CA2700945A1 - Nucleotide triphosphate with an electroactive label conjugated to the gamma phosphate - Google Patents

Nucleotide triphosphate with an electroactive label conjugated to the gamma phosphate Download PDF

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Publication number
CA2700945A1
CA2700945A1 CA2700945A CA2700945A CA2700945A1 CA 2700945 A1 CA2700945 A1 CA 2700945A1 CA 2700945 A CA2700945 A CA 2700945A CA 2700945 A CA2700945 A CA 2700945A CA 2700945 A1 CA2700945 A1 CA 2700945A1
Authority
CA
Canada
Prior art keywords
kinase
substrate
phosphorylation
electroactive
gamma phosphate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2700945A
Other languages
English (en)
French (fr)
Inventor
Kagan Kerman
Haifeng Song
Heinz-Bernhard Kraatz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Western Ontario
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2700945A1 publication Critical patent/CA2700945A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/30—Electrochemically active labels

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
CA2700945A 2007-09-27 2008-09-26 Nucleotide triphosphate with an electroactive label conjugated to the gamma phosphate Abandoned CA2700945A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96038807P 2007-09-27 2007-09-27
US60/960,398 2007-09-27
PCT/CA2008/001690 WO2009039639A1 (en) 2007-09-27 2008-09-26 Nucleotide triphosphate with an electroactive label conjugated to the gamma phosphate

Publications (1)

Publication Number Publication Date
CA2700945A1 true CA2700945A1 (en) 2009-04-02

Family

ID=40510705

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2700945A Abandoned CA2700945A1 (en) 2007-09-27 2008-09-26 Nucleotide triphosphate with an electroactive label conjugated to the gamma phosphate

Country Status (4)

Country Link
EP (1) EP2195447A4 (zh)
CN (1) CN102016064A (zh)
CA (1) CA2700945A1 (zh)
WO (1) WO2009039639A1 (zh)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9063156B2 (en) 2009-06-12 2015-06-23 Pacific Biosciences Of California, Inc. Real-time analytical methods and systems
CN105548301B (zh) * 2016-01-28 2018-07-31 中南大学 一种测定蛋白激酶活性的方法和蛋白激酶抑制剂活性的方法
CN109030583B (zh) * 2018-08-28 2023-09-15 长沙理工大学 基于2-巯基苯并咪唑的l-半胱氨酸的检测方法及传感器
CN111693707A (zh) * 2020-07-16 2020-09-22 首都医科大学附属北京朝阳医院 Rock激酶活性在辅助诊断血管炎患者中的应用
CN114235922A (zh) * 2020-09-09 2022-03-25 中国科学院大连化学物理研究所 一种利用功能纳米通道实时监测磷酸化的方法
CN113430248B (zh) * 2021-06-28 2022-11-11 中国人民解放军军事科学院军事医学研究院 一种新型p53蛋白激酶的非同位素筛选方法及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6360500A (en) * 1999-07-20 2001-02-05 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
US7365178B2 (en) * 2003-04-01 2008-04-29 Activx Biosciences, Inc. Acyl-nucleotide probes and methods of their synthesis and use in proteomic analysis

Also Published As

Publication number Publication date
EP2195447A4 (en) 2011-03-30
WO2009039639A8 (en) 2009-05-07
WO2009039639A1 (en) 2009-04-02
EP2195447A1 (en) 2010-06-16
CN102016064A (zh) 2011-04-13

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20130926

FZDE Discontinued

Effective date: 20150928