CA2474940A1 - Method and device to process, label and concentrate analytes - Google Patents

Method and device to process, label and concentrate analytes

Info

Publication number
CA2474940A1
CA2474940A1 CA 2474940 CA2474940A CA2474940A1 CA 2474940 A1 CA2474940 A1 CA 2474940A1 CA 2474940 CA2474940 CA 2474940 CA 2474940 A CA2474940 A CA 2474940A CA 2474940 A1 CA2474940 A1 CA 2474940A1
Authority
CA
Grant status
Application
Patent type
Prior art keywords
outlet
piston
syringe
hollow bore
applicator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2474940
Other languages
French (fr)
Inventor
Peter Lea
Nicole Szabados Haynes
Shi-Fa Ding
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biophys Inc
Original Assignee
Biophys Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42Low-temperature sample treatment, e.g. cryofixation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/4044Concentrating samples by chemical techniques; Digestion; Chemical decomposition
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/405Concentrating samples by adsorption or absorption
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38Diluting, dispersing or mixing samples
    • G01N2001/386Other diluting or mixing processes
    • G01N2001/388Other diluting or mixing processes mixing the sample with a tracer

Abstract

A sample applicator for transferring a fluid sample, includes a syringe body defining a hollow bore with an open end and an outlet, a plunger having a piston at one end and being effective to fit within the hollow bore, and a second end extending out of the open end when the piston is located within the hollow bore. The syringe applicator further includes at least one of a reagent and a concentrating material and a fluid dye material, located between the piston and the outlet. The contents of the sample applicator are lyophilized within the sample applicator.

Description

METHOD AND DEVICE TO PROCESS, LABEL AND CONCENTRATE ANALYTES
Field of the Invention The present invention relates to a device and methods for the preparation, in fluid samples, for the detection and quantification of an analyte that may be present in the fluid sample.
Background of the Invention Micro-organisms, including toxic bacteria, yeast and mould account far several million cases of food-related illnesses and 9,000 deaths per year in the United States alone. Contaminated poultry and meat products are a major cause of these deaths and illness. The four most common pathogens infecting poultry and meat products are E. coli 0157:H7, Campylobacter jejunilcoli, Salmonella species and Listeria species and L.
monocytogenes.
Contaminated water supplies are also a health hazard. The United States 1 S Environmental Protection Agency has determined that the level of E. coli in water supply is a good indicator of health risk. Other common indicators are total coliforms, fecal coliforms, fecal streptococci and enterococci.
Many disease conditions, such as bacterial and viral infections, cancers, heart attacks and strokes for example, may be detected through the testing of blood and tissues, including interstitial fluid, saliva, urine, semen, feces, genetic coding and tissue sections, for markers that are associated with particular conditions. Early and rapid diagnosis and measurement of treatment efficacy is desired to enhance successful treatment.
The accurate quantitation and rapid detection of micro-organisms, such as bacteria, viruses, fungi or other infectious organisms and indicators in food and water, on surfaces where food is prepared and on other surfaces that should meet sanitary standards is a pressing need. There is also a serious need for the accurate and rapid identification of micro-organisms and disease markers of a patient.
In a typical test assay, a fluid sample is mixed with a reagent, such as an antibody, specific to a particular analyte, such as an antigen. The reaction of the analyte with the reagent may result in a color change that may be visually observed, or in chemiluminescent, bioluminescent or fluorescent species that may be observed with a microscope or detected by a photodetecting device, such as a spectrophotometer. The reagent may also be a fluorescent or other such detectable labelling reagent that binds to S the analyte. Irradiation scattered, reflected, transmitted or absorbed by the fluid sample may also be indicative of the identity and type of analyte in the fluid sample.
In a commonly used assay technique, two types of antibodies are used, both specific to the analyte. One type of antibody is immobilized on a solid support. The other type of antibody is labelled by conjugation with a detectable marker and mixed with the sample. A complex between the first antibody, the substance being tested for and the second antibody is formed, immobilizing the marker. The label to identify the specific marker may also be an enzyme or a fluorescent or radioactive marker, which may then be detected. See, for example, U.S. Patent No. 5,610,077.
Fluid samples must often be grown in culture medium to increase the 1 S concentration of the analyte to a level that may be reliably detected. The fluid sample may then be transferred to a container, such as a test tube, for mixing with the reagents.
The fluid sample rnay then be transferred to an assay device for analysis or to another container for mixing with other reagents. Such transfers, which are often conducted with a pipette axe time consuming, labour intensive and expose the fluid sample and the people conducting the analysis to contamination. There is a need for a more convenient aseptic method to transfer fluid samples and to mix fluid samples with reagents and other compounds required for testing.
Summary of the Invention The invention provides a sample labelling applicator device for transferring a fluid 2S sample, including a syringe body defining a hollow bore with an open end and an outlet, a plunger having a piston at one end and being effective to fit within the hollow bore, and a second end extending out of the open end where the piston is located within the hollow bore; and a reagent located between the piston and the outlet where the piston is located within the hollow bore.

