttgatttcgccgtcgcgccggaatatttccgattgttcggcgttcatcgtcgatgcggcgatggcggcggtttcctcgcccgtcagcgccttttgcggctgcggggttaatgcgctgttgattttcaacgagggcgggaatcctttgctgataaggatgtcggacgcgttttgtgcttctgcggtttcgcacaggcggtcgagcagcgggtggaagtgtgcgccgatttcggccggggtttcggatcggctttgttttttttgagaatacacttgaaccatttcgtccaagatgtcgtgcaggttatcggtattcatcgttagcttcttttcggtttaagccttgcagtttgcggcggcaggtttcaacaggaaggcggacgcttcttgttcggaaaggtagccgggcgggatgctgcgtcccgccccgcgtgtttgcgccttgttttcccgccggtatggccggaaagcggttgtgtgtcagaaactcatactttcgctgttttgcgcgcgtctgcgtgcgacttccggtgcgatcagcccttggcgcaccagcgattgcagcgattggtccattgtctgcataccgctcgcctgcccggtttgcaggacggagttaatctgcgtgattttgttttcgcggatgaggttgcggacggcggggttggcaatcaggatttcgtgcgaggcgacacggccgttgccgtcgtgcgttttcagcaggttttgggagatgacggcggtcagcgattcggacagcatagagcgcaccatttctttttctcccgccgggaatacgtccacaatacggtcgacggtttttgctgcgccggtcgtgtgcagcgtgccgaaaaccaagtgtccggtttcggcggcggtcagtgccaagccgatggtttctgggtcgcgcatctcgccgacaaggataacgtcggggtcttcgcgcaatgcggaacgcagcgcgttggcgaagctgagggtgtgctggtgcagctcgcgctggttaatcagggattttttgctttggtggacgaattcaatcgggtcttcgatggtcaggatgtgtgccggctgggtttcgttgatgtagttgatcatcgcggcaagcgtggtcgatttgcccgaaccggtagggccggtaaccaaaaccatgccgcgcggcgattctgcgattttttggaaaatgctcggggctttcaattcttccagcgataagacggtgctgggaatggtgcggaatacggcggcgggaccgcggccgatgttgaaggcgttgacgcggaatcgggcgacgttgggcagttcgaacgagaagtcgacttccaagttttgctggtagattttccgctggtggtcgttcatcaccgaagttaccatattaccgacctcttccgcgctcatttcgggaaggttgatgcgccgcatatcgccgtgaacccgaatcataggggatatgcccgaactcaggtgaaggtcggatgctttgtttttagcgccgaaggcgagtaagtcggtaatctgcataatgcggctctgtttagtataatgtttcgattggttggaatggttctaacaaccttgattgtaccgccctgactggaggggtttcaactgtttaatcatttttaattaggggataatctatgacggtgttgcaagaacgttattgtgaggtgtccgaccgtatcggaaaattggttctgcaggcgggcagggagccgcattccgtcagcctgattgccgtcggtaagactttcccttcagacggcatccgcgaagtttacgccgccggacagcgtgatttcggcgagaactatattcaggagtggtacggcaaaacggaagagttggcggatttgaccgacatcgtgtggcacgtcatcggcgatgtgcagtccaacaaaaccaagtttgtcgccgaacgcgcgcattgggtgcataccgtatgccgtctgaaaaccgccgtccggctgagcgggcaacgtccttcctcaatgccgcctttgcaggtgtgtatcgaggtgaacattgcgggcgaggcggtgaagcacggtgtcgcgcccgaagaagcagtcgcgcttgctgtggaagtggcgaagctgccgaatatcgtcgtacgtggactgatgtgtgttgccaaagccaacagcagtgaaacggagttgaaggtgcaatttcaaacgatgcggaaactgcttgccgacctcaatgcggctggcgttaaggcagacgtgctgtctatggggatgtcggacgatatgcctgccgccattgagtgcggtgcgacacacgtccgtatcggcagcgcgattttcgggaaaaggggctgatggaaattcgggcaataaaatatacggcaatggctgcgttgcttgcatttacggttgcaggctgccggctggcggggtggtatgagtgttcgtccctcaccggctggtgtaagccgagaaaaccggctgccatcgatttttgggatattggcggcgagagtccgccgtctttaggggactacgagataccgctttcagacggcaatcgttccgtcagggcaaacgaatatgaatccgcacaacaatcttacttttacaggaaaatagggaagtttgaagcctgcgggctggattggcgtacgcgtgacggcaaacctttgattgagacgttcaaacagggaggatttgactgcttggaaaagcaggggttgcggcgcaacggtctgtccgagcgcgtccgatggtaaaaaattgggaatgaatttagtaaggtaattttgaatagggtagaaataatgaatgtttattttctcggcggcggcaatatggcggctgccgttgcgggcggattggtcaaacaagg

附录Bcggttaccgcatctatatagccaatcggggtgcggaaaaacgcgaacgtttggaaaaagagttgggggtcgaaacttcggcaaccctgccggagcttcattccgacgatgttttaatccttgccgtcaaaccgcaggatatggaagctgcgtgcaaaaatatccgcaccaacggcgcattggtgctttctgtcgcagccggattgtcggtcggtacgctcagccgttacctcgggggaacacgccgcattgtccgggttatgccgaatacacccggaaaaatcgggctgggcgtatctggtatgtatgccgaagcggaagtatcggaaacagaccgcaggattgccgatcgaatcatgaaatcagtcggtttgactgtttggttggatgatgaggaaaaaatgcacggcattaccggcatcagcggcagcggaccggcttatgtgttttatctgctggacgcattgcaaaatgccgccatccgacaagggtttgatatggcagaagcacgcgcgctcagtctggcaacgtttaaaggagcggttgcccttgccgagcagacgggtgaagatttcgagaagcttcaaaaaaatgtaacgtcaaaaggcgggacaacccacgaagccgtggaagctttcaggcggcatcgtgtcgccgaagccataagcgagggcgtttgtgcctgtgtgcgccgttcgcaggaaatggaacggcaatatcaataatgtaaagaaaataaaaaaaccaatccaaaacgtgttatgatgcgcgttttcaaaaacgccttaggcaataagccttataaaaatcaaaggaataaagccactttgtggtgctttgttttttgcggtgaaccgagaggatatacattatggcaaagctgacagaacaagatattttgaattggagcgggccggaagacgattatatgaatgacgaccatttggcttttttccgcgaattgctggtaaaaatgcaagacgaactcatcgaaaatgcttccgctacgacagggcatctccaagaacacgaatcagcccccgatcctgccgaccgtgccacacaggaagaagagtacgcattggaactccgtacccgcgatcgggaacgaaaacttctcagtaaaatacaggcgaccatccgcaatattgatgaaggggattatggattctgtgccgatacgggagagcctatcggtttgaagcggctgctggcacgcccgacagccactttatctgttgagtcccaagaacgccgagagaggatgaaaaaacagtttgccgactgatggcggcaaacaaaatgccgtctgagtccccgagtttcagacagcatattcacaaaggcgcaccagccggaggagggagaggaaaggattgttggaggcggcgcagtatttagcagaaataaaaaaccttatccgacagcgacatgacgaatttccccaaaaaaatcccgctgaaagcattgaccgtttttccctgtgggcgtatagttcggttcttcgctgctgcagaagtggcggacgaactgaaaagtatagcacagaatgttggggatatcgagagatatcttgacaggcggaaggaatactttataattcgcaacgctctttaacaaaacagattaccgataagtgtgagtgccttgagtctcacactgtttgaaagacagacaagataatgttttgaacattgtcctgttggtttctttgaagcagaccagaagttaaaaagttagagattgaacataagagtttgatcctggctcagattgaacgctggcggcatgctttacacatgcaagtcggacggcagcacagagaagcttgcttctcgggtggcgagtggcgaacgggtgagtaacatatcggaacgtaccgagtagtgggggataactgatcgaaagatcagctaataccgcatacgtcttgagagagaaagcaggggaccttcgggccttgcgctattcgagcggccgatatctgattagctagttggtggggtaaaggcctaccaaggcgacgatcagtagcgggtctgagaggatgatccgccacactgggactgagacacggcccagactcctacgggaggcagcagtggggaattttggacaatgggcgcaagcctgatccagccatgccgcgtgtctgaagaaggccttcgggttgtaaaggacttttgtcagggaagaaaaggctgttgctaatatcagcggctgatgacggtacctgaagaataagcaccggctaactacgtgccagcagccgcggtaatacgtagggtgcgagcgttaatcggaattactgggcgtaaagcgggcgcagacggttacttaagcaggatgtgaaatccccgggctcaacccgggaactgcgttctgaactgggtgactcgagtgtgtcagagggaggtagaattccacgtgtagcagtgaaatgcgtagagatgtggaggaataccgatggcgaaggcagcctcctgggacaacactgacgttcatgcccgaaagcgtgggtagcaaacaggattagataccctggtagtccacgccctaaacgatgtcaattagctgttgggcaacctgattgcttggtagcgtagctaacgcgtgaaattgaccgcctggggagtacggtcgcaagattaaaactcaaaggaattgacggggacccgcacaagcggtggatgatgtggattaattcgatgcaacgcgaagaaccttacctggtcttgacatgtacggaatcctccggagacggaggagtgccttcgggagccgtaacacaggtgctgcatggctgtcgtcagctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccttgtcattagttgccatcattcagttgggcactctaatgagactgccggtgacaagccggaggaaggtggggatgacgtcaagtcctcatggcccttatgaccagggcttcacacgtcatacaatggtcggtacagagggtagccaagccgcgaggcggagccaatctcacaaaaccgatcgtagtccggattgcactctgcaactcgagtgcatgaagtcggaatcgctagtaatcgcaggtcagcatactgcggtgaatacgttcccgggtcttgtacacaccgcccgtcacaccatgggagtgggggataccagaagtaggtaggataaccacaaggagtccgcttaccacggtatgcttcatgactggggtgaagtcgtaacaaggtagccgtaggggaacctgcggctggatcacctcctttctagagaaagaagaggctttaggcattcacacttatcggtaaactgaaaaagatgcggaagaagcttgagtgaaggcaagattcgcttaagaagagaatccgggtttgtagctcagctggttagagcacacgcttgataagcgtggggtcggaggttcaagtcctcccagacccaccaagaacgggggcatagctcagttggtagagcacctgctttgcaagcagggggtcatcggttcgatcccgtttgcctccaccaatactgtacaaatcaaaacggaagaatggaacagaatccattcagggcgacgtcacacttgaccaagaacaaaatgctgatataataatcagctcgttttgatttgcacagtagatagcaatatcgaacgcatcgatctttaacaaattggaaagccgaaatcaacaaacaaagacaaagcgtttgttttgattttttattctttgcaaaggataaaaatctctcgcaagagaaaagaaaacaaacacagtatttgggtgatgattgtatcgacttaatcctgaaacacaaaaggcaggattaagacacaacaaagcagtaagctttatcaaagtaggaaattcaagtctgatgttctagtcaacggaatgttaggcaaagtcaaagaagttcttgaaatgatagagtcaagtgaataagtgcatcaggtggatgccttggcgatgataggcgacgaaggacgtgtaagcctgcgaaaagcgcgggggagctggcaataaagcaatgatcccgcgatgtccgaatggggaaacccactgcattctgtgcagtatcctaagttgaatacatagacttagagaagcgaacccggagaactgaaccatctaagtacccggaggaaaagaaatcaaccgagattccgcaagtagtggcgagcgaacgcggaggagcctgtacgtaataactgtcgagatagaagaacaagctgggaagcttgaccatagtgggtgacagtcccgtattcgaaatctcaacagcggtactaagcgtacgaaaagtagggcggggcacgtgaaatcctgtctgaatatggggggaccatcctccaaggctaaatactcatcatcgaccgatagtgaaccagtaccgtgagggaaaggcgaaaagaaccccgggaggggagtgaaacagaacctgaaacctgatgcatacaaacagtgggagcgccctagtggtgtgactgcgtaccttttgtataatgggtcaacgacttacattcagtagcgagcttaaccgaataggggaggcgtagggaaaccgagtcttaatagggcgatgagttgctgggtgtagacccgaaaccgagtgatctatccatggccaggttgaaggtgccgtaacaggtactggaggaccgaacccacgcatgttgcaaaatgcggggatgagctgtggataggggtgaaaggctaaacaaactcggagatagctggttctccccgaaaactatttaggtagtgcctcgagcaagacactgatgggggtaaagcactgttatggctagggggttattgcaacttaccaacccatggcaaactaagaataccatcaagtggttcctcgggagacagacagcgggtgctaacgtccgttgtcaagagggaaacaacccagaccgccagctaaggtcccaaatgatagattaagtggtaaacgaagtgggaaggcccagacagccaggatgttggcttagaagcagccatcatttaaagaaagcgtaatagctcactggtcgagtcgtcctgcgcggaagatgtaacggggctcaaatctataaccgaagctgcggatgccggtttaccggcatggtaggggagcgttctgtaggctgatgaaggtgcattgtaaagtgtgctggaggtatcagaagtgcgaatgttgacatgagtagcgataaagcgggtgaaaagcccgctcgccgaaagcccaaggtttcctgcgcaacgttcatcggcgtagggtgagtcggcccctaaggcgaggcagaaatgcgtagtcgatgggaaacaggttaatattcctgtacttgattcaaatgcgatgtggggacggagaaggttaggttggcaagctgttggaatagcttgtttaagccggtaggtggaagacttaggcaaatccgggtcttcttaacaccgagaagtgacgacgagtgtctacggacacgaagcaaccgataccacgcttccaggaaaagccactaagcttcagtttgaatcgaaccgtaccgcaaaccgacacaggtgggcaggatgagaattctaaggcgcttgagagaactcaggagaaggaactcggcaaattgataccgtaacttcgggagaaggtatgccctctaaggttaaggacttgctccgtaagccccggagggtcgcagagaataggtggctgcgactgtttattaaaaacacagcactctgctaacacgaaagtggacgtatagggtgtgacgcctgcccggtgctggaaggttaattgaagatgtgagagcatcggatcgaagccccagtaaacggcggccgtaactataacggtcctaaggtagcgaaattccttgtcgggtaagttccgacccgcacgaatggcgtaacgatggccacactgtctcctcctgagactcagcgaagttgaagtggttgtgaagatgcaatctacccgctgctagacggaaagaccccgtgaacctttactgtagctttgcattggactttgaagtcacttgtgtaggataggtgggaggcttagaagcagagacgccagtctctgtggagccgtccttgaaataccaccctggtgtctttgaggttctaacccagacccgtcatccgggtcggggaccgtgcatggtaggcagtttgactggggcggtctcctcccaaagcgtaacggaggagttcgaaggttacctaggtccggtcggaaatcggactgatagtgcaatggcaaaaggtagcttaactgcgagaccgacaagtcgagcaggtgcgaaagcaggacatagtgatccggtggttctgtatggaagggccatcgctcaacggataaaaggtactccggggataacaggctgattccgcccaagagttcatatcgacggcggagtttggcacctcgatgtcggctcatcacatcctggggctgtagtcggtcccaagggtatggctgttcgccatttaaagtggtacgtgagctgggtttaaaacgtcgtgagacagtttggtccctatctgcagtgggcgttggaagtttgacgggggctgctcctagtacgagaggaccggagtggacgaacctctggtgtaccggttgtaacgccagttgcatagccgggtagc

附录Btaagttcggaagagataagcgctgaaagcatctaagcgcgaaactcgcctgaagatgagacttcccttgcggtttaaccgcactaaagagtcgttcgagaccaggacgttgataggtggggtgtggaagcgcggtaacgcgtgaagctaacccatactaattgctcgtgaggcttgactctatcatttgaagaacttcaagagataaaagcttactgactgattcagtcattaccgaatatattgattaaggctttaccgatttgtaacagtttaagtttggcggccatagcgagttggtcccacgccttcccatcccgaacaggaccgtgaaacgactcagcgccgatgatagtgtggttcttccatgcgaaagtaggtcactgccaaacacccattcagaaaacccccgattattcgggggtttttgctttgcccggaaaaaatgtttgctttgcccggaaaaaatgtcggtgatggcgggacggcatccgtacggtgtccggtcgggtttgcggaggaacggcttgaaactttgggatattcattttagaatgactcgttttatcgtcgcaagatgcggtttattgtttgcaacccttaaaggaaaaaccatgaagaaaatgttcgtgctgttctgtatgctgttctcctgcgccttctcccttgcggcggtaaacatcaatgcggcttcgcagcaggagttggaggcgctgccaggcataggccctgcggtgctggcgaagctgaaggatcaggcttccgtcggcgcgcccgcaccaaaaggcccagccaaaccagtgctgcccgcggataaaaaataaaatagggggaagtctgcagccgcatcaaatgccgtctgaacatgcgttcgggcggcgtttttataacaaaaacacttcatggcggttggttttatgcctatctaagtttttgtgtcgtgcatacctgaagatttcagacggcatcggtttatgctgtctgaaaagtgtattccgtttcagtttgtaagctatggcagtctgtttgtcttgtgttttgcgcaattgcccttattttgagccgtgattttattttgaattagatgaaaaaatgagtaatcaagatttttatgcgacgctgggtgtggcaagaacagctaccgatgatgagattaaaaaagcctaccggaaattggcgatgaaataccatcccgaccgcaatcctgacaataaagaggcggaagagaagtttaaagaagtacaaaaggcgtatgaaactttgtccgacaaggaaaagcgcgctatgtacgaccagtatggtcatgcggcgtttgaaggcggcggacaggggggcttcggagggtttggcggatttggcggtgcgcagggttttgactttggggatattttcagccaaatgtttggaggcggttcggggcgcgcccagcctgattatcagggtgaggacgttcaagtcggtatcgaaatcacgcttgaagaagccgcaaaaggtgtgaagaaacgcatcaatattccgacttatgaagcgtgtgatgtctgtaacggcagtggcgcgaaaccggggacatccccggaaacctgcccgacttgcaaaggttcgggtacggtgcacatccagcaggcgattttccgtatgcagcagacttgtccgacctgccacggtgcgggcaaacacattaaagaaccttgcgtcaaatgccgtggcgcggggcggaataaggcggtcaagacggtggaagtcaatattcccgccggtatcgatgacgggcagcgtatccgtttgagcggcgaaggcgggccgggtatgcacggtgcgcctgccggcgacttgtatgtaaccgtccgcattcgggcgcataagattttccaacgcgacggtctggacttgcattgcgaactgccgatcagttttgccacggctgctttgggcggggagttggaagtgccgaccttggacggaaaggtcaagctcaccgtccccaaagaaacccaaaccggcaggaggatgcgcgtgaagggtaagggtgtcaaatctttacgcagcagcgcgaccggcgatttgtactgccatattgttgtcgaaacgcctgtcaatttgaccgaccgtcaaaaagagcttttggaagaatttgagcggatttctaccggcttggaaaaccaaacaccgcgcaagaaatcgtttttagacaagctgcgcgatttgtttgattgattttaaggttcggaaacaagcagccgtatcggggaatctccttgatacggctgtttttatttgtttaaaaatagtttttattttcaatggggtatgaggcagggtgggataactgtttttaactgttctttttaaaacttgacatcatggcgtgatgccaacaatatgtgaacgtctgttgtcaaaggaagaataatgaataaatctttatccagttcggtagaagaataccgcgagctgacgctccgaggcatgatactcggtgcattgatcactgtaatttttactgcgtccaatgtttacctcggtttgaaagtcgggctgacctttgcctcgtcgattccggcggcggtgatttcgatggcggttttaaagtttttcaaaggcagcaatattttggaaaacaacatggtgcagacccaagcctcggctgcgggtacgctttcgaccatcatcttcgtcctgcccggtttgctgatggcgggctactggagcggtttcccgttctggcagacgacgcttttatgtattgccggcgggattttgggggtgattttcaccattcctctgcgttacgcaatggtggtgaaaagcgatttgccttatccggaaggtgtggcggctgctgaaattttgaaagtgggcggtcatgaagaaggggataaccgtcagggcggcagcggcatcaaagagctggcggccggcggtgcgttggcgggattgatgagcttttgcgccggaggtctgcgcgtgattgccgacagcgcgagttattggtttaaaagcggtacggcgattttccagctgccgatgggcttttcactggcattgttgggcgcgggctatttggtcggactgacgggcggtatcgccatcctgttgggcatttcgattgcttggggcattgccgtgccgtatttctcctcacacattccgcaaccttccgatatggaaatggcggcgtttgcgatgaagctgtggaaggagaaagtgcgttttatcggtgcggggactattggcattgcggcggtttggacgctgttgatgctgctcaagccgatggtggaaggcatgaagatgtcgttcaagagttttggcggcggtgcgcccgctgcggaacgcgccgaacaggatttgtcgcctaaggctatgattttttgggtgctggcgatgatgtttgttttaggcgtgtcgttttaccactttatcggcgattcgcacattacgggcggcatggcttggcttttggtggtcgtttgcacgcttttggcttccgtcatcggctttttggtcgccgccgcctgcggttatatggcaggtttggtcggctcgtcttccagcccgatttccggcgtgggcatcgtgtccgtcgtcgttatttcactggttttgctgctggtaggcgaatccggaggtttgttggcggatgaggctaaccgcaaatttttgctggcactgactttgttttgcggctcggcagtaatctgcgtggcttcgatttccaatgacaacctgcaagacttgaaaaccggctacctgctcaaagccacgccttggcggcagcaagtcgccctgattatcggctgtatcgttggtgcgctggttatttcgcccgtgttggaactgctttacgaagcctacggctttaccggcgcaatgccgcgcgaaggcatggacgcggcgcaggctttggcagcccctcaagcgactttgatgacgaccatcgcgtcgggcattttcgcccacaaccttgaatgggtctatatctttaccggtatcgtgattggagcagtattaatcgtcgtcgatttggtgttgaaaaaatcatcaggcggcaaacttgccctgcccgtccttgcggtcggtatgggtatttatctgccgccgtccgtcaatatgcccatcgtggcaggcgcggtgttggcggcggtgttgaaacacatcatcggtaaaaaagcggaaaaccgcgaaggccgtctgaaaaacgccgagcgcatcggaaccttgttctccgccggcctgattgtcggtgaaagcctgatcggtgtgattatggcgtttattattgccttctccgtgaccaacggcggctcggatgcgccgctcgcgttgaatctgcaaaactgggatgccgccgcttcttggctgggtttggcgttcttcgttaccgggatgtttttctttgcacagcgcgtactgaaggcgggcaagtaggctgtcggaaaaaatgccgtctgaaacgttcagacggcattttttatcggtaaagcggaaggcggagcttttcggcttgcgcccacgttttgccggcaaggtctttgggcgacagcagcggcgcggtttgaagcggccagcctatgccgactgtcgggtcgttccatattaaaacctgttcggcttcaggcttgtaatagtccgtgcatttatagacgaactcggcttcatcgctcagtacatagaagccgtgtgcgaaaccttcgggtacccacagttggcgtttgttttctgcggacagaatttcgcctacccatttgccgaaagtgggggagtctttacgcatatcgacggccacgtcgaatacttcgccgacaaccacgcgtacgagtttgccttgtgtgttttcagtttgatagtgcaggccgcgcaatacgcctttgccggatttggagtggttttcctgcacgaaggtgcgttcgcagacttgggttttaaaccactcgtcgcggaaggtttccataaaaaagccgcgcgcgtcgccgaagacttggggctcaagcagttttacgtcaggaatggcggtatcaatgatgttcatctttttatctttcatctaaaggccgtctgaaaagtttcagacggcctcaaacattattttttcaacaggcgcagcaaatattggccgtattggtttttcgccatcgggcgcgccaattcttccagtttttcatcggaaagccaaccgttgcgccaagcgatttcttcgaggcaggcgatgtgcaggttttggatattttgcacggtttggacgaatgaagcggcttcgtgcaggctctcgtgggtgccggtgtccagccacgcgaaaccgcgtcccaatatttgaacggagagcgagccgtcttccaaatacatccggttgaggtcggtaatttccaattcgccgcgtgcggacggtttgagctgtttggcgaactcgacggcgcggttgtcgtagaaatacaagccggttaccgcccaatcggatttgggccgttgcggtttttcttcgatggaaacggcgcggaagttttcgttaaattcaaccacgccgaaacgttcggggtttttgacctgataagcaaacacggttgcgccgtgcgtttgcgctgccgcctgtttcaatgtttgcgtaaacgactgaccgtaaaaaatattgtcgcccaaaaccaagcaaacattgtcgttgccgataaattcttcgccgatgataaatgcctgtgccaagccgtccggactgggttgcacggcataactgatggaaatgccgaaatcgctgccgtcgccaagcaggcgtttgaaagaggcgttgtcttcaggcgcggtaatcaccaaaatatcgcggattcccgccagcatcaaaaccgacaaggggtaataaatcatcggtttgtcgtacacgggcaggagctgtttggatacgccgcgcgtgatggggtagaggcgcgtgccgctgccgcctgccagtatgatgcctttcatcttttctttcttcctttgcgatgggttttcagacggcattgcgtcgggatgccgtctgaaaactattttccagtacctaaacgttccaaacgatagctgccgttcaatacattttgccaccaggttttgttgtccagataccattgcacggttttgcggaggccggactcgaaggtttccaaaggcagccagcccaaatcccgcctgattttggctgcgtcgacggcgtagcgtacgtcatggccggggcggtcttgtacgaaagtaatcaaatcttcataacgcgccacaccggccggtttttcgggagcgagttcttccagcagggcgcagatggttttgacgacttcaatattggctttttcattgtggccgccgatattgtaggtttcgccgacaacaccttcggtaacaacctgatacagtgcgcgcgcgtggtcttcgacaaacagccagtcgcggatttgcataccgtcgccgtacacaggcagcggtttgccgtcaagcgcgttcagaatcatcaaaggaatgagtttttccggaaaatggtaaggaccgtagttgttggagcagttggttacaatggtcggcaagccgtaagtacgcaaccacgcgcggacgaggtggtcgctggacgctttagaggcagagtaggggctggacggcgcgtagggcgcggtttcggtaaacaaatcgtccgtgccgcctaaat

附录Bcgccatagacttcatcggtggaaatatggtggaaacggaaggcttcgtgctgttcagacggcatttgttgccagtaggcgcgggctgcttcaagcagattgaatgtgccgacgatattggtttggataaactcgcctgccgaaccgatagagcggtcgacatggctttccgccgccaagtgcatcacggcatcaggccggtattgcgcgaatacgcggtcgagttcggcgcggtcgcaaatatccacttgttcaaaagcatagcgaggattatcggctacctcagtcaaagattccaaattgccggcataagtcagcttatcgacattgacgacagcgtcccgggtgtttcggataatatgacggacaacggcagaaccgataaagcccgcgccgccggtaacaaggatttttctcataaatttcagaggatagccaaaaaatataaacagattatagcagacagaatgtgtgtttttcagataaagaggccgtctgaaaacatctctttcagacggcctgtatcaggtcaacttaatcgtcgtagccattcggattattactcacccagcgccatgagtcttccatcatttgggttaaatcacgctgggtttgccagccgatttgcgcctttgtataggaagggtcggcatagaagcacgccaaatcaccggcacggcgcggtttgacttcatacggaatcgtcaaacccgaagctgcttcaaatgcgcggatgatttccaacaccgaagaagcgcggccggagcctaagttcagcaaatgcgtgcctgctacattactttttgcctgcatagccgcgacatggccttctgccaaatccatcacatgaatatagtcacgcatccccgtgccgtcgggggtagggtagtcatcgccaaataccgccaattgcggcagtttgcctgccgccacttggcagatataaggcaacaaattattcgggatgccgtttggctgctcgccaatcaagccgctttcatgcgcgccaatcggattgaaataacgcaacaaaatcatgctccagcgcggatcggctttttgaatgtcagtgagaatgcgctcaaccatcgatttcgatgcgccgtaagggctggtggtgtcgcccggtggcatatcctcggtataaggcactttgcccggatcgccataaaccgtcgccgaagaactgaacacaatgctaaacacgcccgcacgcgccatttcttccgccaacaccaagctgccggaaacattattatcataatatttcatcggctcggccacactttcacccaccgctttcaagccggcaaaatgaatcaccgaatcaatgcggttttccgcaaaaatacggcgcaaaatctcacgatcgcggatatcgccttgataaaacggaatctcttggccggtaatcgttttcaagcgtggcaggatattgatgctggaattgcataggttatccaaaatcacgacttgatggccgcttttcagcaaagaaacaacggtatgcgagccgataaaaccggtgccgccggtaacgagaatttttttcatagaataaaatactaaaaatactttgatagattgataataatggttgtaaaatcttaatgaaataattatccctgaagtagcagtagatttcttcagatttttttggttaagtatatttgatatctaaggtaaaatactataattttattcatatggtgtagaattaagggaaaatagtgaaaaaagtattactaattgccagttatgactcgttccttaactcgggctatgctgttgcaaaagagataaaagatgctcaaattgatatttatatccacaaaagtcgagaaaacattctttcaaatcgacagttattagaatcagggatagataaagaccaagcaattttttttttcatattgatgattactttattaagaatatgcatcaatattatgacgcagtaattttatcggttggaaatgggttgttaaaaaggttctttaagcagaatgcgcaattaaatattgcttcaaggccattgattattaccttgtttccaggtgtagtattcggtgatcaggcaagtattctatctcgtatgggggctgatattgttttatataataataagcatgattttagaattgcagaggaatataagaaacaatataaattaagttgtcaaaatatactttatggttatccaatttttcgccatgcttcgaaaggttgtcatggagagaaaatttactttattgaccaagttaaaatcccatttaaaaaagaagaaagaatttatacattaaaaaaattgattgccttggctgaaaaataccctgagaaagaatttactattttgctaagggttgcagataaagatattactgtgcatcaggataaacattcgtatatagagctggcaaagcagtttcagttgccgagtaatttgacaatagagcgaaaaagtaccgcgcaagccttccaagaaatggggtattgtttatcttattcatctactatgctttttgaagctgaatgtaagggtatccctgttggtgttgttgcagacttaggcttttctaaatcctatgcaaatcagcattttttaggtagtggggttttagtttattttgatcaaatagatttcacttccccaaaaatagcagatccggattggcttgattgctatgctactaaaaaggtgattacaactgatgagtttaataagctattaaagcaggttgtgccattgcaacatgattaccaagaatatttatctgcaggaattcgatatcaagctttggctaacacacacgccattccaaccaatagttttctcggcataaagccatgctctgacgcttaaatgcactaatgccttaaaaaaacattaaagtctaacacactagacttatttacttcgtaattaagtcgttaaaccgtgtgctctacgaccaaaagtataaaacctttaagaactttcttttttcttgtaaaaaaagaaactagataaatctctcatatcttttattcaataatcgcatcagattgcagtataaatttaacgatcactcatcatgttcatatttatcagagctcgtgctataattatactaattttataaggaggaaaaaataaagagggttataatgaacgagaaaaatataaaacacagtcaaaactttattacttcaaaacataatatagataaaataatgacaaatataagattaaatgaacatgataatatctttgaaatcggctcaggaaaagggcattttacccttgaattagtacagaggtgtaatttcgtaactgccattgaaatagaccataaattatgcaaaactacagaaaataaacttgttgatcacgataatttccaagttttaaacaaggatatattgcagtttaaatttcctaaaaaccaatcctataaaatatttggtaatataccttataacataagtacggatataatacgcaaaattgtttttgatagtatagctgatgagatttatttaatcgtggaatacgggtttgctaaaagattattaaatacaaaacgctcattggcattatttttaatggcagaagttgatatttctatattaagtatggttccaagagaatattttcatcctaaacctaaagtgaatagctcacttatcagattaaatagaaaaaaatcaagaatatcacacaaagataaacagaagtataattatttcgttatgaaatgggttaacaaagaatacaagaaaatatttacaaaaaatcaatttaacaattccttaaaacatgcaggaattgacgatttaaacaatattagctttgaacaattcttatctcttttcaatagctataaattatttaataagtaagttaagggatgcataaactgcatcccttaacttgtttttcgtgtacctattttttgtgaatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccaggcaaaaaaccggttatatttttttgcattaaatatttttttagcatattcaggaaaggggacatgcaatatgtcaaaatgatctatatatcctttaatattaagattatttccaatcaaataacgttctaattttgttggatgatatgaaaatgattctaataaaggagcatatgttccagtcccttcatcaattaaatgagtcgtaatattctttttttttgcaatactaatcagataggagtagtggcctgtaaaagacagcatatagagatgagcaggctgtataatattaaggatttttttgtaacttctataaatataaagtaattttttaggagttatattattagggcttctaggaagctcaaatagataaatagattcaaatagattcttgttagctgattgatgaactaacttaggcatttttaagtttttagaagtatataaaattactagtaaattattggttaatttttgtattttaattaggctttggacttggttaagctgacctaaattagatatgacaaataaattgttacgtgggggggtaagataaaatggagatgttgtcaaccacattgaatcttgaaaaaactttttaggctgaaaaagagctttttttattttctttagcattattgtatctcttaaaaattaatgagaattagctatatgtaatagccaatcctctgttaataaagtaactaagttaataagcattattcaatatcagtttttttgatttgagcacctttgcgaatattgcaagcagcgaccttaccaaataatgtttcatattcgttgacgctgaagtctccattgcctgggcgtttaacccataggttatctccggacaacagttctccttttttaatgtctttatctgctacgacagatgcaaaggcgaaatctttagttggcttttctcccgcgataatcgtgtcttttttgccgccgcgtgccaattttaaagcatgagcgccttgcttgagctctttaaaagtatccggattcatagagcatacaatatccggacctgggcgatccatgcggtcagtaaagtgacgctctaaaatcgaaccgcctaaagctactgctcctaagcaagcatagttatctaaggtatggtcagacaggccaatgattgcgtctggaaaggcttcagataaatcgttcataccacccaatcgaacatcttcgtaaggggttgggtagatgttggtacagtgaagcaaagcataaggtacccctgcttctcgaataatttctaccgactttttgatgctttcaatagaattcatgccggtagagagaataataggcttaccaaaagaggccaccagtttaattaatgggtagttattacattcgccagagccgattttatatgctggaatatccatacgttgtaatcgtaaagcagctgcacgagagaaaggagtactgataaaaatcatacccttactctctacgtattcttttaatttaatctcatcttcttcattcagggcgcaacgttccataatttcataaatagagacatctgcattgcctggaatgacttgtttggcctcatcagacatttcgtcttcaacgatgtgtgtttgatgtttaacaacttcagcgcctgcattataggcagcatcaaccatttcaaaagctgtttttaaagagccttcatgattgatgccgatttcacagataatcaatggttcgtggttgtaacctactgaacgattaccaattttaaattcgttgttgttttgcatttagctttccttgtgattaagaatgttttctgcctgttgtaaatcaagctcagtatcaatatcgatagagtcttgatgagacataatataaagtttggttggggcgataaaaaaacaattatttgcaattagtgaagcagtatcattaatgtaaattgcaccattaggcctaaatgcctgaggtaattgttggcgaggctgctccaaatcgcttagatggcgcatgggggcatattcgccattattgatttgaagcagggtttttagtggatgatgctccattgggcatgcagagacaacggatccttttattttctcatcaaatagagaaaaagcttcacgaatatgagcccctgtgcgtaatggactggttggttgtaatagggttactgtgccggaattactgccaattgtttctaaagcatgtattacacctgaaatagagctggctgtatcggaggccagctctgcagggcgtaggacgacttcgacaccgaaatttttagcttcttctgcaattaacccgccatcagtcgaaacaattatgcggtcaaaacactttgatgatatagcagcattaattgtatgaccaagtaatgatatgccattcattttccggagattttttaatggcaatcctttggagttttggcgcgcaagtataaccgcaatattttgtttttccataatttaaagattcaaatcgataaaacgtttttgagcagaaacattccacgtttcaggattgttgattacttcagcaaatctttctgtgctggtgcga

附录Bgtatctccgccattaaaggtatcatctgcttcaaatttgcctaaactgcatgcttgttgaatcgcatcaaagatatttttagtttcataatctgtatgaataatagattttcccatatggcggttactttggcgtgtaccaacatcaattgaagggacaccgtagagaggagcttctctaatacctgcacttgagttgccgaccataaatttagcatgtttcaataagactaaaaaatattcaaatcgaatggaaggaaatgcaataaatttatcagattgatattttaataattcttgcagaatactttcagtgccagtgtcattattagggtagatgctaatgatattttggccacttaattctaatgctttgaaatattgggccgcatattgtggcattaaatgtgcttctgtagtcacggggtgaaacatagaaataccataattttcgtatggtaaaccgtaatattctttgacttcttctaaggatgggagggtggaagaggccataacatctaaatcgggggagccgatgatgtgaatatgctttcttttttctcccatttgcactaggcgagtgacagcttgttcatttgctaccaagtggatatgagaaagtttactaatagaatgacgaatggagtcatctactgtaccagatagttcaccaccttcgatatggcaaactaaacggctgcttaatgcacctacagctgcgcctgctagtgcttctaaacggtcgccgtgaatcatgaccatatcaggttcaatttcatcagatagacgagagataaacgtaatggtattgcctaaaacggcacccattggttcaccttggatttgatttgaaaacagatatgtatgttgatagttttctcgagttacttccttgtaggttctgccatatgttttcatcatatgcataccagttacaatcaaatgcaattcaaggtctgggtgattttcaatataggctaataaaggttttagcttgccgaagtcggctctggtacctgtaatgcaaagaattcttttcatgattttagaatctataagtatataagtataaggaagttggaaagaagaatactaattatactctacgtactcataaatttatttcgattaagtgctataattaggccatttataattatattaggatttggcttgtgtttaaagtgaaattttatattcgtcacgcagtattattattgtgtggaagtttaattgtaggatgctctgcgattccttcatcaggccccagcgcaaaaaaaattgtctctttagggcaacaatctgaagttcaaattcctgaagtggagctgattgatgtgaatcatacggttgctcagttattatataaggctcagataaatcagtcattcactcagtttggcgatggttatgcttcggctggtacgctaaatattggtgatgtattggatattatgatttgggaagcgccgccggcagtattgtttggtggtggcctttcttcgatgggctcgggtagtgcgcatcaaactaagttgccagagcagttggtcacggcacgtggtacggtttctgtgccgtttgttggcgatatttcggtggtcggtaaaacgcctggtcaggttcaggaaattattaaaggccgcctgaaaaaaatggccaatcagccacaagtgatggtgcgtttggtgcagaataatgcggcgaatgtgtcggtgattcgtgctgggaatagtgtgcgtatgccgctgacggcagccggtgagcgtgtgttggatgcggtggctgcggtaggtggttcaacggcaaatgtgcaggatacgaatgtgcagctgacacgtggcaatgtagtacgaactgttgccttggaagatttagttgcaaatccgcgacaaaatattttgctgcgtcgcggtgatgtggttaccatgattaccaatccctatacctttacgtctatgggtgcggtggggagaacacaagaaatcggtttttcagccagaggcttatcgctttctgaagccattggccgtatgggcggtttgcaagatcgccgttctgatgcgcgtggtgtgtttgtgttccgctatacgccattggtggaattgccggcagaacgtcaggataaatggattgctcaaggttatggcagtgaggcagagattccaacggtatatcgtgtgaatatggctgatgcgcattcgctattttctatgcagcgctttcctgtgaagaataaagatgtattgtatgtgtcgaatgcgccgttggctgaagtgcagaaatttttgtcgtttgtgttctcgccggttaccagtggcgcgaacagtattaataatttaactaattaatgtgagtaattaagatgtctgagcaacttcctgtggcagttgccactgaaaccaaagccgagcgtaaaaagccgaaaaagaaaagttggattaaaaagctaagccctttattttgggtaacggtgattatccctacggtaatttcgttggtgtatttcggcttcttcgcttccgatcgttttacgtcgcaatcgagctttgtggtgcgctcgcctaaaagccaatcttctctcaatggcctgggtgccattttgcagggcacaggttttgcccgtgcgcaagatgatatttacacggttggggagtatatgcgttcgcgctcgtctttggatgaactgcgtaaaatcttgccggtgcgtgagttttatgaaaccaaaggtgatgcgttcagccgctttaatgggtttgggttccgtggcgaggaagaggctttttatcaatactataaaaatcaggtgatgatcaattttgatacggtttcgggtatttccacgttgaatgtaacttcctttgatgcgctggaatctaagaaaatcaatgaggctttgttaaaacaaggtgaagcattgattaaccagttgaacgatcgtgcacgtgctgatacggtgcgctatgcggaagaagtagtgaaaacggcggcagagcgggtaaaggaagcctctcagaatctgacggattaccggattgccaatggcgtttttgatttgaaagcgcaatcggaagtgcaaatggggttggtttccaagctgcaagatgaattgattgtgattcaaacccagctggatcaggtgaaagcagtcactccggagaatccgcagattccgggtttgcaggcgcgtgagcagagcttgcgtaaagaaattgaccaacagttacgtgccatttcgggcggtgggcattcttcgttgtctaatcaggctgccgaatatcagcgtgtgtatttggaaaaccagttggcagagcagcagttggcagccgccatgacttctttggaaagtgccaaggttgaagcagaccgtcagcagctttatttggaagtgatctcgcaaccgagcctgccggatttggcacatgagcctaaacggttatacaacattgttgccactctgattatcggcttgatggtttatggtattttgagcctgttgactgccagcattcgtgagcataaaaactgatgaaagccttgcataaaacatcattttgggaatctttagccattcaaaggcgcgtaatcggtgcgctgttgatgcgggaaattatcacccgttacggtcgcaataatattggctttttatggctgtttgttgagccgttgctgatgacattcgttatcgtcttgatgtggaaatttttaagggcagaccgatattcaactttgaatattgtcgcatttgcgattactggctatccgatgttgatgatgtggcgtaatgcctcaaaacgggcagttgggtcgatttcttcaaatgccagcttgctttatcaccgcaatgtaagagttttggataccatcttggcgcgcatgattttggaaattgctggtgcaaccattgcgcagattgtgattatggcggtattgattgcgattggctggattgaaatgccggcagatatgttttatatgctgatggcttggcttttgatggctttttttgcgattggtttgggtttggtgatttgttcgattgcctttaatttcgagccgtttggcaagatttggggcacattgacttttgtgatgatgccgttatccggtgcgttcttttttgtgcataatttgccgcccaaggtacaagaatatgcattaatgattccgatggtgcatggcacagaaatgttccgtgccggatattttggcagcgatgtaattacctatgaaaatccttggtatatcgtattgtgcaatctggtgttgttgttgtttggcttggcgatggtcagtaaattcagtaaaggagtcgagccgcaatgatttcagttgaacacgtttccaaacgctatctgacccgccaaggttggcggacagtcttgcacgatattagcttcaaaatggagaagggcgagaaaatcggtattctcggccgcaacggtgcaggtaaatcgacgctcatccgtttgatcagtggcgttgagccgccgaccacgggtgaaatcaagcggacaatgagtatttcttggcctttggcattctccggtgcgtttcaaggcagtctgaccggtatggacaatttgcgtttcatctgccggatttacaatgtcgatatcgattatgtgaaagcgtttacggaagaattttcggagctggggcaatatttgtatgagccggtgaaacgctattcttcaggtatgaaagcgcgtttggcttttgcgctgtcgttggcggtggagtttgactgttacctgattgacgaagtgattgcagttggtgactcgcgttttgccgataaatgtaagtacgagttgtttgaaaagcgcaaagaccgttccatcatcttggtgtcgcacagccacagcgccatgaagcaatattgcgataatgcgatggtgctggaaaaagggcatatgtaccagtttgaagatatggacaaagcctacgaatattataattcgctgccttaaagcgattgtttttaaatcaggccgtctgaaatttcagacggcctgtccgttggaattctattgatgaacattactcaaattctttcccaagaactctccgcgactgccgcgcaaatcaccgccgccgtcgagcttttggacgacggcgcgaccgtgccgtttatcgcccgctaccgcaaggaagcgacgggcgggttggacgatacgcagttgcgccggcttgccgagcggctgcaatatctgcgcgagttggaagagcgcaaagccgttgttttaaaaagcattgaagagcaaggcaagctttcagacgacctcagggcgcaaatcgaagccgccgataacaaaaccgcgctggaagacctgtatctgccctacaaacccaaacgccgcaccaaagcgcaaatcgcgcgcgaacacggtttgcagccgctggcggacgtgttgcttgccgagcagtcgcaggacgtggaagccgccgcacaaggctacctgaacgaaaacgtccccgatgccaaagccgcgttggacggcgcgcgtgcgattctgatggagcagtttgccgaagacgcggaacttatcggcacgctgcgcgacaagctgtggaacgaagccgaaatccacgcgcaagtcgttgaaggcaaagaaaccgaaggcgaaaaattcagcgattatttcgaccaccgcgaacccgtccgcactatgcccagccaccgcgcgctggcggttttgcgcggccgcaacgaaggcgtgttgaacatcgcgctcaaataccagcccgacgacacgccgattacccggcaaagcgaatacgagcaaatcatcgcctgccgcttcaaggtttcagacggccacaaatggctgcgcgataccgtgcgtctgacttggcgcgcgaaaatctttttgtcgttggaacttgaagccctaggccgtctgaaagaagccgccgacaccgacgcgattaccgtgttcgcccgcaatctcaaagacttgctgctcgtcgcgcccgccggacggctgaccacgctgggtctcgaccccggctaccgcaacggcgtgaaatgcgccgtggtggacgacaccggcaagctgctggataccgtcatcgtctatttgcatcaagaaaacaatatgttggcaacgctgtcgcgcctgattaagcaacacggcgtgaagctcatcgccatcggcaacggcaccgccagccgcgaaaccgacaaaatcgcgggcgaactggtgcgcggaatgccggaaatggggctgcacaaaatcgtcgtgtccgaagccggcgcgtcgatttattccgcgtccgaactggcggcgcgcgagttccccgacttggacgtttccctgcgcggcgcggtgtccatcgcccgcaggctgcaagacccgcttgccgagttggtcaaaatcgaccctaaatccatcggcgtgggccagtatcagcacgatgtgaaccaaaaccagctcgccaaatcgctggacgcagtggtcgaagactgcgtgaacgccgtcggcgtggacgtgaataccgcctccgccccgctcttggcgcggatttccggcttgaatcaaacccttgcccaaaacatcgttgcctaccgcgatgaaaacggcgcgttcgacagccgcaaaaaattgctgaaagtaccgcgtttgggcgaaaaaaccttcgagcaggcggcaggctt

附录Btttgcggattaacggcggtaaagagccgttggacgcgagcgccgtccaccccgaagcctatcccgtcgtcgccaaaatgctggcgcaacaaggcattagcgccgccgaactcatcggcaaccgcgagcgcgtgaagcaaatcaaagcgtccgacttcaccgacgaacgcttcggcctgccgaccattttggacatcctgtccgaactggaaaaacccggccgtgatccgcgcggcgagtttcagacggcatcgtttgccgaaggtatccacgaaatcagcgacttgcaagtcggtatgatactcgaaggcgtggtttccaacgtcgccaacttcggcgcgttcgtggacatcggcgtccatcaggacggcttggtgcacatctccgccctgtccaacaagttcgtccaagacccgcgcgaagtggtgaaagctggcgacgtggtgaaagtgaaagtgctggaagtcgatgctgcacgcaaacgcatcgcgctgaccatgcgcttggatgacgaaccgggcggcgcaaaacataaaatgccgtctgaaaaccgcagccgcgaacggacagccggccgcaaaccccaacgcaacgaccgcgccccagccaattcggcgatggcggatgcgtttgcgaagctgaagcggtaaaataatcgaagagtttatggattttgacttatgcacacaccacttacctatattgaccttttctcaggagcaggaggcctatccttgggttttgaacaagccggattccaacaattgctttctgttgaaatggagtctgattattgtcagacttaccgtaccaacttcccccatcatcaattactgcaaaaagatttaaccacactaaccgaacaagatttaatcaattgtcttaacggacaagcagttgatttgattattggaggaccaccttgtcaaggttttagtatggcaggaaagattggacggacatttacagatgacccacgcaaccatttatttaaagagtttgtccgaatagttaaaattgtccaaccatatttttttgttatggaaaatgtagcgcgactctatacacacaattcaggtaaaacacgtattgagattattcaagcatttcagaatatcggttattcggtggaatgtaagatactgagtgcagccgatttcggtgttcctcagatacgtagccgagtgatatttatcgggaggagggataaaggcaaaatttcctttcccgaacctttgcagatttcccatcagactgttggatcagcaataggacattttccaaaactggctgctggcgaaagcaatccacacgttgcaaatcatgaagctatgaatcattcggcacaaatgttagaaaaaatggcatttgttaaaaatggaggtaaccgtaacgatattcctgaaccattacgtccgaaaacaggtgatatccgtaaatacatccgttacaacagcaacaaaaccagccgtttgtattacaggagatatgcgcaaagtttttcactatgaacagaatcgggcgttaaccgttcgtgaattagctgccttacaatctttccctgataattttattttttgcggcagcaaaattgcccagcagcagcaggttggtaacgccgtaccgcctttattggcaaaagctattgctgaaagtattttaaaaatgagtgaaaatgaataagcaatatccgaaaattaactatatcggtaataaagagaaaatagcttcctggatttgtgaccagcttccgtctgatgtagatacagttgcagatgtatttagtggaggctgttcctttgcctacgaagccaaaaaacgcggctatcgtgtgattactaacgatattttggcaattaattaccaaattgctttagcattaatagaaaacaaccatgaaacattaaatgacgatgatgtcgcaatgattttttcaggcagcccgcatgccggttttatgagtcagcgttatgccgaaaaattctattttcacgatgaataccaacaacttgatttgtaacgtaaaaatatagggaaactggataaccagtataaacgcgctttggcgtttactttaatgcgtcgcgccatgatacgtaaaatgccctatacggaagatatgcgcccaggcgataccgctaatccttatggtgcgtccaaagcgatggtggaacggatgttaaccgacatccaaaaagccgatccgcgctggagcatgattttgttgcgttatttcaatccgattggcgcgcatgaaagcggcttgattggcgagcagccaaacggcatcccgaataatttgttgccttatatctgccaagtggcggcaggcaaactgccgcaattggcggtatttggcgatgactaccctacccccgacggcacggggatgcgtgactatattcatgtgatggatttggcagaaggccatgtcgcggctatgcaggcaaaaagtaatgtagcaggcacgcatttgctgaacttaggctccggccgcgcttcttcggtgttggaaatcatccgcgcatttgaagcagcttcgggtttgacgattccgtatgaagtcaaaccgcgccgtgccggtgatttggcgtgcttctatgccgacccttcctatacaaaggcgcaaatcggctggcaaacccagcgtgatttaacccaaatgatggaagactcatggcgctgggtgagtaataatccgaatggctacgacgattaagttgacctgatacaggccgtctgaaagagatgttttcagacggcctctttatctgaaaaacacacattctgtctgctataatctgtttatattttttggctatcctctgaaatttatgagaaaaatccttgttaccggcggcgcgggctttatcggttctgccgttgtccgtcatattatccgaaacacccgggacgctgtcgtcaatgtcgataagctgacttatgccggcaatttggaatctttgactgaggtagccgataatcctcgctatgcttttgaacaagtggatatttgcgaccgcgccgaactcgaccgcgtattcgcgcaataccggcctgatgccgtgatgcacttggcggcggaaagccatgtcgaccgctctatcggttcggcaggcgagtttatccaaaccaatatcgtcggcacattcaatctgcttgaagcagcccgcgcctactggcaacaaatgccgtctgaacagcacgaagccttccgtttccaccatatttccaccgatgaagtctatggcgatttaggcggcacggacgatttgtttaccgaaaccgcgccctacgcgccgtccagcccctactctgcctctaaagcgtccagcgaccacctcgtccgcgcgtggttgcgtacttacggcttgccgaccattgtaaccaactgctccaacaactacggtccttaccattttccggaaaaactcattcctttgatgattctgaacgcgcttgacggcaaaccgctgcctgtgtacggcgacggtatgcaaatccgcgactggctgtttgtcgaagaccacgcgcgcgcactgtatcaggttgttaccgaaggtgttgtcggcgaaacctacaatatcggcggccacaatgaaaaagccaatattgaagtcgtcaaaaccatctgcgccctgctggaagaactcgctcccgaaaaaccggccggtgtggcgcgttatgaagatttgattactttcgtacaagaccgccccggccatgacgtacgctacgccgtcgacgcagccaaaatcaggcgggatttgggctggctgcctttggaaaccttcgagtccggcctccgcaaaaccgtgcaatggtatctggacaacaaaacctggtggcaaaatgtattgaacggcagctatcgtttggaacgtttaggtactggaaaatagttttcagacggcatcccgacgcaatgccgtctgaaaacccatcgcaaaggaagaaagaaaagatgaaaggcatcatactggcaggcggcagcggcacgcgcctctaccccatcacgcgcggcgtatccaaacagctcctgcccgtgtacgacaaaccgatgatttattaccccttgtcggttttgatgctggcgggaatccgcgatattttggtgattaccgcgcctgaagacaacgcctctttcaaacgcctgcttggcgacggcagcgatttcggcatttccatcagttatgccgtgcaacccagtccggacggcttggcacaggcatttatcatcggcgaagaatttatcggcaacgacaatgtttgcttggttttgggcgacaatattttttacggtcagtcgtttacgcaaacattgaaacaggcggcagcgcaaacgcacggcgcaaccgtgtttgcttatcaggtcaaaaaccccgaacgtttcggcgtggttgaatttaacgaaaacttccgcgccgtttccatcgaagaaaaaccgcaacggcccaaatccgattgggcggtaaccggcttgtatttctacgacaaccgcgccgtcgagttcgccaaacagctcaaaccgtccgcacgcggcgaattggaaattaccgacctcaaccggatgtatttggaagacggctcgctctccgttcaaatattgggacgcggtttcgcgtggctggacaccggcacccacgagagcctgcacgaagccgcttcattcgtccaaaccgtgcaaaatatccaaaacctgcacatcgcctgcctcgaagaaatcgcttggcgcaacggttggctttccgatgaaaaactggaagaattggcgcgcccgatggcgaaaaaccaatacggccaatatttgctgcgcctgttgaaaaaataatgtttgaggccgtctgaaacttttcagacggcctttagatgaaagataaaaagatgaacatcattgataccgccattcctgacgtaaaactgcttgagccccaagtcttcggcgacgcgcgcggcttttttatggaaaccttccgcgacgagtggtttaaaacccaagtctgcgaacgcaccttcgtgcaggaaaaccactccaaatccggcaaaggcgtattgcgcggcctgcactatcaaactgaaaacacacaaggcaaactcgtacgcgtggttgtcggcgaagtattcgacgtggccgtcgatatgcgtaaagactcccccactttcggcaaatgggtaggcgaaattctgtccgcagaaaacaaacgccaactgtgggtacccgaaggtttcgcacacggcttctatgtactgagcgatgaagccgagttcgtctataaatgcacagactattacaaccccaaagccgaacactcgctgatttggaatgatccgaccgtcggcatcgactggccgttgcaaggcgagccgaacctgtcgcctaaagacttggcaggcaaagtattgtctgaagcggtaacgttttaaaaataattcaggccgtctgaaagaatgttcctcttttcagacggcctacaatccattaataacaataatcgacgaaaacgcattgtgaaaaacgcctacatcccctctcgcggcatccgcaaaatcccccatctctccaccctattgcctgaatttcatatctgcaaagacgggaaagaagcagaggctgttgtcggctggggtttgcgcccgacgacacacaaagcgcgtgcttttgccgctgaacaccagcttccctttattgctttggaagacggctttttacgatcgctcggactgggtgtcgccggttatccgccctactctatcgtctatgacgacatcggcatctactacgacaccacacgtccttcgcgtttggaacaactgattcttgccgccgataccatgccgtctgaaaccttggctcaggcgcagcaggcgatggatttcatcctgcaacaccacctgtccaaatacaaccacgcgcccgaactttcagacgaccatcctttacgttccccatccaaacccgaaaccgtcctcatcatcgaccaaaccttcggcgatatggccatccaatatggcggcgcagacgcctctacgtttgaactgatgtttcagacggccttaaatgaaaacccgcaagccgatatctgggtaaaaacccatcccgatgttttgtgcggcaaaaaacaaggctatctgacccaactggcgcagcaacaccgcgtccatcttttggcagaagacatcaatccgatttctttgttgcaaaacgttgataaagtttattgcgttacctcgcaaatgggttttgaggcgcttttgtgcggcaaaccgctgaccactttcggcctgccgtggtatgccggatggggtgtaagcgacgaccgccatcctgaaatcaaccgccttgttcaaacccaacgccgcgccacccgcaacttgctgcagctcttcgccgcagcctatctgcaatacagccgctacctcaaccccaataccggcgaagcaggcagcctctttgatgtcatcgactatctggcgacggtcaaacgtaaaaacgacaaattgcgtggcgagttatattgcgtcggtatgtctttgtggaaacgcgcggttgccaaaccgttctttaacgtaccctcttgccgtctgaaatttatctcttccacccaaaaactggcaagggtcaaactgtccgacgatgcacgcatcctggcttggggcaacg

附录Bgcaaagaggccatcgtccgctttgccgaacaacaccacatccccctgctgcgcatggaagacggctttatccgctcggtcggactcggctccaacttagtgccgccgctgtcgctcgttaccgacgatatgagcatttatttcaatgccgaaaccccgtcccgtcttgaatacatcctacaaaaccaaaacttcgacgatcaagactttcagacggccttgaagctgcaaaaaatgctgaccgaaaaccacatcagtaaatacaacgtcggcagctcagacttcaccgccccgtcaaccgacaaaaccgtgatcctcgttcccggccaggttgaagatgatgcgtctatccgctacggttcgccccaaatctaccgcaatctggatttgctccgtaccgtacgcgaacgaaaccccaatgcctatatcatctacaaaccgcatcccgatgtagtcagcggtaaccgcatcggccatatttcccctgaagatgctgcacgatatgccgaccaaaccgccgaacaagccgacatcctgacctgtctccaatacgcagacgaaatacataccatgacttcgctgaccggttttgaagccttgttgcgcggcaaaaaagtcagctgctacggcctgcctttttacgcaggctgggggcttacccaagatctgctccccatcccgcgccgtagccgcagacttgagctttggcagctgattgccggcacgctcatccactatcccgactacatccaccccgaaacccatcaggccataaatgcagaaaccgcagcccaaatcctgatacgacaaaaaaatatgcaaaaaaacaacaacggattacatcgcgggtgctttgccaaaaaattaggtaaaatcaaacaactatatcgatctttcaaataaataccatcaaagttaacgatgcgtcataaacttgcctctattgcggcatcattgcctttgcatcgttaattctcttggcgtatgcttgaaagttcaacctaaaactattacataaaaaacaaaaccacattgcaacatgaaacagaccgtcctcaaaaataacctgcaaaacctgcttgaaagcgcagaaaatatcctgctgcttcaaggccctgtcggcgatttttttctgcgccttgccgactggctgactgcaaacggcaaaaccgtacataaattcaactttaatgcaggcgacgactatttttatccgcccactcaagcgcataccgttgtttttaacgacaactacgatgcctttcctgagtttttgcaagaatacatcactcaacatcacatccaggccgttgtctgctttggcgacacacgcccttatcacgtcattgcaaaacgcattgcaaacgaaaaccaagccagtttctgggcgtttgaagaaggctatttccgcccctactacatcaccttagaaaaagacggcgtcaacgcattttccccgttgccgcgccgtgccgacttttttcttgaacaattccctaagcttgcccagcaagaatataaagcgccaacgccggtacacggcggttttacgcccatggcaaaaaacgctatccgttactatatcgagttgttccgcaatccacgcaaataccccgactacatccaccaccgcgcacccaatgccggccattacctcaaaccgtggtcgctctccatcctcaagcgtttgaactactatattgaagacatccaaatcgccaaacgtgtggaagcaggcaaatacggcaagttttttattgttcccttacaggtattcaacgacagccaagtccgtatccattgcgactttcccagcgtccgcagcttcctgctccatgttttgagttcatttgccgagcacgcgcctgccgataccaacatcatcatcaagcatcatccgatggaccgcggttttatcgactactggcgcgacattaaacgctttatcaaagaacaccccgaactcaaaggccgtgtgatttatgtccatgatgtccccctgcccgttttcctgcgccacggtctcggcatggtcaccatcaacagcaccagcggcctgtccggactgattcacaatatgccagttaaggttctcggccgtgcctattatgatattcccggcattactgaccaaaataccttggcagaattttggaatcatccgacaccgcctgacaaagagctgttccatgcctaccgaatgtaccacctcaacgtgacccaaattaacggcaacttctacagtcaggtgtttttccccaacaaaaaaacctccaactcttccacaccagtaatctgacttagcgaaggaagttcaggccgtctgaaaacatttcagacggcctgaaacaatcaataccttagctactgccatgtaaataaaacacaaaaatctgcatttatcattaacaataaattacaaaaacagtataatgaccgagctgccatgagcgcataccgactcaacctgagccctttgtaacacacaaaatatggatatatccctaggcaaaacaatataacaagccaaacatcctaaagataagccggcaaggcaatacactctataaaactatgccgagcaaaatttttacaaagccctcaaccggtatcgccgcccatatgccgcagcatccgtcttccactttatatccgcccgcaaaccatgaccgccgctcctgatatcctctaccggcaagccgccgcccttttggaacaatccaataccgcccaagccctgcccctgttgcaacaggcggcagagcaaggttatgcggaagctgctttcgtattgggcaaccatctgctgcaaaacggccaaccggagcaggcactttcatggttggaagccgccgcggcccaacgccatcccaaagcactcttctccctgctgcaacaacgcgaacacaacggcaccccgaccggacagcttctcaacgactatgcctggctgggtgagcaggggcactcagaagcccaattaatcctcatgcgttaccacgcgcaacgcaacgatccacaatcgctctactgggcggaacttgctgccgcccgatatgccgcacctgcgtattaccatctggcacgccatcatcaacgccaaggcgacgttgaaacagccatcgaacaatacgaaaaagcggcagcactcggcgtaactgccgcctgctggcaacttggtcaaatctacttctacggtacaggtgtcagccccaaccacgcacaagccgaacactatctcgaaccagccgcacaagccggccacatcgccgcacaaacgctgctggctgaccttcttgccgcccaacgcaaacctgaagccttggaatggtatcgtcgtgccgccgataaggaacaagcggaagcacagtctaagctggcccaatacgccctgaccggcgaactttccgaacgcgatccgttccaagcggcacgatatgccaaagccgctgccgagaaaaaccatcctgaagccctgaaaatcatgggcgacctctaccgctacggtctcggtatcaaagccgacaaccatatcgcgcaagattactaccaccgtgccgccgcgctgggttctgccgccgcagcacaaaaactcatcagcgacgccgcgctgtaccatccgcaacaatacgaacaaatcaaaactgccgcctgcaacaacaacaaaccgaaaccatctaccgtttggcggaagcacaagcctgcgccatcggccgtcccgccgactacaatgccgcgcgaaaaaattacatggaagctgccgggttccaccataaaaacgcagcggcagccttaggccgcatctaccattacggcctcggtacggcgcaagatcctcgggcggctgcacactggtacgccattgctgccgaacaaaaccacccttccgcccaataccacctcgcctgtttttactatcacgggcaaggtgtcggctgtcatgttccgaccgcctgctactggctgcaggccgccatcggcaacggccacacttcggccgaatcattaatatccctattagaacaatggcgacgcgaagcacaccatgccatcggacaaaaggccgtctgaaaagatttacactcgcattttttgacaatctttaactattcccctaatatttgccagttatttttcacggacacgccattgttttcatttctttctgaaaacaccttgtccgcgcatcaataccatgacactcggcggataacgccaagcgttgaaacacactacatccggaacaaaaacggatgctcggaaaaatatttctaggaggtgaaacaacatggaatgggaattcaacagttattacacactgattgccgccacgctcgtgttgctggttggtaaatttctggttcaaaaaatcaaattcttacgagacttcaatattcccgagccggtagccggcggtttgattgccgctatcgtcctgttcgccctgcacgaggcgtacggcgtgagcttcaaatttgagaaaccgctgcaaaatgcgtttatgctgatttttttcacgtccatcggcttgagcgcggatttttcccgtttgaaggcgggcggtttgccgctggtggtttttaccgcgattgtgggcggatttatcttggtgcaaaactttgtcggggtcggactggctacggctttgggtttggatccgctcatcggtctgattaccggttcggtgtcgctgacgggcggacacggtacgtcaggtgcgtggggacctaattttgaaacgcaatacggcttggtcggcgcaaccggtttgggtattgcatcggctactttcgggctggtgttcggcggcctgatcggcgggccggttgcgcgccgcctgatcaacaaaatgggccgcaaaccggttgaaaacaaaaaacaggatcaggacgacaacgcggacgacgtgttcgagcaggcaaaacgcacccgcctgattacggcggaatctgccgttgaaacgcttgccatgtttgccgcgtgtttggcgtttgccgagattatggacggcttcgacaaagaatatctgttcgacctgcccaaattcgtgtggtgtctgtttggcggcgtggtcatccgcaacatcctcactgccgcattcaaggtcaatatgttcgaccgcgccatcgatgtgttcggcaatgcttcgctttcgcttttcttggcaatggcgttgctgaatttgaaactgtgggagctgaccggtttggcggggcctgtaaccgtgattcttgccgtacaaaccgtggtgatggttttgtacgcgacttttgttacctatgtctttatggggcgcgactatgatgcggcagtattggctgccggccattgcggtttcggcttgggtgcaacgccgacggcggtggcaaatatgcagtccgtcacgcatactttcggcgcgtcgcataaggcgtttttgattgtgcctatggtcggcgcgttcttcgtcgatttgattaatgccgcgattctcaccggttttgtgaatttctttaaaggctgattttccgcctttccgacaaagcacctgcaaggtttaccgcctgcaggtgcttttgctatgatagccgctatcggtctgcaccgtttggaaggaacatcatgtatcggaaactcattgcgctgccgtttgccctgctgcttgccgcttgcggcagggaagaaccgcccaaggcattggaatgcgccaaccccgccgtgttgcaaggcatacgcggcaatattcaggaaacgctcacgcaggaagcgcgttctttcgcgcgcgaagacggcaggcagtttgtcgatgccgacaaaattatcgccgccgcctacggtttggcgttttctttggaacacgcttcggaaacgcaggaaggcgggcgcacgttctgtatcgccgatttgaacattaccgtgccgtctgaaacgcttgccgatgccaaggcaaacagccccctgttgtacggggaaactgctttgtcggatattgtgcggcagaagacgggcggcaatgtcgagtttaaagacggcgtattgacggcagccgtccgcttcctgcccgtcaaagacggtcagacggcatttgtcgacaacacggtcggtatggcggcgcaaacgctgtctgccgcgctgctgccttacggcgtgaagagcatcgtgatgatagacggcaaggcggtgaaaaaagaagacgcggtcaggattttgagcggaaaagcccgtgaagaagaaccgtccaaacccacgcccgaagacattttggaacacaatgccgccggcggcgatgcgggcgtaccccaagccgcagaaggcgcgcccgaaccggaaatcctgcatcctgacgacggcgagcgtgccgataccgttaccgtatcacggggcgaagtggaagaggcgcgcgtacaaaaccagcgtgcggaatccgaaattaccaaactttggggaggactcgataccgacgtgcaaaaagagttggtcggcgaacaacgcaagtgggcgcaggaaaaaatcagcaactgccgacaagccgccgcgcaggcagaccggcaggaatacgccgaatacctcaagctgcaatgcgacacgcggatgacgcgcgaacggatacagtatcttcgcggctattccatcgattaggggcaaaccgatgaataccgtcccaaaaagcaggattcccgtc

附录Baaaccgctgcccgaaaaaaccacagacgaagccaaagtcgaaaaatggcggcagctcggtgcggaacacggtttgtcgggcgaatgggcagttgccgtcagattgggcgaaaacggttttaccgaagaacagatggaaaatatcgccaacctgttcggcagataaagagaaaattgacggaaatgccgtctgaaaccctgttatcggtttcagacggcattttgaccaatacggtacgcaggcgcaaaacagccggcttttcctgtgttgcctatgctgatgtttcaacacacaggacgatacaaaaaacgtcgccctatgtgccgtcctgattcggaagggttacgctccttccaaatatagtggattaacaaaaaccggtacggcgttgtctcgccttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttccgtactatctgtactgtctgcggcttcgttgccttgtcctgatttttgttaatccactataaatcgagcctaaaacaatgccgtctgaaacggaaatctgtttcagacggcattgttacattcaaacggcgggccgtttatttgaatttgtaggtgtattgcagaccgatgatgtcggcgtggtttttgaaacgtgcggaagacgcgcctttgctgtccacatcgttgccgcttgccttcgccgtgcggtagctggtgtcgttgatgtggatgtgggtgtaggcggcatcgacgacgtggtttttaccgatatggtatttcataccggcggagaaccagatgcggttgccgtcgggtaggctgttcatgcggtagtcggcgttgcggacgggcgatttgtcaaaagcgatgccggcgcgcagttgcagcggttcgctgatttgataagaaccgccgaagccgactttgtaggtgttgcgccagttgggggtgatggtggtgcggtcggatttgcctttgacgacggttttttctttttcaaaaaccagttccgccttatcgaagcggctgtggcgcgtccaagttacgtcgccgaacaggtcggctttatcggacactttgtacataccgtgtacggacaaagactcaggcgtaacgattttaacgcgggctttttcattcgccgtgtagccgtttgctgcaagcatcgtactccacattgctttcgccgccgcgccgtctgccgcccattcggcatcgcctttgagcgtgtgcgagactttggaacggtagttcacgcccacgcgcgcacggtcgttgatgtcccacatccacgccagttggtagccgaagccccaatcgctgcctttgacatcggcgtgtccgtcggcctgaatttttgcagcttcggctacaccgttaggtttgggcggttttgccgtcaatatctctgctttactcttaatcccccagtcggcatatttgcgcagttcggcggaagtatgttgggcgatgatgcctgcgccgaaggaatggcggtcgttgagtttccacgcggcgacaggttcgacggcgatgctggtcagaccgagtttgttgatgttgtggcgcaacacggaatctttttcgtattcggtggcagagccgaaggggacgtacacgcccaagcccacggtcagattgtcgttgactttgtatgcgccgtagatgtggggcgcgaccgtggttttggtgattttgccgcttttcgaaccttggacgggaagcccggtaaagtcggtggcggaatccgcctcataatgaatgctgggcagcacgatgttggcgttgacggaaatctggctgctgtcgagtttggtcaggccggcagggttgtagaagatggtcgatgcgtcggcggcttctgcggcggcggcatttgccgtgctttgcgcgttgaccgactgtgtgccgaagtggtagccggatgcgtggacggatgcggcggcaaaggcagtgccgagcagcaggacggtttttttcagtgcggaaggggtcatttcggtttccgtaaaaaggcggacggtggataaatatagtggattaacaaaaatcaggacaaggcgacgaagccgcagacagtacagatagtacggcaaggcgaggcaacgctgtactggtttaaatttaatccactataaaaaaggcagtcggaaatgccttgtttcgctttagtataggtactcgattttatccgatgttgccggatttgcacaattttttcagagtttgcccgaaccgccgcgccgccgcaaaaaatgccgtctgaagcctcgggcatcggcttcagacggcattttccactcagggcggattatttgacgcgcagcacttccagtgtgttggtcgaaccggattcgcgcatttgcgaaccgctggtaatgatgtattggtcgccggaatgcaggatgttgtgttccaccagcatcgtttcgacttcgtttaacgccgtgtcgtggtcggtactggttgccaaaatcagcgggcgcacgccccggtacatcgccatacggcgttgggcggaaacgctcggggtcagcgcgaaaatcggcagggtgatgttgtggcggctgatttcaaaggcggtcgaaccgctttcggtcagggcgacgatggctttggcgtgaaccgcgcgcgccacgctgaccgcaccgccggcaaccgccaggttggtgctgaccgcttcgggatactcgacctgttcggcaacgccgttgagcgaatcctgctctttttccgcagccgcgcagataatcgccatttggctgacggtttcaaacggatacgcgccgacggcggtttcggcggaacacatcaccgcatcggtaccgtccaataccgcgtttgccacatcgctgacttccgcgcgggtcggtacggggttggtaatcatcgattccatcatttgcgtcgccgtaatgctgaagcggcgcaactcgcgggcgcggcggatcatccgtttttgcagggcggggacggcggcgtgtccgacttcgaccgccaagtcgccgcgcgcaaccataatgccgtcgccggcgaggatgatttcgtccaagttttcaatcgcttccacgcgttcgattttggaaaccaaaccggggcgcacggccgtgctgcccttcatttcttcttcgactttggcgcgcgcgatatgcaaatcttcggcggatttcacaaagctgatggcgaggtagtcgcaaccgatggcaatcgcggttttcaggtcgcggaagtctttttcggtcaacgcgcctgcggacagaccgccaccgcgtttgttgatgcccttgttgcttttcaggacgtggctgttttccacccttgtgataatcctgctgccttcgacggattccacggtcagggtcagcaggccgtcgtccagccacaagacatcgcctgcggcaacgtcgtcgggcaggtcgcggtagtccaaaccgaccgcctcgcgcgtgccttcgccttcgagcgcggcatccagtaccagcgtttcgcctttgttcaattcgatgccgccgccggcgattttgcccacgcggattttcgggccctgcaggtcggcaatgatggcgatttcctgtccggcgcgttttgccgcctcgcgcacgatgagggcgttttcctgatggaattcgggcgtgccgtggctgaagttgaagcggacgacgttcagaccgccgacgcggatcatgtcttccaacagttcgacgttgttgctgcccggcccaagggtggcgacgattttagtgttgtggctgatgcgggtcagatcgcggcttgtctggttcatatgaaagtccttttggtctcaatcgggtgttttgcggtattttgttacaaaattacagaaatttggaaccggtttgatgtccatttgatgaacgcggcggaatattctgtaaaaatatgatttaaattaatagtttgatattttacctgcaaaccgccttttttggcgcaaaattacacggttttatgacttaggctaaatttattttggggctgtcctagataactagggaaattcaaattaagttagaattatccctatgagaaaaagtcgtctaagccggtataaacaaaataaactcattgagctatttgtcgcaggtgtaactgcaagaacagcagcagagttagtaggcgttaataaaaataccgcagcctattattttcatcgtttacgatgacttaatttatcaaaacagcccacatttagaaatgtttgatggcgaagtagaagcagatgaaagttattttggcggacaacgcaaaggcaaacgcggtcgcggtgctgccggtaaagtcgccgtattcggtcttttgaagcgaaatggtaaggtttatacggttacagtaccgaatactcaaaccgctactttatttcctattatccgtgaacaagtgaaacctgacagcattttttatacggattgttatcgtagctatgatgtattagatgtgcgcgaatttagccattttagcttcgctgaaacttcgttttcgtatcaatcacagcacacattttgccgaacgacaaaaccatattaatggaattgagaacttttggaaccaggcaaaacgtcatttacgcaagtctaacggcattcccaaagcgcattttgagctgtatttaaaggagtgcgaacgacgttttaacaacagtgagataaaagttcttgttccattttaaaacaattagtaaaatcgagtttatcttagttatctaggacagccccgtttgtgtactgaaatgcttcaaaacaccaaaccaagtttcgttttctaaaatacgaaaccattactgctgcctaaatttttttggattgctaaattatggcagtatgattttggattttaaattgaaaggcaagaaaaatgtcaaaaaatgatgtagttaaagtaattggtatattccccctattgtccgaacaatagagcagacttcccggcaggctgcccacatcagaacgcccgttcgctggtttgtacgtcctgaaaaagctcttgcattaagttaatcataatgggaaatttaaatttttttaatgcttacttaaacaaaagccccactccaccattaggagtttctttttcagtatacaagtaaatatttttaaaatattgatttaatttaaaataaaatacttgcaaaaaaagtattaaattaaacttaagaaaggttaattctgatttacatttccaaccatacttctttacaggagaaaatcatgaaagagttacacacctctgaattagttgaagtgtcaggtggcaaattccatatctttgcacagggtggcggcaacctaggtaaaaaagatatggttgctgttggtaaaattggtgcttcctattcccctaacaatagtggagtagagttttctgttagcaagcaatttggatatgtacaaggtcttggtgtacagttttcgaaacctacttttggtattagtaaaaaatggtaagattttttgttttatcctttctgacattaataaatctatgctcattaagcgcatgcaatagccactttacaggaaatatcaatccattaggtactcacaataaagttgctaatcccaattgtgccaatagtgccaatagtcatatcagacaacccagtaggaaaaactatgatccaactgaatatagtgcttggttacagtatatgcatgattgcaaataatgagtaacgatgaaaatttacttttttctcaaccacacttaacaaaggtgaatattatgcaagttttgactttgaatgaaatttaacaagtttctggtgctgcttgtaactggcgtgatttctcaaaaaataccattggtagtgcattaggtggagcagctggtggggcaattgttggttcatttgcaggtggtattggtgctattccaggtgcgaaattcggagctattggtggtgcaatcactggtgctgtacaatatggaagcacttgttggtggtaatattccttaataaaactagggtattttgatattttctattcaaaataccctagtttttcataagaacttaaatacaaaaaggaacaaataatgaaaaaatatagtgattattttaaatatttaatcttttttttgattttactcccaacaaattatctcgtatctcattatgtggtacaaacctcaatgagtatgttaagcattttaagttcttctataataacaacttgccttttttttgtttttacaaatttatcccaatcaaagaaacataaataagtacacatgtctaacaatcactcatttttcagaccagaagtctttgtagctcaacggaacaagtggacaggaccagtaggctgggttgacgcaatgggagctggtattttctctgttgctggcggatacaatatcggtcgtggcatgatgaagccataagataattacatcattaaggaaaaggtaatttcagttacagcaatatgtattgaagttacctttttctatttagattgaacaattttgaaagagaaaaattatgaatactgaaaccatttacgccactgtcttttgcattttagctgcaaccatttctggattattggttaaatttaatgtaattaaaatagaaacatcaatcaatagcaaatttatgttattaggcataagtattttaattattggtatttttctatccattttt

附录Bttttaagaaataataataaatgtcccacttattccgaaaagaagtctttgtagcccaacaaaataagtggacaggtcaggttatcttgacccgtccattctcttttttatttctgactttttgcgcttttctcattgctctgtgtatcattatctttttgatttttggtagctataccaataaaacaaccgttgaaggtcaattacttccaactatgggggtggttcgtgtttactcttccgatatcggcacgattacgcataaatttgttgaagatggtaactttgtcaaagctggcgaaccattgttcaaactttccacatcgcgttttggcgaaaaaggaaacgtacaagccaaattggcagcagaagccaaccttaaaaaaactttggcattacaagaattggaacgtttaaagcgcattcatcaaaatgagcaaaaaaatgttcataacaacattcatcgtttaaacaatcaattagagaatattaaacagcaaattacagggcaaaatcgtcaaattcgtttagcggaaaaaacccttaacaagaacaagtttttagccagtcaaggcgcagtatcccaacaagataagatgaccgccgaaagccatttattggaacaacgctcacgtttggagagcctaaaacgtgaacaaaataatgcaatcagggaacttgatgaacagaaaatcacattaagcagcctgcctgaacgccataaaaccgaattgagccaactcaaccgtgcgattacggaaatgaaccaagaaattttggattttgatttgaaatccgaacaaaccatacgagctagtaaatcaggttgagacctttgcaaaaataatctgttaacgaaatttgacgcataaaaatgcgccaaaaaattttcaattgcctaaaaccttcctaatattgagcaaaaagtaggaaaaatcagaaaagttttgcattttgaaaatgagattgagcataaaattttagtaacctatgttattgcaaaggtctcaggttatatatcaacaattaatgttgatatagggcaacaagttgaaccgtctaaattgctgttaagcattgtccctgaacaaactgaattggtcgccaatctttacatacccagtaaagctgttggttttattaaaccgaaagataaagttgttttacgttaccaagcgtacccttaccaaaaatttggacatgccacaggagaaattatttcagttgccagaactgctctcggtaaacaaaagctatcaggtttaggtatcattttcactaacccaaccttattaaatgaacctgcctatcttgtgaaagttaaattggaaaaacaaacgattaaagcatacggagaaaacaagccgcttcaaattggcatgattttagaagcagatattctccatgaacgaaaaaattgtacgaatgggtacttgacccactttacagcatttcaggaaaaatcaattaaaaatggattatttatcaagactgtcctttggatttaacaaaaagctacctgtcattctgcaaacagaagttgctgaatgtggtttagcatgcctgacatccatcttgtcctattatggctttcacactgatttaagaacgttacgccaaaaatacaccctgtcattaaagggcgcaaatcttgcagacatcatgagatttggcaatgaaatgaatttaacgccacgagctttgcgtttagagttagatgagctgtcaaatttacaactaccctgcattctccattggaacttaaaccattttgttgtactttgttccatttccaaagacagtatcgtcattatggaccctgctgtcggtatgcgaaaaatcaaaatggacgaagtttcacaaaaattcacagggattgccctagaattattccccaatacccattttgaagagaaaaaagaaacaaagaaaatcaaaatattatctctattaagggggggtcaggcttaaaacgctctttaattcaaatgcttatattagctatttctttggaagtctttgcattggttagtccattctttatgcaatgggtaatagaccatgtcattgtaactgctgataaaaatttattattgacccttactttgggatttggtttactgactatcctgcaacagttaattagcctgttacaagcatgggtaggtatgcacctatctacaactcttaatttacaatggaaagccaatatatttaaaaggttacttgacttacctaatgactatttcagtaaacgacatttaggagatgtgatttcaagatttggttcaatagatcatatccaagaaacactaacttctactttttttgttttagttttaaatagcttaatggctgtttttactttcgtgttaatgacaatttacagcactcaattatcgctgattgttcttttaacacttgttttgtacatactaattcgttggcttgcatattacccattaagaaatgcaacagaagaaaatattgttcatgaagccaaacaaaactcatatttcatggaaaccattcgtggtatccaatcagttaaattatttgataaacattatcaaagacatggcacttggatgagcctatttgtgaatacagtcaataccaagctgacaacagataaactctctgctttatttgaattttcaaataaactgttgtttagcatggaaaatgttatcataatttatcttggtgcaagcgcaattttagatggttcatttacagtcggtgttctgatggcttttttggcttataaagggcaatttgaaagcagaacagcttctctcgttgaccaatacatccaaatcaaaatgttagggcttcatgctgaacgtttggctgacattactttaaatgaaacagaaactgaaattattaagtataatcatatacctaaattagataatgaacaactggttcttaaagttgaaaacgtctcattcagatatgctgataatgagccatatctttttgaaaacattaatttggaatttaaagataatgaagcagttgttttaacaggacaatctggtcgggggaagtccactttgttaaacattttaacaggtagcctaaaacctgaaactggtacagttagtattaatgggcatgatatatatcaagtttctccatcctttattaggggattgagcgggattgttcgccaagatgatgtcctttttgcaggttctattggggaaaatatttcattttttgatgaaagcccaaatatggagctcattgaacaatgtgcaaaaatggcacaaatacatgacgatatacttaaaatgccaatgggctatgagaccttgattggcgatatgggaaatatcttatcaggtggacaaaagcagagagttatcttggctcgtgcattgtataaacgacccaaaattctatttttagacgaagcaagtagccatttagatgtagaaaatgaacaaaaaattaaccataacctaaaaagtcttggtattatgaaaataatggttgcacaccgccaagaaacaattcaatcggcagataaaattctgaatttaggttgaacagaacaagacttcatttttctttaacaaaaagtgaagtcttttttcaaataatttaatagaatacatgaaaatagcggtttaacgttccatttcccaatcatcacgactggctttgtgttttggcgatttttcagtttcctttttctgttgaatttgttgttttttctgctcttgttcccatttttgggctaatttcacggtctcattttcagcccattccatcacggcacaacgatgtagcttttctccgatatcgccattaaagccagctccacgaacttcaccataaattcttgaatatttttgattatattcaatttcttttccattttctttaaaggatttctcccacttttcacaaacttcatcaaaatctttcaaagggatattttttaaggggctgtcctagataactagggaaattcaaattaagttagaattatccctatgagaaaaagtcgtctaagccagtataaacaaaataaactcattgaactgtttgtcacaggtgtaactgcaagaacggcagcagagttagtaggcgttaataaaaataccgcagcctattattttcatcgtttacgattacttatttatcaaaacagtccgcatttggaaatgtttgatggcgaagtagaagcagatgaaagttattttggcggacaacgcaaaggcaaacgcggtcgcggtgctgccggtaaagtcgccgtattcggtcttttgaagcgaaatggtaaggtttatacggttacagtaccgaatactcaaaccgctactttatttcctattatccgtgaacaagtgaaacctgacagcattttttatacggattgttatcgtagctatgatgtattagatgtgcgcgaatttagccattttagcttcgctgaaacttcgttttcgtatcaatcacagcacacattttgccgaacgacaaaaccatattaatggaattgagaacttttggaatcaggcaaaacgtcatttacgcaagtttaacggcattcccaaagcgcattttgagctgtatttaaaggagtgcgaatggcgttttaacaacagtgagataaaagttcttgttccattttaaaacaattagtaaaatcaagtttgtcctagttatctaggacagccccttgttttttgttcggcggcttgcgtggtcgggtaaaatgaaagttttgaacggttggtcggacaggaagatgtggcgggttttgagtgctttgccgataggcgtggtgttttttgatttgatctacggttttgtgttgaatgtgttgcagggtttggatttgcagcgtgccgtgccggattcggaaggcgtgttggcggttacgcccgatattgcattcaacagtttgcagattgtcgccaacggcggtatggcggcggtggtctgtttcgggttggcggttgtgtttttgctcaaccgttcggtgcggcggcggcaggtgttggaaatcggggtgttccggatgttggggctggtggcggtattggcgttcagcgcgccgtcggtgtgggagtgggcgaacgcgctgccgctgctgctgaagggcgcggacgtggtcaatacggggaatgcgcgttatgtgctgacggctttgtgtatgccctttccggcggtgtcgtgcgtcatcgggctggtggggcggttcaggcttcagacggcatcgggcagggcggcaaagtcagggggtgcgggcaaggcggacggataggacgcatttttcagcgggtgcgtcgagaagcagccgatgtgtttggcagccgcagcttggggggtgtagtgctaatggcggtttctttgcttttatagtggattaacaaaaaccagtacggcgttgcctcgccttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttccgtactatttgtactgtctgcggcttcgtcgccttgtcctgatttttgttaatccactatataaaataaatgggcaaaaatcggtttattatcgtttttgccgcatttggatttgttctaccgtaaaacgtgtttgacgaacgggattcttattaaaaaacatctgatttctaacaaaatcagtattttttggcacgatggctaaaatttttccttccatttcgccatcacgtgttttccatgcgctcaagaattgtgatttgctcattgagacgtgccccagcgatggatcagccagcaaaacagtttctccgttaataccgttcaataccgaaaaatggttgtttttacggtattttaaatacacaattacaggaatttttagttgtaccaactgttcaaatggcaaagcataaccttgtgcttcaaaacccagttcgggcattatgcgttgcatatcgtcaaaagaagcacgcatttgggttttatccattttgtctaagatttccgcttcagaataatgtctgccataaaaattattcagtaacgtggcaatcgaagccgcgccgcaagaaaaatccaaatcttgttttactatgccggaatctcgccgtgctttccaactccgtacatggatgttttggtaagaagcggggggtcaacaaacataggccaagcaaaaactatatttggggcgaaaccaatcaaagccgcataatttatcaatttataaagattttttatcataatatgtatacgcggaataaacgcatgaaacataaaaaaataaaatcatatgcaattttattgcatgaataaatatgaataaaatagataatgatgggagtaaatacgccatgtattttggaagtttaaatttattaataataaaataatttatcgtagcgcaaataaatcccaaaattggaacgattagaaaaaaaattaatacacccatcatcatgcaaactctatattaataaatagctaactaattctattgtggaaattagttagctaactaaaagttattaatgattattttcgagaattgactgcattgttggcagcattggcaccaaaacctagtgcatgaatacccggtctccatgccaaattcccagccaatccgcctccggcagcagcagccagccctgttttgccgctactcctgttgccgcaccgat

