CA2911308A1 - Dispositif pour la preparation et l'analyse d'acides nucleiques - Google Patents
Dispositif pour la preparation et l'analyse d'acides nucleiquesInfo
- Publication number
- CA2911308A1 CA2911308A1 CA2911308A CA2911308A CA2911308A1 CA 2911308 A1 CA2911308 A1 CA 2911308A1 CA 2911308 A CA2911308 A CA 2911308A CA 2911308 A CA2911308 A CA 2911308A CA 2911308 A1 CA2911308 A1 CA 2911308A1
- Authority
- CA
- Canada
- Prior art keywords
- sample
- microfluidic device
- nucleic acid
- microfluidic
- clay mineral
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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| PCT/US2014/037197 WO2014182847A1 (fr) | 2013-05-07 | 2014-05-07 | Dispositif pour la préparation et l'analyse d'acides nucléiques |
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Families Citing this family (29)
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| US9132423B2 (en) | 2010-01-29 | 2015-09-15 | Micronics, Inc. | Sample-to-answer microfluidic cartridge |
| CN104583757A (zh) | 2012-06-28 | 2015-04-29 | 弗洛雷森特里克公司 | 化学指示剂装置 |
| US10518262B2 (en) | 2012-12-21 | 2019-12-31 | Perkinelmer Health Sciences, Inc. | Low elasticity films for microfluidic use |
| JP2016509206A (ja) | 2012-12-21 | 2016-03-24 | マイクロニクス， インコーポレイテッド | 携帯型蛍光検出システムおよびマイクロアッセイカートリッジ |
| KR102102123B1 (ko) | 2012-12-21 | 2020-04-20 | 퍼킨엘머 헬스 사이언시즈, 아이엔씨. | 유체 공학 회로 및 관련 제조 방법 |
| WO2014182844A1 (fr) | 2013-05-07 | 2014-11-13 | Micronics, Inc. | Dispositifs microfluidiques et méthodes de séparation du sérum et de compatibilité croisée du sang |
| US10190153B2 (en) | 2013-05-07 | 2019-01-29 | Micronics, Inc. | Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions |
| JP6484222B2 (ja) | 2013-05-07 | 2019-03-13 | マイクロニクス， インコーポレイテッド | 核酸の調製および分析のためのデバイス |
| EP3281009A4 (fr) | 2015-04-09 | 2018-11-14 | Axela Inc. | Cartouche de test biologique jetable et procédé de réalisation de plusieurs étapes de test et d'un transfert de fluide à l'intérieur de la cartouche |
| JPWO2017043649A1 (ja) * | 2015-09-10 | 2018-07-19 | 株式会社カネカ | 核酸を含有する検体から核酸を分離する方法及びそのための装置 |
| EP3402595A4 (fr) * | 2016-01-11 | 2019-10-02 | Fluoresentric, Inc. | Systèmes, appareil et procédés de préparation d'échantillons en continu |
| AU2017228401B2 (en) * | 2016-03-04 | 2023-07-27 | Abbott Diagnostics Scarborough, Inc. | Automated nested recombinase polymerase amplification |
| CN109416365A (zh) * | 2016-04-13 | 2019-03-01 | 深圳源光科技有限公司 | 用于生物样品筛选的多功能微流体设备 |
| EP3442706A4 (fr) * | 2016-04-13 | 2020-02-19 | NextGen Jane, Inc. | Dispositifs, systèmes et procédés de collecte et de conservation d'échantillon |
| US10427152B2 (en) * | 2016-07-12 | 2019-10-01 | Wi, Inc. | Disposable diagnostic device with vented priming fluid passage for volumetric control of sample and reagents and method of performing a diagnosis therewith |
| GB201704761D0 (en) * | 2017-01-05 | 2017-05-10 | Illumina Inc | Common line selector valve for a system |
| WO2018185143A1 (fr) | 2017-04-04 | 2018-10-11 | Selfdiagnostics Deutschland Gmbh | Distribution de composants de lampe dans une cellule à microfluide |
| JP2021513359A (ja) * | 2018-01-08 | 2021-05-27 | クレイドル ジェノミクス インコーポレイテッド | 核酸単離のための方法およびキット |
| US10935149B2 (en) | 2018-03-15 | 2021-03-02 | University Of Washington | Temperature-actuated valve, fluidic device, and related methods of use |
| CN110433876B (zh) * | 2018-05-03 | 2022-05-17 | 香港科技大学 | 微流控装置及其制造方法、口罩和过滤悬浮颗粒的方法 |
| US20210263027A1 (en) * | 2018-06-22 | 2021-08-26 | Trustees Of Tufts College | Patterned dried blood spot cards and related articles and methods |
| EP3820610A1 (fr) * | 2018-07-12 | 2021-05-19 | Luminex Corporation | Systèmes et procédés permettant d'effectuer des processus de préparation et d'analyse d'échantillons variables |
| GB201819417D0 (en) * | 2018-11-29 | 2019-01-16 | Quantumdx Group Ltd | Vacuum-assisted drying of filters in microfluidic systems |
| IT201900008334A1 (it) | 2019-06-07 | 2020-12-07 | Nurex Srl | Dispositivo automatizzato per l'estrazione, purificazione e concentrazione degli acidi nucleici finalizzato a migliorare la sensibilità e la automazione della rilevazione di cellule nei campioni biologici |
| KR102193197B1 (ko) * | 2019-09-30 | 2020-12-18 | 아주대학교산학협력단 | 소변을 이용한 자동 핵산 검출장치 |
| EP4060017B1 (fr) * | 2019-11-13 | 2024-01-17 | BOE Technology Group Co., Ltd. | Puce de dosage |
| CN111205966B (zh) | 2020-04-18 | 2020-07-21 | 博奥生物集团有限公司 | 样本提取芯片和生物反应装置 |
| CN115537317A (zh) * | 2021-06-30 | 2022-12-30 | 恒泰医疗有限公司 | 检测核酸的芯片装置和仪器及其应用 |
| CN114469897B (zh) * | 2022-01-12 | 2023-02-28 | 上海市胸科医院 | 靶向治疗心肌梗死的锂皂石复合递送材料及其制备与应用 |
Family Cites Families (360)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5622871A (en) | 1987-04-27 | 1997-04-22 | Unilever Patent Holdings B.V. | Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents |
| US3013467A (en) | 1957-11-07 | 1961-12-19 | Minsky Marvin | Microscopy apparatus |
| US3799742A (en) | 1971-12-20 | 1974-03-26 | C Coleman | Miniaturized integrated analytical test container |
| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4181650A (en) | 1975-08-25 | 1980-01-01 | Maier Charles L Jr | Procedure for the assay of pharmacologically immunologically and biochemically active compounds in biological fluids |
| SE399768B (sv) | 1975-09-29 | 1978-02-27 | Lilja Jan E | Kyvett for provtagning, blandning av, provet med ett reagensmedel och direkt utforande av, serskilt optisk, analys av det med reagensmedlet blandade provet |
| US4235960A (en) | 1977-07-29 | 1980-11-25 | The Medical College Of Wisconsin, Inc. | Competitive enzyme-linked immunoassay |
| NL8000173A (nl) | 1980-01-11 | 1981-08-03 | Akzo Nv | Toepassing van in water dispergeerbare, hydrofobe kleurstoffen als label in immunochemische testen. |
| US4304257A (en) | 1980-07-01 | 1981-12-08 | Instrumentation Laboratory Inc. | Valve with flexible sheet member |
| US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
| US4610678A (en) | 1983-06-24 | 1986-09-09 | Weisman Paul T | High-density absorbent structures |
| JPS61137066A (ja) | 1984-12-07 | 1986-06-24 | Toshiba Corp | 自動化学分析装置の測光方式 |
| US4883750A (en) | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US5038852A (en) | 1986-02-25 | 1991-08-13 | Cetus Corporation | Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4788729A (en) | 1985-04-14 | 1988-12-06 | Walker Robert A | Air mattress with audible pressure relief valve |
