CA3208720A1 - Compositions and methods for producing rebaudioside d - Google Patents

Compositions and methods for producing rebaudioside d Download PDF

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Publication number
CA3208720A1
CA3208720A1 CA3208720A CA3208720A CA3208720A1 CA 3208720 A1 CA3208720 A1 CA 3208720A1 CA 3208720 A CA3208720 A CA 3208720A CA 3208720 A CA3208720 A CA 3208720A CA 3208720 A1 CA3208720 A1 CA 3208720A1
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CA
Canada
Prior art keywords
residue
rebaudioside
acid sequence
amino acid
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3208720A
Other languages
English (en)
French (fr)
Inventor
Kyle Eugene ROBERTS
Alexandre Zanghellini
Daniela GRABS
Niklas Dalgas KRISTIANSEN
James J. Havranek
Yih-En Andrew BAN
Ashwini DEVKOTA
Mark NANCE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ARZEDA CORP
Original Assignee
ARZEDA CORP
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ARZEDA CORP filed Critical ARZEDA CORP
Publication of CA3208720A1 publication Critical patent/CA3208720A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CA3208720A 2021-02-17 2022-02-17 Compositions and methods for producing rebaudioside d Pending CA3208720A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163150515P 2021-02-17 2021-02-17
US63/150,515 2021-02-17
PCT/US2022/016820 WO2022178145A1 (en) 2021-02-17 2022-02-17 Compositions and methods for producing rebaudioside d

Publications (1)

Publication Number Publication Date
CA3208720A1 true CA3208720A1 (en) 2022-08-25

Family

ID=82931006

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3208720A Pending CA3208720A1 (en) 2021-02-17 2022-02-17 Compositions and methods for producing rebaudioside d

Country Status (11)

Country Link
US (1) US20240368661A1 (https=)
EP (1) EP4294934A4 (https=)
JP (1) JP2024507361A (https=)
KR (1) KR20240010448A (https=)
CN (1) CN117616129A (https=)
BR (1) BR112023016512A2 (https=)
CA (1) CA3208720A1 (https=)
CO (1) CO2023010756A2 (https=)
MX (1) MX2023009628A (https=)
PE (1) PE20240694A1 (https=)
WO (1) WO2022178145A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3266431A1 (en) * 2022-09-02 2024-03-07 Arzeda Corp. Compositions and methods for producing rebaudioside m
CN115947788B (zh) * 2022-10-12 2023-08-29 东北农业大学 色氨酸和亮氨酸跨链交互作用β-发卡抗菌肽WLF及制备方法和应用
EP4608984A2 (en) * 2022-10-26 2025-09-03 Arzeda Corp. Enzymatically mediated reactive crystallization of steviol glycosides
EP4724575A1 (en) * 2023-06-06 2026-04-15 Arzeda Corp. Compositions and methods for producing rebaudioside d and rebaudioside m
WO2026006475A1 (en) * 2024-06-25 2026-01-02 Arzeda Corp. Compositions and methods for glycosylation of steviol glycosides
CN119799672B (zh) * 2025-01-15 2025-07-25 皖西学院 一种糖基转移酶突变体及其在合成红景天苷中的应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9752174B2 (en) * 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
PE20200291A1 (es) * 2017-05-15 2020-02-05 Purecircle Usa Inc Glicosidos de esteviol de alta pureza
IL280302B2 (en) * 2018-07-30 2025-09-01 Codexis Inc Engineered glycosyltransferases and methods for glycosylation of steviol glycoside
EP3973069A4 (en) * 2019-05-23 2023-09-13 Arzeda Corp. COMPOSITIONS AND METHODS FOR PRODUCING STEVIOL GLYCOSIDES

Also Published As

Publication number Publication date
PE20240694A1 (es) 2024-04-10
EP4294934A4 (en) 2025-05-21
CN117616129A (zh) 2024-02-27
BR112023016512A2 (pt) 2023-10-24
JP2024507361A (ja) 2024-02-19
EP4294934A1 (en) 2023-12-27
MX2023009628A (es) 2024-01-25
KR20240010448A (ko) 2024-01-23
US20240368661A1 (en) 2024-11-07
CO2023010756A2 (es) 2024-01-15
WO2022178145A1 (en) 2022-08-25

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