附录Btcctgtcgcagtagccgcgccttgcgcagttcctaatttaccatgattatacaaattagcaccatgatacccccatgcacctaatgcaccgccaaaagcagcggctgcaataatgggaacaaattcaccttgtgtttctttcatttcagcctgtgataattgaattgctttcacattttggctgtcaaaaacttggctgtctaaattttgcgccattacaggtgtaatcatcatagccattacagttgcaattttcgttgcgctggtttgcacataaataggattagcaaattcgctttgattgcgttcagtgttgatgtagctaatactgctttctagtttgaatttacccttgtcagtcaataattcttccaaacttaaaggtaagtcagcataagcaatttggctggcaacagtcaaaataaaatctattagacatttgtgtttttgcatcatttcgtttgattttctaggttttgagaatgatacaaagttttttacaaagtaaagagtcactctgaaaaaacttttttcattataaatcaaaatattgatagaataaatagcgagcatcgattcacggtgcgctttagtgcaaagcgtacaacgcgagcctgaaccaccagccgcaacaggaaaagaaagccgatagagtgcaatgcttgccaacgtgcaagcgagcttgcaagaacgcttggctcaacgagagcaggcaagacagaaagcagaaagcaggataggagcggtaacgcaaaggtctcgggctttgatttcgccgtaaaccctgctgccgccttgtccggaaagggtgcaggcggcgagtgccgacagggtgcagatggggaggggggttttcatttggggtcgcaacggaagtggtatgcgcagatttcaaaaccgtttttgaaatacaggcggtgcgcgtcggcacggtcgtggttgacgtggacgttgaggtggattttggttacccctgtttccgcgccgattttgcggacttcttccaaaaggcgcgaggcgtagcctttgcggcggctttgcggcagggtaacgatgtcatcgatgtggatgtggcggccgctggcgagggtgcaggcttcgcggaagccgcagacggcgacggcattgtgtttgccttcttcaaaaatacccagcaggcggtagccttgggggcgttggactttgttgatctgttcggtaaagcggttgatgtcggtcagggcggaacgcaaaacgctcaaggctgcaaaggcggtggcggtgtcgtccgcgccgatttcgcgcaaaacgtaggatgcgcccgaggcggtctgttcctgtgctttctcggcggcgtgtttttcttcgattgcctgtgccagcatgacgtgttcgtcggcagggttgttttgtccgccctgttcgcgttcttcgagcagggctttgcagtcgatgacgcgcaggtcgttgtcggcggcaaagtccatcaggaagcggaacatttggggattgtcggtttccagttttttatcgacggcgacgcagcggatgttgtccaccagtatggggcgcacccatttcgaataggaaagcctgtggtctttggtgaacgaggacagaatgccgcacaggcgttccgcccagtcgctgggacggaaaatcttgccggaactcgttgtgccgtggatgacgacttcgtaggggttgcagactaacatggcggcttcctgaaaagaaatgtctagcgcgattataccttatgcttatgcgggcgtgtttggatatgccgtctgaaaagtacgggattcgtgcggtaaaactttgcggcggcaaatgtgcgataatacgcgccgtattgccgcttttgcgaagctgttccgcaaacatacgggcggcgtggacgacgtataaccggatacccgcctgacgcgggttttttacggaaggggggcaaaaatgcctaatccgctttacagacagcatatcatctccatttcggatttgtcgcgcgaacagttggaatgcctgcttcagacggcattgaagctgaaggcgcatccgcgcggcgacctgttggaaggcaaacttatcggttcgtgctttttcgagccgtccacgcgcacgaggctgtcgtttgaaacggcggtgcagcgtttgggcggcaaggtcatcggtttctcggacggcgcgaataccagtgccaaaaaaggcgagacgcttgccgataccgcccgcatcatttccggatatactgatgctatcatccaacgccaccccaaagacggcgcggcgcgcgtggcagcggagttttcgcgcgtccccgttatcaacgccggcgacggcacgaaccagcaccccagtcagacgctgctcgacctggttaccatttatgaaacacagggacgtttggacaagctcaaaatcgccatggcgggcgacttgaaatacggacgtaccgtgcattcgctttgtcaggcgttgaaacgctggaattgtgaatttgcctttgtttcgccgcccagcctagccatgcccgactatattaccgaagagttggacgaagccggctgccgataccgtatcctcggtagtttggaagaagcggcggaatgggcggatatcctgtatatgacccgcgtccagcgcgaacgtttcgacgaacaggaatttgccaaaatccaaggcaaattcaacctcgaagcgtctatgctcgcccgcgccaaaccgaacctgcgcgtgctgcaccccctgccgcgcgtggacgaaatccatcccgatgtcgatgccacgccgcacgcctattatttcgagcaggcgaccaacggcgtttatgcgcgtatggcgatattgtcgctggtgttgaacgaagaagtgtgaggaaccgatatggaaaccccgaaactcagtgtcgaagccattgaaaaaggtacggttatcgaccatattcccgccggcagggggctgaccatcctgcgccagttcaaacttttgcactacggcaacgcggtaaccgtgggcttcaacctgcccagcaaaacccaaggcagcaaagacatcatcaaaatcaaaggcgtgtgcttggacgacaaagccgccgaccgcctcgccctgttcgcccccgaagcggtggtcaacaccatcgacaatttcaaggtcgtgcagaagcggcatttgaacctgcccgacgaaatcgccgaagtgttccgctgtccgaacacgaattgcgccggccacggcgagccggtcaaaagccggttttatgttaaaaagcacaacgggcagacgcggctgaaatgccactactgcgaaaaaacctacagccgggattcggtggcggaagcctgacggattcccttaaaccgagtgggcggcatttcgtctgccgcctgttttgccaatctgaaatggaatgatgatgcacgcttctgtccaaagccgtttcgcaccgatactttatgttttgattttctttgccggttttttgaccgcgcaaatctggttcaatcagaaagcctatactgaagagctgcctccgcttctgtccgcattgtccgccgtcgcgctggtgtggctggcgtgggcgttcgtgtcggcgcgttcaaaggccaaggcggaaaagttctaccgcgaaaaaatgatacagaacgaaagcatacaccccgtcctgcacgcctctttgcaacacttggaacacaagccgcaaatactcgccctgctggtcaaaaaccacggcaaagggatggcggaacaggtcaggttcaaggcggaagtgctgcccgacgacgaagacgcgcgcacgattgccgccgagttggcaaaaatggatatgttcgcattggggacggacgcggtcgcctcgggcgaaacctatggacgcgtgttcgccgatattttcgagttgtcggcggctttggaagggcgcgcgttcaaaggaatgttgaaactgacggcggaatataaaaacatcttcggcgatgcctgccgttcggaaacggcgttggagttgggcgcactcaatcaggcgttgcaggagatttcaaaaacatcggaaaagtccaaacggatattttattgaagatggaaaaatgccgtctgaaacggaaggtgtttcagacggcatttttgtcggatgattaattattcggagcggttgaagccaaacttcacgcggctgcggccctgatccggtatattgtccaaatcgcgtcccggattggcggcggtgtcgcctacggaaatatcggagatgttttccaaaatgatggcggacgacaggtgttcggaggtgcggtaaaccattgccaagcccacttcttcggcaggagtggaaatcagctcgacggtatccctgcttttgaaattgttggagaggtcgacctgcatcgttttcttgcgtttgtagaggctcaaaaccgtgcctttgtccaaaccgtccgcctcgcctttgtcgatggtgatggtttgaaactggccggcaatccttgtgccttcaaacacggaaacgattttagcctgaaccgggcgggacggttcgtgcggcatcatgttgaagcggtcggtgtcttccggcattttcatcaggtagtcgccctgctgtatttcggaaatggcggtttcgaccaccagcggctgtatcgaaggggtgcgcagcggggtaatcaaaggatgggtgcgggtatggtattcgttgtctttcggccgttctccagcctgtttcgagcgttgttcgaggacagagtcggtatagtcgagggagcgcacgatgccgctgaatgcgacttcctgcccgaggaatttacccgtatccggatcggtgatgtttttattgattcggtaggtcaggtagcggcccggctctttcaggcctttggtgtaaaccctggtgcctttggtgtacagcagcctgccttccgggcccgagagcaggcgcggcgcggcagcggtttctttgcgggaaacgatttgcggatgccgcataaagatgcggtagaagttgacatcgatggcgggaataccgtatccggacacttccttatccggactcattttgacgacggggatgccgtctgtctgttccaagccgaggcgcggttcgccgtcaacgtggcgcaacaccaatacctggtccggataaatcaggtcgggattgtggatttgatcccggttcgcgtcccacaggcggccccattgccacgggctgtacaggtatttgcccgaaatgccccacagggtgtcgccctgtttgaccgtgtagcgttccggcgcgttcgggcgcacctccaaatttgccgccaaagtttgtgttgagaatgccatacctgccgcgcagagcagggttataatacgacgttgcataaccgttccccttatctgataaatttcggtttgtcttgcttgattgggttggaaaaagcggcggcagcccctcgggatgtgccgcgtgataaaaaatgttccgcattttaacatcgaattatccgcaccatcacggtaattatgaaaaacaggcggcgtatccgccgaaggaaagagaaaattatggctttattgaatatcttgcaatatcccgacgagcgtctgcacacggtggcaaagcctgtcgaacaagtcgacgagcgcatccggaagctgattgccgatatgtttgaaacgatgtacgaatcgcgcggcatcgggctggcggcgacgcaggtcgatgtgcacgagcgcgtggtcgtgatggatttgaccgaagaccgcagcgaaccgcgcgtgttcatcaaccccgtcatcgttgaaaaagacggcgaaaccacttacgaagagggctgcctgtccgtgccgggcatttacgacaccgtaacccgcgccgaacgcgtcaaggtcgaggctttgaacgaaaaaggcgaaaagttcacgctggaggcggacggcttgttggcgatttgcgtgcagcacgagttggaccacctgatgggcatcgtgtttgtcgaacgcctttcccaactcaagcaggggcggattaagaccaagctgaaaaaacgtcagaaacatacgatttgacccttttgccgtgccgtctgaacgctgcaaagttttcagacggcacggtcttgtccgacaattttacgcacgcgcaggaacacgctatgaaagtcatcttcgccggcacgcccgattttgccgccgccgccttaagagccgttgccgccgccggttttgaaattccgctggtgctgacccagcccgaccgtccgaaagggcgcggtatgcaactgactgccccgcccgtcaaacaagccgcgctggaactcggtttgcgcgtcgaacagcccgaaaagctgcgcaacaacgccgaagccctgcaaatgctcaaagaggtcgaggcagacgtaatggtggttgccgcctacggtttgattctgccgcaggaagtgttggatacgccgaaacacggctgcctcaacatccacgcttcgctgttaccccgttggcgtggcgcggcgccgattcaacgcgcgattgaagccggcgatgccgagacaggcgtgtgtattatgcagatggacatcggtttggacaccggcgatgtggtcagcgaacaccgctacgccatccaaccgaccgataccgccaacgaagtccacgacgcgctgatggaaatcggtgcggcggcggttgttgccgatttgcaacagcttc

附录Baaagcaaaggccgtctgaacgcggtcaaacagcccgaagaaggtgttacttacgcgcaaaaattgagcaaagaagaggcgcgtatcgattggagcaaaagcgcggcggttatcgaacgcaaaatccgcgccttcaaccccgtgcctgccgcgtgggttgagtatcagggcaagccgatgaaaatccggcgggcggaagtggtggcgcaacaaggcgcggcaggcgaagtgttgtcctgttcggcggacggtttggtcgttgcctgcggcgaaaacgcgctgaagattaccgaattgcagcctgccggcggcaggcggatgaatatcgcggcgtttgcagcaggacggcatatcgaagcaggggcgaagctgtaaatcccttcagacggcattccgatccgcaaacgggaatgccgtctgaaaccatcagtcgaagaaagcgaatcacataatatgagtatggcacttgcccaaaaacttgccgccgacagcattgcggcggttgccgaaggacgtaaccttcaggacgtgttggcgcaaatccgcaccgcgcatcccgaccttatggcgcaggaaaacggcgcgttgcaggacatcgcctacggctgccagcgttatttgggcagtttgaaacatatgctcgcgcagatgctgaaaaagccgattggcaatccgcagctcgaaagcctgcttttggcggcgttgtaccagctgcattacacgcgcaacgcgccccacgccgtggtcaatgaggcggtggaaagcatcgcgaaaatcggacgcgggcagtaccgttcgtttgccaacgcggttttgcgccgctttttgcgcgaacgcgacaagcttgtggcttcctgtaaaaaagacgatgtagcgaaacacaacctgccgctgtggtgggtggcttacttgaaaaaccattatccgaaacactggcacaacatcgccgccgcgctgcaatcccatccgccgatgactttgcgcgtcaaccgccgacacggcaatgccgaaagctatttggaaaaactggtggcggaaggtatcgcggctaaggcgttggacgaatatgcggttacgttggaagaagccgtgccggtgaaccgcctgcctggtttttcagacggcattgtttcggtacaggacttcggcgcgcagcaggcggcgtatttgttaaacccgaaagacggcgaacggattttggacgcgtgcgccgcgccgggcggcaagacggggcatatcttggaactggcggattgccgtgttaccgccttggacattgatgcaggccgtctgaaacgggtggaagacaatatcgcgcgtctgggctttcagacggcatcgacggcgtgtgccgatgcacaggacctgtcggcatggtatgatgggaaaccgtttgatgccgtccttgccgacgtgccgtgtaccgcctcgggcgtggcgcggcgcaatcccgacgtgaaatggctacgccgtccgaccgacgcgctcaaaaccgcccgccagcaggaagccctgctagatgcattgtggcaggtgctgaaaagcgggggaaggatgttgatcgctacctgttccgtgttcgtcgaggaaaacgacggacaattgcaaaaattcctcaaccgccatgccgatgcagaactgatcgaatcgcgggtactcttaccgaacaaacaccaagatggcttttattacgcgcttattcaaaagcagtaaatggctgattgtgccgctgatgctccccgcctttcagaatgtggcggcggaggggatagatgtgagccgtgccgaagcgaggataaccgacggcgggcagctttccatcagcagccgcttccaaaccgagctgcccgaccagctccaacaggcgttgcgccggggcgtgccgctcaactttaccttaagctggcagctttccgccccgataatcgcttcttatcggtttaaattggggcaactgattggcgatgacgacaatattgactacaaactgagtttccatccgctgaccaaccgctaccgcgttaccgtcggcgcgttttcgacagactacgacaccttggatgcggcattgcgcgcgaccggcgcggttgccaactggaaagtcctgaacaaaggcgcgctgtccggtgcggaagcaggggaaaccaaggcggaaatccgcctgacgctgtccacttcaaaactgcccaagccttttcaaatcaatgcattgacttctcaaaactggcatttggattcgggttggaaacctctaaacatcatcgggaacaaataatgcgccgttttctaccgatcgcagccatatgcgccgtcgtcctgttgtacggactgacggcggcaaccggcagcaccagttcgctggcggattatttctggtggattgttgcgttcagcgcaatgctgctgctggtgttgtccgccgttttggcacgttatgtcatattgctgttgaaagacaggcgcgacggcgtattcggttcgcagattgccaaacgcctttctgggatgtttacgctggttgccgtactgcccggcgtgtttctgttcggcgtttccgcacagttcatcaacggcacgattaattcgtggttcggcaacgatacccacgaggcgcttgaacgcagcctcaatttgagcaagtccgcattgaatttggcggcagacaacgccctcggcaacgccgtccccgtgcagatagacctcatcggcgcggcttccctgcccggggatatgggcagggtgctggaacattacgccggcagcggttttgcccagcttgccctgtacaatgccgcaagcggcaaaatcgaaaaaagcatcaacccgcacaagctcgatcagccgtttccaggtaaggcgcgttgggaaaaaatccaacgggcgggttcggtcagggatttggaaagcataggcggcgtattgtacgcgcagggctggctgtcggcgggtacgcacaacgggcgcgattacgccttgtttttccgtcagccggttcccaaaggcgtggcagaggatgccgtcttaatcgaaaaggcaagggcgaaatatgctgagttgagttacagcaaaaaaggtttgcagacctttttcctggcaaccctgctgattgcctcgctgctgtcgatttttcttgcactggtcatggcactgtatttcgcccgccgtttcgtcgaacccgtcctatcgcttgccgagggggcgaaggcggtggcgcaaggcgatttcagccagacgcgccccgtgttgcgcaacgacgagttcggacgcttgaccaagttgttcaaccacatgaccgagcagctttccatcgccaaagaagcagacgagcgcaaccgccggcgcgaggaagccgccaggcattatcttgaatgcgtgttggaggggctgaccacgggcgtggtggtgtttgacgaacaaggctgtctgaaaaccttcaacaaagcggcggaacagattttggggatgccgcttacccccctgtggggcagcagccggcacggttggcacggcgtttcggcgcagcagtccctgcttgccgaagtgtttgccgccatcggcgcggcggcaggtacggacaaaccggtccatgtgaaatatgccgcgccggacgatgccaaaatcctgctgggcaaggcaaccgtcctgcccgaagacaacggcaacggcgtggtaatggtgattgacgacatcaccgttttgatacacgcgcaaaaagaagccgcgtggggcgaagtggcgaagcggctggcacacgaaatccgcaatccgctcacgcccatccagctttccgccgaacggctggcgtggaaattgggcgggaagctggatgagcaggatgcgcaaatcctgacgcgttcgaccgacaccatcgtcaaacaggtggcggcattgaaggaaatggtcgaagcattccgcaattatgcgcgttccccttcgctcaaattggaaaatcaggatttgaacgccttaatcggcgatgtgttggcattgtatgaagccggtccgtgccggtttgcggcggagcttgccggcgaaccgctgacggtggcggcggatacgaccgccatgcggcaggtgctgcacaatattttcaaaaatgccgccgaagcggcggaagaagccgatgtgcccgaagtcagggtaaaatcggaaacagggcaggacggtcggattgtcctgacggtttgcgacaacggcaaagggttcggcagggaaatgctgcacaacgccttcgagccgtatgtaacggacaaaccggcgggaacgggattgggtctgcctgtggtgaaaaaaatcattgaagaacacggcggccgcatcagcctgagcaatcaggatgcgggtggcgcgtgtgtcagaatcatcttgccaaaaacggtaaaaacttatgcgtagcagcgatattttaattgtagacgacgaaatcggcatccgcgacctgctgtcggaaatcctgcaggacgaaggttattcggtcgcattggcggaaaacgccgaagaggcgcgcaagctgcgccatcaggcgcgccccgcgatggtgctgctggatatttggatgcctgattgcgacggcatcacccttttgaaggagtgggcgaaaaacgggcagctcaatatgccggtggtgatgatgagcgggcatgccagcatcgataccgccgtggaagccaccaaaatcggcgcgatcgattttttggaaaaaccgatttccctgcaaaagctgctgtctgccgtcgaaaacgcgttgaagtacggtgcggcgcaaaccgaaacggggcctgtattcgacaagctgggcaacagtgcggcgattcaggaaatgaaccgtgaggtaggggctgcggtgaaatgtgcctctcccgtacttttgacgggcgaggcgggttcgccgtttgaaacggtggcacgctatttccataaaaacggtacgccgtgggtcagcccggcaagggtcgaatatctgatcgatatgccgatggaactgttgcagaaggcggagggcggcgttttgtatgtcggcgacatcgcccagtacagccgcaacatccaagccggtattgcctttattgtcggaaaggcggaacaccgccgcgtcagggtggtcgcatcgggcagcagggcggcaggttcagacggcattgcctgcgaggaaaagctggcggaactgctgtcggaatcggtcgtccgtattccgccgctgcgtatgcagcatgaagacattcccttcctgatacaggggattgcctgcaatgtggcggaaagccaaaagattgcgcctgcctcattcagtgaagaggcacttgccgcattgacccgttacgactggccgggaaatttcgaccaactgcaaagcgtcgttgcaacgctgttgttggaggcggacggacaggaaatcggcgcaggggcggtttcttcccttttggggcagaatgtgcctgccgagggggcggaagatatggtgggcgggtttaatttcaacctgcccctgcgcgaattgagggaggaggtggagcggcgttatttcgagtaccacatcgcccaagaaggtcagaatatgagccaagtggcgcagaaagttggtttggaacgcacgcacctttaccgcaaactcaaacagctcggcatcggcgtttcgcgccgggcgggggaaaaaaccgaagaataggcccggacggccggtttaccggctgcgggcttttgttttcagacggcatttggtgcaaatgccgtctgaaatcgtaaggggacggattttatgacagaggacgaacgtttcgcgtggctgcaattggcgtttacgccctatatcggcgcggaaagtttcctgctgctgatgcgccgtttcggcagcgcgcaaaatgccctgtccgcaccggcggaacaggtggcggcactgatacggcacaaacaggcgcttgaggcttggcgcaatgcggaaaaacgcgctctggcgcggcaggcggcagaagcggcattggaatgggaaatgcgggacggatgccgcctgatgctgcttcaggatgaagattttcccgaaatgctgacgcaggggctgaccgcgccaccggttttgtttttgcgcggcaacgtgcaactgctgcacaaaccttccgccgccatcgtcggcagccgtcatgccacgccgcaggcgatgcggattgccaaagatttcggcaagtcgttgggtgggaaaggcattcccgttgtgtcgggtatggcttcgggcatcgataccgccgcccatcagggtgcgttgcaggcagaaggcggcaccatcgccgtgtgggggacgggcatagaccgcatttatccgccggtcaacaaaaaccttgcctatgaaatcgccgaaaaaggattgattgtcagcgagttccccatcggcacgcggccgtatgccggcaattttccgcgccgcaaccgcctgattgccgccctgtcgcaagtaacgctggtggttgaagccgcgttggaatccggttcgctgattactgccagattggcggcggagatggggcgcgaagtgatggcggtacccggctcgatagacaatccacacagtaaaggctgccacaaactgattaaagacggcgcaaaattggtggaatgcctggacgacatcctgaacgaatgcccggggctattgcaaaatacgggtgcttca

附录Btcatattctataaataagggaatacctgaaaagcgcatcactgccgttcagacggcatccgaccagctgtctctgcctgaaggcaaaatgccgtctgaaaagacggagaaccgacccgtcggcggcagtatcttggacaggatgggtttcgacccagttcatcccgacgtgcttgccggacagttggctatgcctgccgcagatttgtatgccgcactgttggaattggaattggacggcagcgttgccgcaatgcccggcggcagataccagcgtatccgaacttgaacgcactttatattaaggaacacgaatgaccgaagtcatcgcctacctcatcgaacatttccaagatttcgatacctgcccgccgcccgaagacttgggtatgctgcttgaagaagcgggtttcgatacgatggaaatcggcaacaccctgatgatgatggaagtattgctcaacagctccgaattttccgccgaacccgccgacagcggcgcattgcgcgtgtacagcaaagaagaaaccgacaacctgccgcaggaagtgatggggctgatgcagtatctgattgaagaaaaagccgtcagctgcgaacagcgggaaatcatcatccacgcgctcatgcacattccgggcgacgaaattaccgtagataccgccaaagtgctgaccctgctgcttttatgggcaaacaagagcgagctgcccgtgttggtcggcgacgagctgatgagcgcgcttttactcgacaacaaacccacgatgaactgaagcggcttcagacggcccgcccgagtccgtctgaaacgtcggcatcaaaaccaccatccagagaacgacaaatggcgaaaaacctattaatcgtcgaatccccgtccaaagccaaaaccctgaaaaaatatttgggcggcgattttgaaatccttgcatcctacggacacgtccgcgacctcgtccccaaaagcggcgcggtcgatcccgacaacggctttgcgatgaaataccaactcatcagccgcaacggcaaacacgtcgatgccatcgtcgccggtgccaaagaagctgaaaacatctacctcgccaccgacccggatagggaaggcgaagccatttcctggcatcttttggaaatcctcaaatccaaacgcggcttgaaaaacatcaagccgcagcgtgtcgtgttccacgaaatcaccaaaaacgccgtgctcgatgccgttgcccatccgcgcgaaatcgaaatggacttggtcgatgcgcaacaagcccgtcgcgctttggactatttggtcggtttcaacctttcgccattgttgtggaaaaaaatccgtcgcggtttgagcgcgggccgtgtacaaagccccgcactgcgtttgatttgcgaacgcgaaaacgaaatccgcgcgtttgaagcgcaggaatattggacggtacatctagacagccacaaaggccgcagcaagttcaccgccaaactcgcccaatacaacggcgcgaaactcgaacaattcgacctgccgaacgaagccgctcaagccgatgtgttgaaagaactcgaaggcaaagaggccgtcgttaccgccatcgaaaagaaaaagcgcagccgcaaccccgccgcgccgtttaccacatccaccatgcagcaggatgctgtgcgcaaactcggcttcaccaccgaccgcaccatgcgtaccgcccagcagctttacgaaggtattgacgtagggcagggtgccatcggtctgattacctatatgcgtaccgacagcgtgaacttggcggatgaagccttaaccgaaatccgccattacattgaaaacaaaatcggcaaagaatatctgccgagtgccgccaaacaatacaaaaccaaatccaaaaacgcccaagaagcgcacgaagccatccgcccgacttccgtgtaccgcacgcccgaaagcgtcaaacccttcctgagcgccgaccagttcaaactctatcaaatgatttggcagcgtaccgtcgcctgtcagatgacgcccgccaaattcgaccaaaccaccgtcgatattaccgtcggcaaaggcgtattccgcgtaaccggacaagtgcaaaccttcgcaggcttcctcagcgtttacgaagaaagcagcgacgatgaagaaggcgaagacagcaaaaaactgcccgaaatgagcgaaggcgacaaattgcccgtggacaaactctacggcgaacaacactttaccactccgccgccacgctacaacgaagccacgctggttaaagccctcgaagaatacggcatcggccgcccctcgacctacgccagcatcatctccacgctcaaagaccgcgaatacgttacccttgagcaaaaacgctttatgcccaccgacacaggcgacatcgtcaataaattcctgaccgaacacttcgcccaatacgtcgattaccacttcactgccaaactcgaagaccagcttgacgaaattgccgacggcaaacgccaatggattcccttgatggacaaattctggaaaccgttcatcaaacaagtggaagaaaaagaaggcatcgaacgcgccaaatttaccacgcaggaacttgatgaaacctgcccgaaatgcggcgaacacaaactgcaaatcaaattcggcaaaatgggtcgttttgttgcgtgtgccggttatcccgagtgcagctacacgcgcaatgtcaacgaaaccgccgaagaagctgccgaacgcatcgccaaagccgaagccgaacaggccgaactcgacggacgcgagtgcccgaaatgtggcggtcgcctagtgtacaaatacagccgcaccggcagcaaattcatcggctgcgtcaactatccgaaatgcaaacacgtcgagccgctggaaaaaccgaaagataccggcgtccagtgtccgcaatgcaaaaaaggcaacctcgtcgagcgcaaatcccgctacggcaaactgttttacagttgcagcacctatcccgactgcaactacgccacttggaacccgcccgttgccgaagaatgcctgaactgccattggccggtcttgaccatcaaaaccactaaacgctggggtgtagaaaaagtctgcccacaaaaagaatgcggctggaaagaacagattgaaccgcccgcgccgaaggagtaagattaggttggtttgaaagagaaaaggtcgtctgaaaaattttcagacgacctttgcttttctgtgattggtttatttgaatccgcgtgttgttttaaagtccgataaaatccggttcatttcaggcgcaaacaaggcgatgtaatcgtaagatagaccgcgactggcactgggatggggaaagcagacgacttcgcaatcttcaaacgattggaatttgacattgaaacgtgtaccgtcaaattctttttgcaccgtctccagcggtttggtctgcttaccgaccaactgctcgaagcgtggcagtacattttggttgttcagaaaatccgccaacctgctgcccatgaagaggatgactttcggacgcagtttttcgatgtggtagagaaaattatcgatgtgctcgggttgtgtgaacttgtcgggattgtcgatagtgttgccctgtgtagcagcccagttggtttgaaccagggatttttcaaatgcaccgcccaatccattttcgtctaaggggtgtccccacatttcaaaccaattttttatcgtattgtcgtaacgccacttttttgcctgctctccgaaatagagggatttgtttgcaaatgtatggtcgattttgttttcagggagtttgtattcacctgctacataagcagcctcatcggctttactccaaccccattcatagccacaaatcattaagccatgtttgtcgttgtagcctttgaacaggctgttgctcaaattcaaatccttcatcatgaactcttccttttaaaatttaagagcgattgacttcaatgtttttagatggggtggaaaaatccttgtgtaggcaacataaattcaataaatttcttgataattcgaaacctactaatagcgcacctataaaagctttttcattacgttcagcatgacggtcacgtcgttcatattttttacgcttgctgttccctgttattacagctaagccaagtgatatggcgagaattgcccaaacaatagtacttaataacaattttccccatactatcaataaggaaagaaaaaaccttttagtattagatcgataggttataatccatgcccatgaaaatgcaataagagttatacataagatacaagctgacaggatttctttatttttaattaaataacttagagcgatgaggatgacaggtgtcaagaaaataatagttacatcccgatagctataaaagaaaactgccctattttgaatgtggagatgtgcacagaatccaatatagctaaggataatagttaatataataaaaaaagaccaccaagggtgaagagataggaattccatgttttccctttttttgtaaaaaggaaaaaatcttatctaatagttaattgcctaaccagccagaagaagtttaaaatctatcccaataattcaaccatctatacagaaagttcagcttatggaaacccacgaaaaaatccgcctgatgcgcgaattgaataaatggtcccaggaggatatggcggaaaagctggcgatgtcggcaggcgggtatgccaaaatcgaacggggcgaaacgcagttaaatatcccgcgtttggagcagttggctcagattttcaaaatcgatatgtgggacttgctcaaatcgggcggtggtgggatggtgtttcagattaatgaaggtgatagtggtggcgatattgcgttgtatgcgtcgggtgatgtttcgatgaaaatagaatttttaaaaatggagttgaaacactgcaaagaaatgttggaacaaaaagacaaagaaatcgagctgctccgcaagctgaccgaaaccgtttaaacagatatgccgtctgaaaaaagttttcagacggcatattctttgacaggtcttgtataataccgtttgaacttacaggtttttgattatggcggcaggcaaacataccaaacacagcaaccgggtacgcattatcggcgggcaatgccggggcaggaaattgagtttcacatccgccgacggactgcgtccgacacccgacagcgtgcgtgaaaagctgtttaactggctgggacaggatttgacgggtaaaacggttttggatctcttcggaggcagcggcgcactcggtatagaagccgcttcgcgcaacgccaaacgcgtgctgatttcggataacaaccgccaaaccgtgcagaccttgcagaaaaacagtcgcgaactgggtttggggcaggtgcaaatcgtcttttcagacggcatcgcatatttgaagaccgtatccgaacagtttgatgttgtctttctcgacccgccgtttgcatggcaggactggcaaatcctgttcgatgccttgaagccgtgcctgaacccccgggcattcgtctatctcgaggcgggtacgctgccgaatattcccgattggctgacggaatatagagaagggaaatcggggcagagtacatttgaattaagggttttccaagtggctgaataatatgcgctttgataatcatttccgagttgtaaacattcgtttgcaaccgtccggttcaaaaaaaccttgtgctataatccgcgcccgcccggttttgataatttagtggaaaaggaaaagaaatgtcgctttttattaccgacgagtgcatcaactgcgacgtatgcgaacccgaatgccccaatgatgccatttcccaaggcgaggaaatttacgaaatcaaccccaacctctgcacgcagtgcgtcggacactacgatgagccgcagtgccagcaggtttgcccggtggactgcatcctgattgacgaagaacatcccgaaacccatgacgagttgatggcgaaatacgaaaagattatccagtttaaataaattctttttaaaacatcaaattatgtctgttttgaaataaaatcaaaaaaaaacttgacggaaaagcaagccgctaataaactaacgttctcttttggagggattcccgagcggtcaaagggggcagactgtaaatctgttgcgaaagcttcgaaggttcgaatccttctccctccaccaaaattcttacttggggcagtagcgagtaatgcgggtgtagctcaatggtagagcagaagccttccaagcttacggtgagggttcgattcccttcacccgctccaaacaattaggcccatgtagctcaggggtagagcactcccttggtaagggagaggtcggcagttcaaatctgcccatgggcaccatctctcgattattcatttctttaaggcttagatatataggatattgccatggctaaggaaaaattcgaacgtagcaaaccgcacgtaaacgttggcaccatcggtcacgttgaccatggtaaaaccaccctgactgccgctttgactactattttggctaaaaaattcggcggtgctgcaaaagcttacgaccaaatcgacaacgcacccgaagaaaaagcacgcggtattaccattaacacctcgcacgtggaatacgaaaccgaaacccg

附录Bccactacgcacacgtagactgcccggggcacgccgactacgttaaaaacatgattaccggcgccgcacaaatggacggtgcaatcctggtatgttccgcagccgacggccctatgccgcaaacccgcgaacacatcctgctggcccgccaagtaggcgtaccttacatcatcgtgttcatgaacaaatgcgacatggtcgacgatgccgagctgttggaactggttgaaatggaaatccgcgacctgctgtccagctacgacttccccggcgatgactgcccgattgtacaaggttccgcactgaaagccttggaaggcgatgccgcttacgaagaaaaaatcttcgaactggctgccgcattggacagctacatcccgactcccgagcgagccgtggacaaaccgttcctgctgcctatcgaagacgtgttctccatttccggccgcggtacagtagtaaccggccgtgtagagcgcggtatcatccacgttggtgacgagattgaaatcgtcggtctgaaagaaacccaaaaaaccacttgtaccggtgttgaaatgttccgcaaactgctggacgaaggtcaggcgggcgacaacgtaggcgtattgctgcgcggtaccaaacgtgaagacgtggaacgcggtcaggtattggctaaaccgggtactatcactcctcacaccaaattcaaagcagaagtatacgtactgagcaaagaagagggtggtcgtcacactccgttcttcgccaactaccgtccgcaattctacttccgtaccaccgacgtaaccggcgcggttactttggaagaaggtgtagaaatggtaatgccgggtgaaaacgtaaccatcaccgtagaactgattgcgcctatcgctatggaagaaggcctgcgctttgcgattcgcgaaggcggccgtaccgtgggtgccggcgtggtttcttctgttatcgcttaagtttagaggccaatagctcaattggtagagtatcggtctccaaaaccgagggttgggggttcgagaccctcttggcctgccaaataaaaaattaaccggccttgtgtcggttaatttttttgtatttgttatttagtaaactctcttgccatttacatggattgagaatagacagatgctatgatggataaataatatgacagaacatacgcctgaaaaaaagaacgttaaagtggatcaactggttgttcaagataaagaatctgcatctaattccggtaaggaagggttttttgcatatttctcaaattcttggtccgaattcaaaaaggtggtttggcctaagcgtgaagatgctgtcagaatgactgtatttgttatagtgtttgttgctgtgctttctatatttatctatgcggcagatacagcaatttcgtggttattttttgatgtattgctgagaagggaaggttgagatgtcgaaaaaatggtatgttgtacaggcgtattcggggtttgagaagaatgtccaacgaatattggaagagcgcattgcccgtgaggagatgggagattatttcggacaaattctggtgcctgtagagaaagttgttgatatccgcaatggtcgtaagactattagtgaaagaaagtcatatcctggttatgtgctagttgagatggaaatgacagatgactcttggcatcttgtaaaaagcaccccccgtgtttccggttttattggagggagggctaatagacctacgccgattagtcagagagaggctgaaattattttacagcaggttcagaccggcatagagaagccgaaaccaaaagttgaatttgaggtcggtcaacaggttcgtgtaaatgaagggccgtttgcggattttaacggggtggttgaggaggtcaattatgaacggaataagttacgcgtgtctgttcagatatttggtagagaaacacccgttgagctggagttcagccaggttgaaaagattaactgatttttatacttgaaaaaaaagcaataagaggatagaatcaaaaattaacttggggagcggaaatggttccgcgtcttacccgtttttaggagttcgttaagtggcaaagaaaattatcggctatattaaactgcaaattcctgcaggtaaagccaatccatctcctccggttggtcctgctttgggtcagcgcggtttgaatattatggaattttgtaaggcatttaatgctgcaacccaaggtatggagcctggcttaccgattccggttgtgattactgcatttgcagataaatcattcacatttgtgatgaaaaccccgccagcttctatcttgttgaaaaaggctgccggtttgcaaaaaggtagttctaatcctctgaccaacaaagtgggtaaattgacccgtgcccagttggaagaaattgctaaaactaaagatcctgatttgactgctgctgacttggatgcggctgtccgtactatagcaggttctgctcgctcaatgggcttggatgtggagggtgttgtataatggctaaagtatctaaacgcttgaaagctcttcgctcttctgtggaagccaataaattatatgcaattgatgaagcaattgctttggtaaaaaaagcagcgactgctaaatttgacgagtctgttgacgtatctttcaacttgggcgttgatccgcgtaaatctgaccaagttatccgtggttcggtcgttctgcctaaaggcaccggtaagataacccgtgtggctgtatttactcaaggtgcaaatgcagaagctgctaaagaagctggtgcagatatcgtcggtttcgaagatttggctgctgaaatcaaagcaggcaatctgaactttgatgtcgttattgcttctcccgatgcaatgcgtattgttggtcagttgggtactattttgggtcctcgaggcttgatgccaaaccctaaagtaggtacggttactcctaacgttgctgaagcagttaagaatgcaaaagcaggtcaagtacaataccgtacagataaagcaggtatcgttcatgcaacgattggtcgtgcttctttcgctgaagctgatttgaaagagaactttgatgcgttgctggatgctatcgttaaagccaagcctgctgccgctaaaggtcagtatctgaaaaaagttgctgtgtctagcaccatgggtttgggtattcgcgttgatacatcaagcgtaaataactaatcttaaggaattttcaagcagtttggttttctgggctgcttgaatttgggctacttaaaattaagtagatgtccaagaccgtagggatcgtaagatttaatcgtaactgccctacgcagacggtagtcctgaaacacattgcaagattgcttgtaagatgtctttttaggttaccgcgctggtgggatatcgttttggtatcctgtttataaacagtgggaggtagaccttgagtctcaatattgaaaccaagaaagtggcggtcgaggaaattagcgcggcaattgctaatgctcaaaccctcgtagtcgctgaatatcgcggtatcagtgtttccagtatgactgagcttcgtgcgaatgcacgtaaagaaggcgtttatttgcgcgttctgaaaaatactttggctcgtcgtgcagtgcaaggtacttcatttgcagaattggccgatcaaatggttggtccgttggtttacgctgcttctgaagatgctgttgctgctgctaaagtgttgcaccaattcgcgaaaaaagatgacaaaattgtcgttaaagccggttcttacaatggcgaagtaatgaatgctgctcaggttgctgagttggcttctattccgagccgcgaagagctgttgtccaaactgttgttcgttatgcaagctcctgtatcgggctttgcgcgcggtttggctgctttggcagagaaaaaagccggcgaagaagccgcttaatcgattttgtttctgttaatcaattattttttaatacaatatttggagtaaaatagcatggctattactaaagaagacattttggaagcagttggttctttgaccgtaatggaattgaacgacttggttaaagcttttgaagaaaaattcggtgtttctgctgctgctgttgcagttgcaggtcctgctggtgccggtgctgccgatgctgaagaaaaaaccgaatttgatgtcgttttggcttctgccggcgatcaaaaagtcggcgtgattaaagttgtccgtgcaattaccggtttgggtctgaaagaagctaaagacatcgttgacggcgcacctaaaaccattaaagagggtgtttctaaagctgaagccgaagacatccaaaaacaactggaagaagcaggcgctaaagtcgaaatcaaataatttgatgcttcttatgaaggctggcagttttctgccagccttattttgcttcttaaaataaacatcaagtattgtttacatttatttgcatagtttttatcaagtcattgcaaataaatgtaaatatcagattgatgcgtaccgttgtttcagacggcctattattgaaaattacttttcggagtgtgtatgaactattcgtttaccgagaaaaaacgtatccgtaagagttttgcaaagcgggaaaatgttttggaagttcctttcttgctagcaacccaaattgattcttatgcgaagtttttgcagctggaaaatgcttttgacaaacgtaccgatgacggtctgcaggcggcatttaattctattttcccgattgtgagccataacggttatgcgcgattggagtttgtgcattacacattgggcgagcctttgttcgatattcccgaatgtcagttgcgcggaatcacttatgcagcccccttgcgcgcgcgtatccgtttggtgattttggataaggaagcatctaaaccgacggtaaaagaagttcgtgaaaacgaagtgtatatgggcgaaattccgttgatgaccccgagcggttcttttgtgattaacggcacagagcgtgtgattgtctcccagttgcaccgttcgcccggcgtattcttcgagcatgacaaaggtaagacgcactcttccggcaaattgttattctccgcccgcatcattccctaccgtggttcatggttggattttgaatttgatccgaaagatttgctgtatttccgtatcgaccgccgccgtaaaatgccggtaacgattttgttgaaggctttaggctacaacaatgagcaaatcttggatattttctacgacaaagaaacgttctatttgtcttcaaacggtgttcaaaccgatttggttgcagaccgtctgaaaggcgaaactgccaaggtcgatatcttggataaagaaggcaatgtattggttgccaaaggtaagcgcattactgcgaaaaatatccgtgatattaccaatgcaggcctgacccgtttggatgtagaaccggaaagcctgctgggcaaagcattggctgccgatctgattgattcggaaaccggcgaggtattggcttctgccaatgatgaaattacagaagagttgttggccaaatttgatatcaacggcgtaaaagaaattacgaccctttatatcaatgagctggatcagggtgcttatatctccaataccttgcgtacggatgagactgccggccggcaggcggctcgtgttgcgatttaccgtatgatgcgtccgggcgaaccgcccaccgaagaggcggtcgagcaattgtttaaccgcttgttcttcagtgaagacagctacgatctgtcccgcgtaggccgtatgaaatttaatacgcgcacatacgaacaaaaactgtccgaagcccaacaaaactcttggtacggccgcctgctgaacgaaacgtttgccggtgctgccgacaaaggcggttatgtcctgagcgtcgaagatattgtcgcctcgattgcgactttggtcgagttgcgtaacggccatggcgaagtggacgatatcgatcacttgggcaaccgccgagtacgttcggtaggcgagctgactgaaaaccaattccgtagcggtttggcccgtgtggaacgtgccgtaaaagaacgtttgaatcaggcggaatcagaaaacttgatgccgcacgatttgattaatgcaaaacctgtttctgccgctattaaagaattcttcggctccagccaattgagtcagtttatggatcagaccaaccccttgtctgaagtaacccataaacgccgtgtatctgcattgggtccgggcggtttgacccgcgaacgtgcaggatttgaggtgcgggacgtgcatccgacccactacggtcgcgtatgtccgattgaaacgcctgaaggtccgaacatcggtttgatcaactcattgtccgtttatgcgcgcaccaatgattacggtttcttggaaacgccttaccgccgcgttatcgacggcaaagtaaccgaggaaatcgattacttgtctgccatcgaagaaggccgctatgtgattgcacaggcgaatgccgatttggattcagatggcaatctgattggcgatttggttacctgtcgtgaaaaaggcgaaaccattatggcaacgcccgaccgcgtccaatatatggacgtggcaactggtcaagtggtatccgttgcagcatccctgattccattcttggaacatgatgacgc

附录Bgaaccgcgcattgatgggtgccaacatgcaacgtcaggcagtgccttgcttgcgtcctgaaaaaccgatggtcggtaccggtatcgagcgttccgttgccgttgactctgctactgcaatcgttgcccgccgaggcggcgtggtcgagtatgtcgatgccaaccgcgttgtgatccgtgtccatgacgacgaagcgactgccggtgaagtgggtgtcgatatttacaacttggttaaattcacccgttccaaccagtctaccaatatcaatcagcgtcctgccgtcaaagccggcgatgttttgcaacgcggcgatttggtggccgacggcgcgtccaccgattttggcgaattggctttgggtcaaaatatgaccatcgccttcatgccgtggaacggttacaactacgaagactcgattctgatttccgaaaaagtggctgcggacgaccgctatacttcgattcacattgaggaattgaatgtcgttgcccgcgatactaagctgggtgcggaagacattacccgcgatattccgaacttgtccgagcgtatgcaaaaccgtttggacgaatccggtatcgtttacatcggtgcggaagtagaagccggcgatgtgttggtaggcaaggtaacgcctaaaggcgaaacccaactgacgccggaagaaaaactgctgcgcgccatcttcggtgaaaaagcatctgacgtaaaagatacttcattgcgtatgcctaccggcatgagcggtaccgttatcgacgttcaagtcttcactcgtgaaggtattcaacgcgacaaacgtgctcaatccattatcgattccgaattgaaacgctaccgtttggatttgaacgaccaattgcgtattttcgacaacgacgcattcgaccgtatcgagcgtatgattgtcggtcagaaagccaacggtggtccgatgaagctggccaaaggcagcgaaatcacgaccgaatatctggcgggtctgccgagcaggcacgattggttcgatatccgtctgaccgatgaagatttggccaagcagttggaactgattaaagtgagcctgcaacaaaaacgcgaagaagcggacgagttatacgaaatcaagaagaaaaaactgacccaaggcgacgaattgcaacccggcgtacaaaaaatggtgaaagtttttatcgccatcaaacgccgtctgcaagccggcgacaaaatggcgggccgccacggtaacaaaggcgtggtatcgcgcattctgccagtggaagacatgccttacatggcggacggccgtccggtagacatcgtactgaacccattgggcgtaccttcccgtatgaacatcggtcagattttggaagttcacttgggttgggcagcaaaaggtatcggcgagcgtatcgaccgtatgctgaaagagcaacgcaaagcaggcgagttgcgcgagttcttgaacagactctacaacggcagcggtaagaaagaagatttggatgccctgactgatgaagaaatcatcgaactggcctccaacctgcgcaaaggtgcatctttcgcctctcctgtattcgacggtgcgaaagagtctgaaatccgcgaaatgctgaacttggcttatccgagcgacgatcctgaggttgaaaaactgggcttcaacgacagtaaaacccaaatcacgctgtatgacggccgttcaggcgaagcatttgaccgcaaggttacagtaggtgtgatgcactatctgaaactgcaccacttggttgacgaaaaaatgcacgcgcgttctaccggtccgtacagtctggttacccagcagcctttgggcggtaaagcccagttcggcggccaacgtttcggcgagatggaggtttgggcattggaagcatacggcgcggcatacacgctgcaagagatgctgactgtgaagtctgacgacgtgaacggccgtaccaaaatgtacgaaaacatcgtcaaaggcgaacacaaaatcgatgccggtatgcccgagtccttcaacgtattggtcaaagagattcgctcactgggcttggatatcgatttggaacgttactaaacaaaagttttcagacggcctttcagggtcgtctgaaaaagtggtttcagaataagaatgaagcaatcggcatttaggccgtctgaaatcaaaagtaccgtttcccaatatcgaaaatccgccatgcggtaaaaatacttccttcaaggagcaaaaatgaatttgttgaacttatttaatccgttgcaaactgccggcatggaagaagagtttgatgccattaaaatcggtattgcctctcccgaaaccatccgctcatggtcttatggcgaagtcaaaaaacctgaaaccatcaactaccgtacgttcaaacctgagcgtgacggtttgttctgtgccaaaatctttggcccggtcaaagactacgaatgcttgtgcggaaaatacaaacgcttgaaatttaaaggcgtaacgtgtgaaaaatgcggcgtggaagtaaccctgtccaaagtgcgccgcgaacgcatgggtcatatcgaattggctgcgcccgtcgcacatatttggttcttaaaatccctgccttcccgcttgggtatggtgttagacatgactttgcgcgacatcgagcgcgtattgtactttgaagcatttgtggtaaccgatcccggtatgactccgctgcaacgccgccaattgctgactgaagacgattactacaacaagctggacgaatacggcgacgatttcgatgccaaaatgggtgcggaaggtatccgcgaattgctgcgtaccctgaatgtagcgggcgaaatcgaaatcctgcgccaagagttggaatcgaccggttccgacaccaaaatcaaaaaaatcgccaaacgcttgaaagtattggaagccttccatcgttccggtatgaaactggaatggatgattatggatgtgctgccggtattgccgcctgatttgcgtccgttggttccattggatggtggtcgttttgccacttccgatttgaacgatttgtaccgccgcgttattaaccgtaacaaccgtctgaaacgtctgttggaactgcatgcgcctgacatcatcgtccgcaacgaaaaacgtatgttgcaagaagcagttgactcgctgttggataacggccgtcgcggtaaagccatgaccggcgccaacaaacgcccgctgaaatcattggcagacatgattaaaggtaaaggcggtcgcttccgtcaaaacctgttgggcaaacgtgtggactactccggccgttccgtgattaccgtaggcccgtacctgcgtctgcaccaatgcggtttgccgaaaaaaatggctttggaactgttcaaaccgttcattttccacaaattggaaaaacaaggtttggcctctaccgttaaagcagcgaaaaaattggtagagcaagaagtaccggaagtatgggacatcttggaagaagtcatccgcgaacatccgattatgctgaaccgtgcgccgaccctgcaccgtttgggtattcaagcgttcgaacctatcttgattgaaggtaaagcgattcagttgcacccattggtgtgtgctgcgttcaacgccgactttgacggcgaccaaatggcggtacacgttccattgagcttggaagcacaaatggaagcacgcacgctgatgctggcttcaaacaacgtattgtctccggccaacggcgaaccgattatcgtaccttcccaagacatcgtattgggcctgtactatatgactcgcgatcgtatcaatgccaaaggcgaaggcagcctgtttgccgatgtgaaagaagtgcatcgcgcataccataccaaacaggtcgagctgggtacgaaaatcaccgtacgtctgcgcgaatgggtgaaaaacgaagcaggtgagtttgagcctgtcgttaaccgttacgaaacaaccgtcggccgtgcattgttgagcgaaatcctgccgaaaggcctgccgtttgaatatgtcaacaaagcgttgaagaaaaaagaaatttctaaactgattaacgcatcgttccgcctgtgcggcttgcgcgatacggttatctttgctgaccacctgatgtacaccggtttcggatttgcggcaaaaggcggtatttccattgccgttgacgatatggaaattccaaaagaaaaagcggccttgctggctgaagccaatgccgaggttaaagaaatcgaagaccaataccgtcaaggtttggttaccaacggcgaacgctacaacaaggtggtcgatatttggggtcgtgccggcgataagattgctaaagcgatgatggacaacttgtccaaacaaaaagttatcgaccgtgccggcaacgaagtcgatcaagagtcattcaactccatttatatgatggcggactccggtgcccgtggttctgcagctcagattaaacagttgtccggtatgcgtggcttgatggcaaaacctgacggctcgattattgaaacgccgattacctcaaacttccgtgaaggtctgaccgtattgcaatactttattgcgacccacggtgcgcgtaagggtttggcggataccgcattgaaaaccgcgaactccggttacctgactcgtcgtctggtagacgtaactcaagatttggtcgttgttgaagacgattgcggtacttcagacggctttgtcatgaaggcagtggtacaaggcggtgatgtgattgaagcattgcgcgatcgtattttgggtcgtgttaccgcgtctgacgttgtcgatccgtcaagtggcgaaaccttggttgaagccggtacgttgctgactgaaaaactggtggatatgatcgaccaatccggtgtcgatgaagtcaaagtccgtacgccgattacttgtaaaacccgtcacggcctgtgtgcacactgttacggtcgtgacttggcacgcggcaaactggttaacgccggtgaggcagtcggtgtgattgctgcacaatccattggcgaaccgggtacccagttgaccatgcgtacgttccacatcggtggtgcggcatcccgtgcggcagcagccagccaagtggaagccaaatccaacggtacggcacgattcagcagccagatgcgctacgttgccaacaacaaaggcgagttggttgtcatcggccgttcttgtgaagtcgtgattcacgacgatatcggccgtgaacgcgaacgccacaaagtaccttacggtgccatcctgctggtacaagacggtatggccattaaagccggtcaaaccttggcaacctgggatccgcatacccgtccgatgattaccgaacacgcaggtatggtgaaattcgaaaacgtggaagagggcgttaccgttgccaaacaaaccgatgatgtaaccggtttgtccactttggtggtgattgacggtaaacgtcgttcctctagtgcttccaaactgctgcgtccgactgtgaaactcttggacgaaaacggcgtggaaatctgtattcccggtacttctactccggtatccatggcattccccgttggtgcggtgattaccgtacgcgaaggtcaggaaatcggtaaaggcgacgtattggcgcgtattccgcaagcctcttccaaaacccgcgacattaccggcggcctgccgcgcgttgccgaattgtttgaagcacgcgtgccgaaagatgccggtatgttggcggaaattaccggtaccgtttccttcggcaaagagaccaaaggcaagcaacgtctgattgttactgacgtggacggtgtagcatacgagaccttgatttccaaagagaaacaaattctggtacacgacggtcaagtggtaaaccgcggtgaaaccatcgtggacggcgcggtcgatccgcacgatattctgcgtttgcaaggtatcgaagcactggcacgctacattgtccaagaggtgcaagaggtttaccgtctgcaaggtgtgaagatttctgataaacacatcgaagtcatcatccgtcaaatgttgcgccgtgtgaacattgcggatgccggcgaaaccgggttcattaccggagagcaggtcgaacgcggcgatgtgatggcggccaatgaaaaagctttggaagaaggcaaagaaccggcgcgttacgaaaacgtattgctgggtattaccaaagcttccctgtccaccgacagcttcatttctgccgcatcgttccaagaaacgacccgcgttctgaccgaagccgcgattatgggcaaacaagacgagttgcgtggtttgaaagaaaacgtcatcgtcggtcgcttgattcctgccggtaccggtttgacttaccaccgcagccgtcatcaacaatggcaagaggtggaacaggagactgccgaaacccaagtaacggatgaataatctttggtgcatccattcaataaaaaaccgcaagccttgagcttgcggtttttctttgtccgattaaggcaaaaacaagcgttttcgtcattttgaggcgtgtggattattccttaggtattttcgggccggagaccaacgaggtggcgggtgtcgtcggtacgtccggagaccaaaataactttgccagggatgttggtttcggcggtcaaaaaaagtagcgtcttaatgttttccatttaaacaaatgtcgtctg

附录Baaacttcagacggcatttcctttaagaaataaatatgaaacccagaaatctcttttttgcaggctgcctgctgacttcggcgacgtttgccgaggatatcggcgtacctgtcgaactgattaacgtcggtaatcggattgcgatgccgtctgaaggggaaagcctcgccctcctgccgtttgccgaggatgtaccgccggttcgcgatgcaatgccgtctgaagttcctaaaagcgcggcaggcggcgatgttcggggtgaccggatgagaatgccgattaacatcggatgagcgcggctttatggcataaaaaactgtcgtggaaaggatttacaccccaaataaatttccgttacaacaagatcaacagcaatatgcccgccttttattcgcgcagcggcaaggaacggtttgtcagtatagaaaaaacgtattgacagtattttcttcagtcgtccgactgattgtgagggatgtcggtaaatatttatcggcaaacaagaaaatcatctttcttcttgtcgttatgcttgactgtctgcttgcaataaaaatataattccactcttgccgacatggtgtcggcaagtatttaactcaacaggacgagaaaatatgccaactatcaaccaattagtacgcaaaggccgtcaaaagcccgtgtacgtaaacaaagtgcccgcactggaagcttgcccgcaaaaacgtggcgtgtgcacccgtgtatacacaactacccctaaaaaacctaactctgcattgcgtaaagtatgtaaagtccgcctgaccaacggttttgaagtcatttcatacatcggcggcgaaggtcacaacctgcaagagcacagtgtcgtattgattcgcggcggtcgtgtaaaagacttgccaggtgtgcgttaccacactgtacgcggttctttggatactgcaggtgttaaagaccgtaaacaagcccgttccaaatacggtgctaagcgtcctaaataattactgggacttaaataggcacgtcggccgcctaagctgaacaacggccgagtaagtgaatactcaattgggtattcatgggaatagacccgactgaatagattaaaggaaattaaaatgccaagacgtagagaagtccccaagcgcgacgtactgccagatcctaaattcggcagcgtcgagttgaccaaattcatgaacgtattgatgattgacggtaaaaaatccgttgccgagcgtatcgtttacggtgcgttggaacagattgagaaaaaaaccggcaaagtagcaatcgaagtatttaacgaagccattgcaaacgccaaacctatcgtggaagtgaaaagccgccgtgtaggtggtgcaaactaccaagttcctgttgaagttcgtccttcacgccgtttggctttggcaatgcgctgggttcgcgatgcggcccgcaaacgtggtgagaaatccatggacctgcgtttggcaggcgaattgattgatgcgtccgaaggccgtggcggtgcgttgaaaaaacgtgaagaagtacaccgtatggctgaagccaacaaagcattctctcacttccgtttctaattttgaaaggctaataaaatggctcgtaagaccccgatcagcctgtaccgtaacatcggtatttccgcccatattgacgcgggtaaaaccacgacgacagaacgtattttgttctataccggtttgacccacaagctgggcgaagtgcatgacggtgcggctactaccgactacatggaacaagagcaagagcgcggtattaccattacctccgctgccgttacttcctactggtccggtatggcgaaacaattccccgagcaccgcttcaacatcatcgacaccccgggacacgttgactttaccgtagaggtagagcgttctatgcgtgtattggacggcgcggtaatggtttactgcgcggtgggcggtgttcaaccccaatctgaaaccgtatggcggcaagccaacaaataccaagtgccgcgcttggcgtttgtcaataaaatggaccgtcagggtgccaacttcttccgtgttgtcgagcaaatgaaaacccgtttgcgcgcaaaccctgtacctatcgtcattccggttggtgcggaagacaacttcagcggtgtggttgatttgttgaaaatgaaatccatcatttggaatgaagtcgataaaggtacaacctttacctatggcgatattcctgccgaattggtcgaaactgccgaagaatggcgtcaaaatatgattgaagccgcagccgaagccagcgaagaactgatggacaaatacttaggcggcgacgagctgaccgaagaagaaatcgtaggcgcgttgcgtcaacgtactttggcaggcgaaattcagcctatgctgtgtggttctgcatttaaaaacaaaggtgttcaacgtatgttggacgcagttgtagaattgctgccagctcctaccgatattcctccggttcaaggtgtcaacccgaataccgaggaagccgacagccgtcaagccagcgatgaagagaaattctctgcattggcgttcaaaatgttgaacgacaaatacgtcggtcagctgacctttatccgcgtttactcaggcgtagtaaaatccggcgataccgtattgaactccgtaaaaggcactcgcgaacgtatcggtcgtttggtacaaatgactgccgcagaccgtactgaaatcgaagaagtacgcgccggcgacatcgcagccgctattggtctgaaagacgttactaccggtgaaaccttgtgtgcggaaagcgcgccgattatcttggaacgtatggaattccccgagccggtaatccatattgccgttgagccgaaaaccaaagccgaccaagagaaaatgggtatcgccctgaaccgcttggctaaagaagacccttctttccgtgtccgtacagacgaagaatccggtcaaaccattatttccggtatgggtgagctgcacttggaaattattgttgaccgtatgaaacgcgaattcggtgtggaagcaaatatcggtgcgcctcaagtggcttaccgtgaaactatccgcaaagccgttaaagccgaatacaaacatgcaaaacaatccggtggtaaaggtcaatacggtcacgttgtgattgaaatggaacctatggaaccgggtggtgaaggttacgagtttatcgatgaaattaaaggtggtgtgattcctcgcgaatttattccgtctgtcgataaaggtatccgcgatacgttgcctaacggtatcgttgccggctatcctgtagttgacgtacgtatccgtctggtattcggttcttaccatgatgtcgactcttcccaattggcatttgaattggctgcttctcaagcgtttaaagaaggtatgcgtcaagcatctcctgccctgcttgagccaatcatggcagttgaagtggaaaccccggaagaatacatgggcgacgtaatgggcgacttgaaccgccgtcgcggtgttgtattgggtatggatgatgacggtatcggcggtaaaaaagtccgtgccgaagtacctttggcagaaatgttcggttactcgaccgacctgcgttctgcaacccaaggccgcgctacttactctatggagttcaagaaatattctgaagctcctgcccacatagctgctgctgtaactgaagcccgtaaaggctaatcagaaaaggccgtctgaaactgaaaataaattttcagacggccattgttctttaatcgatctttatatgtaaaggaattagctcatggctaaggaaaaatttgaacgtagcaaaccgcacgtaaacgttggcaccatcggtcacgttgaccatggtaaaaccactctgactgctgctttgactactattttgtctaaaaaattcggtggcgctgcaaaagcttatgaccaaatcgacaacgctcctgaagaaaaagctcgtggtattaccattaatacctcacacgtagaatacgaaactgaaacccgtcactacgcacacgtagactgcccggggcacgccgactacgttaaaaacatgattaccggcgccgcacaaatggacggtgcaatcctggtatgttccgcagccgacggccctatgccgcaaacccgcgaacacatcctgctggcccgccaagtaggcgtaccttacatcatcgtgttcatgaacaaatgcgacatggtcgacgatgccgagctgttggaactggttgaaatggaaatccgcgacctgctgtccagctacgacttccccggcgatgactgcccgattgtacaaggttccgcactgaaagccttggaaggcgatgccgcttacgaagaaaaaatcttcgaactggctgccgcattggacagctacatcccgactcccgagcgagccgtggacaaaccgttcctgctgcctatcgaagacgtgttctccatttccggccgcggtacagtagtaaccggccgtgtagagcgcggtatcatccacgttggtgacgagattgaaatcgtcggtctgaaagaaacccaaaaaaccacttgtaccggtgttgaaatgttccgcaaactgctggacgaaggtcaggcgggcgacaacgtaggcgtattgctgcgcggtaccaaacgtgaagacgtggaacgcggtcaggtattggctaaaccgggtactatcactcctcacaccaaattcaaagcagaagtatacgtactgagcaaagaagagggtggtcgtcacactccgttcttcgccaactaccgtccgcaattctacttccgtaccaccgacgtaaccggcgcggttactttggaagaaggtgtggaaatggtaatgccgggtgaaaacgtaaccatcaccgtagaactgattgcgcctatcgctatggaagaaggcctgcgctttgcgattcgcgaaggcggccgtaccgtgggtgccggcgtggtttcttctgttatcgcttaattgaaggatattgataaatggcaaaccaaaaaatccgtatccgcctgaaagcttatgattacgccctgattgaccgttctgcacaagaaatcgttgaaactgcaaaacgtaccggtgcagttgtaaaaggcccgattcctttgccgaccaaaatcgagcgtttcaacattttgcgttctccgcacgtgaacaaaacttcccgtgagcaattggaaatccgcacccacttgcgcctgatggacatcgtggattggaccgataaaactaccgatgcgctgatgaagctggatttgccggccggtgttgatgtagaaatcaaagtccaataattcggactataaaaaatccccaagcaatcaatgcttggggattttttatgttatgccgagacctttgcaaaattccccaaaatcccctaaattcccaccaagacatttaggagcaccttcttccagcaaaccgcccaagccatgattgccaaacacatcgaccggttcccactattgaagttggaccgggtaattgattggcagccgatcgaacagtacctgaatcgtcaaagaacccgttaccttagagaccaccgcggccgtcccgcctatcccctgttgtccatgttcaaagccgtcctgctcggacaatggcacagcctctccgatcccgaactcgagcacagcctcatcacccgcatcgatttcaacctgttttgccgctttgacgaactgagcatccccgattacagtcatcaaccatattccggtttgtcggagaaagatgcatacgctgtgatgaccggataccgacccgttaaaagagtccgaccctatgccgtctgaaaattcaaaacgcttcagacggcatattgaagatatttctgatatttctgttgatatttctttgacttgtcagatataatgccgagcttggtacatttgtgccaagtttaactttgtctgaaagacaggccaatcgtagcctgtccctttactttaaaaggaaaataatcatgactttaggtctggttggacgcaaagttggtatgacccgcgtgttcgacgaacagggtgtttctgttccggtaaccgttttggatatgtctgccaaccgcgttacacaagtaaaatccaaagatactgacggctatactgccgttcaagttacctttggtcagaaaaaagccaatcgtgtcaacaaagccgaagccgggcactttgcaaaagcaggtgttgaagccggtcgcggtttgattgagtttgctttgactgaagaaaaactggctgaattgaaagctggtgacgaaatcaccgtttctatgtttgaagtcggtcaactggtcgatgtaaccggtacctctaaaggtaaaggtttctccggcacgattaaacgtcataacttcggtgcccaacgtacttcccacggtaactcccgttctcaccgtgttccaggctctatcggtatggcgcaagacccgggtcgcgtgttccccggtaaacgcatggccggccaatacggcaacaccaaagcaactgttcaaaaattggaagttgtccgtgttgacgcagaacgccaactgctgttggttaagggtgctgttccgggtgcggtcaacagcgatgttgtagttcgtcccagcgtgaaagtaggtgcgtaatggaattgaaagtaattgacgctaaaggacaagtttcag

附录Bgcagtctgtctgtttctgatgctttgttcgcccgcgaatacaatgaagcgttggttcatcagctggtaaatgcctacttggcaaacgcccgctccggtaaccgcgctcaaaaaacccgtgccgaagtaaaacactcaaccaaaaaaccatggcgtcaaaaaggtaccggccgtgcccgttccggtatgacttcttctccgctgtggcgtaaaggtggtcgcgcgttcccgaacaaacccgacgaaaacttcactcaaaaagtaaaccgcaaaatgtaccgtgccggtatggcgactattctgtcccaattgactcgtgacgagcgtttgtttgcgattgaggcgttgactgccgaaactcctaaaaccaaagtttttgccgaacaagtgaaaaatctgggtctggagcaagtgttgtttgtaaccaaacagctcgacgagaatgtttacttggcttcacgcaacttgccaaacgtgttggttttggaagctcaacaagttgatccttacagcttgctgcgttacaaaaaagtaatcatcactaaagatgcagttgcacaattagaggagcaatgggtatgaatcaacaacgtttgactcaagtgattttggcacctatcgtttctgaaaaaagcaacgtattggctgaaaaacgtaaccaaatgacgtttaaagttttggcaaatgcaaccaaacctgaaattaaagcggctgttgagctgctgttcggcgttcaagttgcagacgttactactgttaccattaaaggtaaagttaaacgttttggtcgcactttaggtcgtcgcagcgatgttaaaaaggcttatgtaagcttggctgccggtcaagagttggatttggaagccgctgctgcagctgcagataaggaataaacaaaatggcaatcgttaaaatgaagccgacctctgcaggccgtcgcggcatggttcgcgtggtaacagaaggtttgtacaaaggtgcaccttatgcacctctgctggaaaagaaaaattctactgccggtcgtaacaacaatggtcatattactacccgtcataaaggtggtggtcataaacatcattaccgcgtcgtagattttaaacgtaacaaagacggtatccctgcaaaagtagagcgtatcgaatatgaccctaaccgtactgcatttatcgcactgttgtgctatgcagatggtgagcgtcgctacattattgctcctcgtggtattcaagccggtgcagtattggtttccggtgctgaagctgcgatcaaagtaggtaacactctgccgatccgcaatattcctgttggtacaactattcactgtatcgaaatgaaaccaggtaaaggtgcgcaaattgcacgttctgccggtgcttctgcggtattgctggctaaagaaggcgcgtacgctcaagtccgcctgcgctctggcgaagtccgtaaaatcaacgtagattgccgtgcaaccatcggtgaagtcggtaacgaagagcaaagcctgaaaaaaatcggtaaagccggtgccaatcgttggcgcggtattcgtccgactgtacgtggtgttgtcatgaaccctgtcgatcacccgcatggtggtggtgaaggccgtacgggcgaggcccgcgaaccggtcagcccatggggtactcctgctaaaggctaccgcactcgtaataacaaacgcacggataacatgattgttcgtcgccgttactcaaataaaggttaatttagtatggctcgttcattgaaaaaaggcccatatgtagacctgcatttgctgaaaaaagtagatgctgctcgcgcaagcaacgacaaacgcccgattaaaacctggtctcgtcgttctaccattctgcctgattttatcggtctgaccattgctgtgcacaacggccgcacccatgtgcctgtgtttatcagcgacaatatggttggtcataaattaggcgaattctcattgacccgtacctttaaaggccacttggccgataaaaaggctaaaaagaaataaggtgaatcatgagagtaaatgcacaacataaaaatgcccgtatctctgctcaaaaggctcgtttggtagctgatttgattcgtggtaaagacgttgcccaagctttgaatattttggctttcagtcctaaaaaaggtgccgagctgattaaaaaagtattggagtcagctattgctaatgccgagcacaataacggtgcggacattgatgaactgaaagtggtaactatctttgttgacaaaggcccaagcttgaaacgttttcaagctcgcgccaaaggtcgcggtaaccgcatcgaaaaacaaacttgtcatatcaatgtgacagtgggtaactaaggaaaagctatgggacaaaagattaaccctacaggctttcgcctggcggtaactaaagactgggcttcaaaatggtttgctaaaagcaccgacttttctactgttttgaagcaggatatcgatgttcgcaattatttgcgtcaaaaattggccaatgcttcggttggtcgagtggttattgaacgccctgcaaaatctgcacgcattaccattcactccgctcgtccgggtgtggttatcggtaaaaaaggtgaggatatcgaggttttgaaacgtgacttgcaagtcttgatgggtgtacctgttcatgtaaatattgaagagattcgccgtcctgagttggatgctcaaattattgctgacggtattgcccagcagttggaaaagcgcgttcaattccgtcgtgctatgaaacgagcaatgcaaaatgcaatgcgttctggtgctaaaggcattaagattatgacttcaggccgtctgaatggtgcggatattgcccgtagcgaatggtatcgtgaaggtcgcgtgccactgcatactttacgtgcaaatgtagattatgcaaccagcgaagcgcacaccacatatggtgtattgggtctgaaagtttgggtttatacggaaggcaatattaaatcttccaaacctgaacatgagagtaaacaaagaaaggcaggtagacgtaatgctgcagccaactagactgaaataccgtaagcaacaaaagggtcgcaataccggcatcgctactcgcggtaataaggtaagtttcggtgagttcggcttgaaagccgtaggtcgtggtcgtttgactgcccgtcaaatcgaagctgctcgtcgtgcaatgacccgtcatatcaaacgtggtggtcgtatttggattcgtgtattccctgataaaccgattactgaaaagcctattcaagttcgtatgggtggcggtaaaggtaacgtggaatattacattgccgaaattaaaccaggtaaagtgttgtatgaaatggatggcgttccagaggaactggctcgtgaagcattcgagttggctgctgccaaattgcctattcctacaacctttgtagtaagacaggtgggtcaataatgaaagcaaatgaattgaaagacaaatccgttgagcagttgaatgcagatttgttggacttgttgaaagctcagtttggcttacgtatgcaaaacgctaccggtcaattaggcaaaccaagtgaattgaaacgtgtacgtcgcgatattgctcgtattaaaaccgttttaactgaaaaaggtgctaagtaatgagcgaaactaaaaatgttcgtactttgcaaggcaaagtagtaagcgacaaaatggataaaaccgtaacagtattggttgagcgtaaagtaaaacatccgctgtatggtaagattattcgattatctactaaaatccatgcccatgatgaaaataatcaatatggaattggtgatgtggttgttatatcggaatcccgtccattgtcaaaaactaaatcttgggttgtcagtgagctggttgagaaagcacgttctatttaagaattaaagcaacgtgcttggaatgggaaacgaagtattgcagcaaatttaatttgcgtgtaaacttcgtttcctgtctttcagtttcttctggaagtttcttccctttcggggtccaagactggtttacttgaaccgcaaggtttcatttaataagcagcggctttgctgtaagttatctgaaagtggtaaattaagttggttaatttaaaggtaataacatgattcaaatgcagaccatcttagatgtggctgataactctggtgcgcgtcgcgtaatgtgtatcaaggtattgggcggatctaagcgtcgctacgcttctgttggcgatattattaaagtggcagttaaagatgcggctccgcgtggccgtgtcaaaaaaggcgatgtatataatgcggtagttgttcgtactgctaagggtgtacgtcgtcctgatggtgcgttaattaaattcgataacaatgccgccgtgttactgaataataaacttgaacctttgggtactcgtatctttggtccggtaacccgtgaattgcgtactgagcgatttatgaaaatcgtttcattggcacctgaagtattataaggaatggcacgatgaataaaatcattaaaggcgatagggttgtagtaattgctggtaaggataaaggtaagcagggtcaagtagttcgagtgttgggtgataaagttgttgttgagggcgttaatgttgtaaaacgccatcaaaaacctaatccaatgcgtggcattgagggcggtattattactaaagaaatgcctttggatatttctaatatcgcaatcctgaatccggaaactaataaagcggaccgtgttggtattaagctgattgaaaatgaaggcaaagttaaacgcgttcgtttcttcaaatcaaatggctctatcattggggcataaggagataacatggctcggttgagagagttttataaagagacagttgttcctgaattggttaaacaatttggttacaaatcagtaatggaagtcccgcgtattgaaaaaattaccttgaatatgggtgtgggtgaggctgttgctgataaaaaagttatggaacatgctgtttccgatttagagaaaattgccggtcaaaaaccggttgttactgttgcccgtaaatctatcgcaggttttaaaatccgtgataactatccggttggttgcaaagtaacattgcgtcgtgatcaaatgtttgaattcttggatcgtttgattactattgcattacctcgcgtacgtgacttccgtggtgtgagcggtaaatcatttgatggccgtggcaattacaatatgggtgttcgtgagcaaattatttttccggaaattgaatacgataaaattgatgctttgcgtggtttgaatattactattactactacagcaaaaaccgatgaggaagcgaaagctttattgtcattgtttaaatttccgttcaaaggataatcatggctaagaaagcacttattaatcgtgatctgaaacgtcaagctttggctaaaaaatatgcggctaaacgcgcggcaattaaagcggtaatcaatgattcgaatgcaactgaggaagagcgttttgaggctcgtttgaggtttcaatccattcctcgtaatgcggcacctgtgcgtcaacgtcgtcgttgtgctttgacaggtcgccctcgtggtactttccgtaaatttggtttgggtcgtattaaaatccgtgaaatcgccatgcgtggcgaaattccgggtgttgttaaagccagctggtaataggagtaattaagaatgagtatgcatgatcctatttccgatatgttgactcgtatccgcaatgcgcaacgtgctaataaagcagcggttgcaatgccttcttcaaaattaaagtgtgctattgcaaaggtattgaaagaagaaggatatattgaggacttcgcagtttcatctgacgtaaagtctatattggaaattcaattaaaatactatgcaggtcgtcctgtaattgaacaaatcaagcgtgtatctcgccccggtttgcgtatttataaagcgtctagtgagattccaagtgttatgaatggcttgggtattgctattgttagtacttctaaaggtgtaatgactgatcgtaaagcacgttctcaaggtgttggtggtgagttgttatgcattgtagcctagtggaggaaaagaaatgtcacgtgtcgcaaaaaacccagtgactgttcccgctggtgtagaagtaaaatttggagcagaggcattagttattaagggtaagaacggtgaattgtcttttcctttgcattctgatgtagccattgaatttaatgatggcaaattgacttttgttgcgaataacagcagtaaacaagcaaatgcaatgtctggtactgctcgcgcattagtcagcaatatggttaaaggtgtttcagaaggttttgagaaaagattgcaattgataggtgtgggttatcgtgctcaagcacaaggtaaaatcttgaatctgtctttgggtttttctcatccgatcgtatatgaaatgcctgaaggtgtctccgttcaaactcctagccaaacagagattgttttaaccggctcggataaacaagttgttggtcaagttgctgctgagattcgtgcgttccgtgctcctgagccttataaaggtaaaggtgttcgctatgtaggagaagtagtggtaatgaaagaagccaagaaaaaataattgaggttcacta

附录Batggataaacatacaacccgactccgtcgtgcacgcaaaacccgtgctcgtattgcggacttgaaaatggtaagattatgtgtgttccgaagcaataatcatatttatgctcaagtaattagtgctgaaggtgataaagtattggctcaagcctctacattggaagctgaggtgcgcggtagtctgaaatctggaagcaatgttgaagcagctgcaatagttggtaaacgtatcgctgaaaaagctaaagcagcaggtgtagaaaaggttgcttttgatcgttcaggtttccaatatcacggtcgtgtgaaggctttggctgaagctgctcgtgaaaatggtttaagcttctaaatatttggagactttcagatggcaaaacatgaaattgaagaacgcggtgacggtctgattgaaaagatggtcgctgttaatcgcgtaactaaagtagttaaaggtggccgtatcatggctttctcagcactgactgttgttggtgatggtgatggtcgcattggtatgggcaaaggtaaatcaaaagaagtaccagttgctgttcaaaaagcaatggatcaagctcgacgctctatgattaaagtacctttgaaaaacggtactattcatcatgaggttattggccgtcatggtgctactaaagtatttatgcagcctgctaaagagggtagtggcgtaaaagccggtggacctatgcgtttggtttttgatgctatgggcattcataatatctccgccaaagtgcacggatctactaacccatataatatcgtacgtgcaacattagatggtttgtctaagttgcatactcctgctgatatcgcagccaaacgtggcttgacagtggaagacattttgggagttaaccatggctgaacaaaaaaagattagggttacattggttaaaagcctgattggtacaattgaatctcatcgtgcatgtgcacgcggtttaggtttgcgtcgtcgcgagcatacggtagaggttttagatacccctgaaaaccgtggtatgattaataaaatcagctacttgttgaaagtggagtcttgatatgtttttgaatacaattcaacctgctgttggtgctacgcatgctggtcgtcgtgttggacgcggtattggtagtggtcttggcaaaacgggtggtcgtggtcataaaggtcaaaagagccggtctggtgggtttcataaggtgggtttcgagggtggtcaaatgcccttgcaacgacgcctccctaaaagaggttttaaatctttaacagcatcagctaatgcacagcttcgtttaagtgaactggaatcaattgctgttaatgagattgatattttggtcttaaagcaagcgggtctgattgcatctacagtctctaatgttaaagttattgcttctggtgaaatttctaaggcagttgctttgaagggtattaaagttaccaaaggtgcgagagctgctatcgaggctgttggtggtaagattgaaatgtaaggtttaatattgtggctaatcaacaaacgtcatcaggttcatccaaatttggagatcttaagaaacgtcttttgtttctatttggagcattgattgtttttcgaattggtgcccatatacccgtacctggagttgatgctgttgctttagctaaattatacgaaagcgctggaaacggcatcctgggaatattgaatatgttttccggtgggtcgttagagcgctttagtatatttgcaataggaattatgccatatatttcagcttctattattgtacagctcgcttctgaaattttgccatcattgaaggctttaaaaaaagaaggggaggctggtagaaaggtaattacgaaatatactaggtatggtactgttttgttagcaattcttcaaagtctaggtgttgcatctttcgtatttcagcaaggaattgttgtaacaagttcatttgagtttcatgtttccacggtagtttctttggtaacgggaaccatgtttcttatgtggcttggggagcaaattactgaaaggggtatcgggaacggtatttctttaatcattacggcaggtattgcttcaggtattccttcgggtattgcaaagctggttacactgacgaaccaaggttctatgagcatgcttacggcgttgtttattgtatttggtgccttattattaatttatttggttgtatactttgaaagtgcacagcggaagattcctattcattatgcaaaacgccagtttaatggtagggcgggtagtcaaaatacgcatatgcctttcaagttgaatatggctggtgttattcccccaatttttgcttccagtattattctatttccatctactcttttaggttggtttggttcggctgatacaaatagtgttttgcacaaaatagctggattgttacaacacggtcaattgctgtatatggctttatttgcagcgacagttattttcttttgttatttttatacggctttggtttttagccctaaagaaatggcagagaatttaaaaaagagtggtgcttttgttcctgggattagacctggtgagcagacctctaggtatttagaaaaagttgtattacgtttgacattgtttggagctctttatattacaactatttgtttaattccagagttcttaactacggttttaaatgtacctttttatttgggtggcacgtctttgttgattctagttgttgtaacgatggattttagtacacaaataaattcgtataggcttactcaacagtatgataagttaatgactcgttcagaaatgaaatcattttctcggaaatagaattatggcgaaagaagatactatccaaatgcaaggtgaaattcttgaaactttacctaatgcaacatttaaagtaaaacttgagaatgaccatattgtattgggtcatatttctgggaagatgcggatgcattacattcgtatttctccgggagataaggtcacagtagagctgacaccttatgatctaactagggctcgaatcgttttcagagcaagataaaccaataaaaggaaaataaaatgcgtgtacaaccatctgttaagaaaatttgccgaaattgcaagattattcgtcgaaatcgtgtagttcgtgtaatttgtactgatctccgtcacaaacagcgtcaaggttaatggaatatttcttttaatgtgattctgtgatatagtgacacactttgccctaaaaaggaaaaaatatggctcgtattgcaggggtaaatatccctaataacgcacacatcgtaattggtcttcaggctatttacggtattggtgctactcgtgctaaattgatttgtgaggctgcaaatattgcgcctgatactaaagcaaaagatttggacgagactcaattagatgctttgcgtgaccaagttgccaagtatgaagtagaaggtgatttgcgtcgtgaggtaactatgagtatcaagcgattgatggacatgggctgctatcgtggcttccgtcatcgtcgcggcttaccatgccgcggtcaacgcactcgtacaaatgcgcgtacccgcaaaggtccgcgtaaagcgattgctggtaagaaataaattttaaggaattttattaatggctaaagcaaacacagcttcacgtgtacgtaaaaaagtacgtaaaaccgtgagtgagggtattgtgcacgttcatgcatctttcaacaataccatcattacaatcactgaccgtcaaggcaatgcgttgtcttgggctacctctggcggcgctggttttaaaggttctcgtaaaagtacaccatttgcagcacaagttgcagcagaagcagctggtaaagttgcccaagagtatggcgttaaaaatttagaggttcgtattaaaggtccaggtccaggtcgtgaatcctctgtacgtgctttgaatgctcttggtttcaagattaccagcattactgacgttaccccgttgcctcataacggttgccgtccgcctaaaaaacgtcgtatttaatattggagtgatttgaaacatggcacgttatattggccctaaatgtaagttggcacgtcgcgaaggtacggatttgtttttgaagagtgcgcgccgctctttggattctaaatgtaaaattgattccgctcctggtcagcatggtgcaaaaaaaccgcgtttgtcagactatggtttgcagttgcgtgaaaaacaaaaaatccgccgtatttatggcgtattagaacgtcagttccgtcgttatttcgcagaagctgatcgtcgtaaaggttctaccggcgagttgctgttgcagttgctggaatctcgtttggataatgtcgtttatcgtatgggtttcggttctacccgagctgaagcaagacagcttgtttctcataaggcgatagttgtgaatggacaagttgtcaatattccttctttccaagtgaaagctggtgatgttgtctcagttcgtgaaaaagccaaaaaacaggtacgtattcaagaagcattgggtttggcaactcaaatcggcttgccgggttgggtttctgtagatgcggataaacttgagggtgtgttcaaaaacatgccggatcgctcggaattgaccggtgatattaatgaacagctggtggtagagttctactctaaataatgctagctcagtgagggacagttaaatgcagaatagcacaaccgaatttttgaaacctcgtcaaattgatgtaaatactttttctgcaactcgtgcaaaagtatctatgcagccatttgaacgtggtttcggtcataccttaggtaatgctttgcgccgtatcttactgtcatccatgaatggttttgctcctactgaagtagctattgccggtgtattacacgaatattctactgttgatggtattcaggaagatgttgttgacattttgctgaatattaaaggtattgtgtttaaactccatggtcgtagccaagttcaacttgtgttgaagaaatcaggttcaggtgtcgtatctgccggtgatattgagttgccgcatgatgtagaaattctgaatcctggtcatgtcatttgtcatttggctgataacggtcaaattgagatggaaattaaagtagagcaaggtcgtggttatcaatctgtttcaggtcgtcaggtagttcgtgatgagaaccgtcagattggtgcaatccagttggatgcgagcttttcgcccatcagccgtgttagctttgaggttgaacctgcacgtgtagagcagcggacggatcttgataagttggttttggatatcgaaaccgacggttctattgatcctgaggaagctgtacgcagtgcggcacgtattttgattgatcagatgtctatttttgctgatttgcagggtacgcctgtggaggaggttgaagaaaaagcacctcctatcgaccctgttcttttgcgtccggtggatgatctggaattgacagtacgttcagctaattgtttgaaagctgaggatatttattatattggcgatttgattcaacgcactgaaaccgagcttcttaaaacgccgaatttgggacgtaaatctttgaatgagattaaggaagtattggcatctaaaggtttgacactgggttctaagttggaagcatggccacctgtaggcttggaaaagccttaatgaagaattaaaggataattgatatgcgtcatcgtaatggcaatcgcaaattaaaccgtaccagcagtcatcgtgctgcaatgctgcgtaatatggcgaattcattattgactcacgaagctattgtaacaactctgcctaaggccaaggaattgcgccgtgtagtagagccgttgattacattgggtaaaaagccgtcattggcaaaccgccgtttggcatttgaccgtactcgcgaccgtgatgttgtagtaaaactgtttggcgatttgggtcctcgttttactgctcgtaacggtggttatgttcgggtgttgaaatacggattccgtaaaggtgataatgcacctctggcactggttgaattggttgacaaaccggctgctgagtaattttagtcatataacgccatctgccgaaaagcaggtggcgttatttttgcaatatctgataggtaatagggtattggctatcatgtttaaaatattaattgaatagctaaggtttgcgcggtaaacttacatcattaaaaaattctatgatggtttatataatgaatgctttcgatataaagtcgacaaagatggacgtattgtctatatctttgcatacgtcagacttgtttgatttggaagatgtgctggtcaaattgggcaagaagtttcaagagtctggtgttgttccatttgtgctggatgttcaagagtttgattatcccgagtctttggatcttgctgcattggtttcgttgttttcaaggcatggtatgcaaattttgggtctgaagcattctaatgaacgttgggctgctgcggctatgaagtatcatttgctgttttgtctgtctcattcggaaaatgttaaagaactgggtcaggttgaggtgcagaaaacggaggatggtcagaaagcaaggaaaacagtattgattacatcccctgtccgtaccggtcagcaggtttatgccgaagatggcgattt