According to another aspect of the present invention, there is provided a method for concentrating an analyte present in a liquid sample, including a syringe body defining a hollow bore with an open end and an outlet, a plunger having a piston at one end and being effective to fit within the hollow bore, and a second end extending out of the open end where the piston is located within the hollow bore; and a reagent located between the piston and the outlet where the piston is located within the hollow bore;
mixing and incubating the analyte and the reagent for a period of time sufficient to react the analyte with the reagent and expelling a controlled amount of the portion through the outlet.
According to another aspect of the present invention, there is provided a method for reacting an analyte present in a liquid sample, including a syringe body defining a hollow bore with an open end and an outlet, a plunger having a piston at one end and being effective to fit within the hollow bore, and a second end extending out of the open end where the piston is located within the hollow bore; and a reagent located between the piston and the outlet where the piston is located within the hollow bore, mixing and incubating the portion with the reagent for a period of time sufficient to react the analyte in the portion with the reagent; and expelling an amount of the portion through the outlet.
Brief Description of the Drawings Figure 1 is a front perspective view of a sample applicator of the present invention;
Figure 2 is a cross-sectional view of the sample applicator taken along lines 2-2 of Figure 1; and Figure 3 is a perspective view of a cap of the present invention detached from the sample applicator.
Detailed Description of the Invention Sample applicator 10 is shown in Figure 1. The sample applicator 10 comprises a syringe body 12, which is preferably cylindrical in shape. The syringe body 12 has a first end 62 and a second end 64. The syringe body 12 defines a hollow bore 14 with a first open end 16. A plunger I8 has a piston 20 at its first end within the bore 14 and a second end 21 extending out of the first open end 16 of the bore 14. The second end 21 is preferably widened for engagement by a user. The syringe body 12 defines an outlet 22 at the second end 64 of the syringe body 12. A flange 24 is attached to the second end 64 of the syringe body 12. The flange 24 is preferably cylindrical. The flange defines outlet 26 that is in fluid communication with the outlet 22 of the syringe body 12.
The outer surface of the flange 24 may have the shape of a Luer lock or taper. Reagents 43, and preferably a concentrating material 45 to facilitate the analysis of the fluid sample, are provided between the plunger 20 and the outlet 22. The concentrating material 45 is preferably super absorbent polymer particles. The reagents are preferably monoclonal antibodies that are specific for a given analyte. Most preferably the reagents are lyophilized monoclonal antibodies that rapidly reconstitute upon mixing with a sample fluid. The reagents may also be frozen, in liquid form or may be dried on the interior surfaces of the syringe body 12.
Volume indicator graduation lines 47, 49 are preferably provided for ease of use.
On a 1 ml syringe body, the first volume line 47 may correspond to drawing 200 ml from the source and the second volume line 49 may correspond to drawing an additional 100-150 ml into the syringe bore 14, for example.
The syringe applicator 10 includes a preferably cylindrical inlet/outlet assembly comprising an upper region 80 and a lower region 82. The inner diameter of the upper portion 80 is about the same as or is slightly larger than the outer diameter of the lower 20 portion 82. The upper region 80 has a first end 46 coupled to the second end 64 of the syringe body 12. The lower region has an end 48 being for receiving a fluid sample. The inlet/outlet assembly 25 defines an opening 42 at the end 48 for passage of sample fluid.
The inlet/outlet assembly 25 is provided to extend the distance from the flange 24 to the source for ease of use and to avoid insertion of syringe body 12 into the source. Fluid 25 sample on the syringe body could contaminate the user and the surroundings.
A length L for the inlet/outlet assembly 25 of at least about 2 cm is preferred whereas 3 cm is more preferred. A preferred range is from about 2 cm to about 7 cm.
The configuration of the inlet/outlet assembly 25 may vary according to the application to be carried out using the syringe applicator 10.

