CA3140183A1 - Two-dimensional lc-ms/ms systems - Google Patents

Two-dimensional lc-ms/ms systems Download PDF

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Publication number
CA3140183A1
CA3140183A1 CA3140183A CA3140183A CA3140183A1 CA 3140183 A1 CA3140183 A1 CA 3140183A1 CA 3140183 A CA3140183 A CA 3140183A CA 3140183 A CA3140183 A CA 3140183A CA 3140183 A1 CA3140183 A1 CA 3140183A1
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CA
Canada
Prior art keywords
column
solvent
sample
analytes
acetonitrile
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3140183A
Other languages
English (en)
French (fr)
Inventor
Wei-Lien Chuang
Felipe Gazos LOPES
Joshua Pacheco
Gerard Sanderink
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genzyme Corp
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of CA3140183A1 publication Critical patent/CA3140183A1/en
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/468—Flow patterns using more than one column involving switching between different column configurations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B01D15/1878—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series for multi-dimensional chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/322—Normal bonded phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N30/14—Preparation by elimination of some components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G01N30/463—Flow patterns using more than one column with serial coupling of separation columns for multidimensional chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/89—Inverse chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
    • G01N2405/08—Sphingolipids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders
    • G01N2800/044—Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Endocrinology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Electron Tubes For Measurement (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA3140183A 2019-05-31 2020-05-29 Two-dimensional lc-ms/ms systems Pending CA3140183A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962855636P 2019-05-31 2019-05-31
US62/855,636 2019-05-31
PCT/US2020/035231 WO2020243502A1 (en) 2019-05-31 2020-05-29 Two-dimensional lc-ms/ms systems

Publications (1)

Publication Number Publication Date
CA3140183A1 true CA3140183A1 (en) 2020-12-03

Family

ID=72193559

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3140183A Pending CA3140183A1 (en) 2019-05-31 2020-05-29 Two-dimensional lc-ms/ms systems

Country Status (14)

Country Link
US (2) US12292424B2 (https=)
EP (2) EP3976218B1 (https=)
JP (3) JP7568648B2 (https=)
KR (2) KR20260040656A (https=)
CN (2) CN119861166A (https=)
AU (1) AU2020282831B2 (https=)
CA (1) CA3140183A1 (https=)
DK (1) DK3976218T3 (https=)
ES (1) ES3026686T3 (https=)
HU (1) HUE071194T2 (https=)
IL (2) IL288497B1 (https=)
PL (1) PL3976218T3 (https=)
TW (1) TWI862597B (https=)
WO (1) WO2020243502A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120254137B (zh) * 2025-06-05 2026-04-14 科诺美(北京)科技有限公司 辅酶q10的检测方法及其应用
CN120820662B (zh) * 2025-09-17 2025-11-18 岛津企业管理（中国）有限公司 一种同步分析检测水产品中代谢物和脂质的方法

