CA2794800A1 - Procede d'amelioration de la detection de la recombinaison de genes d'immunoglobulines de lymphocytes b - Google Patents

Procede d'amelioration de la detection de la recombinaison de genes d'immunoglobulines de lymphocytes b Download PDF

Info

Publication number
CA2794800A1
CA2794800A1 CA2794800A CA2794800A CA2794800A1 CA 2794800 A1 CA2794800 A1 CA 2794800A1 CA 2794800 A CA2794800 A CA 2794800A CA 2794800 A CA2794800 A CA 2794800A CA 2794800 A1 CA2794800 A1 CA 2794800A1
Authority
CA
Canada
Prior art keywords
amplification
primers
primer
pcr
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2794800A
Other languages
English (en)
Inventor
Jian Han
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2794800A1 publication Critical patent/CA2794800A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2794800A 2010-03-29 2011-03-29 Procede d'amelioration de la detection de la recombinaison de genes d'immunoglobulines de lymphocytes b Abandoned CA2794800A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31841710P 2010-03-29 2010-03-29
US61/318,417 2010-03-29
PCT/US2011/030398 WO2011123473A1 (fr) 2010-03-29 2011-03-29 Procédé d'amélioration de la détection de la recombinaison de gènes d'immunoglobulines de lymphocytes b

Publications (1)

Publication Number Publication Date
CA2794800A1 true CA2794800A1 (fr) 2011-10-06

Family

ID=44712594

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2794800A Abandoned CA2794800A1 (fr) 2010-03-29 2011-03-29 Procede d'amelioration de la detection de la recombinaison de genes d'immunoglobulines de lymphocytes b

Country Status (6)

Country Link
EP (1) EP2558479A4 (fr)
JP (1) JP2013523129A (fr)
CN (1) CN103154015A (fr)
AU (1) AU2011235281A1 (fr)
CA (1) CA2794800A1 (fr)
WO (1) WO2011123473A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104388579B (zh) * 2014-12-16 2018-11-09 上海速芯生物科技有限公司 一种转基因大豆及其衍生品种的Arm-PCR检测方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5106727A (en) * 1989-04-27 1992-04-21 Life Technologies, Inc. Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers
AU2001232485A1 (en) * 2000-01-13 2001-07-24 Amsterdam Support Diagnostics B.V. A universal nucleic acid amplification system for nucleic acids in a sample

Also Published As

Publication number Publication date
AU2011235281A1 (en) 2012-11-22
WO2011123473A1 (fr) 2011-10-06
JP2013523129A (ja) 2013-06-17
EP2558479A4 (fr) 2013-09-18
EP2558479A1 (fr) 2013-02-20
CN103154015A (zh) 2013-06-12

Similar Documents

Publication Publication Date Title
US20210310076A1 (en) Methods of monitoring conditions by sequence analysis
Mamedov et al. Preparing unbiased T-cell receptor and antibody cDNA libraries for the deep next generation sequencing profiling
RU2578009C2 (ru) Способ идентификации нативных пар фрагментов днк или рнк, присутствующих в одних и тех же живых клетках
Turchaninova et al. Pairing of T‐cell receptor chains via emulsion PCR
Dziubianau et al. TCR repertoire analysis by next generation sequencing allows complex differential diagnosis of T cell–related pathology
Bonarius et al. Monitoring the T-cell receptor repertoire at single-clone resolution
US20150031555A1 (en) Method for correction of bias in multiplexed amplification
Lim et al. V-gene amplification revisited–An optimised procedure for amplification of rearranged human antibody genes of different isotypes
CA2794800A1 (fr) Procede d'amelioration de la detection de la recombinaison de genes d'immunoglobulines de lymphocytes b
Mugnaini et al. Molecular analysis of the complementarity determining region 3 of the human T cell receptor β chain. Establishment of a reference panel of CDR3 lengths from phytohaemagglutinin activated lymphocytes
RU2600873C1 (ru) Способ идентификации нативных пар фрагментов днк или рнк, присутствующих в одних и тех же живых клетках
US20240287606A1 (en) Immume cell counting based on immune repertoire sequencing
Ford et al. FLAIRR-seq: A novel method for single molecule resolution of near full-length immunoglobulin heavy chain repertoires
Tu Recovery of T cell receptor variable sequences from 3'barcoded single-cell RNA sequencing libraries
Lassen et al. A novel DNAseq program for enhanced analysis of Illumina GAII data: a case study on antibody complementarity-determining regions

Legal Events

Date Code Title Description
FZDE Dead

Effective date: 20150331