CA3132197A1 - Rna-guided dna integration using tn7-like transposons - Google Patents

Rna-guided dna integration using tn7-like transposons Download PDF

Info

Publication number
CA3132197A1
CA3132197A1 CA3132197A CA3132197A CA3132197A1 CA 3132197 A1 CA3132197 A1 CA 3132197A1 CA 3132197 A CA3132197 A CA 3132197A CA 3132197 A CA3132197 A CA 3132197A CA 3132197 A1 CA3132197 A1 CA 3132197A1
Authority
CA
Canada
Prior art keywords
transposon
crispr
cas
nucleic acid
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3132197A
Other languages
English (en)
French (fr)
Inventor
Samuel Henry Sternberg
Sanne Eveline Klompe
Phuc Hong VO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Columbia University in the City of New York
Original Assignee
Columbia University in the City of New York
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Columbia University in the City of New York filed Critical Columbia University in the City of New York
Publication of CA3132197A1 publication Critical patent/CA3132197A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Physiology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA3132197A 2019-03-07 2020-03-06 Rna-guided dna integration using tn7-like transposons Pending CA3132197A1 (en)

Applications Claiming Priority (19)

Application Number Priority Date Filing Date Title
US201962815187P 2019-03-07 2019-03-07
US62/815,187 2019-03-07
US201962822544P 2019-03-22 2019-03-22
US62/822,544 2019-03-22
US201962845218P 2019-05-08 2019-05-08
US62/845,218 2019-05-08
US201962855814P 2019-05-31 2019-05-31
US62/855,814 2019-05-31
US201962866270P 2019-06-25 2019-06-25
US62/866,270 2019-06-25
US201962873455P 2019-07-12 2019-07-12
US62/873,455 2019-07-12
US201962875772P 2019-07-18 2019-07-18
US62/875,772 2019-07-18
US201962884600P 2019-08-08 2019-08-08
US62/884,600 2019-08-08
US201962902171P 2019-09-18 2019-09-18
US62/902,171 2019-09-18
PCT/US2020/021568 WO2020181264A1 (en) 2019-03-07 2020-03-06 Rna-guided dna integration using tn7-like transposons

Publications (1)

Publication Number Publication Date
CA3132197A1 true CA3132197A1 (en) 2020-09-10

Family

ID=72334850

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3132197A Pending CA3132197A1 (en) 2019-03-07 2020-03-06 Rna-guided dna integration using tn7-like transposons

Country Status (12)

Country Link
US (3) US10947534B2 (https=)
EP (1) EP3935179A4 (https=)
JP (1) JP7733576B2 (https=)
KR (1) KR20210149060A (https=)
CN (1) CN113795587A (https=)
AU (1) AU2020232850B2 (https=)
BR (1) BR112021017655A2 (https=)
CA (1) CA3132197A1 (https=)
IL (1) IL286089A (https=)
SG (1) SG11202109550TA (https=)
WO (1) WO2020181264A1 (https=)
ZA (1) ZA202106516B (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112852921A (zh) * 2021-03-16 2021-05-28 中国科学院长春应用化学研究所 一种基于即时检测试纸条的核酸检测方法、检测探针及其试剂盒