A cap 30 is preferably attached to the end 48 of lower portion 82 of the inletloutlet assembly 2S thereby covering the opening 42 and sealing the syringe applicator 10 to prevent contamination prior to use. The cap 30 preferably has a first outwardly tapering cone-shaped cylindrical shaped section and a flat tip 76. The cap 30 is preferably attached S to the inlet/outlet assembly 2S by means of perforations 70. The cap must therefore be broken off from the inlet/outlet assembly 2S. An intact attached cap indicates that the syringe applicator 10 ha not been used. The cap 30 is adapted to engage the lower region 82 in a fluid tight fit. The cap 30 is preferably a rigid moulded plastic such as polystyrene, an acrylic or polypropylene. A semi-rigid material may also be used in alternate embodiments.
To use the sample applicator 10 of the present invention, the cap 30 is torn off the lower region 82 of the inlet/outlet assembly 2S and a fluid sample is drawn from a fluid source. The fluid source may be a culture medium or media, depending on the concentration of the analyte in the fluid sample and the nature of the source matrix. For 1 S example, if the source is a food sample, culturing is typically required.
If the source is feces, culturing may not be necessary. The lower region 82 is inserted into the source and the plunger 18 is withdrawn to draw a fluid sample from the source through the inlet/outlet assembly 2S and into the bore 14 of the syringe body 12. About 300-350 p1 is an appropriate amount, for example. If the volume lines 47 and 49 are provided, the first volume 47 is positioned so that an appropriate amount of the fluid sample is drawn into the syringe applicator when plunger 18 is withdrawn up to the first volume line 47. The sample applicator 10 is then removed from the source and the plunger 18 is preferably further withdrawn, to the second volume line 49. The cap 30 is placed over the inlet/outlet assembly 2S to cap and seal the fluid inside the syringe body 12.
The syringe 2S applicator 10 is then inverted several times to reconstitute the reagents 43, and to adequately mix the reagents with the fluid sample. If the concentrating material 4S is present, the fluid sample is allowed to incubate for about 20 minutes. If the concentrating material 4S is not present, about S minutes of incubation is sufficient to reconstitute the reagents which are typically lyophilized antibodies. After sufficient incubation, the cap 30 is removed and the plunger is advanced to expel a sufficient amount of the fluid sample from the bore 14 through the inlet/outlet assembly 2S and into an assay device for analysis. Instead of being transferred to an assay device, the fluid sample may be -S-transferred to another location, such as into another container for reaction with other reagents.
If only the concentrating material 45 is provided in the syringe body 12, the fluid sample may be transferred into a reaction chamber for reaction with reagents.
Even if reagents are present in the reaction chamber, further reaction with additional reagents may be required.
The fluid sample once exposed to the reagents in the sample applicator 10 may be transferred to an assay device to be analyzed for the presence of analytes.
The analyte may be a microbe such as a bacterium, a virus or a fungus, for example. The analyte may also be a biological substance such as a peptide, an amino acid, a nucleic acid, or a protein, such as an enzyme, an antibody, an antigen, an immuno-globulin or a hormone, for example. The analyte may also be a chemical substance such as a chemical element, chemical compound or a polymer, for example. The fluid sample may also be transferred to a reaction vessel. The source of the fluid sample may be a culture medium containing a suspect food, water or fecal matter, for example. The source may be uncultured, as well.
The sample applicator 10 includes reagents for reacting with and enabling the identification of an analyte in the fluid sample. The reagents may be labelled with a fluorescent chemical, colorimetric or radioactive label, for example, as is known in the art. The label can also be a latex bead, metal colloidal particle, dye, enzyme or Stoke effect transducer. Other types of reagents may also be used, as is known in the art.
If the analyte is present and the reagents are antibodies, the reagent antibodies bind to the analyte and label the analyte with the detectable marker. The fluid sample and the labelled analyte may then be transferred to an appropriate assay device for analysis. A
preferred assay device disclosed in U.S. Patent Application Publication No.