| WO1986006488A1 (fr) | 1985-04-29 | 1986-11-06 | Hichem Diagnostics, Inc., Dba Bural Technologies | Kit pour tests diagnostiques |
| CA1291031C (fr) | 1985-12-23 | 1991-10-22 | Nikolaas C.J. De Jaeger | Methode pour la detection de liants specifiques et des substances liables par ceux-ci |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US5160701A (en) | 1986-02-18 | 1992-11-03 | Abbott Laboratories | Solid-phase analytical device and method for using same |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| AU622104B2 (en) | 1987-03-11 | 1992-04-02 | Sangtec Molecular Diagnostics Ab | Method of assaying of nucleic acids, a reagent combination and kit therefore |
| CA1303983C (fr) | 1987-03-27 | 1992-06-23 | Robert W. Rosenstein | Essai en phase solide |
| US4810630A (en) | 1987-03-30 | 1989-03-07 | E. I. Du Pont De Nemours And Company | Use of polyoxyethylene ethers to improve the performance of immunoassays employing peroxidase conjugates |
| US4849340A (en) | 1987-04-03 | 1989-07-18 | Cardiovascular Diagnostics, Inc. | Reaction system element and method for performing prothrombin time assay |
| ES2050704T5 (es) | 1987-04-27 | 2004-04-16 | Inverness Medical Switzerland Gmbh | Inmunoensayos y dispositivos para su realizacion. |
| US4855240A (en) | 1987-05-13 | 1989-08-08 | Becton Dickinson And Company | Solid phase assay employing capillary flow |
| US4943522A (en) | 1987-06-01 | 1990-07-24 | Quidel | Lateral flow, non-bibulous membrane assay protocols |
| US4833332A (en) | 1987-06-12 | 1989-05-23 | E. I. Du Pont De Nemours And Company | Scanning fluorescent detection system |
| IL86724A (en) | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
| US5145578A (en) | 1987-07-03 | 1992-09-08 | Shiseido Company Ltd. | Packing material for liquid chromatography |
| US5120643A (en) | 1987-07-13 | 1992-06-09 | Abbott Laboratories | Process for immunochromatography with colloidal particles |
| US4956302A (en) | 1987-09-11 | 1990-09-11 | Abbott Laboratories | Lateral flow chromatographic binding assay device |
| US5275785A (en) | 1987-10-30 | 1994-01-04 | Unilever Patent Holdings B.V. | Test device for detecting an analyte in a liquid sample |
| US4848722A (en) | 1987-12-11 | 1989-07-18 | Integrated Fluidics, Inc. | Valve with flexible sheet member |
| AU622426B2 (en) | 1987-12-11 | 1992-04-09 | Abbott Laboratories | Assay using template-dependent nucleic acid probe reorganization |
| ATE92538T1 (de) | 1988-01-21 | 1993-08-15 | Genentech Inc | Verstaerkung und nachweis von nukleinsaeuresequenzen. |
| US5670381A (en) | 1988-01-29 | 1997-09-23 | Abbott Laboratories | Devices for performing ion-capture binding assays |
| CA1340807C (fr) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Procede d'amplification d'une sequence d'acide nucleique |
| EP0365627B1 (fr) | 1988-03-24 | 1993-12-22 | University Of Iowa Research Foundation | Systemes d'hybridation catalytiques servant a detecter des sequences d'acide nucleique, fondes sur l'activite de ces sequences en tant que cofacteurs dans des reactions catalytiques ou une sonde d'acide nucleique complementaire marquee est soumise a une operation de clivage |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| US5252459A (en) | 1988-09-23 | 1993-10-12 | Abbott Laboratories | Indicator reagents, diagnostic assays and test kits employing organic polymer latex particles |
| CA1339731C (fr) | 1988-10-12 | 1998-03-17 | Charles T. Caskey | Amplification multiplex de l'dn genomique pour la detection de la detection de la deletion |
| US4869282A (en) | 1988-12-09 | 1989-09-26 | Rosemount Inc. | Micromachined valve with polyimide film diaphragm |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| US5075212A (en) | 1989-03-27 | 1991-12-24 | University Of Patents, Inc. | Methods of detecting picornaviruses in biological fluids and tissues |
| FR2647215B1 (fr) | 1989-05-22 | 1991-08-30 | Clextral | Perfectionnement a un appareil de mesure de la contrainte de cisaillement d'une matiere traitee dans une extrudeuse |
| US5225163A (en) | 1989-08-18 | 1993-07-06 | Angenics, Inc. | Reaction apparatus employing gravitational flow |
| JP2520468Y2 (ja) | 1989-08-23 | 1996-12-18 | テイケイ気化器株式会社 | ダイヤフラムポンプ |
| US5075078A (en) | 1989-10-05 | 1991-12-24 | Abbott Laboratories | Self-performing immunochromatographic device |
| US5427930A (en) | 1990-01-26 | 1995-06-27 | Abbott Laboratories | Amplification of target nucleic acids using gap filling ligase chain reaction |
| US5141850A (en) | 1990-02-07 | 1992-08-25 | Hygeia Sciences, Inc. | Porous strip form assay device method |
| US5096837A (en) | 1990-02-08 | 1992-03-17 | Pacific Biotech, Inc. | Immunochromatographic assay and method of using same |
| US5100626A (en) | 1990-05-24 | 1992-03-31 | Levin Andrew E | Binding assay device with removable cassette and manifold |
| US6787338B2 (en) | 1990-06-04 | 2004-09-07 | The University Of Utah | Method for rapid thermal cycling of biological samples |
| GB9014263D0 (en) | 1990-06-27 | 1990-08-15 | Dixon Arthur E | Apparatus and method for spatially- and spectrally- resolvedmeasurements |
| DE69229334T2 (de) | 1991-01-11 | 1999-12-16 | Quidel Corp | Einstufiges querfluss analysenverfahren und ein darin verwendeter nichtsaugfähiger träger |
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| SE9100392D0 (sv) | 1991-02-08 | 1991-02-08 | Pharmacia Biosensor Ab | A method of producing a sealing means in a microfluidic structure and a microfluidic structure comprising such sealing means |
| US5270183A (en) | 1991-02-08 | 1993-12-14 | Beckman Research Institute Of The City Of Hope | Device and method for the automated cycling of solutions between two or more temperatures |
| EP0517631A1 (fr) | 1991-06-07 | 1992-12-09 | Transfluid Sa | Vanne à membrane métallique |
| US5314804A (en) | 1992-03-24 | 1994-05-24 | Serim Research Corporation | Test for Helicobacter pylori |
| US5296703A (en) | 1992-04-01 | 1994-03-22 | The Regents Of The University Of California | Scanning confocal microscope using fluorescence detection |
| US5304487A (en) | 1992-05-01 | 1994-04-19 | Trustees Of The University Of Pennsylvania | Fluid handling in mesoscale analytical devices |
| US6953676B1 (en) | 1992-05-01 | 2005-10-11 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
