CA3154506A1 - Systems and devices for electroporation of cell-containing fluid - Google Patents

Systems and devices for electroporation of cell-containing fluid Download PDF

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Publication number
CA3154506A1
CA3154506A1 CA3154506A CA3154506A CA3154506A1 CA 3154506 A1 CA3154506 A1 CA 3154506A1 CA 3154506 A CA3154506 A CA 3154506A CA 3154506 A CA3154506 A CA 3154506A CA 3154506 A1 CA3154506 A1 CA 3154506A1
Authority
CA
Canada
Prior art keywords
electroporation
chamber
cartridge
electrode
volume
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3154506A
Other languages
English (en)
French (fr)
Inventor
Steven Huei YEO
Siu Wee Hon
Han Wei
Yvonne PECK
Way Xuang LEE
Benyong SHI
Chee Wai Chan
Beng Heng Lim
Dhanaraj SHANMUGAM
Stacey TEO
Jun Yan Tham
Kok Shyong CHONG
Li Yang LIM
Michael Gordon
Nektaria ANDRONIKOU
Chris Rossman
Xavier De Mollerat Du Jeu
Ulrich Forke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Holdings Pty Ltd
Life Technologies Corp
Original Assignee
Life Technologies Holdings Pty Ltd
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Holdings Pty Ltd, Life Technologies Corp filed Critical Life Technologies Holdings Pty Ltd
Publication of CA3154506A1 publication Critical patent/CA3154506A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/44—Multiple separable units; Modules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Thermal Sciences (AREA)
  • Computer Hardware Design (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
CA3154506A 2019-10-25 2020-10-23 Systems and devices for electroporation of cell-containing fluid Pending CA3154506A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962926078P 2019-10-25 2019-10-25
US62/926,078 2019-10-25
PCT/US2020/057138 WO2021081381A1 (en) 2019-10-25 2020-10-23 Systems and devices for electroporation of cell-containing fluid

Publications (1)

Publication Number Publication Date
CA3154506A1 true CA3154506A1 (en) 2021-04-29

Family

ID=73402180

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3154506A Pending CA3154506A1 (en) 2019-10-25 2020-10-23 Systems and devices for electroporation of cell-containing fluid

Country Status (10)

Country Link
US (2) US20210123009A1 (https=)
EP (1) EP4048776A1 (https=)
JP (2) JP7784374B2 (https=)
KR (1) KR20220088469A (https=)
CN (2) CN214361426U (https=)
AU (1) AU2020370481B2 (https=)
BR (1) BR112022007622A2 (https=)
CA (1) CA3154506A1 (https=)
DE (1) DE202020106067U1 (https=)
WO (1) WO2021081381A1 (https=)

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* Cited by examiner, † Cited by third party
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US12097345B2 (en) * 2018-06-01 2024-09-24 Penumbra, Inc. Infusion catheter and methods of use
EP4048776A1 (en) * 2019-10-25 2022-08-31 Life Technologies Corporation Systems and devices for electroporation of cell-containing fluid
USD943760S1 (en) * 2020-05-11 2022-02-15 Maxcyte, Inc. Electroporation processing assembly
USD944410S1 (en) 2020-05-11 2022-02-22 Maxcyte, Inc. Electroporation processing assembly
USD965170S1 (en) * 2020-10-23 2022-09-27 Life Technologies Corporation Electroporation device
US20230110090A1 (en) * 2021-10-08 2023-04-13 Life Technologies Holdings Pte Limited System and Method of Electroporation Protocol Optimization
TW202400835A (zh) 2022-03-09 2024-01-01 荷蘭商Ａｓｍ Ｉｐ私人控股有限公司 反應器系統、控制液體源的溫度之方法、及經調適以容納容器的裝置
WO2023230253A1 (en) * 2022-05-27 2023-11-30 Sqz Biotechnologies Company Cartridges and devices for use in a system for delivery of a payload into a cell
CN117431158A (zh) * 2022-07-14 2024-01-23 苏州壹达生物科技有限公司 一种流式电穿孔装置
US20260092248A1 (en) 2022-09-15 2026-04-02 Life Technologies Corporation Bioreactors operable in static and dynamic modes and methods of use
KR102609307B1 (ko) * 2022-10-24 2023-12-04 티큐브잇(주) 유전자 형질 감염 장치
CN115722292B (zh) * 2022-11-22 2023-12-29 山东省煤田地质局第一勘探队 一种地下水自然资源调查用分类收集装置及使用方法
CN120882852A (zh) * 2023-06-21 2025-10-31 隆萨科隆有限公司 多腔室电穿孔盒及其使用方法
WO2026043806A2 (en) 2024-08-19 2026-02-26 Life Technologies Corporation Systems, methods, and assemblies for cell processing
CN120028438B (zh) * 2025-04-23 2025-08-26 晋城市鑫环球铸造有限公司 一种基于球墨铸铁管生产的探伤检测设备及方法

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US5545130A (en) * 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US5650305A (en) * 1996-05-15 1997-07-22 Health Research Inc. Method to produce hybrid cells by electrofusion
US6150148A (en) 1998-10-21 2000-11-21 Genetronics, Inc. Electroporation apparatus for control of temperature during the process
US6969604B1 (en) * 2003-06-20 2005-11-29 Yakovenko Sergey A Electroporation chamber
US20050282283A1 (en) * 2004-04-19 2005-12-22 Laura Vozza-Brown Electroporation apparatus and methods
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CA3017108A1 (en) * 2015-03-12 2016-09-15 The Trustees Of The University Of Pennsylvania System, method, and device for high-throughput, automated culturing of genetically modified organisms
JP6660057B2 (ja) 2015-12-11 2020-03-04 国立大学法人豊橋技術科学大学 エレクトロポレーション用電極及び外来物質導入装置
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Also Published As

Publication number Publication date
AU2020370481A1 (en) 2022-05-26
WO2021081381A1 (en) 2021-04-29
JP2026040486A (ja) 2026-03-09
US20210123009A1 (en) 2021-04-29
EP4048776A1 (en) 2022-08-31
AU2020370481B2 (en) 2026-04-02
CN214361426U (zh) 2021-10-08
KR20220088469A (ko) 2022-06-27
JP2023501142A (ja) 2023-01-18
DE202020106067U1 (de) 2020-12-04
JP7784374B2 (ja) 2025-12-11
BR112022007622A2 (pt) 2022-10-11
US20240263124A1 (en) 2024-08-08
CN112708552A (zh) 2021-04-27

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