entitled "Assay Devices", which is incorporated by reference herein in its entirety.
In one application, the analyte is E. toll 0157:H7. The sample applicator contains lyophilized monoclonal mouse-anti-E. toll 0157:H7 antibody, available from Biodesign International, Saco, Maine, U.S.A., for example. The antibodies may be pre-conjugated with Alexa Fluor~ 647 fluorescent dye available from Molecular Probes Incorporated, Eugene, Ohio, U.S.A., for example. Molecular Probes also provides an Alexa Fluor~

647 Protein Labeling Kit (A-20173) for conjugating the antibodies to the dye.
The labelled antibodies are preferably lyophilized as is known in the art.
Alternatively, frozen labelled antibodies may be used. In another alternative, an aliquot of the labelled antibodies in a liquid suspension may be drawn into or otherwise placed within the syringe body and the sample applicator 10 may be placed in a freezer to freeze the suspension. The sample applicator 10 may be used after removal from the freezer.
In another example, the reagents may be a luciferin/luciferase complex for detecting ATP as an indicator of the presence of biological contamination of a sample.
ATP reacts with a luciferin/luciferase complex, in the presence of oxygen and magnesium ions (Mg*2 ) to produce visible light at a wavelength of 562 nanometers. Since all living cells contain ATP, detection of light in S62 nanometers is indicative of the presence of living cells. Luciferin/luciferase is commonly extracted from fireflies.
Luciferin/luciferase reagents are commercially available. An Adenosine 5' Triphosphate (ATP) Bioluminescent Assay Kit, FL-AAM, available from the Sigma-Aldrich Corporation, Milwaukee, Wisconsin, U.S.A., may be used, for example. FL-AAM is a lyophilized powder containing firefly luciferase, luciferin, MgS04 , EDTA, dithiothreitol ("DTT") and bovine serum albumin ("BSA") in a tricine buffer. After mixing with the reagents, the fluid sample can be transferred to an appropriate assay device for detection of luminescent species resulting from the reaction. A device containing a photomultiplier tube may be used, for example.
In addition to the labelled antibodies or other such reagents specific to the analyte, a second type of labelled antibody or other such reagent may be provided specific to a control spot in an assay device. Application of the fluid sample to the assay device results in the second type of antibody binding to the control spot. This provides an indication that the assay device has been used, even if an insufficient amount of analyte is present in the fluid sample for detection of the analyte.
In addition to the labelled antibodies or other such reagents specific to the analyte and a second type of labelled antibody or other such reagent, a fluid dye may be added to the pre-lyophilization mix to confirm the presence and successful transfer of the dyed fluid sample to another vessel or assay device. A typical dye is Bromophenol Blue, Sigma Chemicals, St. Louis, Missouri 63178.
_7_ The concentrating material 45 absorbs liquid from the fluid sample, increasing the concentration of the analyte, such as bacteria, in the fluid sample. The concentrating material may be any material that absorbs fluid and does not react with the analyte in the fluid sample. Super-absorbent polymers, such as polyacrylates, cellulose derivatives and hydrogels, for example, are preferred. Super-absorbent polymers are discussed in Absorbent Polymer Technology, Studies in Polymer Science 8, edited by Lisa Brannon-Peppes and Ronald S. Harland, Elsevier Sciences, 1990, which is incorporated by reference herein, in its entirety. A suitable commercially available super-absorbent polymer is Favor-Pac 100, from Stockausen Inc., Greensborough, North Caroline, U.S.A. Favor~-Pac 100 is said to be a salt of crosslinked polyacrylic acid and grafted copolymer. According to the manufacturer, the carboxylic groups of the polymer are solvated when brought into contact with water or water based liquid. As a result, the groups partially dissociate into negatively charged carboxylic ions. In this state, the polymer chain contains a large number of similarly charged ionic groups that repel each other. The polymer coils become more bulky and thus extend their propensity to absorb aqueous fluid. Due to the cross-linking between the polymer chains, a gel is formed.
Water is strongly bonded by hydrogen bonds in the gel. The physical characteristics of the Favor~-Pac 100, as provided by the manufacturer, are:
Physical Form White granules Particle Size 100-850 microns Product Density 540g/ +/-30 Sifting Properties Free Flowing Moisture Content 5% +/-2 pH value ( 1 % gel in 0.9% NaCI) 6.0 +/- 0.5 Storage >1 year under dry conditions Thirty (30) milligrams of Favor~-Pac 100 has been found to be an appropriate amount of Favor~-Pac 100 to use with a fluid sample of about 300-350 microliters, to increase analyte concentration by a factor of three (3). Excessive concentration, such as 10 times or more, is generally not desirable. If the concentrating material is being provided with the lyophilized antibodies, it may be desirable to add additional antibodies to compensate for antibodies that may be absorbed by the concentrating material along with the fluid and for the initial high level of fluid in the sample. As mentioned above, _g_ the super-absorbent polymer particles have a size preferably greater than about 0.80 millimeters. Smaller particles may be removed using a mechanical separation process, such as a sieve. Another example of a super-absorbent polymer is carboxymethyl cellulose.
Depending on the sensitivity of the detection system and the concentration of the analyte in the fluid sample, concentration of the fluid sample may not be required.
The concentrating material 45 may be provided without the reagents. For example, concentration may be advantageous but it may be desired to label the analyte afterwards. The ana,lyte could be labelled in the assay device, for example.
In some applications, it is necessary to concentrate the fluid sample prior to labelling.
The present invention is not intended to be limited in scope by the specific embodiments described herein. Although the present invention has been described in detail for the purpose of illustration, various modifications of the invention as disclosed, in addition to those described herein, will become apparent to those of skill in the art for the foregoing description. Such modifications are intended to be encompassed within the scope of the present claims.