| US5726026A (en) | 1992-05-01 | 1998-03-10 | Trustees Of The University Of Pennsylvania | Mesoscale sample preparation device and systems for determination and processing of analytes |
| US5587128A (en) | 1992-05-01 | 1996-12-24 | The Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification devices |
| US5498392A (en) | 1992-05-01 | 1996-03-12 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
| US5486335A (en) | 1992-05-01 | 1996-01-23 | Trustees Of The University Of Pennsylvania | Analysis based on flow restriction |
| US6905882B2 (en) | 1992-05-21 | 2005-06-14 | Biosite, Inc. | Diagnostic devices and apparatus for the controlled movement of reagents without membranes |
| WO1994003624A1 (fr) | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Procedes d'amplification isothermique de molecules d'acide nucleique |
| US6472161B1 (en) * | 1992-10-15 | 2002-10-29 | Robert F. Baugh | Method of evaluating blood clot lysis condition |
| JP3563140B2 (ja) | 1995-01-19 | 2004-09-08 | 株式会社日立製作所 | キャピラリーアレイ電気泳動装置 |
| US5730850A (en) | 1993-04-23 | 1998-03-24 | Hitachi, Ltd. | Capillary array electrophoresis system |
| JPH0735736A (ja) | 1993-07-19 | 1995-02-07 | Snow Brand Milk Prod Co Ltd | 液体クロマトグラフィー用分離剤並びにそれを使用する分離及び分析方法 |
| US5415994A (en) | 1993-08-02 | 1995-05-16 | Quidel Corporation | Lateral flow medical diagnostic assay device with sample extraction means |
| US5635602A (en) | 1993-08-13 | 1997-06-03 | The Regents Of The University Of California | Design and synthesis of bispecific DNA-antibody conjugates |
| DE4332720C2 (de) | 1993-09-25 | 1997-02-13 | Karlsruhe Forschzent | Mikromembranpumpe |
| US5447440A (en) * | 1993-10-28 | 1995-09-05 | I-Stat Corporation | Apparatus for assaying viscosity changes in fluid samples and method of conducting same |
| US6309601B1 (en) | 1993-11-01 | 2001-10-30 | Nanogen, Inc. | Scanning optical detection system |
| DK0653639T3 (da) | 1993-11-12 | 2000-07-24 | Unilever Nv | Analyseindretninger og fremgangsmåder til anvendelse deraf |
| JPH07151101A (ja) | 1993-11-29 | 1995-06-13 | Kazuo Sugimura | ダイヤフラム接触面が渦巻状の容器 |
| CH689836A5 (fr) | 1994-01-14 | 1999-12-15 | Westonbridge Int Ltd | Micropompe. |
| US5543026A (en) | 1994-02-07 | 1996-08-06 | The Perkin-Elmer Corporation | Real-time scanning fluorescence electrophoresis apparatus for the analysis of polynucleotide fragments |
| US5631734A (en) | 1994-02-10 | 1997-05-20 | Affymetrix, Inc. | Method and apparatus for detection of fluorescently labeled materials |
| US6287850B1 (en) | 1995-06-07 | 2001-09-11 | Affymetrix, Inc. | Bioarray chip reaction apparatus and its manufacture |
| US5639428A (en) | 1994-07-19 | 1997-06-17 | Becton Dickinson And Company | Method and apparatus for fully automated nucleic acid amplification, nucleic acid assay and immunoassay |
| US5593824A (en) | 1994-09-02 | 1997-01-14 | Pharmacia Biotech, Inc. | Biological reagent spheres |
| GB9419267D0 (en) | 1994-09-23 | 1994-11-09 | Unilever Plc | Assay devices |
| DE4435107C1 (de) | 1994-09-30 | 1996-04-04 | Biometra Biomedizinische Analy | Miniaturisierter Fluß-Thermocycler |
| EP0735144B1 (fr) | 1995-03-28 | 2002-06-05 | Japan Science and Technology Corporation | Procédé pour l'indexation moléculaire de gènes utilisant des enzymes de restriction |
| US5712172A (en) | 1995-05-18 | 1998-01-27 | Wyntek Diagnostics, Inc. | One step immunochromatographic device and method of use |
| SE504193C2 (sv) | 1995-04-21 | 1996-12-02 | Hemocue Ab | Kapillär mikrokyvett |
| US5578818A (en) | 1995-05-10 | 1996-11-26 | Molecular Dynamics | LED point scanning system |
| CA2222126A1 (fr) | 1995-06-16 | 1997-01-03 | Fred K. Forster | Procede et dispositif miniaturise d'extraction differentielle |
| WO1997000121A1 (fr) | 1995-06-16 | 1997-01-03 | The University Of Washington | Filtre pour fluides micro-usine, plan et a debit tangentiel |
| US5716852A (en) | 1996-03-29 | 1998-02-10 | University Of Washington | Microfabricated diffusion-based chemical sensor |
| SE9502258D0 (sv) | 1995-06-21 | 1995-06-21 | Pharmacia Biotech Ab | Method for the manufacture of a membrane-containing microstructure |
| US5856174A (en) | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| US6168948B1 (en) | 1995-06-29 | 2001-01-02 | Affymetrix, Inc. | Miniaturized genetic analysis systems and methods |
| US5724404A (en) | 1995-07-03 | 1998-03-03 | Garcia; Max | Integrated international telephone circuit monitoring system |
| US5989813A (en) | 1995-07-13 | 1999-11-23 | Molecular Innovations, Inc. | Detection of amplified nucleic acid sequences using bifunctional haptenization and dyed microparticles |
| US5660990A (en) | 1995-08-18 | 1997-08-26 | Immunivest Corporation | Surface immobilization of magnetically collected materials |
| US5726751A (en) | 1995-09-27 | 1998-03-10 | University Of Washington | Silicon microchannel optical flow cytometer |
| US5705018A (en) | 1995-12-13 | 1998-01-06 | Hartley; Frank T. | Micromachined peristaltic pump |
| US5863502A (en) | 1996-01-24 | 1999-01-26 | Sarnoff Corporation | Parallel reaction cassette and associated devices |
| US5741647A (en) | 1996-02-16 | 1998-04-21 | Tam; Joseph Wing On | Flow through nucleic acid hybridisation uses thereof and a device thereof |
| US5660370A (en) | 1996-03-07 | 1997-08-26 | Integrated Fludics, Inc. | Valve with flexible sheet member and two port non-flexing backer member |
| US5972721A (en) | 1996-03-14 | 1999-10-26 | The United States Of America As Represented By The Secretary Of The Air Force | Immunomagnetic assay system for clinical diagnosis and other purposes |
| US5747349A (en) | 1996-03-20 | 1998-05-05 | University Of Washington | Fluorescent reporter beads for fluid analysis |
| US5948684A (en) | 1997-03-31 | 1999-09-07 | University Of Washington | Simultaneous analyte determination and reference balancing in reference T-sensor devices |
| US6541213B1 (en) | 1996-03-29 | 2003-04-01 | University Of Washington | Microscale diffusion immunoassay |
| US6825047B1 (en) | 1996-04-03 | 2004-11-30 | Applera Corporation | Device and method for multiple analyte detection |
| US6001307A (en) | 1996-04-26 | 1999-12-14 | Kyoto Daiichi Kagaku Co., Ltd. | Device for analyzing a sample |
| US6057167A (en) | 1996-05-31 | 2000-05-02 | Motorola, Inc. | Magnetoresistance-based method and apparatus for molecular detection |