附录Bgattgttacgggggcggtcagccagggggcggaattgattgcggatggcaatatacatatttatgcgccgatgagggggcgtgctttggccggtgccaagggtgatacttctgcccgcatatttatccactccatgcaggcagaactggtttctgtggcgggtatttaccgtaattttgaacaggatttgccgaaccatctgcacaagcagccggtacagatattgttgcaggataaccgattggttatcagtgcaattggctcagagtaattgtttgatatttaaaaaggaaatattgtggcaaaaattattgtagtaacttcaggtaagggcggtgtcggtaaaacgactaccagtgccagtattgcgacaggtttggcattacgcggatataaaactgcggtaattgattttgatgtgggtttgcgtaacctcgacctcattatgggttgcgagcgtcgtgtcgtttatgacctgatcaatgtcattcagggggaggcgacgctcaaccaagctttgattaaagataaaaattgtgaaaacctgtttattttgccggcttcccagactcgggataaagacgctttgacacgcgagggcgtagaaaaagtgatgcaggagctgtccggcaagaaaatgggctttgagtatattatttgcgactctcctgccggtattgagcagggtgcattgatggcgttgtattttgctgatgaagccattgtaacgaccaatcctgaggtttccagtgtgcgtgactccgacaggattttgggaattttgcaaagcaaatcccataaggcagagcaaggcggttcggttaaagaacatctgttgattacgcgttattctcccgaacgtgtggcaaaaggcgaaatgctgtctgtacaggatatttgcgatattctgcatattcctttgctgggtgtgattcctgaatcccaaaacgtcttgcaggcatccaattccggagaaccggtcatccatcaggacagcgtggcggcttccgaggcatataaggacgttattgcccgtcttttgggcgagaaccgtgaaatgcgtttcttggaagctgagaaaaaaagcttcttcaaacgtctgtttggaggataaggtatgtcattaatcgaatttttattcggcagaaagcagaaaacggcaaccgttgcccgcgaccgccttcaaatcatcattgcccaagagcgcgcccaagaaggtcaggctccggattacctgccgactttacgtaaagagttgatggaagtcctgtccaaatatgtgaatgtttcattagacaatatccgtatttcccaagaaaagcaggatggtatggatgtgcttgagttgaacattactttgccggaacagaaaaaggtataggacatgaccttaaccgaattgcggtacatcgtcgcagtcgcccaagaacgtcatttcggcagggcggcgcggcgttgttttgtcagccagcccactttgtctattgccattaagaaattggaagaagagcttgccgtctctttgtttgaccggagcagtaacgatattattacgaccgaggcgggggaacgtatcgttgcacaggcgcgtaaggtattggaagaggcggagcttatcaggcatttggcaaatgaagaacaaaacgagctggagggtgcgttcaaactcgggctgatttttacggttgcgccgtacctgctgccgaaactgattgtttcgttgcgccgtactgcaccgaaaatgcctttgatgttggaagagaattacacgcatactttgaccgagtcgctcaaacgcggggacgttgatgcgattatcgttgccgaaccgtttcaagagccgggcattgttaccgaacccttgtatgacgaaccgtttttcgtgattgtcccgaaagggcattcatttgaggaactggatgccgtttcgccccggatgctgggtgaggagcaggttttgctgctgacggaaggcaactgtatgcgggatcaggtactctcaagctgttccgaattggcggcgaaacaacgtatacaggggttgaccaatacattgcagggcagctcgattaatacaatccgccatatggttgccagcggtttggcaatcagcgtgttgccggcaaccgcactgaccgaaaacgatcatatgctgttcagcattattccgtttgagggtacgccgccaagccggcgggtcgtattggcgtaccgccgcaattttgtccgtccgaaggcgttgtcggcgatgaaggcggcgattatgcagtcgcagcttcacggggtaagttttatctgcgactaggcgcaggcattgttttcaaaacgccatttccctgagccgacaacacggtatgccaagatattgccgtcatcatcgattttgagtatagcatcgccacggaaactgccgtcctgaagatattcgacttttgcatcactgtgaatgttttcatcagtgccgatgcaatgccatgtatagtggattaacaaaaaccagtacggcgttgcctcgccttgccgtactatttgtactgtctgcggcttcgtcgccttgtcctgatttttgttaatccactataaaagaggccgtctgaaaaacatttttcagacggccttgtttattcaatcaaatcagtctttcaacttcgccaactgattttgaacttttgccattttgtcttccaattccgccaaatcggctttgtctttttccaccagatgcgcaggggctttttcggtgtagccgggtttggagagtttggcgttgagtttgtccaaggctttttgcagcttctcggcttctttgctcaaacgggcggtttcggcggctttgtcgatttcgactttcaacatcaggcgcgcgccgttgcagacggcgacgggcgcgtcttcgctttcgggtagggcggcgacttgctgtgcttcggtcaggcgggtcatcatcggcaggtatttgaggtagtccgccaagtcgtccgtgctttcgacaaacagcggggcttttacgttgggctggatgcccatttcgccgcgcaggttgcggactgcgccaatcaaatcctgcaacacggtcatttgctcgaatgccgtctgaacaatctcgccgctgtcggcttcggggaagcgggcgagcatgatgctgtcggcggttttcgcgtcgcacataggagcgacggtttgccacagttcttcggtgatgaacgggataatcgggtgcagcaggcgcagggcggcttcgagtacgcgcaataaggtatggcgtgtggcgcgttggcggctggcgcagccggtttgaagctgcactttggcgagttccaaataccagtcgcaatagtcgttccatacgaagctgtacagggtttccgccgccaaatcaaagcggtaggtttcgtaggcttgcgtaacctgttcgatggtctgattcagacggcctacaatccacatatcggggaaggagtagccgcgcggttcggcagcggttgcgccgtaaccgcagtcttggttttcggtgttcatcaagacgaagttggtggcgttccagattttgttgcagaagttgcggtagccttcggcgcgtttgaagtcgaagttgaccgaacgccccaagctggcgtagctcgccatagtgaagcgcaaagcgtccgcgcccatactcggaatgccttcggggaagagttttttcgtggcttcttccactttcggcgcggtttcgggtttgcgcaggccggtggtgcgttttaccagcagtttttccaagccgatgccgtcgatcaaatccacagggtcaatgacgttgccttcggatttggacatttttttgccttcgtggtcgcgcacgatgccgtggatgtacacgtctttaaacggtactttgccggtgaagtgggtggtcatcataatcatacgcgccacccagaagaagatgatttcgtagccggttactaagacattggacggcaggaaggctttgagttcgtcggtttcagacggccagccgagtgtggagaacggcacaagcgcggaggagaaccatgtatccaatacgtcttcttcgcgagtcaagcctgttttgccggcttgtttttcggcttcttcctgattgcgggcaacatacacattgccttcgttgtcgtaccatgcagggatttgatggccccaccacagttggcgtgagatacaccagtcttggatgttgttcatccattggttgtaagtgttgacccagttttcagggataaagcgtaccgcgccgctatcaacggcttttttggctttatcggcgaggctcaagcctttgaactcgctgtccggctcgccgccgtttggggtggcggacatggcgacaaaccattggctggtcagcataggttcaatcaccgaacctgtacggtcgcctttcggcgtcatcagcgtgtgtggtttgatttcgaccaagaaaccttgttcctgcaaatcggcaaccatttgtttgcgcgcggcaaagcggtctaagcctgcgtatttttcaggcagggcaaagcctagttgcgcttcgcctttgaagttgaacacttcggcgtttgccagcactttggcttccaagttgaacacattaatcaggcgcgtgtcgtggcgtttgccgacttcgtagtcgttgaagtcgtgtgcaggcgtgattttcacgcagcctgtgccgaagtctttttcaacgtattcgtcggcaatcacggggatagtacggccggtcagcggcaggattaattccttgccgattaagtgggtataacgttcgtcttcaggattgacggcaacggcaacgtcgcccagcagcgtttcaggacgggtggtcgccacgataacggcttcggcgggattgtccgccagcggatagcggatgtgccacatagagccttgttcttccacgctttccacttccaaatccgataccgccgtgccaagcacgggatcccagttcaccaagcgtttgccgcggtaaatcaagccttgctcatacaggcgcacgaacacttcggttacggtttcggcgcgcacgtcgtccatcgtgaaatactcgcgcgtccagtcggcagagcagcccacgcggcgcatttgttgggtaatcgtgccgccggaaacttctttccattcccacactttctccaaaaatttttcgcgacccaagtcatggcgggacacgttttgcgcagcaagctgacgctcaaccacaatctgcgtggcgatgcccgcgtggtctgtgccgggaatccaggcggtgttgcagcctttcatgcggtagtagcgggtcagaccgtccataatggtttggttgaaggcatgacccatgtgcagcgtgccggttacgttgggcggcggcagttggatggagaaagacggtttcgtcaaatccatatcaggttggaaatagccctgctcttcccagttttgataatgtttggattcgatttcggctggattgtatttgtctaacatgatggaactttgtgaaattaaggttatttttgatgtgcggattataacgcaaaaaggccgtctgaatcatttcagacggcctttggcatacaggttttaaaaatggaacaataccaggctgacggcaatcaccgccatacccgttgtcaggccgtaaacggtttcatggccgtctgaatagcgtttggcagccggcagcagctcgtccaacgccaaaaacaccatcacaccggctatcacgccgaataccgaaccaaacacggcaggcgacaaaaacggctgcaaaaccaaatagcccaaagccgcccccaacggctcggccaagccggatagcagacacgcccacaccgttttcttacggctgcgggtggcaaaataaaccggcgcggcgatggaaatgccctccggaatattatggatggcaatcgccaaggccaaaggcatcccgactgctggattttccaatgtggcaaaaaacgtcgccaagccttcggggaaattgtgcgcagtaatcgcaaacgccgccatcatgccgactcgcgcgatatggcggcgtttgctttcttgaaacgacgggtcttgcgcgtctaaagtttcatgcgggttcggcaccagacggtcaatcagcgcaatgccgcccatcccggccaaaaatgccatggtcgccgccgcaaacgcgtggtctttatcataaatttcagcgaacgcctcgctggacttactgaaaatctccgtcagggaaacatataccatcgcaccgccggcaaacgccaaaccaaacgacaacacacgcggattgggcgttttggaaaacatcaccaagccactgcctaatacggtaaacaaaccggcagccaatgtgatggaaaaggcaacggccaaattggacatcgaaaaatcgggcatgagaaaacctgcgctaaaagctgggacaggttcagactaacactttttaatgtatatgataatagttattatttattttattgattggatacacggattttgaaacaaaaggccgtctgaaaaatgattttcagacggcctttaaatttgaaatgccgctaaaccttagtgctttccagcttaagcctgataacgcgacaggctcaaatcgtcgctgcggatttcggtgtctttgccgctcacgatatcggcggttaattttgccgaacccagcgacatggt

附录Bccagcctaaagtaccgtggccggtattcagaaacaggttgtcaaagcgggtgcgaccgattaacggcgtgctgtcgggcgtcatcggtctgaggccgctccagaacgatgcttggctcaaatcgccgccttccgggaacaagtcgttgacgaccaaagccaaggtttcgcggcgtttttcgggcagtttgatttcgtagcccgacaattccgccataccgccgacgcggattctgttgtcaaagcgcgtgatggcgactttgtagctttcatctaaaacggtggacaccggtgcgccgtctgaattggtgaccggcagggtcaaggaatagcctttgacgggataaatgggcagattgagatccaactgcgccaaaaccgtcctgctgaagcaaccgagcgcgcagacaacggcatctgcttcaaaccgccctgtttcggtttcaacggttttgatgcgcagcccgttgtggtcgatgcggctgatgttttggttgaaatgaaaccgtacgcccttttcctgacacaatttgtataggttttcagtgaagaggcggcagtcgccggtcgcatctgcaggcaggtgcaggccgccggcaattttggcggtaacgcgtgccagcgcaggctcaaattctgcacattcttcgggtttcagacggcggtacggcacgccgtagcgttccaaaacggcaatgtcttgttttgccgcttcgacttctttggtttggcggaaaatctgcaacgtcccttttttgcgtccctcaaaattcatgccggtttgcgcttcaaaacggcggaacatttcacggctgtattcggaaatcctgaccatgcgctctttattggtttgatagtgcgctgccgtgcagttttgcagcatttgccacagccattcgatttgatacaggctgccgtcggggcgaaacagcaaaggcggatggcttttaaacagccatttcagcgctttggtcgggataccgggtgcagcccaaggcgtggtatagccgtaagaaagctggcctgcgttggcaaaactggtttccatcgccacaccctcggcgcggtcgatgaccgttacttcatgtccggcctctgccagataccacgcggaagacacgccggcaacacccgcacctaaaacaagcactttcatgtttctccctccggctttttcaaaacagacttaatatgccgtgccgtctgaatattcggattcagacggcctcggatattaatgcggcaattcgccgtttgtgattttttgtttgaagtcgcgcgtttcattgacgatgactttcgccatcaataaaagtgcaatcaggttgggcaatgccatcaagccgttgaatgtgtccgaagccagccacaccaaatcaaggctcaacacggtacccagcataacggaagaaacataacccacgcggtacaaaccggcaaatttctcgccgaaaacatacaccgcgcatttttcgccgtaatagcaccagcccaaaatggttgagtaggcaaagaaaatcaggccgatggtaacaatccagccgccgatgccgggcagcattttttggaatgtgacggttgtcagtgccgcgccgctcacttcaggtttgacaaactcgccgcccgcgccgagcagtcccattaccaacacgatgccggtaatcgagcaaacgacgatggtatccaaaaacgtaccggtcatagaaaccaaggcctgacggacgggatggtcggttttcgcggctgcggcggcaataggcgcagaacccatacccgcctcattggagaacacgccgcgcgccacgccgtagcggatgaccgtaccgatagcaccgcccgccactgcctgcgcgctgaacgcatcggagaaaatcagcttgacggcaggcatcagtgcatcggaattaatcgcgataatggaaagaccgcccaacacataaaacaccgccatagcaggcacgatgaaagaagcggctttggcgatgcctttaataccacctaaaacgacaacggcagtcagaacggtcaacgtaatgccggtataggcaggttcgataccgaagctggtttgcaccgcctgtgcaaccgagttggactgcaccgagctgccgataccgaaggaagcgaatgtgccgaacagcgcaaacgcgacggccatccatttccagtttttgcccaagcctttttcgatgtaatacatcgggccgccggacatttcgcctttggaattgttgacgcggtatttcaccgccaacacgccttcgccgtatttggtggccatgccgaaaatggcggtcatccacatccaaaataccgcgcccgggccgccggttaccaccgcagtcgccacgccggcgatgttacccgtgccgatggtggcggacagcgcggtcatcaacgccgcaaaatgggaaatatcgccttcgtggccttcgccgcttttatgcttctttggcggcataaacgcctgtttcagcgcataacccaacatcgtgaactgcaaaccttttaataaaacagtcagcaaaatacccgtgccgaccagcagcatcagcatcaaaggtccccaaacccagccgctgacggtttcaaaaaaggctttgggattgtctaaaaacacttgcatggctttctcctttgtctgttttatttttaaaacaccacttttgtagtgtccagtaatttcagcacagaatatccaataagacaatatgttcttttgaaaaatacttttggttttttcgccgaaaacaggacggttcaagttgcggaaattgtttgcaattctttaaaagcagcggcggaggtcacaatgaaatgtccgaatggggatgtggcgggcggcagaaatcatcaatgctgccgactgccatacttctgaaatctacaaaatgatgcatcgatcaaacaatataccgctttaaaaaaaccgatgccgtctgaaacgctttcggggtttcagacggcatcaaaagggtacggtcagcggatgatgccgcgcgccgattgtgcgaaaaagtctcggaatacggcaagctcggcttgggtttcggcgcggcggagaatgtctgccttggcttcttcaaacggaatgccgcgatggtagagggttttgtacacgtctttgacggcggaaatctgctctgcggtaaaaccgttgcggcgcatgccttcgctgttgagccccgccggttcggcgcggtagcccgatgccataaagtagggcggcacgtctttgtgtacgcctgcggcaaacgcggtcatggcgtagtcgccgatgcggcagaattggaaaaccagcgtgtagccgcccaaaacgacgtagtcgccgatggtaacgtgtccggcaagcgaggcgttgttggcgaaaatggtgtggttgccgatgacgcagtcgtgcgcgaggtggcagtacgccataatccagttgtcgtcgccgatacgggtttcgccgatgccggttaccgtacctaaattaaaggtggtgaattcgcggatggtgttgccgttgccgataatcagcttggtcggctcgtcgcggtattttttgtcctgcgggatttcgccgaggctggcaaattggaaaatgcggttgttttcgccgatgctggtgtggccgttgatgacggcgtgcggaccgatttcggtattcgcgccgatttggacgttggggccgataacggtgtacgcgccgactttgacgccggagtcgagttcggctttggggtcgatgacggcggtcgggtggatgagggtcatgtttttcctttcctgtcgtgttgccgcgaagatgcgcgacggcaacaggttgtctgaaaactttcagacgacctttttctgaacactcaaaccacgcgtttggcacacatgatgatggcttcgacggcaacttgcccgtccactttggcaacggcgttgaatttgccgatgccgcgccggctggtcagcagctcgacttcaaagacgagttggtcgccggggatgacttggcgtttgaaacgggcttcgtctatgccggcgaagaagaagaattcgttttctttgcgcccgccttcgctcaaaatcgccaacgtgccgcacgcctgcgccatcgcttcgatgatgagtacgccgggcatcacgggcaggtcggggaaatggccttggaactggggttcgtttatggtgacgtttttaatcgcggtcagggttttcatcggctcgaaggcggtgatgcggtcgagctggagaaacggatagcggtgggggatgagtttttggatgtctttggcttcgatggggagttgtacgtccatgtctgtcgtattccttgaataaagtcggtttggttatttgctgtcttgaccggcatctgaaagctgctgctccagtgttttgagccgtttgttcatttcgcttaagcggtggatgtaaacagcgttgcgcgcccattctttatgggtggacatcgggaagatgccggcgaggtgtttgccgctttcggtaatgctgtgggtgacggacgtgccgccgccgatggtggttttgtcggcgatttcgatgtgtccgaccgtaccgacgccgccgccgatgatgcagtagctgcctatggttacgctacctgagatgccggttttggcggcgatgacggtgtgcgaaccgattttgcagttgtgtccgatttggacttggttgtcgattttggtgccgttgccgacggtggtgtcgctcatcgcgccgcggtcgatgttggtgttcgagccgatttctacgtcgtcgcccagcgttaccgcgccggtttgcgggattttgaaccacgaatcgtcggcgaaggcgagtccgaaaccgtccgcgccgatgaccgcgccgctgtggatttcgacgcgtctgcccagtgtgcagccgtaataaacgacggcgttgggatgcaggacgacttcgtcgcccagtttgcaatcgtgttggacgacggcgtttgccaagatgcggcagccttcgccgagcacggtgtttgcgccgatgtagacgttcgcgccgatttcgcagctggtgggaacggtcgcgcccggttcgacgacggcggtcggatggatgccgccgcgcgctttgacgacgggtgaaaacaggcgggcgactttggcgaaatagagataggggtcgtcggcgacaatcaggttgcgcccttcaaatccgtctgccgctttggcggaaacgatgaccgcgcccgcgctgctgtcgtggacttcggctttgtatttcggattggcaaggaagctgatgtgttccgcctgcgcgtctgcgagcgggcgcacggcggtaacggaaatgtcctcgccgcgccattcgccgccgagccgcgcggtgatttgggacagggtgtaggtggccggaatcatggttttcctgttcggtatgccgtctgaaagggtcagcgggcgttcatttctttaatgacgctgtcggtaacgtcgtattgggtgttgacgtaaatcacgttctgcaaaatgacatcgtaaccttcctgtttggcgattttgacgatgacgcggttggcgttttgctggagggaggcaaactcttcgttgcggcggaggttgtagtcttcttcaaactgcgcctgttttttgcggaacgctgcgaccagcccgcgccatttttcttcggcttgcgccttttttgcgtttctgagtttgccttcggcaagctgcctttccaaatccagaccttcgcgttgcagtttttgcaattcgtcctgacgagcggaaaattcgctgtccagcgttttttgaatcttgcgcgcctgcttggattcgaggtagatgcgctcggtgttgataaagccgattttttggaaggtgtcggcgtgcgcgcctgcggtgcagcacaaaccgatcagagccgcggcaaacgcgcgggtcaaacgggtcatggtaaaactccttcgaatgttgccgcgaaatgccgtctgaagggcttcagacggcatttgcgggattagaacgtcgtgccgagttggaattggaagcgttggatttcgtcttccggttttttcttcagcgggtaggcgtagctgaatttcatcgggcctaaaggcgagagccaggtaaccgcgccgccggcggaatagcgcaattcgttggtaaaggtggatttatgggtattgccggcgccgtaaatgttttgaaccctgccgccggtcgcggaactgctgttgtcgtcgtaggttttgccgtcccacacgctgcctgcgtcggcaaacaggctcaggcggacggtgcgcgcgtctttcgcgccgggcatcgggaagagcagctcggcggagacgttggcttttttgttgccgccgtagctgattttttcgccgtattcgtcatagactttcggaccgagcgtgccgctttcgtatccgcgcaccgaacccaggccgccgccgtagaagttttcaaagaaggggatttctttggttctgccgtagccgcccgcaatgccgacttcgccgccgagcatcagcgtgaaggttttgctcagggggaagaaccaggtttggttgtgggtggcggagtagtattgcagtttgctgccaggcagggcgatttcggcgttcacgcccgtcaggtagccgcgcgtcggccataacgcgctgtcggttttgttgcgcccccagccgacggtacctttgtac

附录Bagccagcctttgaagctgccgtctgtgccgtcggttttgccgtatttcttgataaagtcggcatagtgtttgggcgctttgttgtaggtgttgacggtcaggtgttctgccaccaaaccgaaattcacgcggtcgtattcggtaacaggcacgctcatgcggatgcctgcgcctgccgtggtggttttatattgtttgatgctggtcgatgctttgcgcgggtcgaaggcttttccgtaaacatcgtagcccaggctgaccccgtctgccgtgaagtacgggtcagtaaacgacagcgagccgttaagcgtggttttgctcctggaggcgcgcagtgcggccgacttgcccgtaccgaacaggttgtcttgggaaacgcctgcggacatgaccaacccggtatcttgaacccaacccgcgctcaaatccagggaaccggtggaacgttcggtcagactcatgttcaaatcgactttgtcgggcgtgccggcaagcgggacagcatcaaactggacattgtcgaagtagcccaaaagctcgacgcgctctttggaacgttgcagcttggaggtgtcgtaaggtgcggattccatttggcgtaattcacggcggacgacttcgtcgcgggttttgttgttgccggtgatgtgtatttcgttgacgtagattttccggcccggttcgatgtgcaggacgaaatcgacggttttggtttcagcgttcggcagcggctgtacgctgatttcgctgtatgcgtagcctgccgagcccatgcggttctgaatctcacccaaaacggcggtcatctgctggcgttcgtaccatttgccgggcttcatggtcagcagtttttccagttcggctttggggacttcgttggtgtcgccttcgatggagactttgccccaacggaaacgtccgccttcgtggacggtgattttgatggtctgcttggttttgtcttcgttggtttggatgtcggtatcgaggatacggaaatcgaagtagccgttattttggtagaagtcggttactttttccatatcttgggcaaatttctgctcgttgaattggttgcttcgtgtcagccatgtccaaatgccgccttcggtcagggacatttgccgcatcagtttgcggtcggaatagacttggttgccttcaaattcgatgtcggtgattttggcggatttgccctcgtcaatcgtgatgtcgatgtcgacgcggttgcgggcgagtttggttactttgggcgtgatttggatattgagtttgccgcgcccgaggtattcttctttcaggccggcgactgcctgattgagtgtcgcctgattaaagtattgcgactgcgccagcccgaacgattcgaggtttttcttaatggcgtcgttttgcagcatttttgcgccggtgatgttgagcgagccgatggtggggcgttcgataacggtcagcaggagctgcccgtccgcagtttcgacgcgtacgtcgtcaaagaaaccggtggcgtacaggcttttgatgatggcactgccgtgtgtgtcgttgtaggtgtcgccgactttgacgggcaggtagttgaatacggtactcggctcggtacgctgcaagccttcgacgcggatgtcttggatggtgaagtcggcaagtgccaaaggcgatatgcccaacatcatcagtgcggaagcaatctgtttcagtttcattgtcagttccttgtggtgcggaatgcggtttcagacggcattccgaaacgtaaaatctaaccgagcagccgggtaacgtcgttgaagaaggcgaccgccatcatcagcatcatgagggcgagcccgaagcgcaaaccgatgttttggacgcgttcgcccaaaggtttgccgcgtatccattcggcagtataaaacacgaggtgcccgccgtccaaaacagggacgggcagtaggttcagcacgccgaggctgatgctgaccagtgctaaaaattccaaataactttgcaagccgagttcggcggactgtccggcaatgtcggcaatggtcagcggcccggaaatatggctgacggaggcgttgccgctgattagtttgccgaaaaatttgagggttgtccacgagtgggaaacggttttttcccagcccatgccgaatgcgcggacaacagacggacggtagctgcggcggatttgcgcgtcccacgccctgtccggctgcggacggaggccgacgcgcccgatcagggtgtggtcggactgttcgacagtatcggggcggatgtcggcggtatgggtttgtccggcgcgttcgtagttcagggtgatttttttgccggggctttggcgggtcaggtttgcccattcttgccatgaggcgatgggtttgccgtcggcggcagtcagcctgtcgcccggtttcaggcctgctttttcggcggggctgcctttttccacgccgccggcaacggttgtgattttaaagggcatcagtccgatgtagccttggttttttgcgattttaccggcttccggcgtgcctgcggcatcgatggtgcggacggtttgcgcgcccgatgccgtctgaacgccgacggcgactttgccggcttcgaggttgaggacgatttcggtttgcgcgctgccccaatctgcaacgggtgtgccgttgacggattgtattttgtcgccgctttggaagccggcgcgggcggcaatggtgtcgggttcgactgtgccgacgtaggggcgcagttcggttacgccgaaggaaaagctcagtccgtacagcaaaaccgccagtgcgaggttggtcagtgggccggcggcgacgatggcgatgcgcttggcggggtgttgtttgtcaaaagcgtagggtaaatcggcttctgatacttcgccttcgcgcgtatcgaccattttgacgtaaccgcccaacggaatcggggcgaggcaccattcggtgtcgccgcgctttcgggtgaaaaacggtttgccgaagccgacggaaaagcgtacgactttgacgccgcacaatctggcaacgatgtagtgtccgaactcgtgcaggctgaccaaaatcaggatggcgaagataaaagctagaagggtgtgcaaatggttttcctttgataacggtgttcagatggcatcagcgcagtgtgccgataaatgctcgcgcttgtgcgcgtgtccgggcatcttgcgccaagagcccccctatatcgcctatgccgtctgaaaagtcttgtgcaagacagtgggcgacggttttggcaatgtcggtaaacttaatctgtccgtccaaaaaggcggcgacggcggcttcgttggcggcgttcaatacgcagggcgcggctccgcctgcgttcatggcttcataggcgagcctcaggcaggggaagcggtcaaagtcgggcttttggaaggtcagcgcggacaatgcgtcgaaatccaggtcgccgacacccgaatcgatgcgctcgggcaaacccaaacaataagcgatgggcgttcgcatatcgggattgcccagttgcgccagcacggagccgtcgcggtagcgcaccatgctgtgtatcacggattgcggatggatgacgacttcgagtttgtcgggcggacagttgaacagccaatgcgcttcaatcagctccaaacctttgttcatcatggtggcggaatcgacggagattttgcgtcccatacgccaattggggtgtttgaccgcttgggcgggcgtaatgcggtcgaacgtgtttaaatcggcggtcagaaacgggccgccggaagcggtcaggataatcgaagcgatgccgtgttcgttcagacggccggcgtaatcgcgcggcaaaacttggaaaacggcgttgtgttcgctgtcgacgggcagcactgccgcgccgtttgcacgggcggtttccataaacaacgcgccggaaaccaccagcgtttctttgtttgccagataaatggttttgcctttttgcgccgctgcgagcgcggaaggcagccccaccgccccgacgatggcgcacatgacaccgctgacttcgtcggcagaggcaacgtcaaccaatgcctgcgcgccgtgtaaaacctgagtcgccgtgccgtcgcgtttcaacagggcttcaagccgggcggcgtgttccgcatcggcaacgacggcatattcggggtggaacgtttgacattgagccgccaatttctcgacctgcttatgccctgccagcgcgaatacgcggaatttttcggggtggcgggagacaacgtccagcgtgctttcgcctatgctgccggtactgcctaatatggtcaggacttgtggtgtcataatggggataactttataccggatgccgtctgaagcgttttcagacggcatagaatcaatttaaaaccgacatcatcgctgcatagacgctgataacggcaatcaggctgtcggtacggtcgaacacgccgccgtgtccgggcagcagcttgctgctgtctttgatgcctgccgcgcgcttgagccagctttccaaaaggtcgccgcatacgctgacaacggtcagcaccaaaccgattaacacggtatcgaaccagcctgtatcgaatgccagccagccggcacttcgtacggcggtcatgtacactgccacgcaaaccgcgccgccgattgcaccttcccagcttttgccggggctgattgccggcgcgattttgtgtttgccgaacgccttgccgctgaaatacgcgcaaatatcggcaacccacaccaaacccatcacggcgagcagcggcagggcatcatcgggatgcgggcgcagggatacgagcgcgaaccaaaacggcatgaccagaagccagccgacggcataaacctgccaaccgccgttgagcctccatttgaatctcaaccataaaggcataacggcgagccaaaatgccaaaacaacataccaaaccaaattaggcagcatccagccgcccgcataggcaaccacgccgaaaaccaaggttgcggcgaggtaatggttggttttaattttgcacaaaccgcccatacgggcatattcccacaaggcaatcagggcaatcagtccgcaaaatgcagcccacaaccattgcggcgcgtaaaacagcatgcccagcatcagcggcagcagccacatggcggttattacccgttgtttcagcatattcagttcctttgctgttcgataggcagttgctcggaggtgcgtccgaaccgccgttcgcgtttttggaacgaagcgacggcatcgtccaaagccttgccgtcaaaatcgggccacaaaatatcggtgaaatacagttctgcatatgccatctgccagagcaggaaattgctgatgcgcgtttcgccgccggtgcggatgaacaaatccggttccggtgcatcgcccagcatcaagtgtttcgccagcgtgtcttccgtaatctcggatacgccttcggcaatcagtttgtttgccgcctgcaaaatatcccagcggccgccgtaatcggcggcaatgctcagggtcaggccggtattgtttgccgtcaacgcttccgcctcttcgatgccttgcagaatctgccggttgaagcgttcgcggctgcccaatatcttcaggcgcatattgttttcgtgcaggcggcgtacctgtttttgcaaagcctgtaaaaacagccccatcaggaacgaaacttcgtcttcggggcggcgccagttttcggttgaaaaggcaaacacggtcagatattgcacacccagtttggcgcaatgcttcaccatattttccaatgcgtccaaaccgcgtttgtgtcccattatgcgcgggaggaaacgttttttcgcccaacggccgttgccgtccataatcacggcgatatgcttgggaatggcggtgtgttccaaaacggcctgcgtgctgcttttcatgtctgcctttcgcggttcggcattcaaatgccgtctgaacgccgaaccgtgcaggttaaattgccatcaaatcttcttctttggcagtcaggagtttgtcggcttcggtaatgtatttgtcggtcagtttttgaaccgcttcttcgccgcgacgtgcctcgtcttcggaaatttctttgtctttgaggagttttttgatgtggtcgttggcatcgcggcgcacgttgcggatagagacgcggccttcttccgcttcgccgcgtacgactttaatcaggtctttgcggcgttcctcggtcagcatgggcatcggcacgcggatcaggtcgccgacagctgccgggttcagtcccaagtttgaatcgcggatggctttctcgactttggccgccatattgccctcaaacggtttcacgccgatggtgcgcgcgtccagaagcgttacgttggcaacttggctgacggggaccatgctgccccagtattcgacttccacttggtcgagcaggccggtatgcgcgcggccggtacgcactttcgccagattttctttcagtacttcgaccgaacgctgcatcttgccttcggctgttttttgaatatcgttgatcatattgttctttcggtgggataaggtgggcgggagaccgtctgaacgcgtttcaagccgttcagacggcataaagaccgttaaccgcgaatagtaccgttattcgggcataacgacaaggtaggc

附录Bggattggggatgccgtctgaagcgacaggcgtttcagacggcatcgtgtccgaccgtcagccgtgttcccgtgtttcaagcaggctttggcgcaggtgttggcgttcgtgggcatccagccatttgcggcgggtgcgttgcagcaggatgacgagggcggaaatttcctgacgcatattggtgctgagccagaggaagccctgccattggtagtggaggtgttcggcgagggcttccagttcggggttgatggcggtgtcgatgcggatgcggcgggcgtgtctgccgttgataagggcgacggtttgttgcaggtcggtttggagcagtgtgaagtggcggtcaagcagccggatttcgctgccgttgagtttgggagattgcagcttggcggcggtggtcaggagcagctcggtggtgttgacgattttacggtgggcgtgctgcatggcttccatcatggcggggctgatgcggctttcgcccgatgtggcggcgagatggctgcggcttttgaccatgcgtgcgttgatttggcgcattttcgccatgttctcctcgaggcgttcgcgggtcatgcgcctgccgttgctgatttcggcaatcattttgctgcagtcggccaggttgtcggcaagcatgaaacgccacatcagtgtggatttcagcggcagcagtttggcggcggcgatggcgatggccgcgccgatgaggacgttcatggcgcgcatgagtccgctgtcgagccattcgctgccgttgtcgccgatgagcatacacatcgtcagccctgccagcatagggacgtagccgtttttgccgaccgccgcccagccggccagtgcgcttgccgtgccgacggtgaggtagaagaggaggttgccgtggaaataatgctggttcagccataaaacgcccaaacccgcgcccagcccgatgaccgtgccgagcatacgttccaccgccttggagtaaatcgccccttgaaactggagcatgccgaggacgacgaagacggtcatccctatccactcgccgtgttggaggtggagcagccgggcggaggcggtggcgaacaggacggccccgccgagccggacggcgtggatgaggcggcggtagcggtagcgttcgtaggagttgagccagcggctgacgaggcggttgcgttgcgaggtgttcatatcggttgtgccgtctgaagcggaaatgtgaaaaagcacaggcttcccgaggaagggagggtctgtgcttggtattggtgccggagaagggaatcgaacccccgaccttcgcgttacgaatgcgctgctctaccgactgagctacaccggcgttttttcgtcatgatatatatgaacggttgtttgtgcaacttttcgggcgggcggcaaggcagtgcgcggtatagtggattaacaaaaaccagtacggcgttgcctcgccttagctcaaagagaacgattctctaaggtgctcaagcaccaagtgaatcggttccgtactatttgtactgtctgcggcttcgtcgccttgtcctgatttttgttaatccgctatataatgcggtctgcttcggaagagggggacggcgatgtttgtgaacgagaaatatccttatgcggctctgtttgcgggactggtgtttttgacgctgccgtttgcgttggcggtgcatgatgcctttgcgcttgcgttcggacggacggggttgctggtgtcggtgtcggacggcggattcggctggcgtggcggttgggacggcactgtttggtttgtgttcggtgtgtttgcgtttttgaatgtggttgtgtcggcgggtctgacgaaactggcgtacaaaaagatgatgcggcggcattcgcgttacacactgtttctgtcgggcgtggcggcttgcgcggcggcagcggtggcttggattttcgagctgctgcttggcagtggggctttgggcgggctgcgggggaggcggtgttggaatatgcgtttgccgtgtggctggtggcgatgctgacgctgcccaaacgcctgacgcgcgcgccggtgcagccggtggtgtttcacaggaaaaaataggttggaacgggaaatgccgtctgaaacccgacacgcggtttcagacggcatgtttttccgctaacattacgcctgaatatggacaggaagcagatatggaacgcaaagaacgcctgcgtgcaggcattgccgcgatggggctggatatttcggaaacggcgcaggacaggcttttggtctatgtggatttgttgaaaaagtggaacaaaacctacaatctgaccgccctgcgcgacgaggaaaaaatgattgtccatcatcttttggacagcctgacgctgctgccccatatcgagggtgtgcaaacgatgctggatgtcggttcgggcggcggtcagcccggcattccggcggcggtgtgccgtccggatgtgcaaataacccttttggatgcgaatacgaagaaaacggcttttttacagcaggcggttatcgagttggggttggacaatgtgcgcgtggtatccggacgcgtggaggcggtttcggacgtgcgtgccgatgtggttaccagccgtgcgtttgcagaactggcggattttgtgtcgtggacggtgcatctgttgaaagacggcggctactgggcggcgatgaagggcgtgtatccgcaggaagaaatcggccgcctgccgcaggatgtgtgcgttgaaaaagtccaaaggctcgacgtgccgggcttggatgcggaacgccatatcgtcatcctgagcaagcgttgagcgcacttcagacggcatgaataccttttttgtgcggataaaggtaaaattccgcactgtttttcttttttcaacatcagacgggacacgggcgggacatgagtgcgaacatccttgccatcgccaatcagaagggcggtgtgggcaaaacgacgacgacggtaaatttggcggcttcgctggcatcgcgcggcaaacgcgtgctggtggtcgatttggatccgcagggcaatgcgacgacgggcagcggcatcgacaaggcgggtttgcagtccggcgtttatcaggtcttattgggcgatgcggacgtgcagtcggcggcggtacgcagcaaagagggcggatacgctgtgttgggtgcgaaccgcgcgctggccggcgcggaaatcgaactggtgcaggaaatcgcccgggaagtgcgtttgaaaaacgcgctcaaggcagtggaagaagattacgactttatcctgatcgactgcccgccttcgctgacgctgttgacgcttaacgggctggtggcggcgggcggcgtgattgtgccgatgttgtgcgaatattacgcgctggaagggatttccgatttgattgcgaccgtgcgcaaaatccgtcaggcggtcaatcccgatttggacatcacgggcatcgtgcgcacgatgtacgacagccgcagcaggctggttgccgaagtcagcgaacagttgcgcagccatttcggggatttgctttttgaaaccgtcatcccgcgcaatatccgccttgcggaagcgccgagccacggtatgccggtgatggcttacgacgcgcaggcaaagggtaccaaggcgtatcttgccttggcggacgagctggcggcgagggtgtcggggaaataggtcaatccaaatcgggctgcccgtgcctttatgctgtttggccgggtgcgttatagtggattaacaaaaatcaggacaaggcgacgaagccgcagacagtgcaaatagtacggaaccgattcacttggtgcttcagcaccttagagaatcgttctctttgagctaaggcgaggcaacgccgtactggtttttgttaatccactataatatggcggattaaaataaaaatacttatatcgtcatttatcgtcattcccgcaaaaacaaaaaaatcaaaaacacaaaactgaaatatcgtcattcccgcgcaggcgggaatctaggtctgtcggtacggaaacttatcgggaaaaacggtttttccaaccctgagactccggattcctgttttcgcgggaatccggttttttgagtttcagtcatttttgataaattcttgcagctttgagtttctagattcccgcttttgcgggaatgacgcggaaaagttgctgtgatttcggataaattttcgtcacgcttaatttctgttttatccgataaatgcctgcaatctaaaatttcgtcattcccgcaaaaacaaaaaatcaaaacagaagcctaaaatttcgtcattcccgcgaaggcgggaatctaggtctgtcggtacggaaacttatcgggaaaaacggtttttccaaacctgagactccggattcctgttttcgcgggaatccggttttttgagtttcagtcatttttgataaattcttgcagctttgagtttctagattcccgcttttgcgggaatgacgcggaaaagttgctgtgatttcggataaattttcgtcacgcttaatttctgttttatccgataaatgcctgcaatctaaaatttcgtcattcccgcgaaggcgggaatctaggtctgtcggtacggaaacttatcgggtaaaacggttttgccagccctgagactccggattcctgttttcgtaggaatccggttttttgagcttcagtcatttttgataaattcttgcagctttgagtttctagattcccgctttcgcgggaatgacggtttggaagttacctgaaattcaaaaaaaaaacggaaaccggacggattggattcccgcctgcgcgggaatgacggattttaggttttttttttgattttctatttttcgcgggaatgacggtttgggttctttctctttggagttgcgatgccggaaatgccgtctgaaggcttcagacggcatttttgtgccggtttaaaacaaggcctgctgcgcgagcaggtttctgacgggggcgaagtcgcggcggtgttcgggcagcacgccgtatttttcgagggcttccaaatgctgcttcgtgccgtaacctttgtgtttgtcgaaaccgtattggggatggcgttgcgccagtgcgtacatttccgcatcgcgtgcggtctttgccaaaacggatgcggcggagatttcgatgattttgctgtcgcctttgacgacggcttcggcagggatgttcaaatgttcaggaatgcggttgccgtcgatgaatattttttcgggacgcacagccaagccgtcaacggcgcgtttcatcgcgagcatggtggcgtgcaggatgttgaggctggcgatttcttcgggcgaggcggcggcaacgtgccactcaaccgcctgattttttatcatttcggcaagcgcgtcgcgttttttctcgctgagttttttggagtcggtcagtccgggcaggtcgaatgtttccggaaggatgacggcggcggcaaacacgctgccgactaaaggtccgcgtcctgcctcgtccacgccggcggtcagtatgtgcatgatgtttcctgtcgggatggtgggaatgccgtctgaaaagggtttcagacggcatcgcgccgatgtgtttatttcgcgtctttaaacccgcgcttcaaatgcaccatcagcaatgccactgccgcaggggttacgccggaaatgcggctggcttgtccgacggtttcgggtttgtgctggttgagcttttgctgcacttctgccgacaagcctttgactttgccgtaatcgatgccgtcgggcagttttaaggtttcgatgtcgcggcggctgtcgatttcttcgttttggcggtcgatatagccttggtatttgacttggatttcgacttgttcgatgacttcggcggagaggttttcagacggcatcgcgccttcgagcgtcatcagcgcggcgtagtcgaggtttgggcggcgcaggaggtcgtgcaggttggcttcgcggctgagtttttgtccgaacacacggatttgttcgccttcggcgagtttttgcggcgtgtaccacgttgttttcaaacgttggatttcgcgttcgacggcttcgcgtttttcgttgaacatgcgccattgcgcttcggacaccaagccgattttgtagccgtcttcggtcaggcgcatgtcggcgttgtcttccctgagttgcaggcggtattcggcgcggctggtgaacattcggtagggttcgttcacgcctttggtgatgaggtcgtccaccaatacgccgaggtaggcttgttcgcggcgcagcaggagcgggtcttgtccgcgcacatattgcacggcgttcgcgcctgccaataaaccttgcgcggcggcttcttcgtagccggtcgtaccgttgatttgcccggcgaaaaacaatccggcaatggttttggtttcgaggcttgctttgaggttgcgcggatcgaagtagtcgtattcgatggcgtagccggggcgcaggatatgggcgttttccaaacctttcatactgcggacgagcgcgatttggatgtcgaacggcaggctggtggagataccgttaggatagtattcgtgcgtggtcagaccttcgggttcgaggaaaatctggtggctgtctttgtcggcgaagcggttgattttgtcttcgatagacggacaataacgcggacccacgcct

附录Btcgattttgccggtaaacatcgggctgcggtcgaagcctgagcggatgatgtcgtgggtttgcgtgttggtatgcgtaatccagcaggacacttggcgcgggtgcatatcggcgttgccgcgcacggacatgacgggaacgggcgtgtcgccgggctgttcggtcagttgggagaagtcaatcgtgcgtccgtcaatacgcggcggcgtgccggttttcagacggccttgcggcagcttcaattcgcgcaaacgtccgcccaacgatttggcggcggggtcgccggcgcgtccgccttcgtagttttccaaaccgatgtggattttgccggacaaaaacgtgcctgcggtcaacacgacggcgcgtgctttaaactccacgcccatcgcggtaattacgccgctgatgcgttcgccgtcgagcgttacgtcttcgacggcttgttggaaaaggtcgaggttttcttggttttccaacatttcgcggatggcggctttgtacaggatgcggtccgcctgcgcgcgcgtggcacgcactgccgcgcctttgctggcgttcaggcggcggaactggataccggatttgtcggttgccaacgccatcgcgccgccgagcgcgtcgagttcgcgcaccaaatgccctttgccgatgccgccgatagaggggttgcacgacatttgtccgagcgtttcgatattgtgtgagagcaaaagcgtctgcgcgcccatacgggcggcggcgagtgcggcttccgtgccggcgtgtccgccgccgacgacgataacgtcgtaggttttggggtaaatcatgtgggtcatagtgtgtattgcctgacggtgtttcagacggcatttatagtggattaacaaaaaccagtacagcgttgcctcgccttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggtttcgtactgcttgtactgtctgcggcttcgtcgccttgtcctgatttttgttaaaccactatattcaatatgccgtctgaaaaacgaaatggattcaaaagtaaagggttgggattgtacgcttgttcgccctgtttttacagtgtgcggaaagggaaaagccgcttcgcggggaagcggctccggtaagggcgggatttaccaaacgtcggatttgatacggcgtttcaggcccggatgttcggaaagtttgaactcggggtctttgcccattttcagcttggcggtgtaatcgcgcagcagcataaacgccaagggcgagagcagcaggatggcgacaaggttgatccacgccataatgcccatcgccatatccgccatatcccagaccaaaggcacattggcaaccgcgccgaaatagacccacgccaaaaccagcatacggaaaacggcggtaatcagccaatggcttttgatgaattggacgttggactcggcataggcatagttgccgataacggtggaaaaggcaaacataaacaggatgacggcgaggaagcccgcgccccattgccccacttggctgacaatcgccgcctgcgtcagcgccgcaccgctcaaatcgccgtaaggctgttggtaaatcaagatgatgaaggcggtgcaagaacaaacgatgatggtatcgacaaacacgcccagcatttgaatcataccttgcgaaacagggtgtttcacttcggcggcggcggcggcgttcggcgcggaacccatacccgcctcgttggaatacaggccgcgtttgatgcccatcatcatcgtttgcgaaatcagaccgccgagtaagccgcctgctgccgcgtcgaatttgaacgcgcccgaaaaaatctgaccgaacacgtccggaatcatcggaatattggtcaaaatgatgaaaagcgcgataaagaggtacaaaaccgccatcagggggacgacgatttccgccgctttagatatgcgcctgatgccgccgaagataatcggcgcggttaaaatcaccagggcgacgccgacataatgaggctcccaaccccatgccgctttgacggtatcggcgatggtattggtctgaaccgcttcaaacacaaagccgaaacagaaaatcaggctcagggcgaacaacacgcccagccatttctgccccagcccttgagtgatgtagtaggcagggccgccccggaaatggtggttgtcgtagtcgcggactttaaagagctgcgccagcgaagattcgacaaacgccgaactcataccgattaaggcggttacccacatccaaaacaccgcgcccggtccgccgactttgatggcgatggccacgcccgcgatattgcccacgcccacgcggctggcaaggccggttacaaatgcctgaaacggcgtgatgccgtgagggtcgtccccctgtttgcggccgccgagcatttctttgatgctgcgcccgaacaggcggaattggacaaagcccgtggttacggtgaagaaaagccccgtacccaaaagcatataaaccaagtatgaccacatcggatcgttgatggcgccgacccagccgtgcagccattcggtaaagttctcgttcatatcgcttccttaaagttgaaactcgcacatattggcggtatgcaagcagggtttaaattttgtaaacgcccattctagcagattgtcaacaaaatcagaaaaatttacatcgccgcgcggctgcggcgttagaatcgcattttgtttggagcaaacacgatgaaacagcctgtttttgccgttacttccggcgagcctgccggcatcggccccgatatttgtttggacttggcgtttgcacgcctgccctgccgctgcgcggtattgggcgacaaaaacctattgcgcgcgcgcgccgaagccttgggcaaaagcgtcgtcctgcgcgacttcgatccagaatcaggcggcgcggcatacggcgagctggaagtgctgcacatccctgccgtcgaagcggttgaggcgggcaaactcaatcccgccaacgccgcctatgtgctgcaacttttggacaccgcgctcgcaggcatttcagacggcattttcgacggcatcgttaccgcgccgctgcacaaaggcatcatcaacgacgcgcgcgcaagcacaggttttttcagcggacacaccgaatatctggcggaaaaaagcggcacggggcaggtcgtgatgatgcttgccggcaaaggcctgcgcgtcgccctcgtaacgacccacctgccgctgaaagacgttgccgccgccatcacgcaaccgctgattgaaagcgtcgcacgcattttgcatcacgacttaaaacacaaattcggcatcaaaaatcccaaaatccttgtcgccggacttaatccccacgccggcgaaggcggacacctcggacacgaagaaaccgacaccattatccctgcattggaaaacctgcgccgcgaagggataaaccttgccggcccgtatccggcggacacattgttccagccgtttatgctcgaaggtgcggatgccgtattggcgatgtaccacgaccaagggctgcccgtgttgaaataccacagcttcggacagggcgtgaacatcacgctcggcctgccctttatccgcacctccgtcgatcacggcaccgcgcttgatttggcggcaaccggcagggcggattccggcagcctgataactgccgtggagaccgccgtcgagatggcgcgcggcagcctttaaagatgataaaagacccgtcatttccgcgcaggcgggaatccggtctgttcggtttcagttgtttttgggtttcgggtaatttccaaatcgtcattcccgcgcaggcgggaatccagaccattggacagcggcaatattcaaagattatccgaaagtttgaggttctagattcccgttttcacgggaatgacgaaaggtggcgggaatccggtctgttcggtttcggtttttttttttgaggtttcgggcaacttctaaaccgtcattcccgcgcaggcgggaatccagaccattggacagcggcaatattcaaagattatctgaaagtttgaggttctagattcccgttttcacgggaatgacggaatgttgcgggaatccggcttgttcggtttcggtttttttgaggtttcgggcaacttctaaaccgtcattcccgcgcaggcgggaatccagaccattggacagcggcaatattcaaagattatctgaaagtttagaggttctagattcccgttttcacgggaatgacggaatgttgcgggaatccggcttgttcggtttcggttttttttgaggtttcgggcaacttctaaaccgtcattcccgcgcaggcgggaatccaggcctttgggcgacggcaatattcaaagattatctgaaagtttagaggttctagattcccgttttcacggaaatgacgaaatgttgtgggaatccagaccttcgggcagcggcaatattcaaaggttatctgaaagtttgaggttctagattcccgttttcacgggaatgacgaaaggttgtgggaatccagaccttcgggcagcggcaatattcaaagattatccgaaagtttgaggttctagattcccgttttcacgggaatgacgaaaggtggcgggaatgacgaaaggttgcggtaatcatgggaatggcgaagtttcagacggcatcgtccaccctccgccgtcattcccgcgcaggcgggaatccaggcctttgggcgacggcaatattcaaagattatccgaaagtttgaggttctagattcccgttttcacgggaatgacggaatgttgcgggaatcatgggaatgacggaatgttgcgggaatcatgggaatgacggaatgttgcgggaatcatgggaatgacggaatgttgcgggaatcatgggaatggcggaatgtttcggtaatcacgggaatggcgaagtttcagacggcattgcaggtatccgaacccatgtaaaaaagaggttctgcggaacagaacctctttttgccgccgtcggttcagccttgccgggtttcgacttggatcatttcttcggcagggacggttgcgacttcagacggcttgggctgttcggaacggcgcaaaccgcgtccggcttggacttcgggttgtgccgcccatgccttcaatgcggcagggtcggtttcgatcaggacgagtccgccggtttgtgcggtttcccgtgcctgttccgccgcagccgtaaaggttgcggtttcagacggcatttcctgtgcttcggctttcggtgtcgcgccttcgggcaggatggcggcggtggcacggcggattttttccgccgcatcataaaccggtgcgtcgccgtttgaaacggcgggagatgctgtcggaagatccctttctgcaaccggatcggcaatgctgacagtaatcggcgcgtttgcgtcggtttcgccgaaaacgtgcgcggcggcggaacggactttgtcggcggtgtcgtgaatattcaggtactgctcgattttggcggcagacggaatattgcgttttttgccgttttgacggcggtcgcgctgattgttgcgctcgcggcgttctttggcatctcggctgtcgcgttcgcggcggttgcgttcggatttgggcttgctgcctttgtcttctgcggtatgcggttcggacggcgtgttttccgctgtctgaacggttgtttcggcaacggtggcggtttccggcgcggtttggccgcgttcgctgcggctgccgttgcggcggcgttttccggtttgcacttcggtttcggacggtgcggcatctgcaacggttgcggcaggctgtacgttgcggctttggatttccgcttcgttggcgcgttcggcggcacggtcgccgcgttcattgcggcggcggttgccgttgttgcgcgtttcggctttgtcggcacgcgcttcctgtccggcagttttgcctgccacttcgcggacttctactttgctgccttcgcgtttgctgcggcgcgggttttggcggcggttgttggcgcggctgccgctgcggtttgccgtgctgcgtttttcggaggtttcggcagcgggcgcggcttgggtttcgctgccgccgaaaatgcgtttgagccatgctttgaagctgtcccaccaagaggtttttttctcgggggcggcagtcggggcggggctggtgtggcgcacgcctttgacggcgggttcgggacgggcggctttggctttttcgccgccgaacggtttggcggattcgtcttcttccggctcggcgacgcgtttgtagctcggttcgccgtcttcttctacgtcgtcggtgcggatgcggttgatttcgtagtgcggattttcgaggtggatgttcggaatcaggacgacgttgacatccaaacgctcttccatcgcaaacagctcggcgcgtttttcgttcagcaggaaggtggcgacatcgacgggcacttgtgcgcgcacttctccggtgttgtccttcatcgcttcttcttgaatgatgcgtaaaacgtgcagggcggtggattcgatgccccgaatcacgccggtgccggcgcagcgcggacaggcgacgtggctgctttcgcccaaagccggtttcaaacgttggcggctcaattctaaaagtccgaaacgggagagtttgccca

附录Btctgcacgcgggcgcggtcttttttgagcgcgtcgcgcaggacgttttccacatcgcgctggtgtttggggttttccatgtcgatgaagtcgatgacgaccaagccgcccaagtcgcgcaggcgcatttgtcgggcgacttcttcggcggcttccatattggttttgaacgcggtgtcttcaatgtctgcgccgcgagtggcgcgtgcggagttcacgtcgatggagacgagggcttcggtatggtcgatgacgatcgcgccgccggagggcaggctgacgctgcgcgaaaacgcgctttcgatttggtgttcgatttggaagcgggaaaacagcggcgtgtggtcttcgtagagtttcagacggcctatattgcccggcatgacgtagctcatgaactcggcaacttggtcgtaaacttcttgattgtccaccaaaatctcgccgatgtcggggcggaaatagtcgcggatggctcggatcagcagcgagctttccataaagagcaggtaggggtcgtgatgcgcttttcctgcttcttcaatcgcctgccagagttgtttgaggtagttcaagtcccattccaactcttccgcgctgcggccgatgccggcggtacgggcgatgatgctcatgccgttcggaatgtcgagttccgccatggcggctttcaactcttgacgctcttcaccttcgatacggcgggatacgccgccgccgcgcgggttgttcggcatcaataccagatagcgtccggcgaggctgatgaaggtggtcagcgcggcgcctttgttgccgcgctcgtctttttcgacttggacgatgacttccatgccttctttgagcacgtcttggatgcgcgcgcgtccgccttcgtagtcttggaagtatgagcgggagacttctttaaacggcaagaagccgtggcggtcggttccgtaatccacgaaacacgcttccagcgacggctcgatgcgggtaatgatgcctttgtagatattgcctttgcgctgttctttgcccagcgtttcgatgtccaaatccagcaggttttgtccgtcgacgatggcaacgcgcagctcttcggcctgcgttgcgttaaataacattcttttcatgatcacctcgtgggcaggcggcgttcagacggcacatgcccggttcggcattccgtaaggctgggttttccgatgttttcggataaaaccggtaatcagtttttgagttgaaaatccgcagggatgcacgttccggagaaccgtgtgcggaagggtcggatacagaaggctataaagatcgatgcggcggtttgtctgccgcgttccgaacgctgcggtcggaaaaatgggggccggcttcttcttgttatcgtgatgcctgtgttttgggcggtttgcgtttgggacttgggcccggctgccgtcttacttccgcgccgaaacggcaaaatcaattcaaacttgattacgttctgcgcctgccggctgggaacaggcgcagggaaaatgctttgcggagtgcgtttttaatataaaattccgttttaaagtaaaccgtttcaggaggcgcggcgggcgcgctttttgctgaaacggatgttcggattatagatgaaaacgcacgaaataagcaaagattcggtcagcttgataggggttgccgaacatgaggcgggtcaacgccttgataactatctgataaaaatcctcaagggtgttcccaagagccatatccaccgcattatccgcgccggcgaggtgcggttgaacaagaaacgctgcaaacccgacagccgtattgcggagggggatacggtgcggattccgcctgtgcgcgtggcggagaaggaaatgccgtctgaaaggcgtgccgccgtaccggcgcgtgcgtttgacgttgtttacgaagacgatgcgcttttggtcatcgacaaaccgtccggcgttgccgtccacggcggcagcggcgtgagtttcggcgttatcgaacagttgcgccgcgcccgtccggaggcgaagtatttggagttggttcatcgtttggacaaggatacgagcggcttgttgatggtggcgaagaaacgcagcgcgctcgtcaaacttcacgaagccatccgtaacgaccaccccaaaaaaatctaccttgcgctgggggtgggcaaactgccggacgacaatttccatgtcaaactgcccctgttcaaatataccggcgcacaaggcgaaaagatggtgcgcgtcagtgcggacgggcagtcggcgcatacggtgttccgtgtgttaagccgtttttcagacggcattttgcacggtgtcgggctgtcgcacctgactttggtgcgggcgacgttgaaaacggggcgcacgcaccaaatccgcgtccacctgcaatctcaaggctgtccgattgcgggcgacgaacgctacggcgattatcaggcgaaccgtcgtttgcagaagttgggtttgaagcggatgtttttgcacgcgtccgagctgcacttgaaccatccgctcacgggcgagccgctggtgttgaaggcggagctgccgccggacttggcgcagtttgcggtgatgttggaaaacgggacgaaaatgtgaaccccgatgccgtctgaagccttcagacggcatcgggacgtgaaagtatgtggggacagacgaatatggctgataaaaaaagccctttgattgccgtcagtgtcggcgaagcgtcgggcgacctattgggggcgcacctgatacgcgccatccgcaagcgttgtccgcaggcgcggtttaccggtatcggcggcgaactgatgaaggcggaaggtttcgagagcctttatgatcaggagcggctggcggtgcgcggctttgtcgaagtggtcaggcggctgccggaaattttacggatacgcagggggctggtacgggatttgctgtcgttgaaacctgatgtctttgtcggtatcgatgcgcccgattttaatttgggtgtggcggaaaagctgaaacggtcggggattccgaccgtgcattatgtcagcccgtcggtgtgggcgtggcggcgggaacgtgtgggcaaaatcgtgcatcaggtcaaccgcgtgttgtgcctgttcccgatggagccgcagctttatctcgatgcgggcggacgtgcggagtttgtcggtcatccgatggcgcagcttatgcccttggaagacgaccgtgaaacggcgcggcaaactttgggcgtggatgccggcatccccgtattcgccctgctgcccggcagccgcgtcagcgaaatcgactatatggcgccggtgttttttcagacggcattattgttgttggaacgctatcccgccgcacgcttcctgctgcctgccgcaacggaggcgacgaagcggcgtttggcggaagttttgcagcggccggagtttgccggattgccgctgacggtaatcgacagacagtctgaaacagtgtgcagggcggcggatgcggtgctggtaacgagcggtacggcaactttggaggtggcgttgtgtaagcgtccgatggtcatcagctacaagatttcgccgctgacctatgcttatgtgaaacgcaaaatcaaagtgccgcatgtcggcctgccgaatatcctgttgggtaaggaggctgtgccggaattattgcaatctgaagcaaaaccggaaaaactggcggcggcgttggcggactggtacgaacaccccgataaggttgccgcgctgcaacaggatttcagggcgttgcacctgctgttgaaaaaagatacggcggatttggccgcgcgcgcggttttggaagaggcgggatgttgagcggttaatggattattttcccgaagcagcacgtattacaaaaaaagggggagaaattgtgattaatggcacatcaaacaataagtatttaagaggaattccaaatgaaacagaactggcccgaatgggattaaggttaaaatataatggtcagttaactgattaattttgttatatatgatttatgattatagcttatactaatacgcttacttaccttgtttcatttgttcttcgtaaatttctattttaggcaattgtgtcagttcaatagggcaagttgctccccaccaaaaatgttctacataaaaccaaggattatctggaaaatatagcaacatctcttccatatccggccaaattcttcttaattcatctacctgtgtttttggcgaaccagttaatatttttggaggattttcacgataatcgcataattcaataacaccatctgataaaagttcttccaaaaaatcaaaaaatctaatttttaaatttggatctttgatatccatatttaaataatttttataaacaccaaaaataccacctaaatattcacaatattctaaaagattatattttatcttcacattcataacgtaacctttatctaaattttaattctaatctttgcccatgtactgaatcaggttgattcctaaactcaatcgtccattttgctccagtttgttctcggctagttgaaaaattccttaaaataaaggaagagtttaaacaactgaaatttcataagagtagtagaaccaacttggactcaaaaaatcttaaactcattgtttttgaaaaggtacaataatatgacaacttataccattccaaaaaaagattatcaatttctgtatatatatgagggcactctattaaactatactttgaaaaacgatgaattccatatcatcgtccagaatgtggattatccggactttcctcaagagattcctacaccaaattatacagactgggtaaaaattaaattcaagcagttcagctatctgaaatttatctatggatacgccacgaagaaccaagataaaaatatcaaaaatgtattggaacttggagaattaaagcaggatgatgaaatcttggattatggaggtgcgctggaagtgataggcagtaggtatgatcttccgaccggttttagtatagatatagtttgccgggaaatagagttagaatttttagatcaggagagtttcaattaaacgagccgtagcttgttatgctgagcaggcaactttatcgtatttccttttcggttgaaaccccgccactcggacatctgtccttcggggcggtagaatcagattttatttgggaggggcgtaaccccttccgaatcagggcaacacatagggcgacgctttatgtgtcgtcctgtgtgttgaaacattgatatgccgatacggagcctgtcggcaaaatgccgtctgaacaatatcttttcagacggcattttgtatgggggttaacggttgttcagcccgagtacgtcctgcatatcgtacaaacccgttttgccgttgacccaaactgcggcgcggacggcaccggcggcaaaggtcatgcggctgctggccttgtgggtgatttccacgcgctcgccgtcggtggcgaagagggcggtgtggtcgccgacgatgtcgcctgcgcggacggtggcaaagccgatggtcgacggatcgcgcggaccggtgtggccttcgcggccgtaaacggcgcattgtttgaggtctctgccgagcgcgccggcgatgacttcgcccatgcgtaacgcggtgccgctgggggcatcgactttgtggcggtggtggccttcaatgatttcgatgtcgtagccttcgtttaatacgcgtgcgacggtgtcgaggatgtggaaggtgaggttgacgccgacgctgaagttggcggcgaaaacgatgcctgttttttcggcggcagtgtggatagcggctttgcccgtatcgtcgaagcctgttgtgccgatgatgatgttgacttgtttttcaacgcatttttgcaggtgtttgagggtgggctcggggcgggtgaagtcgatgagtacgtcgctttgtgcgagaacggcgtcaacgtcgtctgaaatggcgatgccggttttgagtccgacggcgtagcctgcgtccagcccgagggcttctgagcctgagtgttcaagcgcaccggaaaggacggtgtcgggatggttgttgacggcttcaaccaatacgcgtcccatacggccgtttgcgccggcgatggcgattttgagcggtgtcatgtgtgttccttatggtttgtctgtgttttggcggtctttgagggcttcggcagcgttttgcaggacgtcgccttcggtgcggacgagtacgccgttttcaaaatagacggtcagattgctgcgttctttgatgatgccgttgcgggaggtgttgaaggtatagtcccagcggtcggtatggaatgcgtcgcgcagtatggggctgccgagcaggagcaggacttggtctttggtcatgccggggcggagggcggcaacggcgcgcggttcgagttcgttgccctgtatgattttgagtttgtacgaggggaacagtgaaacgcgttcggcactgcacgcggcaaggccgaggagggcggaaagggcgaggatgagggttttgttcacggaaatgcctttctgtgcaaatcgggatgggtagtgtaacactgcttgaatattttataaaagcgaacgataatcatacgattaagcggtatccgccctgtccgcgcatcggccgccggtgcggttttactattgcaaactgctat

附录Bggtgcgatagtgggcaaacaggccgaaattgcgtattataacgtctattgttttacaggggtattgaatattatggaaaaattcaacaatattgcacaactgaaagacagcggtctgaaggttaccggcccgcgtttgaagattttggatttgttcgagacgcatgcggaagagcatttgagtgcggaagatgtgtaccgcattttgttggaagagggtgtggaaatcggtgtggcgacgatttaccgtgtgctgacccagtttgagcaggcgggcattttgcaacgccatcattttgaaacgggcaaggcggtttatgagttggacaaaggcgaccaccatgaccacatcgtctgcgtgaagtgcggcgaggtaacggaattccacaatcccgaaatcgaagccctgcaagacaaaatcgcggaagaaaacggctaccgcatcgtcgatcacgcgctttatatgtacggcgtgtgcagcgactgtcaggccaagggcaaacgttaaatccggacggtttgttgttcagacggcattcatgattttggatgccgcctgtgtttttggagaactgtcatgcgtattccgctgcttgcccctgacaattatgcctttcccgatcctgcctatgctttggcccggtgcgacgggctggtcggcgtgagcggcgatttggatgcggggcggctgcttgaggcgtatcggaacggcgtgtttccgtggttttcccgggacgggtggtttttttggtatgcggtcgggccccgtgcggtggtgtttcccgacaggctgcatattccgcgctcgctggcgaaaacgctgcgcaacggcagctatcgggttgcggtcaacggctgttttgcggaagtggtcgcgcattgtgcggcagcggcgcgcccgaatcaggacggaacttggattgcgcccgagtttcagacggcatatttgaagctgcacgaaatggggtacgcgcattctttcgagtgccattatcccgatgaaagcggtgaaacgaggttggcgggcggcttttacggcgttcagatcggcagggtgttttatggcgaatcgatgttcgcattacaaccggatgcgtcgaaaatcgcgtttgcctgcgccgtgccgtttttggcggatttgggcgtggaactgatagactgccagcaggatacggaacatatgcgccgtttcggttcggagctgctgccgtttgcggattttgccgaacgtctgcggatgttgaacgccgtgccgttgaaagaggaaatcgggcggcgcgaagtggcgtgcaaggggctttgatggcggcttatgctccggtcaggttcaaatatggtggattatagtggattaacaaaaatcaggacaaggcgacgaagccgcagacagtacaaatagtacggcaaggcgaggcaacgccgtactggtttttgttaatccactataaaattagaaatgacgacagccggataaaatcacggtgaaaatgaaaaatgccgtctgaaacttgaaaacatcgggtttcagatggcattttgtttgacggtttgttgcttatttgagcgggcgcacttcaagtccgaacatacggcgtgcggtgttcagcatttggcagctgaagccccattcgttgtcataccaagcgaacactttgaccatgttgccgtcaacgactttggtcagtgttgcgtcgaagtggctggcttcggtagtgtggttgaagtccatggaaaccaagggcagggtgttgtagcccaaaacgcctttgagcgggcctgcttccgaggcggctttcatcagtgcgttgatttcttcgactgtggtgtcgcgcgcggcttggaagctcaaatctaccaatgatacgttgacggtcggcacgcggatggcaagcccgtcgagcctgcctttcaattcgggcagtaccaaaccgacggcttttgccgcgccggttttggtcggaatcatgttttccacgccgctgcgggcgcggcgcaggtctttgtggcgcacgtcggtaacggtttggtcgttggtcagcgcgtggatggtggtcatcgcgcctttgacgatgccgacgctttcgctcaacactttggcaaccggcgagaggcagttggtggtgcaggaagcgttggaaacgacggtcatgtcggcggtcaggacgctgtcgttcacgccgtacacgacggttgcatcgacatcgtcgccgcccggtgcggaaatgaggacttttttcgcgccgctttcgaggtggattttggctttttctttgctggtgaacgcgccggtgcattccatgaccaaatcgacaccgagttctttccacggcagttcggcagggttgcgggtcgagaagaaggggattttgtcgccgttgacgatgaggttgccgccgtcgtgggatacgtcggcttcaaagcgtccgtgcacggtgtcgaatttggtcagatgggcgttggtttcaaggctgccgctggcgttgacggcgacgatttggagttggtcttgaatctgataatcgtagatggcgcgcaaaacctggcggccgatgcgtccgtagccgttgatggcgactttgatgcccatggtttgttcctttgttgagggttgggtagattttcggggcggattatagcaaatttgtagtggcgtgtaattaatattttattgaaaacggcgcggccggaagggtgggcggtaagatgcggacggcacgggtgcggcggacggagagcttgataaaatgccgtctgaagcggcttcagacggcatatcagggaagggtcaggaggcggtattctgtgcggcttcctgtttggctttgtattgtttgagatattcgagggcggcggctttttcgctgtcgctgccgtatttcatatcgcgttgggcgcggcgcaactcggcgcgttcgcgggcttcggctatctgtttcgcctgatagtctttgcggttgtcggcggcggcgaggcggtcttgttgggtttgcgcttttgccatggctttggcgatgaggtcggcagggttaaacgtcggttttttcggtgtgtcgggcgtttgcggacgcgcgttgcggacggcggcttcgcgttcggcaagcatggccttgcgttcgtcggcttcgcgctgtttgcgttcgttgcgtttgaggtagcgcgtgcgcgcgtgttcggcggcggcaaaacggctgtcggcggacaggctgaagcggcgcgcgcggggcaggacggtgtcggcaacgggctgcatatggatgcagtcgacggggcagggggcgacgcagagtccgcagccggtgcattcgtcggcgatgacggtgtgcataagtttgcccgcgcccataatggcatcggcagggcaggcgcggatgcaggcggtgcagccgatacaggcggtttcgtctatccgggcgagtgctttggcttgggttttggcaggtgcgacaaagggtttgccgagcagggcggaaatgtcccgaatgacggtttctccgcccggggcgcagaggttgtacgcttcgcctgttgcgactgcctgtgcgtagggcaggcagccgtcgtagccgcattcgcggcattgggtttggggaagcaggcggtctatggcggcggctgtggcggtcatgtcggtgtgcggctcaaaatcgaaagggcgtattttagcagaattgtatgccgcgcccgtttcggatggtgcgcggtgttttgttataatgcggcggcgtatgccgtttcagacggcatttttctgtattttcctgttcggacggtctatgaacgaattttcgcttgcccctattgtgattgttttgctggtgtcggtcattacggtgatcctgtgccgcaagttcaacattccctccatgctgggctacctgctggtgggctttttggcggggcccggtatgctcagcctgattccgaaaagccatgcgacggattatttgggcgaaatcgggattgtgttcctgatgttcagcatcggtttggagttctcgctgcccaagttgagggcgatgaggcggctggtgttcggtctgggcggtttgcaggtcggcattacgatgctgtcggtaatgggcatactgatgctgacgggcgtgccgttcaattgggcgtttgccgtgtcgggcgcgttggcgatgtcgtccacggcgattgtgagccggattttgtcggaaaagacggaattggggcagccgcacggtcagatggcgatgggcgtgctgctgatgcaggacatcgccgtcgtgccgctgatgattctgattcccgcgctggcgggcggaggggacggaaatatttgggcggccttgggtttggcgtttgcaaaaatgctgctgacgctggggctgctgtttttcgtcggcagcaaaattatgtcgcgatggttcaggatggtggcaaaacgcaaatcgtccgaactctttatgatcaatgtgctgctggtaaccttgggtgtggcttatctgactgagctggaaggtttgtctatggcgttgggcgcattcgttgccggcatgctgctttcggaaacggaataccgtttccaagtcgaagacgacatccgcccgttccgcgatattttgctcggctttttctttatcacggtcggcatgaagctggacattcaggcattgatcggcggctggcggcaggtattgatgctgttggcaatgctgctggtgttgaaggcactggttgtgtttgccattgccttcaaaatgaaacattcggtcggcgacagcctcaaaacggctttgtatctcgcgcagggcggcgagttcggcttcgtgatgctggccattgccgggcagcttgatatggtttcgccagaatgggaacaggcggcgacggcggcggttctgctgtcgatgattatcgcgcccttcctcttgggcggcagcgatgcgctggtcgggcgtttggtcaagtcaagctgggacatgaagtcgctcgatctgcacagtatgctggtagaaaccatgagcaagtccgaccatgtgctgattgtcggcttcggcaggggcgggcagacggtcggacgcgtccttgcccaagaggatattccgtatttcgcgctcgacttggacattgcgcgggtgcaggttgccagaagtgcgggcgaaccggtgtcgttcggcgatgcgaaacgcagggaagtattggaagccgccggtctgggacgggcgaaaatggtggtggttacgctcaacaatatgcacgaaacgcaacacgttttagacaatgtgctgtccatgtatcccaatatgcccgtatatgtgcgcgccaccaacgacgattatgtgaaaacgtttaccgatataggtgcggaagaagccgtgtcggacaccaaagaaaccggactcgtgctggcaggctatgcaatgttaggcaacggcgcgtcgtatcggcacgtctatcagacgatggcaaatatccgccacagccgttatgccgcgttggagggactgtttgtcggtagtgatgatgaggcaggattcggcgaaaacggcgaaaccgtccgtcacgcctttcctttggctgcagaagcatacgccgtcggcaaaacagtcggcacgcttccgatggcggcttacggcatcaaactcttgttcgtccgccgccgcaccggccggattgaaaacccggatgcctcgtttacattggaaggcggtgacgtgttggtggtcgcaggcaaaaaagaagaaattatctcttttgaaaactggagtttgcagggaatataaatgaaatgccgaaataaggcttgcgccatttccggttatttggtttaataacgctttcgcaaatcgcaagggtgattagctcagttggtagagtgtctgccttacaagcagaatgtcggcggttcgactccgtcatcacccaccaagttttctttcattgttgcaaacaatggatgcgcggtggtagctcagttggttagagtaccggcctgtcacgccgggggtcgcgggttcgagccccgtccgccgcgccaagtttcaaaatactgactctgtcggtattttttatacacgggtgattagctcagttggtagagcgtctgccttacaagcagaatgtcggcggttcgactccgtcatcacccaccaagttttctttcattgttgcaaacaatggatgcgcggtggtagctcagttggttagagtaccggcctgtcacgccgggggtcgcgggttcgagccccgtccgccgcgccaaaagttaaggaataccaacctccggttggtatttttttgtttgtatgctttgaaaaatgtttgtttccggattttgccattcccatccggttttgcgctgtacgatgtgttttagcgcggacttgctcaaaatcgcatgtgattccggtatttgaggctttgattagggatgcggactttcaatatattttctcagctacaacaacgaaggcttgatgtctgtcgggcaggtaagggagatttttgagcgtttcggcaaatataatttggttcaaacggaataccggcgttttaaggcagataagacagaaaaccgtaatcataaggcaaattcgatattcgaatttctgcatattttagaaaagaccttttatagtggattaacaaaaaccagtacagcgttgcctcgccttagctcaaagagaacgattct

附录Bctaaggtgctgaagcaccaagtgaatcggttccgtactatttgtactgtctgcggcttcgtcgccttgtcctgatttttgttaatccactataaaaattcttgccggatgctgcaaacaacgccggtttgcattcctgatggcggtggttttcttagacgaacgcccgaacacgcaggaatggataggcttggggctggttacggcgggcgtgttgacgctggcactgaaacggtaaagccgcaagaaataaatgaaatgccgtctaaaaaactgttttcagacggcattttcgtttctgtccatcctcagcactcgaccacgcgcacggatacggggacggcttttttccggagcgtggcggcgagtccggcaagcgaggtttctttgtaggtcgccttcatatcccggccggtttgcagcatggttcggatgacttcgtcgagcgagacttttttgtccgtgccgtcttccaaaagcgcgagcgtgccgagtttgagggctttttcggcggcgatgccgttgcgctcgatgcaggggatttgcaccagtccgccgacggggtcgcaagtcagccccaaatggtgttccatcgccatttcggcggcgttttccacttgtttgggcgtgccgccgatgacttcggcgtatgcgcccgccgccatcgaacacgctacgccgacttcgccctgacagccgacatccgcaccggaaatggaggcgttggtcttgtagaggatgccgattgcgcctgcggtgagcaggaagttttcgacgcgttcctgtgtggcgtgcggattgaacttgcggaaatagtgcaatacggcgggaatgatgcctgccgcgccgttggtcggtgcggtaacgacgcgtccgccggcggcgttttcttcgttgaccgccatggcgtacaccatcggccagagctgggtgttgacgatttcggtttcgcgcaggactttgagcttggcggcaagctgcggggcgcggcggcggacgttcaatccgctgggcagttcgccgtccgcacccaagccgcgtttgatgcagccttccataacctcggcaacggcagcggcgcggcggcggatttcggcttcgccgcatccggcaagcgcggcttcgtttgccaacacgacttcggagatgtcgagccggttcagacggcatcgggcaagcagttcggcgcaactggtatagggatagggaacggctttttccgtttccgcctgccggtcaaaatcttcttcggtaacgacaaagccgccgccgaccgaataataaacctgttcattcaataccgtgccgtctgaagcataggcggtaaaacgcaggctgttggggtgtttgggcagcacttgattgccgagtatgttcaggtcgcggtcggggatgaagcggatttcttgcccgttgagccggaggatgtgctgcgtgcggatgcgttcgaggcgttcgggaatgccggcaagcgggatgtcgtgcggcaggctgccttccaaaccgagcatcagcgcgtcaaatgtaccgtgtccgtatccggtcagtgcgagcgagccgtaaatgtcgatgacgatgcgaacagcctgtgcatccaaacctgccgcaaaggcggcggctgccttcatcgggccgaccgtatgcgaactggaaggcccgataccgattttgaaaatatcgaaaatgctgatcatattttgctccgacggtttttcagacggcacaggttccgtttgaccaaccaaaaaggagacgcggcacgatgcccgtctccttttttaaaacggcacttatgcgtcgatattttgggcaatcagcgcgttgttttcgataaaggcacggcgcggctcgacctcgtcgcccatcagcgtaacgaacacttcgtcggcggcaatggcatcttcgatgcgcactttcaacaggcggcgcacggcgggatccatcgtggtttcccacagctgctcggggttcatctcgcccaagcctttgtatcgttggatggacatacctttttgggcaacgctcatcaagatgtccaaagcggtttcaaagctgtccgcgtcgtacccgttttcgcctttgtaaagcttggcaccctcgccgaccatgcctttgagcgcggcggcggtttgggtgagggtttggtaggctttgctgttgaggaacttgggttcgatgtagctgaccatgacgttgccgtgcagcttgcgcgtgattttgatgaaccggtgtccttcatgaccttcgatgcgttcgagggcgacttctttttcgtcaagcagaccggaaagttcggcaacggctttatcggcgttttcagacgacgtcaaatcaatgggcgacgcgtgtagcatggcgcgcaggacgagttcgtctacgaagcggctttcctgttcgatgacggtttttgccaacaggaattgtttggcggtgtcggcaagttctgcgccttcgatggtgcggccgtctgaaatgattttggctttttccaaggcaagaccgagcagccattggtctttttccaactcgtccttgaggtaacgttcctgtttgccgtatttcgctttatacaaaggcggctgggcgatatagatgtagccgcgctcgaccagctcgggcatttggcggtagaagaaggtcaggagcagggtgcggatgtgcgcgccgtccacgtcggcatcggtcatgatgatgatgcggtggtaacgcagtttttcggcattgaattcttctttgccgatgcccgcgcccaaagcggtaatcagcgtggcgacttcttggctggccagcattttttcaaaacgtgctttttcgacgttcaaaattttacctttgagcggcaaaatcgcttggaatttgcggtcgcggccttgcatggcggaaccgcctgcggagtcgccctcgacgaggtagagttcggacagggcagggtctttttcttggcagtcggcgagtttgccgggcagtcccaagccgtccatcacgcctttgcggcgggtgatttcgcgtgctttgcgggcggcttcgcgcgcgcgggcggcatcgacgattttgccggtgatgattttggcttcgttcggattttcttcgaggaagtcggtcagggcttggctgatgacttcgttgacaacggggccgatttcgccggaaaccagtttgtctttggtttgggacgagaatttggggtcgggcagtttgacggacaacacgcaggtcaaaccctcgcgcatatcgtcgcctgcggtttccactttggcttttttggcgacttcgttggcttcgatatagttgttgatggtgcgggtcatcacttggcgcagtgcggtcaggtgagtaccgccatcacgttgcgggatgttgttggtgaaacactgcacgctttcttgatagctgtcattccattgcatcgcgcattcgacgctcatgccgtctttttcgccgaacgcgtagaagattttttcgtgcaacggcgtttttttgcggttcatgtattgcacgaaacccgccacgccgccggaaagggcgaagctttcgtgtttgccgtcgcgctcgtcggtcaattcgatgtccacgccgttgttcaggaaggaaagttcgcggatgcgtttggcaaggatgtcgaagctgtattcgacgttgccgaaggtttccgtactggcgaggaagcgcacggtcgtgccttttttatcggaatcgccgacaattttcagcggctcttcggtttcgccgcgcacgaagcggacgaagtgttctttgccgtcgcggtagatggtcagcgttacccagtcggacagcgcgttgacgacggacacgcccacgccgtgcaggccgccggagattttgtagctgttgttgtcgaatttaccgcccgcgtgcaatacggtcatgatgacttcggcggcggagcgtccttctttcgggtggatgccggtgggcataccgcgcccgttgtcggcgacgctgacggaatggtcggcgtgtatcgttaccgtgattttgtcgcaatgtccggcgagtgcttcgtcaatggcgttgtccaatacttcgaacaccatgtggtgcagaccgctgccgtcctgcgtgtcgccgatgtacatgccggggcgtttgcgtaccgcttccaagccttcgagcacctgaatgctgtcggcgccgtattcttcgtgtttttgttcagtcatattttttgccggattttgaaaagattttgcgatgccgccaaaacaagtccgcaccttgtagaaaaagcgggcgggacgacatatataattgtgtattatagccgattttgccgcctaattcagcgttatccgcatcagtgtgccgccgggaaaagatgaaacggtacgtttgcctccggcatcaggtcggggattgtcccgtaaagtggcaaaagcgtttttttgccactaaaatctacaccctatacttttcggacaggggcgcggaaatggaaatatggaatatgttggacacttggctcggtgccgtcccgatacgtgcggaggcggtcgaatccgtggcggcggttgcggctttgctgctggcgcgcgcccttctgttgaatatccacttcaaacggcatccggatttcggcatcgaaagcaagcggcggtttttggttgccagccgcaatataacgctgcttttggtgctgttttcgctggcatttatctggtcggcgcaaatccaaacgctggctttgtcgatgtttgcggtggcggcggcggtcgtcgtggcgacgaaggaactgattatgtgtctgtcgggcagtattttaaggtctgccacccagcaatactcggtcggcgactatatcgaaatcaacggcctgcgcgggcgcgtggtcgacatcaacctgttgaacacgctgatgatgcaggtcggtccgaaccccttggtcggacagcttgcgggaaccaccgtttctttccccaacagcctgttgttgagccaccccgtgcgccgcgacaatattttgggcgactatgtcatccatacggtcgaaatccccgttcccatccatttggattcggatgaagccgtatgccgtctgaaagccgtactcgagcccttgtgcgcgccctacatccccgccatccaacggcatttggaaaacgtgcaggcggaaaaactgtttatcacgcccgccgccagaccgcgcgttacccgcgtgccgtacgatgacaaggcataccgcatcatcgtccgcttcgcttcccccgtttcaaagcggctggaaatccaacaggcggttatggacgaatttttgcgcgtacaataccgcctgttaaatcaccccgccggctccgaaacactttaactttccccgaccgaccccatttccggcttcagacggcatattgccgatatgctgtctgaaacacaacacgcaaaggaaacccatcttatgactgacaacgcactgctccatttgggcgaagaaccccgttttgatcaaatcaaaaccgaagacatcaaacccgccctgcaaaccgccatcgccgaagcgcgcgaacaaatcgccgccatcaaagcccaaacgcacaccggctgggcaaacactgtcgaacccctgaccggcatcaccgaacgcgtcggcaggatttggggcgtggtgtcgcacctcaactccgtcgccgacacgcccgaactgcgcgccgtctataacgaactgatgcccgaaatcaccgtcttcttcaccgaaatcggacaagacatcgagctgtacaaccgcttcaaaaccatcaaaaattcccccgaattcgacaccctctcccccgcacaaaaaaccaaactcaaccacgatctgcgcgatttcgtcctcagcggcgcggaactgccgcccgaacagcaggcagaactggcaaaactgcaaaccgaaggcgcgcaactttccgccaaattctcccaaaacgtcctagacgcgaccgacgcgttcggcatttactttgacgatgccgcaccgcttgccggcattcccgaagacgcgctcgccatgtttgccgccgccgcgcaaagcgaaagcaaaacaggctacaaaatcggcttgcagattccacactacctcgccgtcatccaatacgccgacaaccgcgaactgcgcgaacaaatctaccgcgcctacgttacccgcgccagcgaactttcagacgacggcaaattcgacaacaccgccaacatcgaccgcacgctcgcaaacgccctgcaaaccgccaaactgctcggcttcaaaaactacgccgaattgtcgctggcaaccaaaatggcggacacgcccgaacaagttttaaacttcctgcacgacctcgcccgccgcgccaaaccctacgccgaaaaagacctcgccgaagtcaaagccttcgcccgcgaaagcctgaacctcgccgatttgcaaccgtgggacttgggctacgccagcgaaaaactgcgcgaagccaaatacgcgttcagcgaaaccgaagtcaaaaaatacttccccgtcggcaaagtattaaacggactgttcgcccaaatcaaaaaactctacggcatcggatttaccgaaaaaaccgtccccgtctggcacaaagacgtgcgctattttgaattgcaacaaaacggcgaaaccataggcggcgtttatatggatttgtacgcacgcgaaggcaaacgcggcggc

