CN101024850A - Chitosan oligosaccharide/chito-oligomer single enzymatic production process - Google Patents
Chitosan oligosaccharide/chito-oligomer single enzymatic production process Download PDFInfo
- Publication number
- CN101024850A CN101024850A CNA2006101665949A CN200610166594A CN101024850A CN 101024850 A CN101024850 A CN 101024850A CN A2006101665949 A CNA2006101665949 A CN A2006101665949A CN 200610166594 A CN200610166594 A CN 200610166594A CN 101024850 A CN101024850 A CN 101024850A
- Authority
- CN
- China
- Prior art keywords
- production process
- oligochitosan
- enzyme
- chitosan
- chitooligosaccharide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 23
- 230000002255 enzymatic effect Effects 0.000 title claims description 9
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 title 1
- 229920001661 Chitosan Polymers 0.000 claims abstract description 64
- 108090000790 Enzymes Proteins 0.000 claims abstract description 11
- 102000004190 Enzymes Human genes 0.000 claims abstract description 11
- 238000006243 chemical reaction Methods 0.000 claims abstract description 8
- 150000001768 cations Chemical class 0.000 claims abstract description 7
- 239000011347 resin Substances 0.000 claims abstract description 7
- 229920005989 resin Polymers 0.000 claims abstract description 7
- 108010093096 Immobilized Enzymes Proteins 0.000 claims abstract description 6
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical class [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims abstract description 5
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 12
- 239000000047 product Substances 0.000 claims description 12
- 238000001976 enzyme digestion Methods 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 7
- 238000004587 chromatography analysis Methods 0.000 claims description 6
- 230000035484 reaction time Effects 0.000 claims description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 4
- 238000000746 purification Methods 0.000 claims description 4
- 238000009288 screen filtration Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 239000007857 degradation product Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 12
- 230000015556 catabolic process Effects 0.000 abstract description 3
- 238000006731 degradation reaction Methods 0.000 abstract description 3
- 101710136247 Endo-chitosanase Proteins 0.000 abstract 1
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 238000011031 large-scale manufacturing process Methods 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 108010089807 chitosanase Proteins 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000003912 environmental pollution Methods 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 3
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 3
- 229960002442 glucosamine Drugs 0.000 description 3
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 238000002144 chemical decomposition reaction Methods 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000000543 intermediate Substances 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000010409 thin film Substances 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 108010064696 N,O-diacetylmuramidase Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN200610166594A CN100584955C (en) | 2006-12-27 | 2006-12-27 | Chitosan oligosaccharide/chito-oligomer single enzyme production process |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN200610166594A CN100584955C (en) | 2006-12-27 | 2006-12-27 | Chitosan oligosaccharide/chito-oligomer single enzyme production process |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101024850A true CN101024850A (en) | 2007-08-29 |
CN100584955C CN100584955C (en) | 2010-01-27 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN200610166594A Active CN100584955C (en) | 2006-12-27 | 2006-12-27 | Chitosan oligosaccharide/chito-oligomer single enzyme production process |
Country Status (1)
Country | Link |
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CN (1) | CN100584955C (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101619082B (en) * | 2008-07-01 | 2011-08-10 | 中国科学院大连化学物理研究所 | Method for separating and purifying chitosan oligosaccharide monomer |
CN102994489A (en) * | 2012-10-09 | 2013-03-27 | 山西农业大学 | Biotin-avidin system immobilized glucoamylase and preparation method thereof |
CN105861597A (en) * | 2016-06-01 | 2016-08-17 | 青岛科海生物有限公司 | Novel marine polysaccharide-chitooligosaccharide production technique |
CN111171182A (en) * | 2020-03-02 | 2020-05-19 | 江西师范大学 | Method for preparing chitosan oligosaccharide monomer by modifying chitosan oligosaccharide with aromatic aldehyde |
CN113293185A (en) * | 2021-04-02 | 2021-08-24 | 上海应用技术大学 | Preparation method of chitosan oligosaccharide |
CN117238447A (en) * | 2023-11-16 | 2023-12-15 | 山东卫康生物医药科技有限公司 | Medical functional food production monitoring and adjusting system |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1300311C (en) * | 2005-06-14 | 2007-02-14 | 浙江大学 | Preparation method of chitin incision enzyme |
CN1320124C (en) * | 2005-09-15 | 2007-06-06 | 武汉大学 | Preparation of low-molecular weight chitoglycan or chitooligose |
-
2006
- 2006-12-27 CN CN200610166594A patent/CN100584955C/en active Active
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101619082B (en) * | 2008-07-01 | 2011-08-10 | 中国科学院大连化学物理研究所 | Method for separating and purifying chitosan oligosaccharide monomer |
CN102994489A (en) * | 2012-10-09 | 2013-03-27 | 山西农业大学 | Biotin-avidin system immobilized glucoamylase and preparation method thereof |
CN102994489B (en) * | 2012-10-09 | 2014-01-08 | 山西农业大学 | Biotin-avidin system immobilized glucoamylase and preparation method thereof |
CN105861597A (en) * | 2016-06-01 | 2016-08-17 | 青岛科海生物有限公司 | Novel marine polysaccharide-chitooligosaccharide production technique |
CN111171182A (en) * | 2020-03-02 | 2020-05-19 | 江西师范大学 | Method for preparing chitosan oligosaccharide monomer by modifying chitosan oligosaccharide with aromatic aldehyde |
CN113293185A (en) * | 2021-04-02 | 2021-08-24 | 上海应用技术大学 | Preparation method of chitosan oligosaccharide |
CN117238447A (en) * | 2023-11-16 | 2023-12-15 | 山东卫康生物医药科技有限公司 | Medical functional food production monitoring and adjusting system |
Also Published As
Publication number | Publication date |
---|---|
CN100584955C (en) | 2010-01-27 |
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Effective date of registration: 20161026 Address after: 362011 Room 606, B building, East, Anji Road, Luojiang District, Fujian, China Patentee after: Quanzhou kserp Biological Technology Co.,Ltd. Address before: The east of the road, Fengze District, Quanzhou City, Erythrina Fujian province 362000 evening Quanzhou Huilong group building ten floor Patentee before: Huilong group (Quanzhou) Industrial Investment Ltd. |
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Address after: 362011 6th floor, west side of building 3, Chengda Industrial Park, Xunmei Industrial Zone, Chengdong street, Fengze District, Quanzhou City, Fujian Province Patentee after: Quanzhou kserp Biological Technology Co.,Ltd. Address before: 362011 Room 606, building B, Shangdong international, Anji Road, Luojiang District, Quanzhou City, Fujian Province Patentee before: Quanzhou kserp Biological Technology Co.,Ltd. |
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