| US5726404A (en) | 1996-05-31 | 1998-03-10 | University Of Washington | Valveless liquid microswitch |
| US5863801A (en) | 1996-06-14 | 1999-01-26 | Sarnoff Corporation | Automated nucleic acid isolation |
| DE69728269T2 (de) | 1996-06-14 | 2005-03-10 | University Of Washington, Seattle | Absorbtionsverbessertes differentielles extraktionsverfahren |
| US6582921B2 (en) | 1996-07-29 | 2003-06-24 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses thereof |
| JP2001502791A (ja) | 1996-08-12 | 2001-02-27 | ハンプシャー アドバイザリー アンド テクニカル サービシーズ リミテッド | 診断用テスト容器 |
| JPH1082773A (ja) | 1996-09-10 | 1998-03-31 | Kdk Corp | クロマトグラフィ装置における溶離液の供給方法、および溶離液収容体 |
| US5798273A (en) | 1996-09-25 | 1998-08-25 | Becton Dickinson And Company | Direct read lateral flow assay for small analytes |
| US5748827A (en) | 1996-10-23 | 1998-05-05 | University Of Washington | Two-stage kinematic mount |
| US5971355A (en) | 1996-11-27 | 1999-10-26 | Xerox Corporation | Microdevice valve structures to fluid control |
| JP3372436B2 (ja) | 1996-11-28 | 2003-02-04 | オークマ株式会社 | インバータの制御装置 |
| US7052594B2 (en) | 2002-01-31 | 2006-05-30 | Sri International | Devices and methods for controlling fluid flow using elastic sheet deflection |
| US5906602A (en) | 1997-03-27 | 1999-05-25 | The Procter & Gamble Company | Shaped absorbent cores comprising multiple pieces of absorbent material and method for making same |
| WO1998049548A1 (fr) | 1997-04-25 | 1998-11-05 | Caliper Technologies Corporation | Dispositifs microfluidiques a geometrie de canaux amelioree |
| US5974867A (en) | 1997-06-13 | 1999-11-02 | University Of Washington | Method for determining concentration of a laminar sample stream |
| EP0991928A2 (fr) | 1997-06-27 | 2000-04-12 | Immunetics | Appareil et procede d'essai de liaison rapide en ecoulement continu |
| US20050037397A1 (en) | 2001-03-28 | 2005-02-17 | Nanosphere, Inc. | Bio-barcode based detection of target analytes |
| US6974669B2 (en) | 2000-03-28 | 2005-12-13 | Nanosphere, Inc. | Bio-barcodes based on oligonucleotide-modified nanoparticles |
| US5932799A (en) | 1997-07-21 | 1999-08-03 | Ysi Incorporated | Microfluidic analyzer module |
| US6390791B1 (en) | 1997-08-20 | 2002-05-21 | Westonbridge International Limited | Micro pump comprising an inlet control member for its self-priming |
| JP3498201B2 (ja) | 1997-08-27 | 2004-02-16 | アークレイ株式会社 | 引圧発生装置およびそれを用いた検体分析装置 |
| US5965410A (en) | 1997-09-02 | 1999-10-12 | Caliper Technologies Corp. | Electrical current for controlling fluid parameters in microchannels |
| US6007775A (en) | 1997-09-26 | 1999-12-28 | University Of Washington | Multiple analyte diffusion based chemical sensor |
| US6953675B2 (en) | 1997-11-06 | 2005-10-11 | Immunomedics, Inc. | Landscaped antibodies and antibody fragments for clinical use |
| US6037168A (en) | 1997-12-31 | 2000-03-14 | Cytonix Corporation | Microbiological assembly comprising resealable closure means |
| SE9800070D0 (sv) | 1998-01-14 | 1998-01-14 | Hemocue Ab | Blandningsmetod |
| US6210882B1 (en) | 1998-01-29 | 2001-04-03 | Mayo Foundation For Medical Education And Reseach | Rapid thermocycling for sample analysis |
| US6210514B1 (en) | 1998-02-11 | 2001-04-03 | Xerox Corporation | Thin film structure machining and attachment |
| US6018616A (en) | 1998-02-23 | 2000-01-25 | Applied Materials, Inc. | Thermal cycling module and process using radiant heat |
| AU3771599A (en) | 1998-05-18 | 1999-12-06 | University Of Washington | Liquid analysis cartridge |
| US6637463B1 (en) | 1998-10-13 | 2003-10-28 | Biomicro Systems, Inc. | Multi-channel microfluidic system design with balanced fluid flow distribution |
| US6158712A (en) | 1998-10-16 | 2000-12-12 | Agilent Technologies, Inc. | Multilayer integrated assembly having an integral microminiature valve |
| US6086740A (en) | 1998-10-29 | 2000-07-11 | Caliper Technologies Corp. | Multiplexed microfluidic devices and systems |
| US6887693B2 (en) | 1998-12-24 | 2005-05-03 | Cepheid | Device and method for lysing cells, spores, or microorganisms |
| ATE556149T1 (de) | 1999-02-23 | 2012-05-15 | Caliper Life Sciences Inc | Manipulation von mikropartikeln in mikrofluidischen systemen |
| US6171850B1 (en) | 1999-03-08 | 2001-01-09 | Caliper Technologies Corp. | Integrated devices and systems for performing temperature controlled reactions and analyses |
| US6673620B1 (en) | 1999-04-20 | 2004-01-06 | Cytologix Corporation | Fluid exchange in a chamber on a microscope slide |
| DE60040977D1 (de) | 1999-04-20 | 2009-01-15 | Cytologix Corp | Flüssigkeitswechsel in einer kammer auf einem mikroskopobjektträger |
| JP4085514B2 (ja) | 1999-04-30 | 2008-05-14 | 株式会社島津製作所 | 電気泳動チップ |
| US6399952B1 (en) | 1999-05-12 | 2002-06-04 | Aclara Biosciences, Inc. | Multiplexed fluorescent detection in microfluidic devices |
| ATE278771T1 (de) | 1999-05-28 | 2004-10-15 | Cepheid | Vorrichtung und verfahren zur analyse flüssiger proben |
| US6899137B2 (en) | 1999-06-28 | 2005-05-31 | California Institute Of Technology | Microfabricated elastomeric valve and pump systems |
| MXPA01012959A (es) | 1999-06-28 | 2002-07-30 | California Inst Of Techn | Sistemas elastomericos, microfabricados, de valvulas y bombas. |
| US6815160B1 (en) | 1999-07-28 | 2004-11-09 | Chiron Corporation | Hepatitis C viral antigen immunoassay detection systems |
| US6731781B1 (en) | 1999-09-30 | 2004-05-04 | Biodiscovery, Inc. | System and method for automatically processing microarrays |
| WO2001026813A2 (fr) | 1999-10-08 | 2001-04-19 | Micronics, Inc. | Logique a microfluides sans pompe |
| US6272939B1 (en) | 1999-10-15 | 2001-08-14 | Applera Corporation | System and method for filling a substrate with a liquid sample |
| US6569674B1 (en) | 1999-12-15 | 2003-05-27 | Amersham Biosciences Ab | Method and apparatus for performing biological reactions on a substrate surface |
| DE60131927D1 (de) | 2000-02-02 | 2008-01-31 | Univ Florida State Res Found | Heterosubstituierte taxan-c10-acetate als antitumormittel |
| US20040121364A1 (en) | 2000-02-07 | 2004-06-24 | Mark Chee | Multiplex nucleic acid reactions |
| US6309875B1 (en) | 2000-02-29 | 2001-10-30 | Agilent Technologies, Inc. | Apparatus for biomolecular array hybridization facilitated by agitation during centrifuging |
