CN101032223A - Tissue cultivating method of spiceberry melaleuca alternifolia - Google Patents

Tissue cultivating method of spiceberry melaleuca alternifolia Download PDF

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Publication number
CN101032223A
CN101032223A CN 200610037340 CN200610037340A CN101032223A CN 101032223 A CN101032223 A CN 101032223A CN 200610037340 CN200610037340 CN 200610037340 CN 200610037340 A CN200610037340 A CN 200610037340A CN 101032223 A CN101032223 A CN 101032223A
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culture
explant
tea tree
inducing
tissue culture
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CN 200610037340
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CN101032223B (en
Inventor
赖桂星
林良科
石斗开
朱文辉
陈文炳
王建红
郭逸成
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Yongquan Science & Technology Co Ltd Xiamen
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Yongquan Science & Technology Co Ltd Xiamen
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Abstract

The present invention discloses tissue culture process of alternate leaf cajeputtree as one kind of perfume plant. The tissue culture process includes the following steps: cutting axillary bud as the explant from the mother body, washing and sterilizing; the subsequent inducing culturing in inducing culture medium comprising 7/10MS-MS, 6-BA 0.1-0.3 mg/L and NAA 0.1-0.3 mg/L; secondary culturing in secondary culture medium comprising 7/10 MS-MS, 6-BA 0.4-0.6 mg/L and NAA 0.05-0.15 mg/L; and final rooting culturing in rooting culture medium comprising 2/5MS -3/5MS, and IBA 0.5-0.7 mg/L. The tissue culture process has the culturing conditions including illumination intensity of 2500-3000Lux, and temperature of 26+/-1 deg.c. The tissue culture process is suitable for fast propagation in industrial scale.

Description

The method for tissue culture of aromatic plant narrow leaved tea tree
Technical field
The invention belongs to biological technical field, specifically refer to the method for tissue culture of aromatic plant narrow leaved tea tree.
Background technology
Narrow leaved tea tree (Melaleuca altennifolia) is the Myrtaceae paperbark, originates in Australia, is a kind of evergreen dungarunga, is suitable for South China's popularizing planting in China.The tea oil that its fresh branches and leaves extract has disinfectant character, can suppress many ubiquitous pathogenic cells and mould effectively, is widely used in industries such as medicine, food, anticorrosion, cosmetic, eyeshield and health products.But, be difficult to satisfy in a short time the growing market demand with conventional propagation method because provenance lacks, the cuttage difficulty is taken root, breeding cycle is long and genetic variation etc. is all multifactor.
Plant Tissue Breeding (Tissue culture) is meant the totipotency of utilizing plant cell, (part), tissue and cell exteriorize in the plant corpus, physiological environment in the analogue body, under aseptic, proper temperature and certain condition of culture, make it existence, growth, keep the method for its normal configuration and function.
The inventor finds after deliberation, if adopt method for tissue culture, both can keep the merit of parent, effectively saves production cost, and can alleviate the imbalance between supply and demand of present narrow leaved tea tree nursery stock again.This case is arisen spontaneously.
Summary of the invention
The present invention aims to provide a kind of method for tissue culture of aromatic plant narrow leaved tea tree, thereby realizes the commercialization fast breeding.
In order to realize the foregoing invention purpose, technical scheme of the present invention is:
The method for tissue culture of aromatic plant narrow leaved tea tree, the clip axillalry bud is as explant from the narrow leaved tea tree parent with merit, earlier explant is cleaned, sterilizes, insert inducing culture then and carry out inducing culture, the inducing culture proportioning is: 7/10MS-MS (Marashige ﹠amp; Sroog) conventional minimal medium, 6-BA (6-Bian Ji purine) 0.1-0.3mg/L, NAA (methyl) 0.1-0.3mg/L; Insert subculture medium again and carry out successive transfer culture, the subculture medium proportioning is: 7/10MS-MS, 6-BA0.4-0.6mg/L, NAA0.05-0.15mg/L; At last, enter the culture of rootage stage, the root media proportioning is: 2/5MS-3/5MS, IBA (indolebutyric acid) 0.5-0.7mg/L; The condition of above inducing culture, successive transfer culture, culture of rootage is: intensity of illumination is 2500-3000Lux, and temperature is 26 ± 1 ℃.
The concrete steps that above-mentioned explant cleans, sterilizes are: use detergent immersion 10 minutes earlier, place under the clear water and washed 30 minutes; With alcohol-pickled 20 seconds of 75%, aseptic water washing 2 times was used 0.1%HgCl again 2Sterilized aseptic water washing 5 times 7 minutes.
After above-mentioned explant cleaned, sterilizes, suck dry moisture was cut to the stem section of 1 ± 0.1cm, inserted inducing culture then and carried out inducing culture.
After adopting said method; method for tissue culture of the present invention has solved narrow leaved tea tree seminal propagation and has gone out long, problems such as survival rate is low, production cost height of garden cycle; realize the commercial scale fast breeding; the propagation multiplying power reached 4.6 times in 30 days; rooting rate is near 100%; transplanting survival rate has solved the root problem of nursery stock demand more than 96% for large tracts of land popularizing planting narrow leaved tea tree.
Embodiment
Utilization the present invention carries out the specific embodiment of the tissue culture of aromatic plant narrow leaved tea tree.
A, explant are handled and inducing culture
The clip axillalry bud cleans, sterilizes explant earlier as explant from the narrow leaved tea tree parent with merit, and concrete steps are with detergent immersion 10 minutes, place clear water under and wash 30 minutes; With alcohol-pickled 20 seconds of 75%, aseptic water washing 2 times was used 0.1%HgCl again 2Sterilized 7 minutes, aseptic water washing 5 times, suck dry moisture are cut to the stem section about 1cm, and induce differentiation culture on the inducing culture, condition of culture being inoculated on the superclean bench then: intensity of illumination is: 2500-3000Lux, temperature is 26 ± 1 ℃.The inducing culture proportioning is: 7/10MS-MS, 6-BA0.1-0.3mg/L, NAA0.1-0.3mg/L.
B, successive transfer culture
At inducing culture about about 30 days, when treating the high 3-4cm of axillalry bud, can enter successive transfer culture, condition of culture is identical with inducing culture, and the proliferated culture medium proportioning is: 7/10MS-MS, 6-BA0.4-0.6mg/L, NAA0.05-0.15mg/L, the propagation multiplying power reaches 4.6 times.
C, culture of rootage
At successive transfer culture about 25 days, height of seedling just can carry out culture of rootage when 1.0CM is above, condition of culture is identical with above cultivation, the root media proportioning is: 2/5MS-3/5MS, IBA (indolebutyric acid) 0.5-0.7mg/L, about about 20 days of culture of rootage, rooting rate is near 100%, and root is about 1.5CM, height of seedling 3-4CM, just can transplant by bottle outlet, transplanting survival rate is suitable for industrial production requirement fully more than 96%.

