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Device and method for detecting jam of sample needle of biochemical analyser

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Publication number
CN101004424B
CN101004424B CN 200610033283 CN200610033283A CN101004424B CN 101004424 B CN101004424 B CN 101004424B CN 200610033283 CN200610033283 CN 200610033283 CN 200610033283 A CN200610033283 A CN 200610033283A CN 101004424 B CN101004424 B CN 101004424B
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needle
circuit
variable
specimen
detection
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CN 200610033283
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Chinese (zh)
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CN101004424A (en )
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侯金龙
朱星才
李伟
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深圳迈瑞生物医疗电子股份有限公司
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Abstract

A device used for detecting out blocked specimen needle of biochemical analyzer is prepared for setting specimen needle to by variable impedance and variable capacity type, arranging liquid level detection circuit on detection circuit, switching specimen needle as variable impedance into liquid level detection circuit, detecting impedance variation of said variable impedance by liquid level detection circuit and outputting detected signal. The method utilizing said device to detect blocked specimen needle on biochemical analyzer is also disclosed.

Description

生化分析仪样本针堵塞检测装置及方法【技术领域】[0001] 本发明涉及生化分析仪样本针堵塞检测装置及方法。 Biochemical analyzer sample needle clogging detection device and method TECHNICAL FIELD [0001] The present invention relates to a biochemical analyzer sample needle clogging detection device and method. 【背景技术】[0002] 对于全自动生化分析仪来说,液面检测针特别是样本针,很可能因为吸入纤维蛋白或血细胞而堵塞,造成加样不准确,甚至无法正常加样,在操作者不知情的情况下这将严重影响测量结果,所以很有必要对样本针的堵塞情况进行判断。 BACKGROUND OF THE INVENTION [0002] For automatic biochemical analyzer, a liquid level detecting sample needle needle particular, probably because the suction fibrin or blood cells clogging, resulting in inaccurate loading or even not properly loaded, in operation blinded case which will seriously affect the measurement result, it is necessary to sample needle clogging is determined. [0003] 为此,在专利US6,663,353中描述了一种样本针堵塞检测系统,包括两个检测传感部分,一部分实现液面检测,另一部分通过压力传感器来检测液路通道内压力的变化从而判断液面检测针的状况。 [0003] For this reason, in patent US6,663,353 describes a sample needle clogging detection system comprising two detection sensor part, part of the implementation level detection, another portion of the fluid passage detected by the pressure sensor pressure passage thereby determining the state of change in liquid level detection needle. 当样本针接触液面准备吸样时,液路通道内部压力状况会突然变化,从而产生了一个明显的跳变,在吸样结束之后也将产生一个内部压力的突然变化,压力值恢复,这样,就形成了一个压力脉冲,通过对压力脉冲信号进行复杂的运算,并与经验值比较来判断吸液是否正常。 When the liquid surface in contact with the sample preparation aspirating needle, the internal pressure of the liquid channels conditions suddenly change, resulting in a significant jump, after the aspirating will also produce a sudden change in the internal pressure, the pressure value recovery, such , a pressure pulse is formed, the pressure pulse signal by complex calculations and comparison with empirical values ​​to determine whether the normal pipetting. 因为正常信号与非正常信号脉宽不一样,所以可以由此来判断不正常的工作状态,如样本针堵塞等。 Because the normal signal and the abnormal signal is not the same width, whereby it is possible to determine abnormal operating state, such as sample needle clogging. [0004] 上述用压力检测样本针是否堵塞的方法是目前解决样本针堵塞判断的有效途径, 而且压力检测也能同时实现液面检测功能。 [0004] The method of the above-described pressure detecting sample needle clogging is efficient alternative sample needle clogging determination, but also can simultaneously achieve pressure detection level detection function. [0005] 但是压力检测液面的方法易受干扰,因此,为了既能可靠地实现液面检测功能,又能实现液面检测针的堵塞判断,往往需要两种检测方法配合使用,结构较为复杂。 [0005] However, the pressure sensing methods susceptible to interference level, therefore, in order to reliably achieve both the liquid level detection function, but also to achieve the clogging determination level detection needles often require two detection methods with the use of a more complex structure . 【发明内容】[0006] 本发明的目的在于提供一种生化分析仪样本针堵塞检测装置及方法,能够简便可靠地实现堵塞检测。 SUMMARY OF THE INVENTION [0006] The object of the present invention is to provide a biochemical analyzer sample needle blockage detection apparatus and method, the clogging detection can be achieved easily and reliably. [0007] 为实现上述目的,本发明提出的生化分析仪样本针堵塞检测装置包括样本针、检测电路,其特征是:所述样本针为可变阻抗;所述检测电路包括液面检测电路;所述样本针作为可变阻抗接入到液面检测电路中,液面检测电路检测所述可变阻抗的阻抗变化,并将检测信号输出。 [0007] To achieve the above object, biochemical analyzer sample needle made according to the invention comprises a sample needle clogging detecting means, a detection circuit, wherein: the sample needle is variable impedance; the level detection circuit comprises a detection circuit; the sample needle access to the level detection circuit, the level detection circuit detects the change in impedance of the variable impedance, outputs a detection signal as a variable impedance. [0008] 优选地,本装置所述可变阻抗为可变电容。 [0008] Preferably, the said variable impedance means is a variable capacitance. [0009] 优选地,本装置所述可变电容为双层同轴结构,内层与外层之间用绝缘材料隔绝, 内层和外层分别作为可变电容的两个极板。 [0009] Preferably, the said variable capacitance means is isolated with an insulating material between the double-layer coaxial structure, inner and outer layers, inner and outer, respectively, as two plates of a variable capacitor. [0010] 优选地,本装置所述液面检测电路包括振荡源、仪表放大器和平衡电路,所述振荡源所产生的信号一路经平衡电路的第一支路输入到仪表放大器的负输入端,另一路经平衡电路的第二支路输入到仪表放大器的正输入端,样本针内外两层分别作为可变电容的两个极接入到平衡电路的第二支路中;所述平衡电路设定为当可变电容的电容值为特定值时, 其输入到仪表放大器的正输入端和负输入端的信号保持平衡,当可变电容的电容值偏离特定值时,其输入到仪表放大器的正输入端和负输入端的信号产生差异。 [0010] Preferably, the apparatus of the level detection circuit includes an oscillation source, and balancing instrumentation amplifier circuit, the signal generated by the oscillation source one input to the negative input of the instrumentation amplifier via a first branch of the balancing circuit, balancing circuit further path input to the second branch of the positive input terminal of the instrumentation amplifier, the sample needle as outer and inner layers, respectively, two electrode access to a variable capacitance balancing circuit in the second branch; the balance circuit provided when the value is set to a particular value of the variable capacitance capacitor, which is input to the positive input of the instrumentation amplifier and the negative input terminal of the balanced signal, when the capacitance value of the variable capacitance deviates from a specific value, which is the positive input to instrumentation amplifier a signal input terminal and a negative input terminal of a difference. [0011] 优选地,本装置还包括控制板、上位机,所述控制板一端与检测电路相连,另一端与上位机相连;所述检测电路中还包括有依次相连的A/D变换电路、CPU、接口电路,所述A/ D变换电路的输入端与液面检测电路相连,所述接口电路的输出端与控制板的输入端相连。 [0011] Preferably, the apparatus further comprises a control panel, the host computer, the control panel is connected to one end of the detection circuit, and the other end is connected with the host computer; said detection circuit further includes sequentially connected to an A / D conversion circuit, input terminal of the CPU, the interface circuit, said a / D converting circuit and the level detection circuit connected to an output terminal connected to the input of the interface circuit and the control board. [0012] 优选地,本装置所述检测电路中还包括有温度传感电路,其输出端与A/D变换电路的另一输入端相连。 [0012] Preferably, the apparatus further comprising a detection circuit with a temperature sensing circuit, the other input of which is connected to the output terminal of the A / D conversion circuit. [0013] 本发明还包括一种生化分析仪样本针堵塞检测方法,其特征是包括如下步骤:A、 将样本针作为可变阻抗接入到液面检测电路中;B、利用检测电路检测所述可变阻抗的阻抗变化,并将检测信号输出;C、根据输出的信号判断样本针是否堵塞。 [0013] The present invention further comprises a biochemical analyzer sample needle clogging detecting method, characterized by comprising the steps of: A, the sample needle access to the variable impedance as the liquid level detection circuit; B, using the detection circuit detects the change in the impedance of said variable impedance, and outputs a detection signal; C, based on a signal output from the sample needle is blocked. [0014] 优选地,本方法所述可变阻抗为可变电容。 [0014] Preferably, the method of the variable impedance is a variable capacitance. [0015] 优选地,本方法步骤B中检测电路检测所述可变阻抗的阻抗变化的方法包括如下步骤:B1、利用振荡源所产生信号;B2、将所述信号一路经平衡电路的第一支路输入到仪表放大器的负输入端,另一路经平衡电路的第二支路输入到仪表放大器的正输入端;B3、仪表放大器将检测到的信号输出;其中,样本针内外两层分别作为可变电容的两个极接入到平衡电路的第二支路中;所述平衡电路设定为当可变电容的电容值为特定值时,其输入到仪表放大器的正输入端和负输入端的信号保持平衡,当可变电容的电容值偏离特定值时,其输入到仪表放大器的正输入端和负输入端的信号产生差异。 [0015] Preferably, the change in impedance of the variable impedance method according to method step B, said detection circuit detecting comprises the steps of: B1, by using the oscillation signal generated by the source; B2, the balanced signal all the way through the first circuit branch input to the negative input of the instrumentation amplifier, a second balancing circuit path other branch input to the positive input terminal of amp; B3, instrumentation amplifier to the detection output signal; wherein the outer and inner layers, respectively, as sample needle the two variable capacitance electrode access to the second branch balancing circuit; said balancing circuit is set when a specific capacitance value of variable capacitance value, which is input to the positive input terminal of the instrumentation amplifier and a negative input balanced signal terminal, when the capacitance value of the variable capacitance deviates from a specific value, which is input to the positive input of the instrumentation amplifier and the negative input terminal of a difference signal. [0016] 优选地,本方法在步骤B之后还包括如下步骤:将输出的检测信号依次输入到A/D 变换电路、CPU、接口电路、控制板、上位机。 [0016] Preferably, the method further comprises, after step B, the step of: sequentially outputs the detection signal is input to the A / D conversion circuit, CPU, an interface circuit, control board, PC. [0017] 优选地,本方法在步骤B中还检测环境温度,并将环境温度检测值依次输入到A/D 变换电路、CPU、接口电路、控制板、上位机。 [0017] Preferably, in the method step B it is also detected ambient temperature, and the ambient temperature detection value sequentially input to the A / D conversion circuit, CPU, an interface circuit, control board, PC. [0018] 优选地,本方法将因堵塞造成的工作电压变化量按如下公式校正:[0020] 其中Vt为可变阻抗与温度的变化率,Tl为初始状态环境温度,Va为初始状态时液面检测电路工作电压,Vb为可变阻抗改变后的液面检测电路工作电压,T2为通过温度传感电路信号得到的Vb时的环境温度;当工作电压变化量大于设定的阈值则判断样本针堵塞。 [0018] Preferably, the present method the amount of voltage change caused by the correction work due to clogging by the following formula: [0020] where Vt is a variable rate of change of impedance and temperature, Tl is an ambient temperature the initial state, the initial state is Va of liquid surface detection circuit voltage, Vb is the voltage level detection circuit of a variable impedance change, T2 to ambient temperature at the temperature sensing circuit signal Vb obtained; when the operating voltage threshold variation is greater than the sample set is determined needle blockage. [0021] 与现有技术相比,本发明具有如下优点:[0022] 由于本发明将液面检测针作为一个广义的阻抗(优选为电容)接入到检测电路中,检测电路能够通过工作电压来计算当前可变电容(针电容)的大小,当液面检测针接触液面时其可变电容(针电容)会有较大的变化,同时,工作电压也会相应变化,并根据可变电容(针电容)的变化来判断液面检测针是否接触到了液面,因此,本发明能够利用电容液面检测方法能够可靠地实现液面检测功能,同时,不再需要对液路系统进行任何修改,不需要压力传感器及处理电路。 [0021] Compared with the prior art, the present invention has the following advantages: [0022] Since the present invention detects the level of the needle as a generalized impedance (preferably capacitive) access to the detection circuit, the detection circuit through the operating voltage calculates the current size of the variable capacitor (capacitor needle) of the contact pin when the liquid surface level detecting variable capacitance thereof (pin capacitance) vary greatly, at the same time, the operating voltage will change accordingly, and the variable capacitance (capacitance needle) to determine the change in the level detection pin is in contact to the liquid level, therefore, the present invention is capable of using capacitive liquid level detecting method capable of reliably liquid level detection, and eliminates the need for any fluid path system modification, does not require pressure sensors and a processing circuit. 当液面检测针被样本中的纤维蛋白或血细胞等堵塞了,其可变电容(针电容)也会明显变化,配合当前环境温度值并能够判断液面检测针是否堵塞。 When the needle is blocked liquid level detecting sample fibrin or blood cells, which is a variable capacitance (capacitance pin) will significantly change with the value of the current ambient temperature can be determined that the liquid level detection and the needle is blocked. 因此,本发明利用电容液面检测的方法可靠地检测液面的同时,利用液面检测针本身电容的变化规律,能够简便可靠地实现堵塞检测。 Accordingly, while the present invention is a method of using a capacitive level detection reliably detects the liquid surface, using a variation of the capacitance liquid level detecting needle itself, occlusion detection can be achieved easily and reliably. [0023] 结合简单的温度传感电路,可使检测结果更加准确。 [0023] combined with simple temperature sensing circuit enables more accurate detection result. 【附图说明】[0024] 图1是本发明实施例生化分析仪样本针结构图;[0025] 图2是本发明实施例生化分析仪样本针被堵塞示意图;[0026] 图3是本发明实施例液面检测系统示意图;[0027] 图4是本发明实施例液面检测电路示意图;[0028] 图5是本发明实施例液面检测可变电容(针电容)随环境温度变化曲线。 BRIEF DESCRIPTION [0024] FIG. 1 is a biochemical analyzer sample needle configuration diagram of embodiment of the present invention; [0025] FIG. 2 is an embodiment of the present invention biochemical analyzer sample needle is blocked schematic; [0026] FIG. 3 of the present invention is level detection system schematic diagram of an embodiment; [0027] FIG. 4 is a schematic diagram of the liquid level detection circuit of the present embodiment of the invention; [0028] FIG. 5 is a variable capacitance liquid level detector (pin capacitance) embodiment of the present invention, with the ambient temperature curve. 【具体实施方式】[0029] 实施例一[0030] 本方案是关于检测样本针堵塞的方法,利用了样本针堵塞后可变电容(针电容) 有较大变化的规律、可变电容(针电容)随环境温度变化的规律,以及可变电容(针电容) 与工作电压的一一对应关系来实现样本针的堵塞检测。 DETAILED DESCRIPTION Example embodiments] [0029] a [0030] The present embodiment relates to a method of detecting clogging sample needle, using the law of large variations in the variable capacitor (capacitor needle) after the sample needle clogging variable capacitance (needle capacitance with temperature changes in the law), and one correspondence between a variable capacitance (capacitance pin) to achieve the operating voltage test sample needle clogging. 其中,我们采用了温度传感电路来指示当前温度,以便能够消除环境温度造成的对可变电容(针电容)的影响。 Wherein we used to indicate the current temperature of the temperature sensing circuit, to be able to eliminate the influence of ambient temperature caused by variable capacitance (pin capacitance). [0031] 先看样本针4的结构,如图1所示,样本针4为双层同轴结构,内层如与外层4b 之间用绝缘材料4c隔绝,样本针4作为一个广义的可变电容接入到液面检测电路5中,内层如和外层4b分别作为可变电容(针电容)的两个非规则的极板。 [0031] Look at the sample needle 4 structure, shown in Figure 1, the sample 4 is double needle coaxial structure, such as an inner layer between the outer layer 4b and 4c isolated with an insulating material, as a broad sample needle 4 may variable capacitance access to the liquid level detecting circuit 5, an inner layer and an outer layer 4b, respectively, as a variable capacitor plate (capacitance pin) of two irregular. [0032] 根据电容器原理,电容器的电容量可以由电极面积S [m2],两极板间距离t[m]以及电介质的相对介电常数ε来表示,即[0033] C(¥) ^ ε* (1)[0034]其中,ε 0 = 8. 85*10_12F/M,为一个常量。 [0032] According to the principle of a capacitor, the capacitance of the capacitor may be an electrode area S [m2], the distance between the plates t [m] and a relative dielectric constant [epsilon] is electrically represented medium, i.e., [0033] C (¥) ^ ε * (1) [0034] wherein, ε 0 = 8. 85 * 10_12F / M, is a constant. [0035] 因为样本针4结构已经固定,公式(1)中的ε与t并已确定,所以样本针4的电容量由电极面积S决定。 [0035] Since the structure of the sample needle 4 has been secured, the formula (1) and has been determined to t ε, so the sample needle 4 is determined by the capacitance of the electrode area S. 因空气的相对介电常数ε约为1,纯水的相对介电常数ε约为82,当样本针4接触样本杯10样本11液面后,可变电容(针电容)的一个极板边界发生了突变,相应地可以认为电极面积S发生了突变,因此样本针4接触液面后其可变电容(针电容)会有突然增大。 Due to the relative permittivity ε air is about 1, the relative dielectric constant ε of pure water is approximately 82, when the needle 4 is in contact with the sample 10 sample 11 sample cup level, variable capacitor (capacitor pin) is a boundary plate are mutated, it can be considered accordingly mutated the electrode area S, and therefore the pin 4 contacts the sample liquid surface which variable capacitance (capacitance pin) have suddenly increases. [0036] 样本针4吸取样本杯10中的样本11,可能造成样本针4堵塞,如图2所示,这种情况下,即使样本针4离开样本11液面处在空气中,因样本针4内层如的填充物发生改变, 使得可变电容(针电容)的一个电极的边界条件发生了改变,等同于电极面积S发生了改变,所以可变电容(针电容)同样会增大。 [0036] 4 sample needle 11 to draw the sample in the sample cup 10, the sample may cause clogging of the needle 4, 2, in this case, even if the sample away from the needle 4 at the level of the air sample 11, the sample needle because the inner layer of filler 4 is changed, so that the variable capacitance (capacitance needle) one electrode of the changed boundary conditions, equivalent to the electrode area S is changed, the variable capacitor (capacitor pin) will also increase. [0037] 下面详细描述检测样本针堵塞的装置与方法。 [0037] The apparatus and method for detecting clogging of the sample needle described in detail below. [0038] 要检测样本针堵塞,必须要一个液面检测系统如图3所示,整个系统包括样本针4、检测电路1、控制板2、上位机3(如PC)等。 [0038] To test sample needle clogging must be a level detection system shown in Figure 3, the system includes a sample needle 4, the detection circuit 1, the control panel 2, PC 3 (such as PC) and the like. 检测电路1是功能实现的核心,它包括液面检测电路5、温度传感电路6、A/D变换7、CPU 8、接口电路9等。 Detection circuit is a core function implemented, including the level detecting circuit 5, the temperature sensing circuit 6, A / D conversion 7, CPU 8, interface circuit 9 and the like. [0039] 液面检测电路5能够把样本可变电容(针电容)大小转化为当前工作电压,即工作电压与样本可变电容(针电容)是一一对应的,液面检测电路5的一种方案如图4所示, 信号源Vin分为两路分别输入到仪表放大器的两个输入端,Rl、Cl与R2、C2及样本可变电容(针电容)Cp组成平衡电路,信号Vin经Rl与Cl (平衡电路的第一支路)输入到仪表放大器的负输入端V-,信号Vin经R2、C2与可变电容(针电容)Cp (平衡电路的第二支路)输入到仪表放大器的正输入端V+,样本针4内外两层分别作为可变电容(针电容)Cp的两个极接入到电路中,其中外层4b接地,R3作为仪表放大器的增益调节电阻。 [0039] The level detecting circuit 5 to the sample can be a variable capacitance (capacitance-pin) is converted to the current size of the operating voltage, i.e. operating voltage variable capacitor sample (pin capacitance) is one to one, a level detection circuit 5 4 kinds of programs, signal source Vin is divided into two two input terminals respectively input to instrumentation amplifier, Rl, Cl and R2, C2 and the variable capacitance sample (needle capacitor) Cp compositional balance circuit, a signal Vin via Rl and CI (the first leg of the balanced circuit) is input to the negative input terminal of the instrumentation amplifier V-, signal Vin by R2, C2 and the variable capacitance (capacitance needle) Cp (the second branch of the balanced circuit) input to the meter amplifier positive input terminal V +, respectively, inner and outer sample needle 4 as two variable capacitors (capacitance needle) Cp access to the source circuit, wherein the ground layer 4b, R3 as the instrumentation amplifier gain adjusting resistor. 图4中其他标号为:51为振荡源,52为仪表放大器,53为电平变换电路。 Other reference numerals as in FIG. 4: oscillation source 51, instrumentation amplifier 52, a level conversion circuit 53. 所述平衡电路设定为当可变电容Cp的电容值为特定值时,其输入到仪表放大器52的正输入端V+和负输入端V-的信号保持平衡,当可变电容Cp的电容值偏离特定值时,其输入到仪表放大器52的正输入端V+和负输入端V-的信号产生差异。 