附录Bgcgtggatgaacgactacaaaggccgccgccgtttttcagacggcacgctgcaactgcccaccgcctacctcgtctgcaacttcgccccacccgtcggcggcagggaagcccgcctgagccacgacgaaatcctcatcctcttccacgaaaccggacacgggctgcaccacctgcttacccaagtggacgaactgggcgtatccggcatcaacggcgtagaatgggacgcggtcgaactgcccagccagtttatggaaaatttcgtttgggaatacaatgtcttggcacaaatgtcagcccacgaagaaaccggcgttcccctgccgaaagaactcttcgacaaaatgctcgccgccaaaaacttccaacgcggcatgttcctcgtccggcaaatggagttcgccctctttgatatgatgatttacagcgaagacgacgaaggccgtctgaaaaactggcaacaggttttagacagcgtgcgcaaaaaagtcgccgtcatccagccgcccgaatacaaccgcttcgccttgagcttcggccacatcttcgcaggcggctattccgcaggctattacagctacgcgtgggcggaagtattgagcgcggacgcatacgccgcctttgaagaaagcgacgatgtcgccgccacaggcaaacgcttttggcaggaaatcctcgccgtcggcggatcgcgcagcgcggcagaatccttcaaagccttccgcggccgcgaaccgagcatagacgcactcttgcgccacagcggtttcgacaacgcggtctgacggcagggttgaagtaaaaaatatggcggattcgatagaaaaacatccgcaccgtcattcccgcgcaggcgggaatccagaccggtcggtgcagaaacttatcgggaaaaacggtttctttagattttacgttctagattcccactttcgtgggaatgacgcggaaaagttgctgtgattccggataaattttcgcaacgtttaatttccgttttacccgataaatgcccgcaatctcaaatcccgtcattccccaaaaacaaaaaaatcaaaaacagaaatcccatcattcccgcgcaggcgggaatccaggtctgtcggtgcggaaacttatcggataaaacggtttctttagattttacgttctagattcccgctttcgcgggaatgacggaatatttttgaatttgataaaaatgccgtctgaaacggtcaaacaacgcttcagacggcattttatagtggattaacaaaaatcaggacaaggcgacgaagccgcagacagtacaaatagtacggaaccgattcacttggtgcttcagcaccttagagaatcgttctctttgagccaaggcgaggcaacgacgtactggtttttgttaatccactatattttccgacatcattgaatcaaacccaaatgcgacaagagcgtccatgtgccgatggcaatcaacaccaaacctccggcaaattccgcacacctgccgaacaatacgcccaaagcccttcccgccgtcagcccgaccgccaccatcaccgtcgtcgccataccgatgattgcggcggcaaaggcgatgtttacctccataaacgccaagcccaccccgactatcatggaatcaatactggttccaaaagcagtcaaaaccgtcatccataggctttcccgtttgctttcgcgcacatcttccgcctcgccggacagcccttcgcgcatcattttcagacccagcccgcccagcaggacgaaagccacccaatggtcccattcgctgataaacggcttggcataaaaaccgcctacccagcctgccagcggcgtgagcgcttcaaccgtgccgaacaccaaagccgttgccgcaattttgcgcggaggcattctgaccgccgcaccctttgccaatgcgacggcaaacgcatccatcgacatccccagagcaatcaagagcaaagcataaaaacccataccgcacccgtcctcaaaaagggcggattatagcaaaagcaaaaaaatgcaaaaatgccgcacgaaaacccgcatcccgtcattcccgcaaaaacaaaaaatcaaaaacagaaatcccgtcattcccgcgcaggcgggaatccagagttgtcggtgcggaaacttatcggataaaacggtttctccaaccccgagtccttgattcccactttcgtgggaatgacgggatattttgcgtttaataaaaaacgcccgctgaaacggcggggcgggatgggggaatgccgtctgaaacggtcggacaatgtttcagacggcatttttatgcccggttatttccgatagcggacggcgcgggacaggatttcttcaatttccatccacataatgcccccttacagcaaaccagcctgacccagtgcgggatcggtcgcgcgggcggcttgggcatcttcgacagtcagtccaagggctttggccacgccttcgccgtatgccgggtcgcaacggtagcagttgcggatatggcggtatttgatgaagtcgggcgcgtcgcccattgcggcggcggtgttgccgaacaatgcctgtttctgcgcgtcgttcatcaggttgaacagggcgcgcggttggctgaaatagtcgtcatcgtcttggcggtagtcccagtgtgccgcgtcgccgttgattttcaaaggcggttcggcgaagtcgggttgttgctgccattggccgaagctgttgggttcgtagtgcggcaggctgccgtagttgccgtcggcgcggccttgcccgtcgcgctggttgctgtgaacagggcaacgcggacgattgacgggaatttggcggaagtttacgcccaaacggtagcgttgtgcgtcggcgtaattgaacaaacgcgcttgcagcattttatctgggctggcgccgacaccgggaacgaggttgctcggtgcgaaggcggattgttccacatcggcgaagaagttttcgggattgcggttcaactcgaattcgcccacttcaatcagcggatagtcttttttcggccaaactttggtcaagtcaaacggatgataaggtactttttccgcgtctgcttcaggcatgacttggatgtacatcgtccatttcggaaactcgccgcgttcgatggcttcgtataagtcgcgctgatggctttcgcggtcgtcggcgatgattttggcggcttcttcgttggtcaggtttttaatgccttgttgggtgcggaaatggaatttcacccaaaaacgctcgcctgcttcgttccagaagctgtaggtatgcgaaccgaagccgtgcatatggcggtagccggcggggatgccgcggtcgctcatcacgatggtaacttggtgcagtgcttcgggcagcagcgtccagaagtcccagttgtttgtggcagagcgcatattggtgcgcgggtcgcgtttgacggctttgttcaggtcggggaacttacgcgggtcgcgcaggaagaacacgggcgtgttgttgccgaccacatcccagttgccttcttcggtataaaatttcaaggcaaaaccgcggatgtcgcgttctgcatcggctgcgccgcgttcgcctgccacggtggtgaaacgggcgaacatctcggtttttttgccgacttcgctgaagattttggcgcgggtgtatttggtgatgtcgtgcgttacggtaaacgtaccgaacgcgcccgaacctttggcgtgcatacggcgttcggggatgacttcgcgcacgaagtcggcgagtttttcattcagccacaaatcctgcgccagcagagggccgcgaggaccggcggtcaggctgttttgattgtcggcaacaggcgcgccgttgttcatggtcagatgggttacagggcatttggaggtagtcatcgctcttgttccttttctcaggttggtcaaatgggggtaaacggcttacagtacgatttggcggaaagcgtattcgtaaccggtttcttgattgcaataaatttcttgaatcgacattttatttcccttttgtaaaaactatggatgcgactatacgccaagattttcgctattaaaactatgaaatcgatttaatattattataagcaatcggttcttgattttcgtttgttttttgttatcgaacggaatccgaacccgctcattaaaaccatttataatgcaatgacgctttgcggcattttttgcgccgacaggctgaaaataacaattttccccacattatcatgaccttactcggaataaagctcaaacagacccagcagctcaaccagcggctgcaacaatctttgcgcgtattgcagatgtcgggtatcgaacttgaacgcgaggtcgaaaactggctgtcggacaaccccctgctcgaacgcaaagacacggatgaattttccgatgccgagttcagccattacactgcgcctgcccgtcaaatcggcggagacgaaggcgaagatatgctgtccaacatcgccggcgagcaggatttcaagcaatacctgcacgcgcaagtatgcgaacacccgctttccgaccaagaagccgcctgtgtccacatccttatcgatttccttgacgagcagggttatctgaccgacagcatcgaagacatcctcgaccatacgcccttagagtggatgttggatgaagcaatgctgcaacacgcgctgaccgcattgaaaaaattcgacccggcaggcgtggccgccgccgatttgaacgaatcgctgatactgcagatagaaagattgggcgaatgtgctgccaaacccgccgccctgcatatcgtccgaaacgccctcgacagcattgacggcaaccgcagccaaaccctcgcacgaataaaaaaacacctgccccaaaccgacagcggcacactcgaagccgcactcgacctcattgcttcgctcaatccctttcccgccgccggttttgcctcgtccacgcccacgccgtattctgacgaggcgctcgccaacctgctggctttccgcggcatggaggtttctcgccgcaccattgccaaatacagagaatcctttgagattccggcagcacacaaacgcaaaaccgcagaataattgccgaataatcttataaagacaacaaaccaaaagccggcatttctgcgaaagcgggaatgccgaatccgtccgcgcggaaacctgcatcccgtcattcccgcgaaagagggaatctagaaacgcaaagctgcaagagtttatcggaaatgaccgaaactcaacgaacctggattcccgctttcgcgggaatgacgggggtttggcgggaatgacgagggtttgggatttctgtttttgaatttctgtttttgtgagaatggcaagattttcggttcttgtatggataacgagattttagatggcgggaatttgtcgggaaaacagcaatctgagacctttgcaaaaataatctgttaacgaaatttgacgcataaaaatgcgccaaaaaattttcaattgcctaaaaccttcctaatattgagcaaaaagtaggagaaatcagaaaagttttgcattttgaaaatgagattgagcataaaattttagtaacctatgttattgcaaaggtctcaatctttaccgtcattcccacgaaagtgggaatctagaaacgcaaagttgcaagaatttatcggaaatgaccgaaactcaacgaacctggattcccgctttcgcgggaatgacgagggtttgggatttctgtttttgaatttctgtttttgtgagaatggcaagattttcggttcttgtatggataacgagattttagatggcgggaatttgtcaggaaaacagcaaccctccgccgtcattcccacgaaagtgggaatctagaaacgcaaagttgcaagaatttatcggaaatgaccgaaactaaacgaacctgaattcccgctttcgagggaatgacgggggtgtggcgggaatgacgggggtttatcagaaatgaccgaaactcaaaagcgggcagccttgtttacgccttcaaaatatcgagcaatttcaaatcgactttttcggcatcgaatttatctttggcaatcgcataacttgcattccccatcaggcggacggcttccctgttttcgataaaataaatcattttttcggccaagatgcggggattccaaggctcgatcaggaagccgttgaccttgtcggcgaccgtttccctgcatccggggacatccgtcgtaatcactgccctgccgacggccattgcctcctgagtgcttcggggaacgccttccctataataagacggcaatacgaatatatgatgttcttttatcacttcggaaacattgttcacaaaaccggggaaacggataatatcgcgggcggcaagccgttccaaatcgcccccccccccgcgtgatttgtcgattgcgcccaaagcggtaaaaaccgtatcggggtatttgtccttaacctgttccgccgcccgaataaaatcatcaatccccttttctttcagaaatctgccgataaagaggaattttacgggttctttttcatcgggaatatccgcctcggaataaggatattgccgcaaatccagaccgattccgcccaaaatatggatgttttttattttgatgccgtatttgtccgtcagttcgtctttgtcgtc

附录Bggggtttaatacaatcaggctttccaacatcggcagggcaatgcggtataaggcaatcaaaatcccctttatgatttttgtttttaacggtatgccttccggctgcggggtaaatgcgaatcccaaaccttccagcatcccgacgattctgggcacgcctgccagttttgcggcaaaagtgccgaaaatcacgggttttgcgaaataagggaaaaccaaatccggcgatatttttttgagttctttaaagatgaggaaggtggattttatatccgaaaacgggttcagcccgctgcggtttgaacggtaggtaacgggtgtaacccccatttccctgataatatccaattcattgtcggaaaactccgatacaaaggcatacacctgatggtttttgccgattaattttttaatgacgggggcgcggaaaccgtaaatgctggatgcgactgttgtgataaaaacgattttcataaggcggacaccttgaatatggattggaaatgcggtctgctacggcagggtttcatcctgtaacccagcaaggcttgggtttgcctgcgtattatagtggattaacaaaaaccggtacggcgttgccccgccttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttccgtactatttgtactgtctgcggctcgccgccttgtcctgatttttgttaatcactataaaaatgccgtctgaaacggtttcagacggcatttcgatgtcggcggcggctttgcggaatcagcctttgaagcgtttgaagaccagcgtgccgttggtgccgccgaagccgaaggagttggaaatggcaacgtcgatttccgcgtcgcgcgcttcgttggcgcagtagtccaaatcgcagccggcttcaacgtcttgttcaaaaatgttgatggtcggcgggattttgccgtcgtgtatcgccaaaatgctgtacacggcctccacgccgcccgccgcgccgagcaggtggccggtcatggatttggtcgagctgacgacggttttgtaggcgtgttcgccgaacgcgcgtttgagggctttggtttcgttggcatcgcccaagggggtggacgtgccgtgcgcgttgacgtaatccacgtcttcgggattgatgccggcatctttcagcgcgcgggtaacggcaagggcggggccttcttcgttcggcgcggtgatatggtaagcatcggaactcatgccgaagccgacgatttcggcgtagattttcgcgccgcgttttttggcgtgttccaattcttccaacaccaatatgcccgcgccttcgccgataacgaagccgtcgcggcctttgtcccacggacgggaagcggtggcggggtcgtcgttgcgggtggagagggctttcatcgcggcaaaaccgcccacgcccaaagtgctgattgcgccttccgcgccgccggcaaccattatgtccgcgtcgccgtatttaatcatacggagggaatcgccgatggcgtgcgcgccggtggtgcaggcggaaaccatcccgtagctcgggccgcggtagcctttgaggatggtaacgtgtccggaaatcagattaatcagagaaccggggataaagaaagggttgattttgcgcgcgccgccttcgattacggctttgccggtgacctcgatgccgggcagtccgccgatgccggaaccgatgttcacgccgatgcggtctttgtcgaggttttccacatcgtccaaacccgaatcggcgattgcctgcaatgcggcggcaatgccgtagtggatgaatacgtccatccggcgcgcttctttcgcgctgatgtattgtccgatgtcgaaaccgcgcacctcgccggcgacacggctgttgatgtcggatgtgtcaaagcgggtaatcgcgccgatgccgcttttgccggtgagcagggtgtcccaagcctctgcgacagtgttgccgacaggggaaacctgacctaagcctgtaatgactactcttctctgactcatgataacctcgctgttggttgtcggaatgggggcatatgcggctgtcgtgcagatgccgtctgtaatttgcggcaggggttcaaacagtttgccatataagggaaaagcctctattgcgcggtgcagcagaggctgttgtgtcgggcgacgaccggttagccgttgtgggcattgatgtagtcgatagccagttggacggtggtgattttttcggcatcttcgtcggggatttcgcagccgaatgcttcttccaaagccataaccagctccacggtgtccaaagaatccgcgcccaagtcgtcttggaaggaagattcgtttttcacgtcggcttcgtttacgcccagttgttcagcaacaatttttttaacttgttgttcgatgtttgacatatcagtcgttcctttatgccttgcggcaggttgtttaagggaaataaatcggtggtattgtaccgacttttaatagagttttctatctaatgactattatatcaatatttgccgatttgtacatttttgggtgcggcgggttttgtcgttcaagtttgacctgtgtgccgtatgtttggcgggatttcggttaaaatggcggcatttccatctgaagcagaaagccctgtcatgtatccacttgcccgtcgcatcctgtttgcactcgatgccgaaaaagcccaccacttcacgctcgacgcgctctacacggtttataaattgggtttgattcctgtaaccgacaaccgtaccaaacctgtaaaattgatgggtatggatttgcccaaccctgtcggacttgccgccggactcgacaaaaacggcgaatacatcgacgcattgggcgcgctcggctttggtttcatcgaaatcggcacggtaacgcccaacccgcagcccggcaacccgcagccgcgcctctttcgcgttcccgaacaccaaggcatcatcaaccgcatgggtttcaacaaccacggtatcgacaccatgatacgcaacatcgaaaaaagtaaattcagtggcgtattgggcatcaacatcggtaaaaacgcggttacacccatcgaaaacgctgccgatgattatttaatctgccttgaaaaagcctacgcacacgcaagttacattaccgtcaatatttcctcgcccaacactaaaaacctccgcgcgctgcaaggtggcgacgagttgagcgcattgcttgaggctttgaaaaacaaacaggcacagcttgcctctgtacacgggaaatacgtcccgctcgccgtcaaaatcgcccccgatttggatgaagcacaaatcgaagacatcgcccacgttgtcaaatccgtcgaaatggacggcatcatcgctaccaataccaccatcgacaaatcaagtctcggcagccatccgctcgcaggcgagcagggcggtttgagcgggctgcccgttcatgaaaaaagtaatcgggtgttgaagctgttggcagaccacatagacggcaagctgccgattatcggcgtaggcggcattatggaaggcgaggactcggcagataaaatccgcttgggcgcgaccgccgtccaagtgtacagcggattgatatacaaaggtccggcattggtcaaagaatgtttgaaggctttggcgcgatgacgcgatccgcccaaaatgccgtctgaacgcacgttttgccgttcagacggcattttcatttcctttttccgcctgacgccccttgaaaatcccttacgcgccgccctgtttgaaataaggcaaaccgatgcgtgaacacggagcaggcaatcggagtaaaaaatgaaccttgatttaaccgcgcaaaaagtccgtctttcttggaaggatattctgtgggggtatgggaataaatacttgggttgggctgatgtggcagcttatgcccgaaaaatgacgctttcagatcatgatgaacgtgtgttcaaactatctttaatcaacaaatccaatattcttgaattaaagcctgttctggaagatttggcttcggaaatgagggattattcccctaaaaattggctgtacgtcctcttaagcgatgtattccatagaaaagaagaatttgaggatcctttgggggaagttgaaaaaatttatgcagattttgattatccggaagaaatagaatcatttgtcaggtatatgccgcccaaagacggttatattccttctgcccacacctatgaagaaaatattgcccggttatattctcactgggaacactatttgaacaacggcggagggcagggttaaaaccggcaatccgatgccgtctgaagcattatccggccttcagacggcattttgttttccgacagtttataaactgtcgttgtttcttgacagaaacaacgaccttatttgaaacgattggaggacatgattatgggtttttggaatggtgtggcaaaagcagcaaaagcagtgggagagggaatgattgaagccggcaatgagcataaggcgttgaaaatggaatatgcggagaaatcaagtgaggagctgcatgaaatcgtcaagagtgatggtttttttaaaaattccacacgggagaaaagtgcggcttatgctattttaaaagagcgtggcgaggtgtgaacaggaaacggcggcatttgccgctgttttttattggtaggcatccgtccgaatatcggggcaaggtttcagacgacatcgaaggttgctatgatatagtggcttgactttaaaccggtacggcatcccctcgccttgtcctgatttaaagttaatccactatctcattcccgtcatccttccaaacggaatccgaaatgtccgacaaccgcctcgacaccgcccgccgccattccctcttcctcgcccgccagctcgacaacggcaaactcaagcccgaaatattcctgcctatgctcgacaaggttttgaccgaagcggatttccaagcctttgccgactggggcgaaatccgcgcggaagaaaacgaggaagaattggcgcggcagttgcgcgagttgcgccgttatgtggtgtcgcagattatcgtgcgcgatatcaaccgtatcagcgatttgaacgaagtaacccgcacgattacgctgtttgccgattttgccgtcaataccgcgctggattttgcctacgcctattatcgggacatgtacggcacgccgatcgggcgttataccaaatcgccgcagcatttgagcgtggtggcgatgggcaaggcgggcggctatgagttgaacgtgtcttccgacatcgatttgattttcgtctatcccgaatcaggcgacaccgacggcaggcgcgaacggggcaatcaggaatttttcaccaaagtcgggcagaaactgattgcgctgctgaacgacattaccgccgatgggcaggtgttccgcgtcgatatgcggctgcggccggacggcgattcgggcgcgttggtattgagcgaaaccgcgctggagcaatatttgattacacaggggcgagaatgggaacgctacgcgtggtgcaaaggtcgcgtggttacgccgtatccgaacgacatcaaagcactggtgcgcccctttgtgttccgcaaatatctggattacggcgcgtatgaggcgatgcgtaagctgcaccgccaaatcagcagcgaagtcagcaaaaaaggcatggcggacaacatcaaactcggcgcgggcggcatccgcgaagtcgaatttatcgcccagattttccagatgatacgcggcggacaaatgcgcgcgctgcaactgaaaggcacgcaggaaacgctgaagaagcttgccgagctgggcatcatgctgtctgaacacgtcgaaaccctgcttgccgcctaccgcttcctgcgcgatgttgaacaccgcctgcaatactgggatgaccagcaaacccaaaccctgccgacctcgcccgaacagcggcaactgctcgccgaaagcatgggtttcgacagttattccgctttttcagacggtctcaatgttcatcggaacaaagtcaatcagttgttcaacgaaattttgagcgaacccgaagagcaaacgcaagacaacagcgaatggcaatgggcatggcaggacaaacccgacgaagaagggcggcgatgccgtctgaaggcgcacgggttcgatgccgaaaccgtcgccgcaaggctcgaccaaatccgccacggccataaataccgccatctttccgcacacgcccagccgcgtttcgatgcggttgtgccgctgttcgtacaggcggcggcagcgcaaagcaacccgaccgatacattgatgcggctgttggattttctcgaaaacatcagccgccgatccgcctatctcgccttcctcaacgaacatccgcaaaccttggcgcaactggcgcagattatgggccaaagttcttgggtggcggcgtatctgaacaaatatccgattttgttggacgaactcatcagcgcgcagcttttggataccgcgtttgattggcaggcgctcgccgccgccctttcagacgacctcaaagcctgcggcggcgatactgaagcgcaaatggacaccctgcgccgcttccagcacgcccaagtcttccgtctcgccgtccaagacctcgccggactgtggacggtagaatccctct

附录Bccgaccaactctccgccctcgccgacaccatcctcgccgccgccctgctgtgcgcatgggcggacatgcccaaaaaacaccgcgacacaccgcaattcgccgtcgtcggctacggcaaactcggcggtaaagaactcggctacgcctccgacctcgacctcgtctatctctacgacgacccccaccccgacgcaggcgacgtgtacagccgcctcgcccgccgcctgaccaactggctttccgccgccactggcgcaggcagcctctacgaaaccgacctgcgcctgcgccctaatggcgacgccggtttcctcgcccacagcatcgccgcctttgaaaaataccagcgcgaaaacgcctggacgtgggaacaccaatcccttacccgcgcccgcttcatctgcggcacgtccgaaattcagacggccttcgaccgcatccgcaccgaaatcctcaccgccgaacgcgaccaaaccgccttggcaggcgaaatcatcgaaatgcgcgaaaaaatgttccccacccacccgcctgccgacagcaacgtcaaatacgcgcgcggtggcgtggtcgatgtcgaatttatcgtccaatatctgatacttgcccatgcccgccagtatccgcaactcttggacaactacggcaacatcgccctcttaaacatctccgccgactgcggtttgattgacaaaaccctcgccggacaaagccgcaccgcctatcgcttctaccgccggcagcagcacaacaccaaactgcgcgacgcggcaaaaaccgaagtaaccggcgaactgttggcacattacggcaatgtcaggaaattgtggcgggaagtgttcggcgaagaagcggcaaccgtctgaacaaaaaatgccgtctgaagcctgacaatctgggtttcagacggtattttcgtaccgtgccgttttaaggttgcggcagagctaaagcgatttatcgggaatggctgaaacccaaaaaccggattcctctttcgcgggaatgacgggatttcaggcttctgtttttgtgggaatgatgggattttttatccaagcaaaaatcaaaacaaacaaataagaaccgtttaaaaccccgccgtttccattaaaatagcgcattctactttttagacggccttggattcggatttcaagtgcaacactagtgtattagtggttggaacagattcaagaataaaacacttggcgtttcgtagccaagtgtttttcttggtcggtggttcaactcatcttgaaccctgcgtatctcccgatcactgatgttacggaaatcggtttgtttggggaagtattgccggatgagtccgttggtgttctcattcagccctttctcccaagaatggtaagggcgacaaaaataagtctccgctttcaatgctttggttattttggtgtgttggtagaactctttgccgttatccatggtaatggtgtgcaccctgtctttatgtgcctttaatgccctaacagctgcccgggcagtgtcttcggctttgaggctatccaatttgcagatgatggtgtagcgggtaacgcgttcgaccaaggtcaataatgcgcttttctgtcctttgccgacaatggtgtcggcttcccaatcgccgatacgggatttctggtcgacgatagcgggtcggttttctatgccgacacggttgggtactttgcctctggtccatgtgctgccgtagcgtttgcggtagggtttgctgcatattctgagatgttgccacaacgtgctgccgttgcttttgtcttggcgaaggtagcggtaaatggtgctgtggtggagcgtgatctggtggtgtttgcacaggtaggcgcatacttgttcgggactgagtttgcggcggataagggtgtcgatgtgctgaatcagctgcgaatcgagcttatagggttgtcgcttacgctgtttgatagtctggctttgccgctgggctttttcggcgctgtattgctgcccttgggtgcggtgccgtctgatttcgcggctgatggtgcttttgtggcggttcagctgtttggcgatttcggtgacggtgcagtggcgggacaggtattggatgtggtatcgttcgccttgggtcagttgcgtgtagctcatggcaatctttcttgcaggaaaggccgtatgctaccgcatactggcctttttctgttagggaaagttgcacttcaaatgcgaatccgccgacctctttcagttacagcagcttgatccctttcccttatccaacgggggaaggctaggatagggtggcttgcaaatatacagaacaagggacaagagccaccctctctccaaccctctccctccgtacgggagggggtggattctcgcgggcgaagcccacgctacggttagcctttaccccagcacaaacaattcccgcccgtgcgccttcagccaacttttagcattgtcggtatgcggcgtcagcgtgttcaccaaatgccaaaagcgcggactgtggtcggggtggcggaggtggcagagttcgtggatgcagacatagtcggcgacgtattcgggcgtgccgatcagccgccagttgaggcggatgccggtgtgcgggcggcatacgccccaaaaggttttggcgttgctcaggtctgtggcggtgggcgtcagtcctgtttcggctgcgtgtttttcaaggcggggcagcaggtattcgcgggcgcgttcgttcaacaggcggcgcaggtggtcgatttgtgcggcggtttcttttcggggaagcaggatttcagacgacgtgatacggatatggctttggctgtgggtatccagcttggtctttattccccgataccaaatccactcgggtaagtttgggtgggaaacaggatgcacgggcgttttggcaagcgtgttccgcaaaatcgtttcgtttgccgccagccagtttgctaacgcgtggtcttgaaaaaagggtgggacgttgatgctgaccgtctgcatattgacggggcgcagaatcagatttttcttggcactgcgtttgagttcgatttcgatgcacaaaccgtcggaaagagtataggtgaagcgtttcatagttgtgaataggtttcagaccggatacatcgtctgaaacaggaattttccatatcaggcggcaaacttcggataatatacaaaatcaaacatctgcgctacaaggttcagccgaacaagccgccgatatatttgctgatggtgatggcgctgagtactgccatcaaaccgaccacaatcacgccggaaacggtgagccacagcgggtgtttgtagtcgccgacaattttggttttgtaggcggcaatcagaatcagaccgagggaaatcggtaaaatcaggccgtttaatgcgcctacgaacaccagcacctgcgccggtttgccgatggtggaaaatacggcggtggacacggcgataaaggcaataatccatttgtttttattgcgttcgatagacgggctgagaccggagaagaacgacaccgaagtataagccgcaccaatcaccgaagtaatcgaagccgcccaaatcaccacgccgaaaatcagcaggccgatgtatcccgccgcatattcaaacggtgtggaagcagggttgtcgggattgagctgtacgccttggctgaccacgcccaaaaccgccaaaaacaatacaatccgcataatcgaggcaatcaggatcgcccgcaccgagctttggctcacttccggcaacgccgatttgcctttgatacctgcgtccagcagacggtgcgcaccggcgaaggtgatgtagccgccgaccgtgccgcccaccagtgtaacaatcgccattgcatcgagtttttccggcataaaggtatgcacggcggcatctgccagcggcggattcgcctgccatgccacataaaccgtcagcgcaatcattacgaaacccatcacttgggcgaatttgtccatcactttgcctgcttctttaaacagaaacacaccgatggcaatcacgccgctgatcacggcaccggtttccggtgacagtccggtcagcaggttcagacccaagcctgcgccgccgacgttgccaatattgaacgccaaaccgcccatcacaatcagcacagccaagaaatagcctgcgccgggcaagacctgattggcaatatcctgcgcctgtttttcggaaacggcgacaatccgccaaatattgagctgcgccccgatgtcgagcagaatcgagagcagaatcacaaagccgaaacttgccgccagtgcttgggtgaaggtggcggtttgggtcagaaagcccgggccgatggcggaagtcgccatcaggaatgcagcgccgattaaggcatttctgcggtttttttgatcagacataatcgcttatcctctataaaattggttgttgctgtgtttgggcgaaacctgcggttttagctacgcagaaactcgctttgctcgttttggcgaaacctgcggttttcagacggcctatgaactgtttttcaagcagaaactttgatgcctgccgccagtagttcctgccggattttttcggcaaacaccacggcgtgcggcccgtctccgtgcagacagatgctgtcggcttgcacggcaaccaggctgccgtccactgctttgacctgcccgtcccgcaccatctgcaatacttgggcgatggcttcttcgtcgctgtccacctgcgcatcggggcggctgcggggaaccagcgtaccgtcgggcatatagcggcggtcggcgaatacttcggaaatcacacccaagcctgcggcttttccggcttccaagagcaggctgccggaaagtgccatcaatttcaatttcgggtcgaaatccgccacaattcgggcaacggtatccgccagcgcacggtttttcgccgcttgattgtacattgcgccgtgcggtttgacataagccatttccaaaccctgatcacggcacaaggcctgcaatgcgcccaactggtaattcagacacgcccgcaaatcggcttcggacagattcatttcggtacggccgaagttttcccgatcgggatagccggggtgtgctccgatgcgcacgccgttttgttgggcatacgccaatgccgcccgaatatcggcaatgctgccggcgtgttgggcgcaggcgatgttggccgaagtaatcagctgcaacaaggcttcgtcgctgccgcagccttcggcgagatcggcgtttaaatcaacctgcttcatgggtgattctccgtatttggttcagataggcttgtttttgcgccgcagggcggtggcttctttcaagccgattattttgaatttgactttgctgccgaagcgcacctgtgccagcctgcccaaatcggcggcggcaacggtagcgattttcggataaccgccggtggtttgcgcatcggccagcaggataatcggtttgccgccgggcggcacctgcacggttcctgcctgaacagcgtgggacagcatttccaaaggttgcgacagggtcagcggctgtccgtcgaagcggtagcccatgcggttgctatcgctttgcagcgtccacgtttcccgttccagattcagacgccctttttcactgaaagcggcatattccgacgaaggaacaaggtggacggtatcggtaaacggtatcggggcaatgccgactttggacaattcctgcgcacctttgccgatggggagataatcgcctttttgcagcattctgccctgatggccgccgaaaccggctttcaggtcggtgcttctcgaacccatcacttccggcacatcaaatccgcccgccacgcacacatagccgtacatgccctgcacggcacgcaccagtttcaaggtctgccctttgcgggcggtataacgccaatacgaatagaccggttcgccgtccaattccgcctgatacacggcaccggtgagacaaaacggcgtatcccgttcaaacaccagcattatcccgcccaaagcgatttcgattgcggccgtgccttcgtcgttgcccaataaaatattgcccgccgccaaagcaaccgtgtccatcgcaccggcatgaccgatgccgtaacgccggtgtccgtagcgtccggtatcctgaatatgcgccggtgcctgcactgccgaaacgtgaatcatggctcaatcctttctgcaacaaagcggacttggtcacccgccgccagcagggtcggcggattcaaatcggctcggaacaagggtaattcggttctgccgataatctgccagccgccgggcgaagcgaacggatacacaccggtctgactgccgccgataccgaccgaaccggcaggaacggacgttctcggcacggcacggcggggcgtgtgcaatgcttcgggcaagccgcccagataagggaaaccgggctggaagcccatcataaatacggtataagtttgcgccgtatggcggcggacgatttcggaaataaccgtctgatggaaagcagcgacttccgccaaatccgggccgtattcgccgccgtagcagacgggaatttccaccagtttgccctgatggtctgtaacggcggtgtgttcccacacatattgcaattcatcggcaagcgtcgccaaatcggtatcgaaacgggtaaacacggt

附录Bcagattgttcatgccgaccaccacttcctcaatcctgtcgtgctgcccgagcgcagcggcaaacgcccacaacttttgctgtttgcccagttcggaaggcgcattcagtcggtagaccaaagcggattcgctgattggtgtgatctctattctcatttgttgttcattttggttatgttttaatgaatctatatgcaggggcggcggtttgtcaatatcttctgtgctgcatcatcaaaccgtcgattggaaaagtgctgccctgccgctgcactttttcagacgaccttaaaccgtttctattaaaatagcgcattccacttttcagacggcatccttatgtttcccgaccaatccgcccccaacctgctgcaaggcttgaatcccgaacaactctccgccgtaacctggccgccgcaatccgcacttgtgctggcgggcgcgggcagcggcaaaacgcgcgtgctgaccacgcgcatcgcatggctgttgcaaagcggacaagccagcgtgcacagcattatggcggtaacgtttaccaacaaagccgccaaagaaatgcaaacccgtttgggcgcgatgattcccatcaatgtccgcgccatgtggctcggcacgttccacggtctctgccaccgctttttgcgcctgcaccaccgcgacgccggtctgccgtcttcctttcaaatcctcgacggcggcgaccagctttccctcatcaaacgcctgctcaaaagcctcaacatcgccgaagaaatcatcgcgccgcgttcgctgcaaggctttatcaacgcgcaaaaagaatccggtttgcgcgcttccgtgttgagcgcgcccgatccgcacacacgccgcatgattgagtgctacgccgaatacgacaaaatctgccaacgcgaaggcgtggtcgattttgccgaactcatgctccgcagctacgaaatgctgcaaaacaacgaaatcctgcgccagcactaccaaaaccgcttcaaccacattctcgttgacgagttccaagacaccaacaaactgcaatatgcttggctgaaactgattgccggcaaccacgcagcagtatttgccgtcggcgacgacgaccaaagcatttaccgtttccgtggcgcaagcgtcggcaacatgaccgcgctgatggaagaattccacatcgacgcgcccgtcaaactcgaacaaaactaccgctccgtcggcaacatccttgccgccgccaatgccgtgattgaaaacaacgacgaacgactcggcaaaaacctgcgcaccgacgccgaagcaggcgacaaaatccgctactactccgcctttaccgacctcgaagaagcccggttcatcttggacgaaaccaaagccctcgaacgcgaaggctgggatttggacgaaatcgccgtcctctaccgtagcaacgcccaatcccgcgttatcgaacaaagcctgttccgcagcggcattccctacaaaatctacggcggcttgcgtttttacgaacgccaagaaatcaaacacgcgctcgcctacctgcgcctcgccgtcaatcccgacgacgacaacgccctcttgcgtgtcatcaacttcccaccgcgcggcatcggtgcacgtaccgtcgaaaatcttcagacggcctcaaacgaacaaggcatcaccctctggcaagccgcctgcaacgccggcgcgaaagccgccaaagtcgtcgccttcgtccgcctgattgaagccctgcgcaaccaagtcggacaactgtccctgtccgaaatcatcgtcggcatcctcaaagacagtggcttgaccgaacactaccgcacccaaaaaggcgacaaccaagaccgtctcgacaaccttgacgaactcgtcaacgccgccatcgaattcaaacccgaagacagcaacttcgaaatcctgcctgaaaacatttcagacgaccccgccttccccattctcgccttcctaagcaatgccgccctcgaatccggtgaaaaccaggcaggcgcaggcgaaaaggccgtccaactcatgaccgtccacgccgccaaaggcttggaatttaacgccgtcttcctcaccggcatggaagaaggccgcttccccagcgaaatgagccttgccgaacgcggcggcctcgaagaagaacgccgcctcatgtacgtcgccatcacccgcgcccgcaaacgcctctacatcaccatggcgcaacaacgcatgctgcacggacaaacccaattcggcatcgtctcccgcttcgtcgaagagatcccacccgaagtattgcactacctgtccgtcaaaaagcctgcctacgacagttacggcaacacgcgccaaaccgccgcatccaaagataaaatcatcgacgactacaaacagccccaaacctacgcaggtttccgtatcggacaaaacgtccgccacgccaaattcggcaccggcgtgattatcgatgccgcagataaaggcgaatccgcccgactgaccatcaatttcggcaaacagggcgtgaaagagttggacaccaagtttgcgaaattggaagagatgtaaatttgaaatgtaggtcggatattcgtatccgacctacggcaaaaaccttagcaggagagaatagaaacccgtagcgtgggctttttctatgaatcaagcccaaaatttcagacggcatttttagccgtcattatcgtggatgaagcccacgctacaatgtacacacagagcaaatagagatgtgggtcggatattcgtatccgacaaaaacatttgacgcgtctattgtttccgaaacaccgctgttggaaatgtcggatacaagaatctgacttacggcaaaaaacgtagtaaggacaaagcaaaaggccgtctgaaaacgggaagggcaattttgccgcaaccgccgccgtcattcccgcgcaggcgggaatccagacctttcggcacggaaacttatcggataaaaggtttctttagattccacgtcctagattcccgccggaacataaatgacggacggtaaaagccgggtatgaatacccaccctctgttatcactgagatcaataaggaagaacattatgtcccaagtttttaaagattttgacttgtcctccgtatggaaaactaatagttgggcagatgaaaactacaaagaagccccgtttacccctgaaattttggctgccgtagaaagtgaactgggctataaattgccgcaaagttttattgaattgatggcagtacaaaacggcggaatatttgtcaaaaactgttttccgaccacgcagagaaattcgtgggcggaaaatcatgtgcaaatttgcgaggtatcgggaatcggttttgaaaaagaagggagtttgtgcggcgcgatggggcaaaaactttggctggaagaatgggaatacccgcctatcggcgtgtattttgccaacgacccgtcaggcggtcatgccatgtttgccttagactatcgggcgtgcggcaaagacggcgagccgaaagtggtgtttgtcgaacaagaatcggattttgaaatcgtcgaacttgcccccgattttgaaacctttatccgcagcttgcggcatgaagatgagtttattgacgaagaaatataaaacggtggttgaaaaactgaaatcatcaagagaaaacgggcgaaataacgggtaatcgcttgaatccgtaaggaaaacggtttggtggaacgcgccatccaagacctttgcaaaaaactgtccccgacagcattgacattattaacagaacttatcaattttggagctatctcaaatataattcggttatcctgttgtatccattaaatcatatgcttcaattaattgttgttctagctcttataccaattttggattgcgaattcctgacacaatctcaaattcttctgcatctatgcaaacacctgcataaatttcaataacaagggaacgcaataattgaagctcttctcttgttaaagaaataataatgtcatcacctttgtaattgattatattcataataattttatttttgtttgtcaaagtaagttttgcctaaggttggtctaaatgcagttccaccatcttttgaatttgggtctctgattacaattgctccagacttatcatcccaaattgctcttatgtgtttggattgtaatcttcgaattcccaagaaaaaaatcgtaataagtttgaaagtgtcaaatcccaagtttcttttgagcaatattctaatattttatcaatttcacttttaataatcttatgatcaaactgttctaatattaatgcattagaccaaaaaaaaccttctttattacaatgatgggaaatccatttaggagaacaaatgcaaagtgaaaaaatagatgagccttgttctccttcgattccgatatccaaatctatccatctatggaaattatctggaatttcgggggtaaatttttcaaaatcaatatcatataaatttatgctttttaaatccaatttaatcattagggctgtcctagataaatagggaaattcaaattaagttagaattatccctatgagaaaaagtcgtctaagccggtataaacaaaataaactcattgagctatttgtcgcaggtgtaactgcaagaacagcaacagagcccgacagcattgtttatacggattgttatcgtagctattcatttacgcaagtttaacggcattcccaaagcgcattttgagctgtatttaaaggagtgcgaatggcgttttaacaacagtgagataaaagttcaaatttccattttaaaacaattagtaaaatcgagtttatcttagttgtccaggacagccccattatttttataacaccgtgaagccgcacagcagtttgaacagtgatacgccgtttgcgggcttacgagtttattttcccggcctgcagtttgagcaatacggtgatttcctacggttaatacaaatgtttacacattgatacatttcatttatagttccgcctatttgaaaatagaaaatatgaattcgaccgcaagtaaaaccctgaaaggattgtcgctggtgtttttcgcctctggattctgcgccctgatttaccaggtcagctggcagaggcttctattcagtcacataggtatcgatttgagttcgattactgtcattatttctgtatttatggtcggcttgggtgtaggtgcgtatttcggtggacgcattgctgaccgttttccttcaagtatcatccccctgttttgcatcgctgaagtatccatcggtctgttcggtttggtaagcaggggtctgatttccggcttggggcatcttttagttgaggctgatttgcccatcatcgctgctgccaatttcctcttattgctgcttcctacctttatgatgggcgcgaccttgcccttgctgacctgtttttttaaccggaaaatacataatgttggcgagtctatcggtaccttatattttttcaacactttgggtgcggcactcggatcgcttgccgccgccgaatttttctacgtcttttttaccctctcccaaaccattgcgctgacagcctgctttaaccttctgattgctgcttcagtatggctgcgttacagaaaggatggatatagtgaacactaaaccgaatactagtttgatttatatgctttctttccttagcggcttattgagcttgggtatagaagtcttgtgggtgaggatgttttcgttcgcagcacagtccgtgcctcaggcattttcatttacccttgcctgttttctgaccggtatcgccgtcggcgcgtattttggcaaacggatttgccgcagccgctttgttgatattccctttatcgggcagtgcttcttgtgggcgggtattgccgactttttgattttgggtgctgcgtggttgttgacgggtttttccggcttcgtccaccacgccggtatcttcattaccctgtctgccgtcgtcagagggttgattttcccgctcgtacaccatgtgggtacggatggcaacaaatccggacgacaggtttccaatgtttatttcgccaacgttgccggcagtgcattgggtccggtccttatcggctttgtgatacttgatttcttgtccacccaacagatttacctgctcatctgtttgatttctgctgctgtccctttgttttgtacactgttccaaaaaagtctccgactgaatgcagtgtcggtagcagtttccctaatgttcggcatcctcatgttcctactgccggattctgtctttcaaaatattgctgaccgtccggataggctgattgaaaacaaacacggcattgttgcggtttaccatagagatggtgataaggttgtttatggggcgaatgtatacgacggcgcatacaataccgatgtattcaatagtgtcaacggcatcgaacgtgcctatctgctaccctccctgaagtctggcatacgccgcattttcgtcgttggactgagtacaggttcgtgggcgcgcgtcttgtctgccattccggaaatgcagtcgatgatcgttgcggaaatcaatccggcataccgtagccttatcgcggacgagccgcaaatcgccccgcttttgcaggacaaacgtgttgaaattgtattggatgacggtaggaaatggctgcgtcgcca

附录Btcctgatgaaaaattcgacctgattttgatgaatacgacttggtactggcgtgcctattccaccaacctgttgagtgcggaatttttaaaacaggtgcaaagccaccttaccccggatggtattgtaatgtttaataccacgcacagcccgcatgcttttgctaccgccgtacacagtattccctatgcataccgctatgggcatatggtagtcggctcggcaaccccggtagttttccctaataaagaactgctcaagcaacgtctctcccggttgatttggccggaaagcggcaggcacgtatttgacagcagcaccgtggatgctgcagcacaaaaggttgtctctcgtatgctgattcagatgacggaaccttcggctggggcggaagttattaccgacgataatatgattgtagaatacaaatacggcagagggatttaaccgtcttaaagggtttcaggcaacgcaggttttaggtaacgtcctgctagttcaaaaaaaccgcatcacagcagtcgggacaaaatggtttaaacattttgtcccgaattcttattcctatatatagtggattaacaaaaatcaggacaaggcgacgaagccgcagacagtacaaatagtacggaaccgattcacttggtgcttgagcaccttagagaatcgttctctttgagctaaggcgaggcaacgccgtactggtttttgttaatccactataccacgaattacggtgtaaaaatttatatgaccttataaaatcaaataagaatcgttatcataacatgattgtatttattgggtttttttgggcgttttgccgatatttaccttttaatggtttttgaaattcgctaaaatacgaaattattgtagaaattttgttaacggatttgggtgtaaccatgttgtccgcttactttcccgtctttgtctttatcctcatcggcctcgcggccggcgtgctgtttatcctgctcggcacgattttaggcccgaaacgccactatgccgaaaaagacgcgccttacgaatgcggttttgaagcttttgaaaacgccaggatgaagttcgacgtgcgctattacctcgtcgccatcctcttcatcctgtttgatttggaggtcgcgtttatgctgccgtgggcagtcgtgttcaaagatttgggcgcgtacggcttctggtctatgctggtgtttatcgttgttctgacggtaggctttgtttacgaatggaaaaaaggtgcgctggaatgggaatagaaggcgttttgaaaaaaggtttcatcaccaccagcgcggatacggtgctgaactatatgcgtaccggttcgttgtggccggttactttcggcttggcctgctgcgccgtggaaatgatgcacgcgggtatggcgcgttacgaccttgaccgtttcggtattattttccgtccgtccccccgtcaggccgacctgatgattgtggcgggtacgctcaccaataaaatggcgcccgccctgcgccgagtgtacgaccagctcgccgagccgcgctgggtattgtctatgggctcatgtgccaacggcggcggctattatcactattcttattccgttgtgcgcggtgccgaccgcgtcgtgccggtagatgtttatgtgccgggttgtccgccgactgcggaagccctgatttacggcctgattcagctccaacaaaaaatcaagcgcacttccaccattgcgcgtgacgagtaaggagaggacgatatggcaagcattcaagacttatacgaaaccgtcagccgcgttttgggcaatcaggcaggcaaagtcatttccgctttgggcgagattaccgtcgagtgtctgcccgagcactatatttcagtcatgaccgcattgcgtgaccatgaagagttgcatttcgagcttctggttgacttgtgcggtgtcgattacagcacttacaaaaacgaagcatggcagggcaaacgctttgccgtcgtcagtcagttgctttccgttaaaaacaatcaacgcatccgcgtgcgcgtctgggtttcagacgacgacttccccgtagtcgaatctgtagtcgatatttacaacagcgcggattggtacgaacgcgaagccttcgatatgtacggcatcatgttcaacaaccatccggacttgcgccgcatcctgaccgattacggcttcgtcggacatccgttccgcaaagacttcccgatttccggctatgtggaaatgcgttacgacgaagagcaaaaacgcgtgatttaccaacctgttaccattgagccgcgcgagatcacgccgcgtatcgtccgtgaggagaactacggtggccaataaattaagaaactacaccatcaacttcggcccgcaacaccctgcggcgcacggcgtattgcgtatgattttggagctggacggcgaacaaatcgtccgtgccgacccgcatatcggcctcttgcaccgaggtaccgaaaaactggcggaaaccaaaacctatctgcaagccctgccctatatggaccgcttggactatgtttccatgatggtcaatgagcaggcgtattgtttggcagtagaaaaacttgtcggtatcgatgtgcccatccgcgcccaatacatccgcgtgatgtttgccgaagtaacgcgcatcctcaatcacttgatgggcatcggttcgcatgccttcgacatcggcgcgatgaccgccattctttacgccttccgcgaccgcgaagagctgatggacttgtacgaagccgtgtccggcgcgcgtatgcacgccgcctacttccgtcccggcggcgtttaccgcgacctgcccgactttatgcccaaatacgagggcagcaaattccgcaatgccaaagtattgaagcagctcaacgaatcccgcgaaggcaccatgctcgactttatcgatgccttctgcgaacgcttccccaaaaatatcgacacactcgaaaccctcctgaccgacaaccgtatttggaaacagcgtaccgtcggcatcggcgtcgtctcccccgaacgtgccatgcaaaaaggctttaccggcgtgatgttgcgcggttcgggcgtggaatgggacgtgcgtaagacacagccttacgaagtgtacgacaaaatggatttcgacatccctgtcggcgtgaacggcgactgctacgaccgctacctctgccgtatggaagaaatgcgtcaatccgtacgcatcatcaaacaatgttccgagtggttgcgtgtcaatccgggtccggtcattaccacaaaccacaaattcgctccgcccaaacgtaccgaaatgaaaacaggtatggaagacctgattcaccatttcaaactctttaccgagggtatgcacgttcccgagggcgagacctacaccgctgtcgaacatccgaaaggcgagttcggcgtttacatcatttcagacggcgcaaacaaaccctaccgcctgaaaatccgcgcacccggcttcgcccatctgcaaggcatggacgaaatggcaaaaggccacatgctcgccgacgtcgttgccatcatcggtacgcaggacatcgtattcggggaggttgaccgataatgttatccgcagaatctttaaaacaaatcgacatcgagttggcaaaatatcctgccgaccaacgccgctccgcgattatgggcgcattgcgtattgcccaaaccgaaaaaggctggcttgctcccgagaccatcgcttttgtcgccgactacatcggcatcacgcctgcacaagcctacgaagtcgccactttctacaatatgtacgaccttgagcctgtcggcaaatacaaactgaccgtttgtaccaacctgccctgcgccctgcgcggcggtatggctaccggcgaatacctcaaacaaaaactcggtatcggctacggcgaaactacccctgacggcaagtttacccttgtcgaaggcgaatgcatgggcgcatgcggcgacgctcccgttatgctggtcaacaaccacagcatgtgcagctttatgaccgaagaagcgattgagaagaaactggcggagttggagtaggtcgtctgaaacgacgatttaaacgtaggtcggatacttgtagccgacagagtgggtaaaaaggcaaaatgtcggatttaagaatccgccctactgaaataccgaaatgccgtcattcccgcgcaggcgggaatccaccggtaagattcggtttctgaatttaataagacattgcttaccattgaggatggattcccgcctgcgcgggaatgacgacagacaagcaagtggtcgagatccaacaaaaacgattaaaggtcgtctgaaaatatcgatttgataaactagattttatttcagacgacgttacaagccggtacacaaagacatcttaaggtcgtctgaaacagcggccgcaaccgatacgaaaacaaacaggcacaccaaaaatggctatttaccaatcaggcgtgatttttgaccaagtggataccgccaatcccgattgctggacattggacgaatacgtcaaacgcggcggctataccgccctgcgtaaaattctgtccgaaaacatctcgcaaaccgatgtgattgacgaagtcaaaacctccggtttgcgcgggcgcggcggtgcgggcttcccgaccggtttgaaatggagctttatgccccgttctttcccgggcgaaaaatatgtggtttgcaacaccgacgaaggcgaaccaggtacgtttaaagaccgcgacatcatcatgttcaatccgcatgccctgatcgaaggcatgattatcgccggttacgcgatgggcgcgaaagccggttacaactatatccacggcgaaatttttgaaggctaccaacgctttgaggccgctttggagcaggcgcgtgccgcaggctttttgggtaaaaatattttgggttcggattttgaatttgaactcttcgcccaccacggctacggcgcatatatttgcggcgaggaaaccgcattgctcgaatcgctggaaggcaaaaaaggccagccgcgctttaagccgccattccctgcttcgttcggcctgtacggcaaaccgactaccatcaacaatactgaaacgttctcctccgttccattcattatccgtgacggtggacaggcatttgccgataaaggtattccgaatgcaggcggtaccaaattattctgtatttccggccatgtcgagcgtccgggcaactatgaagtgccattgggtacgccgtttgccgaagtcttgaaaatggcgggcggtatgcgcggcggtaaaaaactcaaagccgtcattcccggcggttcgtccgcgcccgtattgcctgccgacatcatgatgcagaccaatatggactacgactcgatctccaaagcaggctccatgctcggttccggcgcgattatcgtcatggacgaagacgtgtgcatggtcaaagcccttgagcgtttgagctacttctactacgacgagtcttgcggccaatgtaccccctgccgagaaggtacgggctggctttaccgcatcgtccaccgcatcgtagaaggcaaaggtaaaatggaagatttggatttgctggattccgtcggcaaccaaatggcaggccgcaccatctgcgccctcgccgatgctgccgtcttccccgtccgcagctttaccaagcatttccgtgatgagtttgtgcattacatcgaacacggcgggccgatgaaagagcataagtggggagggtggtaatggtggaagctaaaatttttattctatacggtgcagccaacaaaggtaagagtacgacactcaatacgctttttaatcagatttgtcggaaattttctaaatttctagtcttttttgaaagacatggaaacggcttagattttgttgcagtatttgatcatgaaggtcagagaattggtttttattcatctggtgataatgaatacgaggttaggggaaatttatacaaactttattcgcataattgtgattttatttttggcacgtcaaggacacggggtggtagttgcgatgcagtaggatgttatgcagagttattgcatggcgatgtaaatataattaattggtgtgaaaagtttgagcctacagatgaagacaatgagcgtgctgttaaagagttatttaagtcatttaaaaatataataaatgagttatagttttagttggttttatattggttaaaagcaaaatgctaaaaatttaactttgccgtcattcccgcgtaggcgggaatccatagtggaatttacagaacccgatatttgaaaagcagttgccgaaattcaaaaaatggattcccgcctacgcgggaatgacggcgggagtaggcagatgttttcagatgaaaacggttgtaaatgatattaaaaaagttgttgtttatattgcaggaaaaatgaatacgaaaccatccgcttactagacaacctgccgtatatattttggcaaacggtaaaaatggaacactctatatcggtgttaccatgaatttgccggaaagggtttggcagcacaaaaaccatgtcaatattgatggctttactgcccgatatgatgtgcatgatttagtttggtatcagttttttgagaatatgcctgaagcagttgccaaagaaaaaacgatgaaa

附录Baaatggcgacgtgaatggaagattaaactgattgaagaacaaaatactgaatgattggacttgtcgggcgtgttgtttgtttagttttatttctggaactttaaaaactgtcgttattccagccccacctacgcgcagacaggctacggcgggaatcaccgcaaaagttaagaaaccaatgtttgaaaacagttaccgaaaacccaagaatggattcacgcctgtgcgggaatgacggcaaggtggcagtaaacgttttaaacagtattgattgtcaatgaaactcaaaaggccgtctgaaacccatttttcagacgacctccataaaagattatttatcaaatacccgtaactaggaacgaaccatgttacaaatcgaaatcgacggcaaacaagtatctgtggagcagggcgcgacggtgattgaagccgcgcacaagctcggtacttatattccgcatttctgttaccacaaaaaactttccatcgccgccaactgccgtatgtgtctggtgaacgtagaaaaagccccaaaacccctgcctgcctgtgccacgccggttacagacggcatgattgtgcgtacgcattcggcaaaagcccgagaggcgcaggaaggcgtgatggagttcctgctcatcaaccatccgcttgattgtccgacctgcgaccaaggcggcgaatgccagttgcaggatttggcggtgggctacggcaaaaccaccagccgctacaccgaagaaaaacgttccgtcgtcggcaaagatatggggtccttggtttccgccgaggaaatgagccgctgtatccactgcacccgctgcgttcgtttcactgaagaaatcgccggtttgcaggaaattgcgatggtgaatcgcggcgaacactccgaaatcatgccctttatcggcaaaacggtggaaaccgaattgtcgggcaacgtcattgatttgtgtcccgtcggcgcgctgaccagcaaaccgttccgcttcaacgcgcgtacttgggaattgaaccgccgcaaatccgtttccgcccacgatgctttgggcagcaacctgattgtgcagaccaaagaccataccgtccgccgcgtgttgccgttggaaaacgaagcgattaacgaatgctggctgtctgaccgcgaccgtttcgcctacgaaggcctgtatcacgaaagccgtctgaaaaacccgaaaatcaaacagggcggcgagtggatggacgtggattggaaaaccgcgttggaatatgtccgcagcgcgattgaatgtatcgccaaagacggcaagcaaaaccaagtcggcgtttgggcgaacccgatgaatacggttgaagaactgtatctggcgaagaaactcgccgacggcttgggtgttaaaaactttgcaacccgtttgcgccaacaagacaaacgtctttcagacggccttaaaggtgcgcaatggttgggacaaagcattgaatctttggctgacaacgatgccgtattggtagtcggtgcgaacttgcgcaaagaacagccgctcctgactgcccgcctgcgccgcgccgccaaagaccgtatggcattgagcgtattggccagcagtaaagaagaattgtttatgccgcttctgtctcaagaagccgcacatcccgacgagtgggcaggccgtctgaaaaacctgtctgtcaatgcggaacacgccgttaccgccagcctgaaaaatgctgaaaaagcagcggtgattttgggcgcggaagtgcaaaaccatcctgattacgccgcggtttacgccgccgcgcaagagctggctgacgcgaccggcgcagtgctgggcattttgccgcaagccgccaacagcgttggtgcggatgtcttgaatgtaaactccggcaagagcgttgtcgaaatggtaaacgcgccgaaacaggcagtcttgctgctcaacgttgagcctgaaatcgatacggcggacggtgcaaaagccgtagccgcgttgaaacaggcaaaaagcgtgatggcgtttacgccgtttgtcagcgaaacgctgctggacgtgtgcgacgtgttgttgccgattgcaccgtttaccgaaacctcaggcagcttcatcaatatggaaggccgtctgcaatccttccacggcgtggtacaaggcttcggcgattcgcgtccgctgtggaaagtgttgcgcgtattgggcaacctgtttgacctgaaaggttttgaataccacgataccgctgcgattttgaaagacgcgctggatgtggaaagcctgccgtccaaactggacaaccgcaacgcatggacaggggagggcgttcagacgacctcagaccgcctcgtccgtgtcggcggcgtcggtatttatcacaccgattctatcgtgcgccgttccgcaccgttgcaagaaaccagccatgccgccgtgcctgctgcgcgtgtaaatccaaatacattggcacgcttgggcctgcaagacggacaaaccgctgtcgccaaacaaaacggcgcaagcgtatcggttgccgtcaaagccgatgccggactgcctgaaaacgtggtgcatctgccgctgcataccgaaaatgccgcgctgggtgcgttgatggacactattgaactggcgggagcttgattatgcaggaatggttccaaaacctctttgccgcaacgctcggtctgggcgatttgggtattactgtaggcttggtggtatccgtcatcgtcaaaattgtgattatcctgattccgctgattctgaccgtcgcctacctgacttatttcgaacgtaaagtcatcggcttcatgcagcttcgcgtcggtccgaacgtaaccggcccgtggggtctgattcagccgtttgccgacgtgttcaaactcttgtttaaagaagtaacccgtccgaagctgtcaaacaaagccctgttctatatcggcccgattatgtcgcttgccccgtctttcgcggcgtgggcagtgattccgttcaatgaagaatgggtgctgaccaacatcaatatcggtcttttgtacatcctgatgattacctcgctgtcggtttacggcgtgatcatcgcgggctgggcttccaactccaaatattcgttcttgggcgcaatgcgtgcttccgcgcaaagcatttcctacgaaatcgccatgagtgccgcgctggtgtgcgtcgtgatggtgtcgggcagcatgaacttctccgacatcgttgccgcgcaggcaaaaggcatcgcaggcggttcggtattctcttggaactggctgccgctcttccccatcttcatcgtctatctgatttccgccgttgccgaaaccaaccgcgcaccgtttgacgtggcagagggcgagtctgaaatcgttgccggtcaccacgtcgaatattccggcttcgcattcgcgctgttcttccttgccgaatacattttcatgattctgattgccgcgctgacatcgttgatgttcctcggcggctggctgtctcccttcccgcaaagctggggcattgtcggtacgccttccgcattttggatgttcgcgaaaatggcggcggttctgtactggtatctgtggatacgcgccaccttcccacgctaccgttacgaccaaatcatgcgcttgggctggaaagtgctgattccgatcggcttcgcctacatcgtgattttgggcgtgtggatgatttcaccgctgaatttgtggaaataagtttcagacggcatcttgaggccgtctgaacaaagcgattttgaatacctaacgaaatccctgttttgagggaacataatatggctaacttagtaaaaacctttctgcttggcgaattggtaaaaggtatgggcgtaacgctcaaaaactttttcgcccgcaaagacacaatttatttccccgaagagaaaacgccgcaatccgtgcgtttccgcggtctgcacgcgcagcggcggtatccgaacggcgaagagcggtgtatcgcgtgtaagttgtgtgaggcagtgtgtccggcaatggcgattaacatcgaatcggaagaacgtgaagacggtacgcgccgcaccaagcgttacgacatcgacctgaccaagtgcatcttctgcggtttctgcgaagaggcatgcccgactgatgcgattgtggaaacccatatttttgaataccacggcgagaaaaaaggcgacttgcacatgaccaagccgattcttttggccattggcgacaaatacgaagctgaaatcgccaaacgcaaagccgctgacgcgccgtatcgttaatgctttggggcttcttggaaggttttaaatatggaaggactgattaatgcattgaaatatttagccgaacatgagccaatagataattttgaagaaattagaactagaaatagtccgattgagttgccaagtggattaagtaattttgaacaaaatatttttttaaaagaaaatttatccccaaaattacaaaatgatgatagcttgaagacgcattattggattatccgtgaatggggtgggattaaaagttttaaacaatctgctgaaaatagccagcttattcgtcaatttttatcggaacttaattcgggaaaattgagtagtggtttgttgaaaatttcatcattatctaaattggcttcttttatagattgtgagcgattcgccatttatgattcacgcgctattttttcgttgaattggttgttgtttaaatttacaaatgcagatttgttttttcagccacaaggtagaaatagggaactagaaatccgaaatatgaacgtattgtttcatttttctgatatcaaaccgaattatcggaaaccagacgtttcgtttcatcaatattgtgggttgttacaagatttggcgaaacaagtttatggtaaacaagcaaaaccgtatcacatagaaatgttgttattcaaaattgcgacaacgtggatttgtgcggatatggatcaactgattaagtttgattgtttgcgtaaccaggattttcagactgcttgaaaccatatttttgattaataaagaaagcatagactatgactttccaactgattttattttatatttttgcagtgataattctttatggcgcgctcaaaaccgtcaccgctaaaaaccctgttcacgccgctttgcatctggtgctgaccttctgcgtgagcgcgatgctttggatgctgatgcaggctgagtttttgggcgtgacgctggtggtggtttacgtcggcgccgtgatggtgttgttcctgttcgtcgtgatgatgttgaacatcgacattgaagaaatgcgtgccggtttctggcggcacgcgcctgttgccggtgtggtcggcacattgttggcggttgcgctgatcctgattctggtcaacccgaaaaccgaccttgccgcatttggtctgatgaaagacattcctgccgattacaacaatatccgcgatttgggcagccgtatttataccgactatctgttgccgtttgaattggcggcggtattgctgttgttgggtatggtggcggcgattgcgctggttcaccgtaaaacggttaatccgaaacgcatggatcctgccgaccaagtcaaagtacgcgccgaccagggccgtatgcgtctggtgaaaatggaagcggtcaaaccgcaagtcgaatctgccgaagaaagcgaagtttcagacgacctcaagccgaaagaggagggcaaagcatgattaccttgacgcattatttggtattgggtgcgctcctgttcggtatcagcgcaatgggtatctttatgaaccgcaaaaacgtgctggtattgctgatgtcgatcgagctgatgcttttggcggtgaacttcaactttatcgccttctcgcaacatttgggcgatactgccggacaaattttcgtattcttcgtattgaccgttgccgctgccgaatctgccatcggtttggcgattatggtgctggtgtaccgcaaccgacaaacaatcaacgttgccgatttggacgagttgaaagggtaaaggtaggttgggtcgagacctgacaagacaccgatgccgtctgaaaacccgataggaaaaacgatgaaatccatagacgaacaaagcctgcataatgcccgccgcctgtttgaaagcggcgacatcgaccgtatcgaagtcggtaccaccgcgggcctgcaacagattcaccgttacctgttcggcggcttatatgattttgcgggtcaaatcagggaagacaacatttccaaaggcggttttcgttttgccaacgccatgtatttaaaagaggctttggttaaaatcgagcagatgcccgagcggacttttgaagaaatcatcgccaaatatgttgaaatgaacattgcccatccgtttttggagggtaatggcagaagtacccgcatctggctggatttggtgctgaaaaaaaacctgaaaaaagtcgtgaactggcaaaatgtaagtaaaaccctgtatttgcaggcgatggaacgcagccccgtcaacgatttagaactgcgctttctgttaaaggacaacctgactgacgatgtggacaaccgtgaaatcatctttaaaggtatcgagcagtcgtattattacgaagggtatgaaaaaggctgagggtcgtctgaaaagcgatttcagactgtttcagacga

附录Bcctgattcggtaggtgatcagacgggagcggatgagaaaagaaattctgggtaagaataatccggtctgaaatattggaagaagaatgatggataaaaatcagttagaacaagaatttcataaagccatgttaaatatttatcaggaggctttgaatttgccgcaaccttacaaggcgacacgatttttacaaattgtaaatgaatttggtggtaaagaggcggcggataaattattgagtacgggggaaaagaagactcagaccggttttacagagctgattttgagtggtggcggagtccacgccttgaaatacagtatggaatatctggtgttacaaaagccgtggtgtgatttatttactgaagagcaattagctgtggcacgcaaacgattggagcgtgttggatttgtttttccgaagtaattttgtacgaaacaaacatagatttttaaatcaatcggattcaatcaaatgaacgatatgactttatatttgataattgcccttgttccgttggcaggctcgctgattgcgggtttgttcggcaacaaaatcggacgtgccggtgcgcatacggttacgatactcggcgtggcggtgtccgccgtgctgtcggcttatgtgctgtggggctttattgacggcagccgcgccaagtttgacgagaatgtctatacctggctgacaatgggcggcttggatttctccgtcggcttcttggtcgatacgatgacggcgatgatgatggtcgtggtaacgggcgtgtcgttgatggtgcatatctataccatcggctatatgcacgatgaaaaagtcggctaccaacgcttcttcagctatatttctttgtttacattcagtatgttgatgctgattatgagcaacaacttcattcagctcttcttcggttgggaagcggtgggcttggtgtcgtatctcttgatcggtttctatttcaaacgcccgagcgcgacatttgccaacctgaaagcctttttgatcaaccgtgtcggcgacttcggctttttgctcggtatcggcttggtgcttgcctatttcggcggcagcttgcgctatcaagatgtattcgcttatctgcccaacgtgcaaaatgccactatccaactgttccccggtgtggaatggtctttgattactgtaacctgtttgctcctgtttgtcggtgcgatgggtaaatcggcacaattcccgctgcacgtctggctgcctgattcgatggaaggcccgaccccgatttctgcattgattcacgccgcaaccatggttaccgccggtttgtttatggtgtcgcgtatgtcgccgatttatgaaatgagcagcaccgcgctgtcggtcattatggtgatcggcgcgattaccgccctgtttatgggctttttgggcgtgattcaaaacgacatcaaacgtgtagttgcgtattccaccctgtcgcaattgggctacatgaccgtggctctgggcgcgtctgcctattccgtggcgatgttccatgtgatgacccacgccttctttaaagccctgttgttcttggcggcaggcagcgcgattatcggtatgcaccacgaccaagacatgcgccatatgggcaatctgaaaaaatatatgccggttacttggctgaccatgctgatcggtaacttgtcgctgattggtacgccgttcttctccggcttctactccaaagattcgattatcgaagcggcgaaatacagcacactgccgggcagcggctttgcctattttgccgtcctcgccagcgtgtttgttaccgcgttttacgcgttccgccaatactttatggtgttccacggcgaagagaaatggcgcagcctgcccgaacaccattcagacggccacggcgaagaacatcacggtttgggtaaaaacgacaatccgcacgaaagcccgttggtggttaccctgcctttgattttgcttgccgttccgtccgtcatcatcggctacatcgccatcgaacccatgctctacggcgatttcttcaaagacgtgattttcgtcaacgccgacgcgcatccgactatacacatcatgaaggaagagttccacggcgcattggcaatggtgtcccacagcctgcattcgcccgtactctaccttgctatcgcaggcgtgttgagcgcatggcttttgtacgtcaaactgccgcacctgccagcgaaaattgcacagacgttccgtccgatttacgttttgtttgaaaacaaatactacctcgacgccctgtatttcaacgttttcgccaaaggcacacgcgcattgggcactttcttctggaaagtcggcgataccgccattattgacaacggtattgtcaacggctctgccaaactggtcggcgcgattgccgcgcaagtgcgtaaagcccaaaccggctttatctacacctacgccgccgctatggtgttcggcgtattggtcttgctcggcatgaccttctggggattgttccgataagaataaggtttcagacggccttaaaccttcaggccgtctgaaacgaagaaatatccacataaacacatttttattttaaccacaggttaaccactatgttttccaactacctactcagcttggcaatatggatacccatcgccgcaggcgtgctggttttggcaacggggtcggacagccgtgcgccgtttgcccgcgtgctcgccttcatgggtgcgcttgccggtttcttggtaacactgcccctgtttaccggtttcgaccgtttgagcggcggctatcaatttaccgagttccacgagtggattccgcttctgaaaatcaactacgcattgggcgtggacggtatttcagtgctctttatcatcttgaatgcgtttattacgctgttggtggtattggcaggttgggaagtcattcagaaacgtccggcgcagtatatggcggcattcctgatcatgtcgggtttgattaacggcgcgtttgccgcgcaggatgcgattctgttttatgtgttcttcgagggtatgctgattccgctgtacctgattatcggtgtatggggcggtccgcgccgcgtctatgcgtcggtcaagctcttcctctacacgctgatgggttcgctcctgatgctggttgcgatggtttacctttattatcaaacaggcagcttctctattgtcgatttccaaaacatcgaacagattccgttgggcgtacaacagcttttgtttgtggcgttcttcctgtcatttgccgtaaaagtgccgatgttccctgtgcacacttggttgccggatgcccacgttgaagcgccgaccggcggttcgatggtgttggcggccattacgctgaaactgggtgcgtatggtttcttgcgctttatcctgccgattatgccggatgcggcacgctattttgcccccgtgatcatcgtattaagtctgattgccgtgatttatatcggtatggtggctttggtgcaaaccgatatgaaaaaactggtggcgtattcgtccatcagccatatgggttttgtaacgcttgggatgtttttgtttgttgacgggcagttggacgactgggcattgaaaggtgcaatcattcaaatgatttcgcacggtttcgtgtctgccgcgatgtttatgtgtatcggcgtgatgtacgaccgcctgcacacgcgcaatattgctgattatggcggcgtggtcaatgtgatgcccaagtttgcggcgtttatgatgctgttcggtatggcgaacgcgggtttgcctgcgacttccggcttcgtgggcgagtttatggtgattatgggcgcggtcaaagtgaatttctgggtcggcgcgttggccgccatgaccctgatttacggtgcatcttataccctgtggatgtacaaacgcgttatttttggtgcgatccacaatccgcacgttgccgaaatgcaagacatcaattgccgcgaatttgcgattttggcaattttggcggtggctgttttgggtatgggcctgtatccgaacgcatttatcgaagtggtgcatcaggcggcaaacgatttgattgcccatgtggcacaaagcaagatttgaggtgtgtaaatgaactggtctgatttgaatttaatgcccgccatgcccgaaatcgtgctgctgtcgctgctggtgttattgttgctggcggacttgtgggtcagtgatgacaaacgcccgtggacgcattacggcgcgttggcaacggtggcggttacggctgtggtgcagttggcggtgtgggaacagggcagcacgtcttcgttcaacgggatgtatattgcagacggtatgtcgcgtttggcaaaaatggttttatatgccttgacctttgccctgtttgtctatgccaagccctacaaccaagtgcgcggtatttttaaaggcgagttttacaccctgtcattgtttgccctgttgggtatgagtgtgatggtgagcgcggggcattttttaactgcctatatcggtttggaactcttgtcgcttgccctttacgccctgattgccctgcgccgcgattccggctttgccgccgaagccgccttgaaatattttgttttgggcgcgctggcatccggcctgctgctctacggtatttctatggtttacggcgcaaccggttcgctggaatttgccggcgtgctcgcctcttccttcaatgaagaagccaacgaatggctgttgaaactgggtttggtgtttatcgtcgtcgccgtcgcgttcaaactcggtgcggtgccgttccatatgtgggtgcccgacgtgtatcacggcgcgcccacttctgttaccgccttggtcggcactgccccgaaaatcgccgccgtcgttttcactttccgcatcctcgttaccgggctgggaaccgtgcatcatgactggtctctgatgtttgccctgcttgccgccgcctcgctgctggtcggcaaccttgccgccatcatgcagaccaatatcaaacgtatgttcgcctattccaccgtatcgcatatgggtttcatcctgttggcgtttatggcgggcgcggtcggctttgcggcgggcctctattacgccattacctacgcgctgatggcggcggcagggttcggagtgttgatggtgttgtcggacggggacaacgagtgcgaaaacatcagcgatttggcagggttgaaccaacaccgcgtatggcttgcctttttgatgctgctggttatgttctctatggcgggcattccgccgctgatgggtttttacgccaaattcggcgtgattatggcactcttgaaacaaggccatgtttggttgtctgtatttgccgtcatcatgtcgctgattggtgcgttctactacctgcgcgtggtcaaagtcatctacttcgatgtgcctgatcatgaccagccggtcggcagcaactatgccgccaaatttgttctgacggtcaatgccttcttgctgctcctgtggggcatcatgccgcaaaccgttatcgactggtgcgccaaggcgttggagaacacgctgtaagccgccgcaacggcagccgtgtcagaggctgccgtttttgttaagatatgccgttccgcaacgcggttcagacggcatcgccgccgacaacgcctaaacagaaagcccaccatgaccgcatccatgtacatccttttggtcttggcactcatctttgccaacgcccccttcctcacgaccagactgttcggcgtggccgcactcaagcgcaaacatttcggacaccacatgatcgagctggcggcaggtttcgcgctgaccgccgttcttgcctacatcctcgaatcccgtgcaggatcggtacacgatcagggttgggagttttatgccacagtcgtctgcctgtacctgatttttgcgtttccatgttttgtgtggcggtatttttggcacacgcgcaacagggaatagacaagcataggaatgccgtctgaaaccctttcagacggcatttgtttcattcaagtgcaggccggcatcgctgtgccggcacgtttcagccggcgatatacgccggttttaatatttgcgggcgactgcaaattctgccaactgccgcaggcgcagggctttgtcgccgaagggttcgagcagcgcgaccgcttcggcaaccagtttgtgtgcgtatgagcgcgccgcttccaagcccatcagtttcacataagtcggcttgtcgttgtctgcgtctttgcccgccgttttgcccaaagtcgccgtgtccgcttcacaatccaacacatcgtcaatgacttggaacgccagccccagttttgccgcgtaagcgtccaatacggaaagttccgcatctgacagatcaggacacgccgtcgcccccaataaaaccgccgcacggattagcgcacccgttttcaggctgtgcatctgttccaaatcggcttgaaccatttgtttgccgacattcgccaaatcgattgcctgaccgcccgccatacccctgctgccgcccgctttcgccaacaccgacaacattgccaactggcgtgcggcgggcagttctgtcggacggctcaacacgtcaaatgcctgtgtctgcaaagcgtcgccggtcagaagggcggtcgcttcgccatatttgatgtggcaagtcggtttgcc

附录Bgcgccgcaggctgtcgttgtccatcgccggcatatcgtcgtgaaccaaagaatagacgtggatcatttcgattgccgccattgcctgttctactgcttcatgcacggcttcgcctaattccgaagctgccagaaccagcatcggccgcagacgcttaccgccgtccaaagccgcataacgcatcgcttcgtgcagtgtgtgcggtatttccccctcagacggtaaaaaccgttcaagcagcagctctgtttgcgcctgcgccctctgttgccacgttttcaaatcattcgtcggattcaaggtttaactccttcagcccgtctgtgtctaaaacctgtagcttttgttcgacttgtgccagtttggtttggcagtacctgaccagttcgttgccttcctgataggcggcaagcgcgtcttccaagggcatttcgccctgcatagactgcgtcagcgattcgaggcgcgacaaggcttcttcaaacgatttcggggcgtttttcttcatcgtatttccttttcggttgaaaccccgccctttagggcggcaggatcagactttatttgggaggggtgtaaccctttccaaatcagggcaatacatagggcggtgctttatgtgccgtcctgtgtgttggaacatagtttcggatgttccggtaaaaagcggattgtagcatttttgaaaaacggatgccgtctgaaacccgaatccggcttcagacggcattttttccgcccaggcggcaaggcgttacccgggcagttcgtcggtgatgccctgcaaaaaggcgaggcgttcggggcttgccgccccggtttgcgcggcggctttgaaggcgcagccgggttcggcgcggtgggtgcagttgtggaagcggcattgcccgacaaggtggcggaaatcggggaaatagcgcggcaaatcggcggcttggaggtggtgtaaaccaaattcttgcaaacccggggagtcgatgagttgggtttcgccgttcaaatcataaagccgggcgtgggtggtggtgtgttttcccgagtcgagtgcggcggaaatgtcgccggtgcgggcggtttggctgcccaaaagggcgttggtcagggtggatttgcccataccgctctgcccgagcaggatgttgctgtgcccttgcagggcggggcgcaggctgccggcgttttccagtgcgcgggtttcgatgacgggataacccagcgtttcgtagaatttgagtttttcgcgccaaagggcggtttcgggcaggtcggctttgttcaggacgatgacggcttcaataccggcggcttcggcggcaagcagggcgcgttgcagcagccgcacgctcggactcgggacggcggcggttacgatgaggagttgggtaacgttggcggcgatgagtttggttttccacgcgtcttggcggtagagcaggctttggcgcggtaaaaaatcttcaatcacaacttgttcggcgttgacggggctgatgcggacgcggtcgccgcaggcgaaatcgacgcgttttttgcgggtgctggcttcgtaggttgtgccgtcgggcgtgcggacaatgtagcggcggccgtagctggcggtaatttgggcggtgtcgttcatggtttctttggggttgggtgtgggaatgccgtctgaaaacgggtgttcggacggcatcggttcagtcgtgctgccactcgacgtgttcgttgaggaagccgccgctctggtgcgcccagagtttggcgtaaagcccgcgtttttcgaggagttcggcgtgtgtgccttcttcgatgatgcggcctttgtcgaggacgacgagcctgtccattgcggcgatggtggagaggcggtgggcgatggcgatgacggttttgccgtccatcattttgtcgaggctttcttggatggcggcttcgacttcggaatcgagcgcgctggtggcttcgtccaaaagaagaatcggtgcgtctttgagcatcacgcgggcgatggcgatgcgctggcgttgcccgccggagagtttcacgccgcgttcgccgacgtgtgcgtcgtagccgcgccgccctttggcatcggaaaggtcggggatgaagccggcggcttcggcgcgttcggcggcagaaaccatttcggcatcggtcgcgtcggggcggccgtaaataatgttgtcgcgcacggaacggtgcagcagcgaggtatcttgcgtgaccaaaccgatttgggcgcgtaaagattcttgggtaacgccgcttatgtcctgcccgtcgatcgaaaccgtgccgctttgcggttcgtagaagcgcaaaagcaggttgacgatggtggatttgcccgcgccgctgcgtccgatcaagccgactttttcgcccgggcggatggtgaggttgaagccgttgagcagcggtttgcccgcttcgtaggagaaatcgacgtgttcaaatttgattgcgccttgcggcacgttcagcggcagtgcccggggcttgtcgaggatggtgtgcggtttggacagggttgccatgccgtcgccgacggtgccgatgttttcaaacagccgcgcggattcccacataatgtattgcgacaaaccgttgacgcgcaacgccatggcggtggctgtagcaaccgcgcccacgccgacctgcccgttgtgccagagccagatgcccagtgcggcggtggagagggtcagggaggtgttgacgatgaagctgcacgaatgcagcagcgtcgccagccgcatttgggcgcgcaccgtaaccataaattcttccatcgactgcttggcataggcggcttcacgcgcgccgtgggagaagagtttgacggtggcgatattggaataggcatcggtaatgcggccggtcatcagcgagcgggcatccgcctgccatgcggcggtttgccccaatttgggaatcagcaggcgcatcaccgaagcgaaaccgacaatccagccgataaagggcagcagcagccatgagtcgagcgaggcgagaatcacgccggaggtaatgaaatacaccgacacataaacgaccatatcggcaaccgtcatcaccgcgtcgcgcaacgccagcgcggtctgcatgactttggcggacacgcgtccggcaaattcgtcctgataaaaaccgaggctttggttcagcatcaggcggtggaagttccagcgcaggcgcatggggaacacgccctgaagggtttgcaggcgcacgttggacgcggcaaacgcccacgcaaccgaaaataccatcatcgccgccattgccgccagttcccaacttttttcggcaaacagttcggcgggcgcgtatttgccgagccactccacgattttgcccataaattgaaaaaccagggcttccataatgccgatgccggcggtcagcgcagccagggcggctatccatttccgcacgccggccatgctgctccagacaaaccgccacaagcctttttctggcgttttcggggcggcttcgggataagggtcgattcgggactcgaaccaggaaaatattttgttcaacattgttttcgatttcggtaaaacagtttcagacggcatcaaacacaatgccgtctgaaaggaaggacaataacgccattttacgggaaaagccgtcgggaagacagcgcgaggcggaaacgcagggtttcgtcagggcaaacgccgcgccgccttcaggcggcattatttcagcaggtttttcaaagcaaggcgcacgccttcgcccacgtccgtcccctccggaacgcctttgaccgccgcttttgcttcgcgttcgctgtaacccagcgcaagcagcgtgctgacgatgtcttccgtttcgtcggcggcgggtgcggcggcaaacagcccgtccgttaccgtatgcgcgaccagcttgccgcgcagttccaaaaccatacgttcggcggtttttttgccgattcccggggcggaggagaggcgtttgacatcttcttctgcaaccgcccgcgccagttcgtcggcagtcattgccgacaaaatgcccaaagccgttttcgcgccgatgccgccgaccttgatcagttggcggaaggtcttgcgttcttccgcagtggcaaaaccaaataaaagatgtgcgtcttcccgaatgataagctgggtaaacagttgtacgctttcacccacgggcggcaggttgtagaaggtctgcatcgatacgtcggcctcatagccgacaccgttgacatcgatgacgatttgcggagggtttttttcaaccagtttgccggtcagtctgctgatcatgtgtgccgaatcctgaagtgtcgggtgcaaaatgccgtctgaaaccggtttgggcttcagacggcacggattgtatcaaattcagtcgtcgcggcgggaggaaatcacgcggccggtacgggcatcgacaacgactttgtattcctgtccgtttttgacgatttcgacatcatagtgcggacggccgttgtcgtgttcgagatcgatgtcggtgattttgccgccgacacgcgccaacgctgctttttcggcttgggcgcggctgatgattttgtcttgtttgttgtgttggtgtgcggcgtgtccgtggtcgtcatcgccgtgtccgtcgtggtgggcgagcgcgggggcggaaatgctcagcagtgcggttgcggcggaggtcaagagaaggtgtttgatgttcatattttgcctttgtaaatcgtgggttggaaaatgtggatattaataaggtatcaaataaccgtcagccggcggtcaataccgcccgaaccataccgcgcgcctgagcttcggcttcggcggcgcgttcctgcgaggtaaacggtcccattttgacgacgtattcgtaacggcgtttttcaaccgagaggttcgtacccgatgacgaaacggcgaagttttgggcggcttggttcagataggcttgtgcttcgtgttccgtaccgaaagatttcaagtcgataaagatgtctttgttttcggcaaccggtgcggattggcccgggacgatttgttcgattttgacgtgtgccgtcccttggttgacaaagcccaatttttgcgcggcggctttggatacgtcgatgatgcggttgccgtggaaggggccgcggtcgttgacgcggacgatgacgcttttgccgtttttggtattggttacgcgcacatagctggggatgggcagggttttgtgggcggcggtaaaggcgttcatatcgtatcgttctccgccggaagttttgcgcccgtgaaacctgccgccgtaccacgaggcgttgccggtttgcgtgaattcggcgacttggtttttcggcgtgtagcgttttccggcgactttgtagctgcggttggcggaggcgtgcagtttttctgccttgaccactgcgtcggcggatgccgtctgaagggagtgtgtgccgaatgcggcggtgagaaggaaaagggtttttcgggttaaagtcaaaacgtgttccgttcttgagttgaagacgaatgggcatcatgcccgccggatacgttccgaaccgccgtacagtgcggacggcggttcggaatgtgtccggataggttttcagacggcatgaacctgcgttcaaacgccgcctgcgtaaccgtgttgccgccacgcttcaaagagaatcacggcgacggtgttggaaaggttcatactccggctgccgggctgcatcggcaggcggattttttgcgcggcgggcaggctgtcgaggatgtcggcaggcagtccgcgcgtttccggcccgaacagtaaaacgtcgcctttttgaaacgcggtttcatcggggcgcgccgtgcctttggtggtcagggcgaaaatgcgcctgcctgcgagtgccttgaggcagtcgtcgaagttttcgtgcaccgtcaggctggcgaactcgtggtagtcgagcccggcgcgtttcattttggcggaatccaatgggaagccgagcggtttgacaaggtgcaaatccgcgccggtattggcgcacaggcggatgatgttgcccgtgttcggcgggatttccggctggtataaaacgatggtaaacataaatatcaatcacttataggcgcgtaaccttgccacaaggcggatggggtgtcaaaaaatttagttattttttcattggcgtgcgtgccagcgtccagcagcagattcggtttgcgcccgattttttcagcgtctttgccaattcgtccagcgtcgcgccggtggtaaagacatcgtcgattaacagaatattacagttttccggtatcggtgtgcggatttcaaaggcgtttttgatgtttcgccgccgttcgccgcctttgagcgtgctttgcggcgggcggtggtgtcggaaaacggtgtgtcggggcagtatctgccagccgtagcgttgtgccagcagcccgacgatgctttcactttggttgaacccgcgttgcagcagccgctccctgcttagcggtacgggcaggacgaaatcgaaacattcgtctgcaagccggtcgggcggattctgcatcatcaggtctgccagcggctgcaccatgctcaaatcagccaagtgcttcagcgcgtgtatcatattgctgacgggcggttcgtaatgcagcgaagcccacatccggtcgaatgcgggcggttttttctgac

