CA2463594A1 - Method for unspecific enrichment of bacterial cells - Google Patents

Method for unspecific enrichment of bacterial cells Download PDF

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Publication number
CA2463594A1
CA2463594A1 CA002463594A CA2463594A CA2463594A1 CA 2463594 A1 CA2463594 A1 CA 2463594A1 CA 002463594 A CA002463594 A CA 002463594A CA 2463594 A CA2463594 A CA 2463594A CA 2463594 A1 CA2463594 A1 CA 2463594A1
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CA
Canada
Prior art keywords
spec
bacterial cells
magnetic
magnetic carrier
unspecific
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002463594A
Other languages
French (fr)
Other versions
CA2463594C (en
Inventor
Sabine Diller
Renate Grassl
Stefan Miller
Ingrid Robl
Michael Schuetz
Thomas Zander
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hyglos Invest GmbH
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Individual
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Filing date
Publication date
Priority claimed from DE2001149803 external-priority patent/DE10149803A1/en
Application filed by Individual filed Critical Individual
Publication of CA2463594A1 publication Critical patent/CA2463594A1/en
Application granted granted Critical
Publication of CA2463594C publication Critical patent/CA2463594C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method for unspecific enrichment of bacterial cells by means of cationic or anionic polymers and magnetic carriers.

Claims (7)

1. A method for the unspecific purification of bacteria comprising the following steps:
a) contacting the sample, containing bacterial cells, with a cationic polymer at an acidic pH- value, b) addition of a magnetic carrier, c) separation of the magnetic carrier with the hereon bound bacterial cells from the sample.
2. The method according to claim 1, wherein after step a) and/or step b) an incubation step is inserted.
3. The method according to any of the preceding claims, wherein the cationic polymer is chitosan or polylysine.
4. The method according to any of the preceding claims, wherein chitosan is added with a concentration in the range from 0.05 to 100 pg/ml culture and polylysine with a concentration in the range of 12.5 to 150 µg/ml culture.
5. The method according to any of the preceding claims, wherein the pH-value is adjusted with HCl/HAc to less than or equal to 3 or with formic acid to less than or equal to 4.
6. The method according to any of the preceding claims, wherein the magnetic carrier is a magnetic pigment or a magnetic polystyrene-bead, in particular with a NH2-, COOH-or OH-surface.
7. The method according to any of the preceding claims, wherein the purified bacteria are E. coli, Bacillus spec, Proteus spec, Micrococcus spec, Staphylococcus spec or Citrobacter spec.
CA2463594A 2001-10-09 2002-10-08 Method for unspecific enrichment of bacterial cells Expired - Lifetime CA2463594C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE10149803.9 2001-10-09
DE2001149803 DE10149803A1 (en) 2001-10-09 2001-10-09 Non-specific enrichment of bacteria, useful e.g. for plasmid or nucleic acid recovery, by precipitation with cationic polymer and magnetic carrier without centrifugation
DE10230147A DE10230147A1 (en) 2001-10-09 2002-07-04 Process for non-specific enrichment of bacterial cells
DE10230147.6 2002-07-04
PCT/DE2002/003790 WO2003033698A1 (en) 2001-10-09 2002-10-08 Method for unspecific enrichment of bacteria cells

Publications (2)

Publication Number Publication Date
CA2463594A1 true CA2463594A1 (en) 2003-04-24
CA2463594C CA2463594C (en) 2012-02-14

Family

ID=26010328

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2463594A Expired - Lifetime CA2463594C (en) 2001-10-09 2002-10-08 Method for unspecific enrichment of bacterial cells

Country Status (9)

Country Link
US (1) US7868144B2 (en)
EP (1) EP1434864B1 (en)
JP (1) JP2005505300A (en)
CN (1) CN1568367A (en)
AT (1) ATE512220T1 (en)
CA (1) CA2463594C (en)
DE (2) DE10230147A1 (en)
DK (1) DK1434864T3 (en)
WO (1) WO2003033698A1 (en)

Families Citing this family (15)