Claims (18)

1. A syringe applicator for transferring a fluid sample, comprising:

a syringe body defining a hollow bore with a open end and an outlet;

a plunger having a piston at one end, said plunger being effective to fit within said hollow bore, and a second end extending out of said open end when said piston is located within said hallow bore; and a reagent located between said piston and said outlet when said piston is located within said hollow bore.
2. The syringe applicator of claim 1, further comprising:

an inlet/outlet assembly, said assembly having being attached to said syringe body at said outlet.
3. The syringe applicator of claim 1, further comprising a seal between said outlet and said hollow bore.
4. The syringe applicator of claim 1, wherein said reagent is a member selected from the group consisting of an antibody linked to a detectable marker and a luciferin/luciferase complex.
5. The syringe applicator of claim 4, wherein said marker is a member selected from the group consisting of a heavy metal or a fluorescent, chemical, colorimetric or radioactive label.
6. The syringe applicator of claim 1, wherein said syringe contains a concentrating material, and said concentrating material is a superabsorbent polymer.
7. The syringe applicator of claim 6, wherein said superabsorbent material is a member selected from the group consisting of a polyacrylate, a cellulose derivative, Favor®-Pac 100, and a hydrogel.
8. The syringe applicator of claim 6, wherein said superabsorbent material is Favor®-Pac 100.
9. The syringe applicator of claim 6, wherein said superabsorbent material is carboxymethyl cellulose.
10. The syringe applicator or claim 1, further comprising a concentrating material located between said piston and said outlet when said piston is located within said hollow bore.
11. The syringe applicator of claim 10, wherein said superabsorbent material is a member selected from the group consisting of a polyacrylate, a cellulose derivative, Favor®-Pac 100, and a hydrogel, and said reagent is a member selected from the group consisting of an antibody linked to a detectable marker and a luciferin/luciferase complex.
12. The syringe applicator of claim 4, further comprising an antibody effective to bind to a control spot on an assay device.
13. The syringe applicator of claim 2, wherein said inlet/outlet assembly has a first end coupled to a flange that defines said outlet, and a second end capable of being inserted into a source of fluid material to withdraw a sample of said fluid material.
14. The syringe applicator of claim 13, wherein:

said reagent is a concentrating material, and said concentrating material includes particles of a superabsorbent polymer; and said inlet/outlet assembly includes a first tube having an inner diameter small than the size of the particles of said superabsorbent polymer; and said inlet/outlet assembly includes a second tube made of heat shrink tubing and capable of coupling said first tube to said flange.
15. A syringe applicator of claim 1 further comprising a cap attached to said syringe body and sealing said oulet.
16. A syringe applicator of claim 15 wherein the cap is attached to said syringe body by perforations.
17. A method of concentrating an analyte present in a liquid sample, comprising the steps of:
drawing at least a portion of said liquid sample into a syringe applicator;
said syringe applicator comprising a syringe body defining a hollow bore with a open end and an outlet; a plunger having a piston at one end, said plunger being effective to fit within said hollow bore, and a second end extending out of said open end when said piston is located within said hollow bore; and a concentrating material located between said piston and said outlet when said piston is located within said hollow bore;
mixing and incubating said portion with said concentrating material for a period of time sufficient to concentrate said portion by absorbing fluid into said concentrating material;
and expelling an amount of said portion through said outlet.
18. A method for reacting an analyte present in a liquid sample, comprising the steps of:
drawing at least a portion of said liquid sample into a syringe applicator;
said syringe applicator comprising a syringe body defining a hollow bore with a open end and an outlet; a plunger having a piston at one end, said plunger being effective to fit within said hollow bore, and a second end extending out of said open end when said piston is located within said hollow bore; and a reagent located between said piston and said outlet when said piston is located within said hollow bore;
mixing and incubating said portion with said reagent for a period of time sufficient to react said analyte in said portion with said reagent; and expelling an amount of said portion through said outlet.
CA 2474940 2004-07-16 2004-07-16 Method and device to process, label and concentrate analytes Abandoned CA2474940A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA 2474940 CA2474940A1 (en) 2004-07-16 2004-07-16 Method and device to process, label and concentrate analytes

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
CA 2474940 CA2474940A1 (en) 2004-07-16 2004-07-16 Method and device to process, label and concentrate analytes
CN 200580030712 CN101052866A (en) 2004-07-16 2005-07-18 Method and device for treating, marking and concentrating analysis
EP20050768271 EP1774277A1 (en) 2004-07-16 2005-07-18 Method and device to process, label and concentrate analytes
AU2005263153A AU2005263153A1 (en) 2004-07-16 2005-07-18 Method and device to process, label and concentrate analytes
JP2007520637A JP2008506930A (en) 2004-07-16 2005-07-18 Processing an analyte, a method and device for labeling and concentrated
PCT/CA2005/001129 WO2006007711A1 (en) 2004-07-16 2005-07-18 Method and device to process, label and concentrate analytes
CA 2573925 CA2573925A1 (en) 2004-07-16 2005-07-18 Method and device to process, label and concentrate analytes

Publications (1)

Publication Number Publication Date
CA2474940A1 true true CA2474940A1 (en) 2006-01-16

Family

ID=35636989

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2474940 Abandoned CA2474940A1 (en) 2004-07-16 2004-07-16 Method and device to process, label and concentrate analytes

Country Status (5)

Country Link
EP (1) EP1774277A1 (en)
JP (1) JP2008506930A (en)
CN (1) CN101052866A (en)
CA (1) CA2474940A1 (en)
WO (1) WO2006007711A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009155430A3 (en) * 2008-06-18 2010-12-16 The Cleveland Clinic Foundation Systems and methods for vitrifying tissue
JP2012513768A (en) 2008-12-31 2012-06-21 スリーエム イノベイティブ プロパティズ カンパニー The method for concentrating the sampling device and microorganisms
US7927563B1 (en) 2009-10-13 2011-04-19 Cytomedix, Inc. Kit for separation of biological fluids
WO2015098967A1 (en) * 2013-12-24 2015-07-02 東亜ディーケーケー株式会社 Reagent container, reagent-filled reagent container, reaction unit, and analysis system
CN104502150B (en) * 2014-12-16 2017-01-04 武汉海王新能源工程技术有限公司 Nuclear power plant with a radioactive liquid sample sampling apparatus and sampling method

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61194020A (en) * 1985-02-22 1986-08-28 Dai Ichi Seiyaku Co Ltd Remedy for retinopathy
GB8602732D0 (en) * 1986-02-04 1986-03-12 Univ Brunel Taking samples from patients
US6123687A (en) * 1996-10-30 2000-09-26 Cohesion Technologies, Inc. Cell separation device and metering syringe
US6187530B1 (en) * 1997-10-03 2001-02-13 Monterey Bay Aquarium Research Institute Aquatic autosampler device
DE19835721A1 (en) * 1998-08-07 2000-02-10 Edi Experimentelle & Diagnosti A method for determining the immune response of the blood and test kit therefor, and using a suitable syringe barrel

Also Published As

Publication number Publication date Type
CN101052866A (en) 2007-10-10 application
EP1774277A1 (en) 2007-04-18 application
WO2006007711A1 (en) 2006-01-26 application
JP2008506930A (en) 2008-03-06 application

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