附录Bagccgccgcacaccgatccgccttggatgtgtctgaaacacagggggcagctgtttgccgcgtcggtgcggtatgccgccaaatcgtcgcggcagccggcgcagatgccgtctgaaacgccagacgaaccgtggcataatacgcaacgcctgatagtgggcgcgtctgcgatgcgccgccaacgagagagaaaatccatgcctgatgccgtcaaaaaagtttacctgatacacggttggggggcgaaccgccacatgttcgacgatttgatgccgcgcctgcctgcaacgtggccggtgtccgccgtcgatttgcccggacacggggacgctccgtttgtccgacctttcgacattgcggctgcggccgacggcattgccgctcaaattgacgctccggccgacattctcggctggtcgctcggcggattggtcgcgctgtatctggcggcgcgccatcccgacaaagtccgttcgctctgcctgacggcgagtttcgcacggctgacggctgacgaagactatcccgaagggcttgccgcgcctgcattgggcaaaatggtcggtgcgttccgttcggattatgccaaacatatcaaacagtttctacaattacagcttctgcacacgcctgatgcggacggaatcataggcagaatcctgcccgatttggcgcgctgcggcacgcctcaagccttgcaggaggcgttggacgcggcggaaagggcggatgcgcggcatttgttggacaagatagatgttccggtactgctggtgttcggcggcaaagacgcgattacgccgccgcgtatgggtgaatatctgcaccgccgtttgaagggcagcaggttggttgtgatggaaaaggcggcgcatgcgccgtttttgagccatgcggaagcgtttgccgcgctgtaccgcgactttgttgaagggggtttgagatgaaccatcaggacgcacgctggcaggttcaccgccatcttgccgaacataccgaccaacggctgacactcgtccgcaacgcgcccaagcatatcctgcttgccggtgcggatgcggacatcagccgcagcctgctggcgaaacgctatccgcaggcggtatttgaagaatacgattcccgtgcggattttttggcggctgccgctgccgcccgcaaaggcggtttttggcaaaggtttacgggtaagggcgtggtgcaacactgccaatccccgatcgcgccgctgcccgaagcgtgtgccgatatgttgtggtcgaatctcggactgttggcggcggaacaaatccttcctgtgctgcacaactgggcgcgcgccttgaagacggacgggctgctgttttttacctgcttcgggcgagataccttggcggaactgaaatgccgtctgaaagaaaacggcattgaaagccgcagcgcgcttttccctgatatgcacgacttgggcgatatgcttgctgaaaacggcttttacgaccccgttaccgatacggcgaagctggtgttggattacaaaaaggcggaaacgttttgggcggatatggacacgctgggcgtttggcgggcgatggcgtggaacgatgaaaacgccgcgcgttcgtgtgtcgggacaatatttgagcgggaaggcggtttgggcattacgctggaaacggtgtacggacacgccgtgaaaaaactgatgctgccgcaaggggagaacgtggtgcagttttttccgaagagatgatgtgcagatgccgtctgaagccgtttccaggtttcagacggcatttgtctgtgaaaaccgacagaaataaaggaaatgccgatgtatagtgaattaaatttaaaccagtacagcgttgcctcgccttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttccgtactatttgtactgtctgcggcttcgccgccttgtcctgatttttgttaatccactatatgctgatgccggagacgtatattgcgtctataacatcagactgaagcagtacactgcctgccaggttacccgagttgaagaacacggtggcaaaaaaaacacatgcgaccctgctggctttggactggcagggcaacaaaccgcttggggcggaggagctggcggatttgaaatcgctttacaaagacttaaagaataatattggaaatattgtatgaacaaaaaattaaactatatttttatgttggactgtttagggttggtgatattgtttacttgtataatagctacttttgaaagagattatggatttaaaatttttactaattctaagagacctgaattttattattggattggaatgttttattatggaattatttcttgctggtttgattatcaattaatttcaacaaaggcgaattcgtataaaagaaaagttaaacaatataaaattttttcagtaatattttcagttttgatatttatttctactatagtaaaactttaaattttggagcaaaaatttatgagcgattcaattgaatatgtattgggaacgcggtctgcacatgtataaggcaagtgccgtcgtgccgacgggatatgtacgggttgggaataccgcgccgctggtcggcgaagacacgcaacggtatgcctctttttggggcgacggctacgacgtgtaccgtcagttgagatggcagcagatacccgaaaaacagagaaaggcattcaaaaaagccgccaaaagcaaaaagaccgtgatgtttgccggacgggaatacggcatatccaaacagaatttgagcgatgtttgggatgattttgaagacgcgatggaactgaaggcgtttccctgcctgtcttcgctgtttctgaccaaatggcataaaaatctatatgatagtggattaacaaaaaccagtacggcgttgcctcgccttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttccgtactatttgtactgtctgcggctcgccgccttgtcctgatttttgttaatccactataaaaacaggaatttttaaatagaggcaatgccgtctgaaacttggtaacgggcttcagacggcatttcgttccaataccgccaacaccgccgcaccgtaacgtgcggctttttcttcgcctacgccgtatacggcggcaagctccgccaagccttccggctgtttggcggcaatggcgcgcagtgcggctttgctgagaatgcggtagggttcggactgttcgtgttttgccgtttcgccgcaccattggatcagggcgcgcatcaggcggcgtttgcgtttggcggtttcatcgatgccgtctgaaaacggacggcagacggcgaggatgtcccgtccgtatttggcggcgcgtacgctgcccaagccgtacacgccttcgaggtcggtttcggtttcgggcgtatcggcaagcatatcggcaaggctttcgtcggagaggacggcatgcagggcgcagttttccgcccttgcctgttcataccgccaggcttcgagtttttgacgcagttgttgttcgcgttcggtttgcggacggatgaccgcgtcgcggctgaagccggcggcgttgcggcagacttcgaggatgccgtgtccgaaacggtcgattttggcttcgcccaaaccgtagatgtcgtgcagaccgttgaggtcttgcggcattttttcgacaaggtcgcgcagggttttgtcgccgaaaatcatataggcggggatgccttcggcttctgcctgtttcatacgccaaacgcgcaatgcctgccacaggcgttcttcgcgttcggtacgcagccagttgtctttgagggtgcgggcggcgggcttgtcgcgcttgagcggacgcagcatcacttcggtttcgcctttgaggacttttttggcggcttcggtcagttgcaatgcctgatatcgggtaatgttgacggtgaggtagccgaggctgatacactggcggatgacgctgcgccattctttgtcggacaactccgtaccgatgccgaatgtggacagttgttcgtgccggttgccgcgtatccaatcgtcgcttttacctcgtaaaatgttggtgatgtaaccggcggcaaaacgttgtccggcgcggtacacgcagctgagtaatttttgcaccaacaccgtgccgtcaaaccgtacgggcggatgcaggcagttgtcgcaatggccgcagggttcggatgcttcgccgaaatgtttgagcagcagtacgcggcggcaggcggcggtttcgcagacggcaagcatggcatcgagtttttgcatttcgatttgcttttgcacctcgtcgctgttgccttcggcaatccgttcgcgcagcaacacccaatcgttcaaaccgtaacacagccagcttgcggccggcagcccgtcccgtccggcgcgccccgattcttgatagaaatgttcgacactctggggcatatcgagatgggcgacaaagcgcacgtcgggtttgtctatgcccatgccgaacgccacggtcgccaccacgataatattgtcttcatgcgtaaagcggcgttggttttcctcgcgtacgtccatgctcaaaccagcatgatacggaatcgcgtttaatccgttttcacgcaaaaactgcgccacatcttccacctttttgcggcttaggcaatacacaatgccgctttgccccgtcatttctttgcggatgaaatccagcaattgttttttgccgttgtttttttcgataacctgataataaatattcggacggtcaaagctggagacaaattcgggcgcatcgtccaagtgcagataatgcttgatgtcggcgcgcgtggcggcatcggcggtagcggtcagagcgatgcgcgggacgttcggatagcgttcggcaagcatgccgagctgttgatattcagggcggaaatcgtgtccccattggctgacgcaatgcgcctcatcaatggcaaacagactgacggtttgttggtcgagaaaacgcaaaaagcggtcggtaaccaagcgttccggcgcgacataaagcagcttcagacggccttgggcaagccggtcggcaatctcgcgcgcctcgtctgccgatgtgccgctgttgactgccgccgcttcgatgccggcggcgtgcaggtttgccacttggtcgttcatcagcgcaatcagcggcgatacgacaaccgccacgccttcgcgcatcagcgcgggaatctggtaacacaaagacttgccaccgcccgtcggcatcagcaccgtcaaactcccgccgcctgccaaagtattgatgacagcctcctgcctgccgcgaaattcgggataaccaaatacttcgtgcagaatctgtttggcggtcggtcggtgcatgatggttccgtgctcggtaagggtgttgatcggtcggcggcaatatgccgtctgaaatcgggatttagaatagtttgcccacttctgcttcaatatcgtcggcacgcataaacgtttcgccgatcaggaaggtatgcacgccgcgcgattgcataaattccacatccgccttgcctgtaatgccgctttcggtaacgacggttttgccttccagcgcgggcagcagcgacagggtttggtcgagggagacttcaaaagtcctcaggttgcggttgtttacgccccacagcggcgtggtcaggttgcggcatttttccaattcggtttcgtcgtgcagctcgagtaggacggtcatgcccaattcgtgcgccaccgcttcaaagcgttccaattgttcctgttccagtgctgcggcaatcagcaggacggcatccgccccccatgcgcgcgcctgataaacctggtattcgtcgatgatgaagtctttgcgcagcacgggcagcgatacggcttcgcgcgcctgtttgaggtattcgggcgaaccttggaaatagggttcgtcggtcagtacggacaaacacgccgctccggcgttttcataggcgcgtgcaatctcggcagggcggaagtccggacggattaaccctttgctcgggcttgcctttttgatttcggctatgacggcgggcaggtttaggcggtgtttgccgcgtatcgaatcgatgaagctgcggacgggcgcggcttctgcggcaagtgtgcggatgtgttcggcgttgacggcggctttttgagcggcaacttcctgtgctttggtggcaaggattttattgaggatgtcggtcatgtcgggttccgtattcgtctggggaaagggggaatattagcatcaaaccgttaacgcctgtttgtgcggaagctgtcgaaataggacaggacggtctgcggcagccattgcaggtgcagcctgccgccggtgctgctgacaaagccgacatgaccaccatatgccggctggaacagggtaacggcttcggatacttcgtctgcgcggggcagggcttcgggcggcaggaaggggtcgttgacggcattgagcaggagcagcggtttggcaacgtgtttgagcagcggtttgcaggaagtttggcggtagtagtcgtgccggtcggcaaagccgtgcagcggtgcggtgaagcggtcgtcaaactcgcccagtgttttgcaccctgcggcaaatgccgtctgaaaaccttggagcgattttgcttt

附录Bgggtatcagggtgcggaggaagtagcgcgtgtagagcagccgcgtgatgccgctgtcgaagcgtctgcctgccgcctctgcatcgacgggggcggagatgacggcagcggcttgcggcaatgcctttttgccctgttcgcccaaatattttgccagcgcgttgccgcccagcgatacgccgacggcgtatatttcacggtaacgcgcggcgaacgtgtccaaagtaaaggcgatttcggcggtatcgcccaagtggtagaacaccggagcggtgttggcaatgccgccgcagctgcggaaatggacgactacgccgtgccaaccccgatcgcgtaccgcaagcatcagttcgaccgcgtaatggctgcggctgcttccttccaaaccgtgaaacagcacgaccagcggcgcatcgggcgaaatgccgtctgaaaagtcgtaggcgactttggttttacccgtgctgtcgggaagcagctctcggcggtatgcgggcgcggggcgttgcaggaatttggcggcaatcgtgtcggcattgccgttgcggaggaaaaagggcgtgtccggcggtgttaaaatcataaggtatcggttttcttgttttcagacggcattgatgatgcggcagcccgtccggctggtgcggacgtgggggatgcgcgcccgaatataggcgtggaaaagcgtttgccgaaaaaggatatcggcatcggtcagttttccacgcgtttgaaatggcgcggacggaagcccaaagccgccagtgatgcgaaatacagtccgccgccgacggcaatcaggatgcagagctgccccgctttccgcattccgccggcgtgcgcccattcaaacggcaggtaagcctgcgctgcccacagtccgccgcacatcacggcgagcgagagcagcatttttgctaagaacgctgcccaacccttgccaggttggtaaataccgtgtctgcgcaacaggtaaaacaacaatccggcattgatacacgcgcccagaccgatggcaagcgaaagtccgacgtgtttcagtgggccgataaaggcaaggttcatcaactgcgtgcagatgagcgtgaagatggcgattttgacgggcgttttgatgttttgccgcgcatagaagccgggtgccaacactttaatcatgattaagccgattaaaccgaaagaataggcaatcagcgcgtgttgcgtcatctgcgcgtcaaacagcgtaaattcgcggtacataaacagcgtcgccaccagcgggaacgacaacaccgccagtccgaccgccgccggcagcgtcagcagcatgcacaggcgcaaaccccagtcgagcagggcggaaaactgttccgtatcttggtttgccgagtgtttggacaaagtcggcagcaaaatcgtaccgagtgccgcccccagcacgccgctgggcagctccatcatgcggtcggcgtaatacatccatgaaacgctgcccgattgcagataagacgcgaaaatcgtgttgatcaccaaagaaacctgcgccacgctcacgcccaaaatcgcaggcgccatctgtttcatcacgcggttgaccgccgcatctttgaaactcagtttgggcagtttcaaaaagcccagtttcgccagccagggcagttggaagccgagttgcaaaatgccgccgacaaagaccgcccacgccagcgcggtaacgggcggatcgaaatacggcacgaaaaacagcgcgaatacgataaacgacacgttcagaaacgtgggcgtaaacgccggaatgccgaacttatgataagaattgagtaccgagccgacaaatgaagacagggaaatcaataatatataaggaaacgtaatccgcagcaaatcgatggagagctgaaatttgtcggcatcttgggcaaaaccgggtgcggaaacataaatcacccaaggcgcggcaagtatgcccagcgcggtaacgataaccagtacaaacgacagcatccccgccacatggcggataaaagcctccgccgcctcttttgaacgcgtttccttgtattccgccaaaatcggcacaaacgcttgggcaaacgccccctccgcaaacacgcggcgaagcaggttgggcagtttgaacgcgacaaaaaacgcatccgtcgccatacccgcgccgaatgcccgcgcaatgaccgtatcgcgcacaaatcccaaaacgcgcgacaccatcgtcaggctgccgacttttgccaaagctcccagcatattcatcattgttcctcaacagtcgtacccgtctggggcaacggcgcgtattgtacgacagaaaccgcttcagacggcatcgggtttgatgccgtctgaagcggtttcctgaaacgaaaacgtccttttccggcggcaaactgtatcaatacgcggaaatgcaataaaatagccggattccgattgatttccaacatctgtttccaacatcacggagaaccgtatgaaatccagacaccttgccctcggcgttgccgccctgttcgcccttgccgcgtgcgacagcaaagtccaaaccagcgtccccgccgacagcgcgcctgccgcttcggcagccgccgccccggcagggctggtcgaagggcaaaactataccgtccttgccaacccgattccccaacagcaggcaggcaaagtcgaagtccttgagtttttcggctatttctgtccgcactgcgcccacctcgaacctgttttaagcaaacacgccaagtcttttaaagacgatatgtacctgcgtaccgaacacgtcgtctggcagaaagaaatgctgacgctggcacgcctcgccgccgccgtcgatatggctgccgccgacagcaaagatgtggcgaacagccatattttcgatgcgatggtcaaccaaaaaatcaagctgcaaaatccggaagtcctcaaaaaatggctgggcgaacaaaccgcctttgacggcaaaaaagtccttgccgcctacgagtcccccgaaagccaggcgcgcgccgacaaaatgcaggagctgaccgaaaccttccaaatcgacggtacgcccacggttatcgtcggcggtaaatataaagttgaatttgccgactgggagtccggtatgaacaccatcgaccttttggcggacaaagtacgcgaagaacaaaaagccgcgcagtaagcccgtttgaaaaatgccgtctgaaacttggttttcagacggcattttgattgggtttaaaacgtaaagcccgtttccagttcttcatcgccgaccagttcgaccaagagcgcgtagagcggggcgagttcggcataacggcgcgatacgcggcgcagatagtttaagaaacgcgggatttccggacggtatttgtctttgccgtcgcggtagtacaggcgtgcgaagatgcctgcaaccttcaagtgccgctgcacgcccatccattcgaaccagcggtaaaactcgtcaaacgcttcggggacgggcaagccggcagcccgcgccttttcccagtagcggataaccaagtccaagacaaattcttcttcccattcgataaaggcatcgcgcaacagcgacaccaaatcgtaggaaatcgggccgtaaagcgcgtcttggaagtctaaaacgcccggcctgccgcgcgtcagcatcaggttgcggacgataaagtcgcggtgcacatagactttgggctgcgccaacaggggcggcagcagcgtatcgacggtttgctgccaaagttggcgttgtttgaatgttaattcgcgccccaattcttttgcgacaaaccattccgggaacaggttgatttcgcgcaacatcgtttcacggtcatattcgggcaaaaccccttcacggctcgccttctgcaattcgaccaactcgccgattgcctccaaaagcagggctttgtgcgccgtttcgccctgttcctgaagcattgcggtcaaaaacgtcgtattgcccaagtcgttcaataccacaaaccccagatccgtgtccgcgtgcaatacctgcggcacattgaccatgtcaaacagtttctgcactttcaaataaggtgcgacactcatcttgtcgggcggtgcatccatgcagacgacactgctgccgtctgaaaacgttgcacggaaatagcggcggaaatcagcatccgccgccgcaaaagtcagatcgaagtcccgttcgggataaacggtctgaagccaatttttcagtttgatttgtcgttgcataacagtactaaagcatttcaggttacaataaacgctattctaactggcaaaccgacttgaggggcgattttggctcgtttattttcactcaaaccactggtgctggcattgggcctctgcttcggcacgcattgcgccgccgccgatgccgttgcggcggaggaaacggacaatccgaccgccggagaaagcgttcggagcgtgtccgaacccatacagcctaccagcctgagcctcggttcgacctgcctgttttgcagtaacgaaagcggcagccccgagagaaccgaagccgccgtccaaggcagcggcgaagcatccatccccgaagactatacgcgcattgttgccgacaggatggaaggacagtcgcaggtgcaggtgcgtgccgaaggcaacgtcgtcgtcgaacgcaaccggacgaccctcaataccgattgggcggattacgaccagtcgggcgacaccgttaccgcaggcgaccggttcgccctccaacaggacggtacgctgattcggggcgaaaccctgacctacaatctcgagcagcagaccggggaagcgcacaacgtccgcatggaaatcgaacaaggcggacggcggctgcaaagcgtcagccgcaccgccgaaatgttgggcgaagggcattacaaactgacggaaacccaattcaacacctgttccgccggcgatgccggctggtatgtcaaggcagcctctgtcgaagccgatcgggaaaaaggcataggcgttgccaaacacgccgccttcgtgttcggcggcgttcccattttctacaccccttgggcggacttcccgcttgacggcaaccgcaaaagcggcctgcttgttccctcactgtccgccggttcggacggcgtttccctttccgttccctattatttcaaccttgcccccaatctcgatgccacgttcgcgcccagcgtgatcggcgaacgcggcgcggtctttgacgggcaggtacgctacctgcggccggattatgccggccagtccgacctgacctggctgccgcacgacaagaaaagcggcaggaataaccgctatcaggcgaaatggcagcatcggcacgacatttccgacacgcttcaggcgggtgtcgatttcaaccaagtctccgacagcggctactaccgcgacttttacggcaacaaagaaatcgccggcaacgtcaacctcaaccgccgtgtatggctggattatggcggcagggcggcgggcggcagcctgaatgccggcctttcggttctgaaataccagacgctggcaaaccaaagcggctacaaagacaaaccgtatgccctcatgccgcgcctttcggtcgagtggcgtaaaaacaccggcagggcgcaaatcggcgtgtccgcacaatttacccgattcagccacgacagccgccaagacggcagccgcctggtcgtctatcccgacatcaaatgggatttcagcaacagctggggctatgtccgtcccaaactcggactgcacgccacctattacagcctcaaccgcttcggcagccaagaagcccgacgcgtcagccgcactctgcccattgtcaacatcgacagcggcgcaacttttgagcggaatacgcggatgttcggcggagaagtcctgcaaaccctcgagccgcgcctgttctacaactatattcctgccaaatcccaaaacgacctgcccaatttcgattcgtcggaaagcagcttcggctacgggcagctctttcgcgaaaacctctattacggcaacgacaggattaacaccgcaaacagcctttccgccgccgtgcaaagccgtattttggacggcgcgacgggggaagagcgtttccgcgccggcatcggtcagaaattctatttcaaggatgatgcggtgatgcttgacggcagcgtcggcaaaaaaccgcgcaaccgttccgactgggtggcatttgcctccggcagcatcggcagccgcttcatcctcgacagcagcatccactacaaccaaaacgacaaacgcgccgagaactacgccgtcggtgcaagctaccgtcccgcacagggcaaagtgctgaacgcccgctacaaatacgggcgcaacgaaaaaatctacctgaagtccgacggttcctatttttacgacaaactcagccagctcgacctgtccgcacaatggccgctgacgcgcaacctgtcggccgtcgtccgttacaactacggttttgaagccaaaaaaccgatagaggtgctggcgggtgcggaatacaaaagcagttgcggctgctggggcgcgggcgtgtacgcccaacgctacgttaccggcgaaaacacctacaaaaacgctgtctttttctcacttcagttgaaagacctcagcagtgtcggcagaaaccccgcagacaggatggatgtcgccgttcccggct

附录Batatcaccgcccactctctttccgccggacgcaacaaacgaccctgaccgtcggaaacctggcaggagcaccgttcccgcacaagacggcattccaccgacaaccccaaacccgccatcaaaggcaggattcaaacgataaggaaagaatgatgaaaatcaaagccctgatgattgccgccgcattgctggcagcagccgatgtccacgccgcaccgcaaaaggcaaaaaccgcatccgccaaagctgccaaagctgccaaagctgccaaagttgccaaagttgccaaagttgccaaagttgccgccacggcgcaaaaagaagccgcacccgcacaacagcagggcggtatccgcttttcagacggcattgccgccgttgccgacaacgaagtcatcacgcgccgccggcttgccgaagccgttgccgaagccaaagccaacctgcccaaagacgcgcagataagcgaatccgagctgtcccgacaggtgctgatgcagcttgtcaaccaatccctgattgtacaggcgggcaaacgccgcaacattcaagcaagcgaagcggaaatcgatgccgtcgtcgcaaaaaatcccgccctcaaaaacctcagccccgcccaacgccgcgattttgccgacaacatcattgccgaaaaagtccgccagcaggcagtgatgcagaacagccgcgtgagcgaagctgaaatcgatgccttcctcgagcaggcgcaaaaacaaggcatcaccctgcccgaaggcgcaccgttgcgccaataccgcgcccaacacatcctgattaaagccgacagcgaaaacgccgccgtcggcgcggaaagcaccatccgcaaaatctacggagaggcccgcagcggcacagacttttccagcctggcgcgccaatattcgcaagacgcgagcgcgggcaacggcggagatttgggctggtttgccgacggcgtgatggttcccgcctttgaagaagccgtccacgcgctcaaacccggacaggtcggcgcgcccgtccgcacccaattcggctggcatatcatcaaattgaacgaagtgcgcgatgccggcacacctcaggaacgtatccgcaattccgtgcggcaatacatcttccaacaaaaagccgaacaggcaaccgtcaacctgttgcgtgacctgcattccggcgcgtatgtcgacatccgctaaggcggtttgaagcaaaaagccataccgatcggcaaaaatccgggcggtatggctttttggatttcgagttacttttacaccgtcattcatcattcccgcgaaagcgggaatctagaaacgaaaagtaacaggaatttatcgggaatggctggagtttaaaggactggattcccgccgtcgcgggaatgacgggattttgggttgtggtaatttatcggaaaaacaaaaaaacctatgccgtcattcccgagcaggcgggaatccggttatttaaaactgcagaaatttatccgaagcaacaacaatctttccatcgtcattcccgcgtaggcgggaatctaggacgtagaatctaaagaaaccgttttatccgataagtttctgtaccgaagaatctggattcccgctttcgcgggaatgacggcgcataagttcccgtgcggacagacctagattcccacctgcgtgggaatgacgattcagaagttgcctgaaacctaaaaaactgaaaccgaacgagccggatttccgctttcgcgggaatgacgggattttgggttgtggtaatttatcgggaaaacggaaacccctatgccgtcattcccgcgcaggcgggaatctaggacgtagaatctaaagaaaccgttttatccgataagtttctgtaccgaagaatctggattcccgctttcgcgggaatgacggcgtataagttcccgtgcggacagacctatattcccacctgcgcgggaatgacgattcagaagttgcccgaaaccaaaaaactgaagccgaacggtctggattcccgctttcgcgggaatgacggcgcataagttcccgtgcggacagacctagattcccacctgcgtgggaatgacgattcagaagttgcccgaaaccaaaaaactgaagccgaacggtctggattcccgctttcgcgggaatgacggcgcataagttcccgtgcggacaggcctagattcccacctgtgtgggaatgacgattcagaagttgcctgaaacctaaaaaactgaaaccgaacgagccggattcccgcttttacgggaatgacgggattttgggttgtggtaatttatcgggaaaacggaaacccctatgccgtcattcccgcgcaggcgggaatctaggacgtagaatctaaagaaaccgttttatccgataagtttctgtaccgaagaatctggatttccgctttcgcgggaatgacggcgcataagttcccgtgcggacagacctagattcccacctgcgtgggaatgacgattcagaagttgcctgaaacctaaaaaactgaaaccgaacgagccggatttccgctttcgcgggaatgacgggattttagattgcgggtatttatcgggaacggcggcttggaagttcattgaaacggaaaaacaacggaaacccaaaaaaccggattcccgactgtgggaatgatgagattcaggtttctgtttttgccggagtttgccgtatcgggcttcagacggcattgcctgccgttgtacccgcgggtgcgactgccttgatgtagttgagcgagacaaactgcttctcggcatccaattcggtgattttgaacaatgcctgtgatttgggcagtgcgtcaaacggaataccggtcgcgcgcgtgaccagcggcaggccttcgatgcggacgaggtcttctttgaggatggtcgcggtcagctcgcttgtaccttgctgttgcaggtacacaaggctccagtaggcttccatctgccgttggaaatcggcgtaggcggtataggcggcatcaaagtcgcgcagtgcggcgaaaagctcggcatcgctgttttgatacagcggctcggcagtgtcgtctatcaggctgatcagctgcttttggttgatgtagtcggcggcgcggcgcagcggcgaggtaaaccagccgtaatgctgcacgcccatgccgatatgcggctcggatttggtgctcatgcgtacttttccggtgggttggacgcggaagaggccgggcaggtcgttgtcatggagcatttgtgcccaagtgctgttggcaagaatcatcatctcgctgaccagcgtatcgatgggtgagccgcgttcgcggcggacgacggataccttgccttcctcatccaattcgatgctgtaatcgtattgcggcgcgcggtcgggttcgtatttgccgcgcgctttttgcagggcggtggcgaattgatagaaccaaatcaggtcttgatggtgggcgaacatcatttcgccggcttcgtccaagccggtttcggcgttgaaatgcggctcgatggcttggatacgcaggtttgtggcgatgttgaccgcttcgattttgcaggtcggcgcgccgacgttgaactcgccgtccacatcgaaataaatgctgacggcagggcggtgtgcgcctgcatcaaggctgaacgcggcaatccagttttcgggcagcatcgtgattttgccgccggggaaataaaccgtgctcaagcgttccatgatgtttttttccattttgtcgcccggtttaacggcaagtgacggcgcggcgatgtggatgccgacacgcttcgtgccgttgtccaagtcggtcaggcttaaagcgtcgtccacttcggtggttgattcgtcgtcaatggaaaaggcggtaacgtcggccttgggcaggtcgggcatttcgggaagggcaaggtcggggaagcctgttcctttagggaagtatttgatttcaaacccgtcttgcaggtattggggaatggacgtaatgccgcccgtttttttcgccaattcgtaggcagaggttttcagcgcgtcggcggctttggtaaaggctttgtaggtcagcgactgcttgtcgggcgcgtgcaggatggttttcaaatccgccgcgatttcagacggcatctcgccgcgtttcaaggcttctgcccaagcgtcgatttgcgcgtcttgctgttttttgcgttcgatggcggcaagtgcttgttttaaagtttcttcgggcgcggctttgaacacgcctttggcttttttgtagaaatacatcggcgcggcgtaaagcgcaatcaaagttgccgccagctcggttttggtcggcgcatggccgtaatattcttcggcgatggcttcggcggtaaattcctcttcgccgcatacttcccacaataaatcggtgtcgatgtccgccgcctgtgcctgcgcgttttccaaaaacgccgccatatcgccgtcaaactcggcaaagacgttgttcgccttcactttggtgcgtttgccgtgtggggtatcgacttggtaggtggcatcgtttttttggatgatggcggcgattttgaattggccggactcttcgtaaaaaatattcatttttcggatttttctgtggaaactcaagcgggcgattttagcagattaccgaaaatgccgtctgaaaaaaggttgggagagggttggcgcggctttgcggtgcttgcgttatagtggattaacaaaaaccagtacggcgttacctcgccttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttccgtactatttgtactgtctgcggcttcgtcgccttgtcctgatttttgttaatccactatacgtttttgacggtgtacaatcgctgtttttgaacggaggatggaatggagaatacaaaccgtgtgccggagcaggcacgttatgatgccgaacgcaggcaggcagacgaagcattggcgggcgtgtttccggcagtcagtatcttcggcagcgcgcgcacgccgcagaatcatgcggattatgcgttcgcctgccgtctggcgcggcggctgtcggattcgggcattgccgtcatttcgggcggcgggccggggattatggaggcggcaaacaagggcgcgtttgcagggaagtcggtttcggtggggctgaacatcgttttgccgcacgagcagaaaccgaatccgtatcaggacatcgccttgcggttttcccgttttgccgaacgcaaggcggtgtttttccgctattcccaagcatatgtcgtgatgccgggcggcttcgggacgctggacgaattgtttgaaatcctgaccttggtgcagacgggcaaagtgccgccgcgtccgattgttttggtcggaaaggcgttttggtcgggcttggcggagtggataaacgcgcagcttttggcgcgcggtctgatttccgaaggggcggtctctttgtttgccatatcggacgatgaagacgaaatcgttgcgtatctgtcggaacacgggcttcagacggcatagcgtcctgagagtgatgtataattgcaaacaatttaacaatttttgatgtctttcccgaacaggatgccgaaatgatcaaccccatcgcctcgctttcccctttagatggccgttatgcccaatccgttgaagcattgcgcccgatcttttccgaatacggcctgatgaaggcgcgcgtcaaagtcgaattaaactggctcaaagccctcgccgccgagccgaagattgccgaagtgccgcccttcagtgccgaaacgcttgccgaaatcgacacggtgattgaaaacttttcattggaagacgcggccgccgtcaaagccatcgaagccaccaccaatcacgatgtcaaagccatcgaatattggctgaaaaaacgttttgccgaagtgccggaagtcgccgccgtgagtgagttcatccacttcgcctgcaccagcgaagacatcaacaacctgtcccacgctttaatgctgcaagaagcgcgtgaggctgttttgctgccgaagctggccgaaatcatcgaaaaactgaccgctatggcgcacgaccttgccgccgtcccgatgatgagccgcacccacggccagcccgccacgccgaccactttgggcaaagaaaccgccaatgtcgtgtaccgcctgcaacgccagtttaaaaacctgcaagcgcaagagttcctcggcaaaatcaacggcgcggtcggcaactacaacgcccatatggtcgcctatcctgatgtagattgggaaacccactgccgcaacttcgtcgaaatcagcctcggtctgaccttcaacccctacaccatccaaatcgaaccgcacgactatatggcggaattcttccaaaccctcagccgcatcaacacgattctcatcgactttaaccgcgacgtttggggttatatttcattgggttacttcaaacaaaaagtcaaagcaggcgaagtcggttcttccaccatgccgcacaaagtcaaccccatcgactttgaaaactccgagggcaacctcggtatggcaaacgccgtattgggctttttgtccgaaaaactgccgatttcccgctggcagcgcgacctgaccgacagcaccgtattgcgcaatatgggcgtaggcgtgggctatgccgtattg

附录Bggtttcgccgcccacctgcgcggtctgaacaagctcgaacccaaccccgccgcgcttgccgccgatttggatgccacttgggagctgctcgccgagccgattcaaaccgtaatgcgccgttacggtgtcgccaatccttacgaaaaactgaaagacctgacgcgcggcaaaggcggcatcacgcccgaagtgctgaaaggctttatcggattgctggaaatccccgccgaagccaaagccaaattgcttgagctgacccccgcgctgtatgtgggcaaggctgaagcgttggcgaaacggatttgagcgtttactgaaaccgatgccgtctgaacgcgcgttcagacggcatttttaagataacgggacatacgggggcgatatttatgcaagctgtccgatacagaccggaaattgacggattgcgggccgtcgccgtgctatccgtcatgattttccacctgaataaccgctggctgcccggaggattcctgggggtggacattttctttgtcatctcaggattcctcattaccggcatcattctttctgaaatacagaacggttctttttctttccgggatttttatacccgcaggattaagcggatttatcctgcctttattgcggccgtgtcgctggcttcggtgattgcctctcaaatcttcctttacgaagatttcaaccaaatgcggaaaaccgtggagctttctgcggttttcttgtccaatatttatctggggtttcagcaggggtatttcgatttgagtgccgacgagaaccccgtactgcatatctggtctttggcagtagaggaacagtattacctcctgtatccccttttgctgatattttgctgcaaaaaaaccaaatcgctacgggtgctgcgtaacatcagcatcatcctgtttttgattttgactgcctcatcgtttttgccaagcgggttttataccgacatcctcaaccaacccaatacttattacctttcgacactgaggtttcccgagctgttggcaggttcgctgctggcggtttacgggcaaacgcaaaacggcagacggcaaacagcaaatggaaaacggcagttgctttcatcactctgcttcggcgcattgcttgcctgcctgttcgtgattgacaaacacaatccgtttatcccgggaatgaccctgctccttccctgcctgctgacggcactgcttatccggagtatgcaatacgggacacttccgacccgcatcctgtcggcaagccccatcgtatttgtcggcaaaatctcttattccctatacctgtaccattggatttttattgctttcgcccattacattacaggcgacaaacagctcggactgcctgccgtatcggcggttgccgcgttgacggccggattttccctgttgagttattatttgattgaacagccgcttagaaaacggaagatgaccttcaaaaaggcatttttctgcctctatctcgccccgtccctgatacttgtcggttacaacctgtacgcaaggggggatattgaaacaggaacacctccgcccgttgcccggcgcgccccttgctgcggaaaatcattttccggaaaccgtcctgaccctcggcgactcgcacgccggacacctgagggggtttctggattatgtcggcagccgggaagggtggaaagccaaaatcctgtccctcgattcggagtgtttggtttgggtagatgagaagctggcagacaacccgttatgtcgaaaataccgggatgaagttgaaaaagccgaagccgttttcattgcccaattctatgatttgaggatgggcggccagcctgtgccgagatttgaagcgcaatccttcctaatacccgggttcccagcccgattcagggaaaccgtcaaaaggatagccgccgtcaaacccgtctatgtttttgcaaacaacacatcaatcagccgttcgcccctgagggaggaaaaattgaaaagatttgccgcaaaccaatatctccgccccattcaggctatgggcgacatcggcaagagcaatcaggcggtctttgatttgattaaagatattcccaatgtgcattgggtggacgcacaaaaatacctgcccaaaaacacggtcgaaatatacggccgctatctttacggcgaccaagaccacctgacctatttcggttcttattatatggggcgggaattccacaaacacgaacgcctgcttaaatcttcccacggcggcgcattgcagtagcctgccttcttgtcggatattgcctttggcagcctatgccgctgtttgccgttcggggcggcggcttttatagtggattaacaaaaatcaggacaaggcgacgaagccgcagacagtacaaatagtacggaaccgattcacttggtgcttcagcaccttagagaatcgttctctttgagctaaggcgaggcaacgccgtactggtttttgttaatccactatattttgccgttttgaggccggggtcggaataaccgttttttgatgattttccctccccggctgtgtcatcaaaaccccaattgcctttccaaactctccaccagattgtcatccagtttcaaagcctgcgacaggcgggcgaggaagacggtttctttccgcgacaaatcggcacagaccaaccttgccgccagataggcctccgccgccaacgcctcatcgttgccgacggcggcggcgatgtcttcgatgcttgcgggaaggcggtattcggcggcgagccatgcggcagtttcggggtctgtgccgctttcctgttcgatagtccggcgttcggcttcgtctatcatgccgtctgaagcggcggcggctatcatggtgcgcaatacggtacggctgtatgtttcttcagtttctccggcaggttggaaatcgctttgtgttacggttgcccgccctttgttttgctgccacatctgatagccccggtaggcgaggtagcccaaagcggcggtcgaaccgattttggtgatggttttgcggtttttaccgttcagcagcatggaggcgacaccggcaaccagcgcgcctccgccgaatgaattgagcgggctgtcggagaatgtgttgccttttttttgaaccgtgcttaagacttggttgagcagtcgggtaaagttcatgaatttttcctttctgttgcgggaagggcggtatgtttaccctatccttttaaacggcggcaggccggccaataattgttggccgtaccgctgtgttttgatgcggttgtcgaggatggttacgcggccgtagtcttgttcggtgcggatgaggcggccgacggcctggatgagtttgatgccggcttcggggacggtgatttcgatgaaggggttgccgccgcgctgttctatccagcggttttgggttttttcgatggggttgtcgggcatggcgaagggaagtttggcgatgatgacttgcacgcaggcggtgccgggcaggtcgagtccttcggcaaagctgtcgagtccgaagatgatgctggctttgccttcttctatggcccggtggtgtttttgcaggaggacggctttgggtaattcgccttgtacgagcaagagcggcaggtagtctccgggcaggcgcagggcgacatcctgcatttgtttgcgcgaggaaaacaagacgagcgtgccgatggcttcggtgggcgaaataagcttgggcagccattcgatgacggcggcggtgtgggcttcggggtctttggggctggcgtatatgggggggatgtagagttcgccctgtttttcaaagtcaaaggggcttttgagggcgagggtggtggtttcgggcagccattgcagcccggtttggcgcagcatcaggttgaagttgcccaaggattgcagggtggcggaagtcaataccgcgcctgccgcacgccgccacaggctgttggcaaggtgggatgcgctgctgatggggctggcgttgaaaatgtagtcgtttttgtcgtcggcgcggcgggttatccatttcgccaacggttcttcaccctcgagggggacagtggagagcaaatcccaaaccgcgctgatttgttcgatacgggcgataaaaagaccgaactcgctggtcaggcggtcgaggagcgcgccgtcctgttctttttcgcggcgtgcggcagaaagcgcatcgttcagcccgataacgtgtttgagcaggctgcgcgcagcaatggccgtattggaaacggtggtttcgaggccttcggggattttgccgtcttcccacagccaagtcggttcgctgttggttcgtctgtcgttttcagacacccccagacttaaagacggctcttccgccaaatggaattgccattcatgcaggctgtcgagcaaggatgcggcggcttcgtcggctaggttggcaagttcggctttatcggtcagcgcggcaattttgccggtcagctgcggcagtttttccagcgtccaaacggcaatattccatgaatgttcggcggcaaaacggctgagggcttttttgggcaggtggtgcgcttcgtcgatgcaatagaaactgttttcgggcgcaggcagaatcacgccgccgcccatactgatgtcggcaagcagaagatcgtggttggcaacgacgacatcgacggtttccaagacatcgcgtgctaggtaaaacggacattccggacggttgggacaggcggttttcaggcagccgtggcggtcgttggtcactttgagccaaatcgcgtcatcgattttttccggccaagtgtcgcggtcgccgttgaaccgtcgggcggaaaattcgtcggcgatgtcgcgcagcagcttcaattcttcgggcttgggtttgctgtcccacaagacggcgggggcttcaaagccgagcaggttttgctgggcattgctttgcgtcagtcgatagagtttgtaggggcagagatagcggccgcgccctttggcaagtgcgaaggtcagttccaaaccgcttttttcgaccagaaacggcaggtcgcggtctaccaactgctcctgcaaggcaaccgtcgcgctgctcacaatcagccgcttgccgcgtgtttgcgccatgatgccgccggccaaaaggtaggccaacgatttgcccacgccggtcggcccttcgatcacggcaatgctctcgccttcgcgcttgggcggctcgccgccttcttcgcgcgccaacgtccgcgaaaaagcgttggcaaccgccgcaatcatttcccgctgcgaagcacgcggacggaaaccgggcaggtttttgccgatgttttggtaatggtcgcggatggcgtttttttctaaatcggtgagcatggcgttttgtacggcggtagaagtgggcttattttaacattgcacggaagcggtacaatatcgttgtcggaatggggggtgaggtgaatcgtgcggacgtggttggttttttggttgcagcgtttgaaatacccgttgttgctttggattgcggatatgttgctgtaccggttgttgggcggcgcggaaatcgaatgcggccgttgccctgtgccgccgatgacggattggcagcattttttgccggcgatgggaacggtgtcggcttgggtggcggtgatttgggcatacctgatgattgaaagtgaaaaaaacggaagatattgagtcattcggacgcaatgccgtctgaaacggaagttcagacggcatttgttttaggttgccgtaccgcttagggaataccggcgacaggatgggcgggatagccgtgggtatcgaccgaacaggcaaaccgccaaggcgtgtggacggtgtcggcggacaggtgggcaagctcgggaatgtgccgtctgacaaaggtgccgtcggggtcggttttgtgtgcggcggcggcaatgtcggggcaggtgtgccgtgaggcggcaagccgccagttgccttggttgattgctgcatcgaaatcggtcagctgtcgggcaaaccatatctcgccttcgcggcgggggaggtttaaaacgtggcagaaaaaatccgcgctcaagcgtctcagggcggggtggaggctgccggttttgtgcaaacagcgcatcgcggcatcgataatcggaatgccggtccggccctgctgccaaagcgtcaggcgcagggtgtgttcaggattgccgtctgaagggtcgtcatccgtgtgctgcaaggcaagttgaaggaaaaaatcgcggcggatgatgttgtccgcccacgcgttcagacggcgttcgaggctttcccgcgcgagcaggcgcggcgagatgcagccggcactcaaatacgcgcccatcagcgaagtgtgtttgcgcgaggggaaatcctttaaaacggagtaggaatccgcctgttcgagaaaccgccgccactgccgccaagccgccgtttcgccgctgttttgcggcaggaagatgccgtctgaaagcgcggcaggctgcggggcggaaaggttttcggggaagggttggcggtatgccgcgaataggtccggaccggcggggggctgcttggaaaagcggtcgagccatacttcgcggtagcggtcgaaatcggcatatgccgtgccgccgtcgggtatcaggtcggttttgccgaaaacggcgcggtcgttgacgaaggttaacgc

附录Bgatgccgtgtttgtccaattcgtgccaaagggcgttgtcggcgagtttgtcggcaaaagtatgggattcgtcggcgatgacggtgcggatattgaggcggacggcgagccggacgagctcggcaggagatgccgccgtgtagagcgggatgccgcgccctgcaagcccttgggcgagttcggcggcggattggcggtagaacgcggcgcggcgagggttgtctgtttcggcatcgtcaatccaaatgccgataatgggcaaacttcggcaacggcggcgcataaggcggcgttgtcgcggatgcggaggttttggcggaaccagacgagcgtgtgtgcggcgcacgtgtccgcataaagggggcgggcggtttcagacggcatttcggcagcctttcctgctggcgattttttcgttcagaaaatcgatgaagctgcggactttcgcgcttaagaatgccctgtctgcataaacggcattcagccggtcggtcgggacggcgtatccgggcagcagcctcaccagcgtgccgcagcgcaaatcgtgttccgccgcccaaagcggctgataaccgatgcacgcgcccgccttaatcatttcgcgcatcatcagcgtgttgtcggtacggatgacgggggtcagttcaagccggtattttttgccgtccgatttgcgggtgaggtcgagtttctgctggttggtgtaggtcggcaggacggcgggcagccccgccacttcttccggcgtttccggcacgccgttgcgcctcaggaaatcgggcgaggcgagcagggcaaattcgatttccgccagtgggcgcgcaatcagcgacggggacagggtttgggaaacgcgcaacgccaaatccacgccttcggcaatcaaatcgacgtggcggttgtccaaaatcagttctaatgccacttcgggataacgttcgcggtattccgccagccagttgcatatctggctgccggcaaaccacagcggcatcgttacgcgcagcagcccctgcggtttttccgtccccccggcggctttttgcgcggcatcgtcgagcgtgtcgagcgcgtaactgcattgccggtagtattcttccccggcttcggtcaggctgaggttgcggctgttgcggtgcaggagtttggcttggacggtgttttccaagtggctgacgtgtttgcttgccattgcggtggagatgccgagcgcgtcggcggcgcgggtgaagccgccgctttggacgacttggcggaaaaccttgaggctgaacagggtgtccatattttcttgtgtggaaaagttgtatcaataaaagcagtatatatttgaaaaggggaaacatctatactctaccgcctgaaatgaagacaaatatcaaaggagcttttatgtccgattgctgcaaccgtatccaaccggttttgctttctgttttgcgtatcgtaaccgcctacctgtttttgttgcacggtacgtcgaaaatcttcgccttccccattgaaatgggcagcggttcgcccggcgggctgttgctgcttgccggtattttagaaattgtcggcggcattttgctggtgttgggcctgtttgcgcgccctgccgcgtttgttttgtccggccagatggcggttgcctattttatggcgcacgcttccggaaatgctttgttcccgattgccaacggcggcgagtccgcagtgctgttctgcttcgtattcctctatatcgcggcggcgggcggcggagcatggtcgctggacaggctgtttttcaagcgtaaagcctgaatcggactgcctaaagtgtattttgttgaatgtttttgaggaaaagaaatgacccgtcaatctctgcaacaggctgccgaaagccgccgttccatttattcgttaaataaaaatctgcccgtcggcaaagatgaagttgtccaaatcgtcgaacacgccgttttgcacacaccttcttcgttcaattcccaatctgcccgcgtggtcgtgctgtttggcgaagagcatgataaggtgtggcaatttgtcgaagacgcgctgcgtgccgtcgtgcctgccgacagttttgaaccgaccgcgcaaaaattgaacctgtttaaggcgggtgcggcaaccattttgttttatgaagatcaaaatgtcgtcaaaggtttgcaggagcagttccctgcttatgccgctaacttccccgtttgggcggatcaggcaaacgcgatggtgcagtatgccgtttggacgacacttgccgcggtcggcgtaggtgcaaacctgcaacattacaatcccttgcccgatgcggcgattgccaaagcgtggaatatccccgaaaactggttgttgcgcgcacaaatggttatcggcggtattgaaggggcggcaggtgaaaagacctttgaacccgttgcagaacgtttgaaagtgttcggcgcataatttcgcggtcaaaaaaatgccgtctgaaccctgttcagacggcatttttcagtatcaggcggcgagttttccgcattctgagacctttgtttacaaatatcatgttcaatatagttaaaagaaattattctcatttcctccgtgaggcaatataattcggttgttttgttaaattgagtataaaaatgaaaatatcatttcatttagctttattacccacgctgattattgcttccttccctgttgctgccgccgatacgcaggacaatggtgaacattacaccgccactctgcccaccgtttccgtggtcggacagtccgacaccagcgtactcaaaggctacatcaactacgacgaagccgccgttacccgcaacggacagctcatcaaagaaacgccgcaaaccatcgatacgctcaatatccagaaaaacaaaaattacggtacgaacgatttgagttccatcctcgaaggcaatgccggcatcgacgctgcctacgatatgcgcggtgaaagcattttcctgcgcggttttcaagccgacgcatccgatatttaccgcgacggcgtgcgcgaaagcggacaagtgcgccgcagtactgccaacatcgagcgcgtggaaatcctgaaaggcccgtcttccgtgctttacggccgcaccaacggcggcggcgtcatcaacatggtcagcaaatacgccaacttcaaacaaagccgcaacatcggagcggtttacggctcatgggcaaaccgcagcctgaatatggacattaacgaagtgctgaacaaaaacgtcgccatccgtctcaccggcgaagtcgggcgcgccaattcgttccgcagcggcatagacagcaaaaatgtcatggtttcgcccagcattaccgtcaaactcgacaacggcttgaagtggacggggcaatacacctacgacaatgtggagcgcacgcccgaccgcagtccgaccaagtccgtgtacgaccgcttcggactgccttaccgcatggggttcgcccaccggaacgattttgtcaaagacaagctgcaagtttggcgttccgaccttgaatacgccttcaacgacaaatggcgtgcccaatggcagctcgcccaccgcacggcggcgcaggattttgatcatttctatgcaggcagcgaaaatggcaacttaatcaaacgtaactacgcctggcagcagaccgacaacaaaaccctgtcgtccaacttaacgctcaacggcgactacaccatcggccgttttgaaaaccacctgaccgtaggcatggattacagccgcgaacaccgcaacccgacattgggtttcagcagcgccttttccgcctccatcaacccctacgaccgcgcaagctggccggcttcgggcagattgcagcctattctgacccaaaaccgccacaaagccgactcctacggcatctttgtgcaaaacatcttctccgccacgcccgatttgaaattcgtcctcggcggccgttacgacaaatacacctttaattccgaaaacaaactcaccggcagcagccgccaatacagcggacactcgttcagccccaacatcggcgcagtgtggaacatcaatcccgtccacacactttacgcctcgtataacaaaggcttcgcgccttatggcggacgcggcggctatttgagcatcgatacgttgtcttccgccgtgttcaacgccgaccccgagtacacccgccaatacgaaaccggcgtgaaaagcagttggctggacgaccgcctcagcactacgttgtctgcctaccaaatcgaacgcttcaatatccgctaccgccccgatccaaaaaacaacccttatatttatgcggttagcggcaaacaccgttcgcgcggcgtggaattgtccgccatcgggcaaatcatccccaaaaaactctatctgcgcggttcgttgggcgtgatgcaggcgaaagtcgttgaagacaaagaaaatcccgaccgagtgggcatccatttgaataataccagcaacgttaccggcaacctgtttttccgttataccccgaccgaaaacctctacggcgaaatcggcgtaaccggtacaggcaaacgctacggttacaactcaagaaataaagaagtgactacgcttccaggctttgcccgagttgatgccatgcttggctggaaccataaaaatgttaacgttacctttgccgcagccaatctgctcaatcaaaaatattggcgttcggactctatgccgggtaatccgcgcggctatactgcccgggtaaattaccgtttctgatgaaatcaggcaaaggctgaaataaaactaaacacattttttcactcaaatcgaacacgccttcaataaaatgccataaaatccgcacattaatctgacacacaagagatacctatgaaactgaaaaccttagctttgacttcattgaccctgttggcattggccgcttgtagcaaacaggctgaaaccagtgttccggcagacagcgcccaaagcagctcatctgctccggcagcccctgctgagttgaacgaaggtgtgaactacactgtattgtctacgcctattccgcaacagcaggccggtaaaatcgaagtattggaatttttcggctacttctgcccgcattgcgcccatcttgagccggtcttgagcgagcacatcaaaacgtttaaagacgatacctatatgcgccgggagcatgtcgtgtggggtgatgaaatgaaacctttggcacgtttggcggccgcagtggaaatggccggtgaatcagataaagccaacagccatattttcgatgcgatggttaatcaaaaaatcaatctggccgataccgataccctgaaaaaatggctgtccgagcaaacagcgtttgacggcaaaaaagtattggctgcatttgaggctcctgaaagccaagcgcgtgcggctcaaatggaagagttgaccaataaattccaaatcagcggcacaccgactgtgattgtcggcggcaaataccaagttgaatttaaagactggcagtccggtatgaccacgattgaccagttggtggataaagtacgcgaagagcagaaaaagccgcaataagttgaggattgaatgagtaaaggccatctgaaaataggatttcagacggccttttgtatttaggctttatagaagagatgattgcttaaagccttatggttttaaatcagaatatatagcggattaacaaaaaccagtacggcgttggctcgccttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttccgtactatctgtactgtctgcggctcgccgccttgtcctgatttttgttaatccactataaatcagaatataaaacaaaaacgccgtctgaaatttcagacggcgttttctgttaaatcggcttacaaacccgggaacatcccttttatccccctcattcctttcgccatacgcatcagtttgcccaagccgttgccgctgaacatcttcatcatttgttgcatttgttcaaactgtttgagcaatttgttcacttcctgcacggttgtgcccgcacccattgcaatacggcgtttgcggctggctttgagcagggcagggttggcgcgttctttaggggtcatcgagttgatgatggcttctactttgcccatcgctttttcagccgttccttcggggatttgtttcgagatttgacccagttcgcccggcattttcgacatcaggttttccaaaccgcccatattgcgcatttgctggatttgttctttaaagtcgttgaggtcgaagcctttgcctttgtgcagctttttcgccattttagcggcggcttcttcgtctatacctttttgaacgtcttcaatcagggtcaatacgtcgcccatacccaaaatgcggccggcaagacggtcggggtggaaaggttcgaggccgttgattttttcgccgacaccgataaatttaatcggtttgccggttacgtggcgtacggacaatgccgcaccgccgcgcgagtcgccgtccatcttggtcaatacgactccggtcagcggcagggcttcattaaatgcctgagcagtgttcaccgcatcctgacccagcatcgcatcgatgacgaacaaagtttccaccgggttaaccgccgcgtgaagggctttgatttcgttcatcatctcttcat

附录Bcgattgccaaacggccggcggtatcgaccatcaatacatcgtaaaaatgttttttggcgtaatcgacggcggcagttgcaatttcaaccggtttttggttggtatcggacgggaaaaaatccacgccgacctgttcggccaacagacgcagctgttcaatcgcggcaggacggtaaacgtcggcggataccaccaaaacctttttcttctgatcgtttttcaacaggcgggcgagtttgccgacggtcgtcgtcttgcctgcgccctgcaaacctgccatcaacacgacggcgggcggcgcaaccgacaaatccagcgttttgttttccctgcccatcagttcggtcagggctttgttgaccacgccgataaatgcctgatccggcgtcaggctgcccgctacttcctgaccgagggccttttctttgacgttgttgatgaactctttgacgacaggcagggcgacatccgcctcaagcagggcgaggcggacttcgcgcaaggcctctttaatattgtcttcggtcagtttggcctgcccccggatgtttttgaagacattgctgaagcggccggttaaattgtctaacatactggtccttggtctgaataagaatagcttgccccatcaggggcattctttgttaaaataaaatcaaaataatttgatgcggcttgtgtgccggacagcatatcggcaaatccgtcaaggcttgaccgaaatggggattttacaattccaacgttaaaagttccaatatttcataagcggccgcatacggcgcaacagtatagatagagaaagtccaccatgccgacagttttcatctttttgacggcggtttacgcaggattgggtgcatttgcatggcactgccaacagcaggggtgcggccgggattacccgtggaagacggaattgccggttttgggtgcggcattgaccgtccacggcgcggcactgcttatgccggtcattcaagacaaaatcatcattatgggcttcgggtattccggcagcctgattgtttggatgatgctgtttatttattttgccggcagcttcttttatccgctgcgcggagtgcagttgctgctgtatccttgcgccgcactgatgctgctgtcaggtttggtttttcctggaaaattctcgggatatgaaattaccgaccttccctttatgctgcatatcggaacttcgctgctcgcatacgggctgttcggcatcgcaacattattgtccgttttgaccctgctgctgaatcggagcctgcaccgcaggagcttctccaagctcgcaggattcctgccgtcgctgctcagtttggaaaaactcatgttccaggccatgtgggcaggtttcatcctgctgacctattccgtcgtcagtggaacattttttgccgaagccgtattcggcaaacccatgacctttacccataaaaccgtattcggcatattgtcatggctgatttacggcggactgctgctcaagcacagcatgaccgcatggcgcggcaaaaaagccgccgtgtggaccatcatcggatttgtcagccttatgattgcctatatgggcagcaagttcgtattggaaatcattctgaaaagataagaagagccaacagatgccgtctgagtccccgagtttcagacagcatattcacaaaggcgcaccagccggaggagggagaggaaaggattgttggaggcggcgcagtatttagcagaaataaaaaaccttatccgacagcgacatgacgaatttccccaaaaaaatcccgctgaaagcattgaccgtttttccctgtgggcgtatagttcggttcttcgctgctgcagaagtggcggacgaactgaaaagtatagcacagaatgttggggatatcgagagatatcttgacaggcggaaggaatactttataattcgcaacgctctttaacaaaacagattaccgataagtgtgagtgccttgagtctcacactgtttgaaagacagacaagataatgttttgaacattgtcctgttggtttctttgaagcagaccagaagttaaaaagttagagattgaacataagagtttgatcctggctcagattgaacgctggcggcatgctttacacatgcaagtcggacggcagcacagagaagcttgcttctcgggtggcgagtggcgaacgggtgagtaacatatcggaacgtaccgagtagtgggggataactgatcgaaagatcagctaataccgcatacgtcttgagagagaaagcaggggaccttcgggccttgcgctattcgagcggccgatatctgattagctagttggtggggtaaaggcctaccaaggcgacgatcagtagcgggtctgagaggatgatccgccacactgggactgagacacggcccagactcctacgggaggcagcagtggggaattttggacaatgggcgcaagcctgatccagccatgccgcgtgtctgaagaaggccttcgggttgtaaaggacttttgtcagggaagaaaaggctgttgctaatatcagcggctgatgacggtacctgaagaataagcaccggctaactacgtgccagcagccgcggtaatacgtagggtgcgagcgttaatcggaattactgggcgtaaagcgggcgcagacggttacttaagcaggatgtgaaatccccgggctcaacccgggaactgcgttctgaactgggtgactcgagtgtgtcagagggaggtagaattccacgtgtagcagtgaaatgcgtagagatgtggaggaataccgatggcgaaggcagcctcctgggacaacactgacgttcatgcccgaaagcgtgggtagcaaacaggattagataccctggtagtccacgccctaaacgatgtcaattagctgttgggcaacctgattgcttggtagcgtagctaacgcgtgaaattgaccgcctggggagtacggtcgcaagattaaaactcaaaggaattgacggggacccgcacaagcggtggatgatgtggattaattcgatgcaacgcgaagaaccttacctggtcttgacatgtacggaatcctccggagacggaggagtgccttcgggagccgtaacacaggtgctgcatggctgtcgtcagctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccttgtcattagttgccatcattcagttgggcactctaatgagactgccggtgacaagccggaggaaggtggggatgacgtcaagtcctcatggcccttatgaccagggcttcacacgtcatacaatggtcggtacagagggtagccaagccgcgaggcggagccaatctcacaaaaccgatcgtagtccggattgcactctgcaactcgagtgcatgaagtcggaatcgctagtaatcgcaggtcagcatactgcggtgaatacgttcccgggtcttgtacacaccgcccgtcacaccatgggagtgggggataccagaagtaggtaggataaccacaaggagtccgcttaccacggtatgcttcatgactggggtgaagtcgtaacaaggtagccgtaggggaacctgcggctggatcacctcctttctagagaaagaagaggctttaggcattcacacttatcggtaaactgaaaaagatgcggaagaagcttgagtgaaggcaagattcgcttaagaagagaatccgggtttgtagctcagctggttagagcacacgcttgataagcgtggggtcggaggttcaagtcctcccagacccaccaagaacgggggcatagctcagttggtagagcacctgctttgcaagcagggggtcatcggttcgatcccgtttgcctccaccaatactgtacaaatcaaaacggaagaatggaacagaatccattcagggcgacgtcacacttgaccaagaacaaaatgctgatataataatcagctcgttttgatttgcacagtagatagcaatatcgaacgcatcgatctttaacaaattggaaagccgaaatcaacaaacaaagacaaagcgtttgttttgattttttattctttgcaaaggataaaaatctctcgcaagagaaaagaaaacaaacacagtatttgggtgatgattgtatcgacttaatcctgaaacacaaaaggcaggattaagacacaacaaagcagtaagctttatcaaagtaggaaattcaagtctgatgttctagtcaacggaatgttaggcaaagtcaaagaagttcttgaaatgatagagtcaagtgaataagtgcatcaggtggatgccttggcgatgataggcgacgaaggacgtgtaagcctgcgaaaagcgcgggggagctggcaataaagcaatgatcccgcgatgtccgaatggggaaacccactgcattctgtgcagtatcctaagttgaatacatagacttagagaagcgaacccggagaactgaaccatctaagtacccggaggaaaagaaatcaaccgagattccgcaagtagtggcgagcgaacgcggaggagcctgtacgtaataactgtcgagatagaagaacaagctgggaagcttgaccatagtgggtgacagtcccgtattcgaaatctcaacagcggtactaagcgtacgaaaagtagggcggggcacgtgaaatcctgtctgaatatggggggaccatcctccaaggctaaatactcatcatcgaccgatagtgaaccagtaccgtgagggaaaggcgaaaagaaccccgggaggggagtgaaacagaacctgaaacctgatgcatacaaacagtgggagcgccctagtggtgtgactgcgtaccttttgtataatgggtcaacgacttacattcagtagcgagcttaaccgaataggggaggcgtagggaaaccgagtcttaatagggcgatgagttgctgggtgtagacccgaaaccgagtgatctatccatggccaggttgaaggtgccgtaacaggtactggaggaccgaacccacgcatgttgcaaaatgcggggatgagctgtggataggggtgaaaggctaaacaaactcggagatagctggttctccccgaaaactatttaggtagtgcctcgagcaagacactgatgggggtaaagcactgttatggctagggggttattgcaacttaccaacccatggcaaactaagaataccatcaagtggttcctcgggagacagacagcgggtgctaacgtccgttgtcaagagggaaacaacccagaccgccagctaaggtcccaaatgatagattaagtggtaaacgaagtgggaaggcccagacagccaggatgttggcttagaagcagccatcatttaaagaaagcgtaatagctcactggtcgagtcgtcctgcgcggaagatgtaacggggctcaaatctataaccgaagctgcggatgccggtttaccggcatggtaggggagcgttctgtaggctgatgaaggtgcattgtaaagtgtgctggaggtatcagaagtgcgaatgttgacatgagtagcgataaagcgggtgaaaagcccgctcgccgaaagcccaaggtttcctgcgcaacgttcatcggcgtagggtgagtcggcccctaaggcgaggcagaaatgcgtagtcgatgggaaacaggttaatattcctgtacttgattcaaatgcgatgtggggacggagaaggttaggttggcaagctgttggaatagcttgtttaagccggtaggtggaagacttaggcaaatccgggtcttcttaacaccgagaagtgacgacgagtgtctacggacacgaagcaaccgataccacgcttccaggaaaagccactaagcttcagtttgaatcgaaccgtaccgcaaaccgacacaggtgggcaggatgagaattctaaggcgcttgagagaactcaggagaaggaactcggcaaattgataccgtaacttcgggagaaggtatgccctctaaggttaaggacttgctccgtaagccccggagggtcgcagagaataggtggctgcgactgtttattaaaaacacagcactctgctaacacgaaagtggacgtatagggtgtgacgcctgcccggtgctggaaggttaattgaagatgtgagagcatcggatcgaagccccagtaaacggcggccgtaactataacggtcctaaggtagcgaaattccttgtcgggtaagttccgacccgcacgaatggcgtaacgatggccacactgtctcctcctgagactcagcgaagttgaagtggttgtgaagatgcaatctacccgctgctagacggaaagaccccgtgaacctttactgtagctttgcattggactttgaagtcacttgtgtaggataggtgggaggcttagaagcagagacgccagtctctgtggagccgtccttgaaataccaccctggtgtctttgaggttctaacccagacccgtcatccgggtcggggaccgtgcatggtaggcagtttgactggggcggtctcctcccaaagcgtaacggaggagttcgaaggttacctaggtccggtcggaaatcggactgatagtgcaatggcaaaaggtagcttaactgcgagaccgacaagtcgagcaggtgcgaaagcaggacatagtgat

附录Bccggtggttctgtatggaagggccatcgctcaacggataaaaggtactccggggataacaggctgattccgcccaagagttcatatcgacggcggagtttggcacctcgatgtcggctcatcacatcctggggctgtagtcggtcccaagggtatggctgttcgccatttaaagtggtacgtgagctgggtttaaaacgtcgtgagacagtttggtccctatctgcagtgggcgttggaagtttgacgggggctgctcctagtacgagaggaccggagtggacgaacctctggtgtaccggttgtaacgccagttgcatagccgggtagctaagttcggaagagataagcgctgaaagcatctaagcgcgaaactcgcctgaagatgagacttcccttgcggtttaaccgcactaaagagtcgttcgagaccaggacgttgataggtggggtgtggaagcgcggtaacgcgtgaagctaacccatactaattgctcgtgaggcttgactctatcatttgaagaacttcaagagataaaagcttactgactgattcagtcattaccgaatatattgattaaggctttaccgatttgtaacagtttaagtttggcggccatagcgagttggtcccacgccttcccatcccgaacaggaccgtgaaacgactcagcgccgatgatagtgtggttcttccatgcgaaagtaggtcactgccaaacacccattcagaaaacccccgattattcgggggtttttgctttgcccggaaaaaatgtttgctttgcccggaaaaaatgtcggtgatggcgggacggcatccgtacggtgtccggtcgggtttgcggaggaacggcttgaaactttgggatattcattttagaatgactcgttttatcgtcgcaagatgcggtttattgtttgcaacccttaaaggaaaaaccatgaagaaaatgttcgtgctgttctgtatgctgttctcctgcgccttctcccttgcggcggtaaacatcaatgcggcttcgcagcaggagttggaggcgctgccgggcataggcccggcgaaggcgaaggccattgcggaataccgtgcgcaaaacggtgcgttcaagtctgtagacgatttgaccaaggtaaagggcatcggccctgcggtgctggcgaagctgaaggaccaggcttcggtcggcgcgcccgcaccaaaagccccagccaaaccggtgctgcccgcggataaaaaataggggaacctgtaaaggaaagggcatcggccgccgtcggtgcttttttgtttggaagggaaatggctaaaatatgtagcattatgttctgtatcgttgtttaccgcttccgcacctttgtccgccttaaagcaggtagacaccgcaatgaatcgacgcaaagaaaatgccgtctgaacatgcgttcgggcggcgttttgttggggggtatcggagcggaacgtctgaaaaagggtttcaggcggtctttgggcgtgtggtgacagtcgaaaacgtgataaggctacctgaaaagtttgggagattttcaggtagcctttggtattgggcgcaacagacgcaggtacagattagcggtgtgccgtaatcgtacgaatgccgattcaacctaagcagacatcagtatttaggaagtggatgtttgatggagcaaaggttgtacgaagggtggaaggcaacctgtgggtgtttggtatggtcgcgcttgaaaaaacgtgttttaagggacaaatgccgtctgaaaatcggtttcagacggcattttctgtttatttaaagcaaacaggaaaaggcagcaatattctgcagtcttcctattcacacaagcgttttatagttaattaaaaacaaaatagtacaatactcaactttgaaggtctaaccatggcatactctgcggacttaagaaacaaagctttaaactaggggctgtactagattagcagatatgttaccctcgaaatatgaagataacgcactgcaaattaaagaaaaaagtacagaaagaactgctccgttttttgtgctggaagttaccgcccgttctgccgccgatattttgggtatccatcccaattcggcagtactgttctaccgtaaaatccgcacggttatcaaccatcatttggccttggctgccgatgaggtttttgagggccctgtcgagccggacgaaagcgatttcggcggacggcgtaaaggcagacgtggtcgcggtgcggcaggaaaagtggttgtcttcggcattctgaaacgcaacggacggggctataccgttgtcgtagataatgccaagtctgaaacgttactccctgtcatcaaaaagaaaatcatgccggacagtattgtttataccgatagtctgagcagctgcgacaagttggacgtgagcggttttatccattaccgcatcaaccattccaaggaatttgcagaccgtcagaaccacattaacggcattgagaatttttggaatcaggcaaaacgcgtcttgcgaaaatacaacggaatcgatcgtaaatctttcccgctgttcttgaaagaatgcgaatttcgatttaacttcggcacaccgtctcaacagcttaaaatcctgcgggattggtgtggaatttagggctaatctagtacagcacctaacaaaaaccagtacggcgttggctcgccttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttccgtactatttgtactgtctgcggcttcgtcgccttgtcctgatttttgttaatccactatattttagataatgcgtgatttcaccgtatgggtgtcttacgggaaatggcggaaaaattgggacataaggtattgcctcttgcaccttattcacctgagctcaacccgattgagaaagtgtgggcgaatattaagcggtatctgcgaaccgttttgtctgattacgcccgatttgacgatgcactactgtcctattttgattttaattgactatagaacgttgcggctacgcggaagccgtactcgttggatttggagcggcccattttggttttgtcaccgtccaagacaatctcacggggtttgtagattgttttgtgacggtagtatggatcaaactcgagaccgacgctgtcagtcaactgtttgcctacattcagaccgataccgacactccaacctttggcgcttttgctgacatcgcgggaagcacccatctgggtcgtcatcactttggttttgccgcgcaaatctgcatatgcatccgcccaaggggtcagggatcatccgtcccccaaatcttggcggatttcgccatggactttcaaagcaaggttttcatgcttggtaacggtgtttttccttatcgccgatgatggctttgcctttgccgttagactcgggaatatcggctaccgtaacggcggacacggctgcaagtgagagtgcaagcagggttttttcatgtttttcttcctataatgaggataaataaatggaaaaagtgtgggaaatacccgcattcccattaaatcttttttcaagcaatgagttctttttgttttcaacattttccttgagacctttgcaaaaatagtctgttaacgaaatttgacgcataaaaatgcgccaaaaaattttcaattgcctaaaaccttcctaatattgagcaaaaagtaggaaaaatcagaaaagttttgcattttgaaaatgagattgagcataaaattttagtaacctatgttattgcaaaggtctctccttgtgtatgaaattttgccggatgtgaaggcggaatcggcagcgggggtgttctgtaccggattgtcgtggaaatgggaaaacggatgttccgtgcaggtttgtccaaatgaatggcgggtattgtttttatcaatctgtttctttttatttgaaataaaatttctaaaataataaaaatatgaaatttaaaatctataaaaaaagatatatcagttattttgaaataaaatagctttgtagtaatatgttgcacttgtttgtgcaaggtaaacgatgtaacctaagccgcgtataaaaacccatcaggaaagatgcaagatgacacaccattaccccacagacgatattaagattaaagaagttaaagagttgttgccgccgatagcccatctttacgagctgccgatttccaaagaggcttcgggcttggttcaccgcacccgtcaggaaatttccgatttggttcacggcagggacaagcggctgttggttattatcgggccgtgttcgattcacgatccgaaagcggcgttggaatatgcggagcgtttgttgaaactccgcaagcagtatgaaaacgagcttttgattgtgatgcgcgtttatttcgagaagccgaggacgacggtgggttggaaaggtttgattaacgacccgcatttggacggtacgtttgacatcaatttcggtttgcgtcaggcgcgcagcctgttgttgtcgctgaacaatatgggtatgcctgcctctaccgagtttttggatatgattacgccgcaatattatgcggacttgatttcttggggggcaatcggtgcgcggacgaccgaaagccaagttcaccgcgaattggcaagcgggctgtcctgccccgtcggctttaaaaacggtacggacggcaatttgaagattgccatcgacgcaatcggtgcggcgagccattcgcatcatttcctgtctgtaaccaaggccgggcattccgccattgtccataccggcggcaatcccgactgtcatgtcattttgcgcggcggcaaagagccgaattatgatgcggaacacgtcagcgaggcggcggaacaactgcgtgcggcaggggtaaccgacaagctgatgatagattgcagccacgccaacagccgcaaggattacactcggcagatggaagtggcacaagacattgccgcccaattggaacaggacggcggcaatatcatgggcgtgatggtggaaagccatttggtcgaaggcagacaggacaagccggaagtgtacggcaagagcattaccgatgcgtgtatcggttggggcgcgactgaagaactgttggcattgttggcaggtgcaaacaaaaaacgtatggcgcgcgccagttgagatttttgacgcagaatgtcataaaatgtcgtctgaagcgttcagacggcatttttgtggaggaaatatgctcaaaataaccctaattgcggcgtgtgcggaaaacctgtgcatcggggcgggcaatgctatgccttggcacatccccgaagatttcgcatttttcaaagcctataccttgggcaaacccgtcattatggggcggaaaacgtgggaatccctgcccgtcaaacccctgcccggacggaggaacatcgtcatcagccggcaggcggattattgcgcggcaggcgcggaaacggcggcaagtttggaggcggcattggcattgtgcgcaggcgcggaagaagccgtcattatgggcggcgcgcagatatacggacaagcgatgccattggcgaccgatttgcggataaccgaagtggatttgtctgtggaaggagatgcatttttccccgcaatagaccggacgcattggaaagaagcagagcggacggaacgccgtgtcagcagcaaaggcacgcgctatgcttttgtgcattatttgagatattgaaatataaactctctataaaatcccccgcaaatgatgggctgaaatagaaaatattgttattcccccgaagatgggaatccgggattttaaagttagggtaatttatccgaaataacaacaatcttccatcgtcattcccgcaaaagcgggaatccggaaacgaaaagctaaagcaatttatcggaaaaaaccgaagtttaaagaaccggattcccgcctgcgcgggaatgacgagattttaggttatggggatttattgggaataatggaacaaagaaagcagaaataaggatatagaggctgtctttggatttgcgatggttgtcggagaatgccgtctgaagccgtttcagacggcatttttccagcttgagaacggatgcctgctcaaataagcattggtaaacataccgtcggcagtgatttcccgtcccagccagtccggacggtcaaaatcggcattctcgtcgggcaactcgatttccgcgacgaccaaaggcgcattatcgccaagaaaaacatcgatttcaaacaggctgccgccccatctgaccggataacgccatttttccattttaaacgggcacatcgtttccatcatcttttccgcatcggcaagcgggatttcgtattcaaactcactgcggctgatttccgaaatatagcctttcagcgtcagccacgcctgttttccggcaatgcggacacggacggtgcgttctttttcaacagacagataaccctgcctcaacagcagcggttcgtcggcgtattgccgccagttgtcgtttccaatcaaaaaacggcgttcgatttctatcggcataagatgctccgtcaaaacggtttgaacacgaccagatacagcgcggcaaccatcagcagcacggggatttcgttgaacacgcggtaccagcggtgtgaaaaagcattgctgtaatcctga

附录Baaacggcgcagcagcacgccgcaatacaactggtaagccaagagcatcaagcccaaacacagtttgacgtgtacccagccgctgccccaccagccggcggcaaacggtatcgccgcgccgaacacgaccgcgccgaagcccaacggcgacataaaacggtacagccgcaccgccatgcccgacagacgcacatactcgggattgccgcgcggcacatcaatcatcgccatattgacgaaaatcctcggcaggtaaaacagccctgcaaaccacgaaatgacaaaaaacaagtgaaacagcttgaaccaagaaaacatcatcgcccacaccctgccgaaaagcggtattgtacaggcaaaccgcttgggaaacgtgataaaatcaggcggataaacaaatcgaataaatccttaccgcaaaacggaggcaaaatgctcaaatccatcgaactcaattcccacatccgcaaccgccttgcagaatatctgaaaggcaggggtatggattttcagacggcaatgcaggaagaaaaaggcaacaaagaaatcgccgccatcgtccacagcggtttgcccactctggtccgcaaactgtattccgaacaaaaaatgcagaagtttttttgggaaaagcgggatttgattgccgactacatcagccgccggatgcagggataggtggctgaaatctgttttcaggcaagtgaaaagacaatatggcagattgaaattacgcttatcgtcattcccgcccgcgcgggaatccgacttgtttggtttcggttatttttcgtttcgtaacttttgagccgtcattcccgcgcaggcggtaatccggcttgttcggtttcggttctttttctcgtttcgggtgatttctaaaccgtcattcccgcgcaggcgggaatctaggtctttaaacttcggttttttccgataaatttttgccgcattaaaattctagattcccgctttcgcgggaatgacggcggagggtttttagttttcccgaaaatgcacatcatccaaaatcccgttattcccacaaaacagaaaatcaaaaacagcaacctgaaatcccgtctttcccgcgcaggcggtaatctgaacacgtccgtagtgaaacctatatcccgtcattcgcacgaaagtgggaatccaggatgcagggaaaaccgttttatccgataagtttccgcaccgaaaggtctagattcccgctttcgcgggaatgacggcggagggtttttagttttctcgataaatgcacatcatccaaagtcccgttattcccacaaaaacagaaaatcaaaaacaacaatctgaaattccgtccttcccgcctgtgcgggaatccggcttgttcggtttcggttctttttctcgtttcgggtgatttctaaaccgtcattcccgcgcaggcgggaatctaggtctttaagcttcggtttttcttgataaattcttgccgcattaaaattctagattcccgctttcgcgggaatgacggcggagggttttttgttttcccgataaatgcacatcatccaaagtcccgttattcccacaaaaacagaaaatcaaaaacagcaacctgaaatcccgtccttcccgcgcaggcggtaatctgaacacgtccgtagtgaaacctatatcccgtcattcgcacgaaagtgggaatccaggatgcagggaaaaccgttttatccgataagtttccgcaccgaaaggtctagattcccgctttcgcgggaatgacggcggagggtttttagttttctcgataaatgcacatcatccaaaatcccgttatttccacaaaacagaaaatcaaaaacagtaacctgaaatcccgtcattcccgcgcaggcgggaatccggcttgttcggtttcggttctttttcttgtttcgggtgatttctaaaccgtcattcccgcgcaggcgggaatccagacctttaaaccccgaccatccttgataaattcttgcggcattaaaattctagattcccgctttcgcgggaatgacggcggagggttttttgcttttcctgatttttcattgcgatgtagtataatgtagtatataatcattataattttaacacttgacaaaggaaaatttctcatgacactgaaagcaagcaagcaagcaagcaagcaagcaagcaagcggtcgggttaatctattaacattatctgttttatcgctgttttgcacgccatatgtttgaggttcggatgcgtacgatcccgtcaaagaagccgagattaaaaacaaatttattttagaagcggcggaagacagaaattcccacgtttggcgcggcccgtgcagcatatcttttgattgcttcggtatgttcagagctcagcttggttcaaatactcgttctaccaaaatcggcgacgatgccgatttttcattttcagacaagccgaaacccggcacttcccattatttttccagcggtaaaaccgatcaaaattcatccgaatatgggtatgacgaaatcaatatccaaggtaaaaattacaatagcggcatcctcgccgtcgataatatgcccgttgtcaaaaaatatattacagagaagtatggggctgatttaaagcaggcggttaaaagtcaattacaggatttatacaaaacaagaccggaagcttgggcagaaaataaaaaacggactgaggaggcgtatatagcacagtttggaacaaaatttagtacgctcaaacagacgatgcccgatttaattaataaattggtagaagattccgtactcactcctcatagtaatacatcacagactagtctcaacaacatcttcaataaaaaattacacgtcaaaatcgaaaacaaatcccacgtcgccggacaggtgttggaactgaccaagatgacgctgaaagattccctttgggaaccgcgccgccattccgacatccatacgctggaaacttccgataatgcccgcatccgcctgaacacgaaagatgaaaaactgaccgtccataaggattatgcgggcggcgcggatttcctgttcggctacgacgtgcgggagtcggacgaacccgccctgacctttgaagacaaagtcagcggacaatccggcgtggttttggaacgccggccggaaaatctgaaaacgctcgacgggcgcaaactgattgcggcaaaaacggcggattccggttcgtttgcgtttaaacaaaattaccggcagggactgtacgaattattgctcaagcaatgcgaaggcggattttgcttgggcgtgcagcgtttggctatccccgaggcggaagcggttttatatgcccaacaggcttatgcggcaaatactttgtttgggctgcgtgccgccgacaggggcgacgacgtgtatgccgccgatccgtcccgtcaaaaattgtggctgcgcttcatcggcggccggtcgcatcaaaatatacggggcggcgcggctgcggacgggtggcgcaaaggcgtgcaaatcggcggcgaggtgtttgtacggcaaaatgaaggcagccgactggcaatcggcgtgatgggcggcagggccggccagcacgcatcagtcaacggcaaaggcggtgcggcaggcagtgatttgtatggttatggcgggggtgtttatgctgcgtggcatcagttgcgcgataaacaaacgggtgcgtatttggacggctggttgcaataccaacgtttcaaacaccgcatcaatgatgaaaaccgtgcggaacgctacaaaaccaaaggttggacggcttctgtcgaaggcggctacaacgcgcttgtggcggaaggcattgtcggaaaaggcaataatgtgcggttttacctacaaccgcaggcgcagtttacctacttgggcgtaaacggcggctttaccgacagcgaggggacggcggtcggactgctcggcagcggtcagtggcaaagccgcgccggcattcgggcaaaaacccgttttgctttgcgtaacggtgtcaatcttcagccttttgccgcttttaatgttttgcacaggtcaaaatctttcggcgtggaaatggacggcgaaaaacagacgctggcaggcaggacggcactcgaagggcggttcggtattgaagccggttggaaaggccatatgtccgcacgcatcggatatggcaaaaggacggacggcgacaaagaagccgcattgtcgctcaaatggctgttttgatgcgtcgggaaatgttttgacgcacaggcggtacaccggcacggcaccgcgcgccgccccgcaaaccaatccgaaccctgccgccccgaagggcggggcataatgatgaaaccggcggaaaaccgccggttttttgccgccgtttgaaacccgattctggcttcagacggcattgtcgcggcatcgggcggcagggtttggaacagcggcataaaaaactgatacaatccgccgattgataatggttattttttatttttgtgggaagacatttatgcctgcacgaaacagatggatgctgctgctgcctttattggcaagcgcggcatatgccgaagaaacaccgcgcgaaccggatttgagaagccgtcccgagttcaggcttcatgaagcggaggtcaaaccgatcgacagggagaaggtgccggggcaggtgcgggaaaaaggaaaagttttgcagattgacggcgaaaccctgctgaaaaatcccgaattgttgtcccgcgcgatgtattccgcagtggtctcaaacaatattgccggtatccgcgttattttgccgatttacctacaacaggcgcagcaggataagatgttggcactttatgcacaagggattttggcgcaggcagacggtagggtgaaggaggcgatttcccattaccgggaattgattgccgcccaacccgacgcgcccgccgtccgtatgcgtttggcggcagcattgtttgaaaacaggcagaacgaggcggcggcagaccagttcgaccgcctgaaggcggaaaacctgccgccgcagctgatggagcaggtcgagctgtaccgcaaggcattgcgcgaacgcgatgcgtggaaggtaaatggcggcttcagcgtcacccgcgaacacaatatcaaccaagccccgaaacggcagcagtacggcaaatggactttcccgaaacaggtggacggcacggcggtcaattaccggctcggcgcggagaaaaaatggtcgctgaaaaacggctggtacacgacggcgggcggcgacgtgtccggcagggtttatccggggaataagaaattcaacgatatgacggcaggcgtttccggcggcatcggttttgccgaccggcgcaaagatgccgggctggcagtgttccacgaacgccgcacctacggcaacgacgcttattcttacaccaacggcgcacgcctttatttcaaccgttggcaaaccccgaaatggcaaacgttgtcttcggcggagtgggggcgtttgaagaatacgcgccgggcgcgttccgacaatacccatttgcaaatttccaattcgctggtgttttaccggaatgcgcgccaatattggatgggcggtttggatttttaccgcgagcgcaaccccgccgaccggggcgacaatttcaaccgttacggcctgcgctttgcctgggggcaggaatggggcggcagcggcctgtcttcgctgttgcgcctcggcgcggcgaaacggcattatgaaaaacccggctttttcagcggttttaaaggggaaaggcgcagggataaagaattgaacacatccttgagcctttggcaccgggcattgcatttcaaaggcatcacgccgcgcctgacgttgtcgcaccgcgaaacgcggagtaacgatgtgttcaacgaatacgagaaaaatcgggcgtttgtcgagtttaataaaacgttctgattgctgttccttttcggaggaaaccctgccggcggcggtatcacggcgggcatcggcggctttcgggcggtgctttgcgtgccgccgcgtgtgcggaaacgcattccggtttttccggcataacggcgatgcgaggtaaaatgccgtctgaaacccgattcgggcttcagacggcattgtcgcggttgcggcgggcgggttcaccagattccgtcaaaggttttcgcgccgcgccaaaatttccacctgtcggcgggtttgaaggtcagcgtaccgccgtgttgtccgtccgtggtgatgtccagccgtttgattttgccggtgcggacggcttcgtagattggtgcgaaccagcgttcttcccactgctgcaatattgccgcataccgctccctgtcccctgtcagggcggtcaggcgcaaatcgtccataaacaggatatggtgcgtgtcgggcaggtgtgccgccgtttcttcataggcgcggaagttgtcgggtaatgcgcggcggtcggagtggaaacggctccaaaccgtatcggcgaaaagcgtgccgccttgcgcgccgccgtttgtgccgtcccaaagccataagccgttcaactcgggcagcccgcgtttcttgcggttatggttgacggggtgcgccgccagccacatttggatttcggtttggacgcgcagccattccaacgcatcttctccgtccggctgatcgtcagcgcccaacaat

