CN101006791A - A biocontrol strains against water hyacinth and its use - Google Patents

Abstract

The invention discloses the usage of Myrothecium roridum, and also discloses a compound pesticide of Myrothecium roridum. Myrothecium roridum has strong and quick special pathopoiesis to Eichhornia crassipes but no pathopoiesis to many main crops, and has high safety.

Description

A kind of water hyacinth biological control bacterial strain and its application

technical field

The invention relates to a microorganism-derived biological inhibitor, and more specifically, the invention relates to a new bacterial strain, which can be applied to inhibit the growth of water hyacinth, a malignant aquatic weed.

Background technique

Water hyacinth is a common name for water hyacinth (Eichhornia crassipes). Water hyacinth is native to Brazil and has been introduced to all over the world as an ornamental plant because of its beautiful flowers. However, due to its flooding, it has seriously affected the waterway and water quality, and has become one of the top ten harmful weeds in the world.

Water hyacinth was introduced into my country as a flower in 1901. It was popularized and planted as pig feed in the 1950s and 1960s, and then became wild, which eventually led to the flooding of rivers and lakes in the south in recent years. Water hyacinth has now become one of the 16 invasive alien species announced by the State Environmental Protection Administration in 2003. From January to August 2002, there were about 1.84 million tons of water hyacinth in Shanghai. A large amount of manpower, material resources and funds have been invested in water hyacinth management, but the problem of water hyacinth cannot be completely solved.

The current international management of water hyacinth includes biological control of weevils, temporary use of chemical herbicides, and manual/mechanical fishing. The biological control of weevil can inhibit the growth of water hyacinth population in some areas, but the damage of water hyacinth cannot be fundamentally controlled. In addition, because it is an alien species, the biological safety or risk of introducing a new species is worth exploring and studying. Water hyacinth can be effectively killed by chemical herbicides, but they should only be used with caution, temporarily and during large-scale outbreaks when other methods are ineffective. The first reason is that a large number of chemical herbicides will cause new pollution to the water body, and the second is that the herbicides have a serious impact on other aquatic plants. Manual and mechanical methods not only require a large amount of manpower, but also require a large amount of funds, but are still the main methods for controlling water hyacinth at present.

At present, the international community has begun to pay attention to and strengthen the research on pathogenic bacteria of water hyacinth, expecting to carry out biological control of water hyacinth through microbial preparations. According to reports, as of 1996, there were 36 genera and 60 species of pathogenic fungi on water hyacinth worldwide, of which 54 pathogenic fungi were found in non-origin countries, among which Taiwan and Hong Kong in China A pathogenic fungus was reported. The Center for Aquatic and Alien Organisms, Department of Plant Diseases, University of California, USA reported in 2001 that five pathogenic fungi such as Acremonium zonatum, Alternariaeichhorniae, and Cercospora piaropi are the most promising pathogen species for biocontrol herbicides.

The introduction of alien species, especially the introduction of microorganisms, is subject to particularly strict restrictions in countries all over the world due to the great risk, and our country is no exception. Therefore, there is an urgent need to find and study a safe, stable and efficient biological control agent, so as to provide a new way for establishing a safe and effective water hyacinth management system.

Contents of the invention

The purpose of the present invention is to provide a new microorganism that can effectively inhibit the growth of water hyacinth.

The object of the present invention is also to provide a biological inhibitor that can effectively prevent and control the growth and spread of water hyacinth.

In one aspect of the present invention, there is provided a use of the Mycobacterium dew varnish or its spores for preparing a preparation for inhibiting the growth of water hyacinth.

In a preferred example of the present invention, the said Mycobacterium dewwet is the No. CCTCC No. M205120.

In the second aspect of the present invention, there is provided a pesticide composition, which contains an effective amount of the above-mentioned Myrophyllum dew spp. or its spores, and the balance of a pesticide acceptable carrier.

In a preferred example of the present invention, the pesticide composition is a dosage form selected from the group consisting of wettable powder, emulsifiable concentrate, aqueous solution, emulsion, sprayable solution, oily or aqueous dispersion, suspension, powder , granules, or microcapsules.