| JP4733331B2 (ja) | 2000-03-14 | 2011-07-27 | マイクロニックス、インコーポレーテッド | マイクロ流動体分析用デバイス |
| CA2402985A1 (fr) | 2000-03-21 | 2001-09-27 | Brevard S. Garrison | Procede et dispositif de regulation acoustique de solutions liquides dans des dispositifs microfluidiques |
| US6635487B1 (en) | 2000-05-17 | 2003-10-21 | Caliper Technologies Corp. | Fluorescence standard for use in microfluidic instruments |
| AU7125401A (en) | 2000-05-19 | 2001-12-03 | David J Marshall | Materials and methods for detection of nucleic acids |
| US6431212B1 (en) | 2000-05-24 | 2002-08-13 | Jon W. Hayenga | Valve for use in microfluidic structures |
| WO2001089675A2 (fr) | 2000-05-24 | 2001-11-29 | Micronics, Inc. | Melangeur a vortex et a jet |
| US7553453B2 (en) | 2000-06-02 | 2009-06-30 | Honeywell International Inc. | Assay implementation in a microfluidic format |
| US6568286B1 (en) | 2000-06-02 | 2003-05-27 | Honeywell International Inc. | 3D array of integrated cells for the sampling and detection of air bound chemical and biological species |
| US7351376B1 (en) | 2000-06-05 | 2008-04-01 | California Institute Of Technology | Integrated active flux microfluidic devices and methods |
| US7087414B2 (en) | 2000-06-06 | 2006-08-08 | Applera Corporation | Methods and devices for multiplexing amplification reactions |
| US6439036B1 (en) | 2000-06-13 | 2002-08-27 | Symyx Technologics, Inc. | Method for evaluating a test fluid |
| US6581899B2 (en) | 2000-06-23 | 2003-06-24 | Micronics, Inc. | Valve for use in microfluidic structures |
| CA2417341A1 (fr) | 2000-08-08 | 2002-02-14 | Jing Cheng | Techniques de manipulations de fragments dans des systemes microfluidiques |
| AU2001290867A1 (en) | 2000-09-14 | 2002-03-26 | Caliper Technologies Corp. | Microfluidic devices and methods for performing temperature mediated reactions |
| AU2002211389A1 (en) | 2000-10-03 | 2002-04-15 | California Institute Of Technology | Microfluidic devices and methods of use |
| AU9657901A (en) | 2000-10-03 | 2002-04-15 | Minerva Biotechnologies Corp | Magnetic in situ dilution |
| WO2002061387A2 (fr) | 2000-10-25 | 2002-08-08 | Exiqon A/S | Plates-formes du type substrat ouvert se pretant a l'analyse de biomolecules |
| FR2816074B1 (fr) | 2000-10-30 | 2003-01-03 | St Microelectronics Sa | Generateur digital precis produisant des signaux d'horloge |
| AU2002248149A1 (en) | 2000-11-16 | 2002-08-12 | Fluidigm Corporation | Microfluidic devices for introducing and dispensing fluids from microfluidic systems |
| GB0028647D0 (en) | 2000-11-24 | 2001-01-10 | Nextgen Sciences Ltd | Apparatus for chemical assays |
| JP2002190257A (ja) | 2000-12-22 | 2002-07-05 | Sanyo Electric Co Ltd | マグネトロンの製法 |
| DE60015590T2 (de) | 2000-12-28 | 2005-11-10 | Roche Diagnostics Gmbh | Verfahren zur Behandlung von Nukleinsaüreproben mittels Schwingung eines Teiles einer Kartuschenwandung, System und Kartusche zur Durchführung desselben |
| US6767194B2 (en) | 2001-01-08 | 2004-07-27 | President And Fellows Of Harvard College | Valves and pumps for microfluidic systems and method for making microfluidic systems |
| DE10111457B4 (de) | 2001-03-09 | 2006-12-14 | Siemens Ag | Diagnoseeinrichtung |
| WO2003015923A1 (fr) | 2001-08-20 | 2003-02-27 | Biomicro Systems, Inc. | Melange de fluides dans des chambres a faible rapport de forme |
| KR100916074B1 (ko) | 2001-03-09 | 2009-09-08 | 바이오마이크로 시스템즈, 인크. | 어레이에 대한 마이크로유체 인터페이스 방법 및인터페이스용 시스템 |
| US6576459B2 (en) | 2001-03-23 | 2003-06-10 | The Regents Of The University Of California | Sample preparation and detection device for infectious agents |
| US7010391B2 (en) | 2001-03-28 | 2006-03-07 | Handylab, Inc. | Methods and systems for control of microfluidic devices |
| US20020160518A1 (en) | 2001-04-03 | 2002-10-31 | Hayenga Jon W. | Microfluidic sedimentation |
| US6802342B2 (en) | 2001-04-06 | 2004-10-12 | Fluidigm Corporation | Microfabricated fluidic circuit elements and applications |
| EP1384022A4 (fr) | 2001-04-06 | 2004-08-04 | California Inst Of Techn | Amplification d'acide nucleique au moyen de dispositifs microfluidiques |
| US6562209B1 (en) | 2001-04-19 | 2003-05-13 | Northrop Grumman Corporation | Automated computer controlled reporter device for conducting imunnoassay and molecular biology procedures |
| US6418968B1 (en) | 2001-04-20 | 2002-07-16 | Nanostream, Inc. | Porous microfluidic valves |
| DE50212817D1 (de) | 2001-05-25 | 2008-11-06 | Tecan Trading Ag | Vorrichtung und Verfahrenseinheit zum Bereitstellen eines Hybridisierraums |
| US6729352B2 (en) | 2001-06-07 | 2004-05-04 | Nanostream, Inc. | Microfluidic synthesis devices and methods |
| US20030032028A1 (en) | 2001-06-12 | 2003-02-13 | Gayle Dace | In vitro capture of nucleic acids via modified oligonucleotides and magnetic beads |
| US20020192676A1 (en) | 2001-06-18 | 2002-12-19 | Madonna Angelo J. | Method for determining if a type of bacteria is present in a mixture |
| US7141416B2 (en) | 2001-07-12 | 2006-11-28 | Burstein Technologies, Inc. | Multi-purpose optical analysis optical bio-disc for conducting assays and various reporting agents for use therewith |
| US20040226348A1 (en) | 2001-07-24 | 2004-11-18 | Phillip Bruce | Magnetic assisted detection of magnetic beads using optical disc drives |
| WO2003029632A1 (fr) | 2001-09-27 | 2003-04-10 | Robert Bosch Gmbh | Unite de reglage |
| GB0124341D0 (en) | 2001-10-10 | 2001-11-28 | Randox Lab Ltd | Assay |
| US7344894B2 (en) | 2001-10-16 | 2008-03-18 | Agilent Technologies, Inc. | Thermal regulation of fluidic samples within a diagnostic cartridge |
| EP1541602B1 (fr) | 2001-11-01 | 2010-09-29 | Mitsubishi Chemical Corporation | Polymer de propylène modifié, composition contenant celui-ci et son utilisation |
| US6554591B1 (en) | 2001-11-26 | 2003-04-29 | Motorola, Inc. | Micropump including ball check valve utilizing ceramic technology and method of fabrication |
| JP2003166910A (ja) | 2001-11-30 | 2003-06-13 | Asahi Kasei Corp | 送液機構及び該送液機構を備える分析装置 |
| US7691333B2 (en) | 2001-11-30 | 2010-04-06 | Fluidigm Corporation | Microfluidic device and methods of using same |
| GB0129816D0 (en) | 2001-12-13 | 2002-01-30 | The Technology Partnership Plc | Testing device for chemical or biochemical analysis |
| US7048890B2 (en) | 2001-12-21 | 2006-05-23 | Koninklijke Philips Electronics N.V. | Sensor and method for measuring the areal density of magnetic nanoparticles on a micro-array |