附录Bccgcccaagtccaaaacgggcttcgcgccccagcggtacgcgcaaggaagggaaaccagccataattcgggcaggacgggaacgaaacgccatggaatgtcgccgtaaaacgccgacaggtcgcggcagatccgttccgcttcatccgtaccgacgttcagatattccgccgttagcacatttgcctgatgcatccccatcttttgccagacgggcgtggcgagcgcgacggcttcagacggcatattcaggctttgcgccgcgcgttccgccagtctgccgcaccacaaataacgcgcgtaaaatgccgaagccgtgcagctttggcggtgcagcgagccgtattgcaggattttgttgaaagcgtgcaggcatagaggtattcggatttcgtcttcatccaaattgagcgagggaatggcgagggtgagtttcatcgtttgacgtttcagaaatgcaggtcaggcgcaacattatagaggattcggcgcaaacgccgtcaaaaaggaacaatatggctgtcttcccactttcggcaaaacatcggaaatacgcgctgcgtgcgcttgccgtttcgattattttggtgtcggcggcatacattgcttcgacagagaggacggagcgcgtcagaccgcagcgcgtggaacaaaatctgccgccgctgtcttggggcggcagcggcgttcagacggcatattgggtgcaggaggcggtgcagccgggcgactcgctggcggacgtgctggcgcgttcgggtatggcgcgggacgagattgcccgaatcacggaaaaatatggcggcgaagccgatttgcggcatttgcgtgccgaccagtcggttcatgttttggtcggcggcgacggcggcgcgcgcgaagtgcagttttttaccgacgaagacggcgagcgcaatctggtcgctttggaaaagaaaggcggcatatggcggcggtcggcttctgaggcggatatgaaggttttgccgacgctgcgttcggtcgtggtcaaaacgtcggcgcgcggttcgctggcgcgggcggaagtgcccgtcgaaatccgcgaatccttaagcgggattttcgccggccgcttcagccttgacggtttgaaggaaggcgatgccgtgcgcctgatgtacgacagcctgtatttccacgggcagcaggtggcggcgggcgatattttggcggctgaagtcgttaagggcggcacaaggcatcaggcgttctattaccgttcggacaaggaaggcggagggggcggcaattattatgatgaagacggcaaggtgttgcaggaaaaaggcggcttcaacatcgagccgctggtctatacgcgcatttcttcgccgttcggctaccgtatgcaccccatcctgcacacatggcggctgcacacgggcatcgattatgccgcaccgcagggaacgccggtcagggcttccgccgacggcgtgattacctttaaaggccggaagggcggatacggcaacgcggtgatgatacgccacgccaacggtgtggaaacgctgtacgcgcacttgagcgcgttttcgcaggcggaaggcaatgtgcgcggcggcgaggtcatcggttttgtcggttcgaccgggcgttcgaccgggccgcacctgcattacgaggcgcgcatcaacgggcagcccgtcaatcctgtttcggtcgcattgccgacaccggaattgacgcaggcggacaaggcggcgtttgccgcgcagaaacagaaggcggacgcgctgcttgcgcgcttgcgcggcataccggttaccgtgtcgcaatcggattgaagtttgaaccggcgacgaaaacaatgccgtctgaaaacctgcaaacaggttttcagacggcatttatagtggattaacaaaaatcagtacggcgttgcctcgccttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttccgtactatttgtattgtctgcggcttcgtcgtcttgtcctgatttttgttaatccactatgcagttgattaaaacaaaactaagccaaggaagcactgccgtcattcccgtacgggcgggaatcctgacaccacggcacggaaacccatccgctgtcattcccacgaaagcgggaatctagaaatacaacgcggcaggagtttatcggaaatgactgaaacccaacgtaccggattcccgctttcgcgggaatgacgaagtgggcgggaatccggatttatccgttccgacagtgtttgcaaataaaagaaaacccaaccgtcccgattcccggcagggctgttttacggattttgcagcgagggcgcggggcggtcttgcgcctgtttggtttgcagggttgtcagttttttcgtcagcagattcagtatcacgccgtaggcgggcaggaagaagagggtgcagacggtaagtttgaacaggtaatcgacaaaagcgatgccctgccagtttgccgccataaatccatcgctgcttgcgtagaaggcaacggcgaaaaataccagcgtatccaaggcgttgccgatgacggttgatgcggtcggtgcaatccaccacgctttcagacggcgtaatttgttgaatacaaaaatatcaaggatttgtccgatcgcgtaggcggcaaagctggctaaggcgatgcgtccgacaaaggtgttgaattcggacagcgcgcccaagcctgtccaactgccgttgtggaacaaaacggaaaagacgtaggaaagcaaaagggcggggaacatcacccaaaagataatccgccgtgccaagtgagaaccgaaaatgcggacggtcaggtcggtggcaaggaagatgaagggaaaggaaaatgcgccccaagtggtgtggatgccgaaaatttggaaagggaactgcaccagatagttgctggcggcgatgatgaggatatgaaaaagcaccagccggaagagtgccttctgttgctgtgcggcggtaaatgcgtacataaaaatctttcggaaaggcgttcagacggcatatcgtatcgaaggaatgccgtctgaaatatgggaaggatggtttattgtgcgtcgtgctcaaacaagcgtttgcgtgccaatgtttcgaactcggtgcctgcttttccgtagttggcaaacggatgaatggcgatgccgccgcgcggtgtgaactcgccgaatacttcgatgtatttcggatccatcagggcaatgaggtctttcatgatgatgttgacgcagtcttcatgaaaatcgccgtggttgcggaagctgaagaggtagagtttcagggatttgctttccaccattttgatgtgcggaatgtagcggatgtagatggtggcgaagtcgggctgcccggtcatggggcagaggctggtgaactcgggacagacgaatttgacgaaatagtcgttgtcgggatgtttgttgtcgaatgcttcgagaatttcaggcgcgtagccggtcggatattgggttttttgattgcccaaaagagagatgccttgcagctcttcgttgttgcgggacatgagggtttccttagttttttaatgtgggaggttttcgaaccacgggcggcgattgtaatataagcggcggtatctgtgtagttttcttcagacggcatggtttggacggcggcgttttccgtgtcatatatagtggattaacaaaaaccagtacggcgttgcctcgccttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttccgtactatttgtactgtctgcggcttcgccgccttgtcctgatttttgttaatccattatataaacgaaatatattttcagttttgccgcctgaagcgttgttttttgaatattgcatctaaaatactgacttgattgcgttattgcgcggatatagaatctgcttcctattgaaagaacattgtttatatgaaatcaggaaattcggaacccaatcttatggatacgcacacggacgaaacaaaacttcaaaacacgcaagccaaacgcaaacgccgcctgacggcattgacgctgctgttcgcgcttgccgccgcagccgccgggtcggcgttttttttatggtggcagcacgaagaggaaacggaagacgcttatgttgccggacgcgtggttcaggttacgccgcaaaagggcggtacggtgcggaaggttttgcacgacgatacggatgccgtgaaaaaaggcgacgtgctggcggtattggacgacgataatgatgtgctggcttacgagcgggcaaaaaacgagctggttcaggcggtgcggcaaaaccgccggcaaaatgccgccacttcgcaggcgggggcgcaggttgccttgcgccgggcggatttggcacgcgcacaggatgatttgcgccgccggtctgctttggcggaatcgggcgcggtgtccgccgaagagctggcacacgcccgtgcggcagtgtctcaggcgcaggcggcggtcaaagcggctttggcggaagaatcttcggcacgtgcggctttgggcggtcaggtttctttgcgcgaacagccggcggttcagacggcaatcggcaggttgaaagatgcgtggttgaaccttcagcggacgcaaatccgcgcgccggcggacggtcaggtggcgaagcgttcggtgcaggtcgggcagcaggtggcggcaggcgcgccgctgatggcggtggtgccgctgtcggatgtgtgggtggatgctaattttaaagagacgcagttgcggcatatgaaaatcggacagcctgccgagctggtgtccgatttgtacggcaaacaaattgtttatcgcggcagggtggcaggtttttcggcaggtacgggcagcgcgttttcgctgattccggcgcaaaacgcaacgggcaactggattaaagtggtgcagcgcgtccccgtccgtatcgtgctgaaccgcgaagatgtggacaggcatccgttgcgtatcggtttgtcgatgacggttaaagtggatacttccgccgcaggcgcgcctgtttcaaaaacgccgggtgcggcattgccggaaatggaaagtaccgactggtcggaagtcgatcggacggtcgatgaaatcctcgggcaatccgcgccctgatgccgtctgaaacggaggacacaatggattatccaccgcttaagggtgcggcattggcgtgggttacgctgtctttggggcttgccgtatttatggaagttttagatacgactatcgccaatgtcgccgttcccgtcatcgccggcaacctcggtgcggcaaccactcaggggacgtgggtcatcacttccttttctgtggcaaacgccgtttccgtgccgctgacgggctttttggcaaaacgcatcggcgaggtcaaattgtttaccgccgccgctgtcggtttcgtcatcacatcgtggctgtgcggtattgcccccaaccttcagtcgctggttgttttccgcatcttgcagggctttatcgccgggccgctgattcccttgtcgcaaagcctgttaatggcatcctatccgcccgcaaaacggacgctggcactggcattgtgggcaatgaccgtcgttgtcgcccctgttctcgggccgatactcggcggctggatttccggaaactggcattggggttggattttcttcattaatatccctatcggtatcatatcggcatggattacatggaaacatttgaaatatcgggaaacggaaaccgttaaaatgccgaccgactatgtcgggcttacattgatggtagtcggtatcggcgcgttacagatgatgctggacaggggtaaggaactcgactggttcgcctctggagaaatcattaccttgggcgtagtcgcactggtgtgcttgtcgtattttattgtttgggaattgggagaaaaatatccgattgtcgatttatcgctgtttaaagatcggaattttaccgtcggcgtcattgccacgtcattgggttttatggtgtatatggggacgctgaccctgctgccgttagtgttgcagaccaacctgggctatacctccacgtgggcagggcttgccgccgcacctgtcggcatcctgcctgttttcctgtctccgttaatcggcaggttcggcaataaaatcgatatgcgcctgttcgtaactgccagcttcctgacctttgcctttactttctattggcgtacggatttttatgccgatatggatattggcaacgtcatctggccgcagttttggcagggtgtcggtgtcgccatgttttttctgccgctgaccaccatcacactgtcgcatatgaagggcgggcagattgccgccgcaggcagcctgtcgaatttcttgcgcgtgctgatgggcggtgtcggcgtatccgtcgtcagcaccctgtgggaacggcgcgaagcgttgcaccacacacgctttgccgaacacatcacgccctattccgcaacattgcacgaaacggccgctcatttgtcccagcacggcgtttccgacattcaaaccctaggcatcatcaacaataccattacccagcagggttttattatcggctcgaacgaaatctttatggcgggcagcttgttattcattatcatgatacccgtcatatggctggcaaaaccgccg

附录Bttccacaacggcggcggcggtggacattgagggatttgaaaacttgaaatgccgtctgaaaatactggaaatatgttcggacggcattttgaatgcagcagttcccgaaatccgctataatcgcgccccatctgtttcgcacctgcaaacgttccacagatgcgacaatcggaaggattatccgcgcaaaacagccgtttttctttaaaacacttgaactaacactgtttttcgtggtataaatcgcgttttactattttagaagtttggagactgattatggcacgagtttgcaaagtgaccggcaaacgcccgatgtccggcaacaacgtatcgcacgccaacaacaaaaccaaacgccgttttttgcccaacttgcaatcacgtcgtttttgggtagaaagtgaaaaccgctgggttcgcctgcgcgtttccaacgctgcactgcgtaccatcgacaaagtaggcattgatgtcgtattggctgatttgcgtgctcgcggcgaagcttaatttaaacactatttaattaaggattactgcaatgcgcgataaaatcaaactggaatccagtgcaggtactggtcacttctacaccactaccaaaaacaaacgcactatgcccggcaaattggaaatcaaaaaatttgacccagttgcccgcaaacacgtagtgtataaagaaactaaactgaaataatttcagtttgaaagcaaagcctccgactgctcggaggctttgttatttttatcgtgtttcctttccgcttgaaacatctgccgtatgcgaatctgctgcaaaccgtctgccaaggatatgaaaaccgcaaaacggttcataacacaaaaatgccgtctgaaacgtttcagacggcatttcggcagttttcaaccggtcagttgtttggtgatcagtttcttcagcggtgggaaattgttgctggcacgcaataccaagccgcgcaacagttttgccggtgcggtctcattggtaaacagtttcagcatcatattggttccgtgataaagcggatgggcgtgcagcatatgtttgctgctgtatttttccaataatgaagatgcaccgatgtcttgaccgcgctgttcggcttcgagtatcagttttgccaaaatatctgcgctggaaagccccaagttgaaaccgtgtgctgtaacggggtgcataccgacggcggcatcgccaatcagcgcgctgcgtttgccgtagaaacgtttggcaatcatgccgacaagggggtaatggtggatgctgctgaccaattccatatcgccgagcctgcccttgagctgttcttttacgcttgccgccaattcttcgggcgaaaggttttgaacgctgttgattttatcggtatcgacggtaatgacggtattggtcaggtgctcttccagcggcagcagtgcgatggtgcgtccgtaatggaagcattcgtaagcggtatgttggttggaaagggtatgtttcatacggcagacgaacatggttcggctgtaatcgtgcatatcggaggagataccgagttgtcgacgggtttgcgagaagcggctgtctgccgccaaaagcaggcgtgcagtcagtattttgccgttttccaaaatgacttgtgcttcgttgtcagatgttttgacttctttgacaaccgtatcggtcagaatgctgacattgtcgagttgtgatacgacttcataggcggcgcggcggatattgtggttggaaatcagatagcccaaacagtcggcaggttcgccgcgcgcttcagtcggttggggaaagtggagctggtagtcggaacgtccgttcagcactttggcatcgcgcaaagggtagatttcgttttcgggaattttgtcccacatacccaaacgctgcatgatttcgcgggaaaaatgggtcagggcgatttcgcgtccgtcatatggaggattttgcagaacagtcagtgggctgcgttcgatcagggtaactttcaaaccgctgccggcaagttcggctgcaaaacttaaacccgccgggcctgcgccgacgacgaggatgtcgctgtgtaaactcataaaatatcctttgcatagacggatgccgatgatttcagacggtatttgtaagggtttgaatgccgtttgaactatctgtaacagataggcgattatatcaaaacccactgttgaagaaatatgcaggggagggtgtatgcggatttttactttcagcttaatgtgtatcaaatcgggtgtggggtatgtatagtggattaaatttaaaccagtacggcgttgcctcgccttgccgtactatttgtactgtctgcggcttcgtcgccttgtcctgatttttgttaatccactataaaaagccgcatcgtgaaaagatgcggcttcaggtatcggttggattattcttcagaaccggtgtaaggacggatgctgacagttttacggttcagcgcgcctttggttttgaattcgacataaccgtcaactttggcgaacaaagtgtggtctttgcccatacctacgttgtcgcctgcgtggaatttggtaccgcgttggcgtacgatgatggaacctgcgggaatcagctcgttgccgtaggctttaacgcccaagcgtttggcttctgaatcgcgaccgttgcgggtgctgccgcctgcttttttacttgccatttgtaatgctcctaagttttaaggttaggcgattgccacgatttcgatttgggtgaaattttggcggtggccttggcgtttttggtagtgtttgcggcggcgcattttgaagatgcggactttttcgccacgaccgtgtgccactactttagccgttacttttgcaccttcgataaagggtgcgccaacttttacagattcgccgtcagcaatcatcaaaacttcggtcagttcgatttggctgtcgagttcggctggtatctgttctactttcaatttttcgccgacggaaactttatactgtttgccgccggtttttacgaccgcgtacatactcaactccataagggttatggttaatatccgcacaccattgtgcggaactcggcattgtattgttatttgcctgttttgtcaaagtttgcgcggttcggataaccatatgccgtctgaaaagatgtaccctgatggctttgctgatataattgcccgctatttgaatcagctttcaagcggtatctgccgtttgacggaaacgtaaacctgagagtctgccatgctcgagaatctgccctatttccagcgacatctgcctgaagaccttgccaaagtcaatgaagtcatcaaccgtgcggtgcaatccgatgtcgcactgatttcgcaaatcggtacatatatcatcagcgcgggcggcaaacgcctgcgtccgattatgacgattttggcgggtaaggcggtcggttatgatgacgagaaactgtattcgctggcggcgatggtcgagtttatccacacttccaccctcctgcacgacgatgtcgtcgatgaaagcgatttgcgccgtgggcgggcaacggcaaacaatctgttcggcaatgcggcggctgtgttggttggcgactttttatacacgcgcgcctttcaactgatggttgcctcgggcagtatgcgcgttttggaagtgatggcggatgcaaccaacattattgccgagggcgaagtcatgcagctgatgaacatcggcaatacggacattaccgaagaacaatatatccaagtcatccaatataaaacggcaaaattgtttgaagctgccgctcaagtcggcgcaattttgggcaaggcttcccccgaacacgaacgggcgttgaaagactacggtatgtatgtcggtacggcattccaaattattgacgatgtgctggactattctggcgaaaccgaagaaaccggcaaaaacgtcggcgacgatttggcggaaggaaaaccgactttgcctttgatttatctgatgcgtcagggttccgaacaggttgcgaacgatgtgcgtactgctttggaaaatgcagatcgcagctattttgagaaaatccacgattatgtcgtccgttcggatgcgttggcatattcgataggcgaggcgcgcaaagcagtcgattgtgccgttaccgccttggatgccctgcccgacagcgaagtgaaggatgccatgattcagctggcgaaggaatctttggtcagggtgtcttgaggcgatgaatttcagttttgttcccctgtttctggttacgctgattctgttgggggtggtcagcaacaacaattcgattaccatctcggcaaccatattgctgctgatgcagcagacggcattgatacagtttgtcccgttggtcgagaagcacgggttgaatctcggtatcattcttttgaccataggggttttgagtccgttggtttcaggaaaggcgcaggttcctcccgttgccgaatttttgaattttaaaatgatatccgccgtttttatcggtattttcgtggcttggctggcgggacgcggcgtgccttatgatgggacagcagcctgttttaattacagggctgttaatcgggacggttatcggggtggcatttatgggcggtatccctgtcgggccgctgattgcggccggcatcttgtcttttgtcgtcggaaagggttaaaatctccttttcatttcggctcgccatagttcaacggatagaacgtatgcctcctaagcgtaaaatacaggttcgattcctgttggcgaggtttgacgatttcatttgtctgtttcccgtgttgcgggaagtttccgatataaggcctttcagtgttggagggcttttttgccatctgaaaactttttcttcctgcttgaaaaaccgacctttaggacggtagaatcatgaaatgattttcaggcttcgtaaaagatgttccggcttggaaatctgttgttttatgatatagtggattaaatttaaatcaggacaaggcgacgaagccgcagacagtacagatagtacggcaaggcgaggcaacgccgtactggtttaaatttaatccactataaaagctgtacaggtataacaatgaataaatttggggataaggtcgtatgagcgtaggtttgctgaggattctggttcaaaaccaggtggttactgttgagcaggccgagcattactacaatgagtcgcaggcgggtaaggaagtgttgccgatgctgttttcagacggtgtcatttcgcccaagtcgcttgcggcattgattgcgagggtgttcagttattcgattcttgatttgcgtcattatccgcgccacagggtgctgatgggggtgttgacggaggagcagatggtggagttccactgtgtgccggttttccgtcggggcgacaaagtattttttgcggtttccgatccgacacagatgccgcaaattcagaaaaccgtttctgccgcagggattgaggttgagttggtcattgtcgaggatgaccagttggcgggtttgctcgattgggtgggttcgcgttcgacatcgctgcttcaggagcttggggaggggcaggaggaagaggaaagccacaccctgtatatcgacaacgaggaggcagaagacggccctgttccgaggtttatccataagactttgtcggatgccttgcgcagcggggcatcggacatccatttcgagttttacgaacacaatgcccgtatccgtttccgtgtggacgggcagctccgcgaggtggttcagccgcccattgcggtaagggggcagcttgcttcacggattaaggtaatgtcgcgtttggacatttccgaaaaacggataccgcaggacggcaggatgcagctgacctttcaaaagggcggcaagcctgtcgatttccgtgtcagcacattgccgacgctgtttggcgaaaaggtcgtgatgcggattttgaattccgatgccgcgtctttgaacatcgaccagctcggttttgagccgtttcagaaaaaattgttgttggaagcgattcaccgtccctacgggatggtgctggtaaccggtccgacgggttcgggtaagacggtgtcgctctatacctgtttgaatattttgaatacggagtcggtaaatattgcaacggcggaagaccctgccgagattaacctgccgggcatcaatcaggttaacgtcaatgataagcagggcctgacttttgccgctgctttgaagtctttcctgcgtcaggacccggacatcattatggtcggtgagattcgtgatttggaaactgccgatattgcgattaaggcggcacaaacagggcatatggtgttttccaccctgcacaccaataatgcgccggcgacgttgtcgcgtatgctgaatatgggtgtcgcgccgtttaatattgccagttcggtcagcctgattatggcgcagcgtcttttacgcaggctgtgttcgagctgcaaacaggaagtggaacgcccgtctgcctctgctttgaaggaagtcggcttcaccgatgaggaccttgcaaaagattggaaactttaccgcgccgtcggttgcgaccgttgccgggggcagggttataaggggcgtgcgggcgtgtatgaggttatgcccatcagcgaagaaatgcagcgtgtgattatgaacaacggtac

附录Bggaagtggatattttggacgttgcctataaggagggtatggtggatttgcgccgggccggtattttgaaagttatgcagggcattacttcattggaagaggtaacggcaaataccaacgattaggtttgagaatgaaaatgccgtctgaagcgtgtttgtttcagacggcatttgactttcagggtgtttgccgggaaggcggggcggtcagcggtatgccatgtcgggttcggatatttccggcaaactttccgtttggccggaaaccgtatatttcccgtctgcccatccgcccaagtcgatcagtttgcagcgttgcgaacagaaggggcggaatgcgttttcgggtttccatactactgctgtttgacaggtcggacatttgacttgaaggcgtgtttgccgcgattcagtcattgtgttttccttgtgttggttttgaggcgaaaatccctgaataaaacgcgtgcaggcgcattgttttctcacgcaggcttttgaggctgccgtcattgagcagcacatcgtctgcaagcagcaggcgttcggattcggatgcctgatggctgatgacggccgccacctcgccgcgcgtcagcccgctgcgggccatcaccctgccgatacgtttttccacaggggcacttatggtcaggacacgccgtatcaggctgataaattgacgcttttccgtcagcagcggaatttcgacaatgccgtaagctgcatcagtaaaggtttcttgctgttttttgatttctgagaaaatcagcggcaacatcacggattcgagcaaggcttttcgcgatggggaggcaaagacttctttacgcaatatgtcgcgccgcaacaaaccctgtgtgtcaaaaacggtgtcgccgaacagccgcctgatttccggcagggcgatgccgtctgaagccgtcagcgagtgcgccgccgcgtctgcatcgatgcgcggcacgcccaaatcggcaaaacattgcgcggctgccgatttgccgctgccgattccgccggtcagtccgacccataccgtcatcttacagcaccggatgggtcagccaccagttgaccgcccgccatacggaatcgtttgccgtaaaaattatccagcccgaaactgtcagtgcggggccgaaggcaaaatgctgccccttggcgacgcgcataacgattgccgcgaccaaaccgatcagcgaggaaacaaaaatcagtacgggcaatgcggatatgccgagccacgcgcccaatgcggcaatcagtttgaaatctccgttgcccataccggtttttcctgtgagcagtttatacactgcacataagagccataatgaaccatagccggcgaccgcacctaaaacggcagactgcaaaggcacgaagccgccgtccaaattaaatatcagacccagccaaattaagggcagtgtcatcgagtcgggcaggtattgggtgtccgcatcgataaaggtcagggaaatcagaaacgcggtcagtaccaatccgcccagcgtaatccaagaccagccgtattgccaggcgaccagcccgaacaatacgccggtcagcagctcgattaagggataacgtatgctgattttggtttggcaggaagcgcatttgccgcgcaggagcaggtagctgacaatcgggatgttctgccacgcgcgtatcggcacgcggcatttgggacagcaggaatccggtttcatcaggttgaaggtacggctttcctcttcggtcagcggcaggtttaaatattctttggcaaataccgtccagccgcgttccatcatgaccggcacgcggtaaatgacgacatttaagaaacttccgaccagcagcccgaacaccgctgccaaaggcacggcaaacggcgacaatacagacaaatcagacatattttgttctcaatgtattcaaaacaaaaacaaaccggcgcagagcgaatccgcgccggatctgtgcggcaaatcaggcgaccacgttgcccaaattaaacagcggcagatacatggcgaccagaagcgtgccgatgaccaagcctaaaatcacgataatgatcggctccatcatagcggacagcctgccgaccgcattgtccacctcgtcttcgtaaaattcggcggctttgttgagcatatcgtccaaagaacccgattcctcgccgatggaagacatctgcaacatcatattggggaacagttccgtcgcacgcatccccgaagtcatagacaaaccttggatgacgcgcgtacggatttcccgggtggcttcttcatagattaaattgcccgccgcgccggcagtggagtccaatacatcgaccaaaggcacgcctgccgcaatcagcgtcgccgtcgtcctgccccagcgggcaatcgttcctttgcggacaatgtctccgaaaatcggcatacgcagcagtatggcatccatacgccgttggattttaatcgaacgcgccttcaatttaaggaagccgtatatggcaaagcccagtgcgatcagcaccatccagccgtatgagacgaaaaagtcggacatatccatcactgtttgggtcagtgcgggaagctccgcgcccatattggcgtaaacttctttaaaggcgggcagtacgaaaatcatcatcacgaataccaaaccgatggcgacggcgatgacggataccggataggtcagtgcggtttttacctttttgcggatggcctgggttttttctttgtaaattgccaatttgtccagcaggctttccaatacgccgcccgtttcgcccgccgcaaccagattgcagtagaagcggtcgaaatattttgggtggtttgagaatgcgcggctcaacgagctgccctgttccacttcgcctcggatttccatcagcatttccgtcatagacgggttgccgtgtccgcgcgccacgatttcaaatgcctgcatcagcggcaggcccgctttaatcatcgtggacagctggcgggtgaaaacggtgatgtcttcttgtgtgattttgcgcttggagcttgttttcacacgggtaatctgcaacgggcggatgccgcgttttgccagttttttgcgcgcctcttcttcggtaaacgcggatacttcgccgttgaccagtttgtcggaggcggaatgcctgccttcaaagataaagcgtttttctttctttgcgaacaaagaaaatcctccgtttttagccatattctagccccgtaaagtaattggaataaaatgtaagaaacatcgttaaaaaacagtaccggcgtgttcccggtaagatgaaaaccgccgacatcccgcctgcgggcggcaaacgggacagaatcggatgcgattataccttatttaggcggctgtccggcatttatgcgtacacaataaatcttgcaggatattgttgcgggtcaaatgccggccggagggcatttccgccatatggaaataaggtgctattggacgcggcgggcggtgttccggagattcgccaaagccgctgccgtttgttaaactacattctgctacattttaatccggttctgaaaaatcaaggaaaacagatgaatgcttttacccgtgcatggtatgcgctcgaacgccattatcaggatacgcgtcatgtccttttgcgcgaccgctttgcctgcgaaccggaccggtttgagcgtatgcacgagcgtttggacgggatgttgttcgattacagcaaaaaccgtttgggcgaagatacgctgcaactgctctgcaatcttgccgacgcggcggatttggaagggaaaatgcgtgctttgcggacgggtgcgaaagtcaacggcagcgaggggcgtgccgcgctgcatacggctttgcgcctgcccgacggtgcggatgccgtttatgtggacggcagggacgtgttgcccgaaatccgccgcgagttaaatcgtgcgttgaagtttgcacacagtttggacgacggttcgtatcaggggataaccggaaaacggattacggattttgtccacatcggcataggcggatccgacctcgggccggcaatgtgcgtgcaggcacttgagccgttcagacggcatatcaccgtccattttgccgccaacgccgatcctgcctgcctggatgcggttttatgccgtctgaaccccgaaacgacagtgttttgcgttgccagcaagtccttcaaaacaccggaaaccctgctcaatgcacaggcagtcaaggcgtggtatcgcggtgcagggttctcggaatccgaaacggcgtgccatttttgcgcggtgtctgccgacactgcggcagctgcggcttttggtatcgcggcggaacgcgtgtttgcgatgtacgactgggtgggcggacgctattccgtctggtcgcccgtcggtttgcccgtgatggttgcggtcggcggggcgcgtttccgcgagttgttggcgggggcgcacgcgatggacaggcattttttcagtacgccgacgcgtcataatatccccgttttaatggcactgattgccgtgtggtacaacaatttccagcacgcggacgggcagaccgccgttccgtacagccacaacctgcgcctgctgccggcgtggctgaaccagctcgatatggagagtttgggcaaaagccgcgcttcagacggcagtcccgccgtgtgcaaaacgggcggcatcgtgttcggtggtgaaggggtcaactgccagcacgcctatttccaactgctccaccaaggcacgcgcctgattccctgcgattttatcgtcccgatgacggcgcagggcagagaggacggacgcagccgttttaccgttgccaacgcctttgcccaagcggaagccttgatgaagggcaaaaccttggacgaagcacgcgccgaactggcagatttgcccgaagcggaacgcgaacgcctcgcgccgcacaaagagttccccggcaaccgccccagcaacagcattttgattgaccgcctcacgccctacaatttgggtatgctgatggcggcttacgaacacaaaaccttcgtccaaggcgcgatatggaacgtcaaccccttcgatcagtggggggtggaatacggcaaacagttggcaaaaaccatcatcggcgaactggaaggcggcacgtccgtacacgatgcctcgaccgaagggctgatggcgttttaccgcgaatgccgtctgaaaggcggcggcgcggcataaaagtactgccgcctttctgtattgattcgggcgcggaaaaggcaatacctgccgcctgcccgattccgaaacgccaatgtttggcaaccgctcgcgtattgctgacgaatatgcgtttgcgtggcacaatagcgcattcatttcaaatgaacatactgcttgaaaataccggcaagcgtcccacgaaacatctcacataaggaaatattatgtctttgcaaaacattatcgaaaccgcctttgaaaaccgcgcggacatcaccccgaccaccgttactcccgaagtcaaagaagccgtgttggaaaccatccgccaactcgattccggcaaactgcgcgttgccgaacgtttgggcgtgggtgagtggaaagtcaacgaatgggcgaaaaaagccgtgttgctgtccttccgcatccaagacaacgaagtcctcaacgacggcgtgaacaaatacttcgacaaagtgccgaccaagtttgccgactggtctgaagacgagttcaaaaacgcaggcttccgcgcagttccgggtgcggttgcccgacgcggcagctttgtggcgaaaaatgtcgtgctgatgccatcttatgtcaacatcggcgcatacgtcgacgaaggcgcgatggtcgatacttgggcaaccgtcggctcttgcgcgcaaatcggtaaaaacgtgcacttgagcgggggcgtcggcatcggtggtgtactcgaacccctgcaggccgcacccaccatcattgaagacaactgcttcatcggtgcgcgttctgaaatcgttgagggcgtgattgtcgaagaaggcagcgtgatttctatgggcgtgttcatcggtcaatccaccaaaatctttgaccgtacaaccggcgaaatctatcaaggccgcgtaccggcaggttcggttgtcgtatccggcagtatgccttccaaagacggcagccacagcctttactgcgccgtcatcgtcaaacgcgtggacgcgcaaacccgtgcgaaaaccagcgtcaacgaattgttgcgcggcatctgatgccttaaaccgtatttgaaacgtccaatgccgtctgaaatccgcttcagacggcattgccgtttgcacgctgcaacgtgaaaacacagaaacagggacaatttgctataatcaacggtttagaacgaaccgaacactatttgaaggatacaaaatgggttttctgcaaggcaaaaaaattctgattaccggcatgatttccgagcgttccatcgcttacggcatcgccaaagcctgccgcgaacaaggcgcggaactggcgtttacctacgttgtggacaaactggaagagcgcgtccgcaaaatggcggcggaattggattccgaacttgtattccgctgcgatgtcgccagcgacgacgaaatcaaccaagtgttcgccgacttgggcaaacattgggacggcttggacggtttg

附录Bgtgcattccatcggttttgcgccgaaagaagccttgagcggcgacttcctcgacagcatcagccgcgaagcgttcaacaccgcacacgaaatttccgcatacagcctgcccgcgttggcaaaagccgcccgtccgatgatgcgcggcagaaattccgccatcgtcgccctgagctacttgggcgcggtgcgcgcgattccgaattacaacgtgatgggtatggcaaaagccagccttgaggcaggcatccgctttaccgctgcctgtctgggtaaagagggcatccgctgcaacggtatttccgccggcccgattaaaacgcttgccgcctccggcatcgccgatttcggcaaactcttgggacacgtcgccgcccacaacccgctccgccgcaacgttaccattgaagaagtcggcaataccgccgccttcctgctgtccgacctgtcgtccggcattaccggcgaaatcacttacgttgacggcggttacagcattaatgccttgagcaccgagggataatccgccgttttcaaatccgtgcgccgtccgtgccgcatatcggtttcgggcggcgttttgccgtctgaagcgtatttctagggaaatgcccgacttacggcaggcgggatgggaaatgcggacgcttgttttaaccgattgcctttgtgccgacttgctgcaggtgcagcggaaacggttcggatgcgaaaatgccgtctgaaacgccaaacgggtttcagacggcattttttatttaaagcatcagcacacttcaaccagccagccgtatttgtcttccgccaaaccatactggatgtcggtaatcgccttacggatggcatagccgcgttcttggcttttcacttcgatttctttgccgccgatgacgaaggaagtaacgggcgagatgacggctgccgtaccggtcaaaatggcttccgcaccgttttccaccgcagctttgagttcgtcaaccgtgaaattgcgttcgctgacggtatagcccaaatctttggcaaccgtcagtacggaatcgcgggttacgccgtgcaaaaactcgtcggtcagcggtttggtaatgatttcatcgccgttaatcaggataaagttggacgcgccggtttcctgcacgtcgccgttcgggcagaacaggacttgatttgcgccatattcggctttcgccttcagcacccagtgcatggcggaagcgtagttgccgccgcatttgacgcggcccatatgcggggcgcagcggatgtgttcggtttccaccaaaattttgacgggcgatccgactttgaaatagtcgccgacgggggaagccaaaatatacagcagggcggtttcggaaggagaaccggccttgccgataacgggatcggtaccgattaaggtcggacgcaggtacagggcggcaggcgcatcgggaatttcatcggcggcacgtttgaccaatttgattagcgcgtcaagataagcttcggtttcggggcgcggcaggtgcaaaatgtccgcactttgccgcatacgcgcgatattggcagtcggacggaacagcacgattttgccgtctgcctgacggaaggctttcagtccctcgaaacattcgctgccgtagtgcagggcgtgcgcgcccggtgcgagggagaggtcttgggaagattgccattcggtcggctgccatttgccttcgcggtaggcgaggacgggcatttgactgtgaaaaacgctgccgaatacggcgggtacgggtctgctcatgatgtaaagcctttcttattctgatatgtttcaatgaacggtttgaatttgaagattgtaaagatacgcctgcaaacagggttttgacaagtgcgcggcgggtttttctgtcgatgcggtgtccaatccgttatttttcaaatggaaaggaacggtgtatttggtaaaattgtcggcaatcgcatactccgtatgtcgtccgaacacgctgccgcatcctatccgaaaccgtgcaaatcgtttaaactagcgcaatcttggttcagagtgcgaagctgtctgggcggcgtttttatttacggagcaaacatgaaacttatctataccgtcatcaaaatcattatcctgctgctcttcctgctgcttgccgtcattaatacggatgccgttaccttttcctacctgccggggcaaaaattcgatttgccgctgattgtcgtattgttcggcgcatttgtagtcggtattatttttggaatgtttgccttgttcggacggttgttgtcgttacgtggcgagaacggcaggttgcgtgccgaagtaaagaaaaatgcgcgtttgacggggaaggagctgaccgcaccaccggcgcaaaatgcgcccgaatctaccaaacagccttaagaaagccgatatggacaacgaattgtggattatcctgctgccgattatccttttgcccgtcttcttcgcgatgggctggtttgccgcccgcgtggatatgaaaaccgtattgaagcaggcaaaaagcatcccttcgggattttataaaagcttggacgctttggtcgaccgcaacagcgggcgcgcggcaagggagttggcggaagtcgtcgacggccggccgcaatcgtatgatttgaacctcaccctcggcaaactttaccgccagcgtggcgaaaacgacaaagccatcaacatacaccggacaatgctcgattctcccgatacggtcggcgaaaagcgcgcgcgcgtcctgtttgaattggcgcaaaactaccaaagtgcggggttggtcgatcgtgccgaacagatttttttggggctgcaagacggtaaaatggcgcgtgaagccagacagcacctgctcaatatctaccaacaggacagggattgggaaaaagcggttgaaaccgcccggctgctcagccatgacgatcagacctatcagtttgaaatcgcccagttttattgcgaacttgcccaagccgcgctgttcaagtccaatttcgatgtcgcgcgtttcaatgtcggcaaggcactcgaagccaacaaaaaatgcacccgcgccaacatgattttgggcgacatcgaacaccgacaaggcaatttccctgccgccgtcgaagcctatgccgccatcgagcagcaaaaccatgcatacttgagcatggtcggcgagaagctttacgaagcctatgccgcgcagggaaaacctgaagaaggcttgaaccgtctgacaggatatatgcagacgtttcccgaacttgacctgatcaatgtcgtgtacgagaaatccctgctgcttaagtgcgagaaagaagccgcgcaaaccgccgtcgagcttgtccgccgcaagcccgaccttaacggcgtgtaccgcctgctcggtttgaaactcagcgatatgaatccggcttggaaagccgatgccgacatgatgcgttcggttatcggacggcagctacagcgcagcgtgatgtaccgttgccgcaactgccacttcaaatcccaagtctttttctggcactgccccgcctgcaacaaatggcagacgtttaccccgaataaaatcgaagtttaaccaccaccgaaaggaacacaaaaaatgcgcttactccatactatgctccgcgtgggcaatctcgaaaatccctcgatttctaccaaaacgttttgggtatgaaactgctccgccgaaaagattatcccgaaggcagatttacccttgccttcgtcggttacggcgatgaaaccgacagcacggttttggaactgacgcacaactgggatacggaacgatacgacttgggcaacgcctacggacacatcgcggttgaagtggacgatgcctacgaagcctgcgaacgtgtgaagcggcagggcggaaacgtcgtccgcgaagccggcccgatgaaacacggcacaaccgtgatagccttcgtcgaagaccccgacggatacaaaatcgagttcattcaaaagaaaagcggcgacgattcggttgcctatcaaactgcctgataccgccgccgccaatgccgtctgaagcctttaggggtttcagacggcattttgttgccgtcgacctgctgtttgagcctgtgccggttcaaactttatccgttacaccgataaggcaaaaaagatgccgtctgaaacggcatccttgatctgcgaaagggcagttgggaatcaaatacccaattcctgcgccaatgcttgggcacgtttgagtacgtcgccttccgcttcttccagcaatttctgcactgtctcggcagcggcatcgcggtcgccgatttcgagatacatttcggcaaggtcgtatttcgcttcggaaggcgcgtcagaacctacagattccgaagggaaactggtatctgcattatttgggatattttcttccgagaggtagatgctccaatctaccgtttcctcctcgccgtctttcaggaagtcgggcaaagcgtctgcctcagaggtgttggaatcaggcgtttccaaagtgatttccgctgcattttcctcaacggccggtgcttcagcaggttgcaacagtgcggacaaatcatcggcaacggtttccgctgcattttcctcaacggcaggtgcttcagaaggttgaagtaatgcggacaaatcgtctgcggtggcgttgaaatcgggtgtttcggcaacggtttccgttacattttcctcaacggccggtgcttcagcaggttgcaacagtgcggacaaatcatcggcaacggtttccgctgcattttcctcaacggcaggtactttagaaggttgaagtaatgcggacaaatcgtctgcggtggcgttgaagtcgggtgtttcggcaacggtttccgttatattttcctcaacggacggtgcttcggcaggttgaagcaatgcggacaaatcgtctgcggcggcgttgaaatcgggcgtttcaggcgcagtttccgcgacggcatcggtttcgtacactttcaggaaatcgtgcaactcttccggtgtttggacttcggcaactgttttttccaagatggtttcgggcgaggaagccttcaggaagcctgccagtccggagggtgaggcaggttttgcggaagctgtttcttctgtgccgatatggttgtttgagggcaggttgtcggagaaatcggtatcgacggtttccggtttgttttcggcagtttgggcgacagattccggttcgggcgtgtcgatgacgatttcgacagggttgtacgggttgaaggtctcgggctcgtacacgctgtctgtggattcgatggcgttccaatcggcatccgcgcgtttttgggtttcttcatcctgcgtaagtgcgccggataaaatgccgttttgcgcggctgccaggctgtcgaaatccaagtcgatgcggttggaaggcgtatcggtttcgacatcgaacgtttgttttgccgataactcttcttcagattccccatctaaggcaagtgtgtcgtttacatcgtttttcggagcgggttcgggcgttgccggagtttcgacttcggcaaaggtgatttctatgccgtcgtctgccgcgtcgtcaaggtcaggctcttcctcagggacggattcttcggtacggcgcgcgcgtttggattgggcaaggcgcaaaagcagcagcagggcgattaatgccgcgcctccgccggcaagcagcaaggtgtacgaaccgccgaacagaccgtcaaacagtccgctttcggtttcttcttcggcagaaacctgttcgacaggttcggaaacggcgttaccggtttcgtcggtcggcgtgtcgatggcagaagcggcggcttcttggggggcggattcggcagcggtttccgatgcggcagtatttgcagcgggtacaggttcgggtcgaacggccggtttttccgcttttgcttcgggcgcggcaacttttgcttcaggtttttcaaccggtttctctaccgttgcctgtttggacggttcggacggcatggatgcggtttcggctttgggtttcgccgtttgcggtttgggttgttccgctttgatcctgttcagattcggaatgtgaagcacgctgcccgcacgcagtctgccgtgtgcggaaacatttgggtttgccttcagcagcgcatcggcaacctgttcgagcgtcaggtgtttcgggcggatggcggcggcaatctgtttgaccgtttcgcctttgcggacggtatgggttttgccgttgtatgccggtttgacggctgcgttcgcgctgtcttttttatcggttttgcggagggctttggcgttttgattttcttgggactctgctgtcggagcggttttgcggtgtgtcttgccgtctgaaagtgcagatttggttttgggcgagtagccgacaggatcgaggatggcggtgtattcgcgtacctgtgcgcctgcgccgatgcggaacaccaggacgggatcgcggactgcctgttcggaagaaacggcaatgacggctttgtcgcccaacttgtggactttggcggtcaggcctttttcggaaacggtaacgctgccgccgcctagcagggctttggcttcttcgccggttacggtaatgctgccggaaaagggttcgtcaaggttggactggatattcagtccgcccagtccagcatgtgcctgaaaggatgcggcaac

附录Btgcgacggaggcggcaatcagtttgatttgtctgttgtttttcaagatgtatcccctgtgggttggcggctgaatacggtttgaccgcgtacagtctgtaaatttcgtcatcatcgggcatcggcggggcagtcggccggcgggcatttaatatgtgaatgtaccgaccgccgccacattttaaacggcaatcattcgccgtttttacaaattatgacatatctccatcttttttcaaaaacatctgtgcatatttgcatcaatcaaaacaaaatttgttggttttgcaggtgcaaaaacagggttctgcctgtatgattagcgtttatttgatttgctttctcatttggatatgaaattcgtcagcgaccttttgtccgtcatcctgtttttcgccacctataccgttaccaaaaacatgattgccgcaacggcggtcgcattggttgccggtgtggttcaggcggcttttctgtattggaaatataaaaagctggatacgatgcagtgggtcggattggtgctgattgtggtattcggcggcgcaaccattgttttgggcgacagccgcttcattatgtggaagccgagcgttttgttttggctgggcgcgctgttcctgtggggcagccacctcgccggtaaaaacggcttgaaggcgagtatcggcagggagattcagcttccggatgccgtatgggcgaaattgacgtatatgtgggtcggtttcctgatttttatgggtatcgccaactggtttgtgtttacccggttcgagtcgcaatgggtcaactataaaatgttcggctcgactgcactgatgcttgttttctttattattcagggtatttatctgagtacctgtctgaaaaaggaggattgactgtggaatattttatgttgctggcaacagacggggaggatgtgcacgaggcgcgtatggcggcacgtcccgaacacctcaaacggctggagacgctgaagtcggaaggccggctgttgacggcaggcccgaatcctttgccggaggactccaaccgcgtttcgggcagtttgattgtggcgcagttcgagtctttggatgcggcgcaggcttgggcggaagacgatccctatgttcatgcaggcgtgtacagcgaagtgctgatcaagccgtttaaagcggtgttcaaataatgccggccgtcgatttgatccgcgaacgcctgcagacgctcgatccgctggtgttggaaatcggcgatgagagccatctgcacaaaggacacgcgggcaataccggcggcggacattatgccgttttggtcgttagcggccgttttgaaggcgtaagccgcctgaaccgccagaaaacggtcaaatcgctgctcaaagatttgttttcaggcggcatgattcacgcgctcggcatccgggcggctacccctgacgagtatttccatacggcggactgaatgaagtctgcccgaacatttcaatttaaaatttaaagagagaagattatgaaagcaaaaatcctgacttccgttgcactgcttgcctgttccggcagcctgtttgcccaaacgctggcaaccgtcaacggtcagaaaatcgacagttccgtcatcgatgcgcaggttgccgcattccgtgcggaaaacagccgtgccgaagacacgccgcaactgcgccaatccctgctggaaaacgaagtggtcaataccgtggtcgcacaggaagtgaaacgcctgaaactcgaccggtcggcagagtttaaaaatgcgcttgccaaattgcgtgccgaagcgaaaaagtcgggcgacgacaagaaaccgtccttcaaaaccgtttggcaggcggtaaaatatggcttgaacggcgaggcatacgcattgcatatcgccaaaacccaaccggtttccgagcaggaagtaaaagccgcatatgacaatatcagcggtttttacaaaggtacgcaggaagtccagttgggcgaaatcctgaccgacaaggaagaaaatgcaaaaaaagcggttgccgacttgaaggcgaaaaaaggtttcgatgccgtcttgaaacaatattccctcaacgaccgtaccaaacagaccggtgcgccggtcggatatgtgccgctgaaagatttggaacagggtgttccgccgctttatcaggcaattaaggacttgaaaaaaggcgaatttacggcaacgccgctgaaaaacggcgatttctacggcgtttattatgtcaacgacagccgcgaggtaaaagtgccttcttttgatgaaatgaaaggacagattgcgggcaaccttcaggcggaacggattgaccgtgccgtcggtgcactgttgggcaaggcaaacatcaaacctgcaaaataattctgaaaacgggatatggcggcaagacgttcagacaggcgttttgccgccgcgcaggacagggaataccatgaaacagaaaaaaaccgctgccgcagttattgctgcaatgttggcaggttttgcggcagccaaagcacccgaaatcgacccggctttggtggatacgctggtggcgcagatcatgcagcaggcagaccggcatgcggagcagtcccaaaaaccggacgggcaggcaatccgaaacgatgccgtccgccggctacaaactttggaagttttgaaaaacagggcattgaaggaaggtttggataaggataaggatgtccaaaaccgctttaaaatcgccgaagcgtctttttatgccgaggagtacgtccgttttctggaacgttcggaaacggtttccgaagacgagctgcacaagttttacgaacagcaaatccgcatgatcaaattgcagcaggtcagcttcgcaaccgaagaggaggcgcgtcaggcgcagcagctcctgctcaaagggctgtcttttgaagggctgatgaagcgttatccgaacgacgagcaggcttttgacggtttcattatggcgcagcagcttcccgagccgctggcttcgcagtttgccgcgatgaatcggggcgacgttacccgcgatccggtcaaattgggcgaacgctattatctgttcaaactcagcgaggtcgggaaaaaccccgacgcgcagcctttcgagttggtcagaaaccagttggagcagggtttgagacaggaaaaagcccgcttgaaaatcgatgcccttttggaagaaaacggtgtcaaaccgtaatggcatttccaataccgatgccgtctgaagcctttcagacggcattgcacgttcaggtaaggaggacggcttatgcgtgcggtcatacagaaaacggtaggtgcaaaggtggatgtcgtgtccgaagccggcacggaaacctgtggcaaaatcgacggcgggtttgtcgtgttactcggcgtaacgcatagcgacacagaaaaagatgcacgctatatcgccgacaaaatcgcccatttgcgcgtgtttgaagacgaagcgggcaagctgaacctgtctttgaaagatgtcggcggcgcggtgctgctggtgtcgcagtttacgctttatgccgacgcggcaagcgggcggcggccttcgttttcccaagccgcacctgcagaacaggcgcagcagctttacctgcgaacggcggaactgttgcgcggacacgggattcatgtcgaaacagggcgtttccgcacgcatatgcaggtgtcgctctgcaacgatgggccggtaaccatactgctggactctttcatgacgcggatttccccaaaaatgaaggttgttccggattgaaattgaatccgcaatgataaaatatcgacaatgaacgacaatacacacacccttcccccgcgccacctgtccgtcgcccccatgctcgactggacggacaggcactaccgttaccttgcccgccagattacccgaaatacttggctgtacagcgaaatggtcaatgccggtgcgattgtttacggcgacaaagaccgctttttgatgttcaacgaaggcgagcagcccgtcgccctgcaactgggcggcagcgatccgtccgatttggcgaaagccgccaaagccgccgaggcatacggttacaacgaggtcaacctcaactgcggctgccccagtccgcgcgtgcagaaaggctcgttcggcgcgtgtctgatgaacgaagtcgggctggttgccgactgcctcaacgccatgcaggatgcggtcaagattcccgttaccgtcaaacaccgcatcggtgtggacaggcagaccgaataccaaaccgttgccgatttcgtcggcacgctgcgcgacaaaaccgcctgcaaaacctttatcgtccacgcccgcaacgcttggctggacggtctttcccccaaagaaaaccgcgacgttcccccgttgaaatacgattacgtttaccgcctcaagcaggagtttcccgggctggaaatcatcatcaacggcggcatcaccaccaacgaagcaatcgcaggacacctgcaacacgttgacggcgtgatggtcgggcgcgaggcgtaccacaacccgatggtgatgcgcgaatgggacaggctgttttacggcgatacccgcagcccgattgaatacgccgatttggtgcagcgtctctacacatacagccaagcccaaatccaagccggacgcggcacaatcttgcgtcacatcgtccgccacagccttgggctgatgcacggtctgaaaggcgcgcggacttggcggcgtatgctttccgacgcaacgctcttgaaagacaacgacggcagcctgattctcgaagcgtggaaagaggtcgaacgggcaaatatgcgcgaatagggcggggctgtatgtgtgaaatgccgtctgaaggcttcagacggcatttgtgcgtttgtcgggcggtgtttagggggcggtaacggcgtgtttcggcactttgtccatatcccagtgtgccaccgcccagtcgagcagttcggcagggcggtcggtttccggtgcttcgggcagcttgaggtaacggaacacttggcggaggagttgttcgcggcggtttaaatccaatgcgggggcgagcgtctgtttcgaccatttctgcccttgtgcgttggtcagcagcggcaggtgggcatattgcggtgtcggaacgtccaaacactgctgcaaatagatttggcgcggcgtggaaacgagcaggtcttgtccgcggacgatgtgggtaacgccctgttcggcatcgtcggcaacgacggcgagctggtatgcccagtaaccgtctgcacgaagcaggacgaaatcgccgatgtcgcgggcgaggttttgggcgtaaccgccgacgatgccgtctgaaaaaccgataatgcggtcggggacgcggatgcgccacgccggctgtttgccttgcagtgcagggcgttggccggggtggcggcaacgtccgttatagacgaacccgtctgcgccccgccttgccccggcctgccagtctttgcggctgcaatggcagggatagaccagtccggcggttttcaggcggcatagggtttcttcatacagggcgtaacggcggctctgataggcgacttctccgtcccactcgaatccgaatgcctcaagcgtgtgcaggatatggcttgccgcccccggcatttcgcgcggcggatcgaggtcttccatgcggatcagccatttgccgccgtgcgcgcgcgcatcggcataggaagcgacggcggtcagcagcgagccgatgtggagcagcccggtcgggctgggggcaaaacgtcctgtgtacatatctggtacagcccctttatttaagactattaatcaaagccattatctcatctttattcagttccatcccgggctcttcaagcaaggttaaatcatatagggcattatattgctcttcggtagctgaaccatccataagagcaggcgagaaaaaatcaaaggctctatctgcaattctctcattacttgcatttctactaaccagtttcgtcaattctgtatattttgaaaagtttatggaaaaataaaacagcgaaaaagttttggtttcgctgtttttgatttaattagcactgataatcttcaaattcccacgaaaaaaaacgaagtaaataagtcaatgacttttcccaagtttcttttgaacattctttaagaattttctcaatttccgatttaataacagaatgattaaattcattcataatcatcatacccgccccccatttaaccctttgattttggaaacaattatgcaaaatccatttaggagagcatatgcgaacagaaaatatatctgcagcatcactatcatcagttcctatgtctaaatcaattcccacacaaaaattgtctttgatttcgggaacgaaatcttcaaaggcacaatcgtaaagattgatggctttcaattctaggttaatcattttatattcaatagtatggggaggtaccggatccttaaaaatcagatctgaataaatttcattgggtgaaatgatttcgattgcttttgccatgattctatttccttttgtgttagtgggtaatgtcgtgcattaacttcttgcccattaatatttttagggtgaatccttgatatgccgcactgtgtccggtcaaacgggcgatgccgtctgaaagcctttcagacggcatcgggaaaatgcctaagccaaa

附录Bggcgcgagcagtttttcaaacgcttcttcaaactgtttcaaaccgtcttcctgcaaacgcgttgccaaggtttcgacatcgatgccgagcgcggcggtttcggcgagctgcgcttgtgcttcttctacgccttcggtcagcgtggctttggctgtgccgtggtcgataaaggctttgagcgtggcatcgggaacggtgttgacggtgtgcgcgccgatcaggctgtcaacgtagagcgtgtcgggataggccgggtttttcacgccggtagatgcccataaaagctgcacgcggtttgcgcctttggtttccagcgcggcaaattcggggctgccgaagtattgcgcccagtcttggtaggcggctttggcaagggcgatggcgattttgcctttgaggtggtcgggcagtgttgtgtccagcgcgccgtccacacgggagatgaagaagctggcgacaacttggatatgggcaacgctttgtccggctgctaagcgtttggcgatgccgcgcgcgtaggcggcgtaggctttgagggtttgggcgcgtgagaacagcagggtcaggttcacgctgatgccgtctgaaacgagggtttcgagcgcatcgatgcctgcgtcggtggcaggcactttaatcatcgcgtttttgcacccgatggcggcgtagaggcggcgcgcttcttcaaccgtgccttgcgcgtctttggacaattcgggcgaaacttcgaggctgacgaagccggttttgccgccggtggattcgtgttcggcaaggcaaacgtcgcaggcggcacgcacatcggcaaccgccattgtttcgtagcgttgtttggggctgaggttttgctgcttgagggcggcgatttcatcggcgtaaagcgcgtcgccggcgaaggctttttggaagatggcgggattggaagttacgccgcacacgccctgtttcaacatttgcgccaattcgccgctttgcactagcgagcgggaaaggttgtccagccagatttgttgtcctaatgctttaacgtccgataaaatggtcatctctgattcctttggatggataggcggggtttgagggcttatgctaccccgattcggaaattttgggtagttttattacagcaaaggcggatggcaatggcagaaaacggaaaatatctcgactgggcacgcgaagtgttgcacgccgaagcggaaggcttgcgcgaaattgcagcggaattggacaaaaacttcgtccttgcggcagacgcgttgttgcactgcaagggcagggtcgttatcacgggcatgggcaagtcgggacatatcgggcgcaaaatggcggcaactatggcctcgaccggcacgcctgcgtttttcgtccaccctgcggaagcggcacacggcgatttgggtatgattgtggacaacgacgtggtcgtcgcgatttccaattccggcgaaagcgacgaaatcgccgccatcatccccgcactcaaacgcaaagacatcacgcttgtctgcatcaccgcccgccccgattcaaccatggcgcgccatgccgacatccacatcacggcgtcggtttccaaagaagcctgcccgctggggcttgccccgaccaccagcaccaccgccgtcatggctttgggcgatgcgttggcggtcgtcctgctgcgcgcacgcgcgttcacgcccgacgatttcgccttgagccatcctgccggcagcctcggcaaacgcctacttttgcgcgttgccgacattatgcacaaaggcggcggcctgcctgccgtccgactcggcacgcccttgaaagaagccatcgtcagcatgagtgaaaaagggctgggcatgttggcggtaacggacgggcaaggccgtctgaaaggcgtattcaccgacggcgatttgcgccgcctgtttcaagaatgcgacaattttaccggtctttcgatagacgaagtcatgcatacgcatcctaaaaccatctccgccgaacgtctcgccaccgaagccctgaaagtcatgcaggcaaaccatgtgaacgggcttctggttaccgatgcagatggcgtgctgatcggcgcgctgaatatgcacgacctgctggcggcacggattgtatagtggattaacaaaaaccagtacggcgttgcctcgccttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttccgtactatctgtactgtctgcggcttcgtcgccttgtcctgatttttgttaatccactatataaggcgttgcagccgtttcagacggcatttgtggtaagatatgccgtctgaaaacaaggaaatcccatgcaggcaatttctcccgaattacaggcgcgcgccgccaaaatcaaactgttgatcctggatgtggacggcgttttgaccgacgggcgcatctttatccgcgataacggcgaagaaatcaaatcgtttcacacactggacggacacggtctgaaaatgcttcaggcaagcggcgtgcagactgcgattatcacgggccgggacgcgccctccgtcggcatccgcgtcaaacagttgggcataaattactatttcaaaggtatcagcgacaaacgtgccgcctatgaagaattgcgcgcgcaggcgggcgtggaagaagccgagtgcgcctttgtcggcgacgacgtggtcgatttgccggtaatggtgcgctgcggattgccggttgccgtccccggcgcgcattggtttacgcggcaacacgccgcctatatcacggaacacgcgggcggcgcaggcgcggtgcgcgaagtgtgcgacctgattatgcaggcgcaagggactttgggcgcggctttgaacgagtacatcaaatgaaagtaagatggcggtacggaattgcgttcccattgatattggcggttgccttgggcagcctgtcggcatggttgggtcgtatcagcgaagtcgagattgaagaagtcaggctcaatcccgacgaaccgcaatacacaatggacggcttggacggcaggcggtttgacgaacagggatacttgaaagaacatttgagcgcgaagggcgcgaaacagtttccggaaagcagcgacatccattttgattcgccgcatctcgtgttcttccaagaaggcaggttgttgtacgaagtcggcagcgacgaagccgtttaccataccgaaaacaaacaggttctttttaaaaacaacgttgtgctgaccaaaaccgccgacggcaaacggcaggcgggtaaagttgaagccgaaaagctgcacgtcgataccgaatctcaatatgcccaaaccgatacgcctgtcagtttccaatatggtgcatcgcacggtcaggcgggcggcatgacttacgaccacaaaacaggcatgttgaacttctcatctaaagtgaaagccacgatttatgatacaaaagatatgtaagctatttgttttaatagcatttttttcggcgtcccccgcttttgcccttcaaagcgacagcaggcagcctattcagattgaggccgaccaaggttcgctcgatcaagccaaccaaagcaccacattcagcggaaacgtcgtcatcagacagggtacgctcaatatttccgccgcccgcgtcaatgttacacgcggcggcaaaggcggcgaatccgtgagggcggaaggttcgccagtccgcttcagccagacattggacggcggcaaaggcacggtgcgcggacaggcaaacaacgttgcttattcatctgcaggcagcaccgtagtcttaaccggtaatgccaaagtacagcgcggcggcgatgtcgccgaaggtgcggtgattacatacaacaccaaaaccgaagtctataccatcagcggcagcacaaaatccggcgcaaaatccgcttccaaatccggcagggtcagcgtcgttatccagccttcgagtacgcaaaaatccgaataatcccaaaatgccgtctgaaatataaacccggttcggacggcatatgccgaccgaagatattgaagagatatttatgagtgcaaacgtcagccgccttgttgttcaaaacctgcaaaaaagtttcaaaaaacgccaagtcgttaaaagcttctccctcgaaatcgaaagcggcgaagtcatcggactgctcgggcccaacggtgcgggtaaaaccaccagcttctacatgattgtcggactcatcgccgccgacgcaggcagcgtaaccctagacggacaagaattgcgccacctgcccatacacgaacgcgcccgcctcggtgtcggctacctgccgcaggaagcctcgatattccgcaaaatgaccgtcgaacaaaacatccgcgccatcttggaaatcagaaccaaagataaaaatcaaatcgacagggaaatcgaaaaactgctcgccgacctcaatatcggacacttacgccgcagccccgcgccgtcgctgtccggcggcgaacggcggcgcgtcgaaatcgcccgcgtactcgccatgaaaccgcattttattttgttggacgaaccttttgccggcgtcgatccgattgccgtcatcgacatccagaaaatcatcggtttcctcaaatcgcgcggtatcggcgtactgattaccgaccacaacgtacgcgaaaccctcagcatctgcgatcgggcctacattatttcagacggcacggtgttggcatcgggaaaacctgatgatttggtcggaaacgaacaggttcgttctgtttatctgggtaagaacttcaaatattgaaaatatttttcagacgggcgacctaatatcgtcgggcaggcggcaaaaatacggatttatgttgtttttacataaattaattcaaatttaaaacattgacttaaacctgttttcaaagaatattgcccgatatgcttgcatgtcgtcccgtaatttggtttaatacgcatctcttaacgagacagacaaaggccagatagctcagttggtagagcaacggattgaaaatccgtgtgtcggcggttcgattccgcctctggccaccaaaaaaccgccttgaagcggttattttttttgcctgccgtttttgggaagttgtccgtgtcggacacgttttgtgtctgaccgttatgtagaagggcaaaaatgataatgaccgccccgttgcgttttggagaagagggtaaaggcagaaagcatatgccgtctgaatgatatttcagacggcattttatattgcggcggcactcagtccgtgtcgctttcaggcaactctgccgaacccatgcgtttgagcacgatattggttttgttgcggagccgtttgcttttcggatggtcggcgtagtagagcggggcggggacgcgcgccgtcagttttgccgcctgctgtttggtcagcttggcggcgggtatttgataaaaataccgggacgcggcttccgcgccgaaaacgccgtagtgccattcgattgagtttaaatacagttcaaaaatcctgtctttgtcggtaacggcttccatcatcgcggtaatcgccgcttcttcgcctttgcggatatagctgcggctttcgtttaaaaacaggtttttggcaagctgctggctgatggtcgagccgcccgccttcactttgccgctgttccggttgcgcctgatggcgttttgaatgccgccccaatcgaagccgccgtgcccggcgaaacgggcatcttcggaagcaatcagggcttttttcaggttggtggaaatgcgtttgtagggcatccagcggtaatccagtgcgacatcgcgaccttcctgttcaaactgcttcatccgcatcgacataaaggcagtccgatggggcgcgacggcgcggtaggtaatgatgttgccgtacacataggcattgaaaaagataaagatgccgacgggcagggcaatcagccatttgatgatgcggaacatgtttatagggctttcatgtattcgataacggggcggatatcgggcgtaaatccgcgccagagggcgtaggaagccgccgcttgaccgactagcatacccagtccgtcggcagtttttttcgcacccgattgtcgtgcaaaatctaaaaacggttttgccgcgcagccgtacaccatatcgtaggcaagcgcgcagttttgaaaaatatcgggcggaatatcgggaatctgaccgtttagaccgcccgacgtgccgttgatgatgatatcaaaaccgccgttcacgtccgccatcgggacggcttcaatgccgaaaagctgcgccaattcctcggctttggcgcgggtacggttggcaatgacgatacgggcaggacggtgttctttcaaaacaggaatcacgccgcgcaccgcgccgcctgcgcccaaaagcaaaatggttttgccctcgatggcaatatttttgacctgcgtgatgtcgttggtcaaaccgataccgtcggtgttgtcgccacgcagcttgccgtttttcaacggaatcagcgtattgaccgcacctgccgccaatgcgcgttcggaatgctcgtccgccagatgaaacgcttcctgtttgaacggtacggtaacgtttgccccgcaaccgcctgtttcaaaaaatgtcgaaaccgcctgcgcgaaaccgccgatgtcggcgcaaatgcgttcgtattcaatgtcaacgcc

附录Bttcctgaagggcaaattgttgatgaatttgcggcgatttgctgtgggcgacggggttgccgaaaacggcgtagcgggggagggcggtcatggtcgtgttccaaaagacgggaaggctattttataacggcggcgtacagatggaaacgatgccgtctgaaaccgccttcagacggcatcgtttcctgtatcggtcgggaaaaatccggatgcggtgcgccggcttgtccgcattgttgacaatcttgccgtctgaaactatattttccggcttgaaatttgacgcaaaaccggtttcagacggcatcggcgtggtaaaatcgtgccgactttgcgtcaagccgccgcgttccgcatattttgctatttcccttttccaggagctgaaaaatgtctattaaaaacgccgtaaaattgattgaagaaagcgaagcccgctttgtcgatttgcgctttaccgataccaaaggcaagcagcaccactttaccgtgcctgcgcgcatcgtgttggaagaccccgaagagtggttcgaaaacggtcaggcgtttgacggttcgtctatcggcggctggaaaggcattcaggcttccgatatgcagttgcgccccgatgcgtctacagccttcgtcgatcctttttatgatgatgcgactgttgtgttgacttgcgacgttatcgatcccgccgacggtcagggttacgaccgcgacccgcgctccatcgcccgccgagccgaagcctatttgaaatcttccggcatcggcgagaccgcctatttcggtcccgaacccgagtttttcgtattcgacggcatagaatttgaaaccgatatgcacaaaacccgttacgaaatcacgtccgaaagcggcgcgtgggcaagcggtctgcatatggacggtcaaaacaccggccaccgcccgaccgtcaaaggcggttacgcacctgttgcaccgattgactgcggtcaggatttgcgttcggcgatggtaaacattttggaagaactcggtattgaagtggaagtgcaccacagcgaagtcggcaccggcagccaaatggaaatcggcacgcgctttgctactttggtcaaacgcgccgaccaaacccaagacatgaaatatgtgattcaaaacgttgcccacaacttcggcaaaaccgccactttcatgcccaaacccattatgggcgacaacggcagcggtatgcacgttcaccaatccatttggaaagacggtcaaaacctgttcgcaggcgacggctatgccggcttgagcgacaccgcgctctactacatcggcggcatcatcaaacacgccaaagccttgaacgcgattaccaatccgtccaccaactcctacaaacgcctcgtgccgcactttgaagcgccgaccaaactggcatactccgccaaaaaccgttccgcttccatccgcattccgtccgtgaacagcagcaaggcgcgccgcatcgaagcgcgtttccccgatccgaccgccaacccgtatttggcatttgccgccctgttgatggcgggtttggacggcattcaaaacaaaatccatccgggcgaccctgccgataaaaacctgtacgatctgccgccggaagaagatgcattggtgccgaccgtttgcgcttctttggaagaagcactggccgccctcaaagccgaccacgaattcctcctgcgcggcggcgtgttcagcaaagactggatcgacagctacatcgctttcaaagaagaagacgtacgccgcatccgcatggcgccgcacccgctggaatttgaaatgtattacagcctgtaagcacgtctggttttcagaaaagcaatgccgtctgaacacagtttcagacggcattttgcatttgaacggcaaaccggcggcgcggggcggcatttttcagcaggcgggcgatatttgctacaataggcttttgttttttttgggctgcacgaacgatgactgcatcgaaatcaggttttatcgggcaaatcttttcccgcaatatgcttgtctgtatttttacggggtttacctcggggctgccgctgtactttctgattaacctgattccggcgtggttgcgcagcgagcaggtggatttgaagagcatcgggctgatggcgttaatcggtctgccgtttacttggaaatttttgtggtcgccgctgatggacgcggtcaggctgcccgttttgggacggcggcgcgggtggatgctgctgacgcaggcagggttgctggcggctttggcggtctatgcctttttaaacccccgtaatcatctgccgctgattgccggcttgtcggtgcttgtcgcttttttttccgccagtcaggatattgtattggatgcgttcaggcgcgagattttgtcagacgaagaattgggtttgggcaactcggttcatgtgaacgcctaccggattgccgccctgattcccggttcattgagtttggtgttggcagacaggatgccgtggttagaagtatttgttatcacttcattatttatgctgcccggccttctgatgacgctgtttcttgcgcgcgaacccgtgttgcctcctgccgttcctaaaacgttgaagcagaccgtggtagagccgtttaaagaattttttacgcgcaagggcatcgcttcggcggtgtgcgtgctgctgtttatcttcctttacaaactcggcgacagtatggcaaccgcgttggcaacgccgttttatctggatatgggtttcagcaagaccgacatcggtttgattgcgaaaaatgcaggactgtggccggcagtggcggcaggtatcttgggcggtgtgtggatgctgaaaatcggcgtaaacaaagccttgtggctattcggcgcggtgcaggctgtaaccgttttggggtttgtatggctggcagggttcggacctttcgacacggtcggcacaggcgagaggctgatgctggcggcagttatcggcgcggaagcggtcggcgtggggttggggacggcggcgttcgtatcgtatatggcgcgtgaaaccaatcccgcatttaccgcaacgcagcttgcgctgtttaccagcctgtccgccgtcccgcgcacggtcatcaattcctttgccggttatctgattgaatggctcggttatgtaccgtttttccaactgtgtttcgcactcgccctaccgggtatgctgctgctgctgaaagttgcgccttggaacggggagaaaactcaggatgcaggcagatgaacgcgtcaaactggagcgtttacctgatattgtgtgaaaacagcgcgttctattgcggcatcagcccgaatccgcaacagcggcttgccgcccacacaaccggtaaaggcgcgaaatatacccgcgtattcaaaccggtggcgatgcgtatcgttgcaggcgggatggataaaggaacggcactcaggcaggaaatcgccgtcaaaaaactgaccgccgcacaaaaacggcaattgtgggagcaggcagaaaaaatgccgtctgaaacctgacggttcaggttcggacggcagttggcagcaatcagggaaaagcggggcaggcggtaaggaaaaccgacgtttcaacacacaggacggtacataaagcgtcgccctatgaaagtgaaggcatatatcagtattttttatacgccaacagaaaagaatacgatgaactgtttgttggatttgtattgattaatcagtatattttttatgccggggtatttttccttatcggtatcccttcttttatgaggatgcctgccgctcatataaagaacgggaaaatacgatgggaaaatacggtacagccctcgacatcgcacaatatgtcaacttatagtggattaacaaaaatcaggacaaggcgacgaagccgcagacagtacagatagtacggcaaggcgagacaacgccgtactggtttttgttaatccactatatttgtttgttttatattgtaagtatacgtataggctttgtaaaggtaaattgtgaaaaaagcagttttttaaacgaatgaaacggcttcgggctgaaatatatgctgatgccctgtccttcccgtatatcttgtgtgttgtcaaagtgcaggctgctttgaaatcggtattgccatctatgaaccaccactttgttttatttcagcgggcttgagatgtgtataagaatattgttttgaataaatttaaaaaaatgataatcgttattgaagatttttaaaggaaagcgtagagtgccaattctatgaagcaatacggtaagtaacaatgaaaatatctactgcttgggtatagagcatatttcacaacccgtaactattcttgcggaaacagagaaaaaagtttctcttctatcttggataaatatatttaccctcagtttagttaagtattggaatttatacctaagtagcaaaagttagtaaattatttttaactaaagagttagtatctaccatgaatatattctttaactaatttctaagcttgaaattatgagaccatatgctactaccatttatcaactttttattttgtttattgggagtgtttttactatgacctcatgtgaacctgttaatgaacaaaccagtttcaacaatcccgagccaatgacaggatttgaacatacggttacatttgattttcagggcaccaaaatggttatcccctatggctatcttgcacggtatacgcaaaacaatgccacaaaatggctttccgacacgccagggcaggatgcttactccattaatttgatagagattagcgtctattacaaaaaaaccgaccaaggctgggtgctcgaaccatacaaccagcagaacaaagcacactttattcaatttctacgcgatggtttggatagcgtggacgatattgttatccgaaaagatgcgtgtagtttaagcacgactatgggagaaagattgcttacttacggggttaaaaaaatgccatctgcctatcctgaatatgaagcttatgaagataaaagacatattcctgaaaatccatattttcatgaattttactatattaaaaaaggagaaaatccggcgattattactcatcggaataatcgaataaaccaaactgaagaagatagttatagcactagcgtaggttcctgtattaacggtttcacggtacggtattacccgtttattcgggaaaagcagcagctcacacagcaggagttggtaggttatcaccaacaagtagagcaattggtacagagttttgtaaacaattcaagtaaaaaataatttaaaggatcttattatgaatgagggtgaagttgttttaacaccagaacaaatccaaaccttgcgtggttatgcttcccgtggcgatacctatggcggttggcgttatttggctaatttgggtgaccgttatgcggatgatgctgctgcaattgtcggtaaggatgcaaacttaaatggtttgaatttatggatgaaaaaaggggtggaaaacctatgggatgatacggtcggtaaaaagacccgtttaatgtgtatttccgttttttggattgtggttttcaatttgtagcgaatcggattcggcatatacggcattgcaaaaagcgtttgactctccaatgccgtctgaaaaccggtttcagacggcatttgcgttcagtgagaaaggtcgcgcctgccgcccgaacgtctcgccgcagcctctgcataacggcgcacctctttttccaaattttccaagttcaaaggaaaatcaggcagtctgtctccctgtttctcttcgcggacaatccgcccgccatccaaataccacgtctgttgcgcatgataggtctgcatatccgccgttacgccatccgctttcaatgctaccgtcgaagattgtgcaataaaaagatttccgtttttcaaataatattcgaaactctggcgtttttttccattgtcgaaactccaatagactttttgcggcagaccgtccgcatcatagccgaccacaagactgttcgccttcatccctcggggcatcaattcccgcatattctgataaaacacagaattgcgcgagtccgacgcaattcggttgctctctttgcggaagtcccaaaccttctgctcgtcattcgcgacatcccggtatttcgccaaatatacctgggccatctgataacacccgaggcaatgctcataaacatcttccccgattttcccgcgccccgccgcatcaaataccgaaccgtctggttgccaaacaacccgatattctcctgtcgtttcataattttccccgtgaaccgttccgccgtacacatttacagaaaacggacgatcgttccgatacagatattcggcattaacaaatgcttccggcgagcgttgcgaaagcgaaaccgcaaccaaaccgccctcgccgatatggtaatccagccaaacctctttcccatgttcctgctccgttacgtgaaaccatttcgccttttctttcaaacgactgagccggatagcgagcgcgagataatccttctccgactgcaacggaccgtcatccacagttccggcaagattttcctccgtccttatcgattccttcacgatgacaaccgccctgtcggcatttcggaacaggcgggcaagtttcgccacaaaagcattcggat

附录Bttttaggtacttcagttgccgtatcgctcaaaaaccaacgcggattaatctcataggcaatacccgttcccagccaaaaggcaaatacaagtgcaaaaaatgacaacagtaccggtttgaattttttaaacatatttatttttcgtttaacagaatatatcgattatatcagacgagctttgattgccgggttttgctattttttgttgtaataatcaaattgcacgttgactatgtctttctcggtaaaaatataacggagcattgttttaagcctttcataacgttcattaattcctacgctatcaggtagccaaggggaagctttaatttcaaaaagtttccaatttggaaccattaagaaatcaataatggtaccgattccaatgacaacatatcttggtatgtccatcggataaggatatttttttctaacctcgattaaatcattctccaacttccaatattcttcatcatcccacaccccgtcatcataccatttgccaataaatgaattttcgtcatacccctcaaaacaagtaatatttcttctgaagttttttaactcacacataatacacataataattaatctccaatacgatttaggtttttatcaaatgtaccgtttcttgtttcttttctgtaatgttattcatcgtagtaaggttctgttgaataattgtctttgcccccggcaatgatagtaacaattttcccttttgcttcccaagcttgtactcctatttcatcaaactcatagacatatgtcggataagattcatttgataaataatatttatcaacaccgtatgatttagggtaatggaaaagctgtttaaaatcttcaaaattcagacctattatattaacgcccataaaatatagctcctgataacaaaatatcgaaataattttgtttttttttttgacggaaatgagtaaatttgagtcgggagattcataatattctgttctaaactcatcaggaggttcatacataaaagtttccagtatgtttttgtactgtgttatatccgcaccaaaacggaatattcctacagaagtaaaaggtaaaaattcgggagttttaacgaccgcgtcgaccatgctcttctccttttgtttttcgattggcatttttggcaatatttctgattttttgcttaatctttaagcgttcatttttggacattccgggaataattttatttgttaattcagcaatttttgattccgctgatatttgacttcgaccgccatctccatgtttttcattcttggagcttcctgttcttttaggcggacaagaattatgaacccaaaccccttccgtttccgcctgattacccttgacgaagtaagtatgccaatcggcaacggtcagattgtaggctttgagcggttttggtttgacaacggttttgcggacggtttgggttctgccgctttcggataacagcctgcttcccgctttcaaatcttccgctttaatccatttgccgtccgaataaaacggatggatgcggttggaaatcaggatttggctgttgccgatgccgtctgaaagccggatatcgcttcagacggcattttgattgccgggttttgctattttttgttgtaataatcaaatcgcacgttgactatgtctttctcggtaaaaatataacggagcatcgttgtgaatctttcataacgttcatgaattcccacactatcaggcaaccaaggggaagctttaatttcaaaaagtttccaatttggaaccattaagaaatcaataatggtaccgattccaatgacaacatatcttggtatgtccatcggataaggatatttttttctaacctcgattaaatcattctccaacttccaatattcttcatcatcccacaccccgtcatcataccatttgccaataaatgaattttcgtcatacccctcaaaataaggaacgtttcttataatatccttgaactcacacataataatgtatctccaatataattaaacttttcgtctcaatctacctttactatgttgtattggaaagtaaaaaaatttccagtcctctacatctagatcagtaaaaatataacggagcattaccctgaacctttcataacgctcattaattttgacacttttaggcaaccaagtagaagctttaatttcaaaaagtttccaattttgaaccattaaaaaatcaataatggtaccgattccaatcacgatgtcccttggtatatccatcggataaggatatttttttctaacctcaattaaatcattctccaatttccaatattcttcatcatcccacaccccgtcatcataccatttgccaataaatgaattttcgtcatactccttaaaacaagggatgtttcttctaaaatccttgaactcgcacataataattaatctccaatacgatttaggtttttatcaaatgtaccgtttcttgtttcttttctgttcagtttttcgggtgaagatgcctctttccaagcacctccattatgtgaatctacatcgcgtgatatataactctttccttttttaaaaatagcagcatcatttctcgttctttcttttatttttctatatcccaattcctttgctgctgcatatgcttctgaatcattcccatatatgggggtagatggtgtttttcttggcggacaatcattatgaacccaaaccccttccgtttccgcccgattgcccttgacgaagtaagtatgccagtcggcaacggtcagattgtaggctttgagcggctgctgtttgagggtaatgttttgaaccgtctgttttgcaccgctttcggaaagcagggtgtcgccttttttcagacgacctgcctgtatccattttccttgactgtaaaacgggtggattttattggaaatcagggtttggttgttgccgatgccgtctgaaatttcaatgtaaacggtttcttgatacggattgccgtatcgggcggtaacgggtttgtatcccgtttttccgcttgcctcgtccttggcgaagacgcggtcgccggttcggatacgggcaatggctttgtagccgtctgccgttttgaccaaggtgctgccgtggaaggagcaggtgtaggatttttaaagaacttagttctcaatatcctgtttcattcatcaaaatgccgtctgaaagctgaataccgcttcagacggcattttggtggttgggtttttaagccaacctacgcttactgaaaaccaaattgagtttcagacagtttttaggtttgggtgtccaatctaacttatatttgtccatttaattagtcgtttgtatcaaatttccattatttattttccaatttactttataattatcttcataataatctaattcaaaaaaacctgatatttcaatatccaattccattattgttttaatacatttttcaaaataaataatgaaataagattttacgcatgcaccaaaaaaaatatagctgctccaattaaaactatttgtcgggaaaacccacccgcttttatatatttttgcagattctttctcttcgatattaaagggacaattattccaaaaattattaatgttttcttggatgatttttatatcatcgtcagagcattcaatccatccttttatggaaacatatgatgccatgtttaatctcctaaacctgttttaacaatgccgccttttgattcaatatatgacttaacttgtgaatgaacaccgtatttaaaccaaaattctgcacgttttccctgttggtttgctgcttcgatggttgctttaatttgctttctatttttttgatttaagaaatttttaggtttatctattgctgaaattgttcttttggcttgtattaaagcatcattcgtaacagcgtcaatttctctgccgttaataaattttgatgaaccatcagtttttcttctaattaaatcttcataatgtatatctagagcttctctatactttgcattttgatataactgtctcgcactatcagacaaagccaatttctttttataagaatcagcaaaatccccgctaaccgcagccttccctggttttgccgcctttgccaacttcgcgactttggctgctgcggcaacgttgaagacggcttcgacggtttcggcggcattgggattttcctgtatccaccggtcaacggcttcgcgcgtattcttttcaaagcccgccacgctgcccaagccgccgatgacggcgaatttgccctcggcgggcaagggggcgatgttgcgcattgcggctttgtctatggcatagcgcgttccgtacagtatgtcgcctatgcccaaggcttcgcccgcgctgataaaggggttgagcgcgccggcggcgacgccgttgataaactccatgctgttgccccagcggtcgagcttggcattgtgctcgaacatttttctgttggcttcatcggcgcggtcggagaaattgctgccgaggttgctgtaattgtcggatatgcgttgccggatgctgcgggtgtcggtcggattgagtttgatactgcgggctgtgccgttgacgtgataggtgtattcgtctcgtgcgcccgtaggtttggggtaattgccgcccttcgggccgtcgtaggcatcggcgggatgatgttcgtgtccttcccagttgagccggtatacggtaaagccttcgtcaacgttgcctttttcttcgctcgcgctgtcggcggcgtggttgtcgaagggggcgtgttcttcgtgtccgtgtccggaaaagcgggtgtggtagccgattgtgccgttgatgtttgcctgttggatgagcaggttgcccatctggtgggtatagtcttggatgacgttgattttgccggtgcggtcggaaacgctgccgcgcgggtcgccgaagaggtggtatttgccgccgggttcgtagtgctgccgttgggcgttatcggtaatgaacgggtcttgcgccaagtccgccgcgagggcgggctgtatgagtgcggccgccgctacggcgcaggcggcaaggaggtttgtcagtctgcgcagcggtttcacggtttatcctcctttgcggcggcggatgacttcgttgccgacatcgggttttttaccgttgttttgtttgaagtcgggacggttttgggcggttgtgtcgccgtagggggtaatgtcggagaaatcgaccatcaggcggtctgaggctttgacggttttgctgactttgtaagggccggtccaaagggcgtattgttcttggtattgggattcgtaggcggcggttttaggggtaatcagcagtttccggctgtcgcggtcaacggcgaaatattcgagcttggtttgggctttaagggtttcggcgttgtagaggtgcagttcggtacggctgcggacggtgccgaatacgtcgacggttacgaatacgtcggtgtcggcgtattcgggcggtacgacttcgatgccgcgcaggtagaagacggtttggatgaggttggtcaggaaggaaacgtcgcgggggttggcgagcagggtttcgttgcggtagtcgcccgtgccgttgacggacagtccggcggagcgttcgcctttgcgtccgctgtttttcgtcagggcggcggcgggggcgttcaaaagcgatgtggaagtggttacgctggagagcgcgtcggatttggtggtggcggtagtgtcgtaggcggggtagctgtattgggtggcactttcggggttgttgtggtagccgccgcgtatcagtgcgtcgatagagtagcgtccgccgcttatgttgcccgaaccttggtcgcccataacggagacgtaaagggcggctttgcgtccttttagggcggacaaatccatttctttgacggcggcgcgggacgatgcggcgacgagttcttgttcgacggcaaagcgtttgccgccgccgtgggcgggtatgccggtcagtgtgccgcaggctgtgaggacgagggggatgaggaggagcagggttttcatagcggggtttgtttgatgttgaacggattttgagtgtaaagggattttaagggtttgtaaacaaaaggggcgaaaatgccgtctgagcggcggaaatggctttcagacggcatttgcgctcaataataatatcccgcgcccagaatacacggtttggatgcgccggttgctttgtgcggactaccgggaatgcgattaatccaacacgccgccaaccacgcaaatgcggcggcttccacccattgcggatcgaggttcaggtcggcggtgctgtgcagggaaacgcgtgtgccgaaacattctgccaaatccgccattaaaacaggattgcggatgccgccgccgcaaatgtacatttgacgggcatctgccgctgcgtgtgagacggcgtcgcaaacggtttgcgcggtaaaacgggaaagcgtccgcaatacgtcgtatcggttttcgccgccgtcaaggtaggtttcgagccaatttagggcaaacagttcgcgccccgtgcttttagggtggggttgtgcgaaatacgggtgggcgagcagcctgtcgagcagttgcggcaatatgttgccttgtgccgcctttgcaccgtttttgtcgtaaggaagctgccagtgtgcctgcgtccacg