In another preferred example of the present invention, the pesticide composition is in the form of a sprayable solution.

In another preferred embodiment of the present invention, the pesticide composition contains 10 ⁴ -10 ¹² Myromyces dew varnish or its spores per gram (or milliliter) of the composition.

In another preferred embodiment of the present invention, the pesticide composition contains 10 ⁶ -10 ¹⁰ Myromyces dew varnish or its spores per gram (or milliliter) of the composition.

In another preferred embodiment of the present invention, the composition further contains substances selected from the group consisting of surfactants, spreading agents, filling substances, or germ activity accelerators.

In another preferred example of the present invention, the pesticide composition contains

Mycobacterium dew varnish or its spores: 10 ⁴ -10 ¹² /mL;

Surfactant: 0.001-0.01% (V/V); and

The balance is water.

In another preferred example, in the pesticide composition, the said Myromyces varnish or its spores are: 10 ⁵ -10 ⁹ /ml; more preferably, the said For: 10 ⁶ -10 ⁸ /ml.

In another preferred example, the surfactant includes, but is not limited to: Tween 20 or NP-40.

In the third aspect of the present invention, there is provided a method for inhibiting the growth of water hyacinth, the method comprising: applying the above-mentioned Mycobacterium dewwetch or its spores on water hyacinth.

In another preferred example, the application rate is 10 ⁵ -10 ¹² /m ² water hyacinth; more preferably, the application rate is 10 ⁶ -10 ¹⁰ /m ² water hyacinth.

In the fourth aspect of the present invention, a Myrothecium roridum is provided, and the preservation number of the Myrothecium roridum in China Center for Type Culture Collection (CCTCC) is CCTCC No. M205120.

Other aspects of the invention will be apparent to those skilled in the art from the disclosure herein.

Description of drawings

Figure 1. Colony photo of Myromyces dewwet strain HNSD2003120101 cultured on potato sucrose agar (PSA) for 15 days. The green dots are spore clusters.

Fig. 2. Microscopic observation photos of the morphology of Myrophyllum dewwet strain HNSD2003120101. Wherein, Fig. 2A is the conidia (Sporodochium); Fig. 2B is the head structure of the conidia; Fig. 2C is the branch of the conidiophores; Fig. 2D is the spore mass on the culture medium; Fig. 2E is the conidia spore.

Fig. 3. Symptoms after inoculation of water hyacinth with Myrophyta dewwet strain HNSD2003120101. The whole plant of water hyacinth was sprayed and inoculated with 10 ⁶ -10 ⁷ spores/ml spore liquid. Figure 3A shows the disease status of water hyacinth 7 days after inoculation, and Figure 3B shows a photo of the control water hyacinth grown in clear water.

Detailed ways

For a long time, the inventor has always been committed to the development of safe and effective water hyacinth control biological agents. After extensive research and screening, it was found that Mycobacterium dewwet is a microorganism capable of killing water hyacinth. Repeated tests have shown that Mycobacterium dewwet has good water hyacinth-specific pathogenicity, but does not have any pathogenicity to many major crops (including tomato, cucumber, etc.), so the strain has a high degree of pathogenicity when used in the field. security. The present invention has been accomplished based on this.

And, the inventor has also found a dew-wet paint spot bacteria strain (preservation number: CCTCC No.M205120) that can strongly and rapidly inhibit the growth of water hyacinth, and the inventor named the strain "dew-wet paint spot Bacteria HNSD2003120101".

Myromyces dew

Myrothecium roridum belongs to the half-knowledge phylum of the fungi kingdom. The conidia are produced from the host's epidermis or the spore under the cuticle or from the hyphae. The upper broom-like branches, with 3-7 bottle-shaped stalks growing in whorls on the top, the stalks are slender, club-shaped, straight and colorless, 8-10 μm long; conidia are born on the top, colorless at first, and then pale Green, dark green when piled up, buried in glue, oval to short rod-shaped, 3-4×1-1.4μm (5-9×1-2.5μm).