| US6748975B2 (en) | 2001-12-26 | 2004-06-15 | Micralyne Inc. | Microfluidic valve and method of manufacturing same |
| US6773677B2 (en) | 2002-01-09 | 2004-08-10 | Caliper Life Sciences, Inc. | Slide cassette for fluidic injection |
| JP2003207454A (ja) | 2002-01-15 | 2003-07-25 | Minolta Co Ltd | 透過光検出装置 |
| US7223371B2 (en) | 2002-03-14 | 2007-05-29 | Micronics, Inc. | Microfluidic channel network device |
| AUPS159702A0 (en) | 2002-04-09 | 2002-05-16 | Tong, Sun Wing | Molecular detection and assay by magneto-thermal biochip micro-assay |
| US6955738B2 (en) | 2002-04-09 | 2005-10-18 | Gyros Ab | Microfluidic devices with new inner surfaces |
| US7785776B2 (en) | 2002-05-13 | 2010-08-31 | Idaho Technology, Inc. | Genotyping by amplicon melting curve analysis |
| DE10222133A1 (de) * | 2002-05-17 | 2003-12-04 | Gl Biotech Gmbh | Verfahren zur Nukleinsäure-Extraktion und Nukleinsäure-Reinigung |
| WO2003097831A1 (fr) | 2002-05-17 | 2003-11-27 | Gl Bio Tech Gmbh | Procede d'extraction et de purification d'acides nucleiques |
| JP4494962B2 (ja) | 2002-05-24 | 2010-06-30 | バクスター・インターナショナル・インコーポレイテッド | 自動化された透析機器のための電気的システム、方法および装置 |
| US20040033627A1 (en) | 2002-05-31 | 2004-02-19 | The Regents Of The University Of California | Method and apparatus for detecting substances of interest |
| CZ297082B6 (cs) | 2002-06-03 | 2006-09-13 | Ing. Ilja Krejcí-Engineering | Soucástky s trojrozmernou strukturou pripravené tlustovrstvou technologií a zpusob jejich výroby |
| JP4106977B2 (ja) | 2002-06-21 | 2008-06-25 | 株式会社日立製作所 | 分析チップ及び分析装置 |
| US6843263B2 (en) | 2002-06-24 | 2005-01-18 | Industrial Technology Research Institute | Partially closed microfluidic system and microfluidic driving method |
| US7442342B2 (en) | 2002-06-26 | 2008-10-28 | Ge Healthcare Bio-Sciences Ab | Biochip holder and method of collecting fluid |
| JP2004037338A (ja) | 2002-07-05 | 2004-02-05 | Yokogawa Electric Corp | 磁気ビーズを用いて生体高分子を基板へ固定する方法およびその方法を用いた生体高分子測定装置 |
| US20040018611A1 (en) | 2002-07-23 | 2004-01-29 | Ward Michael Dennis | Microfluidic devices for high gradient magnetic separation |
| US6817373B2 (en) | 2002-07-26 | 2004-11-16 | Applera Corporation | One-directional microball valve for a microfluidic device |
| US7244961B2 (en) | 2002-08-02 | 2007-07-17 | Silicon Valley Scientific | Integrated system with modular microfluidic components |
| US20040226620A1 (en) * | 2002-09-26 | 2004-11-18 | Daniel Therriault | Microcapillary networks |
| US7604932B2 (en) | 2002-11-13 | 2009-10-20 | Haematologic Technologies, Inc. | Assay for tissue factor in a sample |
| WO2004055199A2 (fr) | 2002-12-12 | 2004-07-01 | Chiron Corporation | Dispositif de stockage d'echantillons biologiques et procede permettant de deceler une eventuelle contamination d'echantillons biologiques |
| US8676383B2 (en) | 2002-12-23 | 2014-03-18 | Applied Biosystems, Llc | Device for carrying out chemical or biological reactions |
| EP2711415B1 (fr) | 2002-12-26 | 2022-02-16 | Meso Scale Technologies, LLC. | Cartouches de test et procédés d'utilisation |
| US6807892B2 (en) | 2002-12-30 | 2004-10-26 | Xerox Corporation | Pneumatic actuator with elastomeric membrane and low-power electrostatic flap valve arrangement |
| US7445926B2 (en) | 2002-12-30 | 2008-11-04 | The Regents Of The University Of California | Fluid control structures in microfluidic devices |
| US20050221281A1 (en) | 2003-01-08 | 2005-10-06 | Ho Winston Z | Self-contained microfluidic biochip and apparatus |
| US7419638B2 (en) | 2003-01-14 | 2008-09-02 | Micronics, Inc. | Microfluidic devices for fluid manipulation and analysis |
| EP1587623A2 (fr) | 2003-01-14 | 2005-10-26 | Micronics, Inc. | Dispositifs microfluidiques pour la manipulation et l'analyse de fluides |
| EP1590482B1 (fr) | 2003-01-17 | 2008-12-10 | Eragen Biosciences, Inc. | Amplification d'acides nucleiques au moyen de bases non standard |
| AU2004205671A1 (en) | 2003-01-21 | 2004-08-05 | Perkinelmer Health Sciences, Inc. | Method and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing |
| US20070125947A1 (en) | 2003-02-20 | 2007-06-07 | David Sprinzak | Sample enclosure for a scanning electron microscope and methods of use thereof |
| EP1601438B1 (fr) | 2003-03-08 | 2009-10-07 | Ecole Polytechnique Federale de Lausanne EPFL-SRI | Dispositif de manipulation et de transport de perles magnetiques |
| EP1473085B1 (fr) | 2003-03-31 | 2015-07-22 | Canon Kabushiki Kaisha | Cassette pour réaction biochimique |
| JP4260541B2 (ja) | 2003-05-12 | 2009-04-30 | 旭化成ファーマ株式会社 | 糖化タンパク質測定用試験片 |
| US6916113B2 (en) | 2003-05-16 | 2005-07-12 | Agilent Technologies, Inc. | Devices and methods for fluid mixing |
| CA2526368A1 (fr) | 2003-05-20 | 2004-12-02 | Fluidigm Corporation | Procede et systeme pour dispositif microfluidique et son imagerie |
| US7648835B2 (en) | 2003-06-06 | 2010-01-19 | Micronics, Inc. | System and method for heating, cooling and heat cycling on microfluidic device |
| CN1812839A (zh) | 2003-06-06 | 2006-08-02 | 精密公司 | 在微流体装置上的加热、冷却和热循环的系统与方法 |
| DE10336849A1 (de) | 2003-08-11 | 2005-03-10 | Thinxxs Gmbh | Flusszelle |
| WO2005045075A2 (fr) | 2003-08-12 | 2005-05-19 | Massachusetts Institute Of Technology | Procedes de preparation d'echantillons et dispositifs associes |
| EP1659405B1 (fr) | 2003-08-29 | 2012-02-29 | Asahi Kasei Kabushiki Kaisha | Biocatpeur et procede de mesure d'une substance a analyser |
| CA2479452C (fr) | 2003-08-30 | 2008-11-04 | F.Hoffmann-La Roche Ag | Methode et dispositif de determination de substances a analyser dans un liquide |
| US7329742B2 (en) | 2003-09-04 | 2008-02-12 | The Regents Of The University Of California | Aptamers and methods for their in vitro selection and uses thereof |
| DE10352716A1 (de) | 2003-11-05 | 2005-06-16 | Einsle, Xaver | Plattform |
| GB0327094D0 (en) | 2003-11-21 | 2003-12-24 | Inverness Medical Switzerland | Laminated device |
| WO2005052578A1 (fr) | 2003-11-28 | 2005-06-09 | Olympus Corporation | Appareil de test de substances biologiques et etage de reaction dudit appareil |
| US7575862B2 (en) | 2003-12-09 | 2009-08-18 | Asiagen Corporation | Assay systems, kits and methods for detecting microorganisms |
| EP1712919B1 (fr) | 2004-01-07 | 2012-08-15 | ARKRAY, Inc. | Instrument analytique a agencement ameliore de section de reactif et procede analytique correspondant |
| JP4417332B2 (ja) | 2004-01-15 | 2010-02-17 | 独立行政法人科学技術振興機構 | 化学分析装置及び化学分析方法 |
| US7221455B2 (en) | 2004-01-20 | 2007-05-22 | The Regents Of The Unversity Of California | Integrated, fluorescence-detecting microanalytical system |
| US20050164373A1 (en) | 2004-01-22 | 2005-07-28 | Oldham Mark F. | Diffusion-aided loading system for microfluidic devices |
| WO2005082043A2 (fr) | 2004-02-24 | 2005-09-09 | Thermal Gradient | Dispositif de cycle thermique |
| JPWO2005088280A1 (ja) | 2004-03-17 | 2008-01-31 | オリンパス株式会社 | 光測定装置及び光測定方法 |
| US7156117B2 (en) | 2004-03-31 | 2007-01-02 | Lifescan Scotland Limited | Method of controlling the movement of fluid through a microfluidic circuit using an array of triggerable passive valves |
| CA2563613A1 (fr) | 2004-04-16 | 2005-12-08 | Wei-Sing Chu | Appareil pour extraire les molecules biologiques des echantillons de tissu |
| DE102004021780B4 (de) | 2004-04-30 | 2008-10-02 | Siemens Ag | Verfahren und Anordnung zur DNA-Isolierung mit Trockenreagenzien |
| JP3952036B2 (ja) | 2004-05-13 | 2007-08-01 | コニカミノルタセンシング株式会社 | マイクロ流体デバイス並びに試液の試験方法および試験システム |
| AU2005323451B2 (en) | 2004-05-21 | 2010-11-18 | Qiagen Sciences Llc | Kits and processes for removing contaminants from nucleic acids in environmental and biological samples |
| FI20040768A0 (fi) | 2004-06-04 | 2004-06-04 | Teemu Korpimaeki | Menetelmä määritysreagenssien stabiloimiseksi, stabilisoituja määritysreagensseja sisältävä reagenssisäiliö ja sen käyttö |
| JP2005345378A (ja) | 2004-06-04 | 2005-12-15 | Nippon Sheet Glass Co Ltd | 光検出装置 |
| CA2569601C (fr) | 2004-06-07 | 2014-10-21 | Iquum, Inc. | Multitraitement d'echantillons |
| US7615370B2 (en) | 2004-07-08 | 2009-11-10 | Tecan Trading Ag | System having device for preventing air bubbles in a hybridization chamber and corresponding method |
| EP1614466A3 (fr) | 2004-07-08 | 2006-05-10 | Tecan Trading AG | Dispositif et procédé pour la prévention de bulles d'air dans une chambre d'hybridation |
| DE202004012163U1 (de) | 2004-07-08 | 2004-10-07 | Tecan Trading Ag | System mit Einrichtung zum Verhindern von Luftblasen in einer Hybridisierkammer |
| WO2006018811A1 (fr) | 2004-08-20 | 2006-02-23 | Instituto De Engenharia De Sistemas E Computadores Para Os Microsistemas E As Nanotecnologias (Inesc-Mn) | Dispositif bioelectronique |
| JP5028736B2 (ja) | 2004-09-02 | 2012-09-19 | 東レ株式会社 | 高分子電解質材、ならびにそれを用いた高分子電解質膜、膜電極複合体および高分子電解質型燃料電池 |
| JP4375183B2 (ja) | 2004-09-22 | 2009-12-02 | ウシオ電機株式会社 | マイクロチップ |
| WO2006036592A1 (fr) | 2004-09-23 | 2006-04-06 | University Of Washington | Immuno-essai de diffusion a micro-echelle utilisant des reactifs polyvalents |
| WO2006035830A1 (fr) | 2004-09-30 | 2006-04-06 | Arkray, Inc. | Analyseur |
| US7832429B2 (en) | 2004-10-13 | 2010-11-16 | Rheonix, Inc. | Microfluidic pump and valve structures and fabrication methods |
| JP2006122743A (ja) | 2004-10-26 | 2006-05-18 | Dainippon Screen Mfg Co Ltd | マイクロリアクタ装置 |
| US20060094028A1 (en) | 2004-11-04 | 2006-05-04 | Welch Allyn, Inc. | Rapid diagnostic assay |
| US20060127886A1 (en) | 2004-12-15 | 2006-06-15 | Kaylor Rosann M | Sample-efficient lateral flow immunoassay |
| WO2006076567A2 (fr) | 2005-01-13 | 2006-07-20 | Micronics, Inc. | Dispositif de detection microfluidique de cellules rares |
| EP1846676A2 (fr) | 2005-01-31 | 2007-10-24 | The President and Fellows of Harvard College | Vannes et reservoirs de systemes microfluidiques |
| JP2006227301A (ja) | 2005-02-17 | 2006-08-31 | Olympus Corp | 蛍光観察装置 |
| JP2006246777A (ja) | 2005-03-10 | 2006-09-21 | Canon Inc | 生化学反応用カートリッジおよび生化学反応カートリッジ内での溶液の移動方法 |
| WO2006116616A2 (fr) | 2005-04-26 | 2006-11-02 | Applera Corporation | Systemes et procedes de detection multiple d'analytes |
| WO2006130299A2 (fr) | 2005-05-03 | 2006-12-07 | Micronics, Inc. | Bande de detection de flux laminaire microfluidique |
| NZ564141A (en) | 2005-05-09 | 2011-02-25 | Theranos Inc | Two way communication system for monitoring an analyte |
| US20060254916A1 (en) * | 2005-05-16 | 2006-11-16 | Hernandez Juan J | Methods of forming microfluidic devices and uses thereof |
| AU2006251637B2 (en) | 2005-05-20 | 2012-06-14 | Integrated Dna Technologies, Inc. | Compounds and methods for labeling oligonucleotides |
| US9110044B2 (en) | 2005-05-25 | 2015-08-18 | Boehringer Ingelheim Vetmedica Gmbh | System for the integrated and automated analysis of DNA or protein and method for operating said type of system |
| WO2006132666A1 (fr) | 2005-06-06 | 2006-12-14 | Decision Biomarkers, Inc. | Epreuves fondees sur des agencements d'ecoulement liquide |
| WO2007002480A2 (fr) | 2005-06-24 | 2007-01-04 | Board Of Regents, The University Of Texas System | Systemes et procedes faisant appel a des cartouches autonomes comprenant des systemes de detection et des systemes de distribution de fluides |
| US7527763B2 (en) | 2005-07-05 | 2009-05-05 | 3M Innovative Properties Company | Valve control system for a rotating multiplex fluorescence detection device |
| JP4692200B2 (ja) | 2005-10-06 | 2011-06-01 | 横河電機株式会社 | 化学処理用カートリッジおよびその使用方法 |
| WO2007049009A1 (fr) | 2005-10-25 | 2007-05-03 | Inverness Medical Switzerland Gmbh | Procédé et équipement pour détecter une substance à analyser dans un échantillon |
| CN101305274A (zh) | 2005-11-07 | 2008-11-12 | 皇家飞利浦电子股份有限公司 | 基于柱的生物传感器及其制造方法 |
| US9056291B2 (en) | 2005-11-30 | 2015-06-16 | Micronics, Inc. | Microfluidic reactor system |
| US7763453B2 (en) | 2005-11-30 | 2010-07-27 | Micronics, Inc. | Microfluidic mixing and analytic apparatus |
| US20070154895A1 (en) | 2005-12-30 | 2007-07-05 | Caliper Life Sciences, Inc. | Multi-assay microfluidic chips |
| US20090061450A1 (en) | 2006-03-14 | 2009-03-05 | Micronics, Inc. | System and method for diagnosis of infectious diseases |
| EP2007905B1 (fr) * | 2006-03-15 | 2012-08-22 | Micronics, Inc. | Dosages d'acides nucléiques intégrés |
| US7718193B2 (en) | 2006-03-16 | 2010-05-18 | University Of Washington | Temperature- and pH-responsive polymer compositions |
| US20070234785A1 (en) | 2006-03-30 | 2007-10-11 | Timothy Beerling | System and method for using a flexible membrane for controlling fluid flow in a microfluidic circuit |
| EP2027251A4 (fr) | 2006-05-17 | 2010-05-05 | California Inst Of Techn | Systeme de cyclage thermique |
| EP2210955A3 (fr) | 2006-05-23 | 2010-09-15 | Molecular Detection, Inc. | Kits de température ambiante stable pour diagnostics moléculaires |
| US20070280856A1 (en) | 2006-06-02 | 2007-12-06 | Applera Corporation | Devices and Methods for Controlling Bubble Formation in Microfluidic Devices |
| EP2041573B1 (fr) | 2006-06-23 | 2019-09-04 | PerkinElmer Health Sciences, Inc. | Procédés et dispositifs destinés à des dosages immunologiques microfluidiques pratiqués au point de service |
| US7674616B2 (en) * | 2006-09-14 | 2010-03-09 | Hemosense, Inc. | Device and method for measuring properties of a sample |
| EP2063866B2 (fr) | 2006-09-18 | 2017-08-23 | GE Healthcare Bio-Sciences Corp. | Préparation de réactifs biologiques vitrifiés |
| WO2008147382A1 (fr) | 2006-09-27 | 2008-12-04 | Micronics, Inc. | Dispositifs d'analyse microfluidique intégrés et procédés |
| US7749775B2 (en) | 2006-10-03 | 2010-07-06 | Jonathan Scott Maher | Immunoassay test device and method of use |
| US7607641B1 (en) | 2006-10-05 | 2009-10-27 | Microfluidic Systems, Inc. | Microfluidic valve mechanism |
| JP4888035B2 (ja) | 2006-10-16 | 2012-02-29 | 凸版印刷株式会社 | 酸素インジケーターおよび包装材料 |
| WO2008061165A2 (fr) | 2006-11-14 | 2008-05-22 | Handylab, Inc. | Cartouche microfluidique et son procédé de fabrication |
| CA2716575C (fr) | 2007-02-23 | 2015-09-29 | Ese Embedded System Engineering Gmbh | Instrument de mesure optique |
| US20080253911A1 (en) | 2007-02-27 | 2008-10-16 | Deka Products Limited Partnership | Pumping Cassette |
| KR101334183B1 (ko) | 2007-06-01 | 2013-12-02 | 삼성전자주식회사 | 미세 반응을 위한 형광 검출 모듈과 이를 구비한 형광 검출시스템 |
| JP2009014529A (ja) | 2007-07-05 | 2009-01-22 | Panasonic Corp | 分析用デバイス、分析用デバイスを用いる分析装置、および液体試料成分測定方法 |
| JP2009019962A (ja) | 2007-07-11 | 2009-01-29 | Konica Minolta Medical & Graphic Inc | 蛍光検出ユニット、反応検出装置、マイクロチップ検査システム |
| WO2009018473A1 (fr) | 2007-07-31 | 2009-02-05 | Micronics, Inc. | Système de récupération d'écouvillon sanitaire, dispositif d'analyse microfluidique et procédés pour des analyses de diagnostic |
| EP2040073A1 (fr) | 2007-09-20 | 2009-03-25 | Iline Microsystems, S.L. | Dispositif microfluidique et procédé pour la détermination du temps de coagulation d'un fluide |
| US7906317B2 (en) | 2007-10-21 | 2011-03-15 | King Car Food Industrial Co., Ltd. | Apparatus for thin-layer cell smear preparation and in-situ hybridization |
| AU2009217355A1 (en) | 2008-02-21 | 2009-08-27 | Avantra Biosciences Corporation | Assays based on liquid flow over arrays |
| EP2318549B1 (fr) | 2008-08-15 | 2013-06-26 | Cascade Biosystems, Inc. | Détection d'acide nucléique |
| WO2010027812A2 (fr) | 2008-08-25 | 2010-03-11 | University Of Washington | Systèmes microfluidiques comprenant des membranes à écoulement continu |
| WO2010025302A2 (fr) | 2008-08-27 | 2010-03-04 | Life Technologies Corporation | Appareil et procédé utilisables pour le traitement d'échantillons biologiques |
| JP2010078508A (ja) | 2008-09-26 | 2010-04-08 | Toshiba Corp | 送液装置 |
| US8318439B2 (en) | 2008-10-03 | 2012-11-27 | Micronics, Inc. | Microfluidic apparatus and methods for performing blood typing and crossmatching |
| EP2202328A1 (fr) | 2008-12-26 | 2010-06-30 | Fundacion Inasmet | Processus pour obtenir un revêtement protecteur pour hautes températures avec rugosité élevée et revêtement obtenu |
| JP5715068B2 (ja) | 2009-01-30 | 2015-05-07 | マイクロニクス， インコーポレイテッド | 携帯型高利得蛍光検出システム |
| WO2010111265A1 (fr) | 2009-03-24 | 2010-09-30 | University Of Chicago | Dispositif et procédés de puce coulissante |
| CN102448612B (zh) | 2009-04-13 | 2014-12-03 | 精密公司 | 微流体临床分析器 |
| CN102459565A (zh) | 2009-06-02 | 2012-05-16 | 尹特根埃克斯有限公司 | 具有隔膜阀的流控设备 |
| CN102803507B (zh) | 2009-06-12 | 2016-05-25 | 精密公司 | 用于微流体装置中在板反应剂的脱水储存的组合物及方法 |
| JP5116114B2 (ja) | 2009-07-27 | 2013-01-09 | 富士フイルム株式会社 | マイクロ化学装置の運転方法 |
| WO2012071069A1 (fr) | 2010-11-23 | 2012-05-31 | Cyvek, Inc. | Procédé et appareil permettant de réaliser des dosages |
| US9132423B2 (en) | 2010-01-29 | 2015-09-15 | Micronics, Inc. | Sample-to-answer microfluidic cartridge |
| AT509622A1 (de) | 2010-04-08 | 2011-10-15 | Greiner Bio One Gmbh | Verfahren und kit zur trennung viraler und prokaryontischer von eukaryontischen nukleinsäuren |
| TW201217783A (en) | 2010-09-15 | 2012-05-01 | Mbio Diagnostics Inc | System and method for detecting multiple molecules in one assay |
| EP2471652A1 (fr) | 2010-12-28 | 2012-07-04 | Nordenia Technologies GmbH | Matière en couche élastomère et son procédé de fabrication |
| EP2676136B1 (fr) * | 2011-02-15 | 2020-12-23 | Hemosonics, Llc | Dispositifs, systèmes et procédés pour l'évaluation d'une hémostase |
| US20140158619A1 (en) | 2011-07-20 | 2014-06-12 | Qiagen Gmbh | Filtering devices comprising clay minerals |
| US20130032235A1 (en) | 2011-08-02 | 2013-02-07 | Teledyne Dalsa Semiconductor, Inc. | Integrated microfluidic check valve and device including such a check valve |
| CA2849104A1 (fr) * | 2011-09-25 | 2013-04-11 | Theranos, Inc. | Systemes et procedes pour multi-analyse |
| JP6190822B2 (ja) | 2012-01-09 | 2017-08-30 | マイクロニクス， インコーポレイテッド | マイクロ流体リアクタシステム |
| US10518262B2 (en) | 2012-12-21 | 2019-12-31 | Perkinelmer Health Sciences, Inc. | Low elasticity films for microfluidic use |
| JP2016509206A (ja) | 2012-12-21 | 2016-03-24 | マイクロニクス， インコーポレイテッド | 携帯型蛍光検出システムおよびマイクロアッセイカートリッジ |
| KR102102123B1 (ko) | 2012-12-21 | 2020-04-20 | 퍼킨엘머 헬스 사이언시즈, 아이엔씨. | 유체 공학 회로 및 관련 제조 방법 |
| JP6484222B2 (ja) | 2013-05-07 | 2019-03-13 | マイクロニクス， インコーポレイテッド | 核酸の調製および分析のためのデバイス |
| US10190153B2 (en) | 2013-05-07 | 2019-01-29 | Micronics, Inc. | Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions |
| JP2017520239A (ja) | 2014-06-11 | 2017-07-27 | マイクロニクス， インコーポレイテッド | 核酸の分析のための統合されたアッセイ対照を備えたマイクロ流体カートリッジおよび装置 |
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| JP2016523520A (ja) | 2016-08-12 |
| CN105189784B (zh) | 2020-06-30 |
| EP2994543A4 (fr) | 2017-01-04 |
| CN105189784A (zh) | 2015-12-23 |
| AU2014262726B2 (en) | 2019-09-19 |
| US20160090588A1 (en) | 2016-03-31 |
| US10087440B2 (en) | 2018-10-02 |
| WO2014182847A1 (fr) | 2014-11-13 |
| AU2014262726A1 (en) | 2015-11-12 |
| EP2994543A1 (fr) | 2016-03-16 |
| EP2994543B1 (fr) | 2018-08-15 |
| JP6484222B2 (ja) | 2019-03-13 |
| CA2911308C (fr) | 2021-10-19 |
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