附录Bcgtccatcagcatattgcccggccctgtgtcgaagccgaaggcgggtgcgtcgggggggagtacgctgatgttggcaatcccgccgatgttcagtaccgcgcgtgtttccctgttgtcgcggaacagggcttcgtgaaaggcggggacgagtggcgcgccttgtccgccggccgcaaggtcgcggctgcggaagtcgccgacggtaaaaatccgcgtccgttccgccagcagcggcaaatcggcaagctgtatgctgtaaccgtgttccggcgcgtgtcggacggtttgcccgtggcagccgagggcggtaatgtcggacggtgcgaggttttgactgcacagcagttcggcggcggtttgcgcatataggcggctgagttcttgcgacaaaatcctgctgcggtgcagttcgtctgcgcctgtgtcctgcaaatccagcaattggcggcgtaacctgccggggtagggggtaaaggcgtgcccttccgcgcccagccatttgccgccgtccatccgtatcagtacggcatccgccccgtccatgctggttcccgacatgatgccgatgtaaagctgtgtttccatcatcactcccaaactggtgcaaaacgccattttaacgtgtattgacgctcgtataccgatttgccgccgcagtgtaaataaagtgtaaataaatgtttcaagacggatggaaaaatattataatgcgcccgcaacatccagtagtagaagtgtcatacaaaccgtttccggcagcagttttgcattcggtcaggtttgggggtattcggatgcggttaggaaggatgcgtctgccatatcccgaaacggcagttcgaccggaggcagcagtacagtgtcggcaacactcatgatttccaccacattaaaggaagattgccatggctcaaatccaaatgagcgcaaatgttaaaaccatcaacgccgtctttgccgccatgctggtaggtacagtcggctattttatttattggggcttgggttatacccattacaattacgccgccttattcattattgccacgatgttcggcgtgtttatggcgttcaacatcggcggcaacgatgttgccaattctttcggcaccagcgtcggtgcgggtacgctgaccatcccgcaggctttgctgattgcggcggtatttgaggtcagcggcgcggtcatcgcgggcggcgaggtaaccaataccatacgcaaaggcatcgtcgatttgaagggtgttgatttcgaacccatacagtttgtgtttattatgatgtccgcgcttttggcggcggcgttgtggctgttgtttgcctcgaaaaaagggcttccggtatctaccacccattccattatcggcggcattgtcggcagcgcggtatgtatggcggtaatgaacgatgccgcatcgggcgatttgatacgttggggcaagctgggcggtattggtgtttcttgggtattgtcgcccgtgttgggcggcgcggtgtcctattttctgttttcgcgcgtcaagaaaaacgtcttagattacaacgcttgggcggaaggcacgctcaagggcatcaagcaggaaaaaaaggcctataaagaacggcaccgcctgtttttcgagggtttgtccgaagccgaaaaagtcgagtacgccaccaaaatggcgcacgacgcgcaaatttacgacgaacccgaattcgatccgcaagagctgcaatcggagtattaccgcggtctttatgcgttcgacaaccgtaaaaacaatgtcgattcctacaaggcactgcattcttggattccctttatcgcttcgttcggcgcgatgatgatttccgctatgctgattttcaagggcttgaaaaacctgcatttggggatgagcaacgtcaacagcttcctgaccatctttatgataggcgcggcggtgtggatggggacgtttgtttttgccaaaagcctcaagcgtaaagacttgggcaaatcgacctttcagatgttttcatggatgcaggtctttaccgcctgcggcttcgcattcagccacggtgcgaacgatatcgccaacgccatcggtccgtttgccgcgattatggatgttttgcgtaccaacagcgttgccgcgcaaaatgtcgtccccccgattgcgatgctgactttcggcatcgcgctgattgtcggtttgtggtttgtcggtaaagaggtgattaaaaccgtcggtacgagtttggcggaaatgcatcctgcttcgggttttaccgccgaactgtccgccgcctccgtcgtgatgggcgcgtcgctgatggggctgcccgtgtccagtacgcatatcttggtcggcgcggtactcggtatcggtctggtcaaccgcaatgccaactggaaactgatgaagcccatcggtttggcgtgggtcattaccctgcctgccgccgccgtattgtcggttgtctgctacttggttttacaggcagtattctgattgtaaaatactgatgccgtctgaacccgtgttcagacggcattttgttgatggaatgtgcgggcttgtgccttatgcacaatctgttctgtcgggatatgccgtttggtatagtgattaacaaaaatcaggacaaggcgacgaagccgcagacagtacagctagtacggcaaggcgaggcaacgctgtactggtttttgttaatccactatatcttggtttcggaacggtcggacacaaaggtgcggaacgttatgatatgccgccgcctgttcttgaaaacacttatcctgccggcagcaaaatgccgtctgaaaaagcctttcagacggcatttgtacgttagccacaatcacactgtttgcgaatatttcgccttggtttctttatggcgcaggtggtaatcgaagaccatggcgatgttgcggatgaggaagcgtcctttcggggtaacggtcagcccgtggctgttcaggcgcaccaatcccaaaccggcgagtttttccaaatccgccagttcgtctttgaagtagcggtcgaacgggatgccgaacatactttcgtaaatccgatagtcgagcgcgaaacggcacatcaaatcctgaatgatgttgcggcgcaggatgtcgtcctgattgagctggtagccgcgcatgatgggcagtctgccttcgtcgatggcggcatagtaggcatcgatgtcgcgttcgttttgggaataggtgctgccgattttgccgatggacgacacgccgatggcgaccaaatcgcaatccgcgtaggtcgaatagccttggaagttgcgctggaggaagccttctttgagggcgatggagagttcgtcgtcaggtttggcgaaatgatccatgccgatgaagacgtagccgcgttcggttagggtttggacgcagtattgcagcatatcgagcttctcttcgctgtcgggaacggcggcggtatcgatgcggcgttgcggtttgaacacgtgcggcaggtgggcgtagtgataaagggcgaggcggtcgggatcgagcgacaaaacggtatcgatggtggttttgatgctttccgaagtctggtgcggcaggccgtaaatcaaatcgacgctgacggatttgaaccccgcttcgcgcgccgcatcgatgacttctttggtttcttcgtaactttggatgcggttgaccgccgcctgcactttggggtcgaaatcctgaatgccgatgctcatgcggttgaagccgagtctgccgagcatgaggacggtgtcgcggctgactttgcgcgggtcgatttcgatggagtattcgccggtggggattaactcgaaatgtttgcgtatcatgcggaagacacgttcgatctgttcgtcgctcaaaaaggtcggcgtgccgccgccgaagtgcagttgggcaagctggtgccgtccgttcagatgtggagcgagcagttccatttctttttcaagatattcgatgtaggcatcggcgcggcttttgtctttggtgatgattttgttgcagccgcagtagtagcagatggtgttgcagaacggaatgtgaatgtaaagggaaagcggtttgtttaacgcgcccataccgcgcaaatgtaaagctttgatatattcgccttcgcggaaaccgtcatggaaacggtcggcggtagggtaggaagtgtagcgcgggccgctggcgggcaggctggcaatcagcgcgcggtcaaactcggggcggtcatcgtttacattgtgattgttctgtatctgaatgattttcatggtgtgtgtgtgcggttttatgatgttagtcaaattttggatagtttggtagaatgccacagtatgataaacctgtcttgatatgtgtcaataagcacatatagtggattaaatttaaataaggacaaggcgaggcaacgccgtactggtttaaatttaatccactataatcatgatggggcaaagcgcacaaaaaggtacggtatggcttcgcataatactacacatcagatgaaaacgctgtgttcttcctgttctttgcgggaactctgcctgcctgtcgggctgctgcccaacgagctcagccaactcgatgccgtcatccgtcaaagccgccgcctgaaaaagggcgaatacctgttctgtgtcggcgaagcctttacctcgctctttgccatccgttcgggcttcttcaaaacaaccgtcgccagtcaggacggccgcgatcaggtaacgggtttctttatgtcgggcgaactcatcggcatggacggcatctgttcccatgtgcacagttgcgacgcggtcgccttggaagacagcgaagtgtgcgaactgccgtttacccacatcgaagaactggggcaaaacatccccagcctgcgtacgcacttcttccgcatgatgagccgtgaaatcgtgcgcgaccaaggtgttatgctgctgttgggcaatatgcgcgccgaagagcggattgccgccttcctgctgaacctttcccaacgcctttattcccgaggttttgctgccaacgacttcatcttaagaatgtcccgcgaagaaatcggcagttatctcgggctgaaacttgaaaccgtcagccgcacattatctaaatttcatcaggaaggattgatttccgtcgagcataagcacatcaaaatcctcaatctgcaggtgttgaaaaaaatggtgtccggctgctcgcacgccatttgattaacccgtacgaacatttcagacagcattctcaataaacacagggcagacgaaaacatctgtcctgtttgttgtatctgccgcaaagtgccgtctgaaaaccggcagccgcctaaatcgaaaaatcctcgctgatgggcgtgtacagaatcctatccaccttctcgcgtgtcaggtgcggcgcgaacgcttggataaagtcgtaggcatatccgcgcaaataagtatcgctgcgcaaagcaatccacgtcggcgacggctcgaacaggtgtgccgcatccacaagctgcaaatcgccgtccgtatccgggttgtacgccattttcgccatcagtcccacgcccaaacccaagcgcacataagtcttcaatacgtccgtatctgccgcagccaatgcgacatcgggttgttccaaacgggctttggaaaatgcccgcgcgatgctgctgcccgcattgaatgcaaattcataagtaatcagcggaaacctcgccaaatcttcaatacggagggggtttctgcattcgagcaaggggtggtcgttcggtacgataaccgcatgagtccagtcatagcagggaagttttcccagttcgggatggtcgtctatccgttccgtaacaatcgccaagtccgcctcgcctgaggtaaccatacgtgcgatggcggcagggctcccctgtttgatggtcaggttgactttcggatagcgtttcacaaaatcggcaacaatcaagggtagggcatagcgtgcctgagtatgcgtcgtggcaaccgtcagcgaaccgctgtcctgtccggtaaactcgctgccgatatttttaatgttctgaacatcgcgcaaaatacgttccgcaatatccaaaaccaccttgcccggctgcgagaccgaaaccacgcgcttgccgctgcggataaaaatctgaatgccgatttcttcttccagcaatttgatttgtttggagatgccgggttgcgaagtaaacaaggcttcggccgcttcggaaacgttcaggttgtgctggtaaacttctaaggcgtatttcaattgttgtaatttcatggcgggtcggtgtgggtctgtgtcgggtggctgaacattgtttataatttatcatattttcttgccggtacggtatggggctttgccgttgtgtttgttgtttttgtgcaacggcaatcgtgcgatatggaaaaaatccccctaaagtaatgacacggaattgatttttcggcatgatagactatcaggaaacaggctgttttacggttgttttcaggcgttgagtattgacagtccgccccctgcttctttatagtggagactgaaatatccgatttgccgccatgtttctacagcggcctgtatgttggcaattcagcagttgcttctgtatctgctgtacaaatttaatgagggaataaaatgaccaaacagctgaaattaagcgcattattcgttgcat

附录Btgctcgcttccggcactgctgttgcgggcgaggcgtccgttcagggttacaccgtaagcggccagtcgaacgaaatcgtacgcaacaactatggcgaatgctggaaaaacgcctactttgataaagcaagccaaggtcgcgtagaatgcggcgatgcggttgctgcccccgaacccgagccagaacccgaacccgcacccgcgcctgtcgtcgttgtggagcaggctccgcaatatgttgatgaaaccatttccctgtctgccaaaaccctgttcggtttcgataaggattcattgcgcgccgaagctcaagacaacctgaaagtattggcgcaacgcctgagtcgaaccaatgtccaatctgtccgcgtcgaaggccataccgactttatgggttctgacaaatacaatcaggccctgtccgaacgccgcgcatacgtagtggcaaacaacctggtcagcaacggcgtacctgtttctagaatttctgctgtcggcttgggcgaatctcaagcgcaaatgactcaagtttgtgaagccgaagttgccaaactgggtgcgaaagtctctaaagccaaaaaacgtgaggctctgattgcatgtatcgaacctgaccgccgtgtggatgtgaaaatccgcagcatcgtaacccgtcaggttgtgccggcacacaatcatcaccaacactaaggctaggcaatatcttgccgatgcatgaggttagtggattttgtaccaggtactgttgcaatattcgtgaaacgtcggtcggcatcgatgatgtgaaacaaacccccgcttttgcggggtttgtttttttgggtggttttctgaaacggctatcgtcagaatcggggtgcaggttcggattcggattcagattcagattcagattcagattcagattcaggtttgtgtcccattgccgcgctttatagtggattaacaaaaatcaggacaaggcgacgaagccgcagacagtacaaatagtacggaaccgattcacttggtgcttcagcaccttagagaatcgttctctttgagctaaggtgaggcaacgctgtactggtttaaatttaatccactatatcggttgaaactctgattttaaggcggtaggatgtgggtttgcccatagaaagggaatcctttctgtatcaagccctgaaagggataattcatacaaattcacgcctttccccctcattgggaaatggatggaatcgtgccagatgtgtgcggcactgtatgccggatatggttttatcatcagcccttttcggttgaaaccccgtcagttgcagcgattgagcctaatcggtggcggaagttgccgctttgcattcggggcggcgtgcagtgcggtgctttgatatgccgtttgtgtgttgaaacagggtggtcggtgcatacgggtacggtatggccaaagctaaaagtgaaatacgctgaaacactgaatgagccgctttattgtttgtacggcctttgctgccttgctatgatttaaattggattcgcccgccggatattttgggatatgaaagaatttgacttcatcaaacggtatttgcaaacaggcacggataatgatgtcgtattgggcataggcgacgatgcggcgattgtccgcccgcgtgaaggcttcgatttgtgtttcagtgcggatatgcttttgaaggacaggcatttttttgcagatgtcaaacctgaagacttggcttggaaggttttggccgtcaatatttcagatatggcggcgatgggtgcgataccgcgttgggtgttgctgagcgcggctttgcccgaattggatgaggtatggctgaaacggttttgcggcagctttttcggtttggcaaaaaagtttggcgtaacgttaatcggcggcgatacgaccaagggcgatatggcgttcaatgtaaccattatcggcgaattgccgaagggtagggcgttgcggcgtgatgcggcggttgcgggcgacgatatttgggtgtcggggcgtatcggtatggcggcggcggctttgaactgccgtctgaaacggtgtgtgttgccagatgaagtgtttgccgaatgcgaacaaaagctgctccatcctgaaccaagggttgggctggggcttgcgctgttgccgtttgccagggcggcgcaggatgtttcagacggcctcgcgcaagatttggggcatatcctgaccgcttctggcaagggtgcggaaatttgggccgattcgctgccgtctttatccgtattgaaagatattttgccccgagcgcaatggctgtcttatactttggcgggcggcgacgattacgagctggtgtttaccgcgccggaaagttgccgcagccgcgtatttgatgcggcggaacggtgcggcgtgccggtaacgcgcatcggcaaaatcaacggaggatgccgtctgaaggttttagatgccgacggcagggaattggaactacattctttaggattcgatcattttggctgattttaaacctgactttgcgtggctgttgaaacggccgttgtgttttttggctttcggtttcggcagcgggctggctccgttcgcgccgggcacattcggcactttggcggcactgcctttggcgtttgtgctgattttgctcggcatagacgggctactgctggcttttttgtgtatcgtgctgtttatgtggggcatacgcatttgcgcttatgcggaacgtgaaacgggtgtcagcgaccacggtgggattgtttgggacgagattgtcgccatgctgtttgtgctggcgtttgtgccgttcaggtggacgtggtggctggcggcatttgtcctattccgtctgtttgacgcgctcaaaccgtctcccgtcggttggtttgacaagaatctgcacggcggtttgggcattatggcggacgatatggcggctgcggtgatgactttgattgtcttgaggattgcaatgctgttttaaacggtgctgccttgtaaaaatgccgcctgaaagcctttcagacggcattgtttcggaggttaacgcgttaccggtttgtatttgatgcgtttcggtttcgcgccttcttcgcccaaacggcgtttcttgtcggcttcgtattcctgatagttgccgtcgaagaacacccatttagagtcgccttcacacgccaagatatgcgtggcgatgcggtcgaggaaccaacggtcgtgcgaaatcaccatcacgctgccggcaaattccaacaatgcgtcttccaacgcgcgcagggtttccacgtcaaggtcgttagacggttcatccagcagcaatacattgccgccgctcaacaaggtttttgccaagtgcagacgaccgcgttcgccgccagacaattgacctgcaattttgctttggtcgctgcctttgaagttgaaacgccccaaatattggcgggcgggaatttcaaactgaccaacctgcaaaatgtcgcggccttcggcaatgttgtcgaacacggttttgtcgttttgcaaaccttcgcggctttggtcaatcaagctcattttcacggtttgtccgattttcacctcgccggaatcaggctgctctttgcccgaaatcattttgaacagcgtagatttacccgcgccgttcgggccgatgatgccgacaatcgcgcccgcaggcactttgaagctcaaatcgtcaatcagcactttatcgccgaacgatttggaaacatttacaaattcaatcacttcgttacccaaacgctcggcaacgggaataaagatttcctgcgtttcattgcgtttttggtattcgtagttgctcatttcttcaaaacgagccaaacgcgctttggacttggcttggcggcctttggcattttggcgcacccattccaattcctgcttcatcgccttcacgcgcgcggcttcggattttgcctcgttttccaagcgtttttctttctgctccagccaagacgagtaattgcctttccacggaataccatggccgcggtcgagttccaaaatccattcggcggcgttgtcgaggaagtagcggtcgtgcgttaccgcaacgactgtgccggggaagcgcacgagaaattgctccagccactcgaccgattccgcatccaagtggttggtcggctcgtccagcaaaagcatatcgggcttgctcaacaagagtttgcacaaggcaacgcggcgtttttcaccgccggacaaattatcgattttggcatcccattccggcaggcgcagcgcgtcggcggcgatttccaattcgtgttccgcaccgccgcccgtggacgaacctgccgcaataatcgcttccaagcggccctgctcttctgccaacgcgtcaaaatccgcatcaggattggcgtactcggcatacacttcttccaaacgtttctgcgcggcagccacttcgcccaaaccgctttccacttcctcgcgcacggttttttccggatcaagctcaggctcttgcggcaggtagccgattttgatgccgcccatcggcacggcttcgccctcaaattccttatccacgcccgccataatccgcagcacggtggacttgcccgcgccgttcaaaccgagcaggccgattttcgcgccggggaagaaagaaagggaaatatctttaatgatggttttctgcggcggcacaaccttgctcacgcgcagcatagaatagacgtattgttgggacatggttttctcgttttcatcaaacaaatttcagacggccattttaaccgataatttgatttaagccagtttatccgcgaaccggtattgccaaaatcgggcaggattcataaaatccgcttatccctttgaaattatatagacaaaaaaataataatgataggggatcgccgccccggcaaccatttcggattttccaaagcaaatatagtggattaacaaaaatcaggacaaggcgacgaagccgcagacagtacagatagtacggaaccgattcacttggtgcttcagcaccttagagaatcgttctctttgagctaaggcgaggcaacgccgtactggtttttgttaatctactatacttttcaaatcaaaaaaggatttaccttatgtcggaatatacgcctcaaacagcaaaacaaggtttgcccgcgctggcaaaaagcacgatttggatgctcagtttcggctttctcggcgttcagacggcctttaccctgcaaagctcgcaaatgagccgcatttttcaaacgctaggcgcagacccgcacaatttgggctggtttttcatcctgccgccgctggcggggatgctggtgcagccgattgtcggccattactccgaccgcacttggaagccgcgtttgggcggccgccgtctgccgtatctgctttatggcacgctgattgcggttattgtgatgattttgatgccgaactcgggcagcttcggtttcggctatgcgtcgctggcggctttgtcgttcggcgcgctgatgattgcgctgttagacgtgtcgtcaaatatggcgatgcagccgtttaagatgatggtcggcgacatggtcaacgaggagcagaaaggctacgcctacgggattcaaagtttcttagcaaatacgggcgcggtcgtggcggcgattctgccgtttgtgtttgcgtatatcggtttggcgaacaccgccgagaaaggcgttgtgccgcagaccgtggtcgtggcgttttatgtgggtgcggcgttgctggtgattaccagcgcgttcacgattttcaaagtgaaggaatacgatccggaaacctacgcccgttaccacggcatcgatgtcgccgcgaatcaggaaaaagccaactggatcgaactcttgaaaaccgcgcctaaggcgttttggacggttactttggtgcaattcttctgctggttcgccttccaatatatgtggacttactcggcaggcgcgattgcggaaaacgtctggcacaccaccgatgcgtcttccgtaggttatcaggaggcgggtaactggtacggcgttttggcggcggtgcagtcggttgcggcggtgatttgttcgtttgtattggcgaaagtgccgaataaataccataaggcgggttatttcggctgtttggctttgggcgcgctcggctttttctccgttttcttcatcggcaaccaatacgcgctggtgttgtcttataccttaatcggcatcgcttgggcgggcattatcacttatccgctgacgattgtgaccaacgccttgtcgggcaagcatatgggcacttacttgggcttgtttaacggctctatctgtatgcctcaaatcgtcgcttcgctgttgagtttcgtgcttttccctatgctgggcggcttgcaggccactatgttcttggtagggggcgtcgtcctgctgctgggcgcgttttccgtgttcctgattaaagaaacacacggcggggtttgagcgatgagcgatacccccgctacccgcgatttcggtctgatcgacgggcgtgccgtaaccggctatgtgctgtccaaccggcgtggtacgcgtgtctgcgtgctggacttgggcgggattgtgcaggaattttccgttttggcagacggcgtgcgcgaaaacctcgtggtgtcgttcgatgatgcggcttcctatgcggacaatccgt

附录Bttcagattaacaaacagatagggcgcgtggccggacgcatccgcggtgcggcgttcgacatcaacggcaggacttaccgcgtggaggccaacgaaggcaggaacgcgctgcacggcggttcgcacgggctggccgttacccgtttcaacgcggtggcggcagacggccgttcggtggtgctgcgcagccgcctgcaacagtcggccgacggttatcccaacgatttggatttggatatttcctaccgcttggacgaggacgaccggcttaccgttagctatcgcgccaccgcgctcggcgacacggtgttcgacccgacgctgcacatttactggcggctggacgcgggcctgcacgatgcggttctgcatattccgcagggcggacatatgccggccgatgccgaaaaactgcccgtctcaacggtttcagacgacctcgaagtatttgatttcagccggcccaagccgctggatgccgccgttgccgccctgcgccgcgaaacgggtcgggccggttttgacgacgcttaccgcgtgccgtccgatataggccgtcccgccgctgtgttgcaagccggacgccgccgtcgtatcagcatatacagcgaccgcaatggcttggtcatctttaccgccgccccgcaggatttcgcgcggcacgatgcgggcgtttacgacgcgctggcgaccgaggcgcagacgctgcccgacagcctgaattggcccgagttcggcaatattcgtctgaacaagggtgataccagggaggcgacgattgcttacggcatcgaatccctttcttaggagcttcctaacaccggttgcagacgacctttttatagtggattaacaaaaaccggtacggcgttgcctcggcttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttccgtactatttgtactgtctgcggcttcgtcgccttgtcctgatttttgttaatccactataagatttcaccattccctcaaatcaatccaaacaggagcttcataaatgtacacaagaatcatggaaatcagcccttggacgctgcgttcggcaaaactggaaaaagaacacaaacggctgcaagagagcctgaccagcttgggcaacggctatatgggtatgcgcggcagctttgaggaaacctattccgccgacagccacttaggcacctacatcgccggcgtgtggttccccgacaaaacccgcgtcggctggtggaaaaacggctatcccaaatatttcggcaaagccatcaacgcgttcaatttcagcaaagtcaaaatctttgtcgacgggcaggaagtggacttggcgaaaaacgacgttgctggcttctccgtcgaactcgatatgcagcacggcgtgttgcgccgctcgttcaccgtattcggtgtgcgtttcaatgtgtgcaaattcctgtctgtcgcacaaaaagagctggcggtcatccgctgggaagccgtatccgttgacggtaaaacccaccaagtccgcatcgattccatcatcgatgccgacgtgaaaaacgaagactccaactacgaagaaaaattctggcaggtattggacaaaggcgtttcagacagtctctcctacattgccgcccaaaccgtcgccaatcccttcggcgtggaacaattcatcgtcaacgccgagcaaacctttgccggcagcttcaaagccctcggcggcagccaaaccgactggcaggtctccaattcttttgaatccgaagtcggcagcacacccgaaacctttgaaaaacgcgtgattgttaccaccagccgcgattatcagagcttggaagcagtgaaagccgcaggccgcgccttgtcggaaaaaattgcaggcgttgcgtttgaaaccttgctggacgcgcacaaagcaggctggctgcaccgttgggaaatcgccgacgtggtcatcgaaggcagcgacgaagcgcagcagggcatccgcttcaacctgttccaactgttctccacctactacggcgaagacgcgcgactgaacatcggcccgaaaggctttaccggcgaaaaatacggcggcgcgacctattgggacaccgaagcctacgccgtaccgctctacctcgcactggccgaacccgaagttacccgcaacctgctgcaataccgccgcaaccaactgccgcaggcgcagcacaacgcgcgcgaacagggcttggcgggcgcactctatccgatggtaacgtttacgggcatcgagtgccacaacgaatgggaaatcaccttcgaggaaatccaccgcaacggcgcgattccttacgccatctacaactacaccaactacaccggcgacgagggctatcttgccaaagaaggcttggaagttttggtcgaagtgtcccgcttctgggcggaccgcgtccacttctccaaacgcaacggcaaatacatgattcacggcgtaaccggtccgaacgaatacgaaaacaacatcaacaacaactggtacaccaacaccctcgccgcatgggtattggactacacccgcgaagccttggcgaaatacccgcgtccggatttgaacgtgcgtgccgacgagttggaaaaatgggcggacatcagcgcgaatatgtaccgtccgcatgacgaagaactcggcgtattcgtgcagcacgacggcttcctcgacaaagacatccgccccgtgtccgcgctttcgcccgacgatttgccgctcaaccaaaaatggtcgtgggacaaaatcctgcgttcgccctttatcaaacaggcggacgtattgcaaggcatctacttcttcagcgaccgtttcaatatcgacgaaaaacgccgcaacttcgacttctacgaaccgatgaccgtgcatgaaagctcgctgtcgccctgtattcactctattctcgccgccgaactgggcaaagaagaaaaagccgtggaaatgtaccagcgcaccgcccgcctggacttggacaactacaacaacgacaccgaagacggcctgcacatcacctccatgaccggctcgtggctcgccatcgtccaaggtttcgcccaaatgaaaacctggggcggcaaactcagcttcgcaccgttcctgccgagtgcgtggacaggctacgccttccacatcaactaccgcggccgtctgattaaagtcgccgtcggcaaagaaaacgtcgtcttcactctgctcaaaggcgagtcgctcgatttgcaggtgtacggcaaagacatcacgctcgacggcagccacaccgttgcgttggaaaaataaggagggcgcaaaatgactttcactgcagtcctatttgacctcgacggcgtcatcaccgacaccgccgaataccactaccgcgcatggaaaaagctcgccgaagaactgggcatcagcattgaccgcaagtttaacgagcagctcaaaggcgtgtcgcgcgacgattcgctcaaacgcatcctcgcgcacggcggcaaaaccgtcagcgaagccgagttcgccgaactgacccgccgtaaaaacgacaactacgtcgagatgattcaggcagtcaaacccgaagacgtgtatcccggcattttgcccctgctggaagcattgagggcaaacggcaaaaaaatcgcccttgcgtccgccagtaaaaacggcccgttcctgctggaacgcatggggctgacccacttcttcgacgccattgccgaccctgccgccgtcgcacattccaaacccgcccccgacatcttcctcgcagcagccgagggcgtagatgcggacatccgccaatgcatcggcattgaagacgccgccgccggcgtcgccgccatcaaagccgccggcgccttgcccatcggcgtgggcaaagccgaagacttgggcagcgacatcgcgctggtctccggcaccgccgagctgacctacgcctacctgcaaagcgtgtgggaacagtcgggcaggtaaaacgcgtcagataaagtgtcaaggaagcaaaagaccgtctgaacagtgtttcagacggcctttttgcttttagaacagaatgataacccaacttacgcaaccctaaaaactaaatgccaatctcttaaccatgctattcaaatttatttgaacgattttttttctaaccagccaaccttaacaatcactattaaaatgcgcgccgatgttctgtctccgcctgtatgcggcttgggcgacggcgaggctgcattcgagcaggttgcggttttcgtattcggacgcggtgtgcggttcggcttggttttgcttccaaagctgcagttgggcgatggcgcggcgcaggccggtatcgttgcgtaggatgcctagatggcgttggttgaacgtttgcaggacggggcggctgaatgtgttttgaaggtcgtctgaaaagatgcctgcttcggcggagaggctttcagacggcctttggaatggttcggcttggaatgcttgtccgtctgcgatggcttgggcgcagagccttgcggtcacgacgcattcgagcagggagttgctggcaaggcggttggctccgtgcagcccagtgcaggcggtttcgcccaaggcgtagagctgcggcagggaggttctgccgcaggggtcggtttggatgccgccgcaggtgtagtgttgcacggggcggacggggatggcttggcgcgtgatgtctaggccgcattgggataaacagtgtcgatggatggatgggaaatgccggcggacgaacgctgcgggttgatggctgatgtcgagcgagacgaagtcttgcgtttgtttggcgatttcggctgcgatggcgcgggcaacgatgtcgcgcggtgcgagttcggcgcggcggtcgtaatgcggcataaatcgttcgcccgcttggttggtcaggatgccgccttcgccgcgcacggcttcggaaatgaggaaggtgcgtccgttttcagacggtcttgccaagcctgtggggtggaattggataaattcgaggtttccaactgcgcagcctgcgcgtatcgccatggcgatggcgtcgcccgtgcattcgggcggcgtggtggtggcggcgtaaatctgtcccaagccgccgcctgcgagtacggtatggcgggcgcggatgcggtaggtttcttgtgttcggcagtcgaggacggtcagtccgcacgccgcgcctgattcggtttgaatgtccaacgccatctgccgctcgcaaacgcggatgttcgggcggcggcgtatttgggcaatcaggctctgcatgacggcttcgcccgtgtagtcggcgacgtgggcgattcgtcggcaggtatgcccgccttcacgcgtcaggtgcaggccgttatgattccggtcgaacgccacgccctgcgccagcagccattcgattgccggtttgccctgcgacaggatggcgcggacggcggcttcatcacacaaacccgcgcccgcttccaaagtatcggcaacgtgtttttcgatgtcgtcctctcccgaccacgccgccgcaatcccgccttgcgcatgacggctggcggtgtcgtccagccggtttttgcacaaaataacgatgcggaacgattcaggcagcgacagggcgagcgtcagtgccgccagcccgtttccggcaatcaatacgtcgcaatcggtttgcatggtgttgtccttgtttgagaggccgtctgaaacggtatagtggattaatcaatgccccgacatatgcgacatggtattgagaagcaccacgcccagcaaaatcaaaccgatgctgacaatcccaatgaaatcagctttctcaccgaaaaacaccacgctgactaaagccgttaaaaccagtcccacgcctgcccaaatggcgtatgctgtagccagcggcatggttttcagtgtcatagacaaggcccaaaaacacaccgaaaagctgactaccacgccaatagaaggccacagtttgctaaacccgccactcagtttgagcatggaagaaccgcagacttcgcttaaaattgctacagtcagaaagagccagtgcatttgcatgtttttacctgataaatgaaagaaagtataattatatcaatgcaataaaataaaaaaacagtcttgttgttaaagattttttgtgtgcaaatcccgtcttgggaaagcaggcgggcggtattttcaggctgcacccattacgaacgacaaatcaggcggggcccatgccgttgaacacatcttttttcttcagccctgccgcaaagtcgagcatacgctgcaaaggcagtttggcggcttcgcccagcttcctgtccaacaggatttcgttacgtccgcttgtcagggcgtatttgatgccgcccagcgaattcatcgccatccacgggcagaacgcgcagcttttacagcttccaccgttgcccgccgtcggcgcggcgataaattgtttgtcgggcgcctgcttttgcatttcgtgcaggatgcccaaatcggtcgccacgatgaattttttttcaggacgcgatacggcggctttgagcagtttgctggtcgagccgaccacgtcgcccagttcgatgacgctttgcggcgattcaggatgaaccagcaccaccgcttcggggtgttccgccttcaacgccgccagctcttgccctttgaattcgttgtga

附录Bacgatgcacgaaccctgccacaacagcatatccgcgcccgtttcgcggcagatgtagtcgccgaggtggcggtcgggtccccaaatcagcttctcgccgcgtgatttcaaatacgatacgatttctaacgccaccgaagacgttaccacccaatcggcacgcgctttcacggcggcggaagtgttggcgtacaccaccaccgtgcggtcggggtgttggtcgcaaaacgctgaaaacgcttcttccgggcaacccaaatccaaagaacattccgcctccaaatcaggcatcagcaccgttttttcagggcagaggattttcgcgctctcgcccatgaagcgcacaccagccaccaccagcgtaccggcttcgtgttccgcaccgaagcgcgccatttccagcgaatcgcccacgcatccgcccgtctccaaagccaaatcctgaatcagcggatcaacgtaataatgcgccaccaagaccgcgtttttctccttcagcaaagccttgatttcgtctttcagacgatctgccgtctcgcggtcgggcgtgtcggcaaccttcgcccacgcctgacggatttggcaggcggaagtcggcgtttggatgagtggcatatcgtagtcgaacgagcggcgggcggcggtttgcatgatgtttccttgtagctgtttttcagacggcatgaaggtttgccgtctgtttttcaaactgtttttacattatgctcaacttgagtataatatgcaaggtcgtctgaaaacaggtttgcaataccgtaaaaccgacccgcttcgttccgacaaaccgctttggtttacaataaagcctttcccacccgcagaaagccgagcatggatgcctaccccgaagccgaagccccgccgcaaagcatcgtcgagctggttcccgtattgattgccgttaccgacggcggcctgcgggtattgaccgtcgcccaaggcatgctcctgcccaacggcccgctctcccccctgcgcaattccttgcaggcaggcgtaaaactgtgggtcgccaagcagacttcgcagcctatgggctatgtggaacagctttacacctttgtcgatacccaccgccgcaacgaacacggcatgcccgtgctgtacgtcagctatttggggctggtgcgcgaggcagccgacagcatcctgcacccggatgcgaaatggcaggactgctacggctatttcccgtgggaagacttgcgcaccgacggcgggcagcgcgacgccgtcgtcggccgcctgcgcatttgggcaaactcggcggacacggaggaagtgcgccaaaagcggctcaagcgcattcatttgtgctggggggtcgaaccggaaaactggtcggaagaatacgttttgcaacgctatgaaatgctgtatgaaagcggcctgatagcggaagccgccgagccgcaggcaaacttcgacttcgcgcttacggggcagcccatgcgccacgaccaccgccgcgtactggcgaccgccctgtctcgcctgcgcgccaaaatcaaataccgccccgtgatttttgaactgatgccgcccgaattcacgctgctgcaactgcaaaacagcgtcgaagccatcagcggcagattgctgcacaagcaaaacttccgccgccagattcagcagcaaaacctcatcgagccgtcggataccggcgtatcgggcagcaaaggccgtcccgcgcagctttgccgcttccgcgacgacgtcctgcccgacaggctgatttcggacatcggactgccgctgggcagccgttagcccgttttcagacgacctatagtggattaacaaaaatcaggacaaggcgacgaagccgcagacagtacaaatagtacggaaccgattcacttggtgcttgagcaccttagagaatcgttctctttgagctaaggcgaggcaacgccgtaccggtttttgtaaaatgaagttttgccccatcggtgcaacatcaatctttttcaacaaaggaaaccccatgccgtctgaaaaaaccctctttcccctgcccgacaccctgttgcgccccatagtagaacaagccttgagcgaagacttgggcaggcgcggcgatattacgtccgccgccgtcatcgcccccgacaaaaccgccaaactcttccttgtcagccgcgaagacggcgttatcgccggcatggacttggcgcgtctcgcctttcagacgatggatccgtccgtccgcttccaagccgaaatccgagacgggcaagccgtccgcgcaggtcagacgcttgccgccgtcgaaggcaacgcccgcgcgctgctcgccgccgaacgcaccgcgctcaactacctcacgcacttaagcggcatcgccaccgccaccgcgcgtgccgttgccgaagtcgccgaatacggtacagacatcgtgtgcagccgcaaaaccatccccctgctgcgtgtcctgcaaaaatacgccgtcagggcaggcggcggtgtgaaccaccgcatgggtttggacgacgccgtgctcatcaaagacaaccacctcgcctattgcggcagcatcgcccaagccgtgcagcaggcaaaacaggctgtcggagcattgacctgcgtggaaatcgaagtggatacgttggcacaactggacgaagccatcgcagcgggcgcggaacggattttgctggataacatggacgacgaaaccctgaaagaagcggcaaaccgctgccacacgcaaaccgcccacccccacaccatctattgcgaagcatcgggcggcatcggcttcgaccgcctgaagcgcgtggcgcaaaccggagtggacggcatcgccctcggctatctgacccacagcagccgttcgttggacataggtttggatttcgtggcgtgagttttagggtgcgggcggctgtctgatatgtcaggcaaggaaccgcttaaccctaatccggttattgcctcagggaggaaatgccgtctgaaagattcttcagacggcatttttcgtaaaggtcgtgatgctttagaaaaaacagcatttcaggcaggtattttgtttgcccgacagcgcggcggcatcggtagggcaggaaaaaggacggggggcggcagttttatgccgtctgaaagcccgcctttacgcttgtttgcaaaaaaagtgggaaaaggaacatacaatcctgtacaatcatccataaatatttgatttataatacgatttataaagataatcacaatcatccatatctgccgcccgtcaatccgcttggcgggcggcaaaggttttaggaataccgatgaacacaataccgctccacaccatactcaaacttatggcgcatcccgaacgtatggcgatactgattcaattgttggacagcgaacgcaatatcgccgaactggcaaaatccttatccctgccggccaccgcagtttccaaccatttgaaccgcctgcgcgtggaaggtctagtcgattttacgcgttaccaccgcattatcgaataccgcctggtttccgaagaagcggcggcgattctgcacacggttcgcgatttggaaaacaaacgcgtggcatagtgttagaatcctttccttttgccgtctgaacgtttcagacagcatttttcggaaatgttatgaaaatcaccacttggaatgtcaattcgctcaatgtgcggctgccgcaggtgcaaaacctgcttgccgacaatccgcccgatattttggttttgcaggaactcaaactcgatcaggacaaatttccggccgccgctttgcaaatgatgggctggcactgtgtttggagcgggcagaaaacctacaacggcgtggcaatcgtcagccgcagcgtgccgcaggacgtgcatttcggtttgcccgcactgccggacgatccgcaacggcgcgtgattgcggcaaccgtcagcggcgtgcgcgtcatcaatgtctattgcgtcaacggcgaggctttggacagccccaaattcaaatataaggaacagtggtttgccgcactgacggagtttgtccgcgatgaaatgacccgccacggcaaactggtgttgctgggcgatttcaatatcgcgcctgccgatgcggactgttacgaccctgaaaaatggcacgaaaaaatccactgttcgtccgtcgaacggcagtggtttcaaaacctgctggatttgggactgaccgacagcctgcgccaagtccatcccgaaggcgcgttctatacctggttcgactatcgcggcgcgatgttccaacgcaaactgggcctgcgtatcgaccatattttggtgtcgcctgcgatggcggcggcgttgaaggatgtccgcgtcgatttggagacgcgcgcgctggagcgtccgagcgaccacgcgccggtgacggcagaattcgattggtaaaagaccgtgttttgatatggcgttgacaagcatccttatcttcaatttattcaataggatagctttctatctgactgaaaaataattgcctttccccggcaaacagccgaaatcggcggattgttcaaacacagcctattttcctgaaaaatttatgaaatacatagggttaatatcagattttggagcagtaaaatttattatgtacactaatccaaaacaaaatcaaatattgaaaactagatttattttcgaataaatagaaagccgtcttatatatagtaataaattaataaccctgtttttcctattgcctttattgtgccatgcagttgagtttgatgaaactcaatataacgactgtaaagataaatctatgttatgtgctgtcagaattgattctcccaaaggcaataactatagtggattaacaaaaatcaggacaaggcgacgaagccgcagacagtacaaatagtacggcaaggcgaggcaacgacgtactggtttaaatttaatccactatataaatctatgtggtttgacaatggcaagttagtatttatatcctttactaatcaacaaatggaaaatcaaagtcgcccatctctagcgatgtttattagtgatgacaaaatatccagtaccaatattgatgaatttttagcatctttcgatcctgataaatatcgaatatttcatgatccaagatataaatttttacctagtatgtcgaactcattgtaatccttattctctttttgatattgatagcaaatataaacctgatgagaaagataaaatctttttttcaatcccgacagataacacagatttttataagggtttttatttaaataaggattatatagaaggtatatatcctagtaggcataatggcagctattacaaaatatagtggattaaatttaaaccagtacagcgttgccgtactatttgtactgtctgcggcttcgtcgccttgtcctgatttttgttaatccactatatctgcatcagtttcatgaaacgcaagtcggaagcgtcaaacaactgattgcccattttgaccggctgattgacgaattggacaaacaaatcgacgaccacacccacacgcattttgacggcaaagcccaagtggcagaacaaatcaaaggcatcggttcgataacgacggctacgctgatggcgatgctgcccgaattgaggcggctgtcgcacaaacggatagcgggtttggccggcattgccccgcacccgagggagagcggggaaaccaaattcaaaagccgctgctttggcggaaggtctgcggtgcgtaaggcactgtatatggctaccgtggcagcgacacgttttgaaccgcttattcgggatttccaccaacgcccgctgtccgagggtaagccgtataaggttgccgttacggcatgtatgcgcaaactgctgacgatatcgaatgcccggatgcgtgattattttgccgaaaacgataccgccgaaaacggtatctaaacggcttgatttgagttttggtatttttgcccgacggggtgaaaaatacagttgctacggctcgatgaatcgtcagaaatacctgcatcgtcattcccgcgcaggtgggaatccagaccggtcggtgcggaaacttatcaggtaaaacggtttcttgagatttttcgtcttggattcccactttcgtgtgaatgacggaatgtaggttcgtgggaatgacgtggtgcaggtttccgtatggatggattcgtcattcccgcgcaggcgggaatctagtctgttcggtttcagttattttcgataaatgcctgttgcttttcatttctagattcccactttcgtgggaatgacgggattttaggtttctgattttggttttctgtccttgtgggaatgacgggatgtaggttcgtaggaatgacgtggtgcaggtttccgtgcggatggattcgtcattcctgcgcaggcgggaatccagtctgttcggtttcagttatttccgataaatgcctgttgcttttcatttctagattcccactttcgtgggaatgacggttcagttgctacggttactgtcaggtttcggttatgttggaatttcgggaaacttatgaatcgtcattcccgcgcaggcgggaatctggaatttcaatgcctcaagaatttatcggaaaaaacaaaacccttccgccgtcattcccacgaaagtgggaatctagaaatgaaaagcaacaggaa

附录Btttatcggaaatgaccgaaactgaacggactggattcccgcttttgcgggaatgacggcgacagggttgctgttatagtggatgaacaaaaaccagtacggcgttgcctcgccttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttccgtactatctgtactgtctgcggcttcgtcgccttgtcctgatttttgttaatccattataaaaatgccgtctgaaaggttttcagacggcattggttcacgggccgcgcccgggtatttcggcaaaatcagtcggcgaccgccatcaggctggcgttgccgccggcggctgtggtgttgacgctgcaagagatttcttcaaacacttgcaggatgtcgagtccgttttccgaagggaggatgcggatgagtgcgccgtcgtgggcggcaagttcctgtttgcgcgcgctgtccaaaggcgacagggcggcaacgtggctgatgccggcggtttcgggtttgccgttgaccagcagcagaccttccaagtcggcagtgtaggaagccaaggggctgtcgggttcgaccactgcctgtatgccggaggcggcaagttcggtcagtgcggcaaaggcttgaaccgtgctgccgccgtgtatccaaacgcgtttgggcgcgtgccatgagatgctgttgcgctcgccggtcggtccggtaaggacggtttcggcacggcgcagggtgcggatgcgggcgtgtcccaaagcggccgctgcggcttttttctcttcggcgttgaacggtagtttgtgaaccagtgcttcgaggcgtttgagtgcggcttcgtccgcctgtccgatttggctcagggtcggggcaacccattcgccggcgcgggtcagtttttgcaggtagaacgaaccgcctgctttggggcctgtgccggacagaccgtgtccgccgaagggctgtacgccgacgactgcgccgacgatgttgcggttgacgtaaacgttgccggcttcgatgcggctgcggatgtggcgtaccgtgccttcgatgcggctgtgtacgccgtgggtcagggcgtagcctttgctgttgatttggtcgatgacgttgtcgagttcgtcggcgcggtagcggacgacgtgcaggacgggaccgaagacttcgcgttgcagttcgttgaggttgttcaattcaaacaggatggggcgaacgaacgtggattttttggaatcgacatcggcggcggttttgacttcgtggtaggacttggcaacacctttcattttgttgatgtggttcaacaggttttgctgtgcttcggcatcgatgacggggccgacatcggtagtgagctgaatcggtttgccgacgacgagttcgtccatagcgcctttgatcatgtcgagcatacggtcggcaacgtcttcttggacgcacaaaatgcgcagggcggagcagcgttgtcccgcgctgtcgaaggcggagttcaatacgtcggcgcagacttgctcggcaagtgcggtggaatcgacaatcatggcgttttgtccgccggtttcggcaatcaggacgggattgtcgccgcgtttggcaagggctttgttgatcaggcgcgccacttcggtcgagccggtgaaaatcacgccgccgatgcgggcatcgttggtcaatgccgcacccacgtcgcctgcgccgaggacgagttgcagggcggaagtcgggatgccggcttcgtgcatgagggaaacggcataaccggcaatcaggctggtttgttcggcgggtttggcgatgacggtgttgcctgccgccaatgcggaaacgacttcgccggtaaagatggcgagcgggaagttccacgggctgatggcgacaatcgcgccgacggcttttgcgtcttgaggcagggtatgttcggcttcgtttgcgtagtagcggcagaaatcgacggcttcgcgcacttcggcaatggcgttgttcagcgttttgcctgcttcgcgcacggcaagcatcatcagtgctggggtgtgctgctccagcaaatcggcaaaacggcgcaggcaggcggcgcgttcggcggcaggtgtcgcactccattcggggaacgcggcaacggctgcgccaaccgcttcttgggcaagcgcggcatcggcaaagctgactgtgccgacgatgtcgtcgtggtcggcagggtttttaatcggttgcgcttcgccgacatcgcgggctttgccgttgacgatggatgcggcgtggaagtcttgcgcggcggctttgttcatctgttcttgaagctgctgcaatacgttttcgttgctcaagtccacgccttgcgagttcagacggcatttgccgtacaaatcgcgcggcagcggcagggcgttgtgcaggtggatgccttgttcggcgatggtgtcgaacgggctgcggatgagcgtgtcgatgctgatgttttcatcgacgatttggttgacgaaagacgagttcgcgccgttttccaacaggcggcgcaccaagtaggcgagcagggtttcgtgtgtgccgactggggcgtacacgcgcacgcggcggcctaagttttgcgggccgacgacttggtcgtacagggtttcgcccataccgtgcaggcattggtgttcaaaatctttgcctttacccatttggtagattgcgcccaaagtgtaggcgttgtgggtggcaaattgcgggaataccgcgtcttgcgcggaaagcagtttgcgcgcgcaggcgaggtaggagatgtcggtgtggactttgcgggtgtaggtcggatagccgttcaagccgtccacttgcgcccatttgatttcgctgtcccaatacgcgcctttgacgaggcggatcattagtttttggttgttgcggcgggcaaggtcgatcaggtagtcgataacgaacggacaacgtttttggtaggcttggacaacgaaaccgatacctttgtagccagccaagtcagggtctgaaaccaaagcctccatcaaatccaaagacagctccagacggttggcttcttcggcatcgatgttgataccgatatcgtattttttacccaaaaggaacagctctttcaggcgcggcaacagttcgcccatcacgcggccgtgttgggtgcgcgagtagcgcggatggatggcggaaagtttgacggaaataccgttaccttcgtaaacgccttgtcctgccgcatctttgccgatggcgtggatggcttcgacatagtcgcggtagtagcggtcggcatcggcttgggtgtaggcggcttcgcccaacatatcgaaggagaagcggtagcccattttttcgcgttctttgccgttttgcagggcttcttcaatggtctgtccggttacgaactgtttgcccagaagccgcatggcgtaatttacgccttggcggatgagcggtgcgccgcctttgctgatcaggcggctgagtgcggaactcatttgtttgtcgtttgtggcggtcagtttgccggtaatcagcaggccccaggcggcagcattgacgaagagggaagggctgttgttcaaatggcttttccagttgccgtctgaaatcttgtcggcaatcaggcggtcgcgcgtggcgttgtcggggatacgcagcagggcttctgccagacacatcagcgcgatgccttcttcgctggagagtgaaaactcgtgcatcagcgcatccacgccgccggctttggtgcggccggcgcggacttgggtaaccaaacggcgggcaagctcggaggcggcgttgcgctcttcgtcgctcatctgtgcacgttgcaacatatcctgtacggcttcgatttcattacggcggtaggcatcggttatcgcttggcgcagggcagtttgtgccggaaatgcaaaatgaaacattttttggattctccaaagtttttcggggggcaggcggcatcggtgcggcctgaatacggtaatatcgtaataaatccgcagatgaaatacaaggcttcaaatgcgggcagggtaggtgcttccgtttctttgaaaatgaaacgggtaaaacacaaataaggcctgtatgcaggcaaggtttatttgtgtttgacccggaaacgggttcagacggcacgaaccgggatgccgtgccgtctgaaaggggtttatcgggtggcgcggtaatctgcgtcggctttttcaaagcgttcttgggtttcgcgcgaaggttctttgttgaacagggaaaccaacacggcaacgatcaagcaaacaataaagcccggcacgatttcgtacatcgtcaacaagccgctttctcctgccgcttgagccggttttttcacccattccgcccatacgactacggttaacgcacctgcaaccatacccgacaacgcgccgtaggcagtgatgcgtttccacaatacggacagaatcacaatcgggccgaatgccgcgccgaaacctgcccacgcgtaagacaccagtcccaatactttgctgttcggatcggaagcaatcaggatggaaatcacggcaatcgccaagaccatcaggcggccgacccataccaattccgactgttgcgcgtttttacgcaaaaagcctttgtagaagtcttcggtaatcgcgctggagcaaaccaaaagctggcaggacagggtggacatcaccgccgccaaaatcgcgctcaaaataatgccggcaatccaagggttgaacagcagggtggaaagcgcgatgaagatgcgttcgtggttgccgctcatagaagaaactttgtcgggatttgcaccgaaatacgcaatgccgaaataaccgaccgctaccgcgcccgcaaggcacaacgccatccaagtcataccgatgcggcgtgcggataccagcgatttcgcgctttcggccgccataaagcgcgccaaaatgtgcggctgtccgaaatagcccaagccccatgcggcggtggaaatgatgccgatgacggtcgtaccggcaaacaggctgccgtattctttgcccgtgcctgcggcgacactttgaatcgcggcagacatctgttccgcgccgcccaagcccagatagaccatcacaggcgttaaaatcagcgcgaaaatcatcaaagaagcctgcagcgtatccgtccagcttaccgccaaaaagccgcccaagaaggtataggcgatggtcgcgcccgcgcccagccacattgcctgattgtaagtcataccttcaaacaggctttggaacagggttgcgcccgccacaatgcccgaggcgcaataaatcgtgaagaaaaacaggataatcagtgcggaaaccactttcatcaagtgtccgcccgcgccaaagcggtggaagaaataatccggcagcgtcagcgcgttgttggcgtattcggtatgtacgcgcagacggcccgccaccaaaagccagttgaaatacgcgccgaccaagaggccgatggcaatccaagcctcattcaaaccgctcaaataaatcgcgcccggcagacccatcaaaagccagccggacatatcggacgcgcctgccgacatcgcggtaacaaacgggcctaggctgcgcccgcccaaaatataatcgtcgaaattgcgcgtagaaaaataggcggcaagcccgatgagaaggactgcaaccagatagattgcaaaagtaatgtacatgggattcatgtgctattcctcgtctaaaacttcagaattacaggctttgaaattgcaagcaacttgcgcctgaaatgtttttctaataaaagtacaacggaaaatccggatacccgaaagggggattcggataaattatcttcaatcacaataagatatgtaataaaactatatgaaattgtaaataatccgtttcaggataacccaatttctgttgtttgcaaagcacttaatggcttaaaaagccgagtttgaaacgatgcgcgtcggaaaaatcatttaaaacagcatattgttttgtagtgtcttgtaatcgggcgttgcgcggaatatgaaatccgttttcaggcggcaggtgttttgaggtgtaatttagcaaccgcaaaggaggcgcggtatgttttgccgattatccgccgcccgttttcagacggcatttttccttatacaatagccgattgaatttgatatgttcaggaaggatacagattatgttcggcaagcagctttttgaggaagtcggctcgaaaatcagcgaaaccatcgccaacagccctgccaaagatgtggaaaaaaatattaaggcgatgctgggcggcgcgttcaaccgtatggatctggttacgcgcgaagaattcgacatccagcagcaggttttaatcaaaacccgtaccaaactggcggctttggaagcgcgtttggaaaaactcgaagccgcgcaaaatcccgaacgggcagcattggaagcggctgaagccgctgccgaagaagccgtcgccgaaatcaggcagcaaaccgaagccggcgaataaggtcgtctgaaatatgtcgcttgccttggtttacagccgcgccttgagcggtatgaatgcgccgttggtcgaagtggaagcccaccttgccaacggcctgccacatttcaacatcgtcggactgcccgatatggaagtaaaggaaagtcgcgaccgtgtccgtgccgccattattcaaagc

附录Bggttttgaattccccgccaaaaaaattaccgtcaacctcgcccccgccgacctgcccaaagagtcggggcgtttcgatttgccgattgcaatcggcatccttgccgcatcggggcaggttgcgcccgaaaaactggaggaatacgagtttgcgggggaattggcactgtcggggctgttgcgccccgtgcgtggcgcgttggcgatggcgtggcagggtatgcaggcaaaacgtgcatttgttttgcctgaagaaaatgcaggacaagccgccgtgatgcgcggcattaccgtttacggcgcgcgctctttgggcgaagtcgccgcccatttgaacggcatcgaacctttggcgcaaaccgaatgccaagttcctcagatgccgtttgaacatggcggacaacctgatttgtgcgatgtgaaaggtcagcacaccgcgcgccttgctttggaaatcgctgccgcaggcggacacagcctcttgatgatgggtccgccgggaacgggcaagtctatgctctcccaacggctgcccggcatcctgccgccgctgaccgaagacgaattggtagaagtttgggcattgcgttcgctcctgcccaaccaccaacaacaactcgacagcaaccgtcctttccgcagtccgcatcacagcgccagcgcggcggctatggtcggcggcggttcggatccgcgtccgggcgaaatttcattggcgcaccacggcgttttgtttttggacgagctgcccgagtttgaccgcaaagttttggaagttttgcgcgaaccgttggaaaacggcgaaatccacatttcccgcgcggcgcgccaagccgtctatcctgccaaattccaacttgttgccgccatgaacccctgcccgtgcggttatctcgggcatcccgtcaaaccctgccgctgcacgcccgaaagcgtcgcgcgttaccgcagcaagatttccgggccgctgctcgaccgcatcgatttgaccatcgaagtcccgagcctgtccgccgccgaactgatgcagcaggaagcaggggaaagcagcgcgtccgttttggaacgcgttatcgccgctagagacaaacaatacgcacggcaaggcaaagtgaatgccgccttgagtgtcagtgaactcgacacatccgcccgcattcaaaaagaagcgcaggaagcattgggcggcctgctggaaaaactctccctttccgcccgcagcttccaccgcattatgcgcgtggcgcgtacattggcggatttggcgggcgacgaagaagtcggcagaagccacgtcatgaaagccataggtttccgtcgtgctttataggaatgggaatggaagcaggttttgcccaaatatggcgatattgttagaatatccgcccgtaagcaaacggcgttaatgccgtctgaaacacattaaggtatgtttatgaacaaattttcccaatccggaaaaggtctgtccggttttttcttcggtttgatactggcgacggtcattattgccggtattttgttttatctgaaccagagcggtcaaaatgcgttcaaaatcccggcttcgtcgaagcagcctgcagaaacggaaatcctgaaaccgaaaaaccagcctaaggaagacatccaacctgaaccggccgatcaaaacgccttgtccgaaccggatgctgcgacagaggcagagcagtcggatgcggaaaaagctgccgacaagcagcccgttgccgataaagccgacgaggttgaagaaaaggcgggcgagccggaacgggaagagccggacggacaggcagtgcgtaagaaagcgctgacggaagagcgtgaacaaaccgtcagggaaaaagcgcagaagaaagatgccgaaacggttaaaaaacaagcggtaaaaccgtctaaagaaacagagaaaaaagcttcaaaagaagagaaaaaggcggcgaaggaaaaagttgcacccaaaccaaccccggaacaaatcctcaacagcggcagcatcgaaaaagcgcgcagtgccgccgccaaagaagtgcagaaaatgaaaacgtccgacaaggcggaagcaacgcattatctgcaaatgggcgcgtatgccgaccgtcagagcgcggaagggcagcgtgccaaactggcaatcttgggcatatcttccaaggtggtcggttatcaggcgggacataaaacgctttaccgggtgcaaagcggcaatatgtctgccgatgcggtgaaaaaaatgcaggacgagttgaaaaaacatgaagtcgccagcctgatccgttctatcgaaagcaaataattatgaagctcaaacatctgttgccgctgctgctgtcggcagtgttgtccgcgcaggcatatgccctgacggaaggggaagactatcttgtgttggataaacccattcctcaagaacagtcgggtaaaattgaggttttggaatttttcggctatttctgcgtacattgccatcatttcgatcctttgttattgaaactgggcaaggcattgccgtctgatgcctatttgaggacggagcacgtggtctggcagcctgaaatgctcggtttggctaggatggcggctgccgtcaatttgtcgggtttgaaatatcaggcaaaccctgctgtgtttaaagcagtttacgaacaaaaaatccgcttggaaaacaggtcggttgccggaaaatgggctttgtctcaaaaaggctttgacggcaaaaaactgatgcgcgcctatgattcccccgaagctgccgccgccgcattaaaaatgcagaaactgacggaacaataccgcatcgacagcacgccgaccgttattgtcggcggaaaataccgcgttatcttcaataacggctttgacggcggcgttcatacgattaaagaattggttgccaaagtcagggaagaacgcaagcgtcagacccctgctgtacagaaatagccgaactcccgtatccgaaagaagcgcaagcaatggattttctgattgtcctgaaagccctgatgatgggcttggtagaaggttttaccgaatttttaccgatttccagcaccggacatttgattgtgttcggcaatctgattggttttcacagcaatcacaaggtttttgaaattgccatccagctcggtgcagttttggcggtagtgtttgaataccggcaacgtttcagcaatgtgttgcacggcttgggaaaagaccggaaagccaaccgcttcgtccttaatcttgccattgcttttatacctgccgccgtgatggggctgttgttcggcaaacaaatcaaagagtatctgtttaaccccttgagtgttgcagtcatgctggttttgggcggtttttttattttgtgggtggagaaacgccaaagccgagcagagcctaaaattgccgatgttgatgcattgcgtccgattgatgccttgatgatcggcgttgcccaagtgtttgcactggttccgggtacgtcccgttcgggcagtacgattatgggcgggatgctttggggcatcgaacggaaaactgcgacagaattctcgtttttcttggctgtgccgatgatggttgccgcaacggcttatgatgtcctgaaacattaccgatttttcaccctgcatgatgtcggtttgattctgataggctttattgctgcctttgtttcaggcttggtagcggtaaaagcgttgctgaggtttgtttccaagaaaaattatattccttttgcctattaccgcattgtttttggtattgccatcattatattgtggctgtcaggctggataagttgggaatgaaaccataaacccgacctgaagacattattcgggtcgggtttgtctggcgggctgatatagtgaattaacaaaaatcaggacaaggcgacgaagccgcagatagtacggcaaggcgagccaacgctgtaccggtttaaatttaattcactataaaatcaggacaggcggggcgataggtttaaagtcgattgcctgttttgaaggcagtggtttattctttatttgctggcaatcaggcaataaaaaagcacatacctttttacggtctgtgcttttttatctggtggaggtaagcgggatcgaaccgctgacctcttgcatgccatgcaagcgctctaccaactgagctatacccccgaaaatttggtggcgaatcagggactcgaaccccggacacaaggattatgattcctctgctctaaccgactgagctaattcgccgtttcgtgaagacgctattatatgtttttctgtttttttgacaagccgtattttttaattttgaattagttgactgtttttaaatgttaaaaagtttatgccgtctgaagcggattcaggcggcatgagggttagagtttgtggcagatgtcgccgaagcggaatcctgcccagtcgatgccgatattttttccgaatgcgatgactttaaacagttcgcccatttcatgctggtcaatcagtttctgaacggcagcagcttcacagatgtaggctgccgaatccgttttccccgtctgtgccaatagctcggtaatgcccaagttcaataagaaatgggattggggaaggtaacctatcaaatctaatccggcatccgtccctgcttgtgcaatgtcggtaaagttgacatgtgcggtcaggtcggccaatccgatgaagtcaaaaggattgtggataatgtgatgtcggtagtgtccgatcagagtaccttgattgcgttgagggtggtaatactgcgctgcatcaaaaccgtagtcgatgaatatcatgcagccgtgttcgagtcttgaggcaagggtgcggataaaggcatattgttgcggatgtagttcgctggtatagggataatctgtttgaggaaaatagagggaagccaaggcagatagctgcaagtcgtgcagcggtcgtgccgaataggtaaaacggtcattatctaggcaaacgccgacatgctcgaatgagccgccttcatttttacggacgatttcgacaggcatggcatcgagtacttcgttgccgatgatgatgccgtcaaacgcttcgggaagtgcggtcaagtggacaactttttgagatgcttccggtgcgcgtgcttgaatcaggtttttctgacgtgctgccagctccggcgatatttcaataatatagtaacggctgatgccgtccgaaatgctgcccaacaaatcggcggcaagctgtccggttcccgcgccgaattcatagatattgcccgccgtttgggatagaagttcttgaagttggcgtgccagtgtctgtgcaaacagagaggtgagggtcggtgcggtaataaaatccccggtattgccgattttatggctgccgccggtgtagtagccgtattgcggagcgtataaaaccaattccataaaacgtgaaaatggaatccagttgccgtgtttgccgattttttcggcaatgagggtttgcagtttgagcgagaattgccgtgcttcgggagaggggaggggcatgataagtgttagcttgtgtaaatttattggatttcccgacatattacacgttggtacgggtgctgtcatggctttatcttaatactatatattgtgtttatattattaaattaatcatatatagttgtttattggttcgattattctgtaccgcacccgccgtgccgttgtcgtcattttttatcttattgtttttaaaaggaataaaaatttcagatatgttaatgagttttcatgccctgatttgaccgagtgtttaaaatttcttatagtgtcgattggtggggaattgtggggcaaagtgtctcttttacccttgtgattttgatttcggcttgggacatgtcatgttcggcggcgcacacgaattaagcatcgacagtaaggggcggttggctgttcctgccaaattccgtgacattctgtcgcgcctctatacgcctgccgtagtggtaacgctcgagtcgaaacacaagctgttgatgtaccctgttgcggagtgggaaaaggttgcggcgcaacttttaaacttaaaagtggcggataaccctgttttgcggcggtttcaaaatcttttgctgcataacgcggaaattttggaatgggacagcgccggccgggtgctggtttctgccggactgaggaagagggtggatttcgaccgtgaagtcgttttggtcggtcgtgccaaccgtttggagctttggggtcgcgagcagtgggaggctgagatggttcaggctttggatgacgatcctgacgaacttgccttccagttgagtcagacggatttgcaattgtgagtggagcagaaagttaccggcatatcacggtcttgctgaatgaggcggtggatgcgcttgccgtgcgcgaagacggtgtctatgtggacggtacgttcggcaggggagggcattcccggctgattttgtcgcgtttgggcgatgcggggcggttgattgttttcgacaaagacccgcaggcgattgctgtggcagaagagctggcgcgttcggacaaacgggtcggtgtcgtgcatggcggttttgcttcgtttcagacggcattggacggtttgggtatcggcaaggtggacggtgcgctgtttgatttggggatttcgtccccgcaaatcgatgacggcag

附录Bccgcggtttcagcttccgtttcgatgcccctttggatatgcgtatggatacgacgcgcggtatgtctgccgcagagtggatagcggttgcgtcggaacaggatttgcacgaggtaatcaagaattatggtgaagagcggtttagccgccggattgcgcgcgccattgttgcgcaacgggcggaaagtccaatcgatacaacccgcaagctggcgcagatcgtggcacaaaacgtccgtactcgcgagcgggggcaggatcctgcgacgcgcaccttccaggcggtccgcatctttattaaccgcgagcttgaagaagtaggggcagtattgccgcaggtcatgtgtcgtctgaaagagggcggacgtttggcggtcattgctttccattcgttggaagatcgcattgtgaagcagtttgtcaaaaaatattcgcaacacgcgcccctgccgcgctgggcggcggtcagggaagcggatttgcccgagctgcccctgaaaatcgtgggcagggcattaaagccgggtgaggcggaaattgccgccaatccgagggcgagaagtgcggttttgcgtgtggcggagcggactgccggtccgataccggaacaatcacagagaaaaacgtctgaatggcaatgaacaaattgaatttccttctgctgcttgcggtgtgcgtttccgctttttccgttgtgatgcagcaaaaccagtacaggctcaatttcacagctttggataaggcgaaaaaacaggaaatcgccttggagcaggattatgcgcaaatgaggctgcaacaggcgcgtttggcgaaccacgaagcgatcagggcggcggcagaaaaacaaaacctccatccgccggtttcgggcaatacctttatggtggagcatcaaagatagaagcagcctgtgtgccggaatcggattcctgcgtcaggataataataacgagaagtaaaaatgttgattaagagcgaatataagcctcggatgctgcccaaagaagagcaggtcaaaaagccgatgaccagtaacggacggatcagcttcgtcctgatggcaatagcggtcttgtttgccggtctgattgctcgcggactgtatctgcagacggtaacgtataactttttgaaagaacagggcgacaaccggattgtgcggactcaaacattgccggctacacgcggtacggtttcggaccggaacggtgcggttttggcgttgagtgcgccgacggagtccctgtttgccgtgcctaaagagatgaaggaaatgccgtctgccgcacaattggaacgcctgtccgagcttgtcgatgtgccggttgatgttttgaggaacaagctcgaacagaaaggcaagtcgtttatctggattaagcggcagctcgatcccaaggttgccgaagaggtcaaagccttgggtttggaaaactttgtatttgaaaaagaattaaaacgccattacccgatgggcaacctgtttgcacacgtcatcggatttaccgatattgacggcaaaggtcaggaaggtttggaactttcgcttgaagacagcctgcatggcgaagacggcgcggaagtcgttttgcgggaccggcagggcaatattgtggacagcttggactccccgcgcaataaagccccgaaaaacggcaaagacatcatcctttccctcgatcagaggattcagaccttggcctatgaagagttgaacaaggcggtcgaataccatcaggcaaaagccggaacggtggtggttttggatgcccgcacgggggaaatcctcgccttggccaatacgcccgcctacgatcccaacaggcccggccgggcagacagcgaacagcggcgcaaccgtgccgtaaccgatatgatcgaacccggttcggcaatcaaaccgtttgtgattgcgaaggcattggatgcgggcaaaaccgatttgaacgaacggctgaatacgcagccttataaaatcggaccgtctcccgtgcgcgatacccatgtttacccctctttggatgtgcgcggcatcatgcagaaatcgtccaacgtcggcacaagcaaactgtctgcgcgtttcggtgccgaagaaatgtatgacttctatcatgagttgggcatcggtgtgcgtatgcactcgggctttccgggcgaaactgcaggtttgttgagaaattggcgcaggtggcggcctatcgaacaggcgacgatgtctttcggttacggcctgcaattgagcctgctgcaattggcgcgcgcctataccgcactgacgcacgacggcgttttactgccggtcagctttgaaaaacaggcggttgcgccgcaaggcaaacgcatattcaaagaatcgaccgcgcgcgaggtacgcaatctgatggtttccgtaaccgagccgggcggcaccggtacggcgggtgcggtggacggtttcgatgtcggcgcgaaaaccggcacggcgcgcaagttcgtcaacgggcgttatgccgacaacaaacacatcgctacctttatcggttttgcccccgccaaaaatccccgtgtgattgtggcggtaaccattgacgaaccgactgcccacggttattacggcggcgtagtggcagggccgcccttcaaaaaaattatgggcggcagcctgaacatcttgggcatttccccgaccaagccactgaccgccgcagccgtcaaaacaccgtcttaatccgagtatcaacgagattgttttatgttcagcaagttaacccctttggctgaaaccggcatcccgactctgtcgtgtgcaaacgcggcagggcgtttgttgcattcagacagccgccaaatcaaacaaggcgatattttcgttgcctgtccgggcgaatatgccgacggacgcagttatatccccgccgccgttgccaacggcgcggcttttgttttttgggacgacgacggcaaatttgcgtggaatcccgaatggaaagtccccaatcaaggcatcaaagatttgaaacaccgtgccggcatattggcggcgcaagtttacggcaacgtttcagacggcctcaaagtttggggcgtagccggaaccaacggcaaaacctccatcacacaatggctggcgcaagctgccgatttgttgggcgaaaaaaccgccattgtcggcacggtcggcaacggcttttggggtgcattggaagaaaccacgcataccacacccgcccccgtcgatgtccaaaccctgctctaccgtttccgtcaacaaggcgcaacagtcgccgcgatggaagtctccagccacgggcttgaccagtcgcgcgtcaacggcgtgtcattccgcagcgcaatctttaccaacctcacccgcgaccacctcgactaccacggcacgatggaagcctacggtgccatcaagtcgcgcctgttttactggcacggcttgaaacacgcagtcatcaacgtggatgacgaatacggcgcggaactcgtaggtcgtctgaaaaaagactgtcccgatttggccgtttacagctatggtttcagcgaacacgccgacatccgcattaccgactttaccgcctcttcagacggcatagcagccgtattccaaaccccgtggggcgaagggaaatgccgcacgcgcctgctcggacggttcaacgcgcaaaacctcgccgcctgcatcgccttgctgtgcgccaacggctatccgcttgataaggtattggatgtgctggcaaaaatccgtcccgcttcagggcgcatggactgcatcatgaacagcggcaagcccttggtcgttgtcgattatgcccacacgcccgacgcattggaaaaagcactcgccaccttgcaggaaatcaaaccgcagggtgcggctttatggtgcgtattcggttgcggcggcaaccgcgatcgcggcaaacgcccgctgatgggcgcggcagccgtacagggcgcggataaagtcgtcgtcaccagcgacaacccgcgtttggaaaatccgcacgacatcatcaacgacatcctgcctgccgttcccgcgcccgaatgcgtcgaagccgaccgtgccgccgccgtccgttatgcggttgaacaagccgccgcaaacgacatcatcctgattgccggcaaagggcatgaaaactatcaggatgtacaaggcgtgaagcaccgtttttccgatcttgaaatcgtcggacaggctttgttaactcgtaaataatgggatattcggacggcatcgtatgaaacaatccgcccgaataaaaaatatgaatcagacattaaaaaatacattgggcatttgcgcgcttttagccttttgttttggcgcggccatcgcatcaggttatcacttggaatatgaatacggctaccgttattctgccgtgggtgctttggcttcggttgtatttttattattattggcacgcggtttcccgcgcgtttcttcagttgttttactgatttacgtcggcacaaccgccctatatttgccggtcggctggctgtatggtgcgccgtcttatcagatagtcggttcgatattggaaagcaatcctgccgaggcgcgtgaatttgtcggcaatcttcccgggtcgctttattttgtgcaggcattatttttcatttttggcttgacagtttggaaatattgtgtatcgggggggggtatttgctgacgtaaaaaactataaacgccgcagcaaaatatggctgactatattattgactttgattttgtcctgcgcggtgatggataaaatcgccagcgataaagatttgcgagaacctgatgccggcctgttgttgaatattttcgacctgtattacgatttggcttccgcgccggcacaatatgccgccaagcgcgcccacattttggaagcagcaaaaaaagcgtcaacatggcatatccgtcatgttgcgcccaagtataaaaattatgttgtggttatcggtgagagcgcgcgttcggattatatgaatgtttacggtttcccattgcccgatacgccttttttgagtcagaccaaagggctgttgataaacggttaccaatcgaccgcccacgcgacgaatctttcgctgccgcagactttggggctgccgggagaaccgaacaataacatcgtcagcttggcgaagcaggcgggttttcggacggcgtggctgtctaatcaaggaatgttggggcattttgccaacgaaatttccacctatgccctacgcagcgattatccgtggtttacccaaaggggtgattatggcaaaagcgcggggttgagcgaccgccttttgttgccggcgttcaaacgggttttgataggaaatgcaggcacgaagcctcggctgattgtgatgcacctgatgggttcgcacagtgatttttgcacacgtttggataaggatgcgcggcggtttcagtatcaaactgaaaaaatatcctgctatgtttccaccatcgcgcaaaccgataaatttttagaagatacagttaagatattgaatgaaaataaagaaagctggtctttggtttacttttccgaccacggtttgatgcatgtcggtaaaggcggcgagcgaacgttgacacatggtgcgtggaagcgtcaaagctacggcgtgccgctggttaaaatttcgtccgatgacacgcggcgcgaaatgattaaagtgaggcgcagcgcgtttaattttttacgcggattcggcagttggacgggtatcgaaaccgacgagttgcccgatgacggctatgatttttgggggaatgttcccgatgtgcagggcgaaggcaataaccttgcctttatcgacggactgcccgacgaccccgcgccgtggtatgcgggaaaaggcaaatcgactaaaaatacgtctaaaaaatgatacgtacagaaaaaatgccgaatgagaatgggaaaataatctgtgttttaccacagcaaaacaggcgataaaaaaatcagccgctaccgatgtgtccgccgcccgaatattaacgaaagtaaatatgaaaccactggacctaaatttcatctgccaagccctcaagcttccgatgccgtctgaaagcaaacccgtgtcgcgcatcgtaaccgacagccgcgacatccgcgcgggcgatgtgtttttcgcattggcgggcgagcggtttgacgcgcatgattttgttgaagacgtattggctgctggtgcggcggcggttgtggtttcgcgcgaagattgtgctgcaatggatggcgcgttgaaagtcgatgacacgcttgccgcattgcaaacgctggcaaaggcgtggcgtgaaaatgtgaatccgtttgtgttcggcattaccggttcgggcggcaagacgacggtgaaggaaatgctggctgcggtattgcgccgccgtttcggcgatgatgccgtgttggcgacggcaggcaacttcaacaaccatatcggattgccgctgactttgttgaagttaaacgaaaaacaccgctatgccgtgattgaaatgggcatgaaccatttcggcgaactggcggttttaacgcaaatcgccaaaccaaatgccgcattggtcaacaacgccatgcgcgcccatgtcggctgcggtttcgacggagtgggcgatattgccaaagcgaaaagcgagatttaccaaggtttatgttcagacggcatt

附录Bgcactgattcctcaagaagatgccaatatggctgtcttcaaaacggcaacgcttaatttgaatacgcgcactttcggcatcgatagcggcgatgttcacgcggaaaatattgtgctgaaaccgttgtcgtgcgaatttgatttggtgtgcggcgatgagcgcgccgccgtggtgctgcctgttcccggccgccacaatgtccacaacgccgccgctgccgccgcgctggctttggctgcgggtttgagtttgaacgatgtggcggaaggtttgaaaggcttcagcaatatcaaaggccgtctgaacgtcaaatccggaatcaagggcgcaaccctgattgacgatacttataatgcgaaccctgacagcatgaaagctgcgattgacgtgttggcgcgtatgcctgcgccgcgtattttcgtgatgggcgatatgggcgaactgggcgaactgggcgaggacgaagccgccgctatgcacgccgaagtcggcgcgtatgcccgcgaccaaggcatcgaagcggcttattttgtcggcgacaacagcgtcgaagcggcggaaaaatttggcgcggacggtttgtggttcgccgccaaagacccgttgattcaagtgttgcgccacgatttgcccgaacgcgccaccgtgttggtgaaaggttcgcgctttatgcagatggaagaagtggtcgaggcattggaggataagtgaaaatgaaaagccgacgtttttttaaagccttattgctgattgccgcgctggtcggcgcgttttatgccggaatgcggacgcaggcgtatctttatgaagatttatgtttagacttgggcggcggtaaaaatccggggagttacccaatttgcgtgattgagaaagtccctgcacgttaatctgcaaaagccgtccgaaaccttgccgggcggcaagccaacctcaaacgggcgcaggcccgatgtatagtggattaacaaaaatcaggacaaggcgacgaagccgcagacagtacaaatagtacggaaccgattcacttggtgcttcagcaccttagagaatcgttctctttgagctaaggcgaggcaacgccgtactggtttttgttaatccactataacgacaaaacaaaaaaaggaagccccatgtttttatggctcgcacatttcagcaactggttaaccggtctgaatatttttcaatacaccacattccgcgccgtcatggcggcgttgaccgccttagcgttttccctgatgttcggcccgtggacgatacgcaggctgaccgcgctcaaatgcgggcaggcagtgcgtaccgacggtccgcaaacccacctcgtcaaaaacggcacgccgacgatgggcggttcgctgattctgaccgccattaccgtgtccaccctgttgtggggcaactgggcaaacccgtatatctggattctcttgggcgtattgctcgccacgggcgcactcggtttttacgacgactggcgcaaagtcgtctataaagaccccaacggcgtgtccgccaaattcaaaatggtgtggcagtcaagcgttgccattatcgccagtttggcattgttttaccttgccgccaattccgccaacaatattttgattgtcccgttcttcaaacaaatcgccctgccgctgggcgtggtcggctttttggtgttgtcttacctgaccatcgtcggcacatccaatgccgtcaacctcaccgacggcttggacggccttgcgaccttccccgtcgtcctcgttgccgccggcctcgccatcttcgcctatgccagcggccactcacaatttgcccaatacctgcaattaccttacgttgccggcgcaaacgaagtggtgattttctgtaccgccatgtgcggcgcgtgcctcggtttcttgtggtttaacgcctatcccgcgcaagtctttatgggcgatgtcggtgcattggcattgggtgccgcgctcggtaccgtcgccgttatcgtccgccaagagtttgtcctcgtcattatgggcggattatttgtcgtagaagccgtatccgttatgcttcaggttggctggtataagaaaaccaaaaaacgcatcttcctgatggcgcccatccatcaccactacgaacaaaaaggctggaaagaaacccaagtcgtcgtccgcttttggattattaccatcgtcttggtgttgatcggtttgagtaccctcaaaatccgctgaacctatgccgtctgaacatctttcagacggcatttgaacgcgcaataaacctgcggcgacaatccgcccagccctatcgttaacggtggctgaaacccgccttatactaaaacagaagtaaaaccatgaaacagacagtcaaatggcttgccgccgccctgattgccttgggcttgaaccgagcggtgtgggcggatgacgtatcggattttcgggaaaacttgcaggcggcagcacagggaaatgcagcagcccaatacaatttgggcgcaatgtattacaaaggacgcggcgtgcgccgggatgatgctgaagcggtcagatggtatcggcaggcggcggaacaggggttagcccaagcccaatacaatttgggctggatgtatgccaacgggcgcggcgtgcgccaagatgataccgaagcggtcagatggtatcggcaggcggcagcgcagggggttgtccaagcccaatacaatttgggcgtgatatatgccgaaggacgtggagtgcgccaagacgatgtcgaagcggtcagatggtttcggcaggcggcagcgcagggggtagcccaagcccaaaacaatttgggcgtgatgtatgccgaaagacgcggcgtgcgccaagaccgcgcccttgcacaagaatggtttggcaaggcttgtcaaaacggagaccaagacggctgcgacaatgaccaacgcctgaaggcgggttattgaacagctcgcgatgccgtctgaaagcggcttgggcaggggcggacatctcctgctcaatatgatttgttttaggacaaaccaaaatgacttttcaaaacaaaaaaatcctcgtcgccggactcggcggtacgggtatttccatgattgcctacctgcgcaaaaacggcgcggaggttgctgcgtatgatgcggagctgaagccggaacgcgtgtcgcaaatcggtaagatgtttgacgggttggtgttttacacgggccgtctgaaagatgcgctggacaacggtttcgatattctggctctcagtcccggcatcagcgagcggcagccggatattgaggcgttcaagcaaaacggcggacgcgtgttgggcgacatcgaattgctggcggacattgtgaaccgccgggacgacaaggtaattgcgattaccggcagcaacggcaaaaccacggtaacgagcctggtcggctatctctgtatcaagtgcgggctggataccgttatcgcgggcaatatcggcacgccggttttggaggcggaatggcagcgcgaaggcaaaaaggcggacgtgtgggtgttggagctttccagcttccaactggaaaacaccgaaagcctgcgtccgactgcggcgacggtgctgaacatttccgaagaccatctcgaccgctacgacgacttgctcgactatgcgcataccaaagccaagattttccgtggcgacggcgtgcaggttttgaatgcggacgatgcgttctgccgcgcgatgaagcgtgccgggcgcgaggtaaaatggttttcgttggaacacgaagctgatttctggttggaacgcgagacaggccgcctgaaacaaggcaatgaagatttgattgtcacgcaagacattccgttgcaaggtctgcacaacgccgctaacgtcatggctgccgtggctttgtgtgaggccatcggtttgtcgcgcgaagcattgctcgaacacgtcaaaaccttccaaggcctgccgcaccgcgtggaaaaaatcggcgagaaaaacggcgtggtgtttatcgacgacagcaaaggcacgaatgtcggcgcgactgccgccgcgattgccggtttgcaaaatccgctcttcgtgattttgggcggcatgggtaaagggcaggacttcacgcccctgcgcgatgcactggtaggcaaggcaaaaggcgtgttcttgattggtgtcgatgcgccgcaaatccgccgcgatttggacggctgcggcttgaatatgaccgactgcgccactttgggagaagccgttcagacggcatatgcccaagccgaagcaggcgatattgtgttgctcagccccgcctgcgcgagctttgatatgttcaaaggctacgcgcaccgttcggaagtgtttatcgaagcgtttaaggctttgtgatgccgtctgaaatgcaaacgccgtcattgttgggcggcaagtaaagatttagaataccgatttgggatgtatcgtatgttcggacggcattgtctgccgtctgaaatttttgccctttgcggcaggtgcaaacagactggcaggtggtttttttgaagatttcggaagtattggtaaaagtgggcgacggtgtccacactctgctgctcgacaggccgattgtgcgcgacggcaggaaattcgacgcgccgcttttgtggatggtggtgctgatgacggcgttcagcctgctgatgatttattcggcttctgtgtatttggcatcaaaagaaggcggcgatcagtttttctatttgaccagacaggcggggttcgtcgttgccggcttgatagcgagcggtttgttatggtttctttgcaggatgaggacatggcggcggcttgtgccgtggatttttgccctatccggcctgttgctggtagtcgtattgattgccgggcgcgaaatcaatggcgcgacccgttggatacctttgggtccgttgaatttccagccgaccgagctgttcaagctggcggtcatcctttatttggcaagcctgttcacgcgccgtgaagaagtgttgcgcagcatggaaagtttgggttggcagtcgatttggcgggggacggccaatctgatcatgtccgccaccaatccgcaggcacgtcgtgaaacattagaaatgtacggccgtttccgggcgatcatcctgccgattatgctggtggcgttcggtttggtgctgataatggtacagccggatttcggttcgtttgtcgtcattaccgtcattgccgttggaatgctgtttttggcaggattgccgtggaaatattttttcgtcctggtaggcagcgtcttgggcgggatggtgctgatgattaccgccgctccctaccgtgtgcagcgggtagtggcatttttggacccgtggaaagacccgcagggtgccggctaccagcttacccactctctgatggcaatcgggcgcggagagtggttcggtatgggtttgggtgcgagtttgagcaaacgcggctttctgccggaagcgcataccgattttatttttgccatcatcgccgaagaattcggtttcttcggtatgtgcgtgctgatattctgttacggctggctggtggtgcgggcgttttccatcggcaagcagtcgcgcgatttgggtttgactttcaacgcctatatcgcttcgggtatcggcatttggatcggtatccaaagtttcttcaatatcggtgtgaacatcggtgctttgccgaccaaaggtctgacgctgccgttgatgtcctatggcggttcgtcagtctttttcatgctgatcagcatgatgctgctgttgcgtatagattatgaaaaccgccggaaaatgcgcggttatcgggtggagtaaatcatgggcggtaaaacctttatgctgatggcgggcggaacgggcggacatattttccccgcgctggcggtggcggattcattgcgcgcgcgcggccatcatgtgatttggctgggcagcaaggattcgatggaagagcgtatcgtgccgcaatacggcatacgcttggaaacgctggcgattaaaggcgtgcgcggcaacggcatcaaacgcaaactgatgctgccggttactttgtatcaaaccgtccgcgaagcgcagcggattatccgcaaacaccgtgtcgagtgcgtcatcggcttcggcggcttcgttaccttccccggcggtttggcggcgaagctattaggcgtgccgattgtgattcacgagcaaaacgccgtggcaggtttgtccaaccgccacctgtcgcgctgggcgaagcgggtgttgtacgcttttccgaaagcgttcagccacgaaggcggcttggtcggcaaccccgtccgcgccgatattagcaacctgcccgtgcctgccgaacgcttccaagggcgtgaaggccgtctgaaaattttggtggtcggcggcagtttgggcgcggacgttttgaacaaaaccgtaccgcaggcattggctttgctgcccgacaatgcgcgtccgcagatgtaccaccaatcgggacggggcaagctgggcagcttgcaggcggattacgacgcgctgggcgtgaaagccgaatgcgtggaatttattaccgacatggtgtccgcctaccgcgatgccgatttggtgatttgccgtgccggcgcgctgacgattgccgagttgacggcggcgggattgggtgcgttgttagtgccgtatc