As used herein, Mycobacterium dewwet HNSD2003120101 refers to Mycobacterium dewwet with the preservation number of CCTCC No.M205120. Mycobacterium dewwet HNSD2003120101 colonies are white and flat on PDA (potato dextrose agar medium), and the diameter reaches 50-60mm in 10 days on PDA at 25°C; the surface produces small clusters of bulges, which are sub-seats, starting as White, and gradually turn green or dark green due to sporulation in the later stage; no pigment is produced on the back, see Figure 1. Hyphae 1.5-2.0 μm wide, separated; conidiophores not clearly differentiated, with dense bristle-like branches at the top; small peduncles columnar, whorls on the tops of spore branches. Generally, multiple spores grow in clusters to form conidia. Conidia are colorless to green, cylindrical, truncated at both ends, 5.0-6.6×1.5-1.9μm, see Figure 2. Conidiophores are generally formed on conidiophores. The bacterial strain can grow at 20°C-32°C, and the optimum temperature is 24-28°C.

Described bacterial strain is in cultivation, except that the back side of bacterium colony is colorless, and Domsch (1980) describes in its monograph (the back side has yellow or red pigment) in conidiophore morphology, conidia size, shape and form conidiophores The characteristics of spores are consistent. By sequencing the ribosome-DNA ITS region and searching for similar sequences on the Internet, it can be seen that the isolate sequence is in the ITS region (ribosomal DNA) with the two sequences submitted by Hagedorn, G.M (2000) (GenBank: AJ301995, AJ302001). internal transcriptional spacer) has 8 base differences, the similarity reaches 98%, and the difference sites are all in ITS1 and ITS2, which belong to the variable region. Therefore, the inventor identified that the isolate is the Mycobacterium dewwet ( Myrothecium roridum).

As long as the Mycobacterium dewwet of the present invention is obtained, and according to the characteristics of the Mycobacterium dewwet provided by the present invention, the method of mass-cultivating Mycobacterium dewwet and making it produce a large number of spores is familiar to those skilled in the art .

There are many kinds of culture medium suitable for cultivating Myrophyllum varrosum. It should be understood that any medium can be used as long as it can provide nutrient components necessary for the growth and reproduction of Myrophyllum var. In the prior art, the culture medium for cultivating other various Mycobacteria dewwet all is applicable to the cultivation of Mycobacteria dewwet, such as can adopt natural culture medium (such as potato sucrose, oat, wheat, barley, or media made of rice, etc.). The medium can be solid or liquid.

In a preferred mode of the present invention, the spore inoculum of Mycobacterium dewwet is carried out by the following method: natural medium such as solid medium potato sucrose, oat, wheat, barley or rice or corresponding liquid culture liquid (preferably solid medium), 20-32°C (preferably 24-28°C), in the dark or light (preferably dark), cultured for 10-30 days (preferably 15-20 days), can form a large number of spores .

Pesticide formulation

The invention provides a pesticide composition, which contains an effective amount of Myromyces dew varnish or its spores, and the rest of a pesticide acceptable carrier.

In the present invention, the term "comprising" means that various components can be used together in the mixture or composition of the present invention. Accordingly, the terms "consisting essentially of" and "consisting of" are included in the term "comprising".

In the present invention, "pesticide acceptable" ingredients are suitable for agricultural purposes without excessive adverse side effects (such as toxicity, irritation and allergy) on humans or other animals, plants other than water hyacinth, that is, there are A substance with a reasonable benefit/risk ratio.

In the present invention, "pesticide-acceptable carrier" is an acceptable solvent, suspending agent or excipient for delivering the Myrophyllum dew spp. HNSD2003120101 or its spores of the present invention to water hyacinth. The carrier can be liquid or solid, preferably a carrier capable of maintaining the activity of Myromyces varnish HNSD2003120101 or its spores to a relatively high degree.