The balancing circuit is set to a specific value when the capacitance of the variable capacitance Cp, which signal the positive input terminal of amp 52 V + and a negative input terminal V- is input to the balance, when the variable capacitance value of the capacitor Cp deviates from a specific value, the instrumentation amplifier 52 which is input to the positive input terminal V + and the negative input terminal V- of the signal variance. [0040] 当样本针接触液面使得可变电容(针电容)发生突变,引起仪表放大器输入端信号变化,从而使得输出信号Vo也发生突变,并通过A/D采样把模拟信号转换成数字信号,送入到CPU 8,经过信号处理后,通过接口电路9把液面检测信号13输出给控制板2。 [0040] When the sample liquid surface in contact with the needle such that the variable capacitance (capacitance needle) mutations, causing a change instrumentation amplifier input signal, so that the output signal Vo mutations occur, and through A / D sampling the analog signal into a digital signal , is fed to the CPU 8, after signal processing, via the interface circuit 13 outputs to the strap 2 9 level detection signal. [0041] 图3中,温度传感电路6能够把当前的环境温度转换成相应电压值,也送入到CPU 8,通过CPU 8软件计算就能够得到当前的环境温度值。 In [0041] FIG. 3, the temperature sensing circuit 6 to the current ambient temperature can be converted into a corresponding voltage value, also fed to the CPU 8, is calculated by the CPU 8 can be obtained the current software environment temperature. [0042] 在常温如25°C下,样本针清洗干净后处于某一个初始位置,未与液面接触,如生化分析仪清洗池上方,调整液面检测电路5,使得工作电压在一个合适的电平,设为Va,并能够计算对应的样本针4电容值。 [0042] at room temperature, such as at 25 ° C, the sample needle is clean after a certain initial position not in contact with the liquid surface, such as biochemical analyzer above the washing tank, level detection adjusting circuit 5, so that a suitable operating voltage level to Va, the sample can be calculated and the needle 4 corresponding to the capacitance value. 生化仪长时间运行后,可能造成样本针4堵塞,当样本针4 堵塞后,可变电容(针电容)明显增大,相应地,工作电压也会增大,设为Vb,在环境温度保持25°C不变的情况下,当Vb-Va大于某一个阈值设为Δ V, CPU 8就通过接口电路9输出堵塞信号14给控制板2,表示样本针4被堵塞了。 After prolonged running biochemical analyzer, sample needle 4 may cause clogging, clogging of the sample needle 4 when the variable capacitance (capacitance needle) significantly increased, accordingly, will increase the operating voltage, Vb is set, at ambient temperature 25 ° C in the case of the same, when Vb-Va larger than a certain threshold value is set to a Δ V, CPU 8 outputs a jam signal via the interface circuit 914 to the control panel 2, 4 represents a sample needle is blocked. [0043] 控制板2收到堵塞信号之后,发信息通知上位机3 (如PC),要求上位机3从人机界面通知操作者样本针4堵塞了,由此操作者就能够采取对应措施,比如进行强制冲洗等。 After [0043] congestion control panel 2 receives the signal, a message to notify the host computer 3 (e.g., PC), PC claim 3 inform the operator of the sample needle 4 from clogging the man-machine interface, whereby the operator can take the corresponding measures, such as forced flushing. 表1是具体的实验数据,样本针吸取一定量血清样本后静止放置,模拟针堵塞过程,模拟实验一中,样本针堵塞前后可变电容(针电容)分别为:25. 21pF、25. 63pF,变化量为0. 42pF, 堵塞前后工作电压分别为:0. 913VU. 862V,变化量为0. 949V ;模拟实验二中,样本针堵塞前后可变电容(针电容)分别为:25.86pF、26. 37pF,变化量为0. 51pF,堵塞前后工作电压分别为:1. 427V、2. 523V,变化量为1. 096V ;[0044] 表1样本针堵塞实验[0045] Table 1 is a specific experimental data draw a certain amount of the sample needle serum sample to stand still, a simulated needle plugging process, a simulation experiment, the sample needle clogging before and after the variable capacitance (capacitance-pin) are:. 25 21pF, 25 63pF. , the amount of change of 0. 42pF, respectively before and after the operating voltage is blocked: 0 913VU 862V, the amount of change of 0. 949V; II simulation before and after the sample needle clogging variable capacitance (capacitance-pin) are:.. 25.86pF, 26. 37pF, the change amount of 0. 51pF, respectively before and after the operating voltage clogging:.. 1 427V, 2 523V, the amount of change is 1. 096V; [0044] table 1 experimental sample needle blockage [0045]

Figure CN101004424BD00071

[0046] 由此可见,样本针堵塞前后可变电容(针电容)变化量以及工作电压变化量都较大。 [0046] Thus, before and after the sample needle clogging variable capacitance (capacitance-pin), and the amount of change in the operating voltage change amount are larger. [0047] 前面提到要求环境温度保持不变,这是因为样本可变电容(针电容)大小会随着环境温度变化而变化,两者近似线性关系,如图5所示,环境温度升高可变电容(针电容) 将增大,变化率约为0.03pF/°C。 [0047] The aforementioned requirements ambient temperature remains constant, because the sample variable capacitance (capacitance pin) sizes vary ambient temperature changes, the two approximate linear relationship, as shown, the ambient temperature increases 5 variable capacitance (capacitance needle) will increase, the rate of change is about 0.03pF / ° C. 其中,横坐标为环境温度(°C),纵坐标为可变电容(针电容)值(PF)。 Wherein the abscissa is temperature (° C), the ordinate is a variable capacitance (capacitance pin) value (PF). 曲线标注为环境温度分别在3°C、5°C、13°C、24°C与35°C条件下的可变电容(针电容)值。 A variable capacitance curve denoted respectively at 3 ° C, 5 ° C, 13 ° C, 24 ° C and 35 ° C conditions (pin capacitance) value of the ambient temperature. [0048] 根据工作电压与样本可变电容(针电容)的对应关系,环境温度升高将使得工作电压也增大,根据样本可变电容(针电容)与温度的变化率可以得到工作电压与温度的变化率,设为Vt/°c。 [0048] The working voltage variable capacitor with the sample (needle capacitance) corresponding relation between the ambient temperature increases will cause the operating voltage is increased, depending on the sample variable capacitance (capacitance needle) and rate of change of the temperature and operating voltage can be obtained temperature change rate, to Vt / ° c. [0049] 比如,在常温25°C下,样本针处于初始位置时,工作电压为Va,当样本针堵塞后, 工作电压增大为Vb,如果环境温度升高到了30°C,则工作电压应为:Vb+(30-2¾ *Vt。[0050] 这样就可能出现两种误判,一种是虽然样本针未堵塞,但因为环境温度的升高使得工作电压增大,当电压增大到一定程度,比如超过了前面提到的检测阈值Δν,则就会误判堵塞;另一种情况是,虽然样本针已经堵塞了,工作电压增大了,但因为环境温度降低使得工作电压又减小,这会使得工作电压变化不能超过检测阈值△ V,也同样会造成误判。[0051] 本方案中,利用温度传感电路6把当前的环境温度对应值输入到CPU 8,通过软件处理来解决温度的影响问题。假设样本针处于初始状态,即清洗干净且位于清洗池上方, 环境温度为Tl,此时液面检测电路工作电压为Va,当样本针堵塞等原 When [0049] For example, at room temperature 25 ° C, the sample needle is in the initial position, the working voltage Va, when the sample needle clogging, working voltage Vb is increased, if the ambient temperature rises to 30 ° C, the operating voltage should be: Vb + (30-2¾ * Vt [0050] Thus there are two possible misjudgment, although one is not clogged sample needle, but because of increasing ambient temperature so that the working voltage is increased, when the voltage increases to. to a certain extent, such as exceeding the detection threshold value Δν previously mentioned, the blockage will be false; another case, although the sample needle has been blocked, the operating voltage is increased, but because the ambient temperature is reduced so that the operating voltage and Save small, which would make the operating voltage can not exceed the detection threshold variations △ V, will also cause misjudgment. [0051] in the present embodiment, by the temperature sensing circuit 6 corresponding to the current ambient temperature value is input to the CPU 8, by software processing to solve the problem of the influence of temperature. Suppose the sample needle is in the initial state, i.e., clean and located above the washing tank, the ambient temperature Tl, the liquid level detection circuit voltage at this time was Va, when the original sample needle clogging 因使得样本可变电容(针电容)增大,工作电压也升高为Vb,并通过温度传感电路信号得到此时的环境温度为T2,因此,除去环境温度影响外,因堵塞造成的工作电压变化量,用V表示,应为:[0052] Such that the sample due to the variable capacitance (capacitance needles) is increased, the working voltage Vb is increased, the temperature sensing circuit and a signal obtained by the ambient temperature at that time is T2, and therefore, except for environmental temperature, due to clogging of the work a voltage change amount, expressed by V, should be: [0052]

Figure CN101004424BD00081

[0053] 当V大于Δ V,则判断样本针堵塞。 [0053] When V is greater than Δ V, it is determined that the sample needle clogging. [0054] 其更多实施例[0055] 上述实施例仅用于对本发明进行说明,而并非对本发明的保护范围的限制。 [0054] Further embodiments thereof [0055] The above-described embodiments are illustrative only of the present invention will be described, and not to limit the scope of the invention. 本领域的技术人员在本发明的启示下可以做出一些变通的设计,均属于本发明的保护范围,例如:[0056] 样本针也可以设计成可变电感、可变电阻等可变电阻抗,也可以设计成磁敏元件、 巨磁电阻等可变磁阻抗,等等;相应地,液面检测电路也可以改为电感检测电路、电阻检测电路、磁阻检测电路,等等,不再列举。 Those skilled in the art in light of the present invention may be made in a number of alternative designs, all fall within the scope of the present invention, for example: [0056] sample needle may be designed as a variable inductor, varistor variable impedance, may also be designed as a variable magnetic sensing element impedance, like giant magnetoresistance, and the like; accordingly, the liquid level detection circuit may be changed to inductance detecting circuit, the resistance detection circuit, a magnetoresistive sensor circuit, etc., do not then enumerated.

Claims (12)

1. 一种生化分析仪样本针堵塞检测装置,包括样本针G)、检测电路(1)、控制板0), 所述检测电路具有CPU(S)、接口电路(9),其特征是:所述样本针(4)为可变阻抗;所述检测电路(1)包括液面检测电路(5);所述样本针(4)作为可变阻抗接入到液面检测电路(5) 中,液面检测电路(5)检测所述可变阻抗的阻抗变化Vb-Va,并将检测信号输出至CPU(8), 当所述可变阻抗的阻抗变化Vb-Va大于某一个阈值设为M, CPU(S)就通过接口电路(9) 输出堵塞信号给控制板O),表示样本针(4)被堵塞了;其中Va为初始状态时液面检测电路工作电压,Vb为生化分析仪运行后的液面检测电路工作电压。 A biochemical analyzer sample needle clogging detecting means comprises a sample needle G), the detection circuit (1), the control panel O), the detection circuit having a CPU (S), an interface circuit (9), characterized in that: the sample needle (4) is a variable impedance; the detection circuit (1) comprises a level detection circuit (5); the sample needle (4) access to the liquid level detection circuit is used as variable impedance (5) detecting the change in impedance of the variable impedance level detection circuit (5) Vb-Va, and outputs a detection signal to the CPU (8), when a certain threshold value of the impedance of the variable impedance change Vb-Va is set large M, CPU (S) on via the interface circuit (9) outputs a jam signal to the control panel O), represents the sample needle (4) is blocked; wherein Va is a voltage level detection circuit initial state, Vb biochemical analyzer after running the operating voltage level detection circuit.