Claims (3)

1, the method for tissue culture of aromatic plant narrow leaved tea tree is characterized in that:
The clip axillalry bud cleans, sterilizes explant earlier as explant from the narrow leaved tea tree parent;
Insert inducing culture then and carry out inducing culture, the inducing culture proportioning is: 7/10MS-MS, 6-BA0.1-0.3mg/L, NAA0.1-0.3mg/L;
Insert subculture medium again and carry out successive transfer culture, the subculture medium proportioning is: 7/10 MS-MS, 6-BA0.4-0.6mg/L, NAA0.05-0.15mg/L;
At last, enter the culture of rootage stage, the root media proportioning is: 2/5 MS-3/5MS, IBA0.5-0.7mg/L;
The condition of above inducing culture, successive transfer culture, culture of rootage is: intensity of illumination is 2500-3000Lux, and temperature is 26 ± 1 ℃.
2, the method for tissue culture of aromatic plant narrow leaved tea tree according to claim 1 is characterized in that: the concrete steps that explant cleans, sterilizes are earlier with detergent immersion 10 minutes, place clear water under and wash 30 minutes; With alcohol-pickled 20 seconds of 75%, aseptic water washing 2 times was used 0.1%HgCl again 2Sterilized aseptic water washing 5 times 7 minutes.
3, the method for tissue culture of aromatic plant narrow leaved tea tree according to claim 1, it is characterized in that: after explant cleaned, sterilizes, suck dry moisture was cut to the stem section of 1 ± 0.1cm, inserted inducing culture then and carried out inducing culture.
CN2006100373407A 2006-08-24 2006-08-24 Tissue cultivating method of spiceberry melaleuca alternifolia Expired - Fee Related CN101032223B (en)

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Application Number Priority Date Filing Date Title
CN2006100373407A CN101032223B (en) 2006-08-24 2006-08-24 Tissue cultivating method of spiceberry melaleuca alternifolia

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CN101032223B CN101032223B (en) 2011-04-20