The dosage form of the pesticide composition can be various, including but not limited to: wettable powder, emulsifiable concentrate, aqueous solution, emulsion, sprayable solution, oily or aqueous dispersion, suspension, powder, granule, or microcapsules. It should be understood that any dosage form is acceptable as long as it can deliver the Myromyces dew varnish HNSD2003120101 or its spores to water hyacinth plants under the premise of maintaining all or part of their activity. Those formulations that are easy to deliver are preferred, and in a preferred form of the invention, the pesticide composition is a liquid spray, or spray.

In the present invention, the adjuvant is an auxiliary component that plays an auxiliary adjustment function in the composition, for example, it may be a surfactant, an adhesion aid or other types of adjuvants.

The content of the active ingredient in the concentrated pesticide composition is relatively high, such as 20-90%, while the content of the active ingredient in the diluted pesticide and the actual composition is relatively low, usually 0.00005-0.5%. In addition, other suitable chemical agents, synergists, trace elements, stabilizers, binders, wetting agents, dispersants, emulsifiers, penetrants, solvents, fillers and other common components may also be included. The pesticide composition of the present invention may also contain other active herbicides.

The pesticide composition of the present invention may contain 10 ⁴ -10 ¹² Mycobacterium dewwet or its spores/gram (mL) of the composition; more preferably, it contains 10 ⁶ -10 ¹⁰ Mycobacterium dewwet or its spores per gram (mL) of composition.

In preparing the compositions, suitable solid diluents include, but are not limited to, diatomaceous earth, corn hulls, tricalcium phosphate, cork flour, clays such as kaolin, bentonite, or attapulgite, and water-soluble polymers.

In addition, solid compositions may contain one or more compatible wetting agents, dispersants, emulsifiers or pigments, which may also act as diluents in the solid state.

Such solid compositions may be in the form of powders, granules or wettable powders. Dusts are usually obtained by grinding, granules, tablets or bricks by granulation or tableting.

Liquid compositions may take the form of solutions, suspensions and emulsions, may be encapsulated in natural or synthetic polymers, and may contain wetting, dispersing or emulsifying agents. Such emulsions, suspensions or solutions may be prepared with aqueous, organic or aqueous-organic diluents for the water-soluble polymers (and mixtures of such diluents). In addition, the diluent may contain, for example, the above-mentioned ionic or non-ionic wetting agent, dispersant or emulsifier or a mixture thereof.

The principles of various formulations are known and described, for example, in Winnacker-Kuchler, "Chemische Technologie ", Vol. 7, C. Hauser Verlag Munich, 4th Edition, 1986; van Valkenburg, "Pesticide Formulations", Marcel Dekker NY, 2nd Edition, 1972-73; K. Martens, "Spray Drying Handbook", 3rd Edition, G. Goodwin Ltd. London.

The required formulation adjuvants, (such as inert substances, surfactants, solvents and other additives) for the compositions of the present invention are also known and described for example in: Watkins "Handbook of Pesticidal Powder Diluents and Carriers" pp. 2nd ed., Darland Books, Caldwell NJ; Hv Olphen, "A Guide to the Chemistry of Clay Colloids" 2nd ed., J. Wiley & Sons, NY, Marsden, "A Guide to Solvents" 2nd ed., Interscience, NY 1950; McCutcheon's, "Descaling Agents and Emulsifiers Annals", MC Publ.Corp., RidgewoodN.J.; Sisley and Wood, "Encyclopedia of Surfactants", Chem.Publ.Co.Inc., NY1964; Schonfelt, "Grenzflachenaktive Athylenoxidaddukte" Surface Active Epoxy Ethane Addition Products , Wiss. Verlagsgesell., Stuttgart 1976; Winnacker-Kuchler, "Chemische Technologie ", Vol. 7, C. Hauser Verlag Munich, 4th edition, 1986.