2.如权利要求1所述的生化分析仪样本针堵塞检测装置,其特征是:所述可变阻抗为可变电容。 The biochemical analyzer sample needle as claimed in claim 1, 2. The blockage detection unit, wherein: the variable impedance is a variable capacitance.
3.如权利要求2所述的生化分析仪样本针堵塞检测装置,其特征是:所述可变电容为双层同轴结构,内层(4a)与外层(4b)之间用绝缘材料(4c)隔绝,内层(4a)和外层(4b) 分别作为可变电容的两个极板。 3. The biochemical analyzer sample needle clogging detection apparatus according to claim 2, characterized in that: the variable capacitance is a double coaxial structure, with an insulating material between the inner layer (4a) and an outer layer (4b) (4c) isolated from the inner layer (4a) and an outer layer (4b), respectively, as the two plates of a variable capacitor.
4.如权利要求1或2或3所述的生化分析仪样本针堵塞检测装置,其特征是:所述液面检测电路(5)包括振荡源(51)、仪表放大器(52)和平衡电路,所述振荡源(51)所产生的信号(Vin) —路经平衡电路的第一支路输入到仪表放大器(52)的负输入端(V-),另一路经平衡电路的第二支路输入到仪表放大器的正输入端(V+),样本针内外两层分别作为可变电容(Cp)的两个极接入到平衡电路的第二支路中;所述平衡电路设定为当可变电容(Cp) 的电容值为特定值时,其输入到仪表放大器(5¾的正输入端(V+)和负输入端(V-)的信号保持平衡,当可变电容(Cp)的电容值偏离特定值时,其输入到仪表放大器(5¾的正输入端(V+)和负输入端(V-)的信号产生差异。 4. The biochemical analyzer sample needle blockage detection unit 1 or claim 2 or claim 3, wherein: said level detection circuit (5) comprises an oscillation source (51), an instrumentation amplifier (52) and balancing circuit , the oscillating signal source (51) generated (Vin) - balancing circuit path to the first branch input instrumentation amplifier (52) a negative input terminal (V-), a second further path balancing circuit branch instrumentation amplifier input to the positive input terminal (V +), the sample needle as two outer and inner layers respectively access to the second branch electrode balancing variable capacitance circuit (Cp); said balancing circuit is set when when a specific value of the variable capacitance value of the capacitance (Cp), which is input to the instrumentation amplifier (5¾ the positive input terminal (V +) and the negative input terminal (V-) of the signal balance, when the capacitance of the variable capacitance (Cp) of when the value deviates from a specific value, which is input to the instrumentation amplifier (5¾ the positive input terminal (V +) and the negative input terminal (V-) a difference signal.
5.如权利要求1或2或3所述的生化分析仪样本针堵塞检测装置,其特征是:还包括上位机(3),所述控制板⑵一端与检测电路⑴相连,另一端与上位机(3)相连;所述检测电路(1)中还包括有依次相连的A/D变换电路(7),所述A/D变换电路(7)的输入端与液面检测电路(5)相连。 5. biochemical analyzer sample needle 1 or claim 2 or claim 3 clogging detecting means, characterized by: further comprising a host computer (3), the control plate ⑵ ⑴ one end connected to the detection circuit, the other end of the upper machine (3) is connected; said detection circuit (1) further comprises sequentially connected with a a / D conversion circuit (7), the a / D conversion circuit (7) and the input of the level detection circuit (5) connected.
6.如权利要求5所述的生化分析仪样本针堵塞检测装置,其特征是:所述检测电路(1) 中还包括有温度传感电路(6),其输出端与A/D变换电路(7)的另一输入端相连;所述温度传感电路(6)检测所述样本针(4)所处环境的温度变化,并将温度信号输出至CPU(S);CPU(S)将因堵塞造成的工作电压变化量(V)按如下公式校正:其中Vt为可变阻抗工作电压与温度的变化率,Tl为初始状态环境温度,Va为初始状态时液面检测电路工作电压,Vb为生化分析仪运行后的液面检测电路工作电压,T2为通过温度传感电路信号得到的Vb时的环境温度; 当V大于阈值(Δν)则判断样本针堵塞。 An output coupled to A / D conversion circuit of the detection circuit (1) further comprises a temperature sensing circuit (6): as claimed in biochemical analyzer sample needle clogging detecting device according to claim 5, characterized in that (7) the other input terminal; (6) the sample needle detecting the temperature sensing circuit (4) in which the temperature changes in the environment, and the temperature signal to the CPU (S); CPU (S) to working voltage change amount due to clogging (V) corrected by the following formula: where Vt is the operating voltage of the variable impedance change rate and the temperature, Tl is an ambient temperature the initial state, Va is a voltage level detection circuit initial state, Vb a voltage level detection circuit of the biochemical analyzer operation, T2 is the ambient temperature by a temperature sensing circuit signal Vb obtained; if V is greater than the threshold value (Δν) determines that the sample needle clogging.