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Publication number Priority date Publication date Assignee Title
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
DE102005002342A1 (en) * 2005-01-18 2006-07-20 GEN-Institut für Angewandte Laboranalysen GmbH Method and kit for sterilizing microorganism-containing fluids
FR2883296B1 (en) * 2005-03-15 2007-05-18 Nicolas Bara METHOD AND DEVICE FOR ISOLATING MICROORGANISMS
KR100682945B1 (en) * 2005-05-24 2007-02-15 삼성전자주식회사 Cell Separation Method and Kit Using Phase Separation
JP4670583B2 (en) * 2005-10-20 2011-04-13 チッソ株式会社 Separation or detection method of lipid vesicles using water-soluble cationic magnetic fine particles
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
CA3187687A1 (en) 2007-09-14 2009-03-19 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US12529164B2 (en) 2007-09-14 2026-01-20 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
JP2011509083A (en) * 2007-12-31 2011-03-24 スリーエム イノベイティブ プロパティズ カンパニー Composition for capturing microorganisms and method for capturing microorganisms
US9834808B2 (en) 2016-01-21 2017-12-05 SeLux Diagnostics, Inc. Methods for rapid antibiotic susceptibility testing
CN109072280B (en) 2016-01-21 2022-10-14 赛录科试诊断公司 Method for rapid antimicrobial sensitivity testing
CN107894486B (en) * 2017-11-27 2020-05-12 利民化学有限责任公司 Analysis method of 2,3,6, 7-tetramethoxy-9-phenanthrenecarboxylic acid-S- (2-benzimidazole) ester
US11845976B2 (en) 2019-08-27 2023-12-19 SeLux Diagnostics, Inc. Systems and methods for performing antimicrobial susceptibility testing
EP4121528A1 (en) * 2020-03-17 2023-01-25 F. Hoffmann-La Roche AG Method of improving cell recovery in single-cell analysis
CN115521945B (en) * 2022-10-26 2023-09-12 南京大学 High polyphosphate and method for producing high polyphosphate by one-step fermentation

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE441009B (en) 1982-03-08 1985-09-02 Kjell Nilsson WAY TO IMMOBILIZE LIVING BIOMATERIAL IN PEARLY POLYMERS
US5076950A (en) * 1985-12-20 1991-12-31 Syntex (U.S.A.) Inc. Magnetic composition for particle separation
US5051189A (en) 1989-01-31 1991-09-24 University Of Florida Method of removing an unwanted impurity from an aqueous material
US5075430A (en) 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
US5279936A (en) * 1989-12-22 1994-01-18 Syntex (U.S.A.) Inc. Method of separation employing magnetic particles and second medium
DE4034036C2 (en) 1990-10-26 1994-03-03 Diagen Inst Molekularbio Device and method for isolating nucleic acids from cell suspensions
US5208166A (en) 1991-02-28 1993-05-04 Saunders Mary S Reactive chitosan coated articles and test kit for immunoassay
US5264184A (en) 1991-03-19 1993-11-23 Minnesota Mining And Manufacturing Company Device and a method for separating liquid samples
IL100096A (en) 1991-11-20 1996-03-31 Univ Ramot Method for entrapment of active materials in chitosan
GB9709728D0 (en) * 1997-05-13 1997-07-02 Dynal As Single step method
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
DE19851156C1 (en) * 1998-11-06 2000-04-27 Merck Patent Gmbh Isolation of plasmid DNA from a microbial culture comprises acidification and using particles to separate microbial cells from the culture
GB9929297D0 (en) 1999-12-11 2000-02-02 Glaxo Group Ltd Process
KR100375422B1 (en) 1999-12-21 2003-03-10 한국과학기술연구원 Macroporous chitosan beads and preparation method thereof
EP1110610A1 (en) 1999-12-23 2001-06-27 3M Innovative Properties Company Micro-titer plate with filter inserts and method of making same
GB0001450D0 (en) 2000-01-21 2000-03-08 Genpoint As Cell isolation method
EP1118676A2 (en) 2000-01-21 2001-07-25 Chemagen AG Cell isolation method

Also Published As

Publication number Publication date
ATE512220T1 (en) 2011-06-15
DE10230147A1 (en) 2004-01-15
DK1434864T3 (en) 2011-10-03
US7868144B2 (en) 2011-01-11
EP1434864A1 (en) 2004-07-07
CA2463594C (en) 2012-02-14
US20050019827A1 (en) 2005-01-27
DE10294716D2 (en) 2004-09-02
CN1568367A (en) 2005-01-19
EP1434864B1 (en) 2011-06-08
JP2005505300A (en) 2005-02-24
WO2003033698A1 (en) 2003-04-24

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Effective date: 20221011