Wettable powder can be uniformly dispersed in water. In addition to active substances, wettable powders can also contain wetting agents, dispersants, diluents and other non-environmentally harmful substances. Dusts may be prepared by grinding the active substance with finely divided solid matter such as talc, kaolin, natural clay such as bentonite, or diatomaceous earth. Granules can be prepared by spraying the active substance adsorbed on the particles of the inert substance, or by applying a solution of the active substance to a carrier (such as sand, kaolin or inert substance) through a binder (such as polyvinyl alcohol, sodium polyacrylate, or mineral oil). particles) on the surface. If it is intended to be applied in admixture with chemical fertilizers, suitable active substances can be prepared as granules in the same way as fertilizer granules.

In a preferred mode of the present invention, the pesticide composition is in the form of a liquid (sprayable solution), containing 10 ⁴ -10 ¹² spores of Mycobacterium dewwet spores per mL, and the balance Acceptable carriers or adjuvants; preferably, the pesticide composition may contain 10 ⁵ -10 ⁹ spores/mL of Mycobacterium versicolor spores, and the balance of pesticide acceptable carriers or adjuvants; more Preferably, the pesticide composition may contain 10 ⁶ -10 ⁸ spores/mL of M. dew spores, and the balance of pesticide acceptable carriers or adjuvants. Moreover, the pesticide composition also contains 0.001-0.01% (V/V) surfactant (such as Tween 20 or NP-40).

Methods of inhibiting the growth of water hyacinth

The present invention also provides a method for inhibiting the growth of water hyacinth, the method comprising: applying an effective amount of the var. dew varnish or its spores of the present invention to the water hyacinth.

When the dew-wet paint spot fungus or its spores of the present invention are applied to water hyacinth, the effective application amount is generally 10 ⁵ -10 ¹² /m ² water hyacinth; more preferably, the dew-wet paint spot of the present invention The application rate of bacteria or spores is 10 ⁶ -10 ¹⁰ /m ² water hyacinth.

In a preferred example of the present invention, the spores of the varnish varnish are prepared in the form of bacterial solution, and applied to the leaves of water hyacinth by spraying.

In a preferred mode of the present invention, the described Mycobacterium dewwet or its spores are used to control water hyacinth by the following method: Inoculate Mycobacterium dewwet on a suitable medium, cultivate at a suitable temperature, and form a large number of After the spores of the spores of the lacquer varnish, wash the spores of the spores of the spores of the varnish with a certain volume of tap water to make a bacterial solution, filter through gauze or small-pore nylon cloth and other materials, and dilute to 10 ⁶ -10 ⁸ spores/ml spore liquid (preferably ¹⁰⁷ spores/ml), add a certain concentration of Tween 20 or NP-40 or other surfactants, spray the positive and negative sides of water hyacinth inoculation, after 2-3 days on the water hyacinth A large number of lesions begin to appear, and a large number of large lesions and necrotic leaves can appear after 5-7 days.

Main advantage of the present invention:

(1) For the first time, a strain that is particularly effective in inhibiting the malignant aquatic plant-water hyacinth has been found, which provides an extremely effective way for biological control of water hyacinth.

(2) The pesticide composition prepared by the invention can specifically inhibit the growth of water hyacinth without visible toxicity to other animals or plants, and is a safe and environment-friendly product.

Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. For the experimental methods without specific conditions indicated in the following examples, the conventional conditions or the conditions suggested by the manufacturer are usually followed.

Unless otherwise defined, all professional and scientific terms used herein have the same meanings as commonly understood by those skilled in the art. In addition, any methods and materials similar or equivalent to those described can also be applied in the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only.

Example 1 Acquisition of Mycobacterium dewwet strain HNSD2003120101

The present inventor has isolated dozens of microbial strains from water hyacinth in Shanghai and some other areas in China, and determined their pathogenicity to water hyacinth under controlled and natural conditions. After long-term experiments, a strain of Myrothecium roridum (No.: HNSD2003120101) was found, which has a strong and rapid pathogenic effect on water hyacinth. At the same time, the inventors have tested many major crops (including tomato, cucumber, etc.) with the strain, without any pathogenic effect, indicating that the strain has high safety when used in the field.