7. —种生化分析仪样本针堵塞检测方法,其特征是包括如下步骤: Α、将样本针(4)作为可变阻抗接入到液面检测电路(5)中;B、利用检测电路(5)检测所述可变阻抗的阻抗变化Vb-Va,并将检测信号输出;其中Va 为初始状态时液面检测电路工作电压,Vb为生化分析仪运行后的液面检测电路工作电压;C、根据输出的信号判断样本针是否堵塞:当所述可变阻抗的阻抗变化Vb-Va大于某一个阈值设为Δν,表示样本针被堵塞。 7. - Species biochemical analyzer sample needle clogging detecting method, characterized by comprising the steps of: [alpha], the sample needle (4) access to the liquid level detection circuit (5) as a variable impedance; B, using the detection circuit ( 5) detecting a change in impedance of the variable impedance Vb-Va, and outputs a detection signal; wherein Va is the initial state of the level detection circuit voltage, Vb is the biochemical analyzer operating voltage level detection circuit; C , based on a signal output from the sample needle clogging: when the impedance of the variable impedance change Vb-Va larger than a certain threshold value is set to a Δν, showing sample needle is blocked.
8.如权利要求7所述的生化分析仪样本针堵塞检测方法,其特征是:所述可变阻抗为可变电容。 The biochemical analyzer sample needle as claimed in claim 7, clogging detecting method, wherein: the variable impedance is a variable capacitance.
9.如权利要求7或8所述的生化分析仪样本针堵塞检测方法,其特征是步骤B中检测电路(5)检测所述可变阻抗的阻抗变化的方法包括如下步骤:Bi、利用振荡源(51)所产生信号(Vin);Β2、将所述信号一路经平衡电路的第一支路输入到仪表放大器(52)的负输入端(V-), 另一路经平衡电路的第二支路输入到仪表放大器的正输入端(V+);Β3、仪表放大器将检测到的信号输出;其中,样本针内外两层分别作为可变电容(Cp)的两个极接入到平衡电路的第二支路中;所述平衡电路设定为当可变电容(Cp)的电容值为特定值时,其输入到仪表放大器(52)的正输入端(V+)和负输入端(V-)的信号保持平衡,当可变电容(Cp)的电容值偏离特定值时,其输入到仪表放大器(5¾的正输入端(V+)和负输入端(V-)的信号产生差异。 Biochemical analyzer sample needle clogging detecting method as claimed in claim 7 or 8, wherein the step B, the detection circuit (5) The method of detecting the impedance of the variable impedance change comprises the steps of: Bi, use the oscillation a source (51) produced signal (Vin); Β2, one input signal to the instrumentation amplifier (52) a negative input terminal (V-) via a first leg of the balanced circuit, the balancing circuit further second path branch input to instrumentation amplifier positive input terminal (V +); Β3, instrumentation amplifier outputs a signal detected; wherein the outer and inner layers, respectively, the sample needle as two variable capacitors (Cp) to access the source balancing circuit a second branch; the balancing circuit is set when the variable capacitance (Cp) is a specific capacitance value, which is input to the instrumentation amplifier (52) positive input terminal (V +) and the negative input terminal (V- ) balanced signal, when the variable capacitance (capacitance value Cp) deviates from a specific value, which is input to the instrumentation amplifier (5¾ the positive input terminal (V +) and the negative input terminal (V-) a difference signal.
10.如权利要求7所述的生化分析仪样本针堵塞检测方法,其特征是在步骤B之后还包括如下步骤:将输出的检测信号依次输入到A/D变换电路(7)、CPU(S)、接口电路(9)、控制板O)、 上位机⑶。 10. The biochemical analyzer according to the sample needle clogging detection method of claim 7, wherein after the step B further comprises the step of: sequentially outputs the detection signal is input to the A / D conversion circuit (7), CPU (S ), an interface circuit (9), the control panel O), PC ⑶.
11.如权利要求7或8所述的生化分析仪样本针堵塞检测方法,其特征是:在步骤B中还检测环境温度,并将环境温度检测值依次输入到A/D变换电路(7)、 CPU(S)、接口电路(9)、控制板(2)、上位机(3)。 1. The biochemical analyzer sample needle 7 or claim 8, clogging detecting method, wherein: in step B further detected ambient temperature, and the ambient temperature detection value sequentially input to the A / D conversion circuit (7) , CPU (S), an interface circuit (9), a control board (2), PC (3).
12.如权利要求11所述的生化分析仪样本针堵塞检测方法,其特征是:将因堵塞造成的工作电压变化量V按如下公式校正: 12. The biochemical analyzer sample needle 11 of the clogging detecting method as claimed in claim, wherein: the voltage change amount of work due to clogging corrected by the following formula V:
Figure CN101004424BC00031
其中Vt为可变阻抗工作电压与温度的变化率,Tl为初始状态环境温度,Va为初始状态时液面检测电路工作电压,Vb为可变阻抗改变后的液面检测电路工作电压,T2为通过温度传感电路信号得到的Vb时的环境温度;当V大于阈值ΔV则判断样本针堵塞。 Where Vt is a variable impedance rate of change of the operating voltage and temperature, Tl is an ambient temperature an initial state, Va is the initial state when the voltage level detection circuit, Vb of the variable impedance circuit to change the voltage level detection, T2 is by a temperature sensing circuit signals obtained when the ambient temperature Vb; V is greater than the threshold value ΔV when it is determined that the sample needle clogging.
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