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* Cited by examiner, † Cited by third party
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EP0998229A4 (en) * 1997-06-17 2001-04-04 Cool Laser Optics Inc METHOD AND APPARATUS FOR REGULATING BIOLOGICAL TISSUE TEMPERATURE BY SIMULTANEOUS IRRADIATION
WO2000045929A1 (en) * 1999-02-08 2000-08-10 Admetric Biochem Inc. Chromatographic system with pre-detector eluent switching
JP2003028849A (ja) 2001-07-10 2003-01-29 Hiromasa Tojo 自動化脂質組成一斉分析装置
JP2004184215A (ja) 2002-12-03 2004-07-02 Shimadzu Corp アクリルアミドの分析方法及び装置
EP1635174A1 (en) 2003-02-28 2006-03-15 Japan Science and Technology Agency Method of separating sugar from compound having glycoside bond, sugar separation system, sugar separation agent kit, standardized sample for sugar separation and assessment system
KR100614099B1 (ko) 2005-11-25 2006-08-22 한국과학기술연구원 뇨 또는 플라즈마 시료 중의 디에이치이에이 설페이트의검출방법
JP4790435B2 (ja) * 2006-02-03 2011-10-12 株式会社日立ハイテクノロジーズ ３次元液体クロマトグラフィ
US20080183401A1 (en) * 2007-01-29 2008-07-31 Teledyne Isco, Inc. Apparatuses and methods for wireless monitoring and control of enviromental sampling and chromatographic apparatuses
JP5054439B2 (ja) 2007-06-15 2012-10-24 株式会社日立ハイテクノロジーズ イオン交換および順相カラムによる２次元液体クロマトグラフ
DE102008013500A1 (de) * 2008-03-10 2009-09-17 Evonik Degussa Gmbh Neue chirale Selektoren und stationäre Phasen zur Trennung von Enantiomerengemischen
WO2012129076A1 (en) * 2011-03-23 2012-09-27 Idex Health & Science Llc Valve and splitting system for multi-dimensional liquid analysis
ES2731920T3 (es) 2013-05-14 2019-11-19 Centogene Ag Método para el diagnóstico de la enfermedad de Niemann-Pick
LT3004896T (lt) * 2013-06-07 2020-01-10 Genzyme Corporation Rūgštinės sfingomielinazės sutrikimų žymuo ir jo panaudojimas
JP6385103B2 (ja) * 2014-03-31 2018-09-05 株式会社島津製作所 順相・逆相カラムを備えた超臨界流体クロマトグラフとそれを用いた分析方法
KR101574272B1 (ko) * 2014-08-20 2015-12-07 한국표준과학연구원 하트 컷 방법을 위한 이차원 액체 크로마토그래피 시스템
US20160195502A1 (en) * 2014-12-31 2016-07-07 Castle Medical, LLC Method for chiral separation of methamphetamine and amphatamine enantiomers
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GB201601667D0 (en) 2016-01-29 2016-03-16 Ge Healthcare Bio Sciences Ab Improvements in and relating to liquid fraction collectors
JP6716087B2 (ja) 2016-03-25 2020-07-01 公立大学法人大阪 分析方法及び分析装置
TWI613445B (zh) 2016-04-01 2018-02-01 行政院農業委員會農業藥物毒物試驗所 搭配質譜影像分析檢驗農藥殘留之方法及其系統
US20180306797A1 (en) 2017-04-24 2018-10-25 Washington University Methods of detecting ceramide
HUE057483T2 (hu) 2017-06-02 2022-05-28 Genzyme Corp Eljárások kis gyakoriságú polipeptidek abszolút kvantifikálására tömeg-spektrometria alkalmazásával
KR20250025051A (ko) * 2017-08-24 2025-02-20 사노피 산성 스핑고미엘린분해효소 결핍증 환자에서의 비정상적 골병태의 치료
CN109187822A (zh) 2018-11-22 2019-01-11 上海烟草集团有限责任公司 一种中心切割二维色谱质谱联用分析烟草中农残的方法

Also Published As

Publication number Publication date
JP7804023B2 (ja) 2026-01-21
PL3976218T3 (pl) 2025-06-09
AU2020282831B2 (en) 2026-03-05
JP2026062952A (ja) 2026-04-10
ES3026686T3 (en) 2025-06-11
EP3976218B1 (en) 2025-02-19
AU2020282831A1 (en) 2021-12-16
IL288497B1 (en) 2026-04-01
KR20260040656A (ko) 2026-03-25
EP4560320A3 (en) 2025-08-13
JP7568648B2 (ja) 2024-10-16
IL326850A (en) 2026-04-01
IL288497A (en) 2022-01-01
JP2025000908A (ja) 2025-01-07
EP4560320A2 (en) 2025-05-28
CN119861166A (zh) 2025-04-22
KR20220016103A (ko) 2022-02-08
US20220252559A1 (en) 2022-08-11
DK3976218T3 (da) 2025-05-05
JP2022534363A (ja) 2022-07-29
CN114364980B (zh) 2024-12-27
KR102936574B1 (ko) 2026-03-09
WO2020243502A1 (en) 2020-12-03
CN114364980A (zh) 2022-04-15
TW202111322A (zh) 2021-03-16
TWI862597B (zh) 2024-11-21
US12292424B2 (en) 2025-05-06
US20250327780A1 (en) 2025-10-23
HUE071194T2 (hu) 2025-08-28
EP3976218A1 (en) 2022-04-06

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