Example 2 The preparation method of the conidia inoculum of Mycobacterium dewwet varnish HNSD2003120101

In the present embodiment, two kinds of methods for preparing the conidia inoculum of Myromyces versicolor are provided, specifically as follows:

method one

Inoculate the PSA (potato sucrose agar medium) plate center of the Mycobacterium dewwet bacteria block, and culture it under 25°C and dark conditions for 15 days, and many dark green dots, namely conidia, appear on the bacterial colony. The spores were stained with the seed ring, streaked with PSA, inverted, and cultured for 15-20 days at 25°C in the dark, and a large number of conidia could be formed on the plate (Figure 1).

Method Two

Put 120 grams of wheat grains or rice into a 500 ml triangular flask, add water according to the ratio of adding 10 ml of water to every 10 grams of wheat or rice grains, sterilize with high-pressure steam at 121°C for 30 minutes, and set aside. Inoculate the Mycobacterium dewwet bacteria block to the center of the PSA plate, and culture it under dark conditions at 25°C for 15 days. Many dark green dots, namely conidia, appear on the bacterial colony. Cut the colony into 2-3mm small bacterial blocks, add 20-30 small bacterial blocks to each bottle of wheat or rice medium, mix well and culture in the dark at 25°C, shake once a day, after 15-20 days of cultivation, the wheat or grain A large number of green conidia will form on the grain.

Example 3 Determination of the 18S rRNA fragment, ITS1, 5.8S and ITS2 of ITS1, 5.8S and ITS2

The total DNA of the Mycobacterium dewwet strain HNSD2003120101 of the present invention was extracted by conventional methods, and the ITS of the fungus was amplified with universal primers ITS5 and ITS4 (ITS5: 5'-ggaagtaaaagtcgtaacaagg-3'; ITS4: 5'-tcctccgcttattgatatgc-3') Section, the measured sequence is as follows (SEQID NO: 1): AACAAGGTCT CCGTTGGTGA ACCAGCGGAG GGATCATTAC CGAGTTTACA AACTCCCAAA 60

CCCTTTGTGA ACCTTACCTA TCGTTGCTTC GGCGGGACCG CCCCGGCGCC TTCGGGCAAC 120

GGAACCAGGC GCCCGCCGGA GAACCCAAAC TCTTATGTCT TTAGTGGTTT TCTCCTCTGA 180

GTGACACATA AACAAATAAA TAAAAACTTT CAACAACGGA TCTCTTGGTT CTGGCATCGA 240

TGAAGAACGC AGCGAAATGC GATAAGTAAT GTGAATTGCA GAATTCAGTG AATCATCGAA 300

TCTTTGAACG CACATTGCGC CCGCCAGTAT TCTGGCGGGC ATGCCTGTTC GAGCGTCATT 360

TCAACCCTCA GGCCCCCAGT GCCTGGCGTT GGGGATCGGC GTGGGCCGGG GCGTCCTCCG 420

GGACGTCCCC GCGCCTGCCG GCCCCGAAAT TCAGTGGCGG TCTCGCTGTA GTCCCCCTCT 480

GCGTAGTAGC ACAACTCGCA TTGGAGCTCG GCGGTGGCCA TGCCGTAAAA CACCCCACTT 540

CTGAAAGTTG ACCTCGGATT 560

Example 4 BLAST Analysis of ITS Segment DNA Sequence of Myromyces dew varnish HNSD2003120101

The sequence of the ribosome-DNA ITS region determined above was compared with various known sequences using the BLAST program (the website of the American Center for Biological Information, NCBI, www.ncbi.nlm.nih.gov).

It can be seen by sequencing the ribosome-DNA ITS region and searching for similar sequences on the Internet that the sequence of this isolate and the two sequences submitted by Hagedorn, G.M (2000) (GenBank: AJ301995, AJ302001) have 8 bases in the ITS region. The difference, the similarity reaches 98%, and the difference sites are all in ITS1 (40-202 of SEQ ID NO: 1) and ITS2 (361-546 of SEQ ID NO: 1), which belong to the variable region.