附录Bctcacgcggttgacgatcaccaaaccgccaacgcgcgttttatggtgcaggcggaggcgggattgctgttgccgcaaacccagttgacggcggaaaaactcgccgagattctcggcggcttaaaccgcgaaaaatgcctcaaatgggcagaaaacgcccgtacgttggcactgccgcacagtgcggacgacgtggcggaagccgcgattgcgtgtgcggcgtaaactgccgaaccatgccgtctgaaaagccgttcagacggcatggatgttttttatttcaatccgctatatatttgtcagaaaactatggcgcgcaaacggtcagccctttaaaataacgcctttacgcatcgaaaatccaccggaacgcaacattatgatgaaaaatcgagttaccaacatccattttgtcggtatcggcggcgtcggcatgagcggcatcgccgaagtcttgcacaatttgggctttaaagtttccggttcggatcaggcgcgaaatgccgctaccgagcatttgggcagcctgggcattcaagtttatcccggccataccgccgaacacgttaacggtgcggatgtcgtcgttacctctaccgccgtcaaaaaagaaaatcccgaagttgtcgctgcgttggagcagcaaattcccgttattccgcgcgccctgatgttggcggagttgatgcgcttccgtgacggcatcgccattgccggcacgcacggcaaaaccacgaccaccagcctgaccgcctccatcctcggcgcggcaggacttgacccgactttcgttatcggcggcaaactcaacgccgcaggcactaacgcccgcttgggcaaaggcgaatacatcgttgccgaagccgacgagtcggatgcatcctttctgcacctgacaccgattatgtccgtcgttaccaatatcgacgaagaccatatggatacctacgggcacagcgtcgaaaaactgcatcaggcgtttatcgatttcatccaccgtatgcccttctacggcaaagcctttttgtgtattgacagcgaacacgtccgcgcgattttgcccaaagtgagcaaaccttatgctacttacggtttggacgataccgccgacatctacgccaccgacatcgaaaacgtcggcgcgcaaatgaaattcaccgtccatgttcaaatgaaaggacatgagcaggggtcgtttgaagtcgtgctgaatatgcccggcagacacaacgtgctgaacgcattggcagccatcggcgtggcgctggaagtcggcgcatcggttgaagcgatccaaaaaggcttgctcggctttgaaggcgtcggccgccgcttccaaaaatacggcgacatcaagttgccaaacggcgggaccgcgctcttggtggacgactacggacaccaccccgtcgaaatggcggcgacccttgccgccgcacgcggcgcgtatctggaaaaacgtttggtactcgccttccagccgcaccgctatacccgcacgcgcgatttgtttgaagactttaccaaagtcctcaataccgttgacgcgctggtgctgaccgaagtttatgccgccggtgaagagccgattgccgccgccgattcccgcgctcttgcccgcgccatccgcgtgttgggcaaactcgagccgatttactgcgaaaacgttgccgatctgcccgaaatgctgttgaacgttttgcaggacggcgacatcgtgttgaatatgggcgcgggaagcatcaaccgcgtccccgccgcgctgctggcattgtcgaaacagatttgaggcacacccgcctgacagacggaacatcatataaagatcgtctgaaaccgcaaatcaggtttcagacgacctctggcaacaagcataaagcaatcaggaaagaacaaaaacaatgcagaattttggcaaagtggccgtattgatgggcggtttttccagcgaacgagaaatctcgctggacagcggcaccgccattttgaatgctttaaaaagcaaaggcatagacgcatacgccttcgatcctaaagaaaccccattgtctgaattgaaggcacaaggttttcagacggcattcaacatccttcacggtacttacggcgaagacggggcggttcagggtgcattggaactgttgggcattccctataccggcagcggtgtcgccgcatccgccatcggcatggacaaataccgctgcaaactgatttggcaggcattgggattgcccgttcccgagttcgccgtcctgcacgacgacactgatttcgatgccgtcgaagaaaaattgggcctgccgatgtttgtgaaaccggcggccgaaggcagcagcgtaggcgtggtaaaagtcaaaggaaaaggccgtctgaaaagcgtttacgaagaattgaaacaccttcagggcgaaatcattgccgaacgttttatcggcggcggcgaatattcctgccccgtcctgaacggcaaagggctgcccggcatacacatcattcccgcaaccgagttttacgactacgaagccaagtacaaccgcgacgacaccatttatcaatgtccttcggaagatttgaccgaagccgaagaaagcctgatgcgcgaactggcggttcgcggcgcgcaggcaatcggtgcggaaggctgcgtgcgcgtcgatttcctcaaagataccgacggcaaactctatctgttggaaatcaacaccctgcccggtatgacgagccatagtttagtaccgaaatccgctgccgttacgggcgtgggttttgccgatttatgtattgaaattttgaagaccgcacatgtgggataatgccgaagcgatggaacggctgacgcgctggctgcttgtcatgatggcgatgctgcttgctgcgtccgggctggtttggttttacaattcgaatcatctgcccgtcaagcaggtgtcgctgaagggcaacctggtttattccgataagaagacattgggcagtttggcgaaagaatacatccatgggaatattttgaggacggacatcaatggcgcacaggaggcctaccgccggtatccgtggattgcgtcggtcatggtgcgccgccgttttcccgacacggttgaggtcgtcctgaccgagcgcaagccggtcgcgcgttggggcgaccatgccttggtggacggcgaaggcaatgtttttgaagcccgcttggacagacccggaatgccggtattcagaggcgcggaaggaacgtctgccgaaatgctccgccgttatgacgaattttcgactgttttggcaaaacagggtttgggcatcaaagagatgacctatacggcacgttcggcgtggattgtcgttttggacaacggcatcaccgtcaggctcggacgggaaaacgagatgaaacgcctccggctttttaccgaagcgtggcagcatctgttgcgtaaaaataaaaatcggttatcctatgtggatatgaggtataaggacggattttcagtccgctatgcttccgacggtttacccgaaaaagaatccgaagaatagtgggaacaggtatcggacagattacggccgtgccgtctgaaacggtgcgacgcaaatttcaatcagttttaagagcagacgaacaatggaacagcagcaaagatacatcagcgtactggatatcggtacgtctaaagtcctcgcactgatcggggaagttcaagatgacgacaaaatcaacatcgtcggtttggggcaggctccttcacggggcttgcgcgcgggcatggtaaccaatatcgatgccaccgtccaagccatcaggcaggcggtcaatgatgccgagctgatggcggataccaaaattactcacgttaccacaggtatcgcaggcaaccacatccgcagtctcaattcgcaaggtgtggttaaaattaaagatggggaagtcacgcaggcagacatcgatcgcgccattgaaacggcaaaggcaatcaatatcccgcccgatcaaaaaattctcgatgccgtggttcaagactacattattgacacccaacttggcgtgagggagcccatcggtatgagcggtgtgcgtctggatacgcgggtgcacatcattaccggtgcaagtacggcagtgcagaatgtccaaaaatgtatcgagcggtgcggtttgaaaagcgatcagatcatgcttcagccgttggcaagcgggcaggcggtgctgactgaagatgaaaaagacctcggcgtatgcgtcatcgacattggtggcggaacgaccgatattgccgtttatatgaacggtgccatccgccatacgtccgtcattccggccggtggtaatctgattaccaaagatttgtccaaatcgttgagaacacctctcgatgccgccgagtacattaaaatccattatggcgtggcatcatgcgatacggaaggcttgggtgagatgattgaagttccgggcgtgggtgaccggacatcgcgtcaggtttccagtaaggttctggcagcaatcatcagtgcacggattcaggagatttttggcgtagtgctgggcgagctgcaaaaatcgggtttccccaaagaagtgctgaatgcgggtatcgttctgaccggcggtgtgtccatgatgaccgggattgtggaatttgccgaaaaaatcttcgatttgcctgtacgcaccggtgcaccccaagaaatgggcggtttgtccgaccgcgtccgcacaccgcgtttttctaccgctatcgggctgcttcatgcagcatgcaagctggaaggaaacttgccgcagccggaaaacggtgcagtgcaagagagggaagggggcggcggtttgttggcaagattgaaacggtggattgaaaacagcttctgaacaggtggattgccgtttgacaggtgagaagtattttgccagcagcaagatacttcttatataatgaataataatttatttaaaccgtcctctgaatggggcgagcaggagtttttgaatggaatttgtttacgacgtggcagaatcggcagtcagccctgcggtgattaaagtaatcggcttgggcggcggcggttgcaatgcaatcaataacatggttgccaacaatgtgcgcggtgtggagtttatcagtgccaatacggatgcgcagtctctggcaaaaaaccatgcggcgaagagaatccagttgggtacgaatctgacacgcggtttgggcgcgggcgcgaatcccgatatcggccgtgcggcagcccaggaagaccgggaagccattgaagaagccattcgcggtgcgaatatgctgtttatcacgaccggtatgggcggcggtaccggtaccggttccgcgccggttgttgctgagattgccaagtctttgggcattctgaccgttgccgtggttacccgaccgttcgcatatgaaggtaagcgcgtccatgtcgcacaggcagggttggaacagttgaaagaacacgtcgattcgctgattatcatcccgaacgacaaactgatgactgcattgggtgaagacgtaacgatgcgcgaagccttccgtgccgccgacaatgtattgcgcgatgcggtcgcaggcatttccgaagtggtaacttgcccgagcgaaatcatcaacctcgactttgccgacgtgaaaaccgtgatgagcaaccgcggtatcgctatgatgggttcgggttatgcccaaggtatcgaccgtgcgcgtatggcgaccgaccaggccatttccagtccgctgctggacgatgtaaccttggacggagcgcgcggtgtgctggtcaatattacgactgctccgggttgcttgaaaatgtccgagttgtccgaagtcatgaaaatcgtcaaccaaagcgcgcatcccgatttggaatgcaaattcggtgcggctgaagacgagaccatgagcgaagatgccatccggattaccattatcgctaccggtctgaaagaaaaaggcgcggtcgattttgttccggcaagggaggtagaagcggttgctccgtccaaacaggagcaaagccacaatgtcgaaggtatgatccgcaccaatcgcggtatccgcacgatgaaccttaccgctgcggatttcgacaatcagtccgtacttgacgactttgaaatccctgcgattttgcgtcgtcaacacaattcagacaaataatgtgctgtttgcccgtaaacctgctgcctcccgaatcggtttgtccggtttgggaggtatgtttttcaagatgttgcaatttcgtacggtttgcggtcggcggattcagatttttccacttgatacagactttcagatatggacacttcaaaacaaacactgttggacgggatttttaagctgaaggcaaacggtacgacggtgcgtaccgagttgatggcgggtttgacaacttttttgacgatgtgctacatcgttatcgtcaaccctctgattttgggcgagaccggcatggatatgggggcggtattcgtcgctacctgtatcgcgtctgccatcggctgttttgttatgggttttgtcggcaactatccgattgcactcgcaccggggatggggctgaatgcctatttcacctttgccgtcgttaagggtatgggcgtgccttggcaggttgcgttgggtgcggtgttcatctccggtctgatttttatcctgtt

附录Bcagcttttttaaagtcagggaaatgctggtcaacgcactgcctatgggtttgaaaatgtcgattgctgccggtatcggtttgtttttggcactgatttccctgaaaggcgcaggcattatcgttgccaatccggcaaccttggtcggtttgggcgatattcatcagccgtccgcgttgttggcattgttcggttttgctatggtggtcgtattgggacatttccgcgttcaaggcgcaatcatcatcaccatcttgaccattaccgtcattgccagcctgatgggtttgaatgaatttcacggcatcatcggcgaagtaccgagcattgcgccgacttttatgcagatggattttgaaggcctgtttaccgtcagcatggtcagtgtgattttcgtcttcttcttggtcgatctatttgacagtaccggaacgctggtcggcatatcccaccgtgccgggctgctggtggacggtaagctgccccgcctgaaacgcgcactgcttgcagactctaccgccattgtggcaggtgcggctttgggtacttcttccaccacgccttatgtggaaagcgcggcgggcgtatcggcaggcggacggaccggcctgacggcggttaccgtcggcgtattgatgctcgcctgcctgatgttttcacctttggcgaaaagtgttcccgcttttgccaccgcgcccgccctgctttatgtcggcacgcagatgctccgcagtgcgagggatattgattgggacgatatgacggaagccgcacctgcgttcctgaccattgttttcatgccgtttacttattcgattgcagacggcatcgctttcggcttcatcagttatgccgtggttaaacttttatgccgccgcaccaaagacgttccgcctatggtatggattgttgccgtattgtgggcactgaaattctggtatttgggctgattgattcgatattaaaaatgccgtctgaaaggttttcagacggcattttgtttgccgatatatttaatttttattaaattatataaaaatcaaatacataataaaatacatcggattgcttaaaaataatacattgtttttatgtataaaatattttataagttttcaggattttgattatcaaaaatttttcttgatttcctgacaattttattgaaacaaataattcaaaattaatctagtttaatcatggaattaaaataaaatattaaaattatgtaatgagtctccttaaaaatgtttgacattttcagtcttgtgttttagattatcgaaaaataaaactacataacactacaaaggaacattactatgaaaccaattcagatgttttccccttttctgaataatccccttgttttcttcttgtctgcggttttgccgcataattccgaacggtctgctgtttttctttgattcgttttaaatatcaataagataatttttcccatatatttttaatgattggattgggatgcccgacgcgtcggatggctgtgttttgccgtccgaatgtgatggaagcctgtccatactgaaaaaaagtctataaaggagaaatatgatgagtcaacactctgccggagcacgtttccgccaagccgtgaaagaatcgaatccgcttgccgtcgccggttgcgtcaatgcttattttgcacgattggccacccaaagcggtttcaaagccatctatctgtccggcggcggcgtggcagcctgttcttgcggtatccctgatttgggcattaccacaatggaagatgtgctgatcgacgcacgacgcattacggacaacgtggatacgcctctgctggtggacatcgatgtgggttggggcggtgcattcaatattgcccgtaccattcgcaactttgaacgcgccggtgttgcagcggttcacatcgaagatcaggtagcgcaaaaacgctgcggccaccgtccgaacaaagccattgtatctaaagatgaaatggtcgaccgtatcaaagctgccgtagatgcgcgcgttgatgagaacttcgtgattatggcgcgtaccgatgcgctggcggtagaaggtttggatgccgctatcgaacgcgcccaagcttgtgtcgaagccggtgcggacatgattttccctgaagccatgaccgatttgaacatgtaccgccaatttgcagatgcggtgaaagtgcccgtgttggcgaacattaccgagtttggttccactccgctttatacccaaagcgagctggctgaaaacggcgtgtcgctggtgctgtatccgctgtcatcgttccgtgcagcaagcaaagccgctctgaatgtttacgaagcgattatgcgcgatggcactcaggcggcggtggtggacagtatgcaaacccgtgccgagctgtacgagcatctgaactatcatgccttcgagcaaaaactggataaattgtttcaaaaatgatttaccgctttcagactgcctttcaacaaatccgcatcggtcgtctgaaaacccgaaacccataaaaacacaaaggagaaataccatgactgaaactactcaaaccccgaccctcaaacctaaaaaatccgttgcgctttctggcgttgcggccggtaataccgctttgtgtaccgttggccgtaccggcaacgatttgagctatcgcggttacgacattctggatttggcacaaaaatgcgagtttgaagaagtcgcccacctgctgattcacggccatctgcccaacaaattcgagctggccgcttataaaaccaagctcaaatccatgcgcggcctgcctatccgtgtgattaaagttttggaaagcctgcctgcacatacccatccgatggacgtaatgcgtaccggcgtatccatgctgggctgcgttcatcctgaacgtgaaagccatccggaaagtgaagcgcgcgacatcgccgacaaactgatcgccagcctcggcagcatcctcttgtactggtatcaatattcgcacaacggcaaacgcattgaggttgaaagcgacgaagagaccatcggcggtcatttcctgcaactgttgcacggcaaacgcccaagcgaatcacacatcaaagccatgcacgtttcactgattctgtatgccgaacacgagttcaacgcttctacctttaccgcccgcgtgatcgccggtacaggctctgatatgtactccagcattaccggagcaatcggcgcgttgaaaggtccgaaacacggcggcgcgaacgaagtggcttacgatattcaaaaacgctaccgcaatgccgacgaagctgaagccgacatccgcgaacgcatcggccgcaaagaaatcgtgatcggtttcggtcatccggtgtacaccatttccgaccctcgcaacgttgtcattaaagaagtggcacgcggtttgagcaaagaaaccggcgatatgcgcctctttgacattgccgaacgtttggaaagcgtgatgtgggaagagaaaaaaatgttcccgaatctggactggttctctgccgtttcctaccaaaaattgggcgtaccgaccgctatgttcacaccgctgttcgtaatttcccgtacaaccggttggagcgcacacgttcttgagcaacgcaaagacggcaaaatcatccgtccgagcgcaaactacacaggccctgaagatttggcgtttgtggagattgaagaacgataattgaagaatgcaatagcagtttgttctttaatttcggtatgcaaagctaaggatttcagacgaccttgccttattggaaaggttgtctgaaataagtttaatctaataggagaagataatcctgtattggcgcaagtaacaggataagaaacatggaagatttatatataatactcgctttgggtttggttgcgatgattgccggatttatcgatgcgattgcgggcgggggtggtttgattacgctgcccgcactcttgttggcaggtattcctcccgtgtcggcaattgccaccaacaagctgcaagcagccgctgctacgttttcagctacggtttcttttgcacgcaaaggtttgattgattggaagaaaggtctcccgattgccgcagcatcgtttgtaggcggcgtggccggtgcattatcggtcagcttggtttccaaagatattctgctggcggtcgtgccggttttgttgatatttgtcgcactgtattttgtgttttcgcccaagctcgacggcagtaaggaaggcaaagccagaatgtctttttttctgttcgggctgacggtcgcaccgcttttgggtttttacgacggtgtgttcggaccgggtgtcggctcgttttttctgattgcctttattgttttgctcggctgcaagctgttgaacgcgatgtcttacaccaaattggcgaacgttgcctgcaatcttggttcgctatcggtattcctgctgcacggttcgattattttcccgattgcggcaacgatggcggtcggtgcgtttgtcggtgcgaatttaggtgcgagatttgccgtccgcttcggttcgaagctgattaagccgctgctgattgtcatcagcatttcgatggctgtgaaattgttgatagacgagagaaatccgctgtatcagatgattgtttcgatgttttaaaccctttcagacgaccccttcaaaacgtcggctgaaacctcaaaccacaagaaaaacagatccacaggagaaccgacatggctgccaaccaacgttaccgcaaaccgctgcccggtacggatttggaatactacgacgcgcgtgcggcgtgtgaggacatcaagcccggctcttacgacaagctgccttacacgagccgcattttggcggagaatttggtcaaccgcgcggacaaagtcgatttgccgacgctgcaaagctggctggggcagttgatagaagggaagcaggaaatcgactttccgtggtatccggcgcgggtggtgtgccacgatattctggggcagaccgcgttggtggatttggcaggcctgcgcgatgcgattgccgaaaaaggcggcgatcctgccaaagtgaatccggtggtgcaaacccagctcatcgtcgaccactctctggcggtggagtgcggcggttacgatcctgatgccttccgcaaaaaccgcgaaatcgaagaccgccgtaacgaagaccgtttccacttcatcaactggacaaaaaccgcgtttgaaaatgtggacgtgattccggcgggcaacggcatcatgcaccaaatcaatctagaaaaaatgtcgcccgtcgtccaagtcaaaaacggcgtggctttccccgatacctgcgtcggtactgactcacatacgccgcacgtcgattcattgggcgtgatttccgtgggcgtgggcggattggaagcggaaaccgtaatgctgggacgcgcgtccatgatgcgcctgcccgatattgtcggcgttgagctgaacggcaaacggcaggcgggcattacggcgacggatattgtgttggcactgaccgagtttctgcgcaaagaacgcgtggtcggggcgtttgtcgaattcttcggcgagggcgcgagaagcctgtctatcggcgaccgcgcgaccatttccaacatgacgccggagttcggcgcgactgccgcgatgttcgctattgatgagcaaaccattgattatttgaaactgaccggacgcgacgacgcgcaggtgaaattggtggaaacctacgccaaaaccgcaggcttgtgggcagatgccttgaaaaccgccgtttatcctcgcgttttgaaatttgatttgagcagcgtaacgcgcaatatggcaggcccaagtaacccgcatgcccgttttgcgaccgccgatttggcggcgaaagggctggcgaagccttacgaagagccttcggacggccaaatgcccgacggctcggtcatcatcgccgcgattaccagttgcaccaacacttccaacccgcgcaacgttgttgccgccgcgctcttggcacgcaatgccaaccgtctcggcttgaaacgcaaaccttgggtgaaatcttcgtttgccccgggttcaaaagtagccgaaatctatttgaaagaagcgggcctgttgcccgaaatggaaaaactcggcttcggtatcgtcgccttcgcctgcaccacctgcaacggcatgagtggcgcgctggatccgaaaatccagaaagaaatcatcgaccgcgatttgtacgccaccgccgtattatcaggcaaccgcaacttcgacggccgtatccacccgtatgcgaaacaggctttcctcgcttcgcctccgttggtcgttgcctacgcgctggcaggcagtatccgtttcgatattgaaaacgacgtactcggcgttgcagacggcaaggaaatccgcctgaaagacatttggcctgccgatgaagaaatcgatgccgtcgttgccgaatatgtgaaaccgcagcagttccgcgatgtgtatgtaccgatgttcgacaccggcacagcgcaaaaagcacccagtccgctgtacgattggcgtccgatgtccacctacatccgccgtccgccttactgggaaggcgcgctggcaggggaacgcacattaagaggtatgcgtccgctggcgattttgcccgacaacatcaccaccgaccacctctcgccgtccaa

附录Btgcgattttggccgtcagtgccgcaggcgagtatttggcgaaaatgggtttgcctgaagaagacttcaactcttacgcaacccaccgcggcgaccacttgaccgcccaacgcgctaccttcgccaatccgaaactgtttaacgaaatggtgaaaaacgaagacggcagcgtgcgccaaggctcgttcgcccgcgtcgaacccgaaggcgaaaccatgcgcatgtgggaagccatcgaaacctatatgaaccgcaaacagccgctcatcatcattgccggtgcggactatggtcaaggctcaagccgcgactgggctgcaaaaggcgtacgcctcgccggcgtagaagcgattgttgccgaaggcttcgagcgtatccaccgcaccaaccttatcggcatgggcgtgttgccgctgcagttcaaacccgacaccaaccgccataccctgcaactggacggtacggaaacctacgacgtggtcggcgaacgcacaccgcgctgcgacctgaccctcgtgattcaccgtaaaaacggcgaaaccgttgaagttcccgttacctgctgcctcgatactgcagaagaagtattggtatatgaagccggcggcgtgttgcaacggtttgcacaggattttttggaagggaacgcggcttagaggtcgtctgaaaagcaagacgtagcgtgggtcgggttcaacattttgctcattcacgtaattctcgatatggcaggcatctactgtaaatcgtcattcccgcgcaggcgggaatccagaaagtggaattgaggaaaccttatttatccgatgagtttctgtgcggacaaatttggattcccgcctgcgcgggaatgacggggtttaataatctgccgtatcacaacacagtagccgtagattgtggcgaaccccgacagtttgcggaatcaaacggctttgtcggagtggcagcctaatgtacttctggaaagtgggtgtagcgtgggctttgcccgcgaaataaaggctgaattgacatggtatagaggattaacaaaaatcgggacaaggcggcgaagccgcagacagtacagatagtacggaaccgattcacttggtgcttgagcaccttagagaatcgttctctttgagctaaggcgaggcaacgctgtactggtttttgttaatccactataaatttaatccactatactgtaaatcgtcattcccgcgcaggcgggaatccagaaagtggaattgaggaaacctttttatccgatgagtttctgtgcggataaatctggattcccgcctgcgcgggaatgacggggtttaataatctgccgtatcacaacacagtagccgtagattggggcgaaccccgacagtttgcggaatcaaacggctttggtcggagtggcagcctaatccactataaaaatcgtgggcagagcccacgctacataaggagaatctagaaatgccgcaaattaaaattcccgccgtttactaccgtggcggtacatcaaaaggcgtgtttttcaaacgttccgacctgcccgaggcggcgcgggaagcgggaagcgcacgcgacaaaatcctcttgcgcgtactcggcagcccggatccctacggcaagcagatagacggtttgggcaacgccagctcgtccaccagcaaggcggtgattttggacaagtccgaacgcgccgatcacgatgtcgattaccttttcgggcaagtttccatcgacaaaccttttgtcgattggagcggcaactgcggcaacctcaccgctgccgtgggcgcattctccatcgaacagggcttggtcgataaaggcaagattccttcagacggcatctgcacggtcaaaatctggcagaaaaacatcggcaaaaccattattgcccatgtaccgatgcaaaacggcgcagttttggaaacaggcgattttgagctcgacggcgtaacgttcccggcagccgaagtacaaatcgaatttcttgatccagccgacggcgaaggcagtatgttcccaaccggcaatttggtcgatgaaattgatgtgccgaatataggccgtttgaaagccacgctcatcaacgcgggcattccgaccgttttcttgaatgccgccgacttgggctacacaggcaaagagttgcaagacgacatcaacaacgatgccgcggctttggaaaaattcgagaaaatccgcgcttacggtgcgctgaaaatgggtctgatcagcgacgtatccgaagctgccgctcgcgcgcacacgccgaaagtcgccttcgtcgcgcccgccgccgattacaccgcctccagtggcaaaaccgtgaacgccgccgacatcgatttgctggtacgcgccctgagcatgggcaaactgcaccacgcgatgatgggtaccgcctctgttgccattgcgaccgccgccgccgtacccggtacgctggtcaaccttgccgcaggcggcggaacgcgtaaagaagtgcgcttcgggcatccttccggcacattgcgcgtcggtgcagccgccgaatgtcaggacggacaatggacggccaccaaagcggtcatgagccgtagcgcacgcgtgatgatggaaggttgggtcagggtgcctgaggattgtttttaaattgacgtagcatgggtttgcccgcgagccataaaaaggtcgtctgaaaaacaagtaaacatcaaatcactgaccattcctttcccttgccctgtggcggaaggcggcaaatcacaaggaagaacacggaaaccccgataaaagacagcttcccgtattaccgtcattcccgcgcaggcgggaatccagacctgtcaatatggaggattggcaggggaaaacaggtttcgtgagttctacattctggattcccgccacagcctgtcctcgcgtaggcggggacggaataacgatagaaaatgcggcatacgctttgcccaaagaggccgtctgaaacaccttgcgcctgatgtctgcctttttcagacgaccccacaccaaaaaaacaaccacaaactacaaggagaaacatcatgtccgaccaactcatcctcgttctgaactgcggcagttcatcgctcaaaggcgccgttatcgaccgaaaaagcggcagcgtcgtcctaagctgcctcggcgaacgcctgaccacgcccgaagccgtcattacgttcaacaaagacggcaacaaacgccaagttcccctgagcggccgaaattgccacgccggcgcggtgggtatgcttttgaacgaactggaaaaacacggtctgcacgaccgcatcaaagccatcggccaccgcatcgcccacggcggcgaaaaatacagcgagtctgttttgatcgaccaggccgtaatggacgaactcaatgcctgcattccgcttgcgccgctgcacaaccccgccaacatcagcggcatccttgccgcacaggaacatttccccggtctgcccaatgtcggcgtgatggatacttcgttccaccaaaccatgccggagcgtgcctacacttatgccgtgccgcgcgagttgcgtaaaaaatacgctttccgccgctacggtttccacggcaccagtatgcgttacgttgcccctgaagccgcacgcatcttgggcaaacctctggaagacatccgcatgattattgcccacttaggcaacggcgcatccattaccgccatcaaaaacggcaaatccgtcgataccagtatgggtttcacgccgatcgaaggtttggtaatgggtacacgttgcggcgacatcgatccgggcgtatacagctatctgacttcccacgccgggatggatgttgcccaagtggatgaaatgctgaacaaaaaatcaggtttgctcggtatttccgaactttccaacgactgccgcaccctcgaaatcgccgccgacgaaggccacgaaggcgcgcgcctcgccctcgaagtcatgacctaccgcctcgccaaatacatcgcttcgatggctgtgggctgcggcggcgttgacgcactcgtgttcaccggcggtatcggcgaaaactcgcgtaatatccgtgccaaaaccgtttcctatcttgatttcttgggtctgcacatcgacaccaaagccaatatggaaaaacgctacggcaattcgggcattatcagcccgaccgattcttctccggctgttttggttgtcccgaccaatgaagaactgatgattgcctgcgacactgccgaacttgccggcatcttgtagccaaaaaagggacgagtccgcaaaaatgccgtctgaaaccccaaacgcccgattaggctgatgaggattttagacggcattgttcatttttttgttatcttgcatttttgtgcggacggtggaatttcatcctgtaaacataaatatttgtcggaaaacagaaaccctccgccgccatttctacgaaagcaggaaaccagcaacgcaaagcgacagggatttgttggaaatgaccgaaaccgaacgaaccggattcccgcctgcgcgggaatgacgggattttctgtttttgtggaaatgacgggattttgaatttcgggcgtacaatacggaaaacatgacgataaggaaacaaaccatggcacagtttttcgctattcatcccgacaatccccaagaacgcctcatcaagcaggcggttgaaatcgtcaataaaggcggcgtggtcgtttatccgaccgattcctgttatgccttgggctgcaaactcggcgataaggcggcgatggaacgcatactctccatccgcaaaatcgatttgaaacaccacctgaccctgatgtgcgcagatttgagcgagttgggcacatacgccaaagtcgacaacgtacagtttcgtcagcttaaagccgccacacccgggccttatacttttattttacaggcgacgaaggatgtgccggcgcgcacgctgcacccgaaacgcaaaaccatcgggctgcgtattcccgataatgccattgcacaagccctgctgggggaattgggcgagccgcttttaagctgcaccctgatgctgcccgaagacggcgaaccattgaccgatccttatgaaatccgcgagcgtttggaacacgccgtcgatttggtgattgacggcggctggtgcggaaccgagccgaccaccgtcgtcgatatgaccgacggcacggaattggtgcgccaaggttgcggcgatacggcggtgttcggtttgtagggaaaccgatgccgtctgaagcatcggctgttcagacggcattgcgcgccttgccggcggcagtccgaaatgccggcgcgtatcgcgctcggtcggaatatccgtttgaaacggcattttgatgcattactgcaccgcaatcggaattctcggttcgtagagcaggtcgtaggtcggcttgttgagcaggtcttggagcgtgaaaccgtccagatacgtgaaaaacgacttcatcgcgccgccgagtatgcccgtcagccggcaggacggtgtaatcaggcattcgttgttctcgcccatgcactcgaccagctgcatcggttcgaggtggcggacaaccgagccgatgttgatgcggtcgggcggtgcggcaagccgcagaccgccgccttttccgcgcacactgtggaggaagccgcctttgaccagcgcggtaacgaccttcatcagatggcttttggaaatgccgtaggttacggcgatggtactgatgttgaccagcgcatcgtcgttgatggcagtgtagataaggacgcgcagcccgtagtccgtatgttgtgtcaaatacatgattttctcggtatggattgttattcttatcggtacggtttaaggttcacggacaataccttaatggttgaaaccctgtccgtcggggcggtagaatgcagcctgtctgcggcggtatgccgtctgaaacatccgcgctaccgtttgagaatttgttattgtaactcaaaatcatgaaaccgttgaaacgacatcccgcccttatcgggctttcgcgtgaccaccaccattcgctttccctgtgcgtgcgtctgttgcggacgccggaagaaaggcatcgggacgaactcgaaccgcatttttccgaattggaaacccattttcgcgaagaagaaaccaagtttgccccaatttggcagaatgtcgcccccgaattgaaacaacgtttcgagaaagaccacgcccgactgcggcagatgatggcaagccccgaatacggtaacgcggcgtggaataccgcttttgccacaaccctgcgcgaccacgcgcgctttgaagaacgcgagctgtttcccgccgccgaaccgtttttgccggcatgattccgttttgcggtaaatatattaatgataaacaaggaacacacatgaaatttaccaagcaccccgtctgggcaatggcgttccgcccattttattcgctggcggctctgtacggcgcattgtccgtattgctgtggggtttcggctacacgggaacgcacgagctgtccggtttctattggcacgcgcatgagatgatttggggttatgccggactggtcgtcatcgccttcctgctgaccgccgtcgccacttggacggggcagccgcccacgcggggcggcgttctggtcggcttgactatcttttggctggctgcgcggattgcc

附录Bgcctttatcccgggttggggtgcgtcggcaagcggcatactcggtacgctgtttttctggtacggcgcggtgtgcatggctttgcccgttatccgttcgcagaatcaacgcaactatgttgccgtgttcgcgctgttcgtcttgggcggcacgcatgcggcgttccacgtccagctgcacaacggcaacctaggcggactcttgagcggattgcagtcgggcttggtgatggtgtcgggttttatcggtctgattggtacgcggattatttcgttttttacgtccaaacgcttgaatgtgccgcagattcccagtccgaaatgggtggcgcaggcttcgctgtggctgcccatgctgactgccatgctgatggcgcacggtgtgttggcttggctgtctgccgtttttgcctttgcggcaggtgtgatttttaccgtgcaggtgtaccgctggtggtataaacccgtgttgaaagagccgatgctgtggattctgtttgccggctatctgtttaccggattggggctgattgcggtcggcgcgtcttatttcaaacccgctttcctcaatctgggtgtgcatctgatcggggtcggcggtatcggcgtgctgactttgggcatgatggcgcgtaccgcgcttggtcatacgggcaatccgatttatccgccgcccaaagccgttcccgttgcgttttggctgatgatggcggcaaccgccgtccgtatggttgccgtattttcttccggcactgcctacacgcacagcatccgcacctcttcggttttgtttgcactcgcgcttttggtgtatgcgtggaagtatattccttggctgattcgtccgcgttcggacggcaggcccggttgagacaaaccgccgcagatttcgggtctgggcggtttgcttttcagacggcagggcggtcagttgccgtccagccagcggtcgcgtgtggttttggcttcttcaaaatagcggtacagggcttcgcggtcgtcggtggtcaggatgtttgccaaaacgtccaactgtttgcccaagccttgaaccagttgcagcaggctgtctttgttggcaaggcagatgtccgcccacacggcgggatgaccggaggcgatgcgggtgaagtcccgaaagcccgtggcggcgaatttcagatattcctgtccgtcggggtggtcgagaatctggtggacataggcgaaggcggtcaggtggggcatatgggagacggcggcgaaaaccgcgtcgtggcgttgcgcgtccatcgtataaatttccgcaccgaccgcgtgccacaggttttctaccaaggcaatgccgtctgaatgttcgccgccgtgtggcgtgatgatgagttttctgtggcggaacagcccgaactgcgcggcttgcgcaccgcttctgtccgaaccggcaattgggtgggcggcgatgcagtggtgcaggcggtcgggcagacagcggcggaaggcttcgatgaccgaagatttggtgctgccgacatcggaaatccaagtgtgttccggcaaaacggggcgcagcgcggtcaaaatggcgggaacggtggcgacgggcgtggcaatcagtaccaagtccgcaccgccgatgctgtccgcgtcgatggcaacggaagcctggtcaatcacgccgcgttccaatgcacgttcgaggttgtcgcggtcggtgtcgataccggtaacggtgcggacgagtccctgccttttgaggtcgagaacgaacgaaccgccgatcagccctacaccgatgagggcaatatggttcaaaatgggcatttgtgtaaacggttttcgcaaagtaccgtcatggtagcctatcggcggaatatgccgcaaggtcggcaggaaaaaggagaagaaatggacaaaatcagagttgccgccgtgcagatggtgtcgggcgtgtcgccggaaaccaacgtcgccgccatgaaacgcctggtcgcacgggcggcggagcagggtgcggattgggtgctgctgcccgaatattgggtgctgatgggcgcaaacgataccgacaaactcgcgcttgccgagcctttgggcggcggacgctttcagacggcattgagcgaaacggcgaaagaatgcggcgtggtgctgttcggcgggactgtgccgctgcaaagctgcgaggcgggtaaagtgatgaatacgctgttggtgtacggacgggacggcgtaaggacggggctgtaccacaaaatgcacctcttcggtttttccggtttgggcgaacgctatgccgaagccgataccatccgcgcgggcggggatgtgccgcacttgtcggcagaaggcgtgccggtggcggcgggcatttgttacgatgtccgctttcccgaatttttccgacgccagttgccgtttgacgtattgatgctgcccgctgcgtttacgcacacgacgggcaaggcgcattgggagctgctgctgcgcgcgcgtgccgtcgaaaaccaatgttacgtcgtggcggcggcacagggcggtttgcacgaaaacggacggcgcacgttcggacacagcatgattgtcgatccgtggggcgacgtgttggacgtattgcccgagggcgaaggcgttgttacggcagacatcgatgccaaccgcctgaacagcgtccgcaaccgcctgcccgccttgaaataccgggttttggatgccgtctgaaggttcagacggcatcggtgccggggaatcagaagcggtagcgcatgcccaatgagacttcgtgggttttgaagcgggtgttttccaagcgtccccagttgtggtaacggtatccggtgtccaaggtcagcttgggcgtgatgtcgaaaccgacaccggcgatgacaccaagacccacgctgctgatgctgtggctttcgtgatagggaggtttgctgggatcagtttgtataatagggcctccctgtggagagccgttctttggtttagaggtaatagtcgtggtttttgtttccaccgaatgaacttgatgtttaacgtgtccgtaggcgacgcgcgcgccgatatagggtttgaatttatcgttgagtttgaaatcgtaaatggcggacaagccgagagaagaaacggcgtggaagctgccgtttccctgatgttttgtttgggtttctttgtagttgttgtttatctcttcagtaacttttttagtagaagaattactttctttccattttctgtaactggcataatctgccgctattctccagccgccgaaatcatagccgaccgacacccgggggtggatggaatgcgcacggatgtttctgaaataatcgcttaccgtgcttgtgttgtttgcaccggttgcttgcggataatcgtgggtaatgcgttcggcggcataagctaaatccgcctgcacataatacgggctgcggctgccgtcttcacttgccgcctgcgctgcggaagagaagagaagagaagagaagagaagagaagagaagagaagagaagagaaggttttttgggggctggattcattttcgactccgtattcggttttaactgattaaaaagaaagattttcactgatgttgcaggggtggattgtatcgggtttggggcgatgtttcaacacaatatagcggatgaacaaaaaagagaacgatgctctaaggtgcccaagcaccaagtgaatcggttccgtactatagtggattaacaaaaaccagtacagcgttgcctcgccttagctcaaagagaacgattctctaaggtgctgaagcaccgagtgaatcggttccgtactatttgtactgtctgcggcttcgtcgccttgtcctgatttttgttaatccgctataaagaccgtcgggcatctgcagccgtcattcccgcgcaggcgggaatctagaccttagaacaacagcaatattcaaagattatctgaaagtctgagattctagattcccacgaaagtgggaatccaggatgtaaaatctcaagaaaccgttttatccgataagttcctgcactgacagacctagattcccgcctgcgcgggaatgacgggattttaggtttctgattttggttttctgtccttgtgggaatgacgggatgtaggttcgtaggaatgacgtggtgcaggtttccgtgcggatggattcgtcattcccgcgcaggcgggaatctagaccttagaacaacagcaatattcaaagattggcggattcgcatttgaagtgcaactttccctaacagaaaaaggccagtatgcggtagcatacggcctttcctgcaagaaagattgccatgagctacacgcaactgacccaaggcgaacgataccacatccaatacctgtcccgccactgcaccgtcaccgaaatcgccaaacagctgaaccgccacaaaagcaccatcagccgcgaaatcagacggcaccgcacccaagggcagcaatacagcgccgaaaaagcccagcggcaaagccagactatcaaacagcgtaagcgacaaccctataagctcgattcgcagctgattcagcacatcgacccccttatccgccgcaaactcagtcccgaacaagtatgcgcctacctgcgcaaacaccaccagatcacgctccaccacagcaccatttaccgctaccttcgccaagacaaaagcaacggcagcacgttgtggcaacatctcagaatatgcagcaaaccctaccgcaaacgctacggcagcacatggaccagaggcaaagtacccaaccgtgtcggcatagaaaaccgacccgctatcgtcgaccagaaatcccgtatcggcgattgggaagccgacaccattgtcggcaaaggacagaaaagcgcattattgaccttggtcgaacgcgttacccgctacaccatcatctgcaaattggatagcctcaaagccgaagacactgcccgggcagctgttagggcattaaaggcacataaagacagggtgcacaccattaccatggataacggcaaagagttctaccaacacaccaaaataaccaaagcattgaaagcggagacttatttttgtcgtccttaccattcttgggagaaagggctgaatgagaacaccaacggactcatccggcaatacttccccaaacaaaccgatttccgtaacatcagtgatcgggagatacgcagggttcaagatgagttgaaccaccgaccaagaaaaacacttggctacgaaacgccaagtgttttattcttgaatctgttccaaccactaatacactagtgttgcacttgaaatccgaatccaagattatctgaaagtctgagattctagattcccactttcgtgggaatgacgggattttaggtttctgattttggttttctgtccttgtgggaatgacgggatgtaggttcgtaggaatgacgtggtgcaggtttccgtgcggatggattcgtcattcccgcgcaggcgggaatttggaatttcaatgcctcaagaatttatcggaaaaaaccaaaacccttccgccgtcattcccacgaaagtgggaatctagaaatgaaaagcagcaggcatttatcggaaatgaccgaaactgaacggactggattcccgcttttgcgggaatgacggcgacagggttgctgttatagtggatgaacaaaaaccagtacggcgttgcctcgccttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttctgtactatttgtactgtctgcggcttcgtcgccttgtcctgatttttgttcatccgctatacttttgtatgaccatctgactttatcactcactatgttttaccaaatccttgccctgattatctggagcagctcgtttattgccgccaaatatgtctatggcggcatcgatcccgcattgatggtcggcgtgcgcctgctaattgccgcgctgcctgcactgcccgcctgccgccgtcatgtcggcaagattccgcgtgaggaatggaagccgttgctgattgtgtcgttcgtcaactatgtgctgaccctgctgcttcagtttgtcgggttgaaatacacttccgccgccagcgcatcggtcattgtcggactcgagccgctgctgatggtgtttgtcggacactttttcttcaacgacaaagcgcgtgcctaccactggatatgcggcgcggcggcatttgccggtgtcgcgctgctgatggcgggcggtgcggaagagggcggcgaagtcggctggttcggctgcctgctggtgttgttggcgggcgcgggcttttgtgccgctatgcgtccgacgcaaaggctgattgcacgcatcggcgcaccggcattcacatctgtttccattgccgccgcatcgttgatgtgcctgccgttttcgcttgctttggcgcaaagttataccgtggactggagcgtcgggatggtattgtcgctgctgtatttgggtttggggtgcggctggtacgcctattggctgtggaacaaggggatgagccgtgttcctgccaatgtttcgggactgttgatttcgctcgaacccgtcgtcggcgtgctgctggcggttttgattttgggcgaacacctgtcgcccgtgtccgccttgggcgtgtttgtcgtcatcgccgccaccttggttgccggccggctgtc

附录Bgcatcaaaaataaagttgggaagcggtatttgatgattgccgaataggctgaaatctttccatctccattcctgcgaaagcgggtatccggaacgaaaagacggatatttatccgaaataacgaccatctttgcgtcgtcattcccgcgcaggcgggcatccggttttttgagtttcggttatttccgacaaattgctgcagcgttggatgtccggatttccgcctgcgcgggaatgacgggattttatagtggattaacaaaaatcaggacaaggcggcgagccgcagacagtacagatagtacggaaccgattcacttggtgcttcagcaccttagagaatcgttctctttgagctaaggcaaggcaacgctgtactggtttttgttaatccactatatcgttccggttcgtccggttttgccggggcttttgttgccgcctgtttgtgccggtgtgttaaaattttccgtttccgcgtattgtgttttccgccgccgggcggtttgtttgcgaatcggacgagaatttatgccttctgcccattatcctgaaatgagcgaaaaactgatggcggttttgatggcgatgctggttacgctgatgccgttttccatcgatgcctacctgcccgcgattcccgaaatggcgcaatcgctgaacgcggatgttcaccgcatcgaacagagtttgagtttgtttatgttcggcacggcgttcggacaggtggtcggcggttcggtgtccgacatcaaagggcgcaaacccgtcgccctgaccggtttgattgtatattgccttgccgttgccgccatcgtatttgtttcgagtgccgaacagctcctcaacctgcgcgtcgtgcaggcattcggtgcgggcatgactgtggtcatcgtcggcgcaatggtgcgcgattattattccggacgcaaagccgcccagatgtttgcccttatcggcatcattttgatggttgtgccgctggtcgcacccatggtcggcgcattgttgcagggcttgggtggctggcaggcgatttttgtttttctggcggcgtattcgctggtgctgctcggtttggtacagtatttcctgcccaagcccgccgtcggcggcaaaatcggacgggacgtgttcgggctggtggcggggcggttcaagcgcgtattgaaaacccgtgctgcgatgggttatctgttttttcaggcattcagcttcggttcgatgttcgcctttctgaccgaatcttccttcgtgtaccagcagctctaccgtgttacgcctcatcaatacgcttgggcgtttgcactcaacatcatcacgatgatgtttttcaaccgcgttaccgcgtggcggctcaaaaccggcgtgcatccgcaaagcatcctgctgtgggggattgtcgtccagtttgccgccaacctgtcccaactcgccgccgtgctgtttttcgggttgcccccgttttggctgctggtcgcgtgcgtgatgttttccgtcggtacgcagggcttggtcggtgcaaacacgcaggcgtgttttatgtcctatttcaaagaagagggcggcagcgcaaacgccgtattgggtgtattccaatctttaatcggcgcgggggtgggtatggcggcgaccttcttgcacgacggttcggcaaccgtgatggcggcaacgatgaccgcgtccacctcttgcggcattgcgcttctgtggctctgctcgcatcgtgcgtggaaagaaaacgggcaaagcgaatacctttaacggaaaatgccgtctgaaaccgtttcagacggcatttgatgttagaatgcacgataaattactgttcaggcgaaattatgtcccaaactatcgacgaactcctccttccccaccgcaacgccatcgacaccatcgatgccgaaatcctgcgcctgctcaacgaacgtgcgcaacacgcccacgccatcggcgagctgaaaggcacgggcgcagtgtaccgccccgaacgcgaagtcgccgtgttgcgccgcattcaggatttgaacaaaggcccgctgcccgacgaatcggtagcacgcctgtttcgggaagtgatgagcgagtgcctcgccgtcgaacgcccgctgaccatcgcctatctggggccgcagggcacgtttacccagcaggcggcaatcaaacatttcggacacgccgcgcacaccatggcgtgtccgaccatagacgactgcttcaagcaggttgaaacgcgtcaggcggattatctggtcgcccccgtggaaaattcgaccgaaggctcggtcggtcgcacgttagacctgcttgccgttaccgcgttgcaggcgtgcggcgaaatcgttttgcgcatccaccacaaccttttgcgtaaaaacaacggcagcaccgaaggcattgccaaagtcttttcccacgcgcaggcgttggcgcagtgcaacgactggttgggcagacacctgcccaacgccgaacggattgccgtgtccagcaatgccgaagccgcaaggctggttgccgaatcggacgacggtacggttgccgccatcgccggacgcacggcggcggaaatctacggactcgatatggttgccgagtgcatcgaagacgaaccgaacaacaccacgcgcttcttggtgatgggacatcacgaaaccggtgcaagcggcagcgacaagacttcgctggccgtttccgcgcccaaccgggcaggcgcggttgcctcgctgctgcaaccgctgaccgaatcgggtatttccatgaccaagtttgagagccgtccgagcaaatccgttttgtgggaatacctgttcttcatcgacatcgaaggacaccgccgggacgcgcagattcagacggcattggaacgcttgggcgaacgcgcttcgttcgtcaaagtcatcggttcgtacccgaccgccgttttgtagcggcggcagcgttcagacggcatttccccaacgattatgtccgaataccgagtcaaccatgaacccgtttttatgctggcatcttcgccctggcgcgaaagcagcctgtgggttgaagcattcagccgccgttacgggcgtgtggctttgctggcgcgcagcgcgcgcaaaaggcagagcgagctgcgcggcgtattggtgccgttcgtgcccgtcagcgtgtcgtggtacggcagtcaggaactcaaaaccctacaccgcgccgaatgggtcggcggttggcggcagcctcagggcagggcgttgttcggcggattgtatgtgaacgagttggtgttgaaactgaccgcccgcgaagacccggtgcccgagttatacgacgcgttggcggaagtgatggaggcggtgtgctgcaaagccgcttatatcgacgacttgcgccgtttcgagtggcggctgctgaacctgttgggcgttgcccccgatttgaaccgcgacggggacggcgggacgattgcggcaggcggcacataccttgtccgcccggaaacagccgtcttccccgtcggaaaaggatttgccgtaccgccgcacgccgccggcgttgtcgcccccgggcagagcctgatcgatttgcgcgaaggcagtttccgcactgccgaaagcctgcaacaggcattgaaaatcacacggctttttatccgccacctgttgcccgaggggctgaaatcgcggcaggtgttggaacagatacggcagtttgaccgcaaagaaaccgcccgggaaaccgtcccgacttcggacggcacggcttcaaatgccgtctgaaggcagaaataaaaggaaagattatgcttttaggtgtcaacatcgaccacatcgccaccgtccgcaatgcgcgcggtacgacttatcccagccccgtggaggcggcactggttgccgaaacgcacggtgcggatttgattaccatgcacctgcgcgaagaccgccgccacatcaaagacgcggacgtgtttgccgtcaaaaacgccatccgcacgcgcctgaaccttgaaatggcgttgacggaagaaatgttggaaaacgctttgaaagtgatgccggaagacgtgtgcatcgtgcctgaaaaacgtcaggaaatcacgaccgaaggcggtttggacgtattggcgcaacaggaaaaaatcgccgggttcaccaaaatcctgaccgacgcaggcatacgcgtgtctttgtttatcgatgccgacgacaggcaaatccaagccgcccgtgatgtcggcgcgcccgttgtcgagctgcacacaggcgcgtatgccgacgcgcgcagccacgccgaacaaatcaggcagttcgagcgcatccaaaacggcgcgcatttcgccggcgatttgggcttggtcgtcaacgccggacacggactgaccatacacaacgttacccccatcgcccaaatcctcgccatccgcgaactgaacatcgggcattcgctgattgcccaagccctcttcctcggactgcccgaagccgtgcgccaaatgaaggaggcgatgttcagggcaaggctgctgccgtaagggcaggcaaaccctttcagacagcatttcacgacagggatatgttatagtggattaaatttaaatcaggacaaggcggcgaagccgcagacagtacaaatagtacggcaaggcaagccaacgccgtactggtttaaatttaattcactatatgaatcaaaagtatattttatctgcaaacaataatagtttgatagaagaaattcacaatacagtacagagtattgggtattgtattgttcgaggtcttaatctaaaccatcttgatggcagccggagaaacaagaaattatttgactttctatctcaattaggaatgctgacaaaccacaaaggcgatggttttaaatctatattttgggatattaaatattgaggcgatgattatgtaatatagtggattaacaaaaatcaggacaaggcgacgaagctgcagacagtacagatagtacggaaccgattcacttggtgcttcagcaccttagagaatcgttctctttgagctaaggcgaggcaacgccgtactggtttttgttaatccactataaataatgatataactttctcggaagatgttggagaatgtccacttcatagtgattcatcttttagtgaaaacccggaaagttatttggttatgtatgtagtaaaatcagccaatgatggaggtaattccctatttttaagttcatcagatattgtcaatcagttatctaaaacagaaaccggtaaaaaacacttaaaaacattaacgggcaatttatatccatttaaaacaccagcatcatttgataaaaaacaaggtgtgagatggggtaatatcttatcggtcaatactcaaatgattagatttagaagtgattgtatctataaaggtattgaagaaaatagaaataaagtatcaaaggaaatggtacttgcacttgattatcttataaatgttataaaaaatgcgagtgatattcaagaattttctgcacaagatgatggtttgattattattgacaatgtcaatggcttgcatgccagaactgattatacggataaaaacaggcattatattagagcaagaattactgtataaaggacggttatgcaagaaataatgcaatctatcgtttttgttgctgccgcaatactgcacggaattacaggcatgggatttccgatgctcggtacaaccgcattggcttttatcatgccattgtctaaggttgttgccttggtggcattaccaagcctgttaatgagcttgttggttctatgcagcaataacaaaaagggtttttggcaagagattgtttattatttaaaaacctataaattgcttgctatcggcagcgtcgttggcagcattttgggggtgaagttgcttttgatacttccagtgtcttggctgcttttactgatggcaatcattacattgtattattctgtcaatggtattttaaatgtatgtgcaaaagcaaaaaatattcaagtagttgccaataataagaatatggttctttttgggtttttggcaggcatcatcggcggttcaaccaatgccatgtctcccatattgttaatatttttgcttagcgaaacagaaaataaaaatcgtatcgtaaaatcaagcaatctatgctatcttttggcgaaaattgttcaaatatatatgctaagagaccagtattggttattaaataagagtgaatacggtttaatatttttactgtccgtattgtctgttattggattgtatgttggaattcggttaaggactaagattagcccaaatttttttaaaatgttaatttttattgttttattggtattggctctgaaaatcgggcattcgggtttaatcaaactttaattcattattaaatgccttaactccttattaaataattggcacgatgttttagaatttcaaatgcaaaaggttacagtgaaaattgttaccgacaaaaccccaaaagtggatattcacgccattttaacgccccaagaaattgacggcattcatcatcacattcatcactacccgcaaccaagggcgaaggagcgcaaatatgatttacggcatcggcacagacattgtttccctcaagcgcatcatccgcttaaacaaaaaattcggacaggcgtttgccgggcgcatc

附录Bctcactccggaagagctgcttgaatttccgcaagcgggcaaacccgtcaactacctcgccaaacgctttgccgccaaagaagcctttgccaaagccgtcggcacgggcatacgcggcgcggtttccttccgcaacatcggcatcgggcatgacgcattgggcaagcccgaatttttctacggccccgccctgtccaaatggctggaggaacaaggcatcagccgcgtcagcctcagcatgagcgacgaagaagacaccgtattggcgtttgtcgttgccgaaaaataatgccgtctgaaatgcggcaaacccgttgacggcattgcccgtccctcatttgcactccgaccgaccaaccgcgtacccgccatgattcaagacacccgaccccttatccgcgtcgttgccggcatcctgctcgattcagacggcaactacctgctcagctcgcgccccgaaggcaaaccctatgccggatattgggaatttgccggcggcaaggtcgaagcgggcgaaaccgacttccaagccctgcaacgcgagtttgaagaagaactcggcatccgcatcctcgccgccacgccttggttgaccaaaatccattcctacgaacacgcccgcgtctgcctgaaattcctatgggtcaaccccgaccaatggacgggcaaaccgcaatcccgcgaagggcaggaatggtcttggcagaaggcgggtgattttaccgttgcccccatgctgcccgccaacggcgcgcttttgcgttcgctgtccgtcccgcgccgtttgtacggcagcctgaaaacgggtttgcacggagaaaacagtatgggcgcgtaccgcgtcctgcctttgggttcggcagagggaagcggtgcgaacgttttgatggaggcggcgcaatggcaggacagacccgaacacgccgacagcgtgtggatggtggtgcagacccgcgaacaatggcggcgggcgcaggaaaagggcgcggatgcggtcgtttggcgcgtgtgcgatgatgttcaggcacaagaggcggcagaagccctgcggcagggcgtatccgtgccgctcgtacttgcagcaaacggacagacggttgcacgttatggaaaactatggctcggattgggggcgcacgtggtggtaagggatgaaacaatagggaagaatcatgaataaaaaccgtaaattactgcttgccgcactgctgctgattgcctttgccgccgtcaagctcgttttgttgcaatggtggcaggcgcagcagccgcaagctgtggcggcgcaatgcgatttgaccgagggttgcacgctgccggacggaagccgcgtccgcgccgccgccgtttcaaccaaaaaaccgtttgatatttatatcgaacacgcgcccgccggcacggaacaggtcagcatcagcttcagtatgaaaaatatggatatgggtttcaaccgctatatgttcgagcggcaaccgtcggggacttggcaggcagtacgcatccgcctgcccatctgtgtcgaaggcaggcgcgattttacggcggacattacaatcggcagtcggacatttcagacggcatttaccgccgaataaacctttcaatccgccattgccggaacatccgtccggaaaggacacgttatgaatactttatatacacttttcgccacctgcccgcgcggcttggagaccgttttatctcaagaactcgaaagcctcggctgtaccgatgtacaagtgtttgacggcggcgtttcctgccggggcggattggaacaggtttacgccgccaacctgcattcgcgtactgccagccgtatcctgctgcgcctgaccaaagggacataccgcaatgagcgcgacatctacaaactcgccaaaaatatcaactggtttaattggtttactttacagcagacgttcaaagtcaaagtcgaggcaaagcgtgccaacgttaagagcatccaatttgtcggactgaccgtcaaagatgccgtctgcgacgctttccgcgacatttacgacgcacgtccgagcgtggacaaagccgcgcccgatgtccgcatccacgcctttttgaacgaacgcaatgtcgaaatctttattgacacttcgggcgaagccctgttcaaacgcggctaccgcctggataccggcgaagccccgctgcgcgaaaaccttgccgccggactgctgctctcggcaggctacgacggcacgcagccgtttcaagacccgttttgcggcagcggcacgattgctatcgaagccgcttggattgccgcccgccgcgcgccgggtatgatgcgccgtttcggttttgaaaaactgcaaaatttcgataaaacgctgtggtcggatttgcggcgccgcgccgaagcgcaaacccgccccgtccgcgccccgattgcaggcagcgacaacgaccgccgcatcgttcagacggcattggacaacgcacgccgcgccggggtggacgacatcgtttccttcagcgttgccgacgcgcagtccgtccgaccgaacggcgaaaacggcattatggtgtccaatccgccctacggcgtgcgccttgaggaagtccgcgccttgcaggcactgtatccgcagttggggacgtggttgaaaaaacattacgcaggctggttggcggcaatgtttaccggcgatagggaaatgcccaaattcatgtgcctgtcgcccaagcggaaaatcccgctttataacggcaacatcgactgccgcctgttcctgattgatatggtggaaggatcgaaccgttgaggaaagtgtacaaaaatgccgtctgaaaaatgttcagacggcatttatttttcggaatcaaccccgcttcaatacggatgtattgatgtagcgttggacacccgaggcaatggattgggcgcactgccggcggaaggattcgctgcccagcagcttctcttcggcaggattggacaggaaggcggtttcgaccaggatagacggcatatcgggtgcgcgcaaaacggcgaaattggcttcgtccaccctgcctttgtgcagatggttgagcctgcccaattcttcaagcaccagtttgccgagtttgcggctgtcgcgcagcgtggcggtttgggtcatgtcgagcagggcggtatcgacattgcggttgccgctggtcggtacgccgccgaccgcgtcggcattgttttgcgtctgttccaagaatttggcggcagagctggttgcgcctttggtgtttaacatataaacccccgtgccgcgcgcggaggggctggtgaaggcatcggcgtggatggagacaaatacgtccgcccgccgtgctcgccctttggcgacacgcacgcccaatgggatgaacacgtcttcgttgcgcgtcataaatacattgtaacctaatgcttccaactgatttttggtttccctggcaatggataggacgacatgtttttcctgtagaccgcccgggctgatggcgccggggtcttcaccgccgtgtcccggatcgagcatgatgacgggtctgcgcccgtttctgccgcgcccgggttggggcgtggtgttttgggcgaggtcggcttcgggagagccgcgcagggttttattcaggctaccgttgagcagtgccatcatcggatcgtcggcatccatcccgtgcggatagaggtcgacgacgaggcggttcttaaagccgccgacgggcggaagcgcgaagacttgtgcgtgggtgggctgtttcaaatcgatgacgaggcggacggtggtcggcgtgttctgacccgcgcgtatgctgcggataaaggggtcgtctgccatgactttctgagacagtccgtgcaatacggtattgatgttcgcgttttgtatgtcgacgaccagcctgcccgggttgtcgagcgtgaagtgctggtatttgagcgcggcggtgctttccagcgtcaggcgggtgtaggtgtgcgacggccatatccgtgcggcggtgaattgcggggcgcgtaccgttttggcaacggcggatgcgatggggcttagggcgaacagtgtgccggcggtgcggcggatgatttgtcttcgtgtcagtttgatcatagcggcaggctttcgcgtcctcgttcggtatgggcggtcagcaggcattttctgccgtcgccgtcgtgtgtcaatgttgcggtgatgtcggcgggcggcgtaaattccccgccctgttgcggccattcgatcaggcagacgctgtttgcggcaaacagttcgtcaagccccgcgtcttcccattcttcggggaacgagaagcggtagaggtcgaaatggtgcagggtgaagcgttccagcggataagattcgacgatggcgtaggtcggacttttgactgcgccctgatgacccaatccgcgcaggatgccgcgtgtcagcgtggttttgcccgcacccaaatccccttcgagataaatgaccagcggtgcgtttaaacgggaagaccacgccgcgcccaaatcgagtgtggcggcttcgtcggcaaggaatcgggagatagagggtaaatcagacatggaaacggtttgttgtaaggtctagggtattatgggcagttttgcaggttttgcaaactttgcacccgaggggcggatgcttcttgtccgagcattataacagccaaatccgcgttctgctttcagacggcaacggctgtcaagaaaaagcggcgcgtgtacaatacgcggattgtatgtttaggacggattggaaaaagaatggaaaatatcggcaggcagcgacccatcggcgtttttgactcgggaatcggcggtttgaccaatgtgcgagcgctgatggaacggctgccgatggagaacatcatttatttcggcgacacggcgcgcgtgccttacgggacgaaatctaaggcgaccatcgaaaatttctcgatgcagattgtcgattttttattggaacacgatgtcaaggcgatggttatcgcgtgcaatacgattgcggcggtggcggggcagaaaatccgtcaaaaaaccggcaatatgcccgttttggacgtgatttccgccggcgcgaaagccgcgctggcaacgacgcgcaacaataaaatcggcattatcgccaccaatacgacagtcaacagcaatgcttatgcgcgcgccatccataggaacaaccccgacacgctcgtccgcacgcaggccgcgccgctgctcgtccctttggtggaagagggctggctggaacacgaagttacccgcctgaccgtatgcgaatacctcaaaccattgcttgcagacggcatcgatacgctggtgttgggctgcacgcactttcccttgctcaagcccttaatcggcagggaggcgggcaatgtcgcgttggttgattctgcaattacaacggccgaagaaaccgcacgcgtccttgctcaggaaggattgctcaataccgacaacaacaatcccgactaccgtttttacgtcagcgatattcctttgaaattcagaaccatcggcgagcgttttctgggcaggacgatggagcagattgaaatggtgtctttgggttaaaacgatgacggaaagctgcccgagattacagaaacctaaaatcccgtcattcccacgaaagtgggaatctagacctgtcggtgcggaaacttatcggataaaacggtttctttagattttacgttctagattcccactttcgtgggaatgacgggattagagtttcaaaatttattctaaatagctgaagctcaacgcactggattcccgcctgcgcgggaatgacgaatttcaggtttctgtttttggttttctgtttttgtgaaaataacgggatttcagcttgtgggtatttaccggaaaaaacagaaaccgctccgccgtcattcccgcgcaggcgggaatctagacattcaatgctaaggcaatttatcgggaatgactgaaactcaaaaaactagattcccactttcgtgggaatgacggaatgtaggttcgtgggaatgacgggatgcaggtttccgtatggatggattcgtcattcccgagcagacgggatctagacattcaatgctaaggcaatttatcgggaatgactgaaactcaaaaaactagattcccactttcgtgggaatgacgggatataggtttccatgcggacgcgttcggattcacgactgcgcggaaatgacgggattttggtgtattccctaaaaaaataaaaaaacatttgcaactttgttaaaaataaaggctgtgttttaacgatgtgttgatatttaattttagaaaggtagctatttaatagttaccttttcttatttaaaaatagctttctcaaattccatgaacgcctcaatacgatatgcagatgctctatcgaaattaagtttcaacattttgtttattaaacattttattttagccatttttcaatatacccccaaatatacccccaatttgcacaagtcaaaattttaggcaggggttttgttgctcccatgatacgcgggcggatgccggcttttcgcgggggaaatacaaggggtctcggttcgggtgtcaaaatccctgtttcgtgttagtcatgtgggggggaagagggggttagaatgaagtaaagctgttgccctctccccgcaatagttccattag

附录Bgcgcggatgaatgaatagtttgtccctgccgatgacgatttcttgcagcacacctatgtctgaaagctctttcaggtacttagaggccgtctgccgtttggctatccctgccgcttctaggttggcaatgcgtgtatatggctgctcaaacagaagatttaccagttcgtgcgtgtagattccttgtgcgtgtgtccgtatgtgttgccgtgtctgctcgaacaggcggcgtatcgcatctattttcgataccgtccaatcggcggtgtcagctacgccgtctaagatgtagattatccagctttcccagtcctgccgttcggttacgcctaaaagcaggcggtaatagtccgccctgttttcgatgatgtagcggctcaaatacaaaataggcaaatccaaaagccctttttcaatcaatagcaggctgttcaatatgcgccccgtccgcccgttgccgtccgtaaacggatggatggcttcaaattggtaatgtgccgccgccatgatgataagcgggtctaaatcgccgctttcgtgaataaaccgctcccaatttgccagcttgccgcgtatggtttcttctccttcgggcggggtatagacaacatttccgctgttgcctccttttagggctgtgccgcctgttttgcggatggccatttcgtaggggtgcttgatggcgttgcagaccatgatggcggtttgtgtgcataaagggcggctcgtcagtgattcatagcctgcaaacagggcggtgcggtattgcagggcttctttcgtggcagggtcttgccgttccgtatccatttgcagggattgaaacagcttgtccgtggtggttacgatgttttcaatttccgaacttgcacgggcttccataacaggaagggtgttaatcagcatggcttgattcggtatcaattctgccgcctgctttaaacgggcaagggatgcacgggcggctatacaacgtttcaggatggttttgctttcaatatcctgttttggcggcaggggtggtaaatcgttatagggaatattgggtttccagttgctcatatttaaaatttcggaaaatttaaagatgtttccagtatatgtttacgccgtgtatatatcaaggatatatgtttaaaaatttggcttttgtaagtatatgggaggtaaaaccgcccgcaaaagtcaatttgcatggggttaattcaaaatccgcccgcccttcatctgcccgcttccctaaaaaaaccgtatatatataactgtccgcattctatcgctccggcgacgatacccatatttccaagtttgtgtatcaaaattgtatatcgggcatagactatttcggcgaggacgaagatatagatttccacgattgaatacatggaagccaagtacgtctatcaacactatattaaaacacagcctttttttttgaggtttcgggtaacttttaaaccgtcattcctacgaaaacagaaaatcaaaaacagaaatctcaaatcccgtcattcccgcgcaggcgggaatctagacattcaatgctaaggcaatttctcggaaatgactgaaactcaaaaaactggattcccactttcgtgggaatgacggaatgtaggttcgtgggaatgacgtggtgcaggtttccgtatggatggattcgtcattcccgcgcaggcgggaatctagacattcaatgctaaggcaatttatcgggaatgactgaaactcaaaaaactggattcccactttcgtgggaatgacgcgattagagtttcaaaatttattctaaatagctgaaactcaacgcactggattcccgcctgcgcgggaatgacgaagtggaagttacccgaaacttaaaacaagcgaaaccgaacgaactggattcccactttcgtgggaatgacggaatgcaggttcgtgggaatgacggaatgcaggttcgtgggaatgacgtagtgcaggtttccgtatggatggattcgtcattcccgcgcaggcgggaatctagacatgcaatgctaaggcaatttatcgggaatgactgaaactcaaaaaactggattcccgcctgcgcgggaatgacgaagtggaagttacccgaaacttaaaacaagcgaaaccgaacgaactggattcccactttcgtgagaatgacgggatgcaggttcgtgggaatgacgtggtgcaggtttccgtatggatggattcgtcattcccgcgcaggcgggaatctaggtctgtcggtgcggaaacttatcgggtaaaacggtttcttgagattttgcgtcttggattcccactttcgtgggaatgacgcgattagagtttcaaaatttattctaaatagctgaaactcaacgcactggattccgcctgcgcgggaatgacgaagtggaagttacccgaaacttaaaacaagcgaaaccgaacgaaccggattcccactttcgtgggaatgacgaatttcaggttactgtttttggttttctgtttttgtgaaaataatgggatttcagcttgtgggtatttaccggaaaaaacagaaaccgctccgccgtcattcccgcgcaggcgggaatctaggtctgtcggtgcggaaacttatcggataaaacggtttcttgagatttttcgtcctggattcccactttcgtgggaatgacgcgaacagaaaccgctccgccgtcattcccgcgcaggcgggaatctagacattcaatgctaaggcaatttatcgggaatgactgaaactcaaaaaactggattcccactttcgtgggaatgacgtggtgcaggtttccgtatggatggattcgtcattcccgcgcaggcgggaatctagaccttcaatactaaggcaatttatcggaaatgactgaaactcgaaaaactggattcccacttttgtgggaatgacgcgattagagtttcaaaatttattctaaatagctgaaactcaacacactggattcccgcctgcgcgggaatgacgaagtggaagttacccgaaacttaaaacaagcgaaaccgaacgaactggattcccactttcgtgggaatgacggaatgtaggttcgtgggaatgacggcggagcggtttctgctttttccaataaatgaccccaacttaaaatcccgtcattcccgcgcaggcgggaatctaggtctgtcggtgcggaaacttatcgggtaaaacggtttcttgagattttgcgtcctggattcccactttcgtgggaatgacggaatgtaggttcgtgggaatgacgggatataggtttccgtgcggacgcgttcggattcatgactgcgcgggaatgacgggattttggtgtattccctaaaaaaataaaaaagtatttgcaaatttgttaaaaataaataaaataataatccttatcattctttaattgaattggatttattatgaacaatccattggtgaatcaggctgctatggtgctgcctgtgtttttgttgagtgcttgtttgggcggaggcggcagtttcgatcttgattctgtcgataccgaagccccgcgtcccgcgccaaaatatcaagatgttttttccgaaaaaccgcaagcccaaaaagaccaaggcggatacggttttgcaatgaggttgaaacggaggaattggtatccgcaggcaaaagaagacgaggttaaactggacgagagtgattgggaggcgacaggattgccggacgaacctaaggaactccctaaacggcaaaaatcggttatcgaaaaagtagaaacagacagcgacaacaatatttattcttccccctatctcaaaccatcaaaccatcaaaacggcaacactggcaacggtataaaccaacctaaaaatcaggcaaaagattacgaaaattttaaatatgtttattccggctggttttacaaacacgccaaacgagagtttaacttaaaggtggaacctaaaagtgcaaaaaacggcgacgacggttatatcttctatcacggtaaagaaccttcccgacaacttcccgcttctggaaaaattacctataaaggtgtgtggcattttgcgaccgatacaaaaaagggtcaaaaatttcgtgaaattatccaaccttcaaaaagtcaaggcgacaggtatagcggattttcgggcgatgacggcgaagaatattccaacaaaaacaaatccacgctgacagatggtcaagagggttatggttttacctcaaatttagaagtggatttccataataaaaaattgacgggcaaactgatacgcaacaatgcgaataccgataacaaccaagccaccaccacgcaatactacagccttgaggctcaagtaacaggcaaccgcttcaacggcaaggcaacggcaaccgacaaaccccaacaaaacagcgaaaccaaggaacatccctttgtttccgattcgtcttctttgagcggcggctttttcggcccgcagggtgaggaattgggtttccgctttttgagcgacgatcaaaaagttgccgttgtcggcagcgcgaaaaccaaagacaaacccgcaaatggcaatactgcggcggcttcaggcggcacagatgcggcagcatcaaacggtgcggcaggcacgtcgtctgaaaacggtaagctgaccacggttttggatgcggtcgagctgaaattgggcgataaggaagtccaaaagctcgacaacttcagcaacgccgcccaactggttgtcgacggcattatgattccgctcttgcccgaggcttccgaaagtgggaacaatcaagccaatcaaggtacaaatggcggaacagcctttacccgcaaatttgaccacacgccggaaagtgataaaaaagacgcccaagcaggtacgcagacgaatggggcgcaaaccgcttcaaatacggcaggtgataccaatggcaaaacaaaaacctatgaagtcgaagtctgctgttccaacctcaattatctgaaatacggaatgttgacgcgcaaaaacagcaagtccgcgatgcaggcaggagaaagcagtagtcaagctgatgctaaaacggaacaagttgaacaaagtatgttcctccaaggcgagcgcaccgatgaaaaagagattccaagcgagcaaaacatcgtttatcgggggtcttggtacggatatattgccaacgacaaaagcacaagctggagcggcaatgcttccaatgcaacgagtggcaacagggcggaatttactgtgaattttgccgataaaaaaattactggtacgttaaccgctgacaacaggcaggaggcaacctttaccattgatggtaatattaaggacaacggctttgaaggtacggcgaaaactgctgagtcaggttttgatctcgatcaaagcaataccacccgcacgcctaaggcatatatcacagatgccaaggtgcagggcggtttttacgggcccaaagccgaagagttgggcggatggtttgcctatccgggcgataaacaaacgaaaaatgcaacaaatgcatccggcaatagcagtgcaactgtcgtattcggtgcgaaacgccaacagcctgtgcgataagcacggctgccgaacaatcaagaataaggcctcagacggcaccgctccttccgatgccgtctgaaagcgaagattagggaaacactatgcaacagcaacatttgttccgattcaatattttatgcctgtctttaatgactgcgctgcccgcttatgcagaaaatgtgcaagccggacaagcacaggaaaaacagttggataccatacaggtaaaagccaaaaaacagaaaacccgccgcgataacgaagtaaccgggctgggcaagttggtcaagtcttccgatacgctaagtaaagaacaggttttgaatatccgagacctgacccgttatgatccgggtattgccgtggtcgaacagggtcggggcgcaagttccggctattcaatacgcggcatggataaaaaccgcgtttccttaacggtggacggcgtttcgcaaatacagtcctacaccgcgcaggcggcattgggcgggacgaggacggcgggcagcagcggcgcaatcaatgaaatcgagtatgaaaacgtcaaagctgtcgaaatcagcaaaggctcaaactcggtcgaacaaggcagcggcgcattggcgggctcggtcgcatttcaaaccaaaaccgccgacgatgttatcggggaaggcaggcagtggggcattcagagtaaaaccgcctattccggcaaaaaccgggggcttacccaatccatcgcgctggcggggcgcatcggcggtgcggaggctttgctgatccacaccgggcggcgcgcgggggaaatccgcgcccacgaagatgcaggacgcggcgttcagagctttaacaggctggtgccggttgaagacagcagcaattacgcctatttcatcgttaaagaagaatgcaaaaacgggagttatgaaacgtgtaaagcgaatccgaaaaaagatgttgtcggcaaagacgaacgtcaaacggtttccacccgagactacacgggtcccaaccgcttcctcgccgatccgctttcatacgaaagccggtcgtggctgttccgcccgggttttcgttttgagaataagcggcactacatcggcggcatactcgaacacacgc

附录Baacaaactttcgacacgcgcgatatgacggttccggcattcctgaccaaggcggtttttgatgcaaataaaaaacaggcgggttctttgcccggtaacggcaaatacgcgggcaaccacaaatacggcggactgtttaccaacggcgaaaacggtgcgctggtgggcgcggaatacggtacgggcgtgttttacgacgagacgcacaccaaaagccgctacggtttggaatatgtctataccaatgccgataaagacacttgggcggattatgcccgcctctcttacgaccggcagggcatcggtttggataatcattttcagcagacgcactgttctgccgacggttcggacaaatattgccgcccgagtgccgacaagccgttttcctattacaaatccgatcgcgtgatttacggggaaagccacaggctcttgcaggcggcattcaaaaaatccttcgataccgccaaaatccgccacaacctgagcgtgaatctcgggtttgaccgctttggctctaatctccgccatcaggattattattatcaacatgccaaccgcgcctattcgtcgaacacgccccctcaaaacaacggcaaaaaaatcagccccaacggcagtgaaaccagcccctattgggtcaccataggcaggggaaatgtcgttacggggcaaatctgccgcttgggcaacaatacttatacggactgcacgccgcgcagcatcaacggtaaaagctattacgcggcagttcgggacaatgtccgtttgggcaggtgggcggatgtcggcgcgggcttgcgctacgactaccgcagcacgcattcggacgacggcagcgtttccaccggcacgcaccgcaccttgtcctggaacgccggcatcgtcctcaaacctaccgactggctggatttgacttaccgcacctcaaccggcttccgcctgccctcgtttgcggaaatgtacggctggcgggcgggtgttcaaagcaaggcggtcaaaatcgatccggaaaaatcgttcaacaaagaagccggcatcgtgtttaaaggcgatttcggcaacttggaggcaagttggttcaacaatgcctaccgcgatttgattgtccggggttatgaagcgcaaattaaagacggcaaagaagaagccaaaggcgacccggcttacctcaatgcccaaagcgcgcggattaccggcatcaatattttgggcaaaatcgattggaacggcgtatgggataaattgcccgaaggttggtattctacatttgcctataatcgtgtccgtgtccgcgacatcaaaaaacgcgcagaccgcaccgatattcaatcacatctgtttgatgccatccaaccctcgcgctatgtcgtcggcttgggctatgaccaaccggaaggcaaatggggtgtgaacggtatgctgacttattccaaagccaaggaaatcacagagttgttgggcagccgggctttgctcaacggcaacagccgcaatacaaaagccaccgcgcgccgtacccgcccttggtatattgtggacgtgtccggttattacacggttaaaaaacactttaccctccgtgcgggcgtgtacaacctcctcaactaccgctatgttacttgggaaaatgtgcggcaaactgccggcggcgcagtcaaccaacacaaaaatgtcggcgtttacaaccgatatgccgcccccggtcgcaactacacatttagcttggaaatgaagttctaaacgtccaaacgccgcaaatgccgtctgaaaggcttcagacggcattttttacacaatccccgccattttccatcatccccgatacaccgtaatctcgaaacccgtcattcccgcgcaggcgggaatccagtccgttcggtttcggtttttttgaggtttcgggtaacttctaaaccgttattcccgcgaaaacagaaaatcaaaaacagaaacctcaaatcccgttattcccgagcagacgggatctagggcgtaaaatctaaagaaaccgttttatccgataagtttccgcaccgacagactagattcccgcctgcgcgggaatgacgttatatttttcgcatttgataaaaaagaccgtttgaaattttttcagcggacgcaaagtattgcgtaaaatgctgcttataagaaacgcaaggcgggcgtaggatgtgcagcgtggacatagggctggctgtagggtgggcttcagcccaccaatcccaccgtttccacctattcccccaactccgtcaatgttatccattccgcccattcccaccgaaaaccgaaaccgccgtattcccaaaaacctttgatgcggtgaaattggtgggctgaagcccaccctacagcccaccctacggctcgccgaaatttcgtcattcccgcgcaggcgggaatccaggtctgtcggtgcggaaacttatcggataaaacggtttcttgagattttacgtcctagattcccacttccgtgggaatgacgggatgcaggttttcgtgcggacgcgttcggattcacgactgcgcgggaatgacgggatttcagtttgcggattgatttgaagttgcaaaatcccaacggatcggattaccgctttcgcgtttcaaagttacggcgttatcggaaaaacagaaaatcaaagctgcaagaatttatttaaaacaaccgaatttcaacggatcggattctcgcctgtagggaatgacggcggaaggttttttgtcttttctgacagatgtccgcaatctgaaatcctgaccgtgggaacgacggtatagtggattaacaaaaaccagtacggcgttggctcgccttagctcaaagagaacgattctctaaggtgctgaagcaccaagtgaatcggttccgtactatttgtactgtctgcggctttgtcgccttgtcctgatttttgttaatccactatataaatatttctatttcaatccaatataaaatgccgtccgaacatcgttcggacggcatttttaatgcttcaaatcagttggtgccgactttgattttctgccacaggctgacagacagttttttcgcatccgtgctcatttgcggcatcacgaaaccgtctttcatatcctgctcgttcgggaagatggaacgggtgttcaccagctcggcaggcattttttcgcgcgccggtttgctggcgggggcaaaggttacggcgatgccgtttttcgccgcgatttcggggtcgagcgtgtagttgatgtatttgtgggcattggcgacgtttttcgcatcggcgggaatcagccaagactcaatccagaagcccatacctttcggtgtcagcacttcgatgccgacgttgtttttcacttcctcggaacgtgctttcgccaagttcaaatcgccgccgttgcctgccgccaggcagatgtcgccgcgtgccagctcgtcgatgatggacgggctgaaacgtttgacatccggacggatagacttcaacacttccgccgccgccttcaagtcttcaggattcgagcctttggggtctttgcccaagtagttcagcaaaatcgggaacatttcactcggggtgtcccacagggcgatgccgcaggatttcagcttgtgggtgtattcgggtttgaacagcaaatcccagccgttttcgggcagcttgccgcccaaaagctctttgcccttcgccgtaatcgcaatcgtgttcacgccggagaaataggggacggcatactggttgcccgggtcggcggtttccagcattttcaagagttcgggatcgatgtttttatagttgggaatcaggtctttgttgactttttgatacgcgcccgcctcgatttggcgcggcaggaaggcgatgcccggcacgaccaaatcgtaaccggatttgccggtcagcattttggcttccagcgtttcattgttttcgtacaagtcgtaagtcagcttcagattgttggcttttttaaagtcttcgaccgtactctcatcaacatagttcgaccagttgtagatgttcagagtatcggtggcagcggcttcggcattggcagcagacgcagcgtctgcttgaggttgcacggcgtttttttcgctgccgccgcaggctgccagagacagcgcggccaaaacggctaatacggattttttcatacgggcagattcctgatgaaagaggttggaaaaaaagaaatccccgcgccccatcgttaccccggcgcaaggtttgggcattgtaaagtaaatttgtgcaaactcaaagcgatattggactgattttcctaaaaaattatcctgtttccaaaaggggagaaaaacgtccgcccgattttgccgtttttttgcgctgtcagggtgtccgacgggcggatagagagaaaaggcttgcatataatgtaaaccccctttaaaattgcgcgtttacagaatttatttttcttccaggagattccaatatggcaaacagcgcacaagcacgcaaacgtgcccgccagtccgtcaaacaacgcgcccacaatgctagcctgcgtaccgcattccgcaccgcagtgaaaaaagtattgaaagcagtcgaagcaggcgataaagctgccgcacaagcggtttaccaagagtccgtcaaagtcatcgaccgcatcgccgacaagggcgtgttccacaaaaacaaagcggcacgccacaaaagccgtctgtctgcaaaagtaaaagccttggcttgatttttgcaaaaccgccaaggcggttgatacgcgataagcggaaaaccctgaagcccgacggtttcggggttttctgtattgcgggggcaaaatcccgaaatggcggaaagggtgcgattttttatccgaatccgctataaaatgccgtttgaaaaccaatatgccgacaatgggggcggagatgaatacacggaatatgcgctatattcttttgacaggactgttgccgatggcatccgcttttggagagaccgcgctgcaatgcgccgctttgacggacaatgttacgcgtttggcgtgttacgacaggatttttgcggcacagcttccgtcttcggcagggcaggaagggcaggagtcgaaagccgtactcaatctgacggaaaccgtccgcagcagcctggataagggcgaggcggtcattgttgttgaaaaaggcggggatgcgcttcctgccgacagtgcgggcgaaaccgccgacatctatacgcctttgagcctgatgtacgacttggacaaaaacgatttgcgcgggctgttgggcgtacgcgaacacaatccgatgtaccttatgccgctctggtacaacaattcgcccaactatgccccgggttcgccgacgcgcggtacgactgtacaggaaaaattcggacagcagaaacgtgcggaaaccaaattgcaggtttcgttcaaaagcaaaattgccgaagatttgtttaaaacccgcgcggatctgtggttcggctacacccaaagatccgattggcagatttacaaccaaggcaggaaatccgcgccgttccgcaatacggattacaaacctgaaattttcctgacccagcctgtgaaggcggatttgccgttcggcggcaggctgcgtatgctcggtgcgggttttgtccaccagtccaacggacagagccgtcccgaatcgcgttcgtggaacaggatttacgccatggcaggcatggaatggggcaaattgacggtgattccgcgcgtgtgggtgcgtgcgttcgatcagagcggcgataaaaacgacaatcccgatattgccgactatatggggtatggcgacgtgaagctgcagtaccgcctgaacgacaggcagaatgtgtattccgtattgcgctacaaccccaaaacgggctacggcgcgattgaagccgcctacacgtttccgattaagggcaaactcaaaggcgtggtacgcggattccacggttacggcgagagcctgatcgactacaaccacaagcagaacggtatcggtatcgggttgatgttcaacgacttggacggcatctgaaccgcgtgttcagacggtatatcaagttgccgtgccgtctgaagccgccggcggtttggcggggcgggataaaaatttcgtttgaatccgggcaaacgcgtataatgtgcggtttgtacggactttgtcggaatcaagcaagatgacggaacctgcggccgaaggcggcaaagctgccaaggcgttaaaaaaatatctgattacgggcattttggtctggctgccgattgcggtaacggtttgggtggtttcctatatcgtttccgcgtccgatcagctcgtcaacctgctgccgaagcaatggcggccgcaatatgttttggggtttaatatcccggggctgggcgttatcgttgccattgccgtattgtttgtaaccggattgtttgccgccaacgtattgggtcggcagatcctcgccgcgtgggacagcctgttggggcggattccggttgtgaaatccatctattcgagtgtgaaaaaagtatccgaatcgctgctgtccgacagcagccgttcgtttaaaacgccggtactcgtgccgtttccccagcccggtatttggacgattgctttcgtgtcagggcaggtgtcgaatgcggttaaggccgcattgccgaaggacgg

附录Bcgattatctttccgtgtatgttccgaccacgccgaatccgaccggcggttactatattatggtaaagaaaagcgatgtgcgcgaactcgatatgagcgtggacgaagcattgaaatatgtgatttcgctgggtatggtcatccctgacgacctgcccgtcaaaacattggcaggacctatgccgtctgaaaaggcggatttgcccgaacaacaataaagccgccgttcagacggcattttctgttttcagtttaaatcaataaaaggtgattttatgcgtaccaactattgcggcctgatcagtgagcaatacttagaccaaaccgttaccgtcaaaggctgggtacaccgtcgacgcgaccacggcggtgtgatttttatcgacctgcgcgaccgcgaaggcatcgtccaagtcgtgatcgatcccgacacgcccgaagcgtttgccgctgccgattcctcccgcaacgaatacgttttgagcattaccggccgcgtacgcaa