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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102342232A (en) * 2010-08-04 2012-02-08 湖南康普医药研究院 Eucalyptus type melaleuca alternifolia seedling raising and planting method
CN102577942A (en) * 2011-01-06 2012-07-18 厦门涌泉集团有限公司 Tissue culture method of callistemon hybridus 'golden ball'
CN102870675A (en) * 2012-09-26 2013-01-16 李斌 Method for tissue culture of sprout
CN103430655A (en) * 2012-09-10 2013-12-11 中国科学院华南植物园 Method for increasing seed germination rate and survival rate of setaria plicata
CN104365318A (en) * 2014-09-29 2015-02-25 梁彩英 Standardized myrtle planting technology
CN104429941A (en) * 2014-10-24 2015-03-25 杨业容 In-vitro rapid propagation technique of melaleuca alternifolia
CN105340749A (en) * 2015-11-27 2016-02-24 肇庆学院 Method for sterile cottage and rapid seedling propagation and raising of Australian melaleuca alternifolia
CN105613293A (en) * 2016-01-15 2016-06-01 广西壮族自治区林业科学研究院 4-terpilenol-type melaleuca alternifolia tissue culture subculture multiplication method and culture medium thereof
CN105706927A (en) * 2016-02-04 2016-06-29 广西壮族自治区林业科学研究院 Open rooting culture method for 4-terpilenol melaleuca alternefolia tissue culture subculture buds
CN105766633A (en) * 2016-03-09 2016-07-20 广西壮族自治区林业科学研究院 Non-tube rooting method for tissue culture proliferation buds of melaleuca alternifolia
CN106416680A (en) * 2016-09-08 2017-02-22 广西壮族自治区林业科学研究院 Melaleuca alternifolia cutting propagation method
CN108651283A (en) * 2018-05-17 2018-10-16 邵武市闽澳农业发展有限公司 A kind of narrow leaved tea tree tissue-cultured seedling culture of rootage method
CN115299345A (en) * 2022-09-05 2022-11-08 中国科学院华南植物园 One-step tissue culture method for melaleuca alternifolia

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102342232A (en) * 2010-08-04 2012-02-08 湖南康普医药研究院 Eucalyptus type melaleuca alternifolia seedling raising and planting method
CN102577942A (en) * 2011-01-06 2012-07-18 厦门涌泉集团有限公司 Tissue culture method of callistemon hybridus 'golden ball'
CN103430655A (en) * 2012-09-10 2013-12-11 中国科学院华南植物园 Method for increasing seed germination rate and survival rate of setaria plicata
CN103430655B (en) * 2012-09-10 2015-05-20 中国科学院华南植物园 Method for increasing seed germination rate and survival rate of setaria plicata
CN102870675A (en) * 2012-09-26 2013-01-16 李斌 Method for tissue culture of sprout
CN104365318A (en) * 2014-09-29 2015-02-25 梁彩英 Standardized myrtle planting technology
CN104429941A (en) * 2014-10-24 2015-03-25 杨业容 In-vitro rapid propagation technique of melaleuca alternifolia
CN105340749A (en) * 2015-11-27 2016-02-24 肇庆学院 Method for sterile cottage and rapid seedling propagation and raising of Australian melaleuca alternifolia
CN105613293A (en) * 2016-01-15 2016-06-01 广西壮族自治区林业科学研究院 4-terpilenol-type melaleuca alternifolia tissue culture subculture multiplication method and culture medium thereof
CN105706927A (en) * 2016-02-04 2016-06-29 广西壮族自治区林业科学研究院 Open rooting culture method for 4-terpilenol melaleuca alternefolia tissue culture subculture buds
CN105766633A (en) * 2016-03-09 2016-07-20 广西壮族自治区林业科学研究院 Non-tube rooting method for tissue culture proliferation buds of melaleuca alternifolia
CN105766633B (en) * 2016-03-09 2018-06-01 广西壮族自治区林业科学研究院 A kind of narrow leaved tea tree tissue culture subculture bud outside sprout-cultivating-bottle method
CN106416680A (en) * 2016-09-08 2017-02-22 广西壮族自治区林业科学研究院 Melaleuca alternifolia cutting propagation method
CN108651283A (en) * 2018-05-17 2018-10-16 邵武市闽澳农业发展有限公司 A kind of narrow leaved tea tree tissue-cultured seedling culture of rootage method
CN115299345A (en) * 2022-09-05 2022-11-08 中国科学院华南植物园 One-step tissue culture method for melaleuca alternifolia
CN115299345B (en) * 2022-09-05 2023-06-16 中国科学院华南植物园 One-step tissue culture method for melaleuca alternifolia

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