The pathogenic effect of varnish spot fungus on water hyacinth under natural conditions in embodiment 5

The above method forms a large number of spores of varnish varnish on the culture medium, washes it with a certain volume of tap water to make a bacterial solution, filters it through gauze or nylon cloth with small aperture and other materials, and dilutes it into 10 ⁶ -10 ⁸ spores /ml spore liquid (preferably ¹⁰⁷ spores/ml), add 0.01% (V/V) Tween 20 or NP-40 or other tensio-active agent, spray inoculate 500mL bacterium liquid by every square meter water hyacinth, spray inoculation The front and back of water hyacinth.

As a result, it was found that under completely natural conditions, many brown lesions with a size of several millimeters were produced on the blade, petiole and part of the water hyacinth after spraying for 2-3 days. After 1 week of spraying, the damage to the leaves of the plants increased, and the scabs healed into sheets. The dead area of many leaves was more than 50%, and the disease index (referring to the statistical results of the incidence and severity within the scope of investigation) exceeded 40%, as shown in Figure 3A. Compared with the control Figure 3B, a large number of leaves of water hyacinth in Figure 3A withered.

Example 6 Safety test of Mycobacterium dewwet on plants other than water hyacinth

The above method forms a large number of spores of varnish varnish on the culture medium, washes it with a certain volume of tap water to make a bacterial solution, filters it through gauze or nylon cloth with small aperture and other materials, and dilutes it into 10 ⁶ -10 ⁸ spores / milliliter spore liquid (preferred ¹⁰⁷ spores/ml), add 0.01% (V/V) Tween 20 or NP-40 or other tensio-active agent, the spore liquid spray inoculation of pathogen to 3-4 true leaves Cucumber, tomato, eggplant, cabbage, cylinder bean, pumpkin, corn, green vegetables, and muskmelon plants were sprayed with water as a control. After the spore liquid on the plants was just dry, they were moved to a light incubator for 12 hours of light at 25°C. Grow under moisturizing conditions, and observe the disease of the plants regularly.

All crops were inoculated one week, two weeks and all growth stages thereafter without any disease spots, and the control crop plants sprayed with clear water did not see any disease spots either.

Culture preservation

Myrothecium roridum HNSD2003120101 of the present invention has been preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC No.M205120, and the preservation date is October 19, 2005.

All documents mentioned in this application are incorporated by reference in this application as if each were individually incorporated by reference. In addition, it should be understood that after reading the above teaching content of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

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Claims (10)

1. the purposes of a kind of lacquer spot fungus or its spore, it is characterized in that, be used for preparing the preparation that suppresses the growth of water hyacinth. 2. purposes as claimed in claim 1, is characterized in that, described varnish spot fungus is the varnish spot bacterium whose preservation number is CCTCC No.M205120. 3. A pesticide composition, characterized in that it contains an effective amount of Myromyces dew varnish or its spores, and a remaining amount of a pesticide acceptable carrier. 4. The pesticide composition according to claim 3, wherein it is in a dosage form selected from the group consisting of wettable powder, emulsifiable concentrate, aqueous solution, emulsion, sprayable solution, oily or aqueous dispersion, suspension formulations, powders, granules, or microcapsules. 5. The pesticide composition according to claim 4, wherein the pesticide composition is in the form of a sprayable solution. 6 . The pesticide composition according to claim 3 , characterized in that it contains 10 ⁴ -10 ¹² Myromyces dew spp. or its spores per gram (or milliliter) of the composition. 7. The pesticide composition according to claim 3, characterized in that, the composition further contains substances selected from the group consisting of surfactants, spreading agents, filling substances, or germ activity accelerators. 8. The pesticide composition as claimed in claim 5, characterized in that: containing Mycobacterium dew varnish or its spores: 10 ⁴ -10 ¹² /mL; Surfactant: 0.001-0.01% (V/V); and The balance is water. 9. A method for inhibiting the growth of water hyacinth, characterized in that the method comprises: applying an effective amount of Mycobacterium dewwet and its spores on water hyacinth. 10. A varnish varnish, characterized in that the preservation number of the varnish rot in the China Center for Type Culture Collection is CCTCC No. M205120.

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