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019090175A1 (en) 2017-11-02 2019-05-09 Arbor Biotechnologies, Inc. Novel crispr-associated transposon systems and components
US20220372521A1 (en) * 2019-08-14 2022-11-24 The Trustees Of Columbia University In The City Of New York Rna-guided dna integration and modification
AU2020373064A1 (en) * 2019-11-01 2022-05-26 Massachusetts Institute Of Technology Type I-B CRISPR-associated transposase systems
CA3171941A1 (en) * 2020-03-16 2021-09-23 Joseph E. Peters Compositions and methods comprising improved guide rnas
WO2021195532A1 (en) * 2020-03-27 2021-09-30 The Trustees Of Columbia University In The City Of New York Genome engineering using crispr rna-guided integrases
US20240287486A1 (en) * 2020-04-24 2024-08-29 University Of Massachusetts Improved cas 12a/nls mediated therapeutic gene editing platforms
US20250032629A1 (en) * 2020-05-19 2025-01-30 Anygen Co., Ltd. Novel nucleolin-binding peptide and use thereof
CN116323971A (zh) * 2020-08-18 2023-06-23 Illumina公司 核酸的序列特异性靶向转座和选择以及分选
EP4217499A4 (en) * 2020-09-24 2025-02-12 Metagenomi, Inc. Systems and methods for transposing cargo nucleotide sequences
CA3194645A1 (en) * 2020-10-08 2022-04-14 The Broad Institute, Inc. Type i crispr-associated transposase systems
WO2022147321A1 (en) * 2020-12-30 2022-07-07 The Broad Institute, Inc. Type i-b crispr-associated transposase systems
WO2022150651A1 (en) * 2021-01-07 2022-07-14 The Broad Institute, Inc. Dna nuclease guided transposase compositions and methods of use thereof
WO2022162623A1 (en) * 2021-01-28 2022-08-04 Arbor Biotechnologies, Inc. Crispr-associated transposon systems and methods of using same
US20240301445A1 (en) * 2021-01-28 2024-09-12 Arbor Biotechnologies, Inc. Crispr-associated transposon systems and methods of using same
CN115369098A (zh) * 2021-05-17 2022-11-22 中国科学院分子植物科学卓越创新中心 一种新型crispr相关转座酶
IL309148A (en) * 2021-06-07 2024-02-01 Univ Columbia Crispr-transposon systems for dna modification
CA3223080A1 (en) * 2021-06-17 2022-12-22 Samuel Henry Sternberg Systems, methods, and components for rna-guided effector recruitment
US20230159914A1 (en) * 2021-06-24 2023-05-25 Digenomix Corporation Methods for reconstructing single cell genome
WO2023004422A2 (en) * 2021-07-22 2023-01-26 Arbor Biotechnologies, Inc. Crispr-associated transposon systems and methods of using same
US20250066819A1 (en) * 2021-08-16 2025-02-27 Board Of Regents, The University Of Texas System Crispr-associated transposons and uses thereof
EP4399312A4 (en) * 2021-09-08 2025-09-03 Metagenomi Inc SYSTEMS AND METHODS FOR TRANSPOSITION OF CHARGED NUCLEOTIDE SEQUENCES
TWI821883B (zh) * 2022-01-26 2023-11-11 國立清華大學 大腸桿菌基因編輯系統及其基因編輯方法
US20250197458A1 (en) * 2022-02-09 2025-06-19 Cornell University Adaptations for high efficiency i-f3-crispr-cas systems for guide rna-directed transposition in human cells
CA3244789A1 (en) * 2022-02-23 2023-08-31 Metagenomi Therapeutics, Inc. FUSE PROTEINS
MX2024010333A (es) * 2022-02-23 2024-08-30 Metagenomi Inc Sistemas y metodos para transponer secuencias de nucleotidos de carga.
CN114457100B (zh) * 2022-03-03 2023-06-09 江南大学 一种基于CRISPR/Cpf1的大肠杆菌基因编辑系统及其应用
US20250237648A1 (en) * 2022-04-13 2025-07-24 Baylor College Of Medicine Bacterial biosensors for diagnosing and treating inflammation
WO2023204594A1 (ko) * 2022-04-19 2023-10-26 서울대학교산학협력단 태그멘테이션을 이용한 벡터 삽입위치 검출 및 클론 정량 방법
WO2023205267A1 (en) * 2022-04-20 2023-10-26 The Administrators Of The Tulane Educational Fund Enhanced cell-free bacteriophage synthesis by genetic modulation of bacterial transcription/translation machinery (txtl) machinery
US20250290093A1 (en) * 2022-04-29 2025-09-18 Cz Biohub Sf, Llc High capacity lentiviral vectors
EP4536817A1 (en) 2022-06-07 2025-04-16 Institut National de la Recherche pour l'Agriculture, l'Alimentation et l'Environnement Attp mv4-derived site-specific recombination and its use for integration of sequence of interest
EP4536834A2 (en) * 2022-06-08 2025-04-16 North Carolina State University Recombinant type i-f3 transposon-associated crispr-cas systems and methods of use
EP4569120A2 (en) * 2022-06-13 2025-06-18 The Trustees Of Columbia University In The City Of New York Crispr-transposon systems for dna modification
CN118599809A (zh) * 2022-07-07 2024-09-06 山东舜丰生物科技有限公司 Cas酶及其应用
KR102793465B1 (ko) * 2022-10-19 2025-04-10 성균관대학교산학협력단 Tn7-유사 타입 CrisprCAS를 이용한 코리네박테리움 글루타미쿰(Corynebacterium glutamicum)균 유전체 내 유전자 삽입 시스템
JP2026500148A (ja) * 2022-12-01 2026-01-06 アーク リサーチ インスティテュート 核酸操作のためのプログラム可能なｄｎａトランスポザーゼ
WO2024124048A1 (en) * 2022-12-07 2024-06-13 The Trustees Of Columbia University In The City Of New York Systems and methods for rna-guided dna integration
CN116162640A (zh) * 2023-01-30 2023-05-26 态创生物科技(广州)有限公司 大肠杆菌Rosetta菌株及其在催化合成α-熊果苷中的应用
EP4665406A1 (en) * 2023-02-14 2025-12-24 The Trustees of Columbia University in the City of New York Crispr-transposon systems and components
CN119842702B (zh) * 2023-10-17 2025-11-28 清华大学 一种工程改造的dear核酸操纵系统的制备方法
US20250257365A1 (en) * 2024-02-09 2025-08-14 California Institute Of Technology Targeted DNA Integration in Plants by CRISPR-Associated Transposases (CASTs)
CN118460583B (zh) * 2024-07-15 2024-10-29 苏州左旋星生物科技有限公司 用于靶向大肠杆菌多基因位点编辑的载体及其应用
CN119752847B (zh) * 2024-12-26 2025-12-05 崖州湾国家实验室 一种高温耐受型的TnpB核酸酶在核酸检测及基因编辑中的应用

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5451513A (en) 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
US5576198A (en) 1993-12-14 1996-11-19 Calgene, Inc. Controlled expression of transgenic constructs in plant plastids
US5545817A (en) 1994-03-11 1996-08-13 Calgene, Inc. Enhanced expression in a plant plastid
US5545818A (en) 1994-03-11 1996-08-13 Calgene Inc. Expression of Bacillus thuringiensis cry proteins in plant plastids
ATE374821T1 (de) 1997-02-20 2007-10-15 Univ Johns Hopkins Med Mutationen in atp-abhängigen transpositionsproteinen die die zielortspezifität reduzieren
WO1999005295A1 (en) 1997-07-25 1999-02-04 Thomas Jefferson University Composition and method for targeted integration into cells
FI20030771A7 (fi) * 2003-05-21 2004-11-22 Helsingin Yliopisto Menetelmä toksisten syanobakteerien määrittämiseksi
US7608434B2 (en) 2004-08-04 2009-10-27 Wisconsin Alumni Research Foundation Mutated Tn5 transposase proteins and the use thereof
AU2008223544B2 (en) * 2007-03-02 2014-06-05 Dupont Nutrition Biosciences Aps Cultures with improved phage resistance
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
HUE041436T2 (hu) 2009-12-10 2019-05-28 Univ Minnesota Tal-effektor-közvetített DNS-módosítás
US9109225B2 (en) * 2012-09-14 2015-08-18 New York University Engineered transposon for facile construction of a random protein domain insertion library
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
WO2014194190A1 (en) 2013-05-30 2014-12-04 The Penn State Research Foundation Gene targeting and genetic modification of plants via rna-guided genome editing
WO2015148680A1 (en) 2014-03-25 2015-10-01 Ginkgo Bioworks, Inc. Methods and genetic systems for cell engineering
US10927384B2 (en) * 2014-04-09 2021-02-23 Dna Twopointo Inc. DNA vectors, transposons and transposases for eukaryotic genome modification
AU2015294354B2 (en) * 2014-07-21 2021-10-28 Illumina, Inc. Polynucleotide enrichment using CRISPR-Cas systems
WO2016025759A1 (en) 2014-08-14 2016-02-18 Shen Yuelei Dna knock-in system
WO2016057951A2 (en) 2014-10-09 2016-04-14 Life Technologies Corporation Crispr oligonucleotides and gene editing
EP3889260A1 (en) * 2014-12-12 2021-10-06 The Broad Institute, Inc. Protected guide rnas (pgrnas)
KR20250171479A (ko) * 2015-03-31 2025-12-08 소흠, 인코포레이티드 세포 또는 유기체의 게놈으로의 ＤＮＡ 서열의 표적화 혼입을 위한 Ｃａｓ 9 레트로바이러스 인테그라제 시스템 및 Ｃａｓ 9 재조합효소 시스템
CN107667173A (zh) * 2015-05-06 2018-02-06 斯尼普技术有限公司 改变微生物种群和改善微生物群
KR20220139447A (ko) * 2015-05-29 2022-10-14 노쓰 캐롤라이나 스테이트 유니버시티 크리스퍼 핵산을 사용하여 박테리아, 고세균, 조류 및 효모를 스크리닝하는 방법
GB201514510D0 (en) * 2015-08-14 2015-09-30 Nemesis Bioscience Ltd Delivery vehicle
US10004432B2 (en) 2015-09-01 2018-06-26 Qualcomm Incorporated Pixel receiver with capacitance cancellation for ultrasonic imaging apparatus
US11091780B2 (en) * 2015-09-18 2021-08-17 The Regents Of The University Of California Methods for autocatalytic genome editing and neutralizing autocatalytic genome editing and compositions thereof
CA3001312A1 (en) 2015-10-08 2017-04-13 Dna2.0, Inc. Dna vectors, transposons and transposases for eukaryotic genome modification
AU2016371903B2 (en) * 2015-12-16 2023-10-19 Syngenta Crop Protection Ag Genetic regions and genes associated with increased yield in plants
AU2016380351B2 (en) * 2015-12-29 2023-04-06 Monsanto Technology Llc Novel CRISPR-associated transposases and uses thereof
GB201602473D0 (en) * 2016-02-11 2016-03-30 Horizon Discovery Ltd Replicative transposon system
JOP20190166A1 (ar) 2017-01-05 2019-07-02 Univ Texas استراتيجية مثلى من أجل تعديلات تخطي إكسون باستخدام crispr/cas9 مع متواليات توجيه ثلاثي
WO2018152244A1 (en) * 2017-02-14 2018-08-23 Seqwell, Inc. Compositions and methods for sequencing nucleic acids
US20200377881A1 (en) * 2017-03-24 2020-12-03 President And Fellows Of Harvard College Methods of Genome Engineering by Nuclease-Transposase Fusion Proteins
ES2980050T3 (es) * 2017-06-08 2024-09-27 Univ Osaka Método para fabricar células eucariotas editadas con ADN
WO2019090175A1 (en) * 2017-11-02 2019-05-09 Arbor Biotechnologies, Inc. Novel crispr-associated transposon systems and components
WO2019126774A1 (en) * 2017-12-22 2019-06-27 The Broad Institute, Inc. Novel crispr enzymes and systems
CA3124110A1 (en) * 2018-12-17 2020-06-25 The Broad Institute, Inc. Crispr-associated transposase systems and methods of use thereof
AU2020373064A1 (en) * 2019-11-01 2022-05-26 Massachusetts Institute Of Technology Type I-B CRISPR-associated transposase systems

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112852921A (zh) * 2021-03-16 2021-05-28 中国科学院长春应用化学研究所 一种基于即时检测试纸条的核酸检测方法、检测探针及其试剂盒
CN112852921B (zh) * 2021-03-16 2023-06-20 中国科学院长春应用化学研究所 一种基于即时检测试纸条的核酸检测方法、检测探针及其试剂盒

Also Published As

Publication number Publication date
US10947534B2 (en) 2021-03-16
JP2022524037A (ja) 2022-04-27
ZA202106516B (en) 2023-10-25
CN113795587A (zh) 2021-12-14
BR112021017655A2 (pt) 2021-11-16
US20200283769A1 (en) 2020-09-10
WO2020181264A1 (en) 2020-09-10
US12331292B2 (en) 2025-06-17
EP3935179A1 (en) 2022-01-12
AU2020232850B2 (en) 2026-03-05
IL286089A (en) 2021-10-31
US20200325474A1 (en) 2020-10-15
JP7733576B2 (ja) 2025-09-03
SG11202109550TA (en) 2021-09-29
US20260109980A1 (en) 2026-04-23
AU2020232850A1 (en) 2021-10-07
EP3935179A4 (en) 2022-11-23
KR20210149060A (ko) 2021-12-08

Similar Documents

Publication Publication Date Title
US10947534B2 (en) RNA-guided DNA integration using Tn7-like transposons
JP6964621B2 (ja) Ｔａｌエフェクターに媒介されるｄｎａ修飾
JP6886433B2 (ja) 植物のゲノム内に外因性配列を組み込むための方法および組成物
WO2018191715A2 (en) Polypeptides with type v crispr activity and uses thereof
CN116555254A (zh) 新颖的rna导向性核酸酶及其用途
US9688997B2 (en) Genetically modified plants with resistance to Xanthomonas and other bacterial plant pathogens
WO2023064895A1 (en) Rna-guided trans-splicing of rna
US20210130833A1 (en) Bacterial defense systems and methods of identifying thereof
US12331291B2 (en) Split complementary base editing systems based on bimolecular deaminases and uses thereof
JP2025529915A (ja) Ｒｎａ誘導核酸媒介遺伝子編集のためのロックド核酸によるガイドｒｎａの化学修飾
US20250215486A1 (en) Liver protective marc variants and uses thereof
US20250154477A1 (en) Nudix overexpressing engineered plants and uses thereof
HK1177626A (en) Tal effector-mediated dna modification

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20240228

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT

Effective date: 20250902

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 5TH ANNIV.) - STANDARD

Year of fee payment: 5

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250903

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250903

D15 Examination report completed

Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT

Effective date: 20251029

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT

Effective date: 20251217

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT RECEIVED - RESPONSE TO EXAMINER'S REQUISITION

Effective date: 20260223

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 6TH ANNIV.) - STANDARD

Year of fee payment: 6

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20260227

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20260227

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT DETERMINED COMPLIANT

Effective date: 20260309

P13 Application amended

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION AMENDED

Effective date: 20260309

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT

Effective date: 20260309