CA2523442A1 - Molecular detection systems utilizing reiterative oligonucleotide synthesis - Google Patents

Molecular detection systems utilizing reiterative oligonucleotide synthesis Download PDF

Info

Publication number
CA2523442A1
CA2523442A1 CA002523442A CA2523442A CA2523442A1 CA 2523442 A1 CA2523442 A1 CA 2523442A1 CA 002523442 A CA002523442 A CA 002523442A CA 2523442 A CA2523442 A CA 2523442A CA 2523442 A1 CA2523442 A1 CA 2523442A1
Authority
CA
Canada
Prior art keywords
alkyl
target
aminocarbonyl
oligonucleotide
initiator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002523442A
Other languages
English (en)
French (fr)
Inventor
Michelle M. Hanna
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ribomed Biotechnologies Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2523442A1 publication Critical patent/CA2523442A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002523442A 2003-04-29 2004-04-29 Molecular detection systems utilizing reiterative oligonucleotide synthesis Abandoned CA2523442A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10/425,037 2003-04-29
US10/425,037 US20040054162A1 (en) 2001-10-30 2003-04-29 Molecular detection systems utilizing reiterative oligonucleotide synthesis
PCT/US2004/013031 WO2004096997A2 (en) 2003-04-29 2004-04-29 Molecular detection systems utilizing reiterative oligonucleotide synthesis

Publications (1)

Publication Number Publication Date
CA2523442A1 true CA2523442A1 (en) 2004-11-11

Family

ID=33415910

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002523442A Abandoned CA2523442A1 (en) 2003-04-29 2004-04-29 Molecular detection systems utilizing reiterative oligonucleotide synthesis

Country Status (6)

Country Link
US (2) US20040054162A1 (https=)
EP (1) EP1622923A4 (https=)
JP (1) JP2006525022A (https=)
AU (1) AU2004235368A1 (https=)
CA (1) CA2523442A1 (https=)
WO (1) WO2004096997A2 (https=)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
EP1368497A4 (en) 2001-03-12 2007-08-15 California Inst Of Techn METHODS AND APPARATUS FOR ANALYZING ASYNCHRONOUS BASE EXTENSION POLYNUCLEOTIDE SEQUENCES
US9261460B2 (en) * 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
DE10154317B4 (de) * 2001-10-26 2005-06-09 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
US20040054162A1 (en) * 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
US7045319B2 (en) * 2001-10-30 2006-05-16 Ribomed Biotechnologies, Inc. Molecular detection systems utilizing reiterative oligonucleotide synthesis
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
WO2005042709A2 (en) * 2003-10-29 2005-05-12 Ribomed Biotechnologies, Inc. Compositions, methods and detection technologies for reiterative oligonucleotide synthesis
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20060172408A1 (en) * 2003-12-01 2006-08-03 Quake Steven R Device for immobilizing chemical and biochemical species and methods of using same
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
DE602005027700D1 (de) 2004-05-25 2011-06-09 Helicos Biosciences Corp Verfahren zur nukleinsäureimmobilisierung
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
WO2006037102A2 (en) * 2004-09-27 2006-04-06 Epicentre Technologies Methods for identifying polymerase inhibitors
WO2006036943A2 (en) 2004-09-27 2006-04-06 The University Of North Carolina At Chapel Hill Determination of molecular age by detection of ink4a/arf expression
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
DE602006018622D1 (de) * 2005-06-30 2011-01-13 Ge Healthcare Bio Sciences Nachweisverfahren für die genexpression
US20070031875A1 (en) * 2005-08-05 2007-02-08 Helicos Biosciences Corporation Signal pattern compositions and methods
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
CA2724343A1 (en) 2008-05-15 2009-11-19 Ribomed Biotechnologies, Inc. Methods and reagents for detecting cpg methylation with a methyl cpg binding protein (mbp)
WO2010009060A2 (en) * 2008-07-13 2010-01-21 Ribomed Biotechnologies, Inc. Molecular beacon-based methods for detection of targets using abscription
RU2011141720A (ru) 2009-03-15 2013-04-27 Рибомед Байотекнолоджиз, Инк. Молекулярное обнаружение, основанное на абскрипции
WO2011034895A1 (en) * 2009-09-15 2011-03-24 Regents Of The University Of Colorado Compositions, methods and uses for nucleotide analogues
US8809531B2 (en) * 2010-04-02 2014-08-19 Pharmacophotonics, Inc. Rhodamine dyes and conjugates
ES3018861T3 (en) * 2011-12-13 2025-05-19 Univ Oslo Hf Method for detection of hydroxymethylation status
WO2015164602A2 (en) * 2014-04-23 2015-10-29 Children's Medical Center Corporation High-throughput structure determination using nucleic acid calipers
CA3069985A1 (en) * 2017-07-24 2019-01-31 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
CN108467889A (zh) * 2018-04-04 2018-08-31 苏州创澜生物科技有限公司 一种用于多重pcr的荧光探针及其应用
AU2019300169A1 (en) 2018-07-13 2021-01-28 Quantum-Si Incorporated Biconjugatable labels and methods of use
MX2021008867A (es) 2019-01-23 2021-08-19 Quantum Si Inc Composiciones reactantes marcadas de intensidad alta y metodos de secuenciamiento.
US11591629B2 (en) * 2019-12-23 2023-02-28 Cheng-Yao Chen Method and kit for template-independent nucleic acid synthesis
WO2022023753A1 (en) 2020-07-30 2022-02-03 Cambridge Epigenetix Limited Compositions and methods for nucleic acid analysis

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3996345A (en) * 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4351760A (en) * 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US4582788A (en) * 1982-01-22 1986-04-15 Cetus Corporation HLA typing method and cDNA probes used therein
US4786600A (en) * 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
US5503979A (en) * 1984-05-25 1996-04-02 The Trustees Of Columbia University In The City Of New York Method of using replicatable hybridzable recombinant RNA probes
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4739044A (en) * 1985-06-13 1988-04-19 Amgen Method for derivitization of polynucleotides
US4757141A (en) * 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5508178A (en) * 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
US5766849A (en) * 1989-07-11 1998-06-16 Gen-Probe Incorporated Methods of amplifying nucleic acids using promoter-containing primer sequence
CA2020958C (en) * 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US6114513A (en) * 1990-01-11 2000-09-05 Isis Pharmaceuticals, Inc. Thiol-derivatized oligonucleotides
US5578718A (en) * 1990-01-11 1996-11-26 Isis Pharmaceuticals, Inc. Thiol-derivatized nucleosides
US5194370A (en) * 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
US5595891A (en) * 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
US5888819A (en) * 1991-03-05 1999-03-30 Molecular Tool, Inc. Method for determining nucleotide identity through primer extension
US5169766A (en) * 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
JP4137996B2 (ja) * 1992-05-06 2008-08-20 ジェン−プローブ・インコーポレイテッド 核酸配列増幅方法，組成物及びキット
WO1994001445A1 (en) * 1992-07-14 1994-01-20 Research Corporation Technologies, Inc. Base-protected nucleotide analogs with protected thiol groups
DE69310179T2 (de) * 1992-07-31 1997-07-31 Behringwerke Ag Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden
ZA936016B (en) * 1992-08-24 1994-03-10 Akzo Nv Method for nucleic acid amplification
US5571669A (en) * 1993-01-14 1996-11-05 The University Of Pennsylvania Transcription and nucleic acid sequence determination with short primer DNA/RNA molecules and RNA polymerase
US5597694A (en) * 1993-10-07 1997-01-28 Massachusetts Institute Of Technology Interspersed repetitive element-bubble amplification of nucleic acids
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
FR2724934B1 (fr) * 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
US5684142A (en) * 1995-06-07 1997-11-04 Oncor, Inc. Modified nucleotides for nucleic acid labeling
FR2737223B1 (fr) * 1995-07-24 1997-09-12 Bio Merieux Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
US5912340A (en) * 1995-10-04 1999-06-15 Epoch Pharmaceuticals, Inc. Selective binding complementary oligonucleotides
AU3891097A (en) * 1996-07-24 1998-02-10 Michelle M. Hanna Base-protected nucleotide analogs with protected thiol groups
US5849546A (en) * 1996-09-13 1998-12-15 Epicentre Technologies Corporation Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates
US5846723A (en) * 1996-12-20 1998-12-08 Geron Corporation Methods for detecting the RNA component of telomerase
US5858801A (en) * 1997-03-13 1999-01-12 The United States Of America As Represented By The Secretary Of The Navy Patterning antibodies on a surface
US6251594B1 (en) * 1997-06-09 2001-06-26 Usc/Norris Comprehensive Cancer Ctr. Cancer diagnostic method based upon DNA methylation differences
US6114519A (en) * 1997-10-15 2000-09-05 Isis Pharmaceuticals, Inc. Synthesis of sulfurized oligonucleotides
US6268131B1 (en) * 1997-12-15 2001-07-31 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids
US6803485B2 (en) * 1999-02-26 2004-10-12 Bracco Imaging S.P.A. Process for the preparation of iopamidol
WO2001020017A2 (en) * 1999-09-14 2001-03-22 Yeda Research And Development Co. Ltd. Metal cluster containing nucleotides and nucleic acids, and intermediates therefor
US20020168641A1 (en) * 2001-03-09 2002-11-14 Bruce Mortensen Fluorescein-cyanine 5 as a fluorescence resonance energy transfer pair
US20040054162A1 (en) * 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
US7045319B2 (en) * 2001-10-30 2006-05-16 Ribomed Biotechnologies, Inc. Molecular detection systems utilizing reiterative oligonucleotide synthesis
WO2005042709A2 (en) * 2003-10-29 2005-05-12 Ribomed Biotechnologies, Inc. Compositions, methods and detection technologies for reiterative oligonucleotide synthesis

Also Published As

Publication number Publication date
EP1622923A2 (en) 2006-02-08
WO2004096997A3 (en) 2005-09-15
US20060204964A1 (en) 2006-09-14
US20040054162A1 (en) 2004-03-18
JP2006525022A (ja) 2006-11-09
EP1622923A4 (en) 2007-11-28
WO2004096997A2 (en) 2004-11-11
AU2004235368A1 (en) 2004-11-11

Similar Documents

Publication Publication Date Title
CA2465158C (en) Molecular detection systems utilizing reiterative oligonucleotide synthesis
US20060204964A1 (en) Molecular detection systems utilizing reiterative oligonucleotide synthesis
JP3929775B2 (ja) ポリヌクレオチド配列の線形等温増幅のための方法および組成物
US8551709B2 (en) Methods for fragmentation and labeling of nucleic acids
US9175348B2 (en) Identification of 5-methyl-C in nucleic acid templates
JP2004512843A (ja) 核酸の増幅および任意のキャラクタリゼーションの方法
AU5882000A (en) Improved primers and methods for the detection and discrimination of nucleic acids
NZ527128A (en) Primers and methods for the detection and discrimination of nucleic acids
KR20230080414A (ko) 샘플에서 하나 이상의 표적 분석물을 검출하기 위한 키트 및 이의 제조 및 사용 방법
JP2007125014A (ja) 遺伝子メチル化検査法対照物
AU2002248257A1 (en) Primers and methods for the detection and discrimination of nucleic acids

Legal Events

Date Code